LOCUS       CP030521               13163 bp    DNA     circular BCT 10-OCT-2019
DEFINITION  Staphylococcus aureus strain ER01564.3 plasmid unnamed1, complete
            sequence.
ACCESSION   CP030521
VERSION     CP030521.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN09483955
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Sullivan,M.J., Altman,D.R., Chacko,K., Cliferri,B., Webster,E.,
            Deikus,G., Lewis,M., Khan,Z., Beckford,C., Rendo,A., Samaroo,F.,
            Sebra,R., Karam-Howlin,R., Southwick,K., Adams,E., Ying,L.,
            Kornblum,J., Factor,S., Danesh Yazdi,M., Dingle,T., Hamula,C.,
            Bashir,A., Schadt,E., Kasarskis,A., Patel,G., Wallach,F., Gibbs,K.
            and van Bakel,H.
  TITLE     Whole genome sequencing to identify and define MRSA outbreaks
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13163)
  AUTHORS   Sullivan,M.J., Altman,D.R., Chacko,K., Cliferri,B., Webster,E.,
            Deikus,G., Lewis,M., Khan,Z., Beckford,C., Rendo,A., Samaroo,F.,
            Sebra,R., Karam-Howlin,R., Southwick,K., Adams,E., Ying,L.,
            Kornblum,J., Factor,S., Danesh Yazdi,M., Dingle,T., Hamula,C.,
            Bashir,A., Schadt,E., Kasarskis,A., Patel,G., Wallach,F., Gibbs,K.
            and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2018) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 209.286785945x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2018 17:50:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,088
            CDS (total)                       :: 3,006
            Genes (coding)                    :: 2,901
            CDS (coding)                      :: 2,901
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 53 of 105
            Pseudo Genes (incomplete)         :: 36 of 105
            Pseudo Genes (internal stop)      :: 29 of 105
            Pseudo Genes (multiple problems)  :: 13 of 105
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ER01564.3"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2015"
                     /collected_by="Mount Sinai Hospital"
     gene            join(13163,1..845)
                     /locus_tag="DQU74_15140"
     CDS             join(13163,1..845)
                     /locus_tag="DQU74_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="QFL22347.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALNT
                     KSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEKYV
                     GKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIELNYY
                     SPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPKDYKV
                     ADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKSVMKEF
                     "
                     /name="penicillin-hydrolyzing class A beta-lactamase BlaZ"
     gene            1054..1149
                     /locus_tag="DQU74_15145"
                     /pseudo=""
     CDS             1054..1149
                     /locus_tag="DQU74_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560862.1"
                     /note="incomplete; partial on complete genome; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /name="O-antigen ligase domain-containing protein"
     gene            complement(1371..1790)
                     /locus_tag="DQU74_15150"
     CDS             complement(1371..1790)
                     /locus_tag="DQU74_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="QFL22348.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            1914..2885
                     /locus_tag="DQU74_15155"
     CDS             1914..2885
                     /locus_tag="DQU74_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="QFL22349.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="NADPH:quinone reductase"
     gene            complement(3057..3149)
                     /locus_tag="DQU74_15160"
     CDS             complement(3057..3149)
                     /locus_tag="DQU74_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="QFL22350.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(3306..3653)
                     /locus_tag="DQU74_15165"
     CDS             complement(3306..3653)
                     /locus_tag="DQU74_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="QFL22351.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="transcriptional regulator"
     gene            complement(3672..4289)
                     /locus_tag="DQU74_15170"
     CDS             complement(3672..4289)
                     /locus_tag="DQU74_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium transporter"
                     /protein_id="QFL22352.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQYL
                     GSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNEKG
                     LSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQKLAN
                     IPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
                     /name="cadmium transporter"
     gene            complement(5405..5599)
                     /locus_tag="DQU74_15175"
     CDS             complement(5405..5599)
                     /locus_tag="DQU74_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QFL22353.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            5709..6401
                     /locus_tag="DQU74_15180"
     CDS             5709..6401
                     /locus_tag="DQU74_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QFL22354.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="hypothetical protein"
     gene            complement(7265..7581)
                     /locus_tag="DQU74_15185"
                     /pseudo=""
     CDS             complement(7265..7581)
                     /locus_tag="DQU74_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /name="replication protein"
     gene            complement(7574..8557)
                     /locus_tag="DQU74_15190"
     CDS             complement(7574..8557)
                     /locus_tag="DQU74_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="QFL22355.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication protein"
     gene            8983..9726
                     /locus_tag="DQU74_15195"
     CDS             8983..9726
                     /locus_tag="DQU74_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="QFL22356.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     gene            complement(10087..10665)
                     /locus_tag="DQU74_15200"
     CDS             complement(10087..10665)
                     /locus_tag="DQU74_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="QFL22357.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="HTH domain-containing protein"
     gene            complement(10929..11309)
                     /gene="blaI"
                     /locus_tag="DQU74_15205"
     ARG             complement(10933..11309)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            complement(11299..13056)
                     /locus_tag="DQU74_15210"
     CDS             complement(11299..13056)
                     /locus_tag="DQU74_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="QFL22359.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTMLA
                     GLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSIDN
                     ICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIVIRK
                     AEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFNIFSI
                     IMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSNKNILS
                     HSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSLTDYNYK
                     KPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYKIYLALFG
                     LDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIPKNYTAAQL
                     KQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQLSSSLLIKK
                     NEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGKNAELISEKIL
                     KGMGVLNDQ"
                     /name="beta-lactam sensor/signal transducer BlaR1"
     ARG             complement(11303..13056)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     ARG             4..842
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="99.64"
     ARG             complement(3676..4289)
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             4..845
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.79"
                     /coverage="99.64"
     Typing          complement(7062..7209)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.980000000000001e-72"
     Typing          complement(7268..7443)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.697"
                     /coverage="94.0"
                     /evalue="1.04e-65"
     Typing          complement(7575..8557)
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8984..9726
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgaaaaagtt aatattttta attgtaattg ctttagtttt aagtgcatgt aattcaaaca
       61 gttcacatgc caaagagtta aatgatttag aaaaaaaata taatgctcat attggtgttt
      121 atgctttaaa tactaaaagt ggtaaggaag taaaatttaa ttcagataag agatttgcct
      181 atgcttcgac ttcaaaagcg ataaatagtg ctattttgtt agaacaagta ccttataata
      241 agttaaataa aaaagtacat attaacaaag atgatatagt tgcttattct cctattttag
      301 aaaaatatgt aggaaaagat atcactttaa aagaacttat tgaggcttca atgacatata
      361 gtgataatac agcaaacaat aaaattataa aagaaatcgg tggaatcaaa aaagttaaac
      421 aacgtctaaa agaactagga gataaagtaa caaatccagt tagatatgag atagaattaa
      481 attactattc accaaagagc aaaaaagata cttcaacgcc tgctgctttc ggcaagactt
      541 taaataaact tatcgcaaat ggaaaattaa gcaaaaaaaa taaaaatttc ttacttgatt
      601 taatgtttaa taataaaaac ggagacactt taattaaaga tggtgttcca aaagactata
      661 aggttgctga taaaagtggt caagcaataa catatgcttc tagaaatgat gttgcttttg
      721 tttatcctaa gggccaatct gaacctattg ttttagtcat ttttacgaat aaagacaata
      781 aaagtgataa gccaaatgat aagttgataa gtgaaaccgc caagagtgta atgaaggaat
      841 tttaatattc taaatgcata ataaatactg ataacatctt atagtttgta ttatattttg
      901 tattatcgtt gacatgtata attttgatat caaaaactga ttttcccttt attattttcg
      961 agatttattt tcttaattct ctttaacaaa ctagaaatat tgtatataca aaaaatcata
     1021 aataatagat gaatagttta attataggtg ttcatcaatc gaaaaagcaa cgtatcttat
     1081 ttaaagtgcg ttgctttttt ctcatttata aggttaaata attctcatat atcaagcaaa
     1141 gtgacaatag cattttacaa gtatttttta ttgttcgatt agggtaaacc taaataatac
     1201 ataaattata ttacaatatg tttattatag aggctaaagt atcttagaaa tttggatagt
     1261 aggtttttcg ggaataaaag tatagttggc tttaatatat aattaagcta agctatcatt
     1321 tatatttact cgattataat tcgcacttca acttttttat aacgtaaaaa ttaaaacgta
     1381 gttttattta tctttagtaa tatcttttta aacactacag tttcttcatt agttaaatta
     1441 gcgaagtaat gattattaag ttgataggct ttcttttcta atgttggcca taagtctttt
     1501 gctttatttg tactgtttat tagcacagtt cttctatttt cattatcagt ttgacgtgtt
     1561 attagatcct ttttggacat cttatctaat attccagata ttgttgaagg ttctaaattt
     1621 aaagtattac caatcgcttt aggtgtattc aaattactat taatcaagca attcaaaaca
     1681 ccatattctg ctggagtaat attataggga cttaataaac ttttaaaatg tttaaaaact
     1741 ctattctgag aagtactcaa caaataattt atacaattat ctaattccac aactaaaacc
     1801 tctcttaaat cattagtatt gactcgctta tcatatcatg ataagttttg taggaaaata
     1861 gttttgcaac aaaaacatac ttctacaaaa caattgtgaa agaaggctaa taaatgttca
     1921 atgcggtagt agtgagaaaa agtgcatcaa atgttagtta taaagtagaa aaagttgatg
     1981 aatctcattt atcgaagggt gatgtattag taaaagtggt atactcttca ataaattata
     2041 aggatatgct agctttacaa tataaaggcg gtgtaattag agattatcct atgattcctg
     2101 gtatagattt tgcaggtatt gttgaatcaa gttcgaatga taagtttaaa gaaggcgata
     2161 atgtattagt tactggttat gatgttggcg tgacacatac aggtggattt tcagaatatg
     2221 cacaagtgcc tggagagtgg attgttccta ttccccaaga aatgtctatt aaagagtcta
     2281 tggtatatgg cacagccggt tttacggcag cattatcaat ttttgaacta gaaaaatatg
     2341 gaatgaatat caaatcacaa cctgaaattt tagtgtctgg ggctacaggt ggtgtgggca
     2401 gtgttgctat acaaatttta cataaaattg gatatgaaaa tatcactgct atcgtaagaa
     2461 aagaaaagca aatagaaatc gcaaaaaaat taggtgcaaa acaagtaata ataattgaag
     2521 atgataataa accgcttaaa aaaggtattt ttgattattt cttagataca gtggggagta
     2581 atgtaacatt atacggttta aaacgtctaa attatcaagg tgttgctact gtatgtggaa
     2641 atgtagcagg aaattcactt aacgctaata tattaccgtt tattcttaga ggtattaaat
     2701 taattggcat tgattctgta tatgttgatc ataatatccg tgaagatata tggagtaagt
     2761 tagcaaatga atggaatata ggaaattcac ttctatacaa tgaaattgga tttgaagaat
     2821 tctataaaac gatagatcag cttttaaaag gacaacattt aggaagaact attttaaaag
     2881 tataagtgtg agtattcatt aatacattta ctattaattc aatcattttg catttctcta
     2941 ctaaccagtg ttataattta tataacgaaa atgattaaca attaatgcac caatccccac
     3001 actattgcag tagtgtgggg attttttcga tgctggctta taaagtcgcc tattaattat
     3061 ttgttgcgtt tactaagcca ataagtataa tacgcaacag cacagccact gatgactggt
     3121 gctatgatgt gaacgaaaat gattagcaaa gtaatgcacc tcctctcttc gtcaattgac
     3181 gcctgagaga taggcgactc tattattata ccatctgtga atatacaagc atatctactt
     3241 ccgataagtc gtcttatgtt atataacttt gttttttata gtatcaagac aagaagaaac
     3301 tcgtttcaaa aaccctcatg atttttagag aaaaactctc ttatttcatc gtctttaata
     3361 aaataatatg ccatctttcc atctttataa aaatcaagta cctcattttt atataaaagc
     3421 cttaaatgat gtgaagttga agcaacactc attttcaata tcaaagaaat atcacaaaca
     3481 cacaactcat cttctttaat caaagataat atgattttca atttcttctc atcacaaatt
     3541 ttttctaaaa tgttaagtaa tttcttagat ttatcatctt ctaaaaaact taaagcattg
     3601 ttaactttat cctcatgtac acagatcaca tcacaagtat tttcataact catataaaat
     3661 cacaccctaa attaaaatat aaatcctaaa attgtttgaa tagtgtcatt ttcaataata
     3721 ataaataaac ctaaagctat ataaataata gccataatcc aacgactaaa tttctcaaca
     3781 atttctccaa ttcctggaat attagctaat ttttgtgcag taaacaccaa gaagaaaatt
     3841 aaaattaaaa agacaaacag agtaagtaat aaattagtaa cacttaatgt cacaaaatac
     3901 ggaacaaata aaccaatatt atcggcgcca caacttgcta tcgtaacaat tgcaaccgta
     3961 ccaactaatt tagacaatcc tttttcattc aattcttttt tagctctctt ttctccttca
     4021 caatcatcat aaatagccac tttaattcca agataaattg gtattaaacc caataaacct
     4081 aatatccact tttctggaac ataatttaat acaaaagcta aaaacaaact aactaatatt
     4141 aaaataatag accctaaata ttgaccaaca taaatatctc tatattcttt tctagtcttt
     4201 gctctagcaa aaaatattaa taaaattact aataaatcaa ctgctgtagc aatataaaga
     4261 acagcagcag caacaaccgt ttgaatcata atacacctca ttcaaaaata tttttgaatg
     4321 tattataact tatattttta aataatcata ggtgctttat tccaattgct ttattgacgt
     4381 tgagcctcgg aacccttaac aatcccaaaa cttgtcgaat ggtcggctta atagctcacg
     4441 ctatgccgac attcgtctgc aagtttagtt aagggttctt ctcaacatca ataaattttt
     4501 tcggcataaa tgcgtgtcta tgcgtagaca tggagacact tcagaattat ggagtgatta
     4561 catgagttta actatttttc tttattcagt tttaggaagt ttattagcaa attatatcta
     4621 cgaaaaatct tcaaaataaa taactttttc ggttctactt tgtacacttt atctacctgg
     4681 tagacctaat tgttattgct ttacaggtct aaaattcgtc gttataatct agttaaatcc
     4741 cgtaagggcg cacttatacg tcggataaat cgacatgtgc aaaaacgtaa tcgaccttcg
     4801 tcgatttttt tatttcaaaa ttaaagaaac ggcttgagat attccgagca gttataaaaa
     4861 ttaatttgcg ttgaatattg ctactacatt cttgaaaaag tgatgtagat ttatagccca
     4921 taactttttt gaattacatc gacgatacgg cgtcgaaaga aaaataaata gaccgtatga
     4981 aagaaaaagc tacctcactt tatctggggt agcttttatt ttttatgccc ttattttgaa
     5041 ttttaagagg gcattttaaa gatttagggg tgattcatat agttttatat ctaaaactta
     5101 tatatgcttt taaaacgcaa atatgagcca aataaatata tgctattttc aaaaacaaaa
     5161 atttgagcaa aaacaataac tatttttaga cactgcccat ttacatgcaa attaaaaatt
     5221 agcataaaat tctttaaaac ttccccacgt ttttctaaat tcctgtaggg cgtttttatc
     5281 aaaaatcaag acaagaagaa actcgttcac actcgtttca aaaaaacaag aaatctaatc
     5341 tatacaccta ccgttttcca accccctcaa agcatacaaa ttaaaaacaa aattattcat
     5401 aatcttactt tcttttaaca aaaacataaa aaccataaat agacaaatta actatcaaag
     5461 gcaataaaca gtataaaaaa accacgaata aatcttcatc aacatctaaa ataaaaaaga
     5521 ataaaacaat taacaaaata ctaattaaca aacctaaaag tggcaaactt tttatatatt
     5581 tttctaaatt ttcattcata aaatagactc ctttcaaaaa tatgtttata aatatattaa
     5641 aataaatatt gattttataa agcgcttact tatacaatta aaatatctta ttatcttgga
     5701 ggtatcaaat gaaaattata aaattatttt caataatttc cttgttccta ctggtattta
     5761 gcgcgaattt ttctaatgct tacgcaaatg aagaacaaaa aagttcttta ctagaaaatc
     5821 aaaaagaaaa attgaaaagc aaaaacatcg aagtatcaca aatgaccaag aaagaaataa
     5881 gttttctaga acaattgcaa aataaaaatg aagatatgca aaaattcgaa aagcaaatga
     5941 aaaaagaagg atttaaaaaa ataaagctag aagacaaaaa ttctaaatta aaatatctag
     6001 ctacagaagc aaatgataaa aatgcatatg gctacatagc taataacgct tataaaaatg
     6061 aaaaaacaaa agaaattgta gtttctgaaa caatatatga taactacaac aaaaaaatca
     6121 caaaattcat tgccgaaaaa agaagtatga ctgaaaacaa agagggaaaa atacttatta
     6181 acaaaaatta cgttgaatat aaggctgaaa aagttgataa aaacagaccg gtaacatatg
     6241 caggattcaa atggaacggc aaagcattcg cttgtagtat ggctggactt tatgcatgtg
     6301 ctcactactg tggagtatgg gcaatcgtaa atccaatagc cggcggtgta tgtgaagcag
     6361 tttgcggaac tgcatttgca gctggatgct ctgtaggttg attttattcc aattgtttta
     6421 ttgacgttga gcctcggaac ccttaacaat cccaaaactt gtcgaatggt cggcttaata
     6481 gctcacgcta tgccgacatt cgtctgcaag tttagttaag ggttcttctc aacatcaata
     6541 aattttttcg gcataaatac gatgttcctt atttatcctc ataaaaaaca atagctgaaa
     6601 tatataacca attactgtgt gtttgaattt ctaatacatg aatatcattc tttaaaaacc
     6661 tattaacttg tttatctaat tgacgatgag taaaagtagc gaaagtttta atcttcatga
     6721 tatctctcct tttataaaaa tagcaatcct tattacaata ttaaacactt ttattttgga
     6781 ttttaagagg cattttgaag acttagaggc aaatcatata ggttttgtac ttaaaaatct
     6841 atacagtctc ttaaaacgca aatatgagcc aaataaatat atgctaattt cacaaacaaa
     6901 aatttcagca aaatcagtga ctaatttttt agatactgcc catttacatg caaatttaaa
     6961 aattagcata aaatacttta aaacttcccc atgtttttct aaattcctgc agggcttttc
     7021 ttatcaaaaa cttagaaatt ttaatttaac tctaaaatct ccgtaagagc ataagggaaa
     7081 tttgctatca ttcaaaaatg atagatctgg caaggccaga cattccaagg gttttaagtt
     7141 attttaaagt gatatcatac aaagctgtag tgtcacactt ttgtgacact acatacagca
     7201 tttgtcacaa aaataagaca gcactttttt acagaaaaac aataaaaaaa gacatcagtt
     7261 atcactaatg tcttaaataa taattttgct tttcgaaatt ccaaattgcg gtaattgaat
     7321 gtgctttttt tcttcgtctg tcgttttatc ttcatcactt gtattaatca aattaccatc
     7381 ttcggcatcg tctaagttta aaattttatg cttttgttta agcaatccac cataacttaa
     7441 catacgtttt cgatacaaac ctttttctaa atcacttaca atttcttgat ttttttcgtc
     7501 gtcatcagtt aaaaagtcag atgatttaac ggaatatttt gatgtctctt cgagtgttat
     7561 aaaatcattt gaattattga ttaaagatat tatattgttt catgttttta cggcgcatat
     7621 aaagggcatg tgtttcttca agttcagtta gaattgtttg cattaaataa gcttgcattg
     7681 acttgattgt ttccttttgg cctttaactc ccctatcatg tatagctttt ttaagtcttt
     7741 ttaaagaagt taatatttca tcctcaatgt cctctaacat gtaatgcata tcttcatgcc
     7801 ttgaattaaa tgattttttt gctttaagaa taacagcctt tatgatttct aaatctctat
     7861 aatcatattt taataaataa ttagttagtt gttctgggaa ttcttgcaat agcatttctt
     7921 tatcatcaaa attatgtgta aattgatttg aatgatttga atgattacta atttcatttt
     7981 tgtactttat atcattcaag tcattcatat cattattctc agtatcatta aagtcaatgt
     8041 cattaaagtc agtatcatta gtgttcgatt ttcgaacttc tggacgttcg attttcgaac
     8101 ttctggacgt tcgattttcg aacttctgga cgttcgattt tcgaacttcc ttctcgccgt
     8161 aaggttcaac atcgttttct tctttttcta ttttgtaaat atcacgttct gtgacaatag
     8221 gtttcattaa atatagaata tttggtttat ttaaaccacg tcgtttttga atgagtagac
     8281 catcgttttc taaccctttt tttatttttg ttaacttttc ttttttacaa tttaagattt
     8341 ccattagctt ttcatttgaa taaacaaagt aaatgttacc atcttcatca acccagctat
     8401 ttttaataga taaatttaag cgatcacgta agatagcata ggctattttc atatcattag
     8461 ttagattttt ataattttca ctggtaaaga atactttagg gatttgataa aatctttctt
     8521 tgtaattttc ttgcactgta aaatattgat tttgcatgac aaaaaacctc tttccatatt
     8581 ttagtttggt aagaggtaat aaaacgtttt tatttagctt tacatttatt taatgattta
     8641 ggtgcttgca ctaagatatt attcattata taatacaagt atcgaaaatt atataaataa
     8701 acagtttcta aacgttttat taccaaaccc cgactattac cagtagttgg ggtttttgtt
     8761 atattaagtt tattatagca ctttaaataa acttataaaa gggaaaatat agctttatca
     8821 agggtttata aacccttgat aaatatcata aacctttcat aaacccttga taaaaaggtt
     8881 gatggatgtt tatttttatt tattaatcac tttattatta agcattataa gcttataata
     8941 ttttatcaag ggtttataaa cattttataa agggttgatg atatgaaaag tgtgaaaaag
     9001 ttgagtgaag aattaggggt tagtaagcaa acgattttta ataatattaa gcgattgaat
     9061 atagagacaa taaaacagga aaacacttcg tttattaaag aagataccga tatagaaaaa
     9121 atcattcaaa gagtaaatga aaataaaaag aaatatggtt ttgaaagtac tactgaagac
     9181 aaacaaaaaa aagggtcgga taatattaat tttgaatcga aaagcgatgc tcaaatagta
     9241 gaaatcctta aaaatcagat tgatacttta aataatcaaa ttgaaaaaca agaaagtcgt
     9301 catgaaacaa caattgaatt ttacagaaaa gagttgcaag aaagatcaaa attgcttgaa
     9361 aatcagcaag ttctggcatt agagagtaat aaaaagattc aaaaattaga aaatcaatta
     9421 gaaaaagaaa aacaccttaa ttatcccttt gatacatcta ctaacactag acaaaacgtt
     9481 gatgcacaag aaaaaacata tactactaat cctgtaaata ttaacagaga tcaggaagaa
     9541 actaaagaaa cagagattca atataaggat atatctggaa gtcaaagcga cgaaagtaca
     9601 caaggtgaag aagcaaaaaa agaagacgtt ccagctaatc taaatgacaa tgatagtgat
     9661 atagaagaaa agagtgaaga aacggaacct aaaaaggggt tttggagtcg tttgtttggt
     9721 agctaaaata attgattagg cactaaaagt tataaaattt tagtgtcttt tatatagtga
     9781 gaacttctgg taatcatcga ttatgtaaac taaagtattt caaataatta actgtattaa
     9841 tactctcatc atgattgcgt tataaacgtt cattagtgaa acaggtatta tattaaaaaa
     9901 gcgtacgaat ttgtacgctt ttttcttgtg ttattattaa aatctcataa caggaaaata
     9961 ttatgagatt ttagacgctg tcagaacgtc tcttttacat cgagtgacat tgaatcaaag
    10021 tctttgtatg ttaatgatat ataaaattat ttagtagatt cattatagta agctagatta
    10081 gttgctctac ttgtttttat tctcttttgc ctctaattta ttgagataac gataaatagt
    10141 tgtacgtgaa acttgccact gctctgcaat tgttttaatt ggcgtaccat tatcataaag
    10201 tgtcttaagt aagtttaaat cttgtttatt taatttttca ggtctacctc catatcggcc
    10261 tctagcacgt gctgcaattc gaccggctga tgatcgctct aaaattaaat tacgttcaaa
    10321 ctctgcaaat gctgcgaata aatgaaataa taattgtcct gtagaacttg atttatccat
    10381 cgttatattt tcttctaaac tatgaaaact cacacctcgt tcgttaagtt cattaactaa
    10441 cgtgattaaa tcctccatgt tccgtcctag tctatctaat ctccaaacaa caatcgtatc
    10501 tcctgacctc gcaaattcaa ttgctttatc taaaccaggt cgcttacttt ttgaaccact
    10561 catatggtca ttaaatatct tttcacaacc atatgtattt aatcgatcct cctgtaaatt
    10621 caaattttgt aatccagttg aaactcttgc ataacctatt ttcaaaatac agacctccta
    10681 tatacacctt tagtgtacca taacttgatt acgaggtctt ttgtgaacat agattttttg
    10741 ttgggttttt gaacacataa ttctatgtaa tacttactta tttagacaaa aaacaagagt
    10801 gtacagaaaa tggttatttt ctggacactc tcatctttaa taatatattt taaatgtcat
    10861 taacaaaact aatttaataa gagtcaagta tggtttacat atgtattatg tttgaaacat
    10921 acctctaatt actttttact aatatcattt aaaatgtctc gcaattcttc aatttctttg
    10981 ttatttaatt cttcattttt cgcaaaattc agcactaaac ttttcatatc ccctccatac
    11041 agtttattaa gaaaggtttt agcagttttc attttaatat cgtcttcttt aatatttgat
    11101 gagtaaaaat aaatattttc tgatttgtat cgttttataa tctctttttt atatagtctt
    11161 gtgattaatg ttctaatcgt tttatcacta acttctttat atttttgaat ttcaactaca
    11221 atttcattag ctgatactga ttttttatcc catattatat tcataacatc ccattcagcc
    11281 atagatattt caacttgctt attggtcatt taaaacaccc attcctttta atattttttc
    11341 acttattaat tcagcatttt tcccagacgg gtttccatct gataaatgtg tagcaaaata
    11401 atacttatca tgatttgtaa ttacgtaacc tacaaaccac ccattattat acttcccgtt
    11461 tactatacct gtacctgttt tcccatacag ttcatacttt tcatttttct taatcaataa
    11521 tgaagaagat aattgattct ttgctttttt actaaaatgg ttattttgtt ccatcatatt
    11581 tttaaaaact attacttgtt caagattaga tattttcaaa ctatcttcca tccaatagct
    11641 tttataactt cccaaatttt cattaccata atttaattgc ttgagttgag ccgcagtata
    11701 gttctttggt atttgattac taatacgttc gaagtaccaa ttaactgaat tttgcattgc
    11761 tgtatttaaa tcttgttcct tattccaaga ctcgaaaaga taatgtttat gattccatga
    11821 catacgtgaa tttttatcac ttataatatg acggtcaagt ccaaacagtg ctaaataaat
    11881 tttgtaagtt gaatcaggag aatatctttt tctactttct ttttcattat aaatatagta
    11941 tttgtctttc ttcatgctgt acataacgaa agatccacta tttgaaccaa aatttttact
    12001 ttcatctaaa atttggtaat cgctttgtaa gggcttttta taattataat cagttaagga
    12061 ttgccccatt aaaaactggc tttgaattat catgataaaa aaagtaaaaa tacaaataaa
    12121 aattagaatt aattttgact gctttttcag atcggcttct tttatattaa ttaatctact
    12181 tttgagtaat gattttttac cattaaatga atggcttaat atatttttgt tggaaaatgg
    12241 ggtttttaat acggagtcca taatagattc cgcataagtt ttaaattcat ttttgttaat
    12301 attattaagg acaaatctat cggcttctac ttcattgtca tgaattatct ttctttttac
    12361 aatatgtact aatggattgt aactcataat aatagagaag atattaaaga taattaaatg
    12421 taaagtatct ctatttttag catgagcata ttcatgcagt attatatatt ttagtctttt
    12481 atcaattaca cttttaaaat atgaactagg aatcaaaata atatatttcc cataccaaaa
    12541 agttattgga gattgaatag cctctgcttt tcgaatcaca atattttttt tatattgatg
    12601 gttgaaaagt atcgtatcta ttttattttt ttcattttcg tttagataaa gtgactgttt
    12661 ctttaaatat ttaagatata ataaggcttt caaaaattta aaacttaaaa taataactaa
    12721 aactatccaa ataactgtgc agatattatc aattgaatcc caattaaact tatggatatc
    12781 tgttgtgaac tcttgaatag gtttggtggt atttatgtta tggttcaaat cgtgtgactt
    12841 actttcaact gtgggcgctt gattattcac attattaaat tttataaaag agaatttaat
    12901 tgggataaaa ggaattaatc cagcaagcat agttagatac caaactttat aatttaaact
    12961 ataattaaaa tagcgtttta atatgtacct aaaaaatact aacaatagaa atataaaaca
    13021 aaagcttact acgctcatta ttagtaattt agccattttg aaaccttctt tcaaatattt
    13081 ataataaaca attgacatca atattacaat tgtaatatta ttgatttata aaaattacaa
    13141 ctgtaatatc ggagggttta ttt
//
