LOCUS       CP027495               11044 bp    DNA     linear   BCT 19-MAR-2018
DEFINITION  Staphylococcus aureus strain ST2594 isolate 004 plasmid unnamed10.
ACCESSION   CP027495
VERSION     CP027495.1
DBLINK      BioProject: PRJNA436877
            BioSample: SAMN08634544
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11044)
  AUTHORS   Bastos,L.R., Martins,M.C.F., Albano,R.M., Marques,E.A. and Leao,R.S.
  TITLE     Whole genome sequencing of ST2594 MRSA strain causing skin
            colonization and low-grade infection in surgical site. Implications
            for MRSA pathogenesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11044)
  AUTHORS   Bastos,L.R., Martins,M.C.F., Albano,R.M., Marques,E.A. and Leao,R.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2018) Departamento de Microbiologia, Imunologia e
            Parasitologia, Universidade do Estado do Rio de Janeiro, Av. 28 de
            Setembro, 87, Vila Isabel, Rio de Janeiro, RJ 20551-030, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.5; MAUVE v. 2.4.0
            Assembly Name                     :: Saureus141ST2594
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: BA000018.3
            Genome Coverage                   :: 40.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/06/2018 13:58:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.4
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,922
            CDS (total)                       :: 2,883
            Genes (coding)                    :: 2,764
            CDS (coding)                      :: 2,764
            Genes (RNA)                       :: 39
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 3 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 65 of 119
            Pseudo Genes (incomplete)         :: 37 of 119
            Pseudo Genes (internal stop)      :: 29 of 119
            Pseudo Genes (multiple problems)  :: 12 of 119
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Lab2_UERJ_19239.
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11044
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ST2594"
                     /isolate="004"
                     /isolation_source="Femural surgical wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed10"
                     /geo_loc_name="Brazil: Rio de Janeiro"
                     /lat_lon="22.8951 S 43.2431 W"
                     /collection_date="2015-03-11"
                     /collected_by="Leonardo Bastos"
     gene            complement(<1..857)
                     /locus_tag="C6V85_14425"
     CDS             complement(<1..857)
                     /locus_tag="C6V85_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_863213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="AVO70342.1"
                     /translation="MKNKEKYLTNFSEAKRKKATQKYNIIKPFILGEQSLSSISKSKGI
                     ALSTLYRWNKSYKEQGLKGLIYATRADKGTRKIEPKIIDEIERLALMNKRNSIATIHRK
                     ITNYCKENNFDIPSYKQIYSVIKAMPKSVIDFSHQGEKYYQNKYDLIQIRESSRPNEIW
                     QADHTLLDIFILDQKGNINRPWLTIIMDDYSRAIAGYFLSFDAPNAQNTALTLHQAIWN
                     KNDTNWPVCGIPEKFYTDHGSDFTSNHMEQVAIDLKINLMFSKVGVPRGRGKIERFFQT
                     VNQT"
                     /name="transposase"
     gene            complement(829..1422)
                     /locus_tag="C6V85_14430"
     CDS             complement(829..1422)
                     /locus_tag="C6V85_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="AVO70343.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTHGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKKDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEDKEDEKQGEVSN"
                     /name="HTH domain-containing protein"
     gene            complement(1595..2788)
                     /locus_tag="C6V85_14435"
     CDS             complement(1595..2788)
                     /locus_tag="C6V85_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="AVO70344.1"
                     /translation="MSKYYQIVIVGGGTAGITVASRLLRKNQSLKGKIAIIDPAEYHYY
                     QPLWTLVGAGVSSLKSSRKDMKSVMPEGVQWIKNAVSSFQPENNSIILGDNSVVSYEFL
                     VVAPGLQINWSSIKGLKENIGKNGVCSNYSPNYVRETWRQISKFKQGNAIFTHPNTPIK
                     CGGAPMKIMYLAEDYFRKHNIRSNANVIYETPKDALFDVGKYNKELEEIVEERDIIVNY
                     NYNLVEIDGDKKEATFEHIKTDDRKTIDYEMLHVTPPMGPLGVLKESTLSDSDGWVDVN
                     PTTLQHKSYSNIFGLGDASNAPTSKTGAAIRKQAPIVVNNLLQVMDNEMLTHHYDGYTS
                     CPIVTGYNKLILAEFDYNKKPKETLPFNQAKERRSMYIFKKDLLPKMYWYGMLKGLM"
                     /name="pyridine nucleotide-disulfide oxidoreductase"
     gene            complement(2806..4140)
                     /locus_tag="C6V85_14440"
     CDS             complement(2806..4140)
                     /locus_tag="C6V85_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="AVO70345.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIVIDPVRDLTKYMEVADSE
                     GFKITQAAETHIHADFASGIRDVAKRLNANIYVSGEGDDQLSYKNMPEHTNFVKNQDII
                     QVGNIKLEVLHTPGHTPESISFLLTDEGGGSSVPMGLFSGDFIFVGDIGRPDLLEKSVQ
                     MEGTTEIGAKQMYQSIEGVKNLPDYIQIWPGHGAGSPCGKALGAIPMSTLGYEKINNWA
                     FNVTDESKFVETLTSNQPAPPHHFAQMKKINQFGMKMYQPYNVYPSLDNERIAFDLRSK
                     EAFHGGHTQGTINIPYNKNFINQIGWYLDFEKDVDLIGDKSTVEQATHTLQLIGFDNVA
                     GYRLPKSEILTQSIHSADITGEEANVLDVRNEEEWNNGHLDQAVNIPHGKLLNENIPFN
                     KEDKIYVHCQSGVRSSIAVGILENKGFENVVNIREGYQDFPESLK"
                     /name="MBL fold metallo-hydrolase"
     gene            complement(4171..5238)
                     /locus_tag="C6V85_14445"
     CDS             complement(4171..5238)
                     /locus_tag="C6V85_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="AVO70346.1"
                     /translation="MKQYNENHINNFSKQELEKLGAQGQLIDVRTQEEYELAHINGATL
                     HPVDKIESFNKDKNTTYFVHCKSGTRSAKASEYLAKQGYDIVNLDGGYKAYEAKNDDVN
                     TFEENTNVEVKAERKQFNYSGLQCPGPIVNISKEVKNIEVGEQIEVTVTDPGFLSDIKS
                     WVKQTGHTLVKLDESNNGINAIIQKEKPKDLQVNHTAKGTTIVLFSGELDKAVAAMIIA
                     NGARAAGRDVTIFFTFWGLNALKKAQTVNVKKQGIAKMFDLMLPKTPLKMPLSKMNMFG
                     LGNIMMRYVMKKKNVDSLPSLINQAIAQDIKLIACTMSMDVMGITKEELRDEVEYGGVG
                     TYIGDTENASHNLFI"
                     /name="dihydroneopterin aldolase"
     gene            5376..5636
                     /locus_tag="C6V85_14450"
     CDS             5376..5636
                     /locus_tag="C6V85_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="AVO70347.1"
                     /translation="MNYDKKMINRINRIQGQLNGVIKMMEEEKDCKDVITQISASKSSI
                     QRLMGIIISENLIECVKAAEDNGENSEELINEAVNLLVKSK"
                     /name="metal-sensitive transcriptional regulator"
     gene            5636..6391
                     /locus_tag="C6V85_14455"
     CDS             5636..6391
                     /locus_tag="C6V85_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="AVO70348.1"
                     /translation="MDISTIIMMLLIGVFGGFISGLVGIGGAIIIYPAILLLPPLFGAP
                     AYSAYMASGLTSSQVFFSTLSGSLKARKKPEFSPLLVLYMGSGMIIGSMLGGLLANLFN
                     ATFVNTIYIIIALLALILMFINIKPSLDQSSFNKPLLVMVGLFVGIISGIVGAGGAFII
                     IPILLVLFKLPMNTVVANSIVIAFISSIGAFIIKLIQGYIPLEDALFLIIGSIIFAPLG
                     LKLGKKVPSFVQKWIISILIIIAIIQIVL"
                     /name="sulfite exporter TauE/SafE family protein"
     gene            complement(6562..7590)
                     /locus_tag="C6V85_14460"
     CDS             complement(6562..7590)
                     /locus_tag="C6V85_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance protein"
                     /protein_id="AVO70349.1"
                     /translation="MIDYLILVCRKVHLIKNFKWKRGAFIGILEKFQTLIILLAVGSGI
                     LLGQIGVIERYAETLVVPFLFLMLYGLFLTIPLHQLKKAFLNIRFLGSSTIINFIWTPV
                     LAWGLGAIFLSDHPALWIGFIMLMVTPCTDWYLAFTGIAKGNVSLSTSILPINLILQVV
                     LLPIYLLLFAGSIETIPLPTLIESILIVLIFPFVLAHLTRFLLRRKEPVLSNIVIPFFS
                     NAQIFFLSFAIMAMFASQGSYLLDHLEVIYILIIPILIFFIINYVLGRLVGRLLKFSYE
                     DTVSLSLTIIARNSPVALAIAVTAFPNQPLIASTLVIGPLIELPILAIVSQVLLFTKMS
                     GK"
                     /name="arsenic resistance protein"
     gene            complement(7538..7846)
                     /locus_tag="C6V85_14465"
                     /pseudo=""
     CDS             complement(7538..7846)
                     /locus_tag="C6V85_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020367071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            7770..8090
                     /locus_tag="C6V85_14470"
     CDS             7770..8090
                     /locus_tag="C6V85_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008179665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="AVO70350.1"
                     /translation="MNENKIVGNKKGLDEETLFVVSQTFKALSDPTRIRILNLLFLKEY
                     SVNEIAENLHLRQSTVSHQLRFLKNLRLVKYRREGTTLYYSHDDAHVMNMLKETINHAR
                     HH"
                     /name="ArsR family transcriptional regulator"
     gene            8153..9088
                     /locus_tag="C6V85_14475"
     CDS             8153..9088
                     /locus_tag="C6V85_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020361030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="AVO70351.1"
                     /translation="MSHEHNHTHGANKKTLMISFIIITGYMIIEAIGGILTNSLALLAD
                     AGHMLSDSISLGVGLLAFILGEKAADYSKTYGYKRFEILAAVFNGVTLVLIAIYIFYEA
                     YQRFTDPPEVASTGMLIIATIGLLVNILVAWILMRGGDTKENLNIRAAFLHVIGDLLGS
                     VGAIIAALMILFFNWGWADPLASVIVAVLVLISGWRVTKDAVHVLMEGTPKNVEIDAVI
                     KTIESIPGVNNMHDLHVWSITSGQNALSCHVVVDGTISIQESQEILRTIEDELEDENIG
                     HATIQMESENHPHKDSVMCRDDDITHSHEH"
                     /name="cation transporter"
     gene            9300..9725
                     /locus_tag="C6V85_14480"
     CDS             9300..9725
                     /locus_tag="C6V85_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AVO70352.1"
                     /translation="MKPHIIHFSGHGSSEHDIVLETAEGKPSFLSKEKVTLLMKTMSES
                     IKLVIFNNCFSNGQAEMVTEHVGFAIGMNEAIQDEAAKEFAAQFYSALGFGHTVQKAFE
                     QGKLALSLEGIEGDEIPELYSREGLDPNEHILVKPDF"
                     /name="hypothetical protein"
     gene            complement(10207..10302)
                     /locus_tag="C6V85_14485"
     CDS             complement(10207..10302)
                     /locus_tag="C6V85_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017176713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I addiction module toxin, Fst family"
                     /protein_id="AVO70353.1"
                     /translation="MHSILFELLIAPIIVGVIITLFAYWLDSRDK"
                     /name="type I addiction module toxin, Fst family"
     gene            complement(10638..10880)
                     /locus_tag="C6V85_14490"
     CDS             complement(10638..10880)
                     /locus_tag="C6V85_14490"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AVO70354.1"
                     /translation="MYHSNREKFGHKSYQEDINNYHQKGFDDEENEEEESLMDILDLLT
                     IDENRKLEMVFELIDDLDLEVINEMKVRIDNRLTH"
                     /name="hypothetical protein"
     gene            10954..>11044
                     /locus_tag="C6V85_14495"
     CDS             10954..>11044
                     /locus_tag="C6V85_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="AVO70355.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRD"
                     /name="IS6 family transposase"
ORIGIN
        1 aaagtttggt taactgtctg aaaaaagcgt tctatttttc cacgaccacg agggacacca
       61 acttttgaaa acatcaaatt tattttcaag tcaatagcga cttgttccat gtgatttgag
      121 gtaaagtcac ttccgtgatc tgtatagaat ttttcgggta taccacacac tggccagttg
      181 gtatcattct tattccatat tgcttggtgt agtgttaatg ctgtgttttg agcattgggg
      241 gcatcaaaac ttaagaaata gcctgcaatt gcacgactat agtcatccat aattatagtt
      301 agccacggtc tattgatatt acctttttgg tctaatataa agatatctag caaagtatgg
      361 tctgcttgcc atatttcatt gggtctcgag gattctctaa tttgtattaa atcgtactta
      421 ttttgatagt atttttcacc ttgatgagag aaatcaataa cagacttagg cattgcttta
      481 ataacactat aaatttgttt ataacttggt atatcaaaat tattttcttt acagtaatta
      541 gtgattttcc tatgaattgt ggctatacta tttcttttat tcataagcgc aaggcgttca
      601 atttcatcaa ttatctttgg ttctatttta cgtgttcctt tatcagctct tgtcgcatag
      661 attagtcctt taagtccttg ttctttatat gacttattcc acctataaag ggtgcttaac
      721 gcaatccctt tacttttaga aatacttgat aaagattgtt cgcctaggat aaaaggtttt
      781 ataatattgt acttttgagt agctttttta cgtttggctt cagaaaaatt agttagatac
      841 ttctccttgt ttttcatctt ccttgtcctc caatttattg aggtaacgat aaattgttgt
      901 acgtgaaact tgccattgtt ctgcaattgt tttaatgggg gtgccattat cataaagtgt
      961 tttgagtaaa tttaagtctt ttttattcaa tttttcaggt cttccaccat aacgtcctct
     1021 ggcacgagct gcaattcgtc cagctgaaga acgttctaaa attaaattcc gttcaaactc
     1081 tgcaaatgct gcgaataaat gaaataataa ttgtcctgta gaacttgatt tatccatcgt
     1141 tatattttct tctaaactat gaaaactcac gcctcgttcg ttaagttcat tcactaatgt
     1201 gattaaatcc tccatgttac gtcctagtct atctagtctc caaacaacaa tggtatctcc
     1261 tgacctcgca aattcaattg ctttatctaa accaggtctt ttactttttg aaccactcat
     1321 atggtcatta aatatttttt cacaaccatg tgtatttaat cgatcttcct gtaaatttaa
     1381 attttgtaat ccagttgaaa ctcttgcata acctattttc aaaatacaaa cctcctatat
     1441 acatcttcag tgtaccataa cttaatcaca aggtcttttg tgaacataga ttttttgttg
     1501 ggtttttgaa cacataattc tatgtaatac atacttattt agacataaaa agagagtgta
     1561 cagaaaatgg ttattttctg tacactcttg ttatttacat aagccccttt aacataccgt
     1621 accaatacat tttaggtaat aaatcttttt taaaaatata catacttctt cgttctttgg
     1681 cctggttaaa aggcaatgtt tcttttggtt ttttattata atcaaactcg gcaagtatta
     1741 atttattgta cccggtaacg atagggcacg aagtataacc atcataatga tgcgtcaaca
     1801 tttcattatc cattacttgc aataggttat taacaactat aggcgcttgt ttacgaattg
     1861 ctgctcctgt tttggaagta ggtgcatttg aagcatcacc aagcccaaat atgttagagt
     1921 agcttttatg ctgtaatgtg gttgggttaa catctaccca accatcacta tctgaaagtg
     1981 tactttcttt aagtacacct aagggaccca tgggtggtgt tacatgtaac atttcataat
     2041 ctattgtctt tctatcgtct gttttaatgt gttcaaatgt agcctctttt ttgtctccgt
     2101 caatttcaac aagattataa ttataattga caattatatc cctttcttca acaatttcct
     2161 ctaattcttt attatatttg cctacgtcaa ataaagcatc ttttggcgtt tcgtatatca
     2221 cattagcatt agaacggata ttatgttttc taaaataatc ttcagctaaa tacataattt
     2281 tcataggtgc gcctccacat ttgataggcg tattaggatg cgtgaaaatc gcgttccctt
     2341 gtttaaattt agaaatttgt ctccaagttt cgcgaacata gttaggtgaa tagttagaac
     2401 aaacaccatt tttacctata ttctctttta atcctttaat agaagaccaa ttaatctgta
     2461 atccaggggc taccactaaa aattcgtaac taacgaccga attatctcct aaaataatgc
     2521 tattgttttc aggttgaaag cttgaaacag cattttttat ccattgaaca ccttcaggca
     2581 taacactttt catatcttta cgagaacttt ttaaactcga taccccagca ccaaccaaag
     2641 tccataacgg ttgataataa tgatattcag ccgggtctat aattgctatt tttcctttta
     2701 agctttgatt ttttcttaat agtcttgatg ccacggtaat acctgctgta ccgccaccta
     2761 caataacaat ttgataatac ttactcattt ttccacaccc ttaaattatt ttaatgattc
     2821 tggaaaatct tgatagcctt ctctaatatt cacgacattt tcaaatcctt tattttctaa
     2881 gatacccact gcgattgaac ttctaacacc tgactgacaa tgtacgtata ttttatcctc
     2941 tttattaaat ggaatatttt catttaataa tttaccatgc ggaatattga ctgcttgatc
     3001 taagtgccca ttattccact cttcttcatt acgtacgtcg agtacatttg cttcttcacc
     3061 agtaatatca gcgctgtgga tggattgtgt tagaatttct gattttggta aacgatagcc
     3121 tgctacatta tcaaaaccaa ttaattgtaa agtatgcgtt gcttgttcaa ctgtagattt
     3181 atctccgatt aaatctacat ctttttcaaa atctaaatac caaccgatct ggttaataaa
     3241 gtttttgtta taaggaatat tgattgttcc ttgtgtatgt ccaccgtgaa aggcttcttt
     3301 gctacgaaga tcaaatgcta ttctttcatt atctaaacta ggataaacgt tgtatggttg
     3361 atacattttc ataccaaact gattaatttt tttcatctgt gcaaagtgat ggggtggcgc
     3421 tggttgattt gatgttaatg tttcaacaaa tttagattca tctgttacat taaatgccca
     3481 gttattaatt ttttcgtaac ctagcgtaga cattggtatg gcacctaatg ctttaccaca
     3541 aggacttcca gcaccgtgtc caggccatat ttgaatataa tctggtaaat ttttaacacc
     3601 ttctatagat tggtacatct gttttgcacc aatctctgta gtaccttcca tttgtactga
     3661 tttttctagt aaatcaggtc taccgatatc accaacaaaa ataaagtcac cactaaataa
     3721 acccattgga acacttgagc cgccaccttc atcagtgagt aagaaactga tactctcagg
     3781 ggtatgacca ggtgtgtgca agacttctaa tttaatatta cctacttgaa taatatcttg
     3841 atttttaaca aaatttgtat gttccggcat atttttataa cttaattgat catcaccttc
     3901 gccagataca tatatattgg cgtttaaacg ttttgccaca tcccgaattc cagaagcaaa
     3961 atcagcgtga atatgtgttt cagcagcttg tgtaatttta aaaccttcgc tatctgcaac
     4021 ttccatatat tttgttaaat cacgaacagg atcaattact attgcctctc ccgtacgttg
     4081 acagccaact aaataagatg cttgagataa atgattatcg taaaattgtt tgaaaaacat
     4141 ataaagaact ccttattttg atagatttaa ttaaataaat aaattatgac tcgcgttttc
     4201 agtatcacct atataagtgc ctacaccgcc gtactcaact tcatctctta attcttcttt
     4261 tgtaataccc atgacatcca tgctcatggt acatgcgatt aatttaatat cttgagctat
     4321 cgcttgatta atgagtgatg gtaatgaatc aacgtttttc tttttcatta cgtatcgcat
     4381 catgatatta cctaaaccga acatattcat tttagatagt ggcattttca aaggtgtttt
     4441 tggcaacatc aaatcaaaca tttttgcgat accttgcttt ttaacgttaa ctgtttgcgc
     4501 ttttttcaac gcgttaagtc cccaaaaagt aaagaagata gttacatctc ttcctgcagc
     4561 tctagcgcca tttgcaataa tcattgctgc cactgcttta tctaactctc cactaaatag
     4621 tacaattgtc gtacctttag cagtatgatt cacttgtaag tcttttggtt tttctttttg
     4681 aatgatagca ttaattccat tattactttc atcaagttta actaaagtat gaccagtttg
     4741 tttgacccag cttttaatgt cgctaaggaa tccaggatct gtgactgtaa cttcgatttg
     4801 ctcgcctact tcaatatttt tcacttcttt actgatgttt acaattggac ctggacattg
     4861 aagaccacta tagttaaatt gtttacgctc tgctttaact tctacattag tattttcttc
     4921 gaatgtatta acatcgtcgt tttttgcttc atacgcttta tagccaccgt ctaaattaac
     4981 aatgtcgtag ccttgtttag ctaaatattc gctcgcttta gcacttctgg taccactttt
     5041 acagtgtaca aaataggttg tgtttttatc tttattgaac gattcaatct tatctacagg
     5101 atgtaatgtc gcaccattta tatgtgcaag ctcatattct tcttgtgttc taacgtcaat
     5161 cagttgacct tgtgcaccca atttttctaa ttcttgttta ctgaaattat tgatatgatt
     5221 ttcgttatat tgtttcatat taacccccta ataaatacct atacgggtat attaactaat
     5281 tatttatatt atgtcaaata cggtatgggg tatttgacat gtttactttt atcgtttatt
     5341 atgactttat tcaattaaaa gtggaggaaa taactatgaa ttatgataaa aaaatgatta
     5401 atcgtattaa tagaatacaa ggacaactca atggcgttat taaaatgatg gaagaagaaa
     5461 aagattgtaa agatgtcatt actcaaatca gtgcatcaaa aagttcaatc caacgtttga
     5521 tgggaattat cattagtgaa aatttaatag aatgtgtaaa agcagcagag gataatggtg
     5581 aaaattctga agaattgatt aatgaagccg ttaacttatt ggtaaaaagt aaataatgga
     5641 tatttcaact attattatga tgttacttat tggggtattc ggaggcttta tttcgggtct
     5701 agtaggtata ggtggcgcca tcattattta tccagctatt ttattattac caccactatt
     5761 tggcgctcct gcgtacagtg catacatggc atcaggactt acttccagtc aagtcttttt
     5821 cagtacttta agtgggtcat taaaagcgcg aaaaaaacca gaattttcgc cactattagt
     5881 gctttacatg ggtagtggca tgattatagg cagcatgtta ggtggactgt tagcaaattt
     5941 atttaatgct acatttgtaa atacgatata tattatcatt gctttactcg cattaatttt
     6001 aatgtttatt aacataaaac cgagtttaga tcaatcttcc tttaacaagc cattattagt
     6061 tatggttggt cttttcgttg gtatcatttc aggtatagtt ggagcaggcg gcgcttttat
     6121 tatcattcct atattgctag ttttattcaa attaccaatg aatacggtag ttgctaatag
     6181 tatagtaatt gcttttatat cttcaattgg cgcctttatc attaaactta tacaaggtta
     6241 cattccttta gaagatgcgt tatttttaat aatagggagt attattttcg ctccgttagg
     6301 tttaaaatta ggtaaaaagg tccctagttt tgtacaaaag tggattatca gtattttaat
     6361 tattattgca attattcaaa ttgttttata aataagaaag agaagtacaa ctaatattag
     6421 ttgtacttct ctttcttatt tataaagtta aaacattctc atatagcaag taaaataaca
     6481 tcttaatatt aagttaagag gttttgtgtc aaaaacgatc gtttttaata aagttcaccc
     6541 aagtcatatg gtagttccct ttcactttcc actcattttt gtgaaaagca gtacttggga
     6601 cacaatggca agtataggca attcaatcaa aggcccgatg accagagtcg aagcaatgag
     6661 aggttgattt ggaaaagcgg tgacagctat agccaaggca actggagaat ttcgtgcaat
     6721 aatcgtcaaa cttaaactga ccgtatcttc ataggaaaat ttcagtagtc ttcccactaa
     6781 acggcctaac acatagttga taataaaaaa tatcaaaata ggaataatca agatataaat
     6841 gacttccaaa tggtcaagca agtatgagcc ttgggaagca aacattgcca taatcgcaaa
     6901 tgatagaaag aaaatctgag cattgctgaa aaagggtatg acgatattgc ttaaaactgg
     6961 ttcttttctt ctcaatagaa atcgtgtaag gtgagcaagc acgaaaggaa agatcagtac
     7021 aatcaatata ctctcaatga gtgttgggag aggaatggtt tcaatgctgc cagcgaataa
     7081 tagtaggtat ataggtaaaa gtacgacttg caggatcaaa ttgatcggta gaatcgatgt
     7141 tgaaagagac acattccctt ttgctatgcc tgtaaatgcg aggtaccaat ccgtgcaagg
     7201 tgtcaccata agcataataa aaccaatcca taaagctgga tgatctgata agaaaatagc
     7261 ccccaacccc caggctaata ctggtgtcca tataaaatta atgatagtac tggagccaag
     7321 gaatctgata tttaaaaatg cctttttcaa ttgatgcagt ggtatcgtca aaaacaatcc
     7381 atagagcatt aagaataaaa acgggacgac cagtgtctct gcatatcgtt caattacacc
     7441 tatttgtcct aataagatac ctgaaccaac agcgagcagt ataatcaagg tttgaaactt
     7501 ttctaatatt ccgataaacg cacctctttt ccatttgaaa ttttttataa gatgaacctt
     7561 tctgcaaacc agtataagat agtctatcaa tgattgtctt gagtgccaat tgaagtaata
     7621 gaaaaaagat aaatttggtg gtactcatca ttacgaatat attgacaata gtgctaaaca
     7681 tttttaatat atgagtatat ataaatataa ccatatttat attaagagct accattatga
     7741 ttagtaagag gtgagccatc ataaaagata ttaacgaaaa caaaattgtt ggtaataaaa
     7801 aaggtcttga tgaagaaact ttatttgttg tatcccaaac tttcaaagca ttaagtgatc
     7861 caacacgaat cagaatactg aatttgctgt ttcttaaaga atattccgta aatgagattg
     7921 ctgaaaacct acatttaaga caatccaccg tttcgcatca attacgcttt ttgaaaaatt
     7981 tgcgcttggt caagtataga agggaaggta cgactctata ttattcccat gatgatgccc
     8041 atgtaatgaa catgttgaaa gaaacaatca accatgctcg tcaccattga ccaaaaaatt
     8101 atataaaccc atctattata cccatctata aatattaata ggagatgatt gaatgagtca
     8161 tgaacataac catacacatg gtgcgaacaa aaaaaccttg atgatcagtt tcataatcat
     8221 cacaggatat atgattatcg aagcgattgg gggaattttg accaatagcc tcgccttact
     8281 agctgatgcg ggccatatgt taagtgattc aatttcccta ggtgtcggtt tgttggcttt
     8341 tatacttgga gaaaaagcgg ccgattatag taaaacatac ggatataaac gatttgaaat
     8401 tcttgcagct gtatttaacg gagttactct ggtactgatt gccatctaca ttttctatga
     8461 ggcctatcaa cgttttacag atccacctga agtcgcctct acgggtatgt taatcatcgc
     8521 aactattgga ctactggtaa atattcttgt agcatggatt ctcatgcgag gcggggatac
     8581 gaaggaaaac ttgaatatac gtgcagcatt tttacatgtg ataggcgatc tgcttggatc
     8641 ggtgggggcc attattgcgg ctctgatgat cctcttcttc aactggggct gggcagatcc
     8701 gttggccagt gtgattgttg cagtgcttgt attgataagt gggtggcgtg tgacaaaaga
     8761 tgccgttcat gtattgatgg agggtacccc taaaaacgtt gaaattgacg cagtgattaa
     8821 aaccatagag agcattccag gagtaaataa tatgcatgat ctacatgtat ggagtattac
     8881 aagtggccaa aatgcacttt catgccatgt ggtggtagat ggtaccattt ctattcagga
     8941 aagccaggaa attctgagaa caattgaaga cgagttggaa gatgagaata ttggacatgc
     9001 aacgattcag atggagagtg aaaaccaccc tcataaggat tctgtaatgt gcagggatga
     9061 tgatattacg catagtcatg aacactaaaa aatagaatat accactataa tgatcaaaaa
     9121 tgttagcaag ataacgattt tgacctataa attgaagatt tttaatgtat acgattttat
     9181 taactgttca tagtattaga tagaaagaaa aaattcgagc ttctgaatat cgtgaatcga
     9241 ttaatctgca ttctcgttgg gcggttaggt ctagtgatct cctacaagcc attaatgagg
     9301 tgaaacctca tattatccat tttagtgggc atgggtcatc tgaacatgac atcgttttag
     9361 aaactgcaga aggcaaaccg agttttttat ctaaagaaaa agtgaccctt ttaatgaaaa
     9421 ccatgtcaga gtccatcaaa ttagttatct ttaataattg cttttccaac ggccaagccg
     9481 aaatggtgac agagcatgtt ggttttgcta ttggaatgaa tgaagcaatt caggacgaag
     9541 cagccaaaga atttgctgca cagttttatt ctgccctagg atttggtcat actgtccaaa
     9601 aagcatttga acaaggcaaa ttagcgctta gcttagaagg aattgaaggg gatgaaatac
     9661 ctgaactcta ttcgagagaa gggttagatc ctaatgagca tatattggta aaaccggact
     9721 tttaataacc acttagtaaa gtaaaaaaag ttactttaca tgccctcacc gattggtagt
     9781 tactaaaata caaggtatgg ctgatgtgaa tagattagta tcagccatac ccttttataa
     9841 gaagagactt tataccatca atattccttt acgtaacaat tcgaaaatct tgtccaattt
     9901 cgggcatatg tctatctctc caaatcagtg cagctatttt cttgtaaact ttgattttta
     9961 aaaggaaagt gtattaatcc attggtatct tgacgatttt atatttgaaa gaccctgaat
    10021 cgagctaaaa agttggtaat agacaaaagg taaaactaac aagaaagtaa aaggtttaat
    10081 tggataattt aaagaaaaaa tgttataatc acattaacaa gtagaatgtt atgcatgaaa
    10141 caccgaaccc ccaatctatg tcagtagatt gggggtttta tatggtgtat tggtttagtc
    10201 atctagtcat ttatcacgac tgtccaacca ataagcgaac aatgtgatga ttacaccaac
    10261 aataatgggt gcaatcaaca attcaaatag aatgctatgc atgatgtcac ctccttccat
    10321 gctatcagca cagaagttaa ctagatgacc gttataattc tatcacatca cctaatttaa
    10381 aagaacacaa tgtcttatca gataagtcaa cttaactcga ctatacagtt gctccctcga
    10441 ttacaaattt ataaattcgc aaaagaaata aattactagg ggaagacgtt tatcacttta
    10501 aaagatggtt tacatcagtt taaagcttta ttttgcatag gattttaaga gaccagtaac
    10561 ctctgcatta aggttactgg tcttataatt ttcctccata ctttaaaaac ttcttctctt
    10621 ctggttttag atgatattta atgtgtaagt ctattatcaa ttcttacttt catttcattt
    10681 attacttcta aatcaagatc atctattagc tcaaatacca tttctaactt cctattttca
    10741 tcaatagtaa gtaaatctaa tatgtccatt aggctctctt cttcttcatt ttcttcgtca
    10801 tcaaaacctt tctgatgata gttatttata tcttcttgat aacttttatg accaaatttt
    10861 tctctattac tatgatacaa gtttccaagt aattctcggt tctgttgcaa agttgaattt
    10921 ataatataat tttaacaaaa aggagtcttc tgtatgaact atttcagata taaacaattt
    10981 aacaaggatg ttatcactgt agccgttggc tactatctaa gatatgcatt gagctatcgt
    11041 gata
//
