LOCUS       CP027494                2761 bp    DNA     linear   BCT 19-MAR-2018
DEFINITION  Staphylococcus aureus strain ST2594 isolate 004 plasmid unnamed9.
ACCESSION   CP027494
VERSION     CP027494.1
DBLINK      BioProject: PRJNA436877
            BioSample: SAMN08634544
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Bastos,L.R., Martins,M.C.F., Albano,R.M., Marques,E.A. and Leao,R.S.
  TITLE     Whole genome sequencing of ST2594 MRSA strain causing skin
            colonization and low-grade infection in surgical site. Implications
            for MRSA pathogenesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Bastos,L.R., Martins,M.C.F., Albano,R.M., Marques,E.A. and Leao,R.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2018) Departamento de Microbiologia, Imunologia e
            Parasitologia, Universidade do Estado do Rio de Janeiro, Av. 28 de
            Setembro, 87, Vila Isabel, Rio de Janeiro, RJ 20551-030, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.5; MAUVE v. 2.4.0
            Assembly Name                     :: Saureus141ST2594
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: BA000018.3
            Genome Coverage                   :: 40.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/06/2018 13:58:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.4
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,922
            CDS (total)                       :: 2,883
            Genes (coding)                    :: 2,764
            CDS (coding)                      :: 2,764
            Genes (RNA)                       :: 39
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 3 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 65 of 119
            Pseudo Genes (incomplete)         :: 37 of 119
            Pseudo Genes (internal stop)      :: 29 of 119
            Pseudo Genes (multiple problems)  :: 12 of 119
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Lab2_UERJ_19239.
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ST2594"
                     /isolate="004"
                     /isolation_source="Femural surgical wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed9"
                     /geo_loc_name="Brazil: Rio de Janeiro"
                     /lat_lon="22.8951 S 43.2431 W"
                     /collection_date="2015-03-11"
                     /collected_by="Leonardo Bastos"
     gene            complement(885..1208)
                     /locus_tag="C6V85_14415"
     CDS             complement(885..1208)
                     /locus_tag="C6V85_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacJ"
                     /protein_id="AVO70340.1"
                     /translation="MPYLYLAIAIITEIIGTSFLKSAEGFTKLWPTLGTLISFGICFYF
                     LSVTMKYLPLNISYATWAGLGLVLTTIVSVVIFKESVNLISIFSIILIIIGVVLLNVFG
                     SSH"
                     /name="quaternary ammonium compound efflux SMR transporter
                     QacJ"
     gene            1533..2531
                     /locus_tag="C6V85_14420"
     CDS             1533..2531
                     /locus_tag="C6V85_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="AVO70341.1"
                     /translation="MQYNTTKYIDENQGNETLKDMSKSGKQRPWREKKIDNVNYADILE
                     ILKIKKAFNVKQCGNVLEFKPTDEGYLKLYKTWFCKSKLCPVCNWRRSMKNSYQAQKVI
                     EEVVKEKPKARWLFLTLSTKNAIDGETLEQSLKHLTKAFDRLSRYKKVKQNLIGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQDEWINLWQKALQVDYKPVANIKAI
                     KPNKKGDKDIQSAIKETSKYSVKSSDYLTGNHEKDSEIVKDLEQGLYRKRMLSYGGLLK
                     QKHKILNLDDAEDGNLIQTSDDEKATEEEQKAHSITAIWNFEKQNYFLKNL"
                     /name="protein rep"
     ARG             complement(889..1208)
                     /name="qacJ"
                     /gene="qacJ"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacJ"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="97.2"
                     /coverage="100.0"
     Typing          1534..2531
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.69"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtttttatgc taatttttaa atttgcatgt aactgggcag tgtctaaaaa aatagccact
       61 ggttttgctc tgattttgtg ttgtgaaatt aaaatatatt tatttggctc tcatttgctt
      121 tttaagagct tataaaccct tttaggtata aaactatata aataacccct atttctttaa
      181 aataccccct taaaattcaa aataaaggta tttaaattta gacaaataaa aaagccaatt
      241 cgtataaaaa cgaactgact tttaaaggac tattctttta tcgtggtatt aatttttata
      301 tctgtgccac gacaccgatt cttaagtatg tagtattaat ttttatatct gcgccacaac
      361 gccgattctt ttgtgtttga tataaaaatc gacggaggtc gatttcgatt ttagcacaac
      421 gtttggaaca aacgtataag tgcgccctta cgggaattaa ctagattata acgacgaatt
      481 ttagacctgt aaagcaataa caattgggtc taccaggtag ataaagtgta caaagtagac
      541 ccgaaaaagt tatttatttt gaagattttt cgtagatata atttgctaac aaacttgcta
      601 aaactgaata aagaaaaata gttaaactca tgtaatcact ccattttttc gtttgttcct
      661 aaatggagtg aagttatgaa ctccataatt ctggagtgtc tccatgtcta cgcatagaca
      721 cgcatttatg ccgagaaaat ttattgtgcg ttgagaagaa cccttaacta aacttgtaga
      781 cgaatgtcgg catagcgtga gctattaagc cgaccattcg acaagttttg ggattgttaa
      841 gggttccgag gctcaacgtc aataaagcaa ttggaataaa gcaattaatg acttgatcca
      901 aaaacgttaa gaagtacaac accaataata attaatatga ttgaaaatat actaattaaa
      961 ttgacacttt ctttgaaaat tacaactgaa actattgttg taagaactag tcctaatcct
     1021 gcccaagttg cataagatat attgagtggt aaatatttca tagttacact taaaaaataa
     1081 aaacatatcc cgaatgagat aagtgtccct aatgttggcc aaagttttgt aaatccttct
     1141 gctgatttta agaaactagt tcctattatt tcagttataa ttgctattgc taaatataag
     1201 taaggcatta ttatcatcct tttttaaatt ttaataattt aaattttagt ttatattaaa
     1261 ttaaataaaa gccttgtatt tttcttgtga ttcgcgaggt attgattttt gtgatttcaa
     1321 aaatttgaag ggttttgaaa cgagttgaaa acgagtttct tcttgtcttg atactatata
     1381 gaaataactc gattttataa aaacgcttat tatccttgtt atgaaagggt ttgtagcgtt
     1441 ttttctgcgt gaaaagaatt tttgacataa aaaaacccca gtagtatgat taaagcgtct
     1501 actcaataaa caactactgg aggtctttct atatgcaata taatactaca aaatatatag
     1561 atgaaaatca aggtaatgaa acattaaaag atatgtctaa aagtgggaaa caacgcccat
     1621 ggagagagaa gaaaatcgat aatgtaaact atgcagatat actggaaatt ttaaagataa
     1681 aaaaggcttt taacgtaaaa caatgtggta acgtcttaga atttaagccg actgatgaag
     1741 gatatttaaa gctatataaa acgtggtttt gtaagtcaaa actatgtccg gtttgtaatt
     1801 ggaggcgttc aatgaaaaac agttatcaag cccaaaaagt aattgaagaa gttgttaaag
     1861 aaaaaccaaa agcacgttgg ttatttttaa cactttcaac aaaaaacgcg attgatggcg
     1921 aaactttaga acaaagttta aaacatttaa ctaaagcttt tgatagattg agtagatata
     1981 aaaaagtaaa acaaaatttg attggtttta tgcgttcaac tgaggtaaca gttaataaaa
     2041 atgatggtag ttataaccaa catatgcacg ttttattgtg tgttgaaaat gcttatttta
     2101 gaaaaaaaga gaattatata actcaagacg agtggatcaa tttatggcaa aaagcacttc
     2161 aagttgatta caaaccagtg gcaaatatta aagcgattaa gccgaataaa aaaggtgata
     2221 aagatataca atcagcaatc aaagaaacat caaaatactc ggttaagtca tcggattacc
     2281 taacaggaaa ccacgaaaaa gattccgaaa tcgttaaaga tttagagcaa ggtttataca
     2341 gaaaacgcat gttaagttat ggtggtttgc ttaaacaaaa acataaaatt ctaaatctag
     2401 atgacgctga agacggcaat ttaattcaaa caagtgatga tgaaaaagcg actgaagaag
     2461 aacaaaaagc acattcaatc acggcaatat ggaatttcga aaaacaaaat tatttcttaa
     2521 aaaatttgta acgttagctt atcaaagcta gcgttttttt atgtatttta gcccctgcag
     2581 gaactatagt gttcaaccgg catggttgcc cagtttttat gctaattttt aaatttgcat
     2641 gtaactgggc agtgtctaaa aaaatagcca ctggttttgc tctgattttg tgttgtgaaa
     2701 ttaaaatata tttatttggc tctcatttgc tttttaagag cttataaacc cttttaggta
     2761 t
//
