LOCUS       CP031812                3354 bp    DNA     circular BCT 30-AUG-2018
DEFINITION  Klebsiella pneumoniae strain INF014-sc-2279884 plasmid unnamed2,
            complete sequence.
ACCESSION   CP031812
VERSION     CP031812.1
DBLINK      BioProject: PRJNA486877
            BioSample: SAMEA3356957
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Wyres,K.L., Wick,R., Judd,L.M., Froumine,R., Tokolyi,A., Gorrie,C.,
            Lam,M.C., Duchene,S., Jenney,A. and Holt,K.E.
  TITLE     Distinct evolutionary dynamics of horizontal gene transfer in drug
            resistant and virulent clones of Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Wyres,K.L., Wick,R., Judd,L.M., Froumine,R., Tokolyi,A., Gorrie,C.,
            Lam,M.C., Duchene,S., Jenney,A. and Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2018) Department of Biochemistry and Molecular
            Biology; Bio21 Molecular Science and Biotechnology Institute,
            University of Melbourne, University of Melbourne, Parkville, VIC
            3010, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 108x
            Sequencing Technology :: Nanopore minION; Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2018 13:47:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.6
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,524
            CDS (total)                       :: 5,402
            Genes (coding)                    :: 5,250
            CDS (coding)                      :: 5,250
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 87 of 152
            Pseudo Genes (incomplete)         :: 63 of 152
            Pseudo Genes (internal stop)      :: 41 of 152
            Pseudo Genes (multiple problems)  :: 36 of 152
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF014-sc-2279884"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
     gene            complement(95..517)
                     /locus_tag="D0897_27560"
     CDS             complement(95..517)
                     /locus_tag="D0897_27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="AXS38719.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     VSELIRVFGSLDGVTDTVKYDVKSDAISTEVNTIGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHDPSSPAHSPWWKFWKKS"
                     /name="DNA-binding protein"
     gene            complement(517..708)
                     /locus_tag="D0897_27565"
     CDS             complement(517..708)
                     /locus_tag="D0897_27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="AXS38720.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDSLQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            665..1081
                     /locus_tag="D0897_27570"
     CDS             665..1081
                     /locus_tag="D0897_27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014343523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /protein_id="AXS38721.1"
                     /translation="MNLAMFSAVFCCFVIKGLRASVLSVKPVSFPLPRSLSRYAPVVRL
                     RRAAGACIEKLEKTERSKGEKKERPRRCRFSIGSAPLTSIAETDAQISGGETRQDYKDT
                     RRFPLAAPSCALLFLPFGLPVSFRCYGRGFSFHA"
                     /name="plasmid replication initiation protein"
     gene            1123..1254
                     /locus_tag="D0897_27575"
     CDS             1123..1254
                     /locus_tag="D0897_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="AXS38722.1"
                     /translation="MHEPPVQSDCCAFSGNFQLDSKPKRHDKAPLAAAIGNGVAEQS"
                     /name="replication initiation protein"
     gene            complement(1444..1845)
                     /locus_tag="D0897_27580"
     CDS             complement(1444..1845)
                     /locus_tag="D0897_27580"
                     /inference="COORDINATES: protein motif:HMM:PF05168.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="AXS38723.1"
                     /translation="MISSKEMLDFARGSYSKFDAAGYICLSEEVEYRTCARSAYYALYH
                     YLKSIADKLPGSYEDVSSHEKVIRKLLNSGDPKLMDLAQKMISTRKTRVRADYHIDKNF
                     GKSEAYKILRIVEKIFKDAELSEGEVLDS"
                     /name="HEPN domain-containing protein"
     gene            complement(1842..2633)
                     /locus_tag="D0897_27585"
     CDS             complement(1842..2633)
                     /locus_tag="D0897_27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014343525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AXS38724.1"
                     /translation="MVSISPKKAEDEALDILNRVIVEKRNVSEFENVRIINCLDSLRRA
                     RSSSYDEISACYYAQIADIDNLLVSVSNVFSSDCEDSFFNIIYALNNAMRYREIYKYVK
                     DLDLDYNIRDFLESAIKASAFCVDKEMVDKFTTSYTNAFHDESAVDFLNHISEINSFFL
                     NHESIKKEMSNYMLDCFDVFSRKSLIKTKEIDGTYGYSHHVFNDEGYEFLAFSFILRGD
                     EIDMDMLIDIEDEFTYEISRIKYDPIIKTKISFNFELGDLS"
                     /name="hypothetical protein"
     gene            complement(2753..2953)
                     /locus_tag="D0897_27590"
     CDS             complement(2753..2953)
                     /locus_tag="D0897_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AXS38726.1"
                     /translation="MNCVPVHRFRRRRTPETGLPLAEWCQFPRYALARILMHAVKTEPA
                     RFWRVFGWFVACFTGFPSETP"
                     /name="hypothetical protein"
     gene            2933..3169
                     /locus_tag="D0897_27595"
     CDS             2933..3169
                     /locus_tag="D0897_27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AXS38725.1"
                     /translation="MNGDAIYCVAVKRCRRSRKTGFQHVQESGSPIAGNDVMASRRTTH
                     HTIHRRLFTRVVPRVASSKKQPNGESCLESGRY"
                     /name="hypothetical protein"
     Typing          867..1231
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.278"
                     /coverage="85.0"
                     /evalue="4.93e-157"
ORIGIN
        1 ttagtggtga ggagcgttca gagatctgac gatttttggg aaggttcagg aactccccgg
       61 gatcgccgga gagcaaaact gaaacatggc aggatcagga ttttttccag aatttccacc
      121 acggagaatg agccggtgaa gaggggtcgt gcttgtgctc caaaagctgc atagcctggc
      181 gaagggaatc aatatgctca tcgcgtgctt taacagcccc tttaagacca tcgttttcag
      241 cttcaagtaa agcgattcta tgttgtaaat catgtaaacc aatggtgtta acctctgtag
      301 aaattgcgtc actttttaca tcatatttta cagtatcagt tacaccatca agagaaccaa
      361 acaccctaat aagctcggat acatcaataa ctttaatatc cttcccgtgc tttacaacag
      421 aggtataact taacttccca ctgttaatca tcttgtaaat agtgggacga ctaaccttaa
      481 caagccgtgc agcttcacta atagacactc tggccatcag ttaccatcct gtaatgaatc
      541 cagtctcgag gagagtgtac ggaaaagatg ctctgcatca tcgtggagtt tctcacaaag
      601 gtcggcctca gcatcaagat caagctcgtt aagcttctcc agcaggagca gagactgatt
      661 ctggatgaat ttagccatgt tcagcgccgt tttctgttgt ttcgtcataa agggcctcag
      721 agcgtcagtg ctcagtgtaa aacctgtctc ttttccgctt cctcgctcac tcagtcgcta
      781 cgctccggtc gttcgactgc ggcgagcggc aggagcatgc atagaaaagc tcgagaaaac
      841 cgagcggtca aagggggaaa aaaaggaaag gccacggcgt tgccgttttt ccataggctc
      901 cgcccccctg acaagcatcg cagaaacgga cgctcaaatc agtggtggcg aaacccgaca
      961 ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct
     1021 gcctttcggt ttgccggtgt cattccgctg ttatggccgc ggtttctcat tccacgcctg
     1081 acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt
     1141 cagtccgact gctgcgcctt ttccggtaac tttcaacttg attccaaacc gaaaagacac
     1201 gacaaagcgc cactggcagc agccattggt aacggtgtcg cagagcagag ttaacgagag
     1261 ttcttgaagt ggctgcctaa aaacggctac actggaagga cagtatttgg tacctgtgct
     1321 ctgcgaaagc cagttacctc ggtcaaaaga gttggtagct ctgaagacct tgaaaaacca
     1381 ccgttggtag cggtggtttt tctctatctg gaggttacta cctcctcttt attcccgtca
     1441 agctcatgaa tccagaactt ccccttcgga aagttcagca tccttgaaga tcttttcaac
     1501 aatacgaagt attttgtatg cctctgattt gccgaaattt ttatctatat gatagtcagc
     1561 tcgaactctc gtcttccttg tggaaatcat cttctgagct aaatccataa gttttgggtc
     1621 gccagaatta agaagctttc gaatcacttt ttcatgtgac gaaacatctt cgtagctgcc
     1681 aggcaacttg tcagcaatac tcttcaaata atgatacaac gcataataag cggaacgagc
     1741 gcaagtccta tactctacct cttcactcag gcagatataa ccagccgcat caaattttga
     1801 ataagagcct ctcgcaaaat ctagcatttc ctttgaagag atcatgacaa atctcccaat
     1861 tcaaaattaa atgaaatttt tgtttttata attggatcat attttattct agatatttca
     1921 tatgtaaact catcttcgat atcaataagc atatccatgt caatttcatc acctctcaat
     1981 ataaagctga aagctaaaaa ctcgtaaccc tcatcattga agacgtgatg tgaataacca
     2041 taagttccgt caatttcttt ggtttttata agagacttcc ttgaaaaaac atcaaagcaa
     2101 tcaagcatat aattagacat ttcttttttt atgctttcat gattcaaaaa gaaggagttt
     2161 atttcactta tatgatttaa aaaatccaca gcactttcat catgaaaagc atttgtgtaa
     2221 gatgtggtga atttatcaac catctcttta tcaacacaaa aagcagacgc ctttattgct
     2281 gactctaaaa aatctcttat attataatca aggtcaagat cttttacata tttatagatc
     2341 tctctatatc tcattgcatt atttaatgcg taaatgatat taaagaaaga atcctcgcaa
     2401 tctgaagaga aaacgttaga aacactaacc aatagattat ctatatctgc gatctgtgcg
     2461 taatagcaag cagagatttc atcgtaagat gaacttcttg cccttcttaa agagtccaag
     2521 caatttataa ttcgtacatt ctcaaattct gatacgtttc ttttttcaac tataactcta
     2581 ttcaaaatat ccaaagcttc atcttctgct tttttaggag atatagatac catgaaattc
     2641 actcttttaa tttactttta aatcaggata gcattttatc ggtacaaaaa aaattatctt
     2701 taatacaacc tatttaccat aacatccgtt atcagcaccg cctgagaggc cctcagggcg
     2761 tttctgacgg gaaaccggta aaacaggcaa caaaccaccc gaaaacccgc cagaaacgcg
     2821 caggctctgt ttttacggca tgcatgagta tgcgcgcaag tgcatagcgc ggaaattgac
     2881 accattcagc caggggaagt cctgtttccg gcgttcttct cctccgaaaa cggtgaacgg
     2941 ggacgcaatt tattgcgtgg ccgtgaagcg ttgccggcgc agccggaaaa ccggttttca
     3001 gcacgttcag gaatcgggtt cgccgatagc cggtaatgac gtgatggcaa gtcgccggac
     3061 gactcaccac acgattcacc ggcgactctt cactagagtc gtacccagag tcgcctcgtc
     3121 taaaaaacaa ccaaacgggg agtcgtgtct agagtcgggt cgttattaac ttattgtttt
     3181 tatagtcctg agtcgttacc gactctatga cgactcgccg gggactctat gacgagtcat
     3241 catagagtcg gatctatata aataaaattt aaaaaacgaa atttcaggaa agtaaaccac
     3301 tgatattaaa tacaaatctg caaaatttta attttgcgta gtgtgtgggt attt
//
