LOCUS       CP030517               13163 bp    DNA     circular BCT 10-OCT-2019
DEFINITION  Staphylococcus aureus strain ER01116.3 plasmid unnamed2, complete
            sequence.
ACCESSION   CP030517
VERSION     CP030517.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN09483941
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Sullivan,M.J., Altman,D.R., Chacko,K., Cliferri,B., Webster,E.,
            Deikus,G., Lewis,M., Khan,Z., Beckford,C., Rendo,A., Samaroo,F.,
            Sebra,R., Karam-Howlin,R., Southwick,K., Adams,E., Ying,L.,
            Kornblum,J., Factor,S., Danesh Yazdi,M., Dingle,T., Hamula,C.,
            Bashir,A., Schadt,E., Kasarskis,A., Patel,G., Wallach,F., Gibbs,K.
            and van Bakel,H.
  TITLE     Whole genome sequencing to identify and define MRSA outbreaks
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13163)
  AUTHORS   Sullivan,M.J., Altman,D.R., Chacko,K., Cliferri,B., Webster,E.,
            Deikus,G., Lewis,M., Khan,Z., Beckford,C., Rendo,A., Samaroo,F.,
            Sebra,R., Karam-Howlin,R., Southwick,K., Adams,E., Ying,L.,
            Kornblum,J., Factor,S., Danesh Yazdi,M., Dingle,T., Hamula,C.,
            Bashir,A., Schadt,E., Kasarskis,A., Patel,G., Wallach,F., Gibbs,K.
            and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2018) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58.9894658071x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2018 18:11:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,039
            CDS (total)                       :: 2,957
            Genes (coding)                    :: 2,871
            CDS (coding)                      :: 2,871
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 49 of 86
            Pseudo Genes (incomplete)         :: 34 of 86
            Pseudo Genes (internal stop)      :: 22 of 86
            Pseudo Genes (multiple problems)  :: 18 of 86
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ER01116.3"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2015"
                     /collected_by="Mount Sinai Hospital"
     gene            join(11408..13163,1..2)
                     /locus_tag="DQU60_15125"
     CDS             join(11408..13163,1..2)
                     /locus_tag="DQU60_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="QFL53456.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTMLA
                     GLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSIDN
                     ICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIVIRK
                     AEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFNIFSI
                     IMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSNKNILS
                     HSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSLTDYNYK
                     KPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYKIYLALFG
                     LDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIPKNYTAAQL
                     KQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQLSSSLLIKK
                     NEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGKNAELISEKIL
                     KGMGVLNDQ"
                     /name="beta-lactam sensor/signal transducer BlaR1"
     gene            join(13155..13163,1..372)
                     /gene="blaI"
                     /locus_tag="DQU60_15130"
     ARG             2..369
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="99.19"
                     /coverage="97.62"
     gene            636..1214
                     /locus_tag="DQU60_15135"
     CDS             636..1214
                     /locus_tag="DQU60_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="QFL53445.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="HTH domain-containing protein"
     gene            complement(1575..2318)
                     /locus_tag="DQU60_15140"
     CDS             complement(1575..2318)
                     /locus_tag="DQU60_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="QFL53446.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     gene            2744..3727
                     /locus_tag="DQU60_15145"
     CDS             2744..3727
                     /locus_tag="DQU60_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="QFL53447.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication protein"
     gene            3720..4036
                     /locus_tag="DQU60_15150"
                     /pseudo=""
     CDS             3720..4036
                     /locus_tag="DQU60_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /name="replication protein"
     gene            complement(4900..5592)
                     /locus_tag="DQU60_15155"
     CDS             complement(4900..5592)
                     /locus_tag="DQU60_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QFL53448.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="hypothetical protein"
     gene            5702..5896
                     /locus_tag="DQU60_15160"
     CDS             5702..5896
                     /locus_tag="DQU60_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QFL53449.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            7012..7629
                     /locus_tag="DQU60_15165"
     CDS             7012..7629
                     /locus_tag="DQU60_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium transporter"
                     /protein_id="QFL53450.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQYL
                     GSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNEKG
                     LSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQKLAN
                     IPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
                     /name="cadmium transporter"
     gene            7648..7995
                     /locus_tag="DQU60_15170"
     CDS             7648..7995
                     /locus_tag="DQU60_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="QFL53451.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="transcriptional regulator"
     gene            8152..8244
                     /locus_tag="DQU60_15175"
     CDS             8152..8244
                     /locus_tag="DQU60_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="QFL53452.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(8416..9387)
                     /locus_tag="DQU60_15180"
     CDS             complement(8416..9387)
                     /locus_tag="DQU60_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="QFL53453.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="NADPH:quinone reductase"
     gene            9511..9930
                     /locus_tag="DQU60_15185"
     CDS             9511..9930
                     /locus_tag="DQU60_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="QFL53454.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            complement(10152..10247)
                     /locus_tag="DQU60_15190"
                     /pseudo=""
     CDS             complement(10152..10247)
                     /locus_tag="DQU60_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560862.1"
                     /note="incomplete; partial on complete genome; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /name="O-antigen ligase domain-containing protein"
     gene            complement(10456..11301)
                     /locus_tag="DQU60_15195"
     CDS             complement(10456..11301)
                     /locus_tag="DQU60_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="QFL53455.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALNT
                     KSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEKYV
                     GKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIELNYY
                     SPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPKDYKV
                     ADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKSVMKEF
                     "
                     /name="penicillin-hydrolyzing class A beta-lactamase BlaZ"
     ARG             11409..13162
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     ARG             complement(10460..11301)
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             7013..7626
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(10457..11301)
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          4093..4240
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.980000000000001e-72"
     Typing          complement(1576..2318)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2745..3727
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3859..4028
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.605"
                     /coverage="91.0"
                     /evalue="1.04e-65"
ORIGIN
        1 aagcaagttg aaatatctat ggctgaatgg gatgttatga atataatatg ggataaaaaa
       61 tcagtatcag ctaatgaaat tgtagttgaa attcaaaaat ataaagaagt tagtgataaa
      121 acgattagaa cattaatcac aagactatat aaaaaagaga ttataaaacg atacaaatca
      181 gaaaatattt atttttactc atcaaatatt aaagaagacg atattaaaat gaaaactgct
      241 aaaacctttc ttaataaact gtatggaggg gatatgaaaa gtttagtgct gaattttgcg
      301 aaaaatgaag aattaaataa caaagaaatt gaagaattgc gagacatttt aaatgatatt
      361 agtaaaaagt aattagaggt atgtttcaaa cataatacat atgtaaacca tacttgactc
      421 ttattaaatt agttttgtta atgacattta aaatatatta ttaaagatga gagtgtccag
      481 aaaataacca ttttctgtac actcttgttt tttgtctaaa taagtaagta ttacatagaa
      541 ttatgtgttc aaaaacccaa caaaaaatct atgttcacaa aagacctcgt aatcaagtta
      601 tggtacacta aaggtgtata taggaggtct gtattttgaa aataggttat gcaagagttt
      661 caactggatt acaaaatttg aatttacagg aggatcgatt aaatacatat ggttgtgaaa
      721 agatatttaa tgaccatatg agtggttcaa aaagtaagcg acctggttta gataaagcaa
      781 ttgaatttgc gaggtcagga gatacgattg ttgtttggag attagataga ctaggacgga
      841 acatggagga tttaatcacg ttagttaatg aacttaacga acgaggtgtg agttttcata
      901 gtttagaaga aaatataacg atggataaat caagttctac aggacaatta ttatttcatt
      961 tattcgcagc atttgcagag tttgaacgta atttaatttt agagcgatca tcagccggtc
     1021 gaattgcagc acgtgctaga ggccgatatg gaggtagacc tgaaaaatta aataaacaag
     1081 atttaaactt acttaagaca ctttatgata atggtacgcc aattaaaaca attgcagagc
     1141 agtggcaagt ttcacgtaca actatttatc gttatctcaa taaattagag gcaaaagaga
     1201 ataaaaacaa gtagagcaac taatctagct tactataatg aatctactaa ataattttat
     1261 atatcattaa catacaaaga ctttgattca atgtcactcg atgtaaaaga gacgttctga
     1321 cagcgtctaa aatctcataa tattttcctg ttatgagatt ttaataataa cacaagaaaa
     1381 aagcgtacaa attcgtacgc ttttttaata taatacctgt ttcactaatg aacgtttata
     1441 acgcaatcat gatgagagta ttaatacagt taattatttg aaatacttta gtttacataa
     1501 tcgatgatta ccagaagttc tcactatata aaagacacta aaattttata acttttagtg
     1561 cctaatcaat tattttagct accaaacaaa cgactccaaa accccttttt aggttccgtt
     1621 tcttcactct tttcttctat atcactatca ttgtcattta gattagctgg aacgtcttct
     1681 ttttttgctt cttcaccttg tgtactttcg tcgctttgac ttccagatat atccttatat
     1741 tgaatctctg tttctttagt ttcttcctga tctctgttaa tatttacagg attagtagta
     1801 tatgtttttt cttgtgcatc aacgttttgt ctagtgttag tagatgtatc aaagggataa
     1861 ttaaggtgtt tttctttttc taattgattt tctaattttt gaatcttttt attactctct
     1921 aatgccagaa cttgctgatt ttcaagcaat tttgatcttt cttgcaactc ttttctgtaa
     1981 aattcaattg ttgtttcatg acgactttct tgtttttcaa tttgattatt taaagtatca
     2041 atctgatttt taaggatttc tactatttga gcatcgcttt tcgattcaaa attaatatta
     2101 tccgaccctt ttttttgttt gtcttcagta gtactttcaa aaccatattt ctttttattt
     2161 tcatttactc tttgaatgat tttttctata tcggtatctt ctttaataaa cgaagtgttt
     2221 tcctgtttta ttgtctctat attcaatcgc ttaatattat taaaaatcgt ttgcttacta
     2281 acccctaatt cttcactcaa ctttttcaca cttttcatat catcaaccct ttataaaatg
     2341 tttataaacc cttgataaaa tattataagc ttataatgct taataataaa gtgattaata
     2401 aataaaaata aacatccatc aaccttttta tcaagggttt atgaaaggtt tatgatattt
     2461 atcaagggtt tataaaccct tgataaagct atattttccc ttttataagt ttatttaaag
     2521 tgctataata aacttaatat aacaaaaacc ccaactactg gtaatagtcg gggtttggta
     2581 ataaaacgtt tagaaactgt ttatttatat aattttcgat acttgtatta tataatgaat
     2641 aatatcttag tgcaagcacc taaatcatta aataaatgta aagctaaata aaaacgtttt
     2701 attacctctt accaaactaa aatatggaaa gaggtttttt gtcatgcaaa atcaatattt
     2761 tacagtgcaa gaaaattaca aagaaagatt ttatcaaatc cctaaagtat tctttaccag
     2821 tgaaaattat aaaaatctaa ctaatgatat gaaaatagcc tatgctatct tacgtgatcg
     2881 cttaaattta tctattaaaa atagctgggt tgatgaagat ggtaacattt actttgttta
     2941 ttcaaatgaa aagctaatgg aaatcttaaa ttgtaaaaaa gaaaagttaa caaaaataaa
     3001 aaaagggtta gaaaacgatg gtctactcat tcaaaaacga cgtggtttaa ataaaccaaa
     3061 tattctatat ttaatgaaac ctattgtcac agaacgtgat atttacaaaa tagaaaaaga
     3121 agaaaacgat gttgaacctt acggcgagaa ggaagttcga aaatcgaacg tccagaagtt
     3181 cgaaaatcga acgtccagaa gttcgaaaat cgaacgtcca gaagttcgaa aatcgaacac
     3241 taatgatact gactttaatg acattgactt taatgatact gagaataatg atatgaatga
     3301 cttgaatgat ataaagtaca aaaatgaaat tagtaatcat tcaaatcatt caaatcaatt
     3361 tacacataat tttgatgata aagaaatgct attgcaagaa ttcccagaac aactaactaa
     3421 ttatttatta aaatatgatt atagagattt agaaatcata aaggctgtta ttcttaaagc
     3481 aaaaaaatca tttaattcaa ggcatgaaga tatgcattac atgttagagg acattgagga
     3541 tgaaatatta acttctttaa aaagacttaa aaaagctata catgataggg gagttaaagg
     3601 ccaaaaggaa acaatcaagt caatgcaagc ttatttaatg caaacaattc taactgaact
     3661 tgaagaaaca catgcccttt atatgcgccg taaaaacatg aaacaatata atatctttaa
     3721 tcaataattc aaatgatttt ataacactcg aagagacatc aaaatattcc gttaaatcat
     3781 ctgacttttt aactgatgac gacgaaaaaa atcaagaaat tgtaagtgat ttagaaaaag
     3841 gtttgtatcg aaaacgtatg ttaagttatg gtggattgct taaacaaaag cataaaattt
     3901 taaacttaga cgatgccgaa gatggtaatt tgattaatac aagtgatgaa gataaaacga
     3961 cagacgaaga aaaaaagcac attcaattac cgcaatttgg aatttcgaaa agcaaaatta
     4021 ttatttaaga cattagtgat aactgatgtc tttttttatt gtttttctgt aaaaaagtgc
     4081 tgtcttattt ttgtgacaaa tgctgtatgt agtgtcacaa aagtgtgaca ctacagcttt
     4141 gtatgatatc actttaaaat aacttaaaac ccttggaatg tctggccttg ccagatctat
     4201 catttttgaa tgatagcaaa tttcccttat gctcttacgg agattttaga gttaaattaa
     4261 aatttctaag tttttgataa gaaaagccct gcaggaattt agaaaaacat ggggaagttt
     4321 taaagtattt tatgctaatt tttaaatttg catgtaaatg ggcagtatct aaaaaattag
     4381 tcactgattt tgctgaaatt tttgtttgtg aaattagcat atatttattt ggctcatatt
     4441 tgcgttttaa gagactgtat agatttttaa gtacaaaacc tatatgattt gcctctaagt
     4501 cttcaaaatg cctcttaaaa tccaaaataa aagtgtttaa tattgtaata aggattgcta
     4561 tttttataaa aggagagata tcatgaagat taaaactttc gctactttta ctcatcgtca
     4621 attagataaa caagttaata ggtttttaaa gaatgatatt catgtattag aaattcaaac
     4681 acacagtaat tggttatata tttcagctat tgttttttat gaggataaat aaggaacatc
     4741 gtatttatgc cgaaaaaatt tattgatgtt gagaagaacc cttaactaaa cttgcagacg
     4801 aatgtcggca tagcgtgagc tattaagccg accattcgac aagttttggg attgttaagg
     4861 gttccgaggc tcaacgtcaa taaaacaatt ggaataaaat caacctacag agcatccagc
     4921 tgcaaatgca gttccgcaaa ctgcttcaca tacaccgccg gctattggat ttacgattgc
     4981 ccatactcca cagtagtgag cacatgcata aagtccagcc atactacaag cgaatgcttt
     5041 gccgttccat ttgaatcctg catatgttac cggtctgttt ttatcaactt tttcagcctt
     5101 atattcaacg taatttttgt taataagtat ttttccctct ttgttttcag tcatacttct
     5161 tttttcggca atgaattttg tgattttttt gttgtagtta tcatatattg tttcagaaac
     5221 tacaatttct tttgtttttt catttttata agcgttatta gctatgtagc catatgcatt
     5281 tttatcattt gcttctgtag ctagatattt taatttagaa tttttgtctt ctagctttat
     5341 ttttttaaat ccttcttttt tcatttgctt ttcgaatttt tgcatatctt catttttatt
     5401 ttgcaattgt tctagaaaac ttatttcttt cttggtcatt tgtgatactt cgatgttttt
     5461 gcttttcaat ttttcttttt gattttctag taaagaactt ttttgttctt catttgcgta
     5521 agcattagaa aaattcgcgc taaataccag taggaacaag gaaattattg aaaataattt
     5581 tataattttc atttgatacc tccaagataa taagatattt taattgtata agtaagcgct
     5641 ttataaaatc aatatttatt ttaatatatt tataaacata tttttgaaag gagtctattt
     5701 tatgaatgaa aatttagaaa aatatataaa aagtttgcca cttttaggtt tgttaattag
     5761 tattttgtta attgttttat tcttttttat tttagatgtt gatgaagatt tattcgtggt
     5821 ttttttatac tgtttattgc ctttgatagt taatttgtct atttatggtt tttatgtttt
     5881 tgttaaaaga aagtaagatt atgaataatt ttgtttttaa tttgtatgct ttgagggggt
     5941 tggaaaacgg taggtgtata gattagattt cttgtttttt tgaaacgagt gtgaacgagt
     6001 ttcttcttgt cttgattttt gataaaaacg ccctacagga atttagaaaa acgtggggaa
     6061 gttttaaaga attttatgct aatttttaat ttgcatgtaa atgggcagtg tctaaaaata
     6121 gttattgttt ttgctcaaat ttttgttttt gaaaatagca tatatttatt tggctcatat
     6181 ttgcgtttta aaagcatata taagttttag atataaaact atatgaatca cccctaaatc
     6241 tttaaaatgc cctcttaaaa ttcaaaataa gggcataaaa aataaaagct accccagata
     6301 aagtgaggta gctttttctt tcatacggtc tatttatttt tctttcgacg ccgtatcgtc
     6361 gatgtaattc aaaaaagtta tgggctataa atctacatca ctttttcaag aatgtagtag
     6421 caatattcaa cgcaaattaa tttttataac tgctcggaat atctcaagcc gtttctttaa
     6481 ttttgaaata aaaaaatcga cgaaggtcga ttacgttttt gcacatgtcg atttatccga
     6541 cgtataagtg cgcccttacg ggatttaact agattataac gacgaatttt agacctgtaa
     6601 agcaataaca attaggtcta ccaggtagat aaagtgtaca aagtagaacc gaaaaagtta
     6661 tttattttga agatttttcg tagatataat ttgctaataa acttcctaaa actgaataaa
     6721 gaaaaatagt taaactcatg taatcactcc ataattctga agtgtctcca tgtctacgca
     6781 tagacacgca tttatgccga aaaaatttat tgatgttgag aagaaccctt aactaaactt
     6841 gcagacgaat gtcggcatag cgtgagctat taagccgacc attcgacaag ttttgggatt
     6901 gttaagggtt ccgaggctca acgtcaataa agcaattgga ataaagcacc tatgattatt
     6961 taaaaatata agttataata cattcaaaaa tatttttgaa tgaggtgtat tatgattcaa
     7021 acggttgttg ctgctgctgt tctttatatt gctacagcag ttgatttatt agtaatttta
     7081 ttaatatttt ttgctagagc aaagactaga aaagaatata gagatattta tgttggtcaa
     7141 tatttagggt ctattatttt aatattagtt agtttgtttt tagcttttgt attaaattat
     7201 gttccagaaa agtggatatt aggtttattg ggtttaatac caatttatct tggaattaaa
     7261 gtggctattt atgatgattg tgaaggagaa aagagagcta aaaaagaatt gaatgaaaaa
     7321 ggattgtcta aattagttgg tacggttgca attgttacga tagcaagttg tggcgccgat
     7381 aatattggtt tatttgttcc gtattttgtg acattaagtg ttactaattt attacttact
     7441 ctgtttgtct ttttaatttt aattttcttc ttggtgttta ctgcacaaaa attagctaat
     7501 attccaggaa ttggagaaat tgttgagaaa tttagtcgtt ggattatggc tattatttat
     7561 atagctttag gtttatttat tattattgaa aatgacacta ttcaaacaat tttaggattt
     7621 atattttaat ttagggtgtg attttatatg agttatgaaa atacttgtga tgtgatctgt
     7681 gtacatgagg ataaagttaa caatgcttta agttttttag aagatgataa atctaagaaa
     7741 ttacttaaca ttttagaaaa aatttgtgat gagaagaaat tgaaaatcat attatctttg
     7801 attaaagaag atgagttgtg tgtttgtgat atttctttga tattgaaaat gagtgttgct
     7861 tcaacttcac atcatttaag gcttttatat aaaaatgagg tacttgattt ttataaagat
     7921 ggaaagatgg catattattt tattaaagac gatgaaataa gagagttttt ctctaaaaat
     7981 catgagggtt tttgaaacga gtttcttctt gtcttgatac tataaaaaac aaagttatat
     8041 aacataagac gacttatcgg aagtagatat gcttgtatat tcacagatgg tataataata
     8101 gagtcgccta tctctcaggc gtcaattgac gaagagagga ggtgcattac tttgctaatc
     8161 attttcgttc acatcatagc accagtcatc agtggctgtg ctgttgcgta ttatacttat
     8221 tggcttagta aacgcaacaa ataattaata ggcgacttta taagccagca tcgaaaaaat
     8281 ccccacacta ctgcaatagt gtggggattg gtgcattaat tgttaatcat tttcgttata
     8341 taaattataa cactggttag tagagaaatg caaaatgatt gaattaatag taaatgtatt
     8401 aatgaatact cacacttata cttttaaaat agttcttcct aaatgttgtc cttttaaaag
     8461 ctgatctatc gttttataga attcttcaaa tccaatttca ttgtatagaa gtgaatttcc
     8521 tatattccat tcatttgcta acttactcca tatatcttca cggatattat gatcaacata
     8581 tacagaatca atgccaatta atttaatacc tctaagaata aacggtaata tattagcgtt
     8641 aagtgaattt cctgctacat ttccacatac agtagcaaca ccttgataat ttagacgttt
     8701 taaaccgtat aatgttacat tactccccac tgtatctaag aaataatcaa aaataccttt
     8761 tttaagcggt ttattatcat cttcaattat tattacttgt tttgcaccta atttttttgc
     8821 gatttctatt tgcttttctt ttcttacgat agcagtgata ttttcatatc caattttatg
     8881 taaaatttgt atagcaacac tgcccacacc acctgtagcc ccagacacta aaatttcagg
     8941 ttgtgatttg atattcattc catatttttc tagttcaaaa attgataatg ctgccgtaaa
     9001 accggctgtg ccatatacca tagactcttt aatagacatt tcttggggaa taggaacaat
     9061 ccactctcca ggcacttgtg catattctga aaatccacct gtatgtgtca cgccaacatc
     9121 ataaccagta actaatacat tatcgccttc tttaaactta tcattcgaac ttgattcaac
     9181 aatacctgca aaatctatac caggaatcat aggataatct ctaattacac cgcctttata
     9241 ttgtaaagct agcatatcct tataatttat tgaagagtat accactttta ctaatacatc
     9301 acccttcgat aaatgagatt catcaacttt ttctacttta taactaacat ttgatgcact
     9361 ttttctcact actaccgcat tgaacattta ttagccttct ttcacaattg ttttgtagaa
     9421 gtatgttttt gttgcaaaac tattttccta caaaacttat catgatatga taagcgagtc
     9481 aatactaatg atttaagaga ggttttagtt gtggaattag ataattgtat aaattatttg
     9541 ttgagtactt ctcagaatag agtttttaaa cattttaaaa gtttattaag tccctataat
     9601 attactccag cagaatatgg tgttttgaat tgcttgatta atagtaattt gaatacacct
     9661 aaagcgattg gtaatacttt aaatttagaa ccttcaacaa tatctggaat attagataag
     9721 atgtccaaaa aggatctaat aacacgtcaa actgataatg aaaatagaag aactgtgcta
     9781 ataaacagta caaataaagc aaaagactta tggccaacat tagaaaagaa agcctatcaa
     9841 cttaataatc attacttcgc taatttaact aatgaagaaa ctgtagtgtt taaaaagata
     9901 ttactaaaga taaataaaac tacgttttaa tttttacgtt ataaaaaagt tgaagtgcga
     9961 attataatcg agtaaatata aatgatagct tagcttaatt atatattaaa gccaactata
    10021 cttttattcc cgaaaaacct actatccaaa tttctaagat actttagcct ctataataaa
    10081 catattgtaa tataatttat gtattattta ggtttaccct aatcgaacaa taaaaaatac
    10141 ttgtaaaatg ctattgtcac tttgcttgat atatgagaat tatttaacct tataaatgag
    10201 aaaaaagcaa cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat
    10261 taaactattc atctattatt tatgattttt tgtatataca atatttctag tttgttaaag
    10321 agaattaaga aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat
    10381 tatacatgtc aacgataata caaaatataa tacaaactat aagatgttat cagtatttat
    10441 tatgcattta gaatattaaa attccttcat tacactcttg gcggtttcac ttatcaactt
    10501 atcatttggc ttatcacttt tattgtcttt attcgtaaaa atgactaaaa caataggttc
    10561 agattggccc ttaggataaa caaaagcaac atcatttcta gaagcatatg ttattgcttg
    10621 accactttta tcagcaacct tatagtcttt tggaacacca tctttaatta aagtgtctcc
    10681 gtttttatta ttaaacatta aatcaagtaa gaaattttta ttttttttgc ttaattttcc
    10741 atttgcgata agtttattta aagtcttgcc gaaagcagca ggcgttgaag tatctttttt
    10801 gctctttggt gaatagtaat ttaattctat ctcatatcta actggatttg ttactttatc
    10861 tcctagttct tttagacgtt gtttaacttt tttgattcca ccgatttctt ttataatttt
    10921 attgtttgct gtattatcac tatatgtcat tgaagcctca ataagttctt ttaaagtgat
    10981 atcttttcct acatattttt ctaaaatagg agaataagca actatatcat ctttgttaat
    11041 atgtactttt ttatttaact tattataagg tacttgttct aacaaaatag cactatttat
    11101 cgcttttgaa gtcgaagcat aggcaaatct cttatctgaa ttaaatttta cttccttacc
    11161 acttttagta tttaaagcat aaacaccaat atgagcatta tatttttttt ctaaatcatt
    11221 taactctttg gcatgtgaac tgtttgaatt acatgcactt aaaactaaag caattacaat
    11281 taaaaatatt aactttttca aaataaaccc tccgatatta cagttgtaat ttttataaat
    11341 caataatatt acaattgtaa tattgatgtc aattgtttat tataaatatt tgaaagaagg
    11401 tgtcaaaatg gctaaattac taataatgag cgtagtaagc ttttgtttta tatttctatt
    11461 gttagtattt tttaggtaca tattaaaacg ctattttaat tatagtttaa attataaagt
    11521 ttggtatcta actatgcttg ctggattaat tccttttatc ccaattaaat tctcttttat
    11581 aaaatttaat aatgtgaata atcaagcgcc cacagttgaa agtaagtcac acgatttgaa
    11641 ccataacata aataccacca aacctattca agagttcaca acagatatcc ataagtttaa
    11701 ttgggattca attgataata tctgcacagt tatttggata gttttagtta ttattttaag
    11761 ttttaaattt ttgaaagcct tattatatct taaatattta aagaaacagt cactttatct
    11821 aaacgaaaat gaaaaaaata aaatagatac gatacttttc aaccatcaat ataaaaaaaa
    11881 tattgtgatt cgaaaagcag aggctattca atctccaata actttttggt atgggaaata
    11941 tattattttg attcctagtt catattttaa aagtgtaatt gataaaagac taaaatatat
    12001 aatactgcat gaatatgctc atgctaaaaa tagagatact ttacatttaa ttatctttaa
    12061 tatcttctct attattatga gttacaatcc attagtacat attgtaaaaa gaaagataat
    12121 tcatgacaat gaagtagaag ccgatagatt tgtccttaat aatattaaca aaaatgaatt
    12181 taaaacttat gcggaatcta ttatggactc cgtattaaaa accccatttt ccaacaaaaa
    12241 tatattaagc cattcattta atggtaaaaa atcattactc aaaagtagat taattaatat
    12301 aaaagaagcc gatctgaaaa agcagtcaaa attaattcta atttttattt gtatttttac
    12361 tttttttatc atgataattc aaagccagtt tttaatgggg caatccttaa ctgattataa
    12421 ttataaaaag cccttacaaa gcgattacca aattttagat gaaagtaaaa attttggttc
    12481 aaatagtgga tctttcgtta tgtacagcat gaagaaagac aaatactata tttataatga
    12541 aaaagaaagt agaaaaagat attctcctga ttcaacttac aaaatttatt tagcactgtt
    12601 tggacttgac cgtcatatta taagtgataa aaattcacgt atgtcatgga atcataaaca
    12661 ttatcttttc gagtcttgga ataaggaaca agatttaaat acagcaatgc aaaattcagt
    12721 taattggtac ttcgaacgta ttagtaatca aataccaaag aactatactg cggctcaact
    12781 caagcaatta aattatggta atgaaaattt gggaagttat aaaagctatt ggatggaaga
    12841 tagtttgaaa atatctaatc ttgaacaagt aatagttttt aaaaatatga tggaacaaaa
    12901 taaccatttt agtaaaaaag caaagaatca attatcttct tcattattga ttaagaaaaa
    12961 tgaaaagtat gaactgtatg ggaaaacagg tacaggtata gtaaacggga agtataataa
    13021 tgggtggttt gtaggttacg taattacaaa tcatgataag tattattttg ctacacattt
    13081 atcagatgga aacccgtctg ggaaaaatgc tgaattaata agtgaaaaaa tattaaaagg
    13141 aatgggtgtt ttaaatgacc aat
//
