LOCUS       CP037998                8401 bp    DNA     circular BCT 26-MAR-2019
DEFINITION  Shigella sonnei strain FC1653 plasmid unnamed1, complete sequence.
ACCESSION   CP037998
VERSION     CP037998.1
DBLINK      BioProject: PRJNA414945
            BioSample: SAMN07811411
KEYWORDS    .
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Anandan,S., Muthuirulandi Sethuvel,D.P., Devanga Ragupathi,N.K.,
            Veeraraghavan,B., Neeravi,A., Venkatesan,M. and Vasudevan,K.
  TITLE     Complete genome sequence of S. sonnei using hybrid genome assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Prabaa,D., Shalini,A., Kumar,N. and Balaji,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2017) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore 632004, India
REFERENCE   3  (bases 1 to 8401)
  AUTHORS   Anandan,S., Muthuirulandi Sethuvel,D.P., Devanga Ragupathi,N.K.,
            Veeraraghavan,B., Neeravi,A., Venkatesan,M. and Vasudevan,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method :: Canu v. 1.7; Unicycler hybrid v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 174.0x
            Sequencing Technology             :: Oxford Nanopore; IonTorrent
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/15/2019 14:26:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,932
            CDSs (total)                      :: 4,806
            Genes (coding)                    :: 3,949
            CDSs (with protein)               :: 3,949
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 857
            CDSs (without protein)            :: 857
            Pseudo Genes (ambiguous residues) :: 0 of 857
            Pseudo Genes (frameshifted)       :: 495 of 857
            Pseudo Genes (incomplete)         :: 335 of 857
            Pseudo Genes (internal stop)      :: 117 of 857
            Pseudo Genes (multiple problems)  :: 84 of 857
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="FC1653"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /plasmid="unnamed1"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2017"
                     /collected_by="Christian Medical College"
     gene            complement(join(7589..8401,1..3))
                     /gene="sul2"
                     /locus_tag="CSW73_024525"
     ARG             complement(7593..8401)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="99.63"
     gene            complement(463..621)
                     /locus_tag="CSW73_024530"
     CDS             complement(463..621)
                     /locus_tag="CSW73_024530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QBQ28120.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="replication initiation protein"
     gene            complement(663..1067)
                     /locus_tag="CSW73_024535"
     CDS             complement(663..1067)
                     /locus_tag="CSW73_024535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /protein_id="QBQ28121.1"
                     /translation="MLHVAGEKRQHRRVSRICDTGDIPLPRSLTRYARSFGCGERYRLT
                     DGDGNFLEDTRKTLSKEEIRPRRSRFSIGSAPLTSIAKSDAQISGGETRQDYKDPRRFP
                     LVAPSCALLFLPFGLPVSFRCYGRGFSFHA"
                     /name="plasmid replication initiation protein"
     gene            complement(1732..1962)
                     /locus_tag="CSW73_024540"
     CDS             complement(1732..1962)
                     /locus_tag="CSW73_024540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QBQ28122.1"
                     /translation="MHEYAHKCIARKFTPFSRGKSCFRRSSHPKTVNGDAIYCVAVKRC
                     RRSRKTRCQHVQEGGDVQMAQRMKVTYELKE"
                     /name="hypothetical protein"
     gene            complement(2108..2596)
                     /locus_tag="CSW73_024545"
     CDS             complement(2108..2596)
                     /locus_tag="CSW73_024545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QBQ28123.1"
                     /translation="MFCCYNTVHTGDTVTFMAWHTVQETLKLTGKSRSQLYRDMAKGLV
                     SYRTGKNDRREFETSELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDA
                     LQQTVTLMLEDKMAREAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="entry exclusion protein 1"
     gene            2766..3155
                     /locus_tag="CSW73_024550"
     CDS             2766..3155
                     /locus_tag="CSW73_024550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QBQ28124.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            3199..3387
                     /locus_tag="CSW73_024555"
                     /pseudo=""
     CDS             3199..3387
                     /locus_tag="CSW73_024555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial on complete genome; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(3354..4553)
                     /gene="tet(A)"
                     /locus_tag="CSW73_024560"
     ARG             complement(3358..4553)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4632..5309
                     /locus_tag="CSW73_024565"
     CDS             4632..5309
                     /locus_tag="CSW73_024565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="QBQ28126.1"
                     /translation="MFISDKVSSMTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGV
                     QQPALYWHFRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRD
                     GARIHAGTRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSD
                     AGERGGTVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRK
                     GDD"
                     /name="TetR family transcriptional regulator"
     gene            complement(5341..5583)
                     /locus_tag="CSW73_024570"
     CDS             complement(5341..5583)
                     /locus_tag="CSW73_024570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="QBQ28127.1"
                     /translation="MKTAATDLARERRAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="relaxase"
     gene            complement(5782..5985)
                     /locus_tag="CSW73_024575"
                     /pseudo=""
     CDS             complement(5782..5985)
                     /locus_tag="CSW73_024575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028401.1"
                     /note="incomplete; partial on complete genome; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein (MobL-like)"
                     /name="mobilization protein (MobL-like)"
     gene            complement(5889..6725)
                     /locus_tag="CSW73_024580"
     CDS             complement(5889..6725)
                     /locus_tag="CSW73_024580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="QBQ28128.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(6725..7528)
                     /gene="aph(3'')-Ib"
                     /locus_tag="CSW73_024585"
     ARG             complement(6729..7528)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     ARG             complement(5893..6725)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          984..1157
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(455..1060)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 catgggggct tctataataa taataatcga gcatgagtct catacggatg ctcgggtcga
       61 aagggaatcc ccaggcgagt aacctgtttg cggtgatccg tttcatgatg atgtatctgt
      121 agcattttgg aaataaatat atcatctctc aataagtaga tatatttatc ttttttgctt
      181 ctttggtgct gcaagtcacc aatacgaggg aaacctgtta cggagcggtt taaccgtaat
      241 caacagtgac tggtgtcatg ttcggaagat taattgatag taatctacaa aggatcttct
      301 tgagatcctt ttcaatgaat cgtaatctct tgctctgtaa acgaaaaaac cgcctgggga
      361 ggcggtttgt tcgaaggtta agtcagttgg ggaactgctt aacccggtaa cagggttcac
      421 gagaccgcca ttaccaaaac tgttctttca gtgtagccgc agtcaggcct ctacttcaag
      481 acctcttaat ctatcttctc gtgcactcct gttactaatg gctgctgcca gtggcgtttt
      541 gtcgtgtctt tccgggttgg actcaagttg atagttaccg gacaaggcgc agcggtcgga
      601 ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgccttca cggaactgag
      661 tgtcaggcgt ggaatgagaa accgcggcca taacagcgga atgacaccgg taaaccgaaa
      721 ggcaggaaca ggagagcgca cgagggagct accaggggga aacgcctggg atctttatag
      781 tcctgtcggg tttcgccacc actgatttga gcgtcagatt ttgcgatgct tgtcaggggg
      841 gcggagccta tggaaaaacg gcttcgccgt ggccttatct cttctttgct aagtgtcttc
      901 ctggtatctt ccaggaaatt tccgtccccg tccgtaagcc ggtaccgctc gccgcagccg
      961 aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa tatctcctgt atcgcatatt
     1021 ctgctgacgc gccggtgctg ccttttttct cctgccacat gaagcacttc actgattttc
     1081 gcaatcgtgg caacatagta agccagtata cactccgcta gcgctacatg actggttcag
     1141 ggctgcgccc cgaaacccgc taacaacctc tcagctactg gatatcaccc catgccatct
     1201 gaatatgatg aacaactgga ttcgactgat acgcagcttc ccggcgaata tgaccttttt
     1261 gatttaatgg cgcaggttac tgaagagaac ctgcatgccc ctgtggattt tgggtcgcct
     1321 gtcggccggg aagcgttata gctttttcgc ttgcccgcgt acgtggtaat actccggtat
     1381 taccttttat ttctttattt tcatgtaatt acagtggatt tttaataccc gggtattact
     1441 cgtgaaaacg aaatattcct tttttttctt ttaattcatg cagttgcgat gaaaatcgcg
     1501 gctgcgttag gtgtatgacc attttgaggt tgaaaaataa tcaaccatgg cgggtgattt
     1561 acgcaggtta aggggagatc gtgacaaaaa aagcgttcct cactgtatcg ttcatgcctg
     1621 catgaacggg ttattcttcc tgactacaga attacatcgc tccttcagtt catacatcac
     1681 ttttacatca cttatacatc acttttacat cacttataca tcacttttac atcattcctt
     1741 cagctcatac gtcactttca tcctctgtgc catttgtaca tcaccccctt cctgaacgtg
     1801 ctgacagcgg gttttccggc tgcgccggca acgcttcacg gccacgcaat aaattgcgtc
     1861 cccgttcacc gttttcggat gggaagaacg ccggaaacag gacttacccc ggctgaatgg
     1921 tgtgaatttc cgcgctatgc acttgtgcgc atactcatgc atgccgtaaa aacagagcct
     1981 gcgcgtttct ggcgggtttt cgggtggttt gttgcctgtt ttaccggttt cccgtcagaa
     2041 acgccctgag ggcctctcag gcggtgctga taacagatgt tatggtaaat cagagtcacc
     2101 tgcactgtta ctctgaaccg gagcgaaaca gccgcgacca gaatccccgc tttttttcct
     2161 gtgccagcgc gtcagccaga cggctaattt cggcctgtag ttgttccctc tcctcatttc
     2221 gcaggcggtt ttctgcttcc cgcgccatct tgtcttcaag cattagggta acagtctgct
     2281 gcagagcgtc cagtttctgc tgaatgacgt ctagctgagc attttccgcc tggctaacga
     2341 cagatttgga gtgtccctcc ttgtgccttt ttggtgtctc agaagtgcgc agctcgccat
     2401 aggcccgaat cagttcactg gtctcgaact cccggcgatc attttttcct gtgcgatagc
     2461 tgacaaggcc tttagccatg tcacgatata attgcgagcg cgactttcct gttaatttca
     2521 gtgtctcttg aactgtgtgc catgccataa aagtgacagt gtctcctgtg tgtaccgtat
     2581 tgtaacagca gaacatatta ctgttttaac acaaaaggtc tgaggctgcc cgtgaggcgt
     2641 gaaaaataca caaaaaacct gttgacggtg tggttttgac gggcgtagta tttcgaatac
     2701 tgtatataaa cacattgtta taaatacagt ttttgctgtt tttaaaagtc aaagaggata
     2761 attttatgag tacagagtta aatagcggta ctgtcggtgg ttttggtcac atgggaccaa
     2821 atgaccccta caatgatggt catagcggct ctggaagttc gggaggtagt tttaacggtg
     2881 gtggtaatgg tggccatgat aatagtagtg gttcatctta tactgcatcc tttggtctca
     2941 tcactggccg acacccgata actgtcccag gtacgaagaa tattaattct gtcgatcttt
     3001 ctgctgtcat taataacatt atacatcatg aaggtgcacg caataacatg tacctagata
     3061 ctgcgaacaa tgtaagagtg ggagtgacgg gcactggctg gcaatgtcta gcaacggcag
     3121 gcatttcggc tgagggtaaa agaactttcc gctaagcgat agactgtatg taaacacagt
     3181 attgcaagga cgcggaacat gcctcatgtg gcggccagga cggccagccg ggatcgggat
     3241 actggtcgtt accagagcca ccgacccgag caaacccttc tctatcagat cgttgacgag
     3301 tattacccgg cattcgctgc gcttatggca gagcagggaa aggaattgcc gggctatgtg
     3361 caacgggaat ttgaagaatt tctccaaagc ccgcgacgca gcgccggcag gcagagcaag
     3421 tagagggcag cgcctgcaat ccatgcccac ccgttccacg ttgttataga agccgcatag
     3481 atcgccgtga agaggagggg tccgacgatc gaggtcaggc tggtgagcgc cgccagtgag
     3541 ccttgcagct gcccctgacg ttcctcatcc acctgcctgg acaacattgc ttgcagcgcc
     3601 ggcattccga tgccacccga agcaagcagg accatgatcg ggaacgccat ccatccccgt
     3661 gtcgcgaagg caagcaggat gtagcctgtg ccgtcggcaa tcattccgag catgagtgcc
     3721 cgcctttcgc cgagccgggc ggctacaggg ccggtgatca ttgcctgggc gagtgaatgc
     3781 agaatgccaa atgcggcaag cgaaatgccg atcgtggtcg cgtcccagtg aaagcgatcc
     3841 tcgccgaaaa tgacccaaag cgcggccggc acctgtccga caagttgcat gatgaagaag
     3901 accgccatca gggcggcgac gacggtcatg ccccgggccc accggaacga agcgagcggg
     3961 ttgagagcct cccggcgtaa cggccggcgt tcgcctttgt gcgactccgg caaaaggaaa
     4021 cagcccgtca ggaaattgag gccgttcaag gctgccgcgg cgaagaacgg agcgtggggg
     4081 gagaaaccgc ccatcagccc accgagcaca ggtcccgcga ccatcccgaa cccgaaacag
     4141 gcgctcatga agccgaagtg ccgcgcgcgc tcatcgccat cagtgatatc ggcaatataa
     4201 gcgccggcta ccgccccagt cgccccggtg atgccggcca cgatccgccc gatatagaga
     4261 acccaaagga aaggcgccgt cgccatgatg gcgtagtcga cagcagcgcc ggccagcgag
     4321 acgagcaaga ccggccgccg cccgaaacga tccgacagcg cgcccagcac aggtgcgcag
     4381 gcaaattgca tcaacgcata cagcgccagc agaatgccat agtgggcggt gacgtcgttc
     4441 gagtgaacca gatcgcgcag gaggcccggc agcaccggca taatcaggcc gatgccgaca
     4501 gcgtcgagcg cgacagtgct cagaattacg atcaggggtc tgttgggttt cacgtctggc
     4561 ctccggacca gcctccgctg gtccgattga acgcgcggat tctttatcac tgataagttg
     4621 gtggacatat tatgtttatc agtgataaag tgtcaagcat gacaaagttg cagccgaata
     4681 cagtgatccg tgccgccctg gacctgttga acgaggtcgg cgtagacggt ctgacgacac
     4741 gcaaactggc ggaacggttg ggggttcagc agccggcgct ttactggcac ttcaggaaca
     4801 agcgggcgct gctcgacgca ctggccgaag ccatgctggc ggagaatcat acgcattcgg
     4861 tgccgagagc cgacgacgac tggcgctcat ttctgatcgg gaatgcccgc agcttcaggc
     4921 aggcgctgct cgcctaccgc gatggcgcgc gcatccatgc cggcacgcga ccgggcgcac
     4981 cgcagatgga aacggccgac gcgcagcttc gcttcctctg cgaggcgggt ttttcggccg
     5041 gggacgccgt caatgcgctg atgacaatca gctacttcac tgttggggcc gtgcttgagg
     5101 agcaggccgg cgacagcgat gccggcgagc gcggcggcac cgttgaacag gctccgctct
     5161 cgccgctgtt gcgggccgcg atagacgcct tcgacgaagc cggtccggac gcagcgttcg
     5221 agcagggact cgcggtgatt gtcgatggat tggcgaaaag gaggctcgtt gtcaggaacg
     5281 ttgaaggacc gagaaagggt gacgattgat caggaccgct gccggagcgc aacccactca
     5341 ctacagcaga gccatgtaga caacatcccc tccccctttc caccgcgtca gacgcccgta
     5401 gcagcccgct acgggctttt tcatgccctg ccctagcgtc caagcctcac ggccgcgctc
     5461 ggcctctctg gcggccttct ggcgctcctg ctgcggcgtc cgctcgtggg ccgtggcgcg
     5521 ggtccgcgcg ccggcctcgt gcgcccggcg ctcgcgggcg aggtccgtgg cggccgtctt
     5581 cacgttctgc cttgcgcaga tgagatagat ctagcgtgga ctcaaggctc tcgcgaatgg
     5641 ctcgcgttgg aaactttcat tgacacttga ggggcaccgc agggaaattc tcgtccttgc
     5701 gagaaccggc tatgtcgtgc tgcgcatcga gcctgcgccc ttggcttgtc tcgcccctct
     5761 ccgcgtcgct acggggcttc caggggtcgt ttgcgggaag gggcggaatc ctacgctaag
     5821 gctttggcca gcgatattct ccggtgagat tgatgtgttc ccaggggata ggagaagtcg
     5881 cttgatatct agtatgacgt ctgtcgcacc tgcttgatcg cggccgcgat agctagatcg
     5941 cgttgctcct cttctccatc cgcgttccaa gctgcggaaa ggcacccata agcgtacgcc
     6001 tggtcgagca ggcgacgcgg atcgacgtcc agcgcacgag agaatgcgtc cgccatctgt
     6061 gcaatgcgtc taggatcgag acaaaggtcg tctctgtcag ccggatcgta gaacatattg
     6121 gcggcgccaa agcccacttc accgaccaga ccgacgggat ctatcaccag ccagccgcga
     6181 ctggagaaca tgatgttttc atgatgcaga tcgccatgta gcccacgcag ttccgaggca
     6241 ttgctcatca tttgatcggc tataatcgcc gcgtggacgt agtcagtttg acaacctgcg
     6301 ttttgatcat cgcgcgcccg ctgaaacaaa gctgcaaagc gatcccggat cgggagaagg
     6361 gcagaaggca ggggttcctc agatgcggca tacagcttcg ccattagttc cgctgcaatt
     6421 tcggtcgcct ggtagtcgcc gtgctcggca acgatgtgag agagcattcg ctccccggca
     6481 tattcgagca acatcagatt gttctcacga ccgagcaacc ggactgctcc cctcccattg
     6541 cgccatacca gatagtcggc cccgcgcagt tcatcagcaa tgtcttctat aggtttcaat
     6601 cccttgacga ttgcaggagt cccgtctggc aatgaaactt tccaaacgag gctggaaaag
     6661 gtgtccgcaa tgagaacagg ttgcgaaacg tgccaatgag caggaaaaac aggcggcatg
     6721 aacatcaacc ccaagtcaga gggtccaatc gcagatagaa ggcaaggcgt tcgcggtcgg
     6781 gggcttcgat ccccaataca ttgaatagga cagcgaaggc gcgctctgct tcatctggcg
     6841 ctgcccagtt ctcttcggcg ttagcaatca tgagtgccaa atcggcatag cgatctgctg
     6901 ttccgagccg cccaaggtcg atcagacccg tgcattgaag agttttaggg tccaccatga
     6961 agttcggcat gcagggatca ccatggcaaa caaccatatc ggtgcgctct tggtcgagcc
     7021 gcaccggtag ctctcgttcg acacgagcca aaagatcgag ctgcggcgta ctcttgtcct
     7081 cgtccggtaa gaagtcggga ttgacggcat tgcgggacac cacatcaacg gcgcgtccga
     7141 acattcgcga cagcctgcgc tcaaactgac attgatcaac cgataggctg tgaacagcgc
     7201 caagttgctg ccccattgac ggccacgctt tgagcaaatc cgctccagac agatcagccg
     7261 ccggtactcc cggaattgcc gttatcacca agcatgcacc ctcctgttcc tcctgccagt
     7321 tgatgacctc ggggcaagcc acacctcgac ctttgagcca aatgaggcgg tcacgctctc
     7381 cagcgagctc accgcggcgg gaagcaggtg cgattttcgc gaaggcatgc ccgtcaccac
     7441 gtcgaaaaac aaaatcacca gattctccgc ctctgacagg caaccagtca gaatgcgatt
     7501 caccaaaaaa aatattagtt cgattcaatg gaggttcctt cagttttctg atgaagcgcg
     7561 aatatagaga aatatcccga atgtgcagtt aacgaattct tgcggtttct ttcagcgccg
     7621 ccaataccgc cagcccgtcg cgcaaggggc gcggctcgtg tgtgcggatg aagtcagctc
     7681 cacctgcggc ggcggcaagc tctgcagcga gtgtcgcggc cccgacatcc cccggaccac
     7741 ggcctgtgag cgcgcgcaga aaggatttgc gcgaaacaga cagaagcacc ggcaaatcga
     7801 agcgcagccg caattcatcg aaccgcgcca gcaccgagag cgaggtttcg ggagcagccc
     7861 ccagaaaaaa ccccatgccg ggatcaagga caaggcggtt gcgtttgata ccggcacccg
     7921 tcagcgccgc gatgcgcgcg tcaaagaacg ccgcaatgtg atccatgatg tcgccagcgg
     7981 gtgcctcgcg ccgatctgcc tgcccgtctt gcaccgaatg cataacgacg agtttggcag
     8041 atgatttcgc caattgcgga tagaacgcag cgtctggaaa accgcgaata tcattgagat
     8101 aggccacacc acgcgacaag gcataggctt gcgtcgcggg ttgataactg tcgagcgaga
     8161 cgggaatgcc atctgccttg agcgcgtcca gcaccggcgc gatacgcgcg atttctgtgt
     8221 cggacgaaac aggcgcggcg tcgggattgc tggatgccgg accgaggtcg atcacatctg
     8281 ccccctcggc catcagctta cgcgcctgcg caatggctgc gtctggcgcc agataccggc
     8341 ctccatcgga gaaactgtcc gaggttatgt tgacgatgcc gaaaatgatg agcgatttat
     8401 t
//
