LOCUS       CP040448                5251 bp    DNA     circular BCT 30-JUN-2020
DEFINITION  Klebsiella pneumoniae strain WCHKP115032 plasmid p2_115032, complete
            sequence.
ACCESSION   CP040448
VERSION     CP040448.1
DBLINK      BioProject: PRJNA353728
            BioSample: SAMN09842420
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain WCHKP115032
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/17/2019 14:22:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,587
            CDSs (total)                      :: 5,457
            Genes (coding)                    :: 5,337
            CDSs (with protein)               :: 5,337
            Genes (RNA)                       :: 130
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 52 of 120
            Pseudo Genes (incomplete)         :: 69 of 120
            Pseudo Genes (internal stop)      :: 19 of 120
            Pseudo Genes (multiple problems)  :: 18 of 120
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP115032"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_115032"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2018"
     gene            231..572
                     /locus_tag="D0S97_27900"
     CDS             231..572
                     /locus_tag="D0S97_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QLB33787.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPAR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGGDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            535..1272
                     /locus_tag="D0S97_27905"
                     /pseudo=""
     CDS             535..1272
                     /locus_tag="D0S97_27905"
                     /inference="COORDINATES: protein motif:HMM:PF03432.12"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /name="nuclease"
     gene            1250..1735
                     /locus_tag="D0S97_27910"
     CDS             1250..1735
                     /locus_tag="D0S97_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QLB33788.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="mobilization protein"
     gene            1739..1951
                     /gene="mbeD"
                     /locus_tag="D0S97_27915"
     CDS             1739..1951
                     /gene="mbeD"
                     /locus_tag="D0S97_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QLB33789.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="mbeD"
     gene            2584..3939
                     /locus_tag="D0S97_27920"
     CDS             2584..3939
                     /locus_tag="D0S97_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="QLB33790.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATESGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRVKANLFTRDESDTWETIKFGGHYKGG
                     IKKIPFFPNNSYLAKAGNNAASPDIIKEAYNFFRKGIRHIGLNEKIRISSFGEREDVVN
                     ETAKVLCMVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFSHPTDEGG
                     SVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKTTTPYAAW
                     YDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE"
                     /name="ATP-binding protein"
     gene            3896..4552
                     /locus_tag="D0S97_27925"
     CDS             3896..4552
                     /locus_tag="D0S97_27925"
                     /inference="COORDINATES: protein motif:HMM:PF13707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB domain-containing protein"
                     /protein_id="QLB33791.1"
                     /translation="MVFLKSLTGIFLMSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYI
                     SDRSKRTLSVFKIPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVVYDQEHLTPSSISC
                     HQKAWNKANRHGINIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKG
                     SKLTYLAIRERIGTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB domain-containing protein"
     gene            complement(4705..4866)
                     /locus_tag="D0S97_27930"
     CDS             complement(4705..4866)
                     /locus_tag="D0S97_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QLB33792.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVSLE
                     VLRQLRLN"
                     /name="replication initiation protein"
     gene            complement(4891..5208)
                     /locus_tag="D0S97_27935"
     CDS             complement(4891..5208)
                     /locus_tag="D0S97_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001692121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLB33793.1"
                     /translation="MPLLPFGCGGRYLHTEKSEGIWHDKRGRKRMRRCRFSIGSAPLTS
                     ITKSDAQISGGETRQDYKDTRRFPLVAPSCALLFLPFGLPVSFRCYGRVCLIPRLTLSS
                     G"
                     /name="hypothetical protein"
     Typing          61..145
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          complement(4754..5251)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.8"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          563..2067
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.618"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttctgcctt acgcccgcag ctgcattttc ctctgtcaca tgaagcactt tcctgattac
       61 cgcactcatg ccaacataat aagccagtat acactccgct agcgctacgt gactggttca
      121 gggctgcgcc ccgaaacccg ctaaaagcga ctgacgcgcc tgcggcttgt ccaacaccgt
      181 gccgaccggg aaacatttcc cgcccgtccc ggtgttatca ggaggctact ctggcagaca
      241 aacgcaacaa gatgctcacg atgtgggtga cggaagacga acaccggcgg ctgctcgagc
      301 ggtgcgacgg caagcagctg gcggcgtgga tgcgtcagac ctgcctcgat gagaagccgg
      361 cgcgtgccgg cagactgccg tcgatatcgc cggcgctgct gcgccagctt gccggtatgg
      421 ggaacaacct gaaccagatc gcccggaaag tgaatgccgg tggtgccggt catgaccggg
      481 tgcagattgt ggccgcactg atggcaatcg acgcgggact ggagcggctg cggcatgcgg
      541 tgctggaaaa agggggggat gatgatcgtt aaatttcacc cccgggggcg cggtggtggt
      601 gccggtccgg tggattacct gctgggaaaa gaccggcagc gcgagggggc gaccgtgctg
      661 cagggaaaac cggaggaagt ccgggagctt atcgatgcct cgccgtatgc gaaaaagtac
      721 acctccggcg tgctgtcgtt cgcggaggct gaactgccgc ccgggcagcg ggagcagatc
      781 atggccagct ttgagcgggt tctgatgccc gggctcgata aagaccagta cagcattctg
      841 tgggtggagc acaccgacaa ggggcggctg gagctgaatt tcctgatccc caacactgaa
      901 ctgctgaccg ggaaacgcct gcagccgtac tacgatcgcg ccgaccggcc gcgcatcgat
      961 gcctggcaga ccgtggtgaa cggcagactg gggctgcatg atccaaacgc cccggagaac
     1021 cggcggttac tggtcacgcc gtccgcactg ccggaaacga agctggaagc cgctcaggcg
     1081 attacacggg ggttgctggc cttagcctca tccggggagc taaaaacgcg cgaggacgtc
     1141 acaggggcgc tcacagcggc aggttttgag gtggtccgca ccacaaaaaa cagtatcagc
     1201 attgccgacc cggacggggg gcgaaacatc cgactgaagg gagccattta tgagcagtct
     1261 tttagcgctg gcgaaggact tggagcagca atcgaaagcg cagcagcaga gtaccggcga
     1321 gatgctgaaa gccgcattca gcgagcacga gcagtctgta aaagcggaac tgagcgcaag
     1381 cgcgaagaga atcagcgacg ccatcagcgc ccacgagcag ggtatgaccg cggcgatgca
     1441 gtcgaaccgg ctgagcgtgc tgcgtatggt cggccggacg tggctgacca tcaccctggt
     1501 ctcggtactg ctgatcgcca cgagcgggag cattctgtgg tggcaggggc agcagatcac
     1561 cgggaattac cagactatcc gggcgcagga gcggacgcag gccatgctgt cggagaagaa
     1621 ccacggcgtg cagctgtcgc tctgcgggga gaagaaactg tcctgcgtca aagtgaaccc
     1681 gaaagcggga gcttatgggg aggaagggaa ctggatggta ctggagagga aatagcccat
     1741 gacggaactg gaaaaacagt tgctgaccgc attagagcag ttacatcagg actactcgca
     1801 aaggctggac gagtgggaga gcgccttcgc ggaatggcag aagatgtctg gtcttatgca
     1861 acgggagaac gcggcgctga acgagcgcgt cacgcgcttg agtcagcagg tggcgaactt
     1921 gagcaggcag ttacagcgtt tgagccagta gtgcagcggc atgaaatggc ggtggccgcc
     1981 gaacgggcat acgagcagaa acagcatgaa aaagagctgg ctgaaacccg ttcccggcag
     2041 cggagctata acggtccgac gctgggctga ctgcgcgcat ccagtgacaa accccaagaa
     2101 gaagggctca taaatttccc gctaaatacg gtcgtgatgt ttccatcaca gcgtatacaa
     2161 cggagggatt atgagactgt ttaacgtgat tgagacggtg ctgaactgcc tgtacatggc
     2221 gctggagatc tgggactggc tggagaagca ttttcctgtt tttaaggcgt tcatccggat
     2281 tctggcctga aatcagaccg tatgcatctg gctgcatgac catgcagccg ggtaaaattt
     2341 atctgtcacg aacggggcgg tgtatcgggt ggtttgttgc cggttttacc tgactgccgc
     2401 cccgtttgtg gcgaacctgt ccggagcggt gcgggcaaca gatgttatgg taaatagcgt
     2461 gtaattataa atagcacaac gttttttaag gaagatgttg ttccaaaatt aaaacatata
     2521 ggatatcttt cctatgattg atttttcgct tttttggcga aaaatccccg ttaggaggta
     2581 tgcatgtttg tttatttgac tattgaaaat tttaggtcgg ttaaggaaaa atcgacatta
     2641 aacttgagcg catccggttc tagtaaccat ttaacaactc atatctataa aaatgatgga
     2701 atgaatgttg gaacgctcat gagtgctggg atttatggtg ctaatgcgtc tggtaaatct
     2761 aacgttctca tggcttttga agctataaaa tttatcgcaa cagaaagcgg tgatttgaaa
     2821 gaaggtaaac ggattccctg ctatgagcca tatgctcttt cttcggctac gaatgaggct
     2881 cctgtaaagt ttgaggctga attttattct gttgagggga atcggttcag ttatgaagtt
     2941 gcttttatta aaaatcggat tttatttgaa tcattagact attatccatc tagggttaaa
     3001 gcaaatttat ttactagaga tgagagcgac acctgggaaa caatcaagtt tggtgggcat
     3061 tataaaggag gtattaaaaa aataccattc ttccctaata attcatactt agcgaaagca
     3121 ggtaataatg ccgcatcacc agacataatt aaagaagctt ataacttttt tagaaaggga
     3181 attagacata taggtttaaa tgaaaagata agaatatctt cttttgggga gagggaggac
     3241 gtcgtaaatg aaacggccaa ggttttatgc atggttgaca ctggtgtttc cggtatatct
     3301 gtacgggagg ttgaggcgga aataccaata aaaatggatg aggaaatccc tcaagaaata
     3361 aaggagataa ttgaagaaga ttataaatat cgttatatgt tctctcatcc tactgatgaa
     3421 ggggggagtg tttctttccc tatggggaga gagtctgaag gcacccaaaa attatttgaa
     3481 atcatacctc taattagatc tgcgtttaat gatagtatgg ttgtaattgt tgatgagtta
     3541 gataatagct tgcacccgca tattgctgac ttgattgtta agttatttaa tgatcctgat
     3601 gtcaataaaa aaggctcaca acttgtattc tcaacccata atatgcagtt aatggctcca
     3661 gagaaaatga gaagagatca gatatggttt tgtgaaaaaa ataaaggtgc gtcgtctctt
     3721 tatagcttag atgattttga taagaaaaag ataaaaacaa caacacctta cgcagcttgg
     3781 tatgatgagg gtaggtttgg tggggttccc gacataaact acttaaaggt agcctcattt
     3841 atatctggtg atatatcctt ggtaatgccg gatattgacg tcaaagaatt gtctgatggt
     3901 ttttttgaag agtttgacgg ggatctttct gatgagtaaa aaacatggaa ataataagaa
     3961 agtcccacaa gtaatgtata tatactgtga gggtgagaaa accgaaccat tataccttga
     4021 ggcttatatt tcagacagat ctaaaagaac tttaagtgta tttaaaatac ctaaaacaag
     4081 gaaaaataca cctgagcagt tggtggatga ggcgataaag aaaaagaatt catcatcttc
     4141 cgcagaaggt gacgtctttt gggttgttta tgatcaagag catctgacgc cttcgtctat
     4201 ttcatgtcat caaaaggcat ggaataaagc taataggcat gggataaata tagcaatatc
     4261 ctgtgtttgt tttgaattat ggttattgtt gcatttcggc ttcacaacca aagatttttc
     4321 gtcgtatgat aatttgatgt ctagctcccc gctaaaaaaa ttaataacta actatgataa
     4381 aggtagtaaa ctaacttacc ttgctataag agagaggatt ggtactgcga aagataacgc
     4441 aagaaagcta gatatttacg ctaaaactaa ttataaaaac acgcacgaac cttatctatt
     4501 tccatgttat acatcattcc atgagctact taaggcgatt gacgactttt gaagtaatcc
     4561 attgaccatg gcttattaca ggctgtaatc tatgaaaaat aaaaaccacc gctaccaacg
     4621 gtggtttttg gaaggtacat gagctaccaa cttatgtacc gggtaactgg cttagaggga
     4681 gcgcagtcac caaaaattgt cctttcagtt tagccttaac tggcgcagaa cttcaagact
     4741 cacttcctct aatccgttac caatggctgc tgccagtggc gatgaaccgt gtctttccgg
     4801 gttggactca agatgatagt taccggagaa ggcgcagcgg tcggactgaa cggggggttc
     4861 gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat
     4921 gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag
     4981 agcgcacgag ggagccacca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5041 gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga
     5101 aaaacggcag cggcgcatcc tttttctgcc ccgtttgtca tgccatatgc cttcgctctt
     5161 ctccgtgtgc aggtaacgcc cgccgcagcc gaacggcagg agcggcagcg actgagtgag
     5221 cgaggaggcg tcatatcctc tgaacttcca t
//
