LOCUS       CP035912                4166 bp    DNA     linear   BCT 19-FEB-2019
DEFINITION  Klebsiella pneumoniae strain BA4656 plasmid pCol440II.
ACCESSION   CP035912
VERSION     CP035912.1
DBLINK      BioProject: PRJNA521675
            BioSample: SAMN10908921
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Narasimman,V.,
            Neeravi,A.R. and Venkatesan,M.
  TITLE     Hypervirulent Multidrug resistant Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Narasimman,V.,
            Neeravi,A.R. and Venkatesan,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method :: Canu v. 1.7; Unicycler hybrid v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 58.0x
            Sequencing Technology :: IonTorrent; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/12/2019 14:22:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,254
            CDSs (total)                      :: 6,129
            Genes (coding)                    :: 5,530
            CDSs (with protein)               :: 5,530
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 599
            CDSs (without protein)            :: 599
            Pseudo Genes (ambiguous residues) :: 0 of 599
            Pseudo Genes (frameshifted)       :: 448 of 599
            Pseudo Genes (incomplete)         :: 160 of 599
            Pseudo Genes (internal stop)      :: 62 of 599
            Pseudo Genes (multiple problems)  :: 64 of 599
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BA4656"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCol440II"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2018"
                     /collected_by="Christian Medical College, Vellore, India"
     gene            208..693
                     /locus_tag="EXC92_31240"
     CDS             208..693
                     /locus_tag="EXC92_31240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QBE75949.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLNVLRMVGRTWLTITMVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEQKLSCVKVNPKAGAYGKEGNWMVLERK"
                     /name="mobilization protein"
     gene            697..909
                     /locus_tag="EXC92_31245"
     CDS             697..909
                     /locus_tag="EXC92_31245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QBE75950.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="mobilization protein"
     gene            complement(1628..1915)
                     /locus_tag="EXC92_31250"
     CDS             complement(1628..1915)
                     /locus_tag="EXC92_31250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QBE75951.1"
                     /translation="MFTACTVELAIYWYATTNNIPDETSHIINGRISQLNQFLLVLSPD
                     MANTKSPVRANMPTNTQEDVASRNNITFRINFRDTAIAIKPIRMSYSAVR"
                     /name="hypothetical protein"
     gene            complement(2656..2739)
                     /locus_tag="EXC92_31255"
     CDS             complement(2656..2739)
                     /locus_tag="EXC92_31255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QBE75954.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPDRHD"
                     /name="replication initiation protein"
     gene            complement(2764..3117)
                     /locus_tag="EXC92_31260"
                     /pseudo=""
     CDS             complement(2764..3117)
                     /locus_tag="EXC92_31260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001692121.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            3355..3696
                     /locus_tag="EXC92_31265"
     CDS             3355..3696
                     /locus_tag="EXC92_31265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QBE75952.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPAR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGGNDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3686..>4166
                     /locus_tag="EXC92_31270"
     CDS             3686..>4166
                     /locus_tag="EXC92_31270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="QBE75953.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELID
                     ASPYAKKYTSGVLSFAEAELPPGQREQIMASFERVLMPGLDKDQYSILWVEHTDKGRLE
                     LNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTVVNGRLGLHDPNAPENRRLLVTP"
                     /name="nuclease"
     Typing          3090..3268
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.409"
                     /coverage="83.0"
                     /evalue="3.53e-41"
     Typing          complement(2631..3144)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.054"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4..1025
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.774"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3660..4165
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.716"
                     /coverage="95.0"
                     /evalue="9.3e-95"
ORIGIN
        1 ccgcactgcc ggaaacgaag ctggaagccg ctcaggcgat tacacggggt ttgctggcct
       61 tagcctcatc cggggcgctt aaaacgcgcg aggacgtcac aggggcgctc acagcggcag
      121 gttttgaggt ggtccgcacc acaaagaaca gtatcagcat tgccgacccg gacggggggc
      181 gaaacatccg actgaaggga gccatttatg agcagtcttt tagcgctggc gaaggacttg
      241 gagcagcaat cgaaagcgca gcagcagagt accggcgaga tgctgaaagc cgcattcagc
      301 gagcacgaga agtctgtaaa agcggaactg agcgcaagcg cgaagagaat cagcgacgcc
      361 atcagcgccc acgagcaggg tatgaccgcg gcgatgcagt cgaaccggct gaacgtgctg
      421 cgtatggtcg gcaggacgtg gctgaccatc accatggtct cggtactgct gatcgccaca
      481 agcgggagca ttctgtggtg gcaggggcag cagatcaccg ggaattacca gactatccgg
      541 gcgcaggagc ggacgcaggc catgctgtca gagaagaacc acggcgtgca gctgtcgctc
      601 tgcggggagc agaaactgtc ctgcgtcaaa gtgaacccga aagcgggagc ttacgggaag
      661 gaagggaact ggatggtact ggagaggaaa tagcccatga cggaactgga aaaacagttg
      721 ctgaccgcat tagagcaact acatcaggac tactcgcaaa ggctggacga gtgggagagc
      781 gccttcgcgg aatggcagaa gatgtctggt cttatgcaac gggagaacgc ggcgctgaac
      841 gagcgcgtca cgcgcttgag tcagcaggtg gcgaacttga gcaggcagtt acagcgtttg
      901 agccagtagt gcagcggcat gaaatggcgg tggccgccga acgggcatat gagcagagac
      961 agcacgaaaa agagctggcg gaaacccgtt cccggcagcg gagctataac ggtccgacgc
     1021 tgggctgact gcgcgcatcc agtgacaaac cccaagaaga agggctcata aatttcccgc
     1081 taaatacggt cgtgatgttt ccatcacagc gtatacaacg gagggattat gagactgttt
     1141 aacgtgattg agacggtgct gaactgcctg tacatggtgc tggagatctg ggactggctg
     1201 gagaagcatt ttcctgtttt taaggcgttc atccggattc tggcctgaaa tcagactgta
     1261 tgcatctggc tgcatgacca tgcagccggg taaaatttat ctgtcacgaa cggggcggtg
     1321 tatcgggtgg tttgttgcct gttttatccc gtagccgccg gaaacgctct gagggcttct
     1381 cgggcggtgc tgataacgga tgttatggta aatctagctg ctagggcgat tcgcacgcca
     1441 tattttcagg tgtgatatcc ttatgcacca aggatgaaag gacaaaaaat aatccataag
     1501 gattcttttt atcgtctctg cttttttctt gggctccttc cgcttgtttc ctctctggta
     1561 ccatatgcgg actctactca ataaggatcg ttttgtgcct gctccaaaca attacggtgt
     1621 ggctggttca cctaacagcg ctatagctca tcctgatggg cttgattgct atggccgtgt
     1681 ccctgaaatt aattctaaag gtgatgttat tcctgctggc gacatcttct tgcgtgttgg
     1741 taggcatatt ggccctaacc ggggatttgg tgttcgccat atctgggctg agcacgagca
     1801 ggaactggtt aagctgggat atgcgaccgt taatgatgtg gctcgtttcg tccgggatat
     1861 tattcgtcgt ggcgtaccaa tatattgcga gttcaacagt acaagcggta aacatcggcc
     1921 tgcagtgcta aagtcgtcga taggcatcgt aattcttgag cccagagaag cgcctgaaac
     1981 tgagtctggt tggatctatg tcgttgtgac tgcgtacaca cgaaggacgg cgcacggtac
     2041 tttggttggt catattgaat aaaaaaatag ggcgctaaaa agcgcccttt atgaattcgt
     2101 tggtagtcgc agcatttcct tagcacatag ggttcagaaa ccgacgcttg tcgtaactac
     2161 ccggcagatg accagccatc tgactgcccg gcacagaagg cctattactg gttgatgccc
     2221 cctgcgaatg ctcttgcagt acaataatca taaacatcgt gatgtagatt tgcaaccaac
     2281 attttggttg caacctatat actaattttt ctcgataacg ccggaatcca ttgctgtgtc
     2341 tggctttctt gaagctcctg actatagata ccctatatcc taacagttat tctgctgtgg
     2401 atatcagaaa tgaacacttg agatcatcag tggctcgtcg tagtctcttg ctcagaaacg
     2461 aaaaaaccac cctggaaggt ggtttttccg aaggttagtt aatcctggca gattctctaa
     2521 ccgtggtaac agtcttgtgc gagacatgtc accaaatact gtcctttcag tgtagcctca
     2581 gttaggccgc cacttcaaga actctcgtta catctctcgc acatcctgct taccagtggc
     2641 cgttgccaat ggcgattagt cgtgtctatc cgggttggac tcaagacgat agttaccgga
     2701 caaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     2761 tgcctacccg gaactgagtg tcaggcgtgg aatgagataa acgcggccat aacagcggaa
     2821 tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagcca ccagggggaa
     2881 acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag cttcagattt
     2941 cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ctgcgctaca tcctttttct
     3001 gccccgcttg tcattccgta tgcttttgct cttatccgtg agcaggtaac gcccgccgca
     3061 gctgaacggc aggagcggaa gcgaccgagt gagcgaggag gcgtaatatc accgcaacct
     3121 tcatgttctc ccgtacgccc tgcagctgca tttcccctgt catatgaagc actttcctaa
     3181 agtgttacca cgtcgtaaca tattgagcca gtatacactc cgctagcgct acgtgactgg
     3241 ttcagggctg cgccccgaaa cccgctaaaa gccactgacg cgcctgcggc ttgtccaaca
     3301 ccgtgccgac cgggaaacat ttcccgcccg tcccggtgtt atcaggaggc tactctggca
     3361 gacaaacgca acaagatgct cacgatgtgg gtgacggaag acgaacaccg gcggctgctc
     3421 gagcggtgcg acggcaagca gctggcggcg tggatgcgtc agacctgcct cgatgagaag
     3481 ccggcgcgtg ccggcagact gccgtcgata tcgccggcgc tgctgcgcca gcttgccggt
     3541 atgggtaata acctcaacca gatcgcccgg aaagtgaatg ccggtggtgc cggtcatgac
     3601 cgggtgcaga tcgtggccgc cctgatggca atcgacgcgg gactggagcg gctgcggcat
     3661 gcggtgctgg aaaaaggggg gaatgatgat cgttaaattc catccccggg ggcgcggtgg
     3721 tggtgccggt ccggtggatt acctgctggg aaaagaccgg cagcgcgagg gggcgacagt
     3781 gctgcaggga aagccggagg aagtccggga gcttatcgat gcctcgccgt atgcgaaaaa
     3841 gtacacctcc ggcgtgctgt cgttcgcgga ggctgaactg ccgcccgggc agcgggagca
     3901 gatcatggcc agctttgagc gggttctgat gcccgggctc gataaagacc agtacagcat
     3961 tctgtgggtg gagcacaccg acaaggggcg gctggagctg aatttcctga tccccaacac
     4021 agagctgctg accgggaaac gcctgcagcc gtattacgat cgcgccgacc ggccgcgcat
     4081 cgatgcctgg cagaccgtgg tgaacggcag actggggctg catgatccaa acgccccgga
     4141 gaaccggcgg ttactggtca cgccgt
//
