LOCUS       CP031737                6141 bp    DNA     circular BCT 27-AUG-2018
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain M5 plasmid pKPM503,
            complete sequence.
ACCESSION   CP031737
VERSION     CP031737.1
DBLINK      BioProject: PRJNA486427
            BioSample: SAMN09845872
KEYWORDS    .
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Lee,H. and Ko,K.S.
  TITLE     Klebsiella pneumoniae M5 coproducing NDM-1 and OXA-232
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Lee,H. and Ko,K.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2018) Molecular Cell Biology, Sungkyunkwan
            University, 2066 Seobu-ro, Suwon 16419, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-MAY-2018
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 344.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/21/2018 09:24:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.6
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,915
            CDS (total)                       :: 5,793
            Genes (coding)                    :: 5,588
            CDS (coding)                      :: 5,588
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 101 of 205
            Pseudo Genes (incomplete)         :: 100 of 205
            Pseudo Genes (internal stop)      :: 48 of 205
            Pseudo Genes (multiple problems)  :: 41 of 205
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="M5"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pKPM503"
                     /geo_loc_name="South Korea: Seoul"
                     /lat_lon="37.498095 N 127.07610 E"
                     /collection_date="10-Dec-2015"
                     /collected_by="Human host"
     gene            join(6058..6141,1..714)
                     /locus_tag="D0S46_29535"
     CDS             join(6058..6141,1..714)
                     /locus_tag="D0S46_29535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="AXQ46134.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            complement(1121..1273)
                     /locus_tag="D0S46_29540"
                     /pseudo=""
     CDS             complement(1121..1273)
                     /locus_tag="D0S46_29540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial on complete genome; missing
                     start; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            1649..2596
                     /locus_tag="D0S46_29545"
     CDS             1649..2596
                     /locus_tag="D0S46_29545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicase"
                     /protein_id="AXQ46135.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replicase"
     gene            3004..3396
                     /locus_tag="D0S46_29550"
     CDS             3004..3396
                     /locus_tag="D0S46_29550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AXQ46136.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="hypothetical protein"
     gene            3378..3716
                     /gene="mobC"
                     /locus_tag="D0S46_29555"
     CDS             3378..3716
                     /gene="mobC"
                     /locus_tag="D0S46_29555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005322038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="AXQ46137.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="mobC"
     gene            3697..4407
                     /locus_tag="D0S46_29560"
                     /pseudo=""
     CDS             3697..4407
                     /locus_tag="D0S46_29560"
                     /inference="COORDINATES: protein motif:HMM:PF03432.12"
                     /note="incomplete; partial on complete genome; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization relaxase"
                     /name="mobilization relaxase"
     gene            4385..4822
                     /locus_tag="D0S46_29565"
     CDS             4385..4822
                     /locus_tag="D0S46_29565"
                     /inference="COORDINATES: protein motif:HMM:PF04837.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobB protein"
                     /protein_id="AXQ46138.1"
                     /translation="MSKILDMAKDFEQSSKQQANAIEKRLTEEFTRHEKAISGALKSSE
                     EKMSAAIAEHSQKMGWLLLKTWIWMLVSIVLFLSATSGILWYQGELIAERQATLQALAE
                     KGGKLKLTQCGKKLEHLCVQISDPNPTVWGKDTYWIVPKGY"
                     /name="MobB protein"
     gene            4823..5095
                     /locus_tag="D0S46_29570"
     CDS             4823..5095
                     /locus_tag="D0S46_29570"
                     /inference="COORDINATES: protein motif:HMM:PF04899.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MobD"
                     /protein_id="AXQ46139.1"
                     /translation="MTGLERELLCAFEALQSAYEQQHKAWQESYANLASMYESTKQEAE
                     ISRAAAERLTRQVNGLTQQVDGLSKQLEGLTGLVSKLQKKGNTGS"
                     /name="protein MobD"
     gene            complement(5433..5714)
                     /locus_tag="D0S46_29575"
     CDS             complement(5433..5714)
                     /locus_tag="D0S46_29575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AXQ46140.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     ARG             2..714
                     /name="OXA-405"
                     /gene="OXA-405"
                     /gene_name="OXA beta-lactamase; OXA-48-like beta-lactamase"
                     /tool="rgi"
                     /drug_class="carbapenem; penam"
                     /identity="96.62"
                     /coverage="90.8"
     ARG             6059..6141
                     /name="OXA-788"
                     /gene="OXA-788"
                     /gene_name="OXA beta-lactamase; OXA-48-like beta-lactamase"
                     /tool="rgi"
                     /drug_class="carbapenem; penam"
                     /identity="100.0"
                     /coverage="10.73"
     Typing          1662..2583
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          3698..5148
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaagttgga atgctcactt tactgaacat aaatcacagg gcgtagttgt gctctggaat
       61 gagaataagc agcaaggatt taccaataat cttaaacggg cgaaccaagc atttttaccc
      121 gcatctacct ttaaaattcc caatagcttg atcgccctcg atttgggcgt ggttaaggat
      181 gaacaccaag tctttaagtg ggatggacag acgcgtgata tcgccgcttg gaatcgtgac
      241 catgacttaa ttaccgcgat gaagtactca gttgtgcctg tttatcaaga atttgcccgc
      301 caaattggtg aggcacgtat gagtaaaatg ctgcacgcct tcgattatgg caatgaggat
      361 atctcgggca atgtagacag tttttggctc gatggtggta ttcgcatttc ggctacccag
      421 caaatcgctt ttttacgcaa gctgtatcac aacaagctgc acgtttctga gcgtagtcag
      481 cgcatcgtga aacaagccat gctgaccgaa gccaatggcg actatattat tcgggctaaa
      541 acgggatact cgactagtat cgaacctaag attggctggt gggttggttg ggttgaactt
      601 gatgataatg tgtggttttt tgcgatgaat atggatatgc ccacatcgga tggtttaggg
      661 ctgcgccaag ccatcacaaa agaagtgctc aaacaggaga aaattattcc ctagaagtgg
      721 ttagcgcgta tttgtgtgaa atagccgtca tataagctgt aaagatatat ggacaaaatg
      781 ctgatgctcg atgcgcttgt agttaatggc tcatacttaa tcggtcaggc ctaatcgact
      841 taagctccgc gcgttaactt tcagcctgag cattagcatc acattcctaa acgtaaaaag
      901 cctagccaat ttgctaggct tttttgttat ctcaatggcc atggtcactc aacatggggg
      961 tgtgaataaa ggagtggcga tgtcagtaaa ggtgaatgcc gctgcgataa agtgcctagc
     1021 ccatcaagga ttgtaggctt tcttggctgc taaagcgttt gacgaaaaag tccaagaagg
     1081 cgctgatatt ggttctccat cattttcatc taccggttta ctataggtca accaggccgt
     1141 ccttgccggc gcttgggacg cagacgatgg catcaaatgc ctcgctcaaa ttcgtttcta
     1201 cagaggcgct ttgggaccgc attcgctttg ctttcatggg ggcatctgtc aatgtcagac
     1261 atgaatcctc cgatataggt tgatttttga tctcttcaac acttggtgtt gtccgactag
     1321 agagacttat atggatcagt attcttgcac tgacaatggt gccactcaat cccaaggatc
     1381 gacctagtta gaggcgtaga tcaagcaatt caaaacagcg cagaagcaga aggaggtgga
     1441 aatatgttcc agggtgaaag gttacatgca actaacgttg ctctctagga tgtgcctgct
     1501 gtaacgtatt gtccagaaaa gacaaccccc cgttatcgct tgcatcttgg cggagctgcg
     1561 aaaaacgggg gccgtgacga atcacaagag aagtctatca catggatcat gccaaacaac
     1621 cccacgccgc caataagcgc ggtggtgctt gagaaagtgc gcacagcttg agctgtttcg
     1681 ggagcgcctg ccccggaaac cctactattc cgacgagctt acaacgggcc tacggattgc
     1741 ggatgttgcc cgcgcccttg gtgcgcgtta catccagccg aacgggccaa cgcaccgcca
     1801 ctggatcgtt tttgacgtag accatgcagc cgctacgtta tcttgggatg acgtaggcgc
     1861 tccagcccct aacattaccg tcacgaacaa ggccaacggc cacgcgcatc tgatctacgg
     1921 actggatacg ccgatccgca ctgccccgga tggaaatgca gcccctctgc ggtatgccgc
     1981 cgccatcgag gccgcgctcc gggagaaatt aggggcggat atgggctact ccgggctgat
     2041 ctgcaagaac cccctgcatg agcactggct ggtgcaggta tgggaacccc ggctctacga
     2101 tttagcctgg ctatccgatt acctcgatct gagctcctac aatggccgca aatcgcttcc
     2161 tgagtacggg cttgggcgaa actgtaccct gttcgagaaa acgcgcctgt gggcctataa
     2221 agcgatccgg cagggctggc cggattaccc cgaatggttg gccgcttgtg ttgaccgggc
     2281 atcaggatat aacgcccagt tcgagcagcc gctccccgca aacgaagtcc ggcacacggc
     2341 caagtccatt gccaaatgga cgcaccagca cctaagcccc gcagggttca gagaggagca
     2401 ggcccggaga ggtgcgaagg gcggcaaggt atccaaggga gggggaaggc cgagcaatag
     2461 cggtaaggac aagtccgatc tattgccgga ggttctgcgg cttaaggctc aggggtacac
     2521 caaccgcgat atagcggacg atcttcaaat ctcccccagt acggtgagcg tttacctaaa
     2581 gcgtgatcgt ccgtgatcga aacaagcctt atcagataat agcccccttt aggggggttc
     2641 ctgcgctctt gtcttttagc cttaaaaaat ccttttagcg cggatctttg gcgaggtgga
     2701 caactgcgcc tgtccgtcca tcgcccccct gttttttcgg ggggtgtggg cggcagcagt
     2761 tatccacgga gccgctcttt cccttgatct tgctctaggg cttgcagtat cccgcccatg
     2821 gttgaataat gtactaagca aggctacaca gaggcccctc tacctgcttc gcggtctcgg
     2881 gcctctgtgg ccttgttcag gggtgctacg cccccccgaa accccctgtg gcccccgctc
     2941 gatccgcccc ttcgggacgg tctgcgcggt ggcttcttgg ctctggagaa tggacgaggc
     3001 cctatgtcaa caacacagat agagaaagat tcgagaagga aggcagtcaa tggcgttatt
     3061 tctagtttcc aactagaggg ggaggccata accgatgaat tcaaaattct acttgaagat
     3121 tatatcaatg gaaaaatttc caccgatgag gtgttagcta tggtaagtga cgaacccaca
     3181 acaacggaat acgactacga aatcaccccc cgcactctcg gtcttggcgg gggctggaat
     3241 ctgcgtttgc tggagaacgg cgaagaggtc ggcgggggtg ttttcccctt gccggagcac
     3301 tgcgattttc gggatgaaaa ggcgctccag acgctactcg atagcctcta tgaggacgcc
     3361 ttagcggagg cctctgcatg gctggccagc cgttaaagcg cctccgctct atcaagatcc
     3421 gggtctcaga tgcggagctt gagcgcctgc gggagctctg ccccaaggcc caactggcgg
     3481 agtggatgcg ggagcaatgt ctcggtgttg cgcaacccca gcgccgaacg ccggcgccta
     3541 ccgtagatcc ggcgctcttg cggcagttgg ccggaatggg caacaacctg aaccagatcg
     3601 cccggcgcgt taattccggg gagtgggggc cgttggatcg gctgcgcatc atagcggagc
     3661 tgtccgctat aggccgcgag ctggaggaac tgcaccatga tcatcaaatt ccataaccgt
     3721 ggggtaggcc ggggctccgg gcctgttggc tacttgttgg gccgtgacgg gaaccgggaa
     3781 ggcgcaacgc tcgaccgggg caaccctgcg gtgattgaag ccctgattga ttccagtccc
     3841 tacgccaaaa aatacacctc tggcgttctg tcgtttgccg aggctgattt acccagggcg
     3901 accaaggaca agctgatgag cgactttgaa aaggcgctcc tgcccggact ggagggcaac
     3961 cagtacgctg ttttgtgggt agagcaccgc gacaaggggc ggctggagct gaattttgta
     4021 gtcccgaaca tcgaattgca gagcgggaag cggctgcaac cctacttcca caaggccgac
     4081 aatccgcgcc tcgatgcgtg gcgaaccgtg gtaaacgggg agctgaaatt gcacgacccg
     4141 gacgatccgt taaaccgtca ggccgttatc acgccaaagg atttgccgag ggggcacaag
     4201 gaggccgctc aggccattac agacgggctt ttacgcatgg ccggagcagg ggagatccag
     4261 agccgcgatg atgtgcgcaa agccttgcag ggtgcgggtt tcagcgtggt acgcgaaacc
     4321 aaaaacagcc tgagcattgc cgacccggac ggggggcgga acatccgatt aaagggggtg
     4381 ctgtatgagc aagattttag acatggcgaa ggacttcgag cagagctcga agcagcaggc
     4441 caacgctatc gagaagcgtc tgaccgaaga attcacgagg cacgagaaag ctatctccgg
     4501 ggcgttgaaa tcaagcgagg agaaaatgag cgccgctatt gccgagcaca gccagaaaat
     4561 gggctggctg ttgctcaaaa cgtggatctg gatgctggtc agcatcgtcc tgttcttgag
     4621 cgcaacgagc gggatcctct ggtatcaggg ggaattgata gcggagcggc aagccactct
     4681 ccaagccttg gcagagaagg gcggcaagct aaagctgacc cagtgcggga agaaattgga
     4741 gcacctttgc gtgcagatct ccgaccccaa tccaaccgtt tgggggaagg atacctattg
     4801 gatcgtgccg aaggggtact aaatgacggg attggagcgg gaattgctct gcgcgttcga
     4861 ggctttgcag agcgcatacg agcagcagca caaggcatgg caggaaagct acgcgaactt
     4921 ggcgagcatg tacgagagca ccaaacaaga ggccgagatc agcagggcag cagcagagcg
     4981 attgacgagg caggtgaacg gcttaacgca gcaagtggac ggcttgagca agcagctgga
     5041 gggcttgacc gggctagtca gcaagttgca aaagaaaggg aacacaggct cctgaaggca
     5101 ctggatcgag gctgggagcg tggagacaga tcgcgaggct ttgagcgttg acggaacaaa
     5161 acggccgtca gttataaaaa aaggaaagtg ctatcacttt ccttttatcg gcgggaatta
     5221 tcgttgagtt cttctatgcc atttttcata gtagtaactg taatcttcat ctctaccacc
     5281 agattcaatc cagtcatcat actcagggct atttttgaaa tctgaatcag acatgctaac
     5341 tccttgttat tagttggctt ctatggaagc tagcgcaccg tgaagttatg atcaacgatg
     5401 tagtccacat cattgtcgaa atgtgcgttc agctagtctt cggtgttttc tgctttattt
     5461 ctagcggcga ttctcgcgtc tgcccatgcg gcagactttt tagcctgttc tcgttgttct
     5521 tcggttgaag actcgctgtt gagaactttg ttgctcgttt gtttgaccac cgaatccaaa
     5581 cctgttgttg ctgcaaaatg agctaggtta acaactccct tacctaacaa cttggcacta
     5641 gcgattgcgg tttctttagc cccaaaagca tcagacacct cctcaagcgc tttagatagg
     5701 ttaaaaaatc ccatactccc cctaagaata tcatcaataa aattgagtgt tgctctgtgg
     5761 ataacttgca gagtttatta agtatcattg cagcaaagat gaaatcaatg atttatcaaa
     5821 aatgattgaa aggtggttgt aaataatgtt acaatgtgtg agaagcagtc taaattcttc
     5881 gtgaaatagt gatttttgaa gctaataaaa aacacacgtg gaatttaggt aatagttaaa
     5941 gcttgtgcct gattgttgcc acaattggtc agcagtttgc tagggaatga gagggcagag
     6001 aatgataagc ttagcactta atttgtatgg atatattgca ttaagcaagg ggacgttatg
     6061 cgtgtattag ccttatcggc tgtgtttttg gtggcatcga ttatcggaat gccagcggta
     6121 gcaaaggaat ggcaagaaaa c
//
