LOCUS       CP035011                8398 bp    DNA     circular BCT 06-MAR-2019
DEFINITION  Shigella sonnei strain LC1477/18 plasmid pLC1477_18-3, complete
            sequence.
ACCESSION   CP035011
VERSION     CP035011.1
DBLINK      BioProject: PRJNA508357
            BioSample: SAMN10519737
KEYWORDS    .
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Clement,M., Principe,L., Bernasconi,O.J., Viaggi,V., Luzzaro,F. and
            Endimiani,A.
  TITLE     First case of ESBL-producing Shigella sonnei infection in Italy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8398)
  AUTHORS   Clement,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2019) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern, Bern 3001,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2018
            Assembly Method                   :: Canu v. 1.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/15/2019 13:18:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,039
            CDSs (total)                      :: 4,911
            Genes (coding)                    :: 4,119
            CDSs (with protein)               :: 4,119
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 792
            CDSs (without protein)            :: 792
            Pseudo Genes (ambiguous residues) :: 0 of 792
            Pseudo Genes (frameshifted)       :: 435 of 792
            Pseudo Genes (incomplete)         :: 335 of 792
            Pseudo Genes (internal stop)      :: 113 of 792
            Pseudo Genes (multiple problems)  :: 85 of 792
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="LC1477/18"
                     /isolation_source="Stools"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /plasmid="pLC1477_18-3"
                     /geo_loc_name="Italy: Lecco"
                     /lat_lon="45.852122 N 9.415050 E"
                     /collection_date="2018-08"
                     /collected_by="A. Manzoni Hospital, Lecco, Italy"
     gene            1..405
                     /locus_tag="EI553_25135"
     CDS             1..405
                     /locus_tag="EI553_25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /protein_id="QBJ61519.1"
                     /translation="MLHVAGEKRQHRRVSRICDTGDIPLPRSLTRYARSFGCGERYRLT
                     DGDGNFLEDTRKTLSKEEIRPRRSRFSIGSAPLTSIAKSDAQISGGETRQDYKDPRRFP
                     LVAPSCALLFLPFGLPVSFRCYGRGFSFHA"
                     /name="plasmid replication initiation protein"
     gene            447..605
                     /locus_tag="EI553_25140"
     CDS             447..605
                     /locus_tag="EI553_25140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QBJ61520.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="replication initiation protein"
     gene            1064..1879
                     /gene="sul2"
                     /locus_tag="EI553_25145"
     ARG             1065..1876
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1940..2743
                     /gene="aph(3'')-Ib"
                     /locus_tag="EI553_25150"
     ARG             1941..2740
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2743..3579
                     /locus_tag="EI553_25155"
     CDS             2743..3579
                     /locus_tag="EI553_25155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="QBJ61523.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            3483..3686
                     /locus_tag="EI553_25160"
                     /pseudo=""
     CDS             3483..3686
                     /locus_tag="EI553_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028401.1"
                     /note="incomplete; partial on complete genome; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein (MobL-like)"
                     /name="mobilization protein (MobL-like)"
     gene            3885..4127
                     /locus_tag="EI553_25165"
     CDS             3885..4127
                     /locus_tag="EI553_25165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="QBJ61524.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="relaxase"
     gene            complement(4159..4836)
                     /locus_tag="EI553_25170"
     CDS             complement(4159..4836)
                     /locus_tag="EI553_25170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="QBJ61525.1"
                     /translation="MFISDKVSSMTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGV
                     QQPALYWHFRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRD
                     GARIHAGTRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSD
                     AGERGGTVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRK
                     GDD"
                     /name="TetR family transcriptional regulator"
     ARG             4916..6447
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="97.19"
                     /coverage="120.28"
     ARG             4916..6081
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="97.94"
                     /coverage="97.49"
     gene            complement(6079..6267)
                     /locus_tag="EI553_25180"
                     /pseudo=""
     CDS             complement(6079..6267)
                     /locus_tag="EI553_25180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial on complete genome; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(6311..6700)
                     /locus_tag="EI553_25185"
     CDS             complement(6311..6700)
                     /locus_tag="EI553_25185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QBJ61526.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            6870..7358
                     /locus_tag="EI553_25190"
     CDS             6870..7358
                     /locus_tag="EI553_25190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QBJ61527.1"
                     /translation="MFCCYNTVHTGDTVTFMAWHTVQETLKLTGKSRSQLYRDMAKGLV
                     SYRTGKNDRREFETSELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDA
                     LQQTVTLMLEDKMAREAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="entry exclusion protein 1"
     gene            7504..7734
                     /locus_tag="EI553_25195"
     CDS             7504..7734
                     /locus_tag="EI553_25195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QBJ61528.1"
                     /translation="MHEYAHKCIARKFTPFSRGKSCFRRSSHPKTVNGDAIYCVAVKRC
                     RRSRKTRCQHVQEGGDVQMAQRMKVTYELKE"
                     /name="hypothetical protein"
     ARG             2744..3576
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(2..85)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.75e-36"
     Typing          complement(8310..8398)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.889"
                     /coverage="100.0"
                     /evalue="2.12e-37"
     Typing          9..614
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtgcttcatg tggcaggaga aaaaaggcag caccggcgcg tcagcagaat atgcgataca
       61 ggagatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag
      121 cggtaccggc ttacggacgg ggacggaaat ttcctggaag ataccaggaa gacacttagc
      181 aaagaagaga taaggccacg gcgaagccgt ttttccatag gctccgcccc cctgacaagc
      241 atcgcaaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatccc
      301 aggcgtttcc ccctggtagc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
      361 gtgtcattcc gctgttatgg ccgcggtttc tcattccacg cctgacactc agttccgtga
      421 aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg
      481 ccttgtccgg taactatcaa cttgagtcca acccggaaag acacgacaaa acgccactgg
      541 cagcagccat tagtaacagg agtgcacgag aagatagatt aagaggtctt gaagtagagg
      601 cctgactgcg gctacactga aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta
      661 ccgggttaag cagttcccca actgacttaa ccttcgaaca aaccacctcc cgggcggttt
      721 tttcgtttac agagcaagag attacgattc attgaaaagg atctcaagaa gatcctttgt
      781 agattactat caattaatct tccgaacatg acaccagtca ctgttgatta cggttaaacc
      841 gctccgtaac aggtttccct cgtattggtg acttgcagca ccaaagaagc aaaaaagata
      901 aatatatcta cttattgaga gatgatatat ttatttccaa aatgctacag atacatcatc
      961 atgaaacgga tcaccgcaaa caggttactc gcctggggat tccctttcga cccgagcatc
     1021 cgtatgagac tcatgctcga ttattattat tatagaagcc cccatgaata aatcgctcat
     1081 cattttcggc atcgtcaaca taacctcgga cagtttctcc gatggaggcc ggtatctggc
     1141 gccagacgca gccattgcgc aggcgcgtaa gctgatggcc gagggggcag atgtgatcga
     1201 cctcggtccg gcatccagca atcccgacgc cgcgcctgtt tcgtccgaca cagaaatcgc
     1261 gcgtatcgcg ccggtgctgg acgcgctcaa ggcagatggc attcccgtct cgctcgacag
     1321 ttatcaaccc gcgacgcaag cctatgcctt gtcgcgtggt gtggcctatc tcaatgatat
     1381 tcgcggtttt ccagacgctg cgttctatcc gcaattggcg aaatcatctg ccaaactcgt
     1441 cgttatgcat tcggtgcaag acgggcaggc agatcggcgc gaggcacccg ctggcgacat
     1501 catggatcac attgcggcgt tctttgacgc gcgcatcgcg gcgctgacgg gtgccggtat
     1561 caaacgcaac cgccttgtcc ttgatcccgg catggggttt tttctggggg ctgctcccga
     1621 aacctcgctc tcggtgctgg cgcggttcga tgaattgcgg ctgcgcttcg atttgccggt
     1681 gcttctgtct gtttcgcgca aatcctttct gcgcgcgctc acaggccgtg gtccggggga
     1741 tgtcggggcc gcgacactcg ctgcagagct tgccgccgcc gcaggtggag ctgacttcat
     1801 ccgcacacac gagccgcgcc ccttgcgcga cgggctggcg gtattggcgg cgctgaaaga
     1861 aaccgcaaga attcgttaac tgcacattcg ggatatttct ctatattcgc gcttcatcag
     1921 aaaactgaag gaacctccat tgaatcgaac taatattttt tttggtgaat cgcattctga
     1981 ctggttgcct gtcagaggcg gagaatctgg tgattttgtt tttcgacgtg gtgacgggca
     2041 tgccttcgcg aaaatcgcac ctgcttcccg ccgcggtgag ctcgctggag agcgtgaccg
     2101 cctcatttgg ctcaaaggtc gaggtgtggc ttgccccgag gtcatcaact ggcaggagga
     2161 acaggagggt gcatgcttgg tgataacggc aattccggga gtaccggcgg ctgatctgtc
     2221 tggagcggat ttgctcaaag cgtggccgtc aatggggcag caacttggcg ctgttcacag
     2281 cctatcggtt gatcaatgtc cgtttgagcg caggctgtcg cgaatgttcg gacgcgccgt
     2341 tgatgtggtg tcccgcaatg ccgtcaatcc cgacttctta ccggacgagg acaagagtac
     2401 gccgcagctc gatcttttgg ctcgtgtcga acgagagcta ccggtgcggc tcgaccaaga
     2461 gcgcaccgat atggttgttt gccatggtga tccctgcatg ccgaacttca tggtggaccc
     2521 taaaactctt caatgcacgg gtctgatcga ccttgggcgg ctcggaacag cagatcgcta
     2581 tgccgatttg gcactcatga ttgctaacgc cgaagagaac tgggcagcgc cagatgaagc
     2641 agagcgcgcc ttcgctgtcc tattcaatgt attggggatc gaagcccccg accgcgaacg
     2701 ccttgccttc tatctgcgat tggaccctct gacttggggt tgatgttcat gccgcctgtt
     2761 tttcctgctc attggcacgt ttcgcaacct gttctcattg cggacacctt ttccagcctc
     2821 gtttggaaag tttcattgcc agacgggact cctgcaatcg tcaagggatt gaaacctata
     2881 gaagacattg ctgatgaact gcgcggggcc gactatctgg tatggcgcaa tgggagggga
     2941 gcagtccggt tgctcggtcg tgagaacaat ctgatgttgc tcgaatatgc cggggagcga
     3001 atgctctctc acatcgttgc cgagcacggc gactaccagg cgaccgaaat tgcagcggaa
     3061 ctaatggcga agctgtatgc cgcatctgag gaacccctgc cttctgccct tctcccgatc
     3121 cgggatcgct ttgcagcttt gtttcagcgg gcgcgcgatg atcaaaacgc aggttgtcaa
     3181 actgactacg tccacgcggc gattatagcc gatcaaatga tgagcaatgc ctcggaactg
     3241 cgtgggctac atggcgatct gcatcatgaa aacatcatgt tctccagtcg cggctggctg
     3301 gtgatagatc ccgtcggtct ggtcggtgaa gtgggctttg gcgccgccaa tatgttctac
     3361 gatccggctg acagagacga cctttgtctc gatcctagac gcattgcaca gatggcggac
     3421 gcattctctc gtgcgctgga cgtcgatccg cgtcgcctgc tcgaccaggc gtacgcttat
     3481 gggtgccttt ccgcagcttg gaacgcggat ggagaagagg agcaacgcga tctagctatc
     3541 gcggccgcga tcaagcaggt gcgacagacg tcatactaga tatcaagcga cttctcctat
     3601 cccctgggaa cacatcaatc tcaccggaga atatcgctgg ccaaagcctt agcgtaggat
     3661 tccgcccctt cccgcaaacg acccctggaa gccccgtagc gacgcggaga ggggcgagac
     3721 aagccaaggg cgcaggctcg atgcgcagca cgacatagcc ggttctcgca aggacgagaa
     3781 tttccctgcg gtgcccctca agtgtcaatg aaagtttcca acgcgagcca ttcgcgagag
     3841 ccttgagtcc acgctagatc tatctcatct gcgcaaggca gaacgtgaag acggccgccc
     3901 tggacctcgc ccgcgagcgc caggcgcacg aggccggcgc gcggacccgc gccacggccc
     3961 acgagcggac gccgcagcag gagcgccaga aggccgccag agaggccgag cgcggccgtg
     4021 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     4081 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     4141 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     4201 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     4261 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     4321 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     4381 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     4441 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     4501 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     4561 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     4621 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     4681 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     4741 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     4801 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     4861 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     4921 cccaacagac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     4981 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     5041 gcccactatg gcattctgct ggcgctgtat gcgttgatgc aatttgcctg cgcacctgtg
     5101 ctgggcgcgc tgtcggatcg tttcgggcgg cggccggtct tgctcgtctc gctggccggc
     5161 gctgctgtcg actacgccat catggcgacg gcgcctttcc tttgggttct ctatatcggg
     5221 cggatcgtgg ccggcatcac cggggcgact ggggcggtag ccggcgctta tattgccgat
     5281 atcactgatg gcgatgagcg cgcgcggcac ttcggcttca tgagcgcctg tttcgggttc
     5341 gggatggtcg cgggacctgt gctcggtggg ctgatgggcg gtttctcccc ccacgctccg
     5401 ttcttcgccg cggcagcctt gaacggcctc aatttcctga cgggctgttt ccttttgccg
     5461 gagtcgcaca aaggcgaacg ccggccgtta cgccgggagg ctctcaaccc gctcgcttcg
     5521 ttccggtggg cccggggcat gaccgtcgtc gccgccctga tggcggtctt cttcatcatg
     5581 caacttgtcg gacaggtgcc ggccgcgctt tgggtcattt tcggcgagga tcgctttcac
     5641 tgggacgcga ccacgatcgg catttcgctt gccgcatttg gcattctgca ttcactcgcc
     5701 caggcaatga tcaccggccc tgtagccgcc cggctcggcg aaaggcgggc actcatgctc
     5761 ggaatgattg ccgacggcac aggctacatc ctgcttgcct tcgcgacacg gggatggatg
     5821 gcgttcccga tcatggtcct gcttgcttcg ggtggcatcg gaatgccggc gctgcaagca
     5881 atgttgtcca ggcaggtgga tgaggaacgt caggggcagc tgcaaggctc actggcggcg
     5941 ctcaccagcc tgacctcgat cgtcggaccc ctcctcttca cggcgatcta tgcggcttct
     6001 ataacaacgt ggaacgggtg ggcatggatt gcaggcgctg ccctctctgc tctgcctgcc
     6061 ggcgctgcgt cgcgggcttt ggagaaattc ttcaaattcc cgttgcacat agcccggcaa
     6121 ttcctttccc tgctctgcca taagcgcagc gaatgccggg taatactcgt caacgatctg
     6181 atagagaagg gtttgctcgg gtcggtggct ctggtaacga ccagtatccc gatcccggct
     6241 ggccgtcctg gccgccacat gaggcatgtt ccgcgtcctt gcaatactgt gtttacatac
     6301 agtctatcgc ttagcggaaa gttcttttac cctcagccga aatgcctgcc gttgctagac
     6361 attgccagcc agtgcccgtc actcccactc ttacattgtt cgcagtatct aggtacatgt
     6421 tattgcgtgc accttcatga tgtataatgt tattaatgac agcagaaaga tcgacagaat
     6481 taatattctt cgtacctggg acagttatcg ggtgtcggcc agtgatgaga ccaaaggatg
     6541 cagtataaga tgaaccacta ctattatcat ggccaccatt accaccaccg ttaaaactac
     6601 ctcccgaact tccagagccg ctatgaccat cattgtaggg gtcatttggt cccatgtgac
     6661 caaaaccacc gacagtaccg ctatttaact ctgtactcat aaaattatcc tctttgactt
     6721 ttaaaaacag caaaaactgt atttataaca atgtgtttat atacagtatt cgaaatacta
     6781 cgcccgtcaa aaccacaccg tcaacaggtt ttttgtgtat ttttcacgcc tcacgggcag
     6841 cctcagacct tttgtgttaa aacagtaata tgttctgctg ttacaatacg gtacacacag
     6901 gagacactgt cacttttatg gcatggcaca cagttcaaga gacactgaaa ttaacaggaa
     6961 agtcgcgctc gcaattatat cgtgacatgg ctaaaggcct tgtcagctat cgcacaggaa
     7021 aaaatgatcg ccgggagttc gagaccagtg aactgattcg ggcctatggc gagctgcgca
     7081 cttctgagac accaaaaagg cacaaggagg gacactccaa atctgtcgtt agccaggcgg
     7141 aaaatgctca gctagacgtc attcagcaga aactggacgc tctgcagcag actgttaccc
     7201 taatgcttga agacaagatg gcgcgggaag cagaaaaccg cctgcgaaat gaggagaggg
     7261 aacaactaca ggccgaaatt agccgtctgg ctgacgcgct ggcacaggaa aaaaagcggg
     7321 gattctggtc gcggctgttt cgctccggtt cagagtaaca gtgcaggtga ctctgattta
     7381 ccataacatc tgttatcagc accgcctgag aggccctcag ggcgtttctg acgggaaacc
     7441 ggtaaaacag gcaacaaacc acccgaaaac ccgccagaaa cgcgcaggct ctgtttttac
     7501 ggcatgcatg agtatgcgca caagtgcata gcgcggaaat tcacaccatt cagccggggt
     7561 aagtcctgtt tccggcgttc ttcccatccg aaaacggtga acggggacgc aatttattgc
     7621 gtggccgtga agcgttgccg gcgcagccgg aaaacccgct gtcagcacgt tcaggaaggg
     7681 ggtgatgtac aaatggcaca gaggatgaaa gtgacgtatg agctgaagga atgatgtaaa
     7741 agtgatgtat aagtgatgta aaagtgatgt ataagtgatg taaaagtgat gtatgaactg
     7801 aaggagcgat gtaattctgt agtcaggaag aataacccgt tcatgcaggc atgaacgata
     7861 cagtgaggaa cgcttttttt gtcacgatct ccccttaacc tgcgtaaatc acccgccatg
     7921 gttgattatt tttcaacctc aaaatggtca tacacctaac gcagccgcga ttttcatcgc
     7981 aactgcatga attaaaagaa aaaaaaggaa tatttcgttt tcacgagtaa tacccgggta
     8041 ttaaaaatcc actgtaatta catgaaaata aagaaataaa aggtaatacc ggagtattac
     8101 cacgtacgcg ggcaagcgaa aaagctataa cgcttcccgg ccgacaggcg acccaaaatc
     8161 cacaggggca tgcaggttct cttcagtaac ctgcgccatt aaatcaaaaa ggtcatattc
     8221 gccgggaagc tgcgtatcag tcgaatccag ttgttcatca tattcagatg gcatggggtg
     8281 atatccagta gctgagaggt tgttagcggg tttcggggcg cagccctgaa ccagtcatgt
     8341 agcgctagcg gagtgtatac tggcttacta tgttgccacg attgcgaaaa tcagtgaa
//
