LOCUS       CP031375                8379 bp    DNA     circular BCT 22-MAY-2019
DEFINITION  Klebsiella pneumoniae strain KpvST101_OXA-48 plasmid pKpvST101_7,
            complete sequence.
ACCESSION   CP031375
VERSION     CP031375.2
DBLINK      BioProject: PRJNA483246
            BioSample: SAMN09729079
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Virulence genes in types of Klebsiella pneumoniae associated with
            antibiotic resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2018) Healthcare Associated Infection &
            Antimicrobial Resistance Division, Public Health England, 61
            Colindale Avenue, London, London NW9 5EQ, United Kingdom
REFERENCE   3  (bases 1 to 8379)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2019) Healthcare Associated Infection &
            Antimicrobial Resistance Division, Public Health England, 61
            Colindale Avenue, London, London NW9 5EQ, United Kingdom
  REMARK    Sequence update by submitter
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-JUL-2018
            Assembly Method :: miniasm v. 0.2-r168-dirty; racon correction (3
            iterations) v. 1.3.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 293.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/21/2019 15:23:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,176
            CDSs (total)                      :: 6,055
            Genes (coding)                    :: 5,577
            CDSs (with protein)               :: 5,577
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 478
            CDSs (without protein)            :: 478
            Pseudo Genes (ambiguous residues) :: 0 of 478
            Pseudo Genes (frameshifted)       :: 334 of 478
            Pseudo Genes (incomplete)         :: 141 of 478
            Pseudo Genes (internal stop)      :: 62 of 478
            Pseudo Genes (multiple problems)  :: 51 of 478
            ##Genome-Annotation-Data-END##
            On May 22, 2019 this sequence version replaced CP031375.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KpvST101_OXA-48"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKpvST101_7"
                     /geo_loc_name="United Kingdom"
                     /lat_lon="51.64 N 0.40 W"
                     /collection_date="02-Jun-2018"
                     /collected_by="Hospital in South East England"
                     /note="genotype: ST101"
     gene            complement(177..389)
                     /locus_tag="DWR33_30805"
     CDS             complement(177..389)
                     /locus_tag="DWR33_30805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QCS90027.1"
                     /translation="MAVKRCRRSRKTGFQHVQESGSSTAGNHVMASRRTTHHTIHRRLF
                     TRVVPRVAASKKQPNGESCPESGRY"
                     /name="hypothetical protein"
     gene            complement(707..1144)
                     /locus_tag="DWR33_30810"
     CDS             complement(707..1144)
                     /locus_tag="DWR33_30810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QCS90028.1"
                     /translation="MTTKNEEFQERLKASLKKANETLILKEKIESDISSILSMLKGITS
                     DSIDFDSFQNSSKIPKYKDCKKIVRIKKNSSMAYPKGFILFGFSINESTGYPVQVETED
                     EAAECTDVDNLKESIVYIIEKRSIEIMKLISEQQEDDDIPF"
                     /name="hypothetical protein"
     gene            complement(1128..2021)
                     /locus_tag="DWR33_30815"
     CDS             complement(1128..2021)
                     /locus_tag="DWR33_30815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="QCS90029.1"
                     /translation="MELNLCWWNIGISPPVKGVKKDKAEAIRLAKHYIKKMSQEKTLDF
                     LGICEISEDEAIDFKPLADKLHLEYLNLSGTVGRIIMDVSIMYEASKLEYIDYKYIIKI
                     QPDDRTLRIAVRVVFKDAVTGNLITFFLSHWPSIRSADEKIREDAAESLRNNVNKILDD
                     HGIESQIICMGDYNTNPYSVAINEKLYATRDYHRIKNKRKLLFNPFWYLLSDKKTNNLG
                     TYHYKSGASNRWFVFDQMLFSSSFIYGGKDCLKLDMNSLDFHRILSDDNQCLDQRFFNT
                     FDHYPIFCRIYHDNQK"
                     /name="endonuclease/exonuclease/phosphatase family protein"
     gene            complement(2317..2427)
                     /locus_tag="DWR33_30820"
                     /pseudo=""
     CDS             complement(2317..2427)
                     /locus_tag="DWR33_30820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(2459..2705)
                     /locus_tag="DWR33_30825"
                     /pseudo=""
     CDS             complement(2459..2705)
                     /locus_tag="DWR33_30825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579059.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            2849..3040
                     /locus_tag="DWR33_30830"
     CDS             2849..3040
                     /locus_tag="DWR33_30830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QCS90030.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3040..3429
                     /locus_tag="DWR33_30835"
     CDS             3040..3429
                     /locus_tag="DWR33_30835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QCS90031.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKF"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3721..3933)
                     /locus_tag="DWR33_30840"
     CDS             complement(3721..3933)
                     /locus_tag="DWR33_30840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QCS90032.1"
                     /translation="MAVKRCRRSRKTGFQHVQESGSSTAGNHVMASRRTTHHTIHRRLF
                     TRVVPRVAASKKQPNGESCPESGRY"
                     /name="hypothetical protein"
     gene            complement(4251..4460)
                     /locus_tag="DWR33_30845"
     CDS             complement(4251..4460)
                     /locus_tag="DWR33_30845"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QCS90033.1"
                     /translation="MAYPKGFILFGFSINESTGYPVQVETEDEAAECTDVDNLKESIVY
                     IIEKRSIEIMKLISEQQEDDDIPF"
                     /name="hypothetical protein"
     gene            complement(4672..5571)
                     /locus_tag="DWR33_30850"
                     /pseudo=""
     CDS             complement(4672..5571)
                     /locus_tag="DWR33_30850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /name="endonuclease/exonuclease/phosphatase family protein"
     gene            complement(5869..5982)
                     /locus_tag="DWR33_30855"
     CDS             complement(5869..5982)
                     /locus_tag="DWR33_30855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QCS90034.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPKRHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(6007..6397)
                     /locus_tag="DWR33_30860"
                     /pseudo=""
     CDS             complement(6007..6397)
                     /locus_tag="DWR33_30860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532398.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            6396..6587
                     /locus_tag="DWR33_30865"
     CDS             6396..6587
                     /locus_tag="DWR33_30865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QCS90035.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6587..6988
                     /locus_tag="DWR33_30870"
     CDS             6587..6988
                     /locus_tag="DWR33_30870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QCS90036.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7269..7481)
                     /locus_tag="DWR33_30875"
     CDS             complement(7269..7481)
                     /locus_tag="DWR33_30875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QCS90037.1"
                     /translation="MAVKRCRRSRKTGFQHVQESGSSTAGNHVMASRRTTHHTIHRRLF
                     TRVVPRVAASKKQPNGESCPESGRY"
                     /name="hypothetical protein"
     gene            complement(8111..8371)
                     /locus_tag="DWR33_30880"
     CDS             complement(8111..8371)
                     /locus_tag="DWR33_30880"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QCS90038.1"
                     /translation="MSQEKTLDFLGICEISEDEAIDFKPLADKLHLEYLNLSGTVGRII
                     MDVSIMYEASKLEYIDYKYIIKIQPDDRTLRIAVRVVFKEL"
                     /name="hypothetical protein"
     Typing          complement(2318..2728)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.029"
                     /coverage="99.0"
                     /evalue="1.6700000000000002e-161"
     Typing          complement(5870..6275)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.643"
                     /coverage="99.0"
                     /evalue="4.5799999999999997e-172"
ORIGIN
        1 accttcccaa aaatcgccag atctctgaac gctcctcacg actaaaaata cccacacact
       61 acgcaaaatt aaaattttgc agatttgtat ttaatatcag ttgtttactt tcctgaaatt
      121 tcgtttttta aattttattt acatagatca acgactcagg actataaaac aatgagttaa
      181 taacgacccg actctggaca cgactccccg tttggttgtt ttttagacgc ggcgactctg
      241 ggtacgactc tagtgaagag tcgccggtga atcgtgtggt gagtcgtccg gcgacttgcc
      301 ataacgtgat tgccggccgt cgacgaaccc gattcctgaa cgtgctgaaa accggttttc
      361 cggctgcgcc ggcaacgctt cacggccacg caataaattg cgtccccgtt caccgttttc
      421 ggatgggaag aacgccggaa acaggactta ccctggctga atggcgtgaa ttttcgcgct
      481 atgcacttgc gtgcatactc atgcatgccg taaaaacaga gcctgcgcgt ttctggcggg
      541 ttttcgggtg gtttgttgcc tgttttaccg gtttcccgtc agaaacgccc tgaggccgtt
      601 ttagcggtgc gtacaattat ggtcttatgt taaattagtt gattctaaga tatatggcac
      661 cttctatgaa aaagtgccgt tgataatggc attacatgct acctttttaa aaaggaatgt
      721 catcgtcttc ttgttgctcg gatattaatt tcattatctc tatagatctt ttttctatta
      781 tgtaaacaat tgattctttt aagttatcca catctgtaca ctctgcggct tcgtcttccg
      841 tttctacctg tacaggatat ccggtagact catttatgct aaatccaaat aaaatgaaac
      901 ctttagggta tgccatgcta gagtttttct ttattctgac aattttttta caatctttat
      961 atttgggaat tttcgaacta ttctgaaaac tatcaaagtc aattgagtcg gatgttatac
     1021 cctttagcat tgacaaaatt gatgaaatat ctgactctat tttttctttt aaaattagtg
     1081 tttcattggc tttttttagt gatgccttta gtctttcttg gaactcttca tttttggttg
     1141 tcatggtaaa tcctacaaaa tattgggtaa tgatcaaaag tgttaaaaaa tctttgatct
     1201 aaacattggt tatcatcact aagtatacga tgaaaatcaa ggctgttcat gtctagctta
     1261 agacaatctt ttcctccgta tataaatgat gatgaaaaca acatttgatc aaaaacaaac
     1321 catctattcg atgctcctga tttataatga taagttccta agttattggt ctttttgtct
     1381 gaaagtaaat accaaaaagg gttaaaaaga agttttcgtt tattctttat tcgatggtaa
     1441 tctctagtgg cgtaaagttt ttcgtttatt gcaacagagt aagggtttgt gttgtaatca
     1501 cccatgcata ttatttggga ctcaatgccg tggtcatcta gtattttatt cacattgttg
     1561 cgcagtgact cggcagcgtc ctctcgaatc ttttcatctg cgcttcttat ggatggccag
     1621 tgtgataaaa agaatgttat tagatttcct gttactgcat ccttaaaaac aactctgaca
     1681 gcaatacgca aagttctatc atctggttgt attttaatta tatatttata atctatgtac
     1741 tcaagttttg atgcttcgta cattatggat acatccataa ttatccgacc aacagtccct
     1801 gataaattca agtattcaag atgtagttta tcagccaaag gtttaaaatc tatagcctca
     1861 tcttctgata tttcacagat acccaaaaaa tccaatgttt tttcttgaga catttttttt
     1921 atatagtgct tggccaatct aatagcctca gccttgtctt tcttcacgcc ttttacaggt
     1981 ggtgatattc caatattcca ccaacataaa ttcaattcca tggtgtttct ttcttaatgc
     2041 attgtattca ttgggtttat tgtatgcttt tgatgtgggg ttaaacattt tatttatctg
     2101 tttgttgata aatggtgttg cgctacccct tttaaggcgt tatcttcttg gctggaaatg
     2161 aaaaaaccgc cttgcagggc ggttttttca agggttcaaa taacttgcag gtcttttgaa
     2221 cctaggtaac tggctagtag aggagcgcag tcaccaaaac ttgttctttc agtgtagcct
     2281 taactgaccc ataacttcaa gactaactcc tctaaatcag ttaccagtgg ctgctgccag
     2341 tggcgctttg tcgtgtcttt tcgggttgga ctcaagatga tagttaccgg ataaggcgca
     2401 gcagtcggac tgaacggggg gggggttcgt gcatacagtc cagcttggag cgaactgcct
     2461 acccggaact gagtgtcagg cgtggaatga gaaaaacgcg gccataacag cggaatgaca
     2521 ccggtaaacc gaatggcagg aacaggagag cgcacgaggg agccatcagg gggaaacgcc
     2581 tggtatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtcc gcttcggtga
     2641 tgcttgtcgg gggggcggag cctatggaaa aaacggccgc tttgcggcct ttccttcttc
     2701 cccatttgac cgctcggtct tctagagctt ttatacgcat gctcctgccg ctcgccgcag
     2761 ccgaacgacc ggagcgtagc gactgagtga gcgaggaagc ggaaaagaga ctgttcttac
     2821 actgagcact gacgctctga ggcttcttat gacgaaacaa cagaaaacag cgctgaacat
     2881 ggctaaattc atccagaatc agtctctgct cctgctggag aagcttaacg agcttgatct
     2941 tgatgtcgag gccgaccttt gtgagaaact ccacgatgat gcggagcatc ttttccgtac
     3001 actgtcctcg agactggatg cattacagga tggtaactga tggccagagt gtctattagt
     3061 gaggctgccc gactgacggg taagagtaga acaacactgc acaggttaat taaagcaggt
     3121 gacctgtcca cctgtagcgg tgaacgaaat gcaaaaatgg tagatacttc agagctttta
     3181 cgtgtctttg gtccctttga acaaccgaag ggtgaacacc attctggaca ggtctctgaa
     3241 cagcgtgata cagcctctgc tgaccagagt gaacaggtga ttcgtcatct gaaacaggag
     3301 attgaacacc tgaatacctt acttttgtcc aaggattcgc acattgacag cctgaaacag
     3361 gccatgctcc ttcttgaaaa tcaccaggca aaattaacgg aatccccggc accatggtgg
     3421 aaattctgaa aaaaatcctg atcctgccat gcttgagttt tcctctccgg ccatcccggg
     3481 gagttcctga accttcccaa aaatcgccag atctctgaac gctcctcacg actaaaaata
     3541 cccacacact acgcaaaatt aaaattttgc agatttgtat ttaatatcag ttgtttactt
     3601 tcctgaaatt tcgtttttta aattttattt acatagatcc gactctatga cgactcgtca
     3661 tagagtcccc ggcgagtcgt catagagtcg gtaacgactc aggactataa aaacaatgag
     3721 ttaataacga cccgactctg gacacgactc cccgtttggt tgttttttag acgcggcgac
     3781 tctgggtacg actctagtga agagtcgccg gtgaatcgtg tggtgagtcg tccggcgact
     3841 tgccataacg tgattgccgg ccgtcgacga acccgattcc tgaacgtgct gaaaaccggt
     3901 tttccggctg cgccggcaac gcttcacggc cacgcaataa attgcgtccc cgttcaccgt
     3961 tttcggatgg gaagaacgcc ggaaacagga cttaccctgg ctgaatggcg tgaattttcg
     4021 cgctatgcac ttgcgtgcat actcatgcat gccgtaaaaa cagagcctgc gcgtttctgg
     4081 cgggttttcg ggtggtttgt tgcctgtttt accggtttcc cgtcagaaac gccctgaggc
     4141 cgttttagcg gtgcgtacaa ttatggtctt atgttaaatt agttgattct aagatatatg
     4201 gcaccttcta tgaaaaagtg ccgttgataa tggcattaca tgctaccttt ttaaaaagga
     4261 atgtcatcgt cttcttgttg ctcggatatt aatttcatta tctctataga tcttttttct
     4321 attatgtaaa caattgattc ttttaagtta tccacatctg tacactctgc ggcttcgtct
     4381 tccgtttcta cctgtacagg atatccggta gactcattta tgctaaatcc aaataaaatg
     4441 aaacctttag ggtatgccat gctagagttt ttctttattc tgacaatttt tttacaatct
     4501 ttatatttgg gaattttcga actattctga aaaatatcaa agtcaattga gtcggatgtt
     4561 atacccttta gcattgacaa aattgatgaa atatctgact ctatttattc ttttaaaatt
     4621 agtgtttcat tggctttttt tagtgatgcc tttagtcttt cttggaactc ttcatttttg
     4681 gttgtcatgg taaatcctac aaaatattgg gtaatgatca aaagtgttaa aaaatctttg
     4741 atctaaacat tggttatcat cactaagtat acgatgaaaa tcaaggctgt tcatgtctag
     4801 cttaagacaa tcttttcctc cgtatataaa tgatgatgaa aacaacattt tatcaaaaac
     4861 aaaccatcta ttcgatgctc ctgatttata atgataagtt cctaagttat tggtcttttt
     4921 tttttgtctg aaagtaaata aaaaaaaggg ttaaaaaaga agttttcgtt tattctttat
     4981 tcgatggtaa tctctagtgg cgtaaagttt ttcgtttatt gcaacagagt aagggtttgt
     5041 gttgtaatca cccatgcata ttatttggga ctcaatgccg tggtcatcta gtattttatt
     5101 cacattgttg cgcagtgact cggcagcgtc ctctcgaatc ttttcatctg cgcttcttat
     5161 ggatggccag tgtgataaaa agaatgttat tagatttcct gttactgcat acttaaaaac
     5221 aactctgaca gcaatacgca aagttctatc atctggttgt attttaatta tatatttata
     5281 atctatgtac tcaagttttg atgcttcgta cattatggat acatccataa ttatccgacc
     5341 aacagtccct gataaattca agtattcaag atgtagttta tcagccaaag gtttaaaatc
     5401 tatagcctca tcttctgata tttcacagat acccaaagaa tccaatgttt tttcttgaga
     5461 catttttttt atatagtgct tggccaatct aatagcctca gccttgtctt tcttcacgcc
     5521 ttttacaggt ggtgatattc caatattcca ccaacataaa ttcaattcca tggtgtttct
     5581 ttcttaatgc attgtattca ttgggtttat tgtattgctt ttgatgtggg ggttaaacat
     5641 tttatttatc tgtttgttga taaatggtgt tgcgctaccc cttttaaggc gttatcttct
     5701 tggctggaaa tgaaaaaacc gccttgcagg gcggtttttt caagggttca aataacttgc
     5761 aggtcttttg aacctaggta actggctagt agaggagcgc agtcaccaaa acttgttctt
     5821 tcagtgtagc cttaactgac ccataacttc aagactaact cctctaaatc agttaccagt
     5881 ggctgctgcc agtggcgctt tgtcgtgtct tttcgggttg gactcaagat gatagttacc
     5941 ggataaggcg cagcagtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg
     6001 aactgcctac ccggaactga gtgtcaggcg tggaatgaga aaaacgcggc cataacagcg
     6061 gaatgacacc ggtaaaccga atggcaggaa caggagagcg cacgagggag ccatcagggg
     6121 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtccgc
     6181 ttcggtgatg cttgtcaggg gggcggagcc tatggaaaaa cggccgcttt gcggcctttc
     6241 cttcttcccc atttgaccgc tcggtcttct cgagctttta tacgcatgct cctgccgctc
     6301 gccgcagccg aacgaccgga gcgtagcgac tgagtgagcg aggaagcgga aaagagactg
     6361 ttcttacact gagcactgac gctctgaggc ttcttatgac gaaacaacag aaaacagcgc
     6421 tgaacatggc taaattcatc cagaatcagt ctctgctcct gctggagaag cttaacgagc
     6481 ttgatcttga tgtcgaggcc gacctttgtg agaaactcca cgatgatgcg gagcatcttt
     6541 tccgtacact gtcctcgaga ctggatgcat tacaggatgg taactgatgg ccagagtgtc
     6601 tattagtgag gctgcccgac tgacgggtaa gagtagaaca acactgcaca ggttaattaa
     6661 agcaggtgac ctgtccacct gtagcggtga acgaaatgca aaaatggtag atacttcaga
     6721 gcttttacgt gtctttggtc cctttgaaca accgaagggt gaacaccatt ctggacaggt
     6781 ctctgaacag cgtgatacag cctctgctga ccagagtgaa caggtgattc gtcatctgaa
     6841 acaggagatt gaacacctga ataccttact tttgtccaag gattcgcaca ttgacagcct
     6901 gaaacaggcc atgctccttc ttgaaaatca ccaggcaaaa ttaacggaat ccccggcacc
     6961 atggtggaaa ttctggaaaa aatcctgatc ctgccatgct tgagttttcc tctccggcca
     7021 tcccggggag ttcctgaacc ttcccaaaaa tcgccagatc tctgaacgct cctcacgact
     7081 aaaaataccc acacactacg caaaattaaa attttgcaga tttgtattta atatcagttg
     7141 tttactttcc tgaaatttcg ttttttaaat tttatttaca tagatccgac tctatgacga
     7201 ctcgtcatag agtccccggc gagtcgtcat agagtcggta acgactcagg actataaaaa
     7261 acaatgagtt aataacgacc cgactctgga cacgactccc cgtttggttg ttttttagac
     7321 gcggcgactc tgggtacgac tctagtgaag agtcgccggt gaatcgtgtg gtgagtcgtc
     7381 cggcgacttg ccataacgtg attgccggcc gtcgacgaac ccgattcctg aacgtgctga
     7441 aaaccggttt tccggctgcg ccggcaacgc ttcacggcca cgcaataaat tgcgtccccg
     7501 ttcaccgttt tcggatggga agaacgccgg aaacaggact taccctggct gaatggcgtg
     7561 aattttcgcg ctatgcactt gcgtgcatac tcatgcatgc cgtaaaaaaa cagcgcctgc
     7621 gcgtttctgg cgggttttcg ggtggtttgt tgcctgtttt accggtttcc cgtcagaaac
     7681 gccctgaggc cgttttagcg gtgcgtacaa ttatattctg acaatttttt tacaatcttt
     7741 atatttggga attttcgaac tattctgaaa aatatcaaag tcaattgagt cggatgttat
     7801 accctttagc attgacaaaa ttgatgaaat atctgactct attttttctt ttaaaattag
     7861 tgtttcattg gcttttttag tgatttttta gtctttcttg aactcttttt tggttgtcat
     7921 ggaactacaa aatattgggt aatgattttt aaaaaaatct ttgatctaaa cattggttat
     7981 catcactaag tatacgatga aaatcaaggc tgttcatgtc tagcttaaga caatcttttc
     8041 ctccgtatat aaatgatgat gaaaacaaca tttgatcaaa aaaacaaacc atctattcga
     8101 tgctcctgat ttataattcc ttaaaaacaa ctctgacagc aatacgcaaa gttctatcat
     8161 ctggttgtat tttaattata tatttataat ctatgtactc aagttttgat gcttcgtaca
     8221 ttatggatac atccataatt atccgaccaa cagtccctga taaattcaag tattcaagat
     8281 gtagtttatc agccaaaggt ttaaaatcta tagcctcatc ttctgatatt tcacagatac
     8341 ccaaaaaatc caatgttttt tcttgagaca ttttttttt
//
