LOCUS       CP030519               13119 bp    DNA     circular BCT 10-OCT-2019
DEFINITION  Staphylococcus aureus strain ER04332.3 plasmid unnamed1, complete
            sequence.
ACCESSION   CP030519
VERSION     CP030519.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN09484062
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13119)
  AUTHORS   Sullivan,M.J., Altman,D.R., Chacko,K., Cliferri,B., Webster,E.,
            Deikus,G., Lewis,M., Khan,Z., Beckford,C., Rendo,A., Samaroo,F.,
            Sebra,R., Karam-Howlin,R., Southwick,K., Adams,E., Ying,L.,
            Kornblum,J., Factor,S., Danesh Yazdi,M., Dingle,T., Hamula,C.,
            Bashir,A., Schadt,E., Kasarskis,A., Patel,G., Wallach,F., Gibbs,K.
            and van Bakel,H.
  TITLE     Whole genome sequencing to identify and define MRSA outbreaks
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13119)
  AUTHORS   Sullivan,M.J., Altman,D.R., Chacko,K., Cliferri,B., Webster,E.,
            Deikus,G., Lewis,M., Khan,Z., Beckford,C., Rendo,A., Samaroo,F.,
            Sebra,R., Karam-Howlin,R., Southwick,K., Adams,E., Ying,L.,
            Kornblum,J., Factor,S., Danesh Yazdi,M., Dingle,T., Hamula,C.,
            Bashir,A., Schadt,E., Kasarskis,A., Patel,G., Wallach,F., Gibbs,K.
            and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2018) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 318.878374257x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2018 17:50:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,957
            CDS (total)                       :: 2,875
            Genes (coding)                    :: 2,771
            CDS (coding)                      :: 2,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 54 of 104
            Pseudo Genes (incomplete)         :: 34 of 104
            Pseudo Genes (internal stop)      :: 29 of 104
            Pseudo Genes (multiple problems)  :: 13 of 104
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13119
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ER04332.3"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2015"
                     /collected_by="Mount Sinai Hospital"
     gene            join(12908..13119,1..772)
                     /locus_tag="DQV81_14715"
     CDS             join(12908..13119,1..772)
                     /locus_tag="DQV81_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="QFL28045.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication protein"
     gene            765..1081
                     /locus_tag="DQV81_14720"
                     /pseudo=""
     CDS             765..1081
                     /locus_tag="DQV81_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /name="replication protein"
     gene            complement(1945..2637)
                     /locus_tag="DQV81_14725"
     CDS             complement(1945..2637)
                     /locus_tag="DQV81_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QFL28046.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="hypothetical protein"
     gene            2747..2941
                     /locus_tag="DQV81_14730"
     CDS             2747..2941
                     /locus_tag="DQV81_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QFL28047.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            4057..4674
                     /locus_tag="DQV81_14735"
     CDS             4057..4674
                     /locus_tag="DQV81_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium transporter"
                     /protein_id="QFL28048.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQYL
                     GSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNEKG
                     LSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQKLAN
                     IPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
                     /name="cadmium transporter"
     gene            4693..5040
                     /locus_tag="DQV81_14740"
     CDS             4693..5040
                     /locus_tag="DQV81_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="QFL28049.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="transcriptional regulator"
     gene            5197..5289
                     /locus_tag="DQV81_14745"
     CDS             5197..5289
                     /locus_tag="DQV81_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="QFL28050.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5461..6432)
                     /locus_tag="DQV81_14750"
     CDS             complement(5461..6432)
                     /locus_tag="DQV81_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="QFL28051.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="NADPH:quinone reductase"
     gene            6556..6975
                     /locus_tag="DQV81_14755"
     CDS             6556..6975
                     /locus_tag="DQV81_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="QFL28052.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            complement(7197..7292)
                     /locus_tag="DQV81_14760"
                     /pseudo=""
     CDS             complement(7197..7292)
                     /locus_tag="DQV81_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560862.1"
                     /note="incomplete; partial on complete genome; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /name="O-antigen ligase domain-containing protein"
     gene            complement(7501..8346)
                     /locus_tag="DQV81_14765"
     CDS             complement(7501..8346)
                     /locus_tag="DQV81_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="QFL28053.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALNT
                     KSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEKYV
                     GKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIELNYY
                     SPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPKDYKV
                     ADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKSVMKEF
                     "
                     /name="penicillin-hydrolyzing class A beta-lactamase BlaZ"
     gene            8453..10210
                     /locus_tag="DQV81_14770"
     CDS             8453..10210
                     /locus_tag="DQV81_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="QFL28054.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTMLA
                     GLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSIDN
                     ICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIVIRK
                     AEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFNIFSI
                     IMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSNKNILS
                     HSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSLTDYNYK
                     KPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYKIYLALFG
                     LDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIPKNYTAAQL
                     KQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQLSSSLLIKK
                     NEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGKNAELISEKIL
                     KGMGVLNDQ"
                     /name="beta-lactam sensor/signal transducer BlaR1"
     gene            10200..10580
                     /gene="blaI"
                     /locus_tag="DQV81_14775"
     ARG             10201..10577
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            10844..11422
                     /locus_tag="DQV81_14780"
     CDS             10844..11422
                     /locus_tag="DQV81_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="QFL28056.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="HTH domain-containing protein"
     gene            complement(11739..12482)
                     /locus_tag="DQV81_14785"
     CDS             complement(11739..12482)
                     /locus_tag="DQV81_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="QFL28057.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     ARG             8454..10207
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     ARG             complement(7505..8346)
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             4058..4671
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(7502..8346)
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          1138..1285
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.980000000000001e-72"
     Typing          2..772
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.482"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          904..1073
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.605"
                     /coverage="91.0"
                     /evalue="1.04e-65"
     Typing          complement(11740..12482)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatggaaatc ttaaattgta aaaaagaaaa gttaacaaaa ataaaaaaag ggttagaaaa
       61 cgatggtcta ctcattcaaa aacgacgtgg tttaaataaa ccaaatattc tatatttaat
      121 gaaacctatt gtcacagaac gtgatattta caaaatagaa aaagaagaaa acgatgttga
      181 accttacggc gagaaggaag ttcgaaaatc gaacgtccag aagttcgaaa atcgaacgtc
      241 cagaagttcg aaaatcgaac gtccagaagt tcgaaaatcg aacactaatg atactgactt
      301 taatgacatt gactttaatg atactgagaa taatgatatg aatgacttga atgatataaa
      361 gtacaaaaat gaaattagta atcattcaaa tcattcaaat caatttacac ataattttga
      421 tgataaagaa atgctattgc aagaattccc agaacaacta actaattatt tattaaaata
      481 tgattataga gatttagaaa tcataaaggc tgttattctt aaagcaaaaa aatcatttaa
      541 ttcaaggcat gaagatatgc attacatgtt agaggacatt gaggatgaaa tattaacttc
      601 tttaaaaaga cttaaaaaag ctatacatga taggggagtt aaaggccaaa aggaaacaat
      661 caagtcaatg caagcttatt taatgcaaac aattctaact gaacttgaag aaacacatgc
      721 cctttatatg cgccgtaaaa acatgaaaca atataatatc tttaatcaat aattcaaatg
      781 attttataac actcgaagag acatcaaaat attccgttaa atcatctgac tttttaactg
      841 atgacgacga aaaaaatcaa gaaattgtaa gtgatttaga aaaaggtttg tatcgaaaac
      901 gtatgttaag ttatggtgga ttgcttaaac aaaagcataa aattttaaac ttagacgatg
      961 ccgaagatgg taatttgatt aatacaagtg atgaagataa aacgacagac gaagaaaaaa
     1021 agcacattca attaccgcaa tttggaattt cgaaaagcaa aattattatt taagacatta
     1081 gtgataactg atgtcttttt ttattgtttt tctgtaaaaa agtgctgtct tatttttgtg
     1141 acaaatgctg tatgtagtgt cacaaaagtg tgacactaca gctttgtatg atatcacttt
     1201 aaaataactt aaaacccttg gaatgtctgg ccttgccaga tctatcattt ttgaatgata
     1261 gcaaatttcc cttatgctct tacggagatt ttagagttaa attaaaattt ctaagttttt
     1321 gataagaaaa gccctgcagg aatttagaaa aacatgggga agttttaaag tattttatgc
     1381 taatttttaa atttgcatgt aaatgggcag tatctaaaaa attagtcact gattttgctg
     1441 aaatttttgt ttgtgaaatt agcatatatt tatttggctc atatttgcgt tttaagagac
     1501 tgtatagatt tttaagtaca aaacctatat gatttgcctc taagtcttca aaatgcctct
     1561 taaaatccaa aataaaagtg tttaatattg taataaggat tgctattttt ataaaaggag
     1621 agatatcatg aagattaaaa ctttcgctac ttttactcat cgtcaattag ataaacaagt
     1681 taataggttt ttaaagaatg atattcatgt attagaaatt caaacacaca gtaattggtt
     1741 atatatttca gctattgttt tttatgagga taaataagga acatcgtatt tatgccgaaa
     1801 aaatttattg atgttgagaa gaacccttaa ctaaacttgc agacgaatgt cggcatagcg
     1861 tgagctatta agccgaccat tcgacaagtt ttgggattgt taagggttcc gaggctcaac
     1921 gtcaataaaa caattggaat aaaatcaacc tacagagcat ccagctgcaa atgcagttcc
     1981 gcaaactgct tcacatacac cgccggctat tggatttacg attgcccata ctccacagta
     2041 gtgagcacat gcataaagtc cagccatact acaagcgaat gctttgccgt tccatttgaa
     2101 tcctgcatat gttaccggtc tgtttttatc aactttttca gccttatatt caacgtaatt
     2161 tttgttaata agtatttttc cctctttgtt ttcagtcata cttctttttt cggcaatgaa
     2221 ttttgtgatt tttttgttgt agttatcata tattgtttca gaaactacaa tttcttttgt
     2281 tttttcattt ttataagcgt tattagctat gtagccatat gcatttttat catttgcttc
     2341 tgtagctaga tattttaatt tagaattttt gtcttctagc tttatttttt taaatccttc
     2401 ttttttcatt tgcttttcga atttttgcat atcttcattt ttattttgca attgttctag
     2461 aaaacttatt tctttcttgg tcatttgtga tacttcgatg tttttgcttt tcaatttttc
     2521 tttttgattt tctagtaaag aacttttttg ttcttcattt gcgtaagcat tagaaaaatt
     2581 cgcgctaaat accagtagga acaaggaaat tattgaaaat aattttataa ttttcatttg
     2641 atacctccaa gataataaga tattttaatt gtataagtaa gcgctttata aaatcaatat
     2701 ttattttaat atatttataa acatattttt gaaaggagtc tattttatga atgaaaattt
     2761 agaaaaatat ataaaaagtt tgccactttt aggtttgtta attagtattt tgttaattgt
     2821 tttattcttt tttattttag atgttgatga agatttattc gtggtttttt tatactgttt
     2881 attgcctttg atagttaatt tgtctattta tggtttttat gtttttgtta aaagaaagta
     2941 agattatgaa taattttgtt tttaatttgt atgctttgag ggggttggaa aacggtaggt
     3001 gtatagatta gatttcttgt ttttttgaaa cgagtgtgaa cgagtttctt cttgtcttga
     3061 tttttgataa aaacgcccta caggaattta gaaaaacgtg gggaagtttt aaagaatttt
     3121 atgctaattt ttaatttgca tgtaaatggg cagtgtctaa aaatagttat tgtttttgct
     3181 caaatttttg tttttgaaaa tagcatatat ttatttggct catatttgcg ttttaaaagc
     3241 atatataagt tttagatata aaactatatg aatcacccct aaatctttaa aatgccctct
     3301 taaaattcaa aataagggca taaaaaataa aagctacccc agataaagtg aggtagcttt
     3361 ttctttcata cggtctattt atttttcttt cgacgccgta tcgtcgatgt aattcaaaaa
     3421 agttatgggc tataaatcta catcactttt tcaagaatgt agtagcaata ttcaacgcaa
     3481 attaattttt ataactgctc ggaatatctc aagccgtttc tttaattttg aaataaaaaa
     3541 atcgacgaag gtcgattacg tttttgcaca tgtcgattta tccgacgtat aagtgcgccc
     3601 ttacgggatt taactagatt ataacgacga attttagacc tgtaaagcaa taacaattag
     3661 gtctaccagg tagataaagt gtacaaagta gaaccgaaaa agttatttat tttgaagatt
     3721 tttcgtagat ataatttgct aataaacttc ctaaaactga ataaagaaaa atagttaaac
     3781 tcatgtaatc actccataat tctgaagtgt ctccatgtct acgcatagac acgcatttat
     3841 gccgaaaaaa tttattgatg ttgagaagaa cccttaacta aacttgcaga cgaatgtcgg
     3901 catagcgtga gctattaagc cgaccattcg acaagttttg ggattgttaa gggttccgag
     3961 gctcaacgtc aataaagcaa ttggaataaa gcacctatga ttatttaaaa atataagtta
     4021 taatacattc aaaaatattt ttgaatgagg tgtattatga ttcaaacggt tgttgctgct
     4081 gctgttcttt atattgctac agcagttgat ttattagtaa ttttattaat attttttgct
     4141 agagcaaaga ctagaaaaga atatagagat atttatgttg gtcaatattt agggtctatt
     4201 attttaatat tagttagttt gtttttagct tttgtattaa attatgttcc agaaaagtgg
     4261 atattaggtt tattgggttt aataccaatt tatcttggaa ttaaagtggc tatttatgat
     4321 gattgtgaag gagaaaagag agctaaaaaa gaattgaatg aaaaaggatt gtctaaatta
     4381 gttggtacgg ttgcaattgt tacgatagca agttgtggcg ccgataatat tggtttattt
     4441 gttccgtatt ttgtgacatt aagtgttact aatttattac ttactctgtt tgtcttttta
     4501 attttaattt tcttcttggt gtttactgca caaaaattag ctaatattcc aggaattgga
     4561 gaaattgttg agaaatttag tcgttggatt atggctatta tttatatagc tttaggttta
     4621 tttattatta ttgaaaatga cactattcaa acaattttag gatttatatt ttaatttagg
     4681 gtgtgatttt atatgagtta tgaaaatact tgtgatgtga tctgtgtaca tgaggataaa
     4741 gttaacaatg ctttaagttt tttagaagat gataaatcta agaaattact taacatttta
     4801 gaaaaaattt gtgatgagaa gaaattgaaa atcatattat ctttgattaa agaagatgag
     4861 ttgtgtgttt gtgatatttc tttgatattg aaaatgagtg ttgcttcaac ttcacatcat
     4921 ttaaggcttt tatataaaaa tgaggtactt gatttttata aagatggaaa gatggcatat
     4981 tattttatta aagacgatga aataagagag tttttctcta aaaatcatga gggtttttga
     5041 aacgagtttc ttcttgtctt gatactataa aaaacaaagt tatataacat aagacgactt
     5101 atcggaagta gatatgcttg tatattcaca gatggtataa taatagagtc gcctatctct
     5161 caggcgtcaa ttgacgaaga gaggaggtgc attactttgc taatcatttt cgttcacatc
     5221 atagcaccag tcatcagtgg ctgtgctgtt gcgtattata cttattggct tagtaaacgc
     5281 aacaaataat taataggcga ctttataagc cagcatcgaa aaaatcccca cactactgca
     5341 atagtgtggg gattggtgca ttaattgtta atcattttcg ttatataaat tataacactg
     5401 gttagtagag aaatgcaaaa tgattgaatt aatagtaaat gtattaatga atactcacac
     5461 ttatactttt aaaatagttc ttcctaaatg ttgtcctttt aaaagctgat ctatcgtttt
     5521 atagaattct tcaaatccaa tttcattgta tagaagtgaa tttcctatat tccattcatt
     5581 tgctaactta ctccatatat cttcacggat attatgatca acatatacag aatcaatgcc
     5641 aattaattta atacctctaa gaataaacgg taatatatta gcgttaagtg aatttcctgc
     5701 tacatttcca catacagtag caacaccttg ataatttaga cgttttaaac cgtataatgt
     5761 tacattactc cccactgtat ctaagaaata atcaaaaata ccttttttaa gcggtttatt
     5821 atcatcttca attattatta cttgttttgc acctaatttt tttgcgattt ctatttgctt
     5881 ttcttttctt acgatagcag tgatattttc atatccaatt ttatgtaaaa tttgtatagc
     5941 aacactgccc acaccacctg tagccccaga cactaaaatt tcaggttgtg atttgatatt
     6001 cattccatat ttttctagtt caaaaattga taatgctgcc gtaaaaccgg ctgtgccata
     6061 taccatagac tctttaatag acatttcttg gggaatagga acaatccact ctccaggcac
     6121 ttgtgcatat tctgaaaatc cacctgtatg tgtcacgcca acatcataac cagtaactaa
     6181 tacattatcg ccttctttaa acttatcatt cgaacttgat tcaacaatac ctgcaaaatc
     6241 tataccagga atcataggat aatctctaat tacaccgcct ttatattgta aagctagcat
     6301 atccttataa tttattgaag agtataccac ttttactaat acatcaccct tcgataaatg
     6361 agattcatca actttttcta ctttataact aacatttgat gcactttttc tcactactac
     6421 cgcattgaac atttattagc cttctttcac aattgttttg tagaagtatg tttttgttgc
     6481 aaaactattt tcctacaaaa cttatcatga tatgataagc gagtcaatac taatgattta
     6541 agagaggttt tagttgtgga attagataat tgtataaatt atttgttgag tacttctcag
     6601 aatagagttt ttaaacattt taaaagttta ttaagtccct ataatattac tccagcagaa
     6661 tatggtgttt tgaattgctt gattaatagt aatttgaata cacctaaagc gattggtaat
     6721 actttaaatt tagaaccttc aacaatatct ggaatattag ataagatgtc caaaaaggat
     6781 ctaataacac gtcaaactga taatgaaaat agaagaactg tgctaataaa cagtacaaat
     6841 aaagcaaaag acttatggcc aacattagaa aagaaagcct atcaacttaa taatcattac
     6901 ttcgctaatt taactaatga agaaactgta gtgtttaaaa agatattact aaagataaat
     6961 aaaactacgt tttaattttt acgttataaa aaagttgaag tgcgaattat aatcgagtaa
     7021 atataaatga tagcttagct taattatata ttaaagccaa ctatactttt attcccgaaa
     7081 aacctactat ccaaatttct aagatacttt agcctctata ataaacatat tgtaatataa
     7141 tttatgtatt atttaggttt accctaatcg aacaataaaa aatacttgta aaatgctatt
     7201 gtcactttgc ttgatatatg agaattattt aaccttataa atgagaaaaa agcaacgcac
     7261 tttaaataag atacgttgct ttttcgattg atgaacacct ataattaaac tattcatcta
     7321 ttatttatga ttttttgtat atacaatatt tctagtttgt taaagagaat taagaaaata
     7381 aatctcgaaa ataataaagg gaaaatcagt ttttgatatc aaaattatac atgtcaacga
     7441 taatacaaaa tataatacaa actataagat gttatcagta tttattatgc atttagaata
     7501 ttaaaattcc ttcattacac tcttggcggt ttcacttatc aacttatcat ttggcttatc
     7561 acttttattg tctttattcg taaaaatgac taaaacaata ggttcagatt ggcccttagg
     7621 ataaacaaaa gcaacatcat ttctagaagc atatgttatt gcttgaccac ttttatcagc
     7681 aaccttatag tcttttggaa caccatcttt aattaaagtg tctccgtttt tattattaaa
     7741 cattaaatca agtaagaaat ttttattttt tttgcttaat tttccatttg cgataagttt
     7801 atttaaagtc ttgccgaaag cagcaggcgt tgaagtatct tttttgctct ttggtgaata
     7861 gtaatttaat tctatctcat atctaactgg atttgttact ttatctccta gttcttttag
     7921 acgttgttta acttttttga ttccaccgat ttcttttata attttattgt ttgctgtatt
     7981 atcactatat gtcattgaag cctcaataag ttcttttaaa gtgatatctt ttcctacata
     8041 tttttctaaa ataggagaat aagcaactat atcatctttg ttaatatgta cttttttatt
     8101 taacttatta taaggtactt gttctaacaa aatagcacta tttatcgctt ttgaagtcga
     8161 agcataggca aatctcttat ctgaattaaa ttttacttcc ttaccacttt tagtatttaa
     8221 agcataaaca ccaatatgag cattatattt tttttctaaa tcatttaact ctttggcatg
     8281 tgaactgttt gaattacatg cacttaaaac taaagcaatt acaattaaaa atattaactt
     8341 tttcaaaata aaccctccga tattacagtt gtaattttta taaatcaata atattacaat
     8401 tgtaatattg atgtcaattg tttattataa atatttgaaa gaaggtttca aaatggctaa
     8461 attactaata atgagcgtag taagcttttg ttttatattt ctattgttag tattttttag
     8521 gtacatatta aaacgctatt ttaattatag tttaaattat aaagtttggt atctaactat
     8581 gcttgctgga ttaattcctt ttatcccaat taaattctct tttataaaat ttaataatgt
     8641 gaataatcaa gcgcccacag ttgaaagtaa gtcacacgat ttgaaccata acataaatac
     8701 caccaaacct attcaagagt tcacaacaga tatccataag tttaattggg attcaattga
     8761 taatatctgc acagttattt ggatagtttt agttattatt ttaagtttta aatttttgaa
     8821 agccttatta tatcttaaat atttaaagaa acagtcactt tatctaaacg aaaatgaaaa
     8881 aaataaaata gatacgatac ttttcaacca tcaatataaa aaaaatattg tgattcgaaa
     8941 agcagaggct attcaatctc caataacttt ttggtatggg aaatatatta ttttgattcc
     9001 tagttcatat tttaaaagtg taattgataa aagactaaaa tatataatac tgcatgaata
     9061 tgctcatgct aaaaatagag atactttaca tttaattatc tttaatatct tctctattat
     9121 tatgagttac aatccattag tacatattgt aaaaagaaag ataattcatg acaatgaagt
     9181 agaagccgat agatttgtcc ttaataatat taacaaaaat gaatttaaaa cttatgcgga
     9241 atctattatg gactccgtat taaaaacccc attttccaac aaaaatatat taagccattc
     9301 atttaatggt aaaaaatcat tactcaaaag tagattaatt aatataaaag aagccgatct
     9361 gaaaaagcag tcaaaattaa ttctaatttt tatttgtatt tttacttttt ttatcatgat
     9421 aattcaaagc cagtttttaa tggggcaatc cttaactgat tataattata aaaagccctt
     9481 acaaagcgat taccaaattt tagatgaaag taaaaatttt ggttcaaata gtggatcttt
     9541 cgttatgtac agcatgaaga aagacaaata ctatatttat aatgaaaaag aaagtagaaa
     9601 aagatattct cctgattcaa cttacaaaat ttatttagca ctgtttggac ttgaccgtca
     9661 tattataagt gataaaaatt cacgtatgtc atggaatcat aaacattatc ttttcgagtc
     9721 ttggaataag gaacaagatt taaatacagc aatgcaaaat tcagttaatt ggtacttcga
     9781 acgtattagt aatcaaatac caaagaacta tactgcggct caactcaagc aattaaatta
     9841 tggtaatgaa aatttgggaa gttataaaag ctattggatg gaagatagtt tgaaaatatc
     9901 taatcttgaa caagtaatag tttttaaaaa tatgatggaa caaaataacc attttagtaa
     9961 aaaagcaaag aatcaattat cttcttcatt attgattaag aaaaatgaaa agtatgaact
    10021 gtatgggaaa acaggtacag gtatagtaaa cgggaagtat aataatgggt ggtttgtagg
    10081 ttacgtaatt acaaatcatg ataagtatta ttttgctaca catttatcag atggaaaccc
    10141 gtctgggaaa aatgctgaat taataagtga aaaaatatta aaaggaatgg gtgttttaaa
    10201 tgaccaataa gcaagttgaa atatctatgg ctgaatggga tgttatgaat ataatatggg
    10261 ataaaaaatc agtatcagct aatgaaattg tagttgaaat tcaaaaatat aaagaagtta
    10321 gtgataaaac gattagaaca ttaatcacaa gactatataa aaaagagatt ataaaacgat
    10381 acaaatcaga aaatatttat ttttactcat caaatattaa agaagacgat attaaaatga
    10441 aaactgctaa aacctttctt aataaactgt atggagggga tatgaaaagt ttagtgctga
    10501 attttgcgaa aaatgaagaa ttaaataaca aagaaattga agaattgcga gacattttaa
    10561 atgatattag taaaaagtaa ttagaggtat gtttcaaaca taatacatat gtaaaccata
    10621 cttgactctt attaaattag ttttgttaat gacatttaaa atatattatt aaagatgaga
    10681 gtgtccagaa aataaccatt ttctgtacac tcttgttttt tgtctaaata agtaagtatt
    10741 acatagaatt atgtgttcaa aaacccaaca aaaaatctat gttcacaaaa gacctcgtaa
    10801 tcaagttatg gtacactaaa ggtgtatata ggaggtctgt attttgaaaa taggttatgc
    10861 aagagtttca actggattac aaaatttgaa tttacaggag gatcgattaa atacatatgg
    10921 ttgtgaaaag atatttaatg accatatgag tggttcaaaa agtaagcgac ctggtttaga
    10981 taaagcaatt gaatttgcga ggtcaggaga tacgattgtt gtttggagat tagatagact
    11041 aggacggaac atggaggatt taatcacgtt agttaatgaa cttaacgaac gaggtgtgag
    11101 ttttcatagt ttagaagaaa atataacgat ggataaatca agttctacag gacaattatt
    11161 atttcattta ttcgcagcat ttgcagagtt tgaacgtaat ttaattttag agcgatcatc
    11221 agccggtcga attgcagcac gtgctagagg ccgatatgga ggtagacctg aaaaattaaa
    11281 taaacaagat ttaaacttac ttaagacact ttatgataat ggtacgccaa ttaaaacaat
    11341 tgcagagcag tggcaagttt cacgtacaac tatttatcgt tatctcaata aattagaggc
    11401 aaaagagaat aaaaacaagt agagcaacta atctagctta ctataatgaa tctactaaat
    11461 aattttatat atcattaaca tacaaagact ttgattcaat gtcactcgat gtaaaagaga
    11521 cgttctgaca gcgtctaaaa tctcataata ttttcctgtt atgagatttt aatataatac
    11581 ctgtttcact aatgaacgtt tataacgcaa tcatgatgag agtattaata cagttaatta
    11641 tttgaaatac tttagtttac ataatcgatg attaccagaa gttctcacta tataaaagac
    11701 actaaaattt tataactttt agtgcctaat caattatttt agctaccaaa caaacgactc
    11761 caaaacccct ttttaggttc cgtttcttca ctcttttctt ctatatcact atcattgtca
    11821 tttagattag ctggaacgtc ttcttttttt gcttcttcac cttgtgtact ttcgtcgctt
    11881 tgacttccag atatatcctt atattgaatc tctgtttctt tagtttcttc ctgatctctg
    11941 ttaatattta caggattagt agtatatgtt ttttcttgtg catcaacgtt ttgtctagtg
    12001 ttagtagatg tatcaaaggg ataattaagg tgtttttctt tttctaattg attttctaat
    12061 ttttgaatct ttttattact ctctaatgcc agaacttgct gattttcaag caattttgat
    12121 ctttcttgca actcttttct gtaaaattca attgttgttt catgacgact ttcttgtttt
    12181 tcaatttgat tatttaaagt atcaatctga tttttaagga tttctactat ttgagcatcg
    12241 cttttcgatt caaaattaat attatccgac cctttttttt gtttgtcttc agtagtactt
    12301 tcaaaaccat atttcttttt attttcattt actctttgaa tgattttttc tatatcggta
    12361 tcttctttaa taaacgaagt gttttcctgt tttattgtct ctatattcaa tcgcttaata
    12421 ttattaaaaa tcgtttgctt actaacccct aattcttcac tcaacttttt cacacttttc
    12481 atatcatcaa ccctttataa aatgtttata aacccttgat aaaatattat aagcttataa
    12541 tgcttaataa taaagtgatt aataaataaa aataaacatc catcaacctt tttatcaagg
    12601 gtttatgaaa ggtttatgat atttatcaag ggtttataaa cccttgataa agctatattt
    12661 tcccttttat aagtttattt aaagtgctat aataaactta atataacaaa aaccccaact
    12721 actggtaata gtcggggttt ggtaataaaa cgtttagaaa ctgtttattt atataatttt
    12781 cgatacttgt attatataat gaataatatc ttagtgcaag cacctaaatc attaaataaa
    12841 tgtaaagcta aataaaaacg ttttattacc tcttaccaaa ctaaaatatg gaaagaggtt
    12901 ttttgtcatg caaaatcaat attttacagt gcaagaaaat tacaaagaaa gattttatca
    12961 aatccctaaa gtattcttta ccagtgaaaa ttataaaaat ctaactaatg atatgaaaat
    13021 agcctatgct atcttacgtg atcgcttaaa tttatctatt aaaaatagct gggttgatga
    13081 agatggtaac atttactttg tttattcaaa tgaaaagct
//
