LOCUS       CP030460                8041 bp    DNA     circular BCT 10-OCT-2019
DEFINITION  Staphylococcus aureus strain ER04013.3 plasmid unnamed2, complete
            sequence.
ACCESSION   CP030460
VERSION     CP030460.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN09484035
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Sullivan,M.J., Altman,D.R., Chacko,K., Cliferri,B., Webster,E.,
            Deikus,G., Lewis,M., Khan,Z., Beckford,C., Rendo,A., Samaroo,F.,
            Sebra,R., Karam-Howlin,R., Southwick,K., Adams,E., Ying,L.,
            Kornblum,J., Factor,S., Danesh Yazdi,M., Dingle,T., Hamula,C.,
            Bashir,A., Schadt,E., Kasarskis,A., Patel,G., Wallach,F., Gibbs,K.
            and van Bakel,H.
  TITLE     Whole genome sequencing to identify and define MRSA outbreaks
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   Sullivan,M.J., Altman,D.R., Chacko,K., Cliferri,B., Webster,E.,
            Deikus,G., Lewis,M., Khan,Z., Beckford,C., Rendo,A., Samaroo,F.,
            Sebra,R., Karam-Howlin,R., Southwick,K., Adams,E., Ying,L.,
            Kornblum,J., Factor,S., Danesh Yazdi,M., Dingle,T., Hamula,C.,
            Bashir,A., Schadt,E., Kasarskis,A., Patel,G., Wallach,F., Gibbs,K.
            and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2018) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 296.360967216x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2018 19:16:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,089
            CDS (total)                       :: 3,010
            Genes (coding)                    :: 2,927
            CDS (coding)                      :: 2,927
            Genes (RNA)                       :: 79
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 41 of 83
            Pseudo Genes (incomplete)         :: 42 of 83
            Pseudo Genes (internal stop)      :: 19 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ER04013.3"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2015"
                     /collected_by="Mount Sinai Hospital"
     gene            complement(425..859)
                     /locus_tag="DQV54_15240"
     CDS             complement(425..859)
                     /locus_tag="DQV54_15240"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QFJ82505.1"
                     /translation="MRNNRNMDERQEIINKNMLIRSFGFLFILLIIYIAFLGVINVTIT
                     GHQLILISIITLTSIYMWIDSFINKILYYDVQNNKEIKRKLSSCVTTLLVIDVAVLILA
                     FLNKIDINISFLFLAILISFNIIVLSVYYIILKFWLIWYK"
                     /name="hypothetical protein"
     gene            complement(849..1055)
                     /locus_tag="DQV54_15245"
     CDS             complement(849..1055)
                     /locus_tag="DQV54_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_847658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="QFJ82514.1"
                     /translation="MNNIKHYRKEMNFSQQELANKVEVTRQTISLIELGKYNPTIKLCI
                     SIAKALQVDLNKLFWEMSDNSEE"
                     /name="transcriptional regulator"
     gene            1716..2099
                     /locus_tag="DQV54_15250"
     CDS             1716..2099
                     /locus_tag="DQV54_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_863254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="QFJ82506.1"
                     /translation="MSEQNIFVASDTVNGQIHKPNRKAPKQISFRVSESEYLKLKQSAE
                     TLNMSVPAFVKKKAQGTRLVVPKLDKTTRQSLASDLGKLGSNANQIAKYCNQHQYDAPN
                     YEALERNISELRERLDDIWQQLN"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            2081..3088
                     /locus_tag="DQV54_15255"
     CDS             2081..3088
                     /locus_tag="DQV54_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rlx"
                     /protein_id="QFJ82507.1"
                     /translation="MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASRE
                     LYGKTDGNQGHVIIQAFKPDEVTPEQCNQLGLELAEKFAPNHQVAVYTHNDTDHVHNHI
                     VINSINLETGKKFNNNKQALRDLRNFNDEVCRNHGLSVPEKDTARLRYTQTEKAIADPN
                     IKSTAQYSWKDEIREAIDQSQATNIDELKTHLSQYGIEIERVTPKSITYRHLAEDKKVR
                     GRRLGENYNKGGIEDGFERQIQRRQQERDSEHDYQPKRRTSNRDESTERDTSITQSDWD
                     RFAKDTNELERSRKAAESARLAHEKARRDREERIQAERATRKNINNDRDHGYEL"
                     /name="protein rlx"
     gene            3129..3605
                     /locus_tag="DQV54_15260"
     CDS             3129..3605
                     /locus_tag="DQV54_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QFJ82508.1"
                     /translation="MKHYIQRIDSDNLKQDFKTAMQEELKDIKSETENMVKQFQVSQIE
                     LQTSNNEYAKTIEARLNKNETAIKQYDQAVNKLTKGLTSMFFIVALVMVACLVLSPLSD
                     VLGVHYFYEWINHVLKTGHSAWRYFIVILYLIPYILLWLLIRGILSAYKRVSSF"
                     /name="mobilization protein"
     gene            complement(3919..4134)
                     /locus_tag="DQV54_15265"
     CDS             complement(3919..4134)
                     /locus_tag="DQV54_15265"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QFJ82509.1"
                     /translation="MVTKSCLYCYKKLPLKSQMHCPSKVQTPPKKGFKKIYKKILKSSG
                     QFSPHKFKRQKGKQHKVVAIYQIQVL"
                     /name="hypothetical protein"
     gene            4327..5184
                     /locus_tag="DQV54_15270"
     CDS             4327..5184
                     /locus_tag="DQV54_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="QFJ82510.1"
                     /translation="MSGETVVYQNEMNLVPLRKFTSTEIDIFFSLCNKLKEQDIKELAI
                     PFEELRHLSNYYHRSLKRFVKDLEHVYDKMLTLTYTERSGLSFKKFVLFTGYEVDVDEK
                     NLIVSINPKLKHVLNEITADFTKFELKEMTNLKSTYSKNMFRLLKQYKHTGYFKIQIED
                     FRERLDIPNSYRMTHINQKVLAPIIKELGFIFNNLNINKVKAKKGRKIEWLEFTFDAEK
                     RIHNKREPQMANIGKSRQYISREKTPKWLEERTYEKTPQREYDPQLEKEREAFLKQLEM
                     NWEE"
                     /name="RepB family plasmid replication initiator protein"
     gene            complement(5326..5421)
                     /locus_tag="DQV54_15275"
     CDS             complement(5326..5421)
                     /locus_tag="DQV54_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="QFJ82511.1"
                     /translation="MTLLFVYVIAPVFVGCCITLFQFWLEQRSKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            5570..5755
                     /locus_tag="DQV54_15280"
     CDS             5570..5755
                     /locus_tag="DQV54_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QFJ82512.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIHFLNKNGEWYYFEVRSVWRGRHII
                     RVKDGLLGWRKEIVTE"
                     /name="hypothetical protein"
     gene            complement(6000..7715)
                     /locus_tag="DQV54_15285"
     CDS             complement(6000..7715)
                     /locus_tag="DQV54_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="QFJ82513.1"
                     /translation="MHKSNPLLSILKNLKFPKLLLLSALIISSIGSIAGLLVPWFTGEL
                     VNKINGNSLSFNFLVIVISIFLLNAIISGVGLYLLSKIGEKIIFSIRCLVWNHIVRLKT
                     SFFDSNESGELLSRIIDDTKVINTFVSHKLPSTLPNILTIIGSIIMLFILDWKMTLCTF
                     VIVPLFFMIILPLGQMMEKTSNKTQNEIAQFSGMISRILSEIRLVKVSSTENLEVKNGK
                     DRLTNIYNLGIKQAKITSIIEPISSILVLLMIAIVLGFGGWRVSSGNISSGNLVAMIFY
                     VFQLVLPISNISTLITDYKQAKGASFRLSEILSQKTEEIEGKRNEDLDIPFIKFENVTF
                     SYDKEEILKNLSFTIPQNKVTAFVGPSGSGKSTIFNLIEKMYDIDNGKIYIGNSLLSDL
                     DLLSWRKNLGYVMQDNSMMSGTIRDNILYGLEDQEIKDDYLDYYCKLANCYDFVNNLEE
                     RYNTFIGERGVKLSGGQRQRIDIARSFIKNPKVLLLDEITANLDSESEHKIQDAMSTLM
                     RDRTTIIIAHRLSTIQKADQIIFLDNGVITGMGDHNSLYNSHKKYKEFVLNQHI"
                     /name="ABC transporter ATP-binding protein"
     Typing          complement(1508..1745)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.25"
                     /coverage="100.0"
                     /evalue="9.95e-107"
     Typing          4328..5184
                     /name="rep_cluster_2222"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.909"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2121..3001
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.837"
                     /coverage="87.0"
                     /evalue="1.16e-168"
ORIGIN
        1 aattctataa ataattttcg aggaggtgtt ttcatgggtg ctgtagcaaa atttcttggt
       61 aaagcagctt taggtggtgc tgctggtggc gcaacttacg cagggttaaa aaagattttt
      121 ggttaatatt taaaaaattt aaggagttat ttacatgggt gcattaatta aaactggtgc
      181 taaaatcatt ggtagtggtg ctgctggtgg tttaggtaca tatattggtc ataaaattct
      241 aggtaaatag cgtttactta aaaagcaaag gagagaaaac tatgggcgca gtaattaaag
      301 taggtgctaa ggtcattggt tggggagccg ctagtggcgc aggtttatat ggtttagaaa
      361 aaatttttaa aaaataacta agcattcgag tataaggttt aatcaaacct catactcgat
      421 ttttctattt ataccaaatt aaccagaatt ttaatattat ataatataca cttagtacta
      481 taatgttaaa ggatattaat atagctaaaa ataaaaatga aatattaata tcaatcttat
      541 ttaaaaaagc caatattaaa actgcaacat caataactaa caatgtcgta acgcatgatg
      601 acaactttcg ttttatctct ttgttatttt gaacatcata atacaaaatt ttatttataa
      661 aagaatcaat ccacatatat atactagtta gtgtaataat actgattaat attaattggt
      721 gtcctgttat agttacatta atcacgccta aaaaagctat ataaattatt aatagtataa
      781 ataaaaagcc aaaagaccta attaacatat tcttattaat tatttcttgt ctttcatcca
      841 tatttctatt attcctcact attatcactc atttcccaaa ataatttatt caaatctact
      901 tgtaaagctt tagctatgct aatacataat ttgatagtag gattatattt ccctaattca
      961 attaaagata ttgtctgcct tgttacttca actttattag ccaactcttg ctgcgaaaaa
     1021 ttcatttctt tacgataatg cttaatatta tttatcatga ttataaactc ttcctttttc
     1081 acacacactt ttgccatata tatattgcat ttataatgta acaaatctat attaattatg
     1141 caataccctc gtcattataa agttaaaatg agattacctt gaaaattaaa acgtattata
     1201 aacgtaagta tttagggtgt ttataaacta atgtttgatt atgggtaaaa tatataattt
     1261 atttgtttgc ttcaagtatt ttttgtattc tttataagaa tatggggtgt ctaatttttt
     1321 catcaatgta tactgcagga acttcctcgc tatatatcat tgttttttct tacttaacct
     1381 tatttctgaa atcaaattta tattttttga aatattaaac atttcagtaa ttagtattct
     1441 tgtaagaatt atttttaccg atatcaagaa aaaaagctaa acatctttaa atacatttat
     1501 attaatagtg gctagcaatt agccactatt ttttcgccag aaaaaatcct aaggggcttg
     1561 gggaatatcc ccaacaagca ggcgatgcta ccacgttagt ggctagcaaa gccaatgctt
     1621 gccaaaccac tataacgact tgattaaatt tactttatcc tataaagtaa atttatatta
     1681 aggagttatc tttcgtagaa agcgtggtgg gatgtatgag cgaacaaaac atatttgtgg
     1741 ctagcgatac ggttaatggg caaattcata aacccaaccg taaagcgcca aaacaaatta
     1801 gttttcgtgt gagcgaatcc gaatatttaa agttgaaaca atcggctgaa actttaaata
     1861 tgagtgtgcc tgctttcgtt aagaaaaagg cacaaggcac tcgattagtt gtacctaaat
     1921 tagataaaac tacacgacaa tctctagcta gtgatttagg taaattaggt tcaaatgcca
     1981 atcaaattgc caaatattgt aaccaacatc aatatgacgc accaaattac gaagcattag
     2041 aacgtaatat cagcgaatta cgtgaaaggc tggatgacat atggcaacaa ctaaactaag
     2101 cgcaaccaaa tcaacatctc gtgctattaa ttacgctgaa aaacgcgctg ttgagaaaag
     2161 tggcttaaac tgtgatgtag attatgctaa atcgagtttc aaagcgtctc gtgagcttta
     2221 cggtaaaaca gatggcaacc aaggtcatgt cattatacaa gcctttaaac ctgatgaagt
     2281 tacacctgaa caatgtaatc agctaggttt agaactcgct gaaaaatttg caccgaatca
     2341 tcaagtcgct gtctatacac acaatgatac ggatcatgta cataatcata ttgtgattaa
     2401 tagtattaat cttgaaacag gtaagaaatt caacaacaat aaacaagcat tacgtgactt
     2461 aagaaatttc aatgacgaag tttgtcgcaa tcatggttta agcgtaccag agaaagatac
     2521 agcacgctta cgttataccc aaactgaaaa agccattgcg gatccaaaca taaaatcaac
     2581 agcacagtat tcatggaaag atgaaattcg agaagctatc gaccaatcac aagcaacgaa
     2641 tatagacgaa ttaaaaacgc atttaagtca atatggcatt gaaattgaac gtgtcacacc
     2701 aaaaagcatt acgtatcgac acctagctga agataaaaaa gtacgggggc gtcgattagg
     2761 cgaaaattac aataaagggg gcatagaaga tggctttgaa agacaaatac aacgacgaca
     2821 acaagaacga gactcagaac acgactacca accgaaacga cgaacttcga atcgtgatga
     2881 atcaactgaa agagatacaa gcatcactca atcagattgg gatcgattcg ccaaggatac
     2941 aaatgaattg gagcgtagcc gaaaagctgc agaatcagca agacttgctc atgaaaaggc
     3001 tcgaagagat cgagaagaga gaattcaagc ggaacgagcg acacgaaaaa atattaacaa
     3061 cgatcgagac cacggctacg aactttgatc aagcgacaaa gactaatgaa aaacgattta
     3121 ttagcgcaat gaaacactat attcaacgca ttgatagtga taatttaaaa caagatttca
     3181 aaacagccat gcaagaagaa ctaaaagata ttaaatcgga aacagaaaat atggttaaac
     3241 aatttcaagt gagtcagata gaattacaaa catccaataa tgaatacgcg aaaaccattg
     3301 aagcacgctt aaacaaaaat gaaaccgcga taaagcagta tgatcaagca gtgaataagt
     3361 taacgaaagg attaacttct atgtttttca tcgttgcttt agtaatggtc gcatgtttag
     3421 ttttaagtcc gttaagcgat gtattaggtg ttcattattt ttatgaatgg attaatcatg
     3481 tactcaaaac aggtcattca gcatggcgtt attttatcgt tatcctttat ttaatccctt
     3541 atatcttatt gtggttgctt atacgtggca ttttaagcgc atataagcgc gttagttcat
     3601 tttgaatagg tagagaatca tatcaacgta tagaagttct acatgaagct gaaaatggat
     3661 tttaaaagca tttaaataat aatgactatt taaatccatc gttatgagaa gcgtattaaa
     3721 gattagatat cgctattatg ccgagaaaat ttattgatcg ttgagaagtc cccttaacta
     3781 aactgtttcg acgaatgtcg ggtatagcgt gagctattaa cccgatgagt cgacagtttt
     3841 tgggtttgtt aaggggacag aggctcaatg tcaataaagc aattggaata atacatcaat
     3901 gagtaacgac ttaatgattt ataatacttg aatttgataa atagcaacca ctttgtgttg
     3961 ctttcctttt tgtctcttga atttgtgagg ggaaaattgc cccgaacttt ttaatatctt
     4021 tttataaatc tttttaaaac cctttttagg gggtgtttga accttagagg gacaatgcat
     4081 ttgagatttt aaaggcaact ttttgtaaca ataaaggcaa ctttttgtaa ccataaaggc
     4141 aactttttgt aacaataaag gcaacttttt gtaacaataa aggaaacttt ttaatcgcga
     4201 gttgccttta ttgttacaat tatgctatta taggttttaa ttgaattttt cttatattat
     4261 taaaagacat cttattatta ataaagtgtc ttttaatcca cttattcttt aggaggtttt
     4321 ataaacatgt ctggagaaac tgttgtatat caaaatgaaa tgaatttagt tccattacgt
     4381 aagtttacca gcacagagat tgatatattt tttagtcttt gtaataagtt aaaagaacaa
     4441 gatataaaag aattagctat cccttttgaa gagttacgtc atttaagtaa ttactatcat
     4501 cgctcattaa aacgttttgt taaagactta gaacatgtat acgataaaat gttaactcta
     4561 acttatactg aaagaagtgg tctatctttc aaaaaatttg tattatttac tgggtatgaa
     4621 gttgatgttg atgagaaaaa tttaatcgtt agtattaatc ctaaattaaa gcatgtttta
     4681 aatgaaataa cagctgattt tactaaattt gaattaaaag aaatgacaaa tcttaaatct
     4741 acatactcta aaaatatgtt tagattatta aaacaatata aacacacagg ttattttaaa
     4801 attcaaattg aagattttag agaacgatta gatattccta atagttatcg catgactcat
     4861 attaatcaaa aagtattagc tcctataatt aaagaattag ggtttatctt taataacctt
     4921 aatatcaata aagttaaagc caaaaaaggt cgtaaaattg aatggttaga gtttactttt
     4981 gacgctgaga aacgcatcca caataaacga gaaccacaaa tggcgaatat aggtaagtca
     5041 cgacaatata tcagtcgtga gaaaacacca aaatggttag aagaaaggac atatgaaaaa
     5101 acaccccaaa gagaatatga tccacaacta gaaaaagaaa gagaagcctt tttaaaacaa
     5161 cttgaaatga attgggaaga ataaaaatgt attaaacgat aaataataat tgaagaatgt
     5221 gtaataaaat gatactattt agacaacaaa tagtggtgtc attaacgcac cgaaccccca
     5281 acctatggac gtaggttggg ggttctttta gtgttggctt gtcgcctatt ttttactacg
     5341 ttgttctagc caaaattgaa acaacgtaat acagcatcct acaaaaacag gagctattac
     5401 ataaacaaat agtaatgtca tttagcgcac ctcctctcta cgtcaaatcg acgtctgaga
     5461 gatagacgac tttcacatta taacaaatct ctccttccat gaaataacat gaatattaat
     5521 aaatgttgtt atatattaaa gataaattaa tataagggtg ggattgatga tgtttggcga
     5581 tcaaagacaa gaagctacaa aatatgtcat taaagaaggt tatcaagata ttcatttcct
     5641 caacaaaaac ggggaatggt attatttcga agtaagaagt gtctggcgtg gcagacatat
     5701 tattagagtt aaagatggtt tgttaggttg gagaaaagaa atcgtaactg aataaagata
     5761 caaaaactta agttatgaaa aagaatgaaa acgtgtcatt tagggtaatg tataaaagat
     5821 gaatacccgc taagattgat aaaaagcccg aaacactttc atatcagtgt ttcgggcttt
     5881 tcttttagtt aacataatgt tcaattatgg gtagttgttt cgtatatagt attcactgta
     5941 ttaattttat tctgatcact caatataaca aagtgtatat atcagatata tacactttgt
     6001 tatatatgct ggtttaacac aaactcttta tactttttat gactgttata taatgaatta
     6061 tggtccccca tacctgtgat tacaccgtta tctaaaaata ttatctgatc agccttttgg
     6121 attgttgata gcctatgtgc aataataatt gttgttctat ctctcattaa agtagacatt
     6181 gcatcttgaa ttttatgttc actttcacta tctaaatttg cagtaatttc atcaagtaat
     6241 agtactttag ggttttttat aaaacttcta gcaatatcta ttctttggcg ttgccctcca
     6301 gataacttga ctcctctttc tccaataaat gtattatacc tttcttctaa attattaaca
     6361 aaatcataac aatttgctaa tttacagtaa taatctagat aatcatcttt aatttcttga
     6421 tcttctaatc cgtataaaat attatctcgt atagtgccac tcatcattga gttatcttgc
     6481 attacatatc ctagattttt tctccaactc aataaatcca aatcactcaa taatgaatta
     6541 cctatatata tttttccatt atcaatgtca tacattttct caatcaaatt gaatatagta
     6601 ctttttccag atcctgaagg tccaacaaaa gctgtcactt tattttgtgg tattgtaaaa
     6661 cttaaatttt tcaatatttc ttccttgtca tatgaaaaag taacattttc aaatttaata
     6721 aaaggtatat ctaaatcttc atttctttta ccttctattt cttcggtttt ttgacttaat
     6781 atttctgata gcctaaagct agctccctta gcttgtttat aatcagttat tagtgttgaa
     6841 atattactaa taggtaaaac taattgaaat acataaaaaa tcattgctac taaatttccc
     6901 gatgatatat tcccacttga tactctccag cctccaaacc ccaagacaat agctatcatc
     6961 aataaaacta aaatgctcga tataggttct attatagatg ttattttagc ctgctttatt
     7021 cctaaattat aaatattagt taatcgatcc ttaccatttt tgacttctaa attttctgta
     7081 cttgaaactt taactaatct aatttccgat aaaattctac ttatcattcc actaaattga
     7141 gcaatttcat tttgtgtttt attagaagtt ttttccatca tttgtcctaa aggcagtata
     7201 atcataaaaa ataaaggaac aataacaaat gtgcatagag tcattttcca atctaatata
     7261 aaaagcataa ttatcgatcc aataattgtt aatatattag gtaaagtgct aggaagtttg
     7321 tgtgatacaa aagtatttat tacttttgta tcatctatta ttctacttaa tagctctcca
     7381 ctttcatttg aatcaaaaaa agaagttttt aatcgaacaa tatgattcca tactaaacat
     7441 ctaattgaaa aaatgatttt ttcaccaatt ttacttaata aatataaacc aacaccactt
     7501 ataattgcgt ttaataaaaa tatagaaata actataacta aaaaattaaa acttagtgaa
     7561 tttccattta ttttatttac taattcccct gtaaaccatg gtactaatag accagcaata
     7621 ctaccaatag acgatataat taaagctgat aataatagaa gtttaggaaa ctttaagttc
     7681 ttcaaaatac ttaataatgg gttactctta tgcaaattag tcactcctat aattacaaaa
     7741 ttaaaagcac atgttactta aaccgaataa acccatagca acatatgctt tatcataaat
     7801 tatttgaaaa tacataatga tagttatgta taataataat atcaatatat agtttttaca
     7861 atacctaatt attttatcaa taaatatctt gatattaaaa caaactaata ataaaatatt
     7921 aacaagagaa aaaagggagg tgttacaatg gggaaattag ctatcaaagc tggaaaaatc
     7981 attggcggtg gtatcgctag cgctcttggt tgggctgctg gagagaaagc tgtaggtaaa
     8041 t
//
