LOCUS       CP030576               13163 bp    DNA     circular BCT 10-OCT-2019
DEFINITION  Staphylococcus aureus strain ER03910.3 plasmid unnamed1, complete
            sequence.
ACCESSION   CP030576
VERSION     CP030576.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN09484029
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Sullivan,M.J., Altman,D.R., Chacko,K., Cliferri,B., Webster,E.,
            Deikus,G., Lewis,M., Khan,Z., Beckford,C., Rendo,A., Samaroo,F.,
            Sebra,R., Karam-Howlin,R., Southwick,K., Adams,E., Ying,L.,
            Kornblum,J., Factor,S., Danesh Yazdi,M., Dingle,T., Hamula,C.,
            Bashir,A., Schadt,E., Kasarskis,A., Patel,G., Wallach,F., Gibbs,K.
            and van Bakel,H.
  TITLE     Whole genome sequencing to identify and define MRSA outbreaks
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13163)
  AUTHORS   Sullivan,M.J., Altman,D.R., Chacko,K., Cliferri,B., Webster,E.,
            Deikus,G., Lewis,M., Khan,Z., Beckford,C., Rendo,A., Samaroo,F.,
            Sebra,R., Karam-Howlin,R., Southwick,K., Adams,E., Ying,L.,
            Kornblum,J., Factor,S., Danesh Yazdi,M., Dingle,T., Hamula,C.,
            Bashir,A., Schadt,E., Kasarskis,A., Patel,G., Wallach,F., Gibbs,K.
            and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2018) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400.147836197x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2018 17:31:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,959
            CDS (total)                       :: 2,877
            Genes (coding)                    :: 2,780
            CDS (coding)                      :: 2,780
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 35 of 97
            Pseudo Genes (internal stop)      :: 28 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ER03910.3"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2015"
                     /collected_by="Mount Sinai Hospital"
     gene            join(13163,1..983)
                     /locus_tag="DQV48_14725"
     CDS             join(13163,1..983)
                     /locus_tag="DQV48_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="QFL82328.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication protein"
     gene            976..1292
                     /locus_tag="DQV48_14730"
                     /pseudo=""
     CDS             976..1292
                     /locus_tag="DQV48_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /name="replication protein"
     gene            complement(2156..2848)
                     /locus_tag="DQV48_14735"
     CDS             complement(2156..2848)
                     /locus_tag="DQV48_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QFL82329.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="hypothetical protein"
     gene            2958..3152
                     /locus_tag="DQV48_14740"
     CDS             2958..3152
                     /locus_tag="DQV48_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QFL82330.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            4268..4885
                     /locus_tag="DQV48_14745"
     CDS             4268..4885
                     /locus_tag="DQV48_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium transporter"
                     /protein_id="QFL82331.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQYL
                     GSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNEKG
                     LSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQKLAN
                     IPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
                     /name="cadmium transporter"
     gene            4904..5251
                     /locus_tag="DQV48_14750"
     CDS             4904..5251
                     /locus_tag="DQV48_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="QFL82332.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="transcriptional regulator"
     gene            5408..5500
                     /locus_tag="DQV48_14755"
     CDS             5408..5500
                     /locus_tag="DQV48_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="QFL82333.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5672..6643)
                     /locus_tag="DQV48_14760"
     CDS             complement(5672..6643)
                     /locus_tag="DQV48_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="QFL82334.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="NADPH:quinone reductase"
     gene            6767..7186
                     /locus_tag="DQV48_14765"
     CDS             6767..7186
                     /locus_tag="DQV48_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="QFL82335.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            complement(7408..7503)
                     /locus_tag="DQV48_14770"
                     /pseudo=""
     CDS             complement(7408..7503)
                     /locus_tag="DQV48_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560862.1"
                     /note="incomplete; partial on complete genome; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /name="O-antigen ligase domain-containing protein"
     gene            complement(7712..8557)
                     /locus_tag="DQV48_14775"
     CDS             complement(7712..8557)
                     /locus_tag="DQV48_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="QFL82336.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALNT
                     KSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEKYV
                     GKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIELNYY
                     SPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPKDYKV
                     ADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKSVMKEF
                     "
                     /name="penicillin-hydrolyzing class A beta-lactamase BlaZ"
     gene            8664..10421
                     /locus_tag="DQV48_14780"
     CDS             8664..10421
                     /locus_tag="DQV48_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="QFL82337.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTMLA
                     GLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSIDN
                     ICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIVIRK
                     AEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFNIFSI
                     IMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSNKNILS
                     HSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSLTDYNYK
                     KPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYKIYLALFG
                     LDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIPKNYTAAQL
                     KQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQLSSSLLIKK
                     NEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGKNAELISEKIL
                     KGMGVLNDQ"
                     /name="beta-lactam sensor/signal transducer BlaR1"
     gene            10411..10791
                     /gene="blaI"
                     /locus_tag="DQV48_14785"
     ARG             10412..10788
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            11055..11633
                     /locus_tag="DQV48_14790"
     CDS             11055..11633
                     /locus_tag="DQV48_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="QFL82339.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="HTH domain-containing protein"
     gene            complement(11994..12737)
                     /locus_tag="DQV48_14795"
     CDS             complement(11994..12737)
                     /locus_tag="DQV48_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="QFL82340.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     ARG             8665..10418
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     ARG             complement(7716..8557)
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             4269..4882
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(7713..8557)
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          1349..1496
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.980000000000001e-72"
     Typing          2..983
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1115..1284
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.605"
                     /coverage="91.0"
                     /evalue="1.04e-65"
     Typing          complement(11995..12737)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgcaaaatca atattttaca gtgcaagaaa attacaaaga aagattttat caaatcccta
       61 aagtattctt taccagtgaa aattataaaa atctaactaa tgatatgaaa atagcctatg
      121 ctatcttacg tgatcgctta aatttatcta ttaaaaatag ctgggttgat gaagatggta
      181 acatttactt tgtttattca aatgaaaagc taatggaaat cttaaattgt aaaaaagaaa
      241 agttaacaaa aataaaaaaa gggttagaaa acgatggtct actcattcaa aaacgacgtg
      301 gtttaaataa accaaatatt ctatatttaa tgaaacctat tgtcacagaa cgtgatattt
      361 acaaaataga aaaagaagaa aacgatgttg aaccttacgg cgagaaggaa gttcgaaaat
      421 cgaacgtcca gaagttcgaa aatcgaacgt ccagaagttc gaaaatcgaa cgtccagaag
      481 ttcgaaaatc gaacactaat gatactgact ttaatgacat tgactttaat gatactgaga
      541 ataatgatat gaatgacttg aatgatataa agtacaaaaa tgaaattagt aatcattcaa
      601 atcattcaaa tcaatttaca cataattttg atgataaaga aatgctattg caagaattcc
      661 cagaacaact aactaattat ttattaaaat atgattatag agatttagaa atcataaagg
      721 ctgttattct taaagcaaaa aaatcattta attcaaggca tgaagatatg cattacatgt
      781 tagaggacat tgaggatgaa atattaactt ctttaaaaag acttaaaaaa gctatacatg
      841 ataggggagt taaaggccaa aaggaaacaa tcaagtcaat gcaagcttat ttaatgcaaa
      901 caattctaac tgaacttgaa gaaacacatg ccctttatat gcgccgtaaa aacatgaaac
      961 aatataatat ctttaatcaa taattcaaat gattttataa cactcgaaga gacatcaaaa
     1021 tattccgtta aatcatctga ctttttaact gatgacgacg aaaaaaatca agaaattgta
     1081 agtgatttag aaaaaggttt gtatcgaaaa cgtatgttaa gttatggtgg attgcttaaa
     1141 caaaagcata aaattttaaa cttagacgat gccgaagatg gtaatttgat taatacaagt
     1201 gatgaagata aaacgacaga cgaagaaaaa aagcacattc aattaccgca atttggaatt
     1261 tcgaaaagca aaattattat ttaagacatt agtgataact gatgtctttt tttattgttt
     1321 ttctgtaaaa aagtgctgtc ttatttttgt gacaaatgct gtatgtagtg tcacaaaagt
     1381 gtgacactac agctttgtat gatatcactt taaaataact taaaaccctt ggaatgtctg
     1441 gccttgccag atctatcatt tttgaatgat agcaaatttc ccttatgctc ttacggagat
     1501 tttagagtta aattaaaatt tctaagtttt tgataagaaa agccctgcag gaatttagaa
     1561 aaacatgggg aagttttaaa gtattttatg ctaattttta aatttgcatg taaatgggca
     1621 gtatctaaaa aattagtcac tgattttgct gaaatttttg tttgtgaaat tagcatatat
     1681 ttatttggct catatttgcg ttttaagaga ctgtatagat ttttaagtac aaaacctata
     1741 tgatttgcct ctaagtcttc aaaatgcctc ttaaaatcca aaataaaagt gtttaatatt
     1801 gtaataagga ttgctatttt tataaaagga gagatatcat gaagattaaa actttcgcta
     1861 cttttactca tcgtcaatta gataaacaag ttaataggtt tttaaagaat gatattcatg
     1921 tattagaaat tcaaacacac agtaattggt tatatatttc agctattgtt ttttatgagg
     1981 ataaataagg aacatcgtat ttatgccgaa aaaatttatt gatgttgaga agaaccctta
     2041 actaaacttg cagacgaatg tcggcatagc gtgagctatt aagccgacca ttcgacaagt
     2101 tttgggattg ttaagggttc cgaggctcaa cgtcaataaa acaattggaa taaaatcaac
     2161 ctacagagca tccagctgca aatgcagttc cgcaaactgc ttcacataca ccgccggcta
     2221 ttggatttac gattgcccat actccacagt agtgagcaca tgcataaagt ccagccatac
     2281 tacaagcgaa tgctttgccg ttccatttga atcctgcata tgttaccggt ctgtttttat
     2341 caactttttc agccttatat tcaacgtaat ttttgttaat aagtattttt ccctctttgt
     2401 tttcagtcat acttcttttt tcggcaatga attttgtgat ttttttgttg tagttatcat
     2461 atattgtttc agaaactaca atttcttttg ttttttcatt tttataagcg ttattagcta
     2521 tgtagccata tgcattttta tcatttgctt ctgtagctag atattttaat ttagaatttt
     2581 tgtcttctag ctttattttt ttaaatcctt cttttttcat ttgcttttcg aatttttgca
     2641 tatcttcatt tttattttgc aattgttcta gaaaacttat ttctttcttg gtcatttgtg
     2701 atacttcgat gtttttgctt ttcaattttt ctttttgatt ttctagtaaa gaactttttt
     2761 gttcttcatt tgcgtaagca ttagaaaaat tcgcgctaaa taccagtagg aacaaggaaa
     2821 ttattgaaaa taattttata attttcattt gatacctcca agataataag atattttaat
     2881 tgtataagta agcgctttat aaaatcaata tttattttaa tatatttata aacatatttt
     2941 tgaaaggagt ctattttatg aatgaaaatt tagaaaaata tataaaaagt ttgccacttt
     3001 taggtttgtt aattagtatt ttgttaattg ttttattctt ttttatttta gatgttgatg
     3061 aagatttatt cgtggttttt ttatactgtt tattgccttt gatagttaat ttgtctattt
     3121 atggttttta tgtttttgtt aaaagaaagt aagattatga ataattttgt ttttaatttg
     3181 tatgctttga gggggttgga aaacggtagg tgtatagatt agatttcttg tttttttgaa
     3241 acgagtgtga acgagtttct tcttgtcttg atttttgata aaaacgccct acaggaattt
     3301 agaaaaacgt ggggaagttt taaagaattt tatgctaatt tttaatttgc atgtaaatgg
     3361 gcagtgtcta aaaatagtta ttgtttttgc tcaaattttt gtttttgaaa atagcatata
     3421 tttatttggc tcatatttgc gttttaaaag catatataag ttttagatat aaaactatat
     3481 gaatcacccc taaatcttta aaatgccctc ttaaaattca aaataagggc ataaaaaata
     3541 aaagctaccc cagataaagt gaggtagctt tttctttcat acggtctatt tatttttctt
     3601 tcgacgccgt atcgtcgatg taattcaaaa aagttatggg ctataaatct acatcacttt
     3661 ttcaagaatg tagtagcaat attcaacgca aattaatttt tataactgct cggaatatct
     3721 caagccgttt ctttaatttt gaaataaaaa aatcgacgaa ggtcgattac gtttttgcac
     3781 atgtcgattt atccgacgta taagtgcgcc cttacgggat ttaactagat tataacgacg
     3841 aattttagac ctgtaaagca ataacaatta ggtctaccag gtagataaag tgtacaaagt
     3901 agaaccgaaa aagttattta ttttgaagat ttttcgtaga tataatttgc taataaactt
     3961 cctaaaactg aataaagaaa aatagttaaa ctcatgtaat cactccataa ttctgaagtg
     4021 tctccatgtc tacgcataga cacgcattta tgccgaaaaa atttattgat gttgagaaga
     4081 acccttaact aaacttgcag acgaatgtcg gcatagcgtg agctattaag ccgaccattc
     4141 gacaagtttt gggattgtta agggttccga ggctcaacgt caataaagca attggaataa
     4201 agcacctatg attatttaaa aatataagtt ataatacatt caaaaatatt tttgaatgag
     4261 gtgtattatg attcaaacgg ttgttgctgc tgctgttctt tatattgcta cagcagttga
     4321 tttattagta attttattaa tattttttgc tagagcaaag actagaaaag aatatagaga
     4381 tatttatgtt ggtcaatatt tagggtctat tattttaata ttagttagtt tgtttttagc
     4441 ttttgtatta aattatgttc cagaaaagtg gatattaggt ttattgggtt taataccaat
     4501 ttatcttgga attaaagtgg ctatttatga tgattgtgaa ggagaaaaga gagctaaaaa
     4561 agaattgaat gaaaaaggat tgtctaaatt agttggtacg gttgcaattg ttacgatagc
     4621 aagttgtggc gccgataata ttggtttatt tgttccgtat tttgtgacat taagtgttac
     4681 taatttatta cttactctgt ttgtcttttt aattttaatt ttcttcttgg tgtttactgc
     4741 acaaaaatta gctaatattc caggaattgg agaaattgtt gagaaattta gtcgttggat
     4801 tatggctatt atttatatag ctttaggttt atttattatt attgaaaatg acactattca
     4861 aacaatttta ggatttatat tttaatttag ggtgtgattt tatatgagtt atgaaaatac
     4921 ttgtgatgtg atctgtgtac atgaggataa agttaacaat gctttaagtt ttttagaaga
     4981 tgataaatct aagaaattac ttaacatttt agaaaaaatt tgtgatgaga agaaattgaa
     5041 aatcatatta tctttgatta aagaagatga gttgtgtgtt tgtgatattt ctttgatatt
     5101 gaaaatgagt gttgcttcaa cttcacatca tttaaggctt ttatataaaa atgaggtact
     5161 tgatttttat aaagatggaa agatggcata ttattttatt aaagacgatg aaataagaga
     5221 gtttttctct aaaaatcatg agggtttttg aaacgagttt cttcttgtct tgatactata
     5281 aaaaacaaag ttatataaca taagacgact tatcggaagt agatatgctt gtatattcac
     5341 agatggtata ataatagagt cgcctatctc tcaggcgtca attgacgaag agaggaggtg
     5401 cattactttg ctaatcattt tcgttcacat catagcacca gtcatcagtg gctgtgctgt
     5461 tgcgtattat acttattggc ttagtaaacg caacaaataa ttaataggcg actttataag
     5521 ccagcatcga aaaaatcccc acactactgc aatagtgtgg ggattggtgc attaattgtt
     5581 aatcattttc gttatataaa ttataacact ggttagtaga gaaatgcaaa atgattgaat
     5641 taatagtaaa tgtattaatg aatactcaca cttatacttt taaaatagtt cttcctaaat
     5701 gttgtccttt taaaagctga tctatcgttt tatagaattc ttcaaatcca atttcattgt
     5761 atagaagtga atttcctata ttccattcat ttgctaactt actccatata tcttcacgga
     5821 tattatgatc aacatataca gaatcaatgc caattaattt aatacctcta agaataaacg
     5881 gtaatatatt agcgttaagt gaatttcctg ctacatttcc acatacagta gcaacacctt
     5941 gataatttag acgttttaaa ccgtataatg ttacattact ccccactgta tctaagaaat
     6001 aatcaaaaat acctttttta agcggtttat tatcatcttc aattattatt acttgttttg
     6061 cacctaattt ttttgcgatt tctatttgct tttcttttct tacgatagca gtgatatttt
     6121 catatccaat tttatgtaaa atttgtatag caacactgcc cacaccacct gtagccccag
     6181 acactaaaat ttcaggttgt gatttgatat tcattccata tttttctagt tcaaaaattg
     6241 ataatgctgc cgtaaaaccg gctgtgccat ataccataga ctctttaata gacatttctt
     6301 ggggaatagg aacaatccac tctccaggca cttgtgcata ttctgaaaat ccacctgtat
     6361 gtgtcacgcc aacatcataa ccagtaacta atacattatc gccttcttta aacttatcat
     6421 tcgaacttga ttcaacaata cctgcaaaat ctataccagg aatcatagga taatctctaa
     6481 ttacaccgcc tttatattgt aaagctagca tatccttata atttattgaa gagtatacca
     6541 cttttactaa tacatcaccc ttcgataaat gagattcatc aactttttct actttataac
     6601 taacatttga tgcacttttt ctcactacta ccgcattgaa catttattag ccttctttca
     6661 caattgtttt gtagaagtat gtttttgttg caaaactatt ttcctacaaa acttatcatg
     6721 atatgataag cgagtcaata ctaatgattt aagagaggtt ttagttgtgg aattagataa
     6781 ttgtataaat tatttgttga gtacttctca gaatagagtt tttaaacatt ttaaaagttt
     6841 attaagtccc tataatatta ctccagcaga atatggtgtt ttgaattgct tgattaatag
     6901 taatttgaat acacctaaag cgattggtaa tactttaaat ttagaacctt caacaatatc
     6961 tggaatatta gataagatgt ccaaaaagga tctaataaca cgtcaaactg ataatgaaaa
     7021 tagaagaact gtgctaataa acagtacaaa taaagcaaaa gacttatggc caacattaga
     7081 aaagaaagcc tatcaactta ataatcatta cttcgctaat ttaactaatg aagaaactgt
     7141 agtgtttaaa aagatattac taaagataaa taaaactacg ttttaatttt tacgttataa
     7201 aaaagttgaa gtgcgaatta taatcgagta aatataaatg atagcttagc ttaattatat
     7261 attaaagcca actatacttt tattcccgaa aaacctacta tccaaatttc taagatactt
     7321 tagcctctat aataaacata ttgtaatata atttatgtat tatttaggtt taccctaatc
     7381 gaacaataaa aaatacttgt aaaatgctat tgtcactttg cttgatatat gagaattatt
     7441 taaccttata aatgagaaaa aagcaacgca ctttaaataa gatacgttgc tttttcgatt
     7501 gatgaacacc tataattaaa ctattcatct attatttatg attttttgta tatacaatat
     7561 ttctagtttg ttaaagagaa ttaagaaaat aaatctcgaa aataataaag ggaaaatcag
     7621 tttttgatat caaaattata catgtcaacg ataatacaaa atataataca aactataaga
     7681 tgttatcagt atttattatg catttagaat attaaaattc cttcattaca ctcttggcgg
     7741 tttcacttat caacttatca tttggcttat cacttttatt gtctttattc gtaaaaatga
     7801 ctaaaacaat aggttcagat tggcccttag gataaacaaa agcaacatca tttctagaag
     7861 catatgttat tgcttgacca cttttatcag caaccttata gtcttttgga acaccatctt
     7921 taattaaagt gtctccgttt ttattattaa acattaaatc aagtaagaaa tttttatttt
     7981 ttttgcttaa ttttccattt gcgataagtt tatttaaagt cttgccgaaa gcagcaggcg
     8041 ttgaagtatc ttttttgctc tttggtgaat agtaatttaa ttctatctca tatctaactg
     8101 gatttgttac tttatctcct agttctttta gacgttgttt aacttttttg attccaccga
     8161 tttcttttat aattttattg tttgctgtat tatcactata tgtcattgaa gcctcaataa
     8221 gttcttttaa agtgatatct tttcctacat atttttctaa aataggagaa taagcaacta
     8281 tatcatcttt gttaatatgt acttttttat ttaacttatt ataaggtact tgttctaaca
     8341 aaatagcact atttatcgct tttgaagtcg aagcataggc aaatctctta tctgaattaa
     8401 attttacttc cttaccactt ttagtattta aagcataaac accaatatga gcattatatt
     8461 ttttttctaa atcatttaac tctttggcat gtgaactgtt tgaattacat gcacttaaaa
     8521 ctaaagcaat tacaattaaa aatattaact ttttcaaaat aaaccctccg atattacagt
     8581 tgtaattttt ataaatcaat aatattacaa ttgtaatatt gatgtcaatt gtttattata
     8641 aatatttgaa agaaggtttc aaaatggcta aattactaat aatgagcgta gtaagctttt
     8701 gttttatatt tctattgtta gtatttttta ggtacatatt aaaacgctat tttaattata
     8761 gtttaaatta taaagtttgg tatctaacta tgcttgctgg attaattcct tttatcccaa
     8821 ttaaattctc ttttataaaa tttaataatg tgaataatca agcgcccaca gttgaaagta
     8881 agtcacacga tttgaaccat aacataaata ccaccaaacc tattcaagag ttcacaacag
     8941 atatccataa gtttaattgg gattcaattg ataatatctg cacagttatt tggatagttt
     9001 tagttattat tttaagtttt aaatttttga aagccttatt atatcttaaa tatttaaaga
     9061 aacagtcact ttatctaaac gaaaatgaaa aaaataaaat agatacgata cttttcaacc
     9121 atcaatataa aaaaaatatt gtgattcgaa aagcagaggc tattcaatct ccaataactt
     9181 tttggtatgg gaaatatatt attttgattc ctagttcata ttttaaaagt gtaattgata
     9241 aaagactaaa atatataata ctgcatgaat atgctcatgc taaaaataga gatactttac
     9301 atttaattat ctttaatatc ttctctatta ttatgagtta caatccatta gtacatattg
     9361 taaaaagaaa gataattcat gacaatgaag tagaagccga tagatttgtc cttaataata
     9421 ttaacaaaaa tgaatttaaa acttatgcgg aatctattat ggactccgta ttaaaaaccc
     9481 cattttccaa caaaaatata ttaagccatt catttaatgg taaaaaatca ttactcaaaa
     9541 gtagattaat taatataaaa gaagccgatc tgaaaaagca gtcaaaatta attctaattt
     9601 ttatttgtat ttttactttt tttatcatga taattcaaag ccagttttta atggggcaat
     9661 ccttaactga ttataattat aaaaagccct tacaaagcga ttaccaaatt ttagatgaaa
     9721 gtaaaaattt tggttcaaat agtggatctt tcgttatgta cagcatgaag aaagacaaat
     9781 actatattta taatgaaaaa gaaagtagaa aaagatattc tcctgattca acttacaaaa
     9841 tttatttagc actgtttgga cttgaccgtc atattataag tgataaaaat tcacgtatgt
     9901 catggaatca taaacattat cttttcgagt cttggaataa ggaacaagat ttaaatacag
     9961 caatgcaaaa ttcagttaat tggtacttcg aacgtattag taatcaaata ccaaagaact
    10021 atactgcggc tcaactcaag caattaaatt atggtaatga aaatttggga agttataaaa
    10081 gctattggat ggaagatagt ttgaaaatat ctaatcttga acaagtaata gtttttaaaa
    10141 atatgatgga acaaaataac cattttagta aaaaagcaaa gaatcaatta tcttcttcat
    10201 tattgattaa gaaaaatgaa aagtatgaac tgtatgggaa aacaggtaca ggtatagtaa
    10261 acgggaagta taataatggg tggtttgtag gttacgtaat tacaaatcat gataagtatt
    10321 attttgctac acatttatca gatggaaacc cgtctgggaa aaatgctgaa ttaataagtg
    10381 aaaaaatatt aaaaggaatg ggtgttttaa atgaccaata agcaagttga aatatctatg
    10441 gctgaatggg atgttatgaa tataatatgg gataaaaaat cagtatcagc taatgaaatt
    10501 gtagttgaaa ttcaaaaata taaagaagtt agtgataaaa cgattagaac attaatcaca
    10561 agactatata aaaaagagat tataaaacga tacaaatcag aaaatattta tttttactca
    10621 tcaaatatta aagaagacga tattaaaatg aaaactgcta aaacctttct taataaactg
    10681 tatggagggg atatgaaaag tttagtgctg aattttgcga aaaatgaaga attaaataac
    10741 aaagaaattg aagaattgcg agacatttta aatgatatta gtaaaaagta attagaggta
    10801 tgtttcaaac ataatacata tgtaaaccat acttgactct tattaaatta gttttgttaa
    10861 tgacatttaa aatatattat taaagatgag agtgtccaga aaataaccat tttctgtaca
    10921 ctcttgtttt ttgtctaaat aagtaagtat tacatagaat tatgtgttca aaaacccaac
    10981 aaaaaatcta tgttcacaaa agacctcgta atcaagttat ggtacactaa aggtgtatat
    11041 aggaggtctg tattttgaaa ataggttatg caagagtttc aactggatta caaaatttga
    11101 atttacagga ggatcgatta aatacatatg gttgtgaaaa gatatttaat gaccatatga
    11161 gtggttcaaa aagtaagcga cctggtttag ataaagcaat tgaatttgcg aggtcaggag
    11221 atacgattgt tgtttggaga ttagatagac taggacggaa catggaggat ttaatcacgt
    11281 tagttaatga acttaacgaa cgaggtgtga gttttcatag tttagaagaa aatataacga
    11341 tggataaatc aagttctaca ggacaattat tatttcattt attcgcagca tttgcagagt
    11401 ttgaacgtaa tttaatttta gagcgatcat cagccggtcg aattgcagca cgtgctagag
    11461 gccgatatgg aggtagacct gaaaaattaa ataaacaaga tttaaactta cttaagacac
    11521 tttatgataa tggtacgcca attaaaacaa ttgcagagca gtggcaagtt tcacgtacaa
    11581 ctatttatcg ttatctcaat aaattagagg caaaagagaa taaaaacaag tagagcaact
    11641 aatctagctt actataatga atctactaaa taattttata tatcattaac atacaaagac
    11701 tttgattcaa tgtcactcga tgtaaaagag acgttctgac agcgtctaaa atctcataat
    11761 attttcctgt tatgagattt taataataac acaagaaaaa agcgtacaaa ttcgtacgct
    11821 tttttaatat aatacctgtt tcactaatga acgtttataa cgcaatcatg atgagagtat
    11881 taatacagtt aattatttga aatactttag tttacataat cgatgattac cagaagttct
    11941 cactatataa aagacactaa aattttataa cttttagtgc ctaatcaatt attttagcta
    12001 ccaaacaaac gactccaaaa ccccttttta ggttccgttt cttcactctt ttcttctata
    12061 tcactatcat tgtcatttag attagctgga acgtcttctt tttttgcttc ttcaccttgt
    12121 gtactttcgt cgctttgact tccagatata tccttatatt gaatctctgt ttctttagtt
    12181 tcttcctgat ctctgttaat atttacagga ttagtagtat atgttttttc ttgtgcatca
    12241 acgttttgtc tagtgttagt agatgtatca aagggataat taaggtgttt ttctttttct
    12301 aattgatttt ctaatttttg aatcttttta ttactctcta atgccagaac ttgctgattt
    12361 tcaagcaatt ttgatctttc ttgcaactct tttctgtaaa attcaattgt tgtttcatga
    12421 cgactttctt gtttttcaat ttgattattt aaagtatcaa tctgattttt aaggatttct
    12481 actatttgag catcgctttt cgattcaaaa ttaatattat ccgacccttt tttttgtttg
    12541 tcttcagtag tactttcaaa accatatttc tttttatttt catttactct ttgaatgatt
    12601 ttttctatat cggtatcttc tttaataaac gaagtgtttt cctgttttat tgtctctata
    12661 ttcaatcgct taatattatt aaaaatcgtt tgcttactaa cccctaattc ttcactcaac
    12721 tttttcacac ttttcatatc atcaaccctt tataaaatgt ttataaaccc ttgataaaat
    12781 attataagct tataatgctt aataataaag tgattaataa ataaaaataa acatccatca
    12841 acctttttat caagggttta tgaaaggttt atgatattta tcaagggttt ataaaccctt
    12901 gataaagcta tattttccct tttataagtt tatttaaagt gctataataa acttaatata
    12961 acaaaaaccc caactactgg taatagtcgg ggtttggtaa taaaacgttt agaaactgtt
    13021 tatttatata attttcgata cttgtattat ataatgaata atatcttagt gcaagcacct
    13081 aaatcattaa ataaatgtaa agctaaataa aaacgtttta ttacctctta ccaaactaaa
    13141 atatggaaag aggttttttg tca
//
