LOCUS       CP030535               13163 bp    DNA     circular BCT 10-OCT-2019
DEFINITION  Staphylococcus aureus strain ER00749.3 plasmid unnamed1, complete
            sequence.
ACCESSION   CP030535
VERSION     CP030535.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN09483929
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Sullivan,M.J., Altman,D.R., Chacko,K., Cliferri,B., Webster,E.,
            Deikus,G., Lewis,M., Khan,Z., Beckford,C., Rendo,A., Samaroo,F.,
            Sebra,R., Karam-Howlin,R., Southwick,K., Adams,E., Ying,L.,
            Kornblum,J., Factor,S., Danesh Yazdi,M., Dingle,T., Hamula,C.,
            Bashir,A., Schadt,E., Kasarskis,A., Patel,G., Wallach,F., Gibbs,K.
            and van Bakel,H.
  TITLE     Whole genome sequencing to identify and define MRSA outbreaks
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13163)
  AUTHORS   Sullivan,M.J., Altman,D.R., Chacko,K., Cliferri,B., Webster,E.,
            Deikus,G., Lewis,M., Khan,Z., Beckford,C., Rendo,A., Samaroo,F.,
            Sebra,R., Karam-Howlin,R., Southwick,K., Adams,E., Ying,L.,
            Kornblum,J., Factor,S., Danesh Yazdi,M., Dingle,T., Hamula,C.,
            Bashir,A., Schadt,E., Kasarskis,A., Patel,G., Wallach,F., Gibbs,K.
            and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2018) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 172.746906802x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2018 17:43:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,911
            CDS (total)                       :: 2,829
            Genes (coding)                    :: 2,728
            CDS (coding)                      :: 2,728
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 44 of 101
            Pseudo Genes (incomplete)         :: 33 of 101
            Pseudo Genes (internal stop)      :: 37 of 101
            Pseudo Genes (multiple problems)  :: 13 of 101
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ER00749.3"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2015"
                     /collected_by="Mount Sinai Hospital"
     gene            join(12785..13163,1..2)
                     /gene="blaI"
                     /locus_tag="DQU48_14485"
     ARG             12786..13162
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            266..844
                     /locus_tag="DQU48_14490"
     CDS             266..844
                     /locus_tag="DQU48_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="QFK99167.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="HTH domain-containing protein"
     gene            complement(1205..1948)
                     /locus_tag="DQU48_14495"
     CDS             complement(1205..1948)
                     /locus_tag="DQU48_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="QFK99168.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     gene            2374..3357
                     /locus_tag="DQU48_14500"
     CDS             2374..3357
                     /locus_tag="DQU48_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="QFK99169.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication protein"
     gene            3350..3666
                     /locus_tag="DQU48_14505"
                     /pseudo=""
     CDS             3350..3666
                     /locus_tag="DQU48_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /name="replication protein"
     gene            complement(4530..5222)
                     /locus_tag="DQU48_14510"
     CDS             complement(4530..5222)
                     /locus_tag="DQU48_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QFK99170.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="hypothetical protein"
     gene            5332..5526
                     /locus_tag="DQU48_14515"
     CDS             5332..5526
                     /locus_tag="DQU48_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QFK99171.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            6642..7259
                     /locus_tag="DQU48_14520"
     CDS             6642..7259
                     /locus_tag="DQU48_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium transporter"
                     /protein_id="QFK99172.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQYL
                     GSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNEKG
                     LSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQKLAN
                     IPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
                     /name="cadmium transporter"
     gene            7278..7625
                     /locus_tag="DQU48_14525"
     CDS             7278..7625
                     /locus_tag="DQU48_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="QFK99173.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="transcriptional regulator"
     gene            7782..7874
                     /locus_tag="DQU48_14530"
     CDS             7782..7874
                     /locus_tag="DQU48_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="QFK99174.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(8046..9017)
                     /locus_tag="DQU48_14535"
     CDS             complement(8046..9017)
                     /locus_tag="DQU48_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="QFK99175.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="NADPH:quinone reductase"
     gene            9141..9560
                     /locus_tag="DQU48_14540"
     CDS             9141..9560
                     /locus_tag="DQU48_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="QFK99176.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            complement(9782..9877)
                     /locus_tag="DQU48_14545"
                     /pseudo=""
     CDS             complement(9782..9877)
                     /locus_tag="DQU48_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560862.1"
                     /note="incomplete; partial on complete genome; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /name="O-antigen ligase domain-containing protein"
     gene            complement(10086..10931)
                     /locus_tag="DQU48_14550"
     CDS             complement(10086..10931)
                     /locus_tag="DQU48_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="QFK99177.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALNT
                     KSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEKYV
                     GKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIELNYY
                     SPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPKDYKV
                     ADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKSVMKEF
                     "
                     /name="penicillin-hydrolyzing class A beta-lactamase BlaZ"
     gene            11038..12795
                     /locus_tag="DQU48_14555"
     CDS             11038..12795
                     /locus_tag="DQU48_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="QFK99178.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTMLA
                     GLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSIDN
                     ICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIVIRK
                     AEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFNIFSI
                     IMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSNKNILS
                     HSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSLTDYNYK
                     KPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYKIYLALFG
                     LDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIPKNYTAAQL
                     KQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQLSSSLLIKK
                     NEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGKNAELISEKIL
                     KGMGVLNDQ"
                     /name="beta-lactam sensor/signal transducer BlaR1"
     ARG             11039..12792
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     ARG             complement(10090..10931)
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             6643..7256
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(10087..10931)
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          3723..3870
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.980000000000001e-72"
     Typing          complement(1206..1948)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2375..3357
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3489..3658
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.605"
                     /coverage="91.0"
                     /evalue="1.04e-65"
ORIGIN
        1 aattagaggt atgtttcaaa cataatacat atgtaaacca tacttgactc ttattaaatt
       61 agttttgtta atgacattta aaatatatta ttaaagatga gagtgtccag aaaataacca
      121 ttttctgtac actcttgttt tttgtctaaa taagtaagta ttacatagaa ttatgtgttc
      181 aaaaacccaa caaaaaatct atgttcacaa aagacctcgt aatcaagtta tggtacacta
      241 aaggtgtata taggaggtct gtattttgaa aataggttat gcaagagttt caactggatt
      301 acaaaatttg aatttacagg aggatcgatt aaatacatat ggttgtgaaa agatatttaa
      361 tgaccatatg agtggttcaa aaagtaagcg acctggttta gataaagcaa ttgaatttgc
      421 gaggtcagga gatacgattg ttgtttggag attagataga ctaggacgga acatggagga
      481 tttaatcacg ttagttaatg aacttaacga acgaggtgtg agttttcata gtttagaaga
      541 aaatataacg atggataaat caagttctac aggacaatta ttatttcatt tattcgcagc
      601 atttgcagag tttgaacgta atttaatttt agagcgatca tcagccggtc gaattgcagc
      661 acgtgctaga ggccgatatg gaggtagacc tgaaaaatta aataaacaag atttaaactt
      721 acttaagaca ctttatgata atggtacgcc aattaaaaca attgcagagc agtggcaagt
      781 ttcacgtaca actatttatc gttatctcaa taaattagag gcaaaagaga ataaaaacaa
      841 gtagagcaac taatctagct tactataatg aatctactaa ataattttat atatcattaa
      901 catacaaaga ctttgattca atgtcactcg atgtaaaaga gacgttctga cagcgtctaa
      961 aatctcataa tattttcctg ttatgagatt ttaataataa cacaagaaaa aagcgtacaa
     1021 attcgtacgc ttttttaata taatacctgt ttcactaatg aacgtttata acgcaatcat
     1081 gatgagagta ttaatacagt taattatttg aaatacttta gtttacataa tcgatgatta
     1141 ccagaagttc tcactatata aaagacacta aaattttata acttttagtg cctaatcaat
     1201 tattttagct accaaacaaa cgactccaaa accccttttt aggttccgtt tcttcactct
     1261 tttcttctat atcactatca ttgtcattta gattagctgg aacgtcttct ttttttgctt
     1321 cttcaccttg tgtactttcg tcgctttgac ttccagatat atccttatat tgaatctctg
     1381 tttctttagt ttcttcctga tctctgttaa tatttacagg attagtagta tatgtttttt
     1441 cttgtgcatc aacgttttgt ctagtgttag tagatgtatc aaagggataa ttaaggtgtt
     1501 tttctttttc taattgattt tctaattttt gaatcttttt attactctct aatgccagaa
     1561 cttgctgatt ttcaagcaat tttgatcttt cttgcaactc ttttctgtaa aattcaattg
     1621 ttgtttcatg acgactttct tgtttttcaa tttgattatt taaagtatca atctgatttt
     1681 taaggatttc tactatttga gcatcgcttt tcgattcaaa attaatatta tccgaccctt
     1741 ttttttgttt gtcttcagta gtactttcaa aaccatattt ctttttattt tcatttactc
     1801 tttgaatgat tttttctata tcggtatctt ctttaataaa cgaagtgttt tcctgtttta
     1861 ttgtctctat attcaatcgc ttaatattat taaaaatcgt ttgcttacta acccctaatt
     1921 cttcactcaa ctttttcaca cttttcatat catcaaccct ttataaaatg tttataaacc
     1981 cttgataaaa tattataagc ttataatgct taataataaa gtgattaata aataaaaata
     2041 aacatccatc aaccttttta tcaagggttt atgaaaggtt tatgatattt atcaagggtt
     2101 tataaaccct tgataaagct atattttccc ttttataagt ttatttaaag tgctataata
     2161 aacttaatat aacaaaaacc ccaactactg gtaatagtcg gggtttggta ataaaacgtt
     2221 tagaaactgt ttatttatat aattttcgat acttgtatta tataatgaat aatatcttag
     2281 tgcaagcacc taaatcatta aataaatgta aagctaaata aaaacgtttt attacctctt
     2341 accaaactaa aatatggaaa gaggtttttt gtcatgcaaa atcaatattt tacagtgcaa
     2401 gaaaattaca aagaaagatt ttatcaaatc cctaaagtat tctttaccag tgaaaattat
     2461 aaaaatctaa ctaatgatat gaaaatagcc tatgctatct tacgtgatcg cttaaattta
     2521 tctattaaaa atagctgggt tgatgaagat ggtaacattt actttgttta ttcaaatgaa
     2581 aagctaatgg aaatcttaaa ttgtaaaaaa gaaaagttaa caaaaataaa aaaagggtta
     2641 gaaaacgatg gtctactcat tcaaaaacga cgtggtttaa ataaaccaaa tattctatat
     2701 ttaatgaaac ctattgtcac agaacgtgat atttacaaaa tagaaaaaga agaaaacgat
     2761 gttgaacctt acggcgagaa ggaagttcga aaatcgaacg tccagaagtt cgaaaatcga
     2821 acgtccagaa gttcgaaaat cgaacgtcca gaagttcgaa aatcgaacac taatgatact
     2881 gactttaatg acattgactt taatgatact gagaataatg atatgaatga cttgaatgat
     2941 ataaagtaca aaaatgaaat tagtaatcat tcaaatcatt caaatcaatt tacacataat
     3001 tttgatgata aagaaatgct attgcaagaa ttcccagaac aactaactaa ttatttatta
     3061 aaatatgatt atagagattt agaaatcata aaggctgtta ttcttaaagc aaaaaaatca
     3121 tttaattcaa ggcatgaaga tatgcattac atgttagagg acattgagga tgaaatatta
     3181 acttctttaa aaagacttaa aaaagctata catgataggg gagttaaagg ccaaaaggaa
     3241 acaatcaagt caatgcaagc ttatttaatg caaacaattc taactgaact tgaagaaaca
     3301 catgcccttt atatgcgccg taaaaacatg aaacaatata atatctttaa tcaataattc
     3361 aaatgatttt ataacactcg aagagacatc aaaatattcc gttaaatcat ctgacttttt
     3421 aactgatgac gacgaaaaaa atcaagaaat tgtaagtgat ttagaaaaag gtttgtatcg
     3481 aaaacgtatg ttaagttatg gtggattgct taaacaaaag cataaaattt taaacttaga
     3541 cgatgccgaa gatggtaatt tgattaatac aagtgatgaa gataaaacga cagacgaaga
     3601 aaaaaagcac attcaattac cgcaatttgg aatttcgaaa agcaaaatta ttatttaaga
     3661 cattagtgat aactgatgtc tttttttatt gtttttctgt aaaaaagtgc tgtcttattt
     3721 ttgtgacaaa tgctgtatgt agtgtcacaa aagtgtgaca ctacagcttt gtatgatatc
     3781 actttaaaat aacttaaaac ccttggaatg tctggccttg ccagatctat catttttgaa
     3841 tgatagcaaa tttcccttat gctcttacgg agattttaga gttaaattaa aatttctaag
     3901 tttttgataa gaaaagccct gcaggaattt agaaaaacat ggggaagttt taaagtattt
     3961 tatgctaatt tttaaatttg catgtaaatg ggcagtatct aaaaaattag tcactgattt
     4021 tgctgaaatt tttgtttgtg aaattagcat atatttattt ggctcatatt tgcgttttaa
     4081 gagactgtat agatttttaa gtacaaaacc tatatgattt gcctctaagt cttcaaaatg
     4141 cctcttaaaa tccaaaataa aagtgtttaa tattgtaata aggattgcta tttttataaa
     4201 aggagagata tcatgaagat taaaactttc gctactttta ctcatcgtca attagataaa
     4261 caagttaata ggtttttaaa gaatgatatt catgtattag aaattcaaac acacagtaat
     4321 tggttatata tttcagctat tgttttttat gaggataaat aaggaacatc gtatttatgc
     4381 cgaaaaaatt tattgatgtt gagaagaacc cttaactaaa cttgcagacg aatgtcggca
     4441 tagcgtgagc tattaagccg accattcgac aagttttggg attgttaagg gttccgaggc
     4501 tcaacgtcaa taaaacaatt ggaataaaat caacctacag agcatccagc tgcaaatgca
     4561 gttccgcaaa ctgcttcaca tacaccgccg gctattggat ttacgattgc ccatactcca
     4621 cagtagtgag cacatgcata aagtccagcc atactacaag cgaatgcttt gccgttccat
     4681 ttgaatcctg catatgttac cggtctgttt ttatcaactt tttcagcctt atattcaacg
     4741 taatttttgt taataagtat ttttccctct ttgttttcag tcatacttct tttttcggca
     4801 atgaattttg tgattttttt gttgtagtta tcatatattg tttcagaaac tacaatttct
     4861 tttgtttttt catttttata agcgttatta gctatgtagc catatgcatt tttatcattt
     4921 gcttctgtag ctagatattt taatttagaa tttttgtctt ctagctttat ttttttaaat
     4981 ccttcttttt tcatttgctt ttcgaatttt tgcatatctt catttttatt ttgcaattgt
     5041 tctagaaaac ttatttcttt cttggtcatt tgtgatactt cgatgttttt gcttttcaat
     5101 ttttcttttt gattttctag taaagaactt ttttgttctt catttgcgta agcattagaa
     5161 aaattcgcgc taaataccag taggaacaag gaaattattg aaaataattt tataattttc
     5221 atttgatacc tccaagataa taagatattt taattgtata agtaagcgct ttataaaatc
     5281 aatatttatt ttaatatatt tataaacata tttttgaaag gagtctattt tatgaatgaa
     5341 aatttagaaa aatatataaa aagtttgcca cttttaggtt tgttaattag tattttgtta
     5401 attgttttat tcttttttat tttagatgtt gatgaagatt tattcgtggt ttttttatac
     5461 tgtttattgc ctttgatagt taatttgtct atttatggtt tttatgtttt tgttaaaaga
     5521 aagtaagatt atgaataatt ttgtttttaa tttgtatgct ttgagggggt tggaaaacgg
     5581 taggtgtata gattagattt cttgtttttt tgaaacgagt gtgaacgagt ttcttcttgt
     5641 cttgattttt gataaaaacg ccctacagga atttagaaaa acgtggggaa gttttaaaga
     5701 attttatgct aatttttaat ttgcatgtaa atgggcagtg tctaaaaata gttattgttt
     5761 ttgctcaaat ttttgttttt gaaaatagca tatatttatt tggctcatat ttgcgtttta
     5821 aaagcatata taagttttag atataaaact atatgaatca cccctaaatc tttaaaatgc
     5881 cctcttaaaa ttcaaaataa gggcataaaa aataaaagct accccagata aagtgaggta
     5941 gctttttctt tcatacggtc tatttatttt tctttcgacg ccgtatcgtc gatgtaattc
     6001 aaaaaagtta tgggctataa atctacatca ctttttcaag aatgtagtag caatattcaa
     6061 cgcaaattaa tttttataac tgctcggaat atctcaagcc gtttctttaa ttttgaaata
     6121 aaaaaatcga cgaaggtcga ttacgttttt gcacatgtcg atttatccga cgtataagtg
     6181 cgcccttacg ggatttaact agattataac gacgaatttt agacctgtaa agcaataaca
     6241 attaggtcta ccaggtagat aaagtgtaca aagtagaacc gaaaaagtta tttattttga
     6301 agatttttcg tagatataat ttgctaataa acttcctaaa actgaataaa gaaaaatagt
     6361 taaactcatg taatcactcc ataattctga agtgtctcca tgtctacgca tagacacgca
     6421 tttatgccga aaaaatttat tgatgttgag aagaaccctt aactaaactt gcagacgaat
     6481 gtcggcatag cgtgagctat taagccgacc attcgacaag ttttgggatt gttaagggtt
     6541 ccgaggctca acgtcaataa agcaattgga ataaagcacc tatgattatt taaaaatata
     6601 agttataata cattcaaaaa tatttttgaa tgaggtgtat tatgattcaa acggttgttg
     6661 ctgctgctgt tctttatatt gctacagcag ttgatttatt agtaatttta ttaatatttt
     6721 ttgctagagc aaagactaga aaagaatata gagatattta tgttggtcaa tatttagggt
     6781 ctattatttt aatattagtt agtttgtttt tagcttttgt attaaattat gttccagaaa
     6841 agtggatatt aggtttattg ggtttaatac caatttatct tggaattaaa gtggctattt
     6901 atgatgattg tgaaggagaa aagagagcta aaaaagaatt gaatgaaaaa ggattgtcta
     6961 aattagttgg tacggttgca attgttacga tagcaagttg tggcgccgat aatattggtt
     7021 tatttgttcc gtattttgtg acattaagtg ttactaattt attacttact ctgtttgtct
     7081 ttttaatttt aattttcttc ttggtgttta ctgcacaaaa attagctaat attccaggaa
     7141 ttggagaaat tgttgagaaa tttagtcgtt ggattatggc tattatttat atagctttag
     7201 gtttatttat tattattgaa aatgacacta ttcaaacaat tttaggattt atattttaat
     7261 ttagggtgtg attttatatg agttatgaaa atacttgtga tgtgatctgt gtacatgagg
     7321 ataaagttaa caatgcttta agttttttag aagatgataa atctaagaaa ttacttaaca
     7381 ttttagaaaa aatttgtgat gagaagaaat tgaaaatcat attatctttg attaaagaag
     7441 atgagttgtg tgtttgtgat atttctttga tattgaaaat gagtgttgct tcaacttcac
     7501 atcatttaag gcttttatat aaaaatgagg tacttgattt ttataaagat ggaaagatgg
     7561 catattattt tattaaagac gatgaaataa gagagttttt ctctaaaaat catgagggtt
     7621 tttgaaacga gtttcttctt gtcttgatac tataaaaaac aaagttatat aacataagac
     7681 gacttatcgg aagtagatat gcttgtatat tcacagatgg tataataata gagtcgccta
     7741 tctctcaggc gtcaattgac gaagagagga ggtgcattac tttgctaatc attttcgttc
     7801 acatcatagc accagtcatc agtggctgtg ctgttgcgta ttatacttat tggcttagta
     7861 aacgcaacaa ataattaata ggcgacttta taagccagca tcgaaaaaat ccccacacta
     7921 ctgcaatagt gtggggattg gtgcattaat tgttaatcat tttcgttata taaattataa
     7981 cactggttag tagagaaatg caaaatgatt gaattaatag taaatgtatt aatgaatact
     8041 cacacttata cttttaaaat agttcttcct aaatgttgtc cttttaaaag ctgatctatc
     8101 gttttataga attcttcaaa tccaatttca ttgtatagaa gtgaatttcc tatattccat
     8161 tcatttgcta acttactcca tatatcttca cggatattat gatcaacata tacagaatca
     8221 atgccaatta atttaatacc tctaagaata aacggtaata tattagcgtt aagtgaattt
     8281 cctgctacat ttccacatac agtagcaaca ccttgataat ttagacgttt taaaccgtat
     8341 aatgttacat tactccccac tgtatctaag aaataatcaa aaataccttt tttaagcggt
     8401 ttattatcat cttcaattat tattacttgt tttgcaccta atttttttgc gatttctatt
     8461 tgcttttctt ttcttacgat agcagtgata ttttcatatc caattttatg taaaatttgt
     8521 atagcaacac tgcccacacc acctgtagcc ccagacacta aaatttcagg ttgtgatttg
     8581 atattcattc catatttttc tagttcaaaa attgataatg ctgccgtaaa accggctgtg
     8641 ccatatacca tagactcttt aatagacatt tcttggggaa taggaacaat ccactctcca
     8701 ggcacttgtg catattctga aaatccacct gtatgtgtca cgccaacatc ataaccagta
     8761 actaatacat tatcgccttc tttaaactta tcattcgaac ttgattcaac aatacctgca
     8821 aaatctatac caggaatcat aggataatct ctaattacac cgcctttata ttgtaaagct
     8881 agcatatcct tataatttat tgaagagtat accactttta ctaatacatc acccttcgat
     8941 aaatgagatt catcaacttt ttctacttta taactaacat ttgatgcact ttttctcact
     9001 actaccgcat tgaacattta ttagccttct ttcacaattg ttttgtagaa gtatgttttt
     9061 gttgcaaaac tattttccta caaaacttat catgatatga taagcgagtc aatactaatg
     9121 atttaagaga ggttttagtt gtggaattag ataattgtat aaattatttg ttgagtactt
     9181 ctcagaatag agtttttaaa cattttaaaa gtttattaag tccctataat attactccag
     9241 cagaatatgg tgttttgaat tgcttgatta atagtaattt gaatacacct aaagcgattg
     9301 gtaatacttt aaatttagaa ccttcaacaa tatctggaat attagataag atgtccaaaa
     9361 aggatctaat aacacgtcaa actgataatg aaaatagaag aactgtgcta ataaacagta
     9421 caaataaagc aaaagactta tggccaacat tagaaaagaa agcctatcaa cttaataatc
     9481 attacttcgc taatttaact aatgaagaaa ctgtagtgtt taaaaagata ttactaaaga
     9541 taaataaaac tacgttttaa tttttacgtt ataaaaaagt tgaagtgcga attataatcg
     9601 agtaaatata aatgatagct tagcttaatt atatattaaa gccaactata cttttattcc
     9661 cgaaaaacct actatccaaa tttctaagat actttagcct ctataataaa catattgtaa
     9721 tataatttat gtattattta ggtttaccct aatcgaacaa taaaaaatac ttgtaaaatg
     9781 ctattgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     9841 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     9901 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     9961 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
    10021 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatgcattta
    10081 gaatattaaa attccttcat tacactcttg gcggtttcac ttatcaactt atcatttggc
    10141 ttatcacttt tattgtcttt attcgtaaaa atgactaaaa caataggttc agattggccc
    10201 ttaggataaa caaaagcaac atcatttcta gaagcatatg ttattgcttg accactttta
    10261 tcagcaacct tatagtcttt tggaacacca tctttaatta aagtgtctcc gtttttatta
    10321 ttaaacatta aatcaagtaa gaaattttta ttttttttgc ttaattttcc atttgcgata
    10381 agtttattta aagtcttgcc gaaagcagca ggcgttgaag tatctttttt gctctttggt
    10441 gaatagtaat ttaattctat ctcatatcta actggatttg ttactttatc tcctagttct
    10501 tttagacgtt gtttaacttt tttgattcca ccgatttctt ttataatttt attgtttgct
    10561 gtattatcac tatatgtcat tgaagcctca ataagttctt ttaaagtgat atcttttcct
    10621 acatattttt ctaaaatagg agaataagca actatatcat ctttgttaat atgtactttt
    10681 ttatttaact tattataagg tacttgttct aacaaaatag cactatttat cgcttttgaa
    10741 gtcgaagcat aggcaaatct cttatctgaa ttaaatttta cttccttacc acttttagta
    10801 tttaaagcat aaacaccaat atgagcatta tatttttttt ctaaatcatt taactctttg
    10861 gcatgtgaac tgtttgaatt acatgcactt aaaactaaag caattacaat taaaaatatt
    10921 aactttttca aaataaaccc tccgatatta cagttgtaat ttttataaat caataatatt
    10981 acaattgtaa tattgatgtc aattgtttat tataaatatt tgaaagaagg tttcaaaatg
    11041 gctaaattac taataatgag cgtagtaagc ttttgtttta tatttctatt gttagtattt
    11101 tttaggtaca tattaaaacg ctattttaat tatagtttaa attataaagt ttggtatcta
    11161 actatgcttg ctggattaat tccttttatc ccaattaaat tctcttttat aaaatttaat
    11221 aatgtgaata atcaagcgcc cacagttgaa agtaagtcac acgatttgaa ccataacata
    11281 aataccacca aacctattca agagttcaca acagatatcc ataagtttaa ttgggattca
    11341 attgataata tctgcacagt tatttggata gttttagtta ttattttaag ttttaaattt
    11401 ttgaaagcct tattatatct taaatattta aagaaacagt cactttatct aaacgaaaat
    11461 gaaaaaaata aaatagatac gatacttttc aaccatcaat ataaaaaaaa tattgtgatt
    11521 cgaaaagcag aggctattca atctccaata actttttggt atgggaaata tattattttg
    11581 attcctagtt catattttaa aagtgtaatt gataaaagac taaaatatat aatactgcat
    11641 gaatatgctc atgctaaaaa tagagatact ttacatttaa ttatctttaa tatcttctct
    11701 attattatga gttacaatcc attagtacat attgtaaaaa gaaagataat tcatgacaat
    11761 gaagtagaag ccgatagatt tgtccttaat aatattaaca aaaatgaatt taaaacttat
    11821 gcggaatcta ttatggactc cgtattaaaa accccatttt ccaacaaaaa tatattaagc
    11881 cattcattta atggtaaaaa atcattactc aaaagtagat taattaatat aaaagaagcc
    11941 gatctgaaaa agcagtcaaa attaattcta atttttattt gtatttttac tttttttatc
    12001 atgataattc aaagccagtt tttaatgggg caatccttaa ctgattataa ttataaaaag
    12061 cccttacaaa gcgattacca aattttagat gaaagtaaaa attttggttc aaatagtgga
    12121 tctttcgtta tgtacagcat gaagaaagac aaatactata tttataatga aaaagaaagt
    12181 agaaaaagat attctcctga ttcaacttac aaaatttatt tagcactgtt tggacttgac
    12241 cgtcatatta taagtgataa aaattcacgt atgtcatgga atcataaaca ttatcttttc
    12301 gagtcttgga ataaggaaca agatttaaat acagcaatgc aaaattcagt taattggtac
    12361 ttcgaacgta ttagtaatca aataccaaag aactatactg cggctcaact caagcaatta
    12421 aattatggta atgaaaattt gggaagttat aaaagctatt ggatggaaga tagtttgaaa
    12481 atatctaatc ttgaacaagt aatagttttt aaaaatatga tggaacaaaa taaccatttt
    12541 agtaaaaaag caaagaatca attatcttct tcattattga ttaagaaaaa tgaaaagtat
    12601 gaactgtatg ggaaaacagg tacaggtata gtaaacggga agtataataa tgggtggttt
    12661 gtaggttacg taattacaaa tcatgataag tattattttg ctacacattt atcagatgga
    12721 aacccgtctg ggaaaaatgc tgaattaata agtgaaaaaa tattaaaagg aatgggtgtt
    12781 ttaaatgacc aataagcaag ttgaaatatc tatggctgaa tgggatgtta tgaatataat
    12841 atgggataaa aaatcagtat cagctaatga aattgtagtt gaaattcaaa aatataaaga
    12901 agttagtgat aaaacgatta gaacattaat cacaagacta tataaaaaag agattataaa
    12961 acgatacaaa tcagaaaata tttattttta ctcatcaaat attaaagaag acgatattaa
    13021 aatgaaaact gctaaaacct ttcttaataa actgtatgga ggggatatga aaagtttagt
    13081 gctgaatttt gcgaaaaatg aagaattaaa taacaaagaa attgaagaat tgcgagacat
    13141 tttaaatgat attagtaaaa agt
//
