LOCUS       CP030577                3126 bp    DNA     circular BCT 10-OCT-2019
DEFINITION  Staphylococcus aureus strain ER01881.3 plasmid unnamed1, complete
            sequence.
ACCESSION   CP030577
VERSION     CP030577.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN09483966
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Sullivan,M.J., Altman,D.R., Chacko,K., Cliferri,B., Webster,E.,
            Deikus,G., Lewis,M., Khan,Z., Beckford,C., Rendo,A., Samaroo,F.,
            Sebra,R., Karam-Howlin,R., Southwick,K., Adams,E., Ying,L.,
            Kornblum,J., Factor,S., Danesh Yazdi,M., Dingle,T., Hamula,C.,
            Bashir,A., Schadt,E., Kasarskis,A., Patel,G., Wallach,F., Gibbs,K.
            and van Bakel,H.
  TITLE     Whole genome sequencing to identify and define MRSA outbreaks
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Sullivan,M.J., Altman,D.R., Chacko,K., Cliferri,B., Webster,E.,
            Deikus,G., Lewis,M., Khan,Z., Beckford,C., Rendo,A., Samaroo,F.,
            Sebra,R., Karam-Howlin,R., Southwick,K., Adams,E., Ying,L.,
            Kornblum,J., Factor,S., Danesh Yazdi,M., Dingle,T., Hamula,C.,
            Bashir,A., Schadt,E., Kasarskis,A., Patel,G., Wallach,F., Gibbs,K.
            and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2018) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 227.433493301x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2018 17:29:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,992
            CDS (total)                       :: 2,910
            Genes (coding)                    :: 2,828
            CDS (coding)                      :: 2,828
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 43 of 82
            Pseudo Genes (incomplete)         :: 35 of 82
            Pseudo Genes (internal stop)      :: 23 of 82
            Pseudo Genes (multiple problems)  :: 18 of 82
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ER01881.3"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2015"
                     /collected_by="Mount Sinai Hospital"
     gene            join(2940..3126,1..2)
                     /locus_tag="DQU85_00005"
     CDS             join(2940..3126,1..2)
                     /locus_tag="DQU85_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QFL76736.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(328..909)
                     /locus_tag="DQU85_00010"
     CDS             complement(328..909)
                     /locus_tag="DQU85_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QFL76733.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNLYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     gene            1361..2356
                     /locus_tag="DQU85_00015"
     CDS             1361..2356
                     /locus_tag="DQU85_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QFL76734.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            complement(2363..2554)
                     /locus_tag="DQU85_00020"
     CDS             complement(2363..2554)
                     /locus_tag="DQU85_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001791934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QFL76735.1"
                     /translation="MIGLAKPEIPGVLRYFKVISYKAVLSYFCDTIYCVCLIFVTLHTA
                     FVTKIRQYFFIKNQQKRH"
                     /name="hypothetical protein"
     Typing          2427..2591
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          1362..2356
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agaatatttg aggggatttt gaaacgagtt tcttcttgtt ttcacactgt ttttttattc
       61 ctattggtgt tgttgcttac tttttgtttt tctataaaga tgatgctttt gacgagatgg
      121 aagaaaaata tgattatcat gaagataata aaaaatagac gacgcattta tgccgagaaa
      181 atttattgat gttgagaaga acccttaact aaacttgcag acgaatgtcg gcatagcgtg
      241 agctattaag ccgaccattc gacaagtttt gggattgtta agggttccga ggctcaacgt
      301 caataaagca attggaataa atagtatcta aagtaacccg tatttaagat tacattgtac
      361 atccttcgaa accatagtag ctgcgattcc agctaggttt ccttttatcc caattttagc
      421 taattttttt gctacgccct ttaagttttt ggctttaaca tcttcaaaaa ctgctgtgat
      481 tatgtttcca gttaaaagtt ctccatataa gtttaaaact tcagaataga aacagtcacc
      541 gctatttttg tagttcccac caactgctct aggtgatatt gaactagttt tctttttatt
      601 ctctatgtcg ttttttagtt tttggaatcc ttcaggaaca tatccatatc gagatttggc
      661 attttctaga ttaacatctt tagctgttcc gtctttatct aaaataataa ttttgccata
      721 tatgtatttt agttcttttt caatttcttt ttcgtttaaa gtttcaacgt ttttatctgc
      781 aatgttattt ttgttttggg cttgagcttc tttgaaatta aaatccaaga ccattgaaag
      841 tatggttaaa atacttaaag atactactaa aatctttctt agtgatatac tgttttgaat
      901 tgcattcatt gtaaatgccc ccttgttttg ttatttaaat gaattcaata taatattaac
      961 atataaaaac aaaggagttt aatatgaata agttttttta ctttttgatt attttattat
     1021 ctgtattttt aatttttaat tttgttaatt cgattttatt tgataatcct tttgactttt
     1081 tagaaagttt tatttattca ttggttttca cattaattta tttaggttta gagggtcata
     1141 aaaagaagaa ggaataggtt gttttttgaa acgagtgtga acgagtttct tcttgtcttg
     1201 atactatata gaaataactc gattttatat atatagctgt aactgttgat attacagtgt
     1261 ttaaacgtgt ttttgtgcgt gaaaggaaaa tttgacaata aaaaacccca gttatattat
     1321 taaggtgtcg aatcttaaat aatactgggg gtctttttat atgcaatata atactactag
     1381 aagtataacc gaaaatcaag ataataaaac gttaaaagat atgacgaaaa gtgggaaaca
     1441 acgcccatgg agagaaaaga aaatagataa tgtaagctat gcagatatac tagaaatttt
     1501 aaaaatcaaa aaggctttta atgtaaaaca atgtggtaat attttagaat ttaagccaac
     1561 tgatgaaggc tatttgaagt tacataagac atggttttgt aaatcaaaat tatgtccggt
     1621 ttgtaattgg agacgtgcta tgaaaaatag ttatcaagct caaaaagtga ttgaaaaagt
     1681 aattaaggaa aagccaaaag cacgttggtt gtttttaaca ctttcaacaa aaaatgcgat
     1741 agatggagat actttagaac aaagtttgaa gcatctaact aaagcatttg ataggttgag
     1801 tagatataaa aaggttaaac aaaatcttgt tggatttatg cgttcaacag aagttaccgt
     1861 taataaaaat gacggtagtt ataatcagca catgcatgtt ttgttatgtg ttgaaaatgc
     1921 atattttaga aaaaaagaga attatataac tcaagaagaa tgggttaatt tatggcaaag
     1981 agcattacaa gttgactatc gacctgttgc taatgttaaa gcgatcaaac cgaatagaaa
     2041 aggcgataaa gacattgaat cggcaatcaa agagacctca aaatattcgg ttaaatcatc
     2101 tgatttttta actgatgatg atgaaaaaaa tcaagaaatt gtaagtgatt tagaaaaagg
     2161 tttgtatcga aaacgtatgt taagttatgg tggattgctt aaacaaaaac ataaaatttt
     2221 aaacttagac gatgtcgaag atggtaattt gattaatgca agtgatgaag ataaaacaac
     2281 agacgaagaa gaaaaagcac attcaattac cgcaatttgg aatttcgaaa agcaaaatta
     2341 ttatttaaga cattagtgtt gactaatgtc ttttttgttg attttttata aaaaagtact
     2401 gtcttatttt tgtgacaaat gctgtatgta gtgtcacaaa aataagacaa acgcaatata
     2461 ttgtgtcaca aaaataagac agtacagctt tgtatgatat cactttaaaa taccttaaaa
     2521 cccctggaat ttctggcttt gccagaccta tcatttttga atgatagcaa attctcctta
     2581 tgctcttacg gagtttttag agaaaaatta aaaattctcg atttttgata aaaaacgccc
     2641 tgcaggaatt tagaaaaaca tgtggaagtt ttaaaggatt ttatgctaat ttttaatttg
     2701 catgtaaatg ggcagtgtta aaaaattagt cactggtttt gctgaaattt ttgtttgtga
     2761 aattaccata tatttatttg gctcatattt gcgttttaag agcttgtata tatttttaag
     2821 ttaaaaacct atatgatacc ccctaaacct ttaaaatacc cccttaaaac tcaaaataaa
     2881 ggtatttata atttgagtaa gtcttgtgat aaagtttttt taaaaaagga gtgtttattt
     2941 tgaataaaaa cacagtgtta gatgaaggtt acgttatagc aactatttta aatgttttct
     3001 ttttacttgg attaattttt atatctcatt tggaaaattt atatatatta attccgtatg
     3061 ttattttgat gggaataaat gctatttatt tggttgttaa ggttatgaat ttcaaaaaga
     3121 ataatt
//
