LOCUS       CP027493                2896 bp    DNA     linear   BCT 19-MAR-2018
DEFINITION  Staphylococcus aureus strain ST2594 isolate 004 plasmid unnamed8.
ACCESSION   CP027493
VERSION     CP027493.1
DBLINK      BioProject: PRJNA436877
            BioSample: SAMN08634544
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Bastos,L.R., Martins,M.C.F., Albano,R.M., Marques,E.A. and Leao,R.S.
  TITLE     Whole genome sequencing of ST2594 MRSA strain causing skin
            colonization and low-grade infection in surgical site. Implications
            for MRSA pathogenesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Bastos,L.R., Martins,M.C.F., Albano,R.M., Marques,E.A. and Leao,R.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2018) Departamento de Microbiologia, Imunologia e
            Parasitologia, Universidade do Estado do Rio de Janeiro, Av. 28 de
            Setembro, 87, Vila Isabel, Rio de Janeiro, RJ 20551-030, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.5; MAUVE v. 2.4.0
            Assembly Name                     :: Saureus141ST2594
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: BA000018.3
            Genome Coverage                   :: 40.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/06/2018 13:58:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.4
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,922
            CDS (total)                       :: 2,883
            Genes (coding)                    :: 2,764
            CDS (coding)                      :: 2,764
            Genes (RNA)                       :: 39
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 3 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 65 of 119
            Pseudo Genes (incomplete)         :: 37 of 119
            Pseudo Genes (internal stop)      :: 29 of 119
            Pseudo Genes (multiple problems)  :: 12 of 119
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Lab2_UERJ_19239.
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ST2594"
                     /isolate="004"
                     /isolation_source="Femural surgical wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed8"
                     /geo_loc_name="Brazil: Rio de Janeiro"
                     /lat_lon="22.8951 S 43.2431 W"
                     /collection_date="2015-03-11"
                     /collected_by="Leonardo Bastos"
     gene            complement(<1..86)
                     /locus_tag="C6V85_14395"
     CDS             complement(<1..86)
                     /locus_tag="C6V85_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="AVO70336.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYR"
                     /name="IS6 family transposase"
     gene            complement(201..515)
                     /locus_tag="C6V85_14400"
     CDS             complement(201..515)
                     /locus_tag="C6V85_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="AVO70337.1"
                     /translation="MKSSSTSPLTNDSIQEVSKIFKMISDPTRLSILFLLQKEELSVGV
                     IAQSLSMEQSAISHQLKTLKTSRLVKSKRVGKNMIYSLDDLHIFSILEQVSTHIGEQEK
                     "
                     /name="transcriptional regulator"
     gene            760..2688
                     /locus_tag="C6V85_14405"
     CDS             760..2688
                     /locus_tag="C6V85_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_763617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="AVO70338.1"
                     /translation="MIEMKEIQEQHSHENNSHDHDHDHGKMPIILYFIGLALALIAFFL
                     SKEYQILQNILFLIATISAGYHVIVLEGLGETIHNSKSQKRFIPNSHILMGLAAIGASL
                     MGNFGEGALLIFIFSGAHFLEDYAEGRSRREITKLLEMNPTTARLILPDGSTKNVKVNE
                     LKVGNHLQVLNGDQVPIDGIILSGSTSIDESSINGESIPKEKSKGDEVFGSTINGTGTF
                     TMEVTKENKDTXFSKILQLVNQNQDNQTKAASIIQKFEPKYVTFVLVAILLFILLTPFL
                     LDWTWSQSFYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYLSQLADIQ
                     AIAFDKTGTLTKGKPEVTNHYFTDSMDEEEIIDIVVALEKNSNHPLADAILRKFEPKNQ
                     LSIEVENQIGKGLSGDYNGRKYRIGKPTSFDDVSDEYVRLNNQWASEGKTVVYLAVDEE
                     VIGLIALMDVPSKYAKETIEYFNKQGIHTTLITGDSEMTGKAVASKLGIDEVIANVMPD
                     EKSKIIDKQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEVSDLVLMQNNLT
                     KLTQSHKISTKMNRLIWQNIIFSMAVVAFLIIVSLVGLTDIAISVIVHEGSTLVVILNG
                     LRLLNSK"
                     /name="heavy metal translocating P-type ATPase"
     gene            2811..>2896
                     /locus_tag="C6V85_14410"
     CDS             2811..>2896
                     /locus_tag="C6V85_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="AVO70339.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYR"
                     /name="IS6 family transposase"
ORIGIN
        1 cgataactca atgcatatct tagatagtag ccaacggcta cagtgataac atccttgtta
       61 aattgtttat atctgaaata gttcatacag aagactcctg actccttttt gttaaaatta
      121 tactataaat tcaactttgc aacagaaccc tttatttgaa ctaatgaatt gtaaaataat
      181 tttaactttg tagtttgtgc ttatttttct tgttctccga tatgagttga aacttgctca
      241 agaatactaa aaatatgaag atcgtcaagg ctataaatca tatttttacc aactcttttt
      301 gattttacta atcttgaagt ctttaaagtt tttaactggt gagaaatggc tgattgttcc
      361 atacttaaag actgcgcaat tactccaaca ctaagttctt ctttctgtaa aagaaataaa
      421 attgaaagac gtgtagggtc actaatcatt ttaaatatct tacttacctc ttggatagaa
      481 tcatttgtta acggagaagt tgatgatgat ttcataaaac ttcctctctt tcatatgtgt
      541 gttcgttcat gtttaggtta gcacaagatt tttataaagc aaggtttttc cataagcaga
      601 gctgttttat agtccaattt ttttattaac taaataagat aactattttt ttgacaaatc
      661 agatagagca atatataatg aacgcataaa catatgaatg ttatttcata tgtaattcgt
      721 aagcgttacg atttcaaaaa aatagtttta ttaagaaggg tgatagaaat gaaagagatt
      781 caagagcaac actcacacga aaataatagt cacgatcatg accatgatca tgggaaaatg
      841 ccaattattt tgtactttat tggtttagca ctagcattga tagctttctt tttgagtaaa
      901 gagtatcaaa tcttacaaaa tattttgttc ttaatcgcta ctataagcgc tgggtaccat
      961 gttattgtcc ttgaaggact tggggagacg atccataact caaaaagtca aaaaagattt
     1021 atacctaatt ctcatatctt aatgggatta gcagcaattg gagcatcttt gatgggaaat
     1081 tttggagaag gtgcgttatt aatctttatt ttttctgggg cacattttct agaagattat
     1141 gcagaaggaa gaagtagacg agaaattaca aaattactcg aaatgaatcc cacaactgct
     1201 cgtttaattc tcccagacgg tagcacaaaa aatgtgaaag ttaatgaatt aaaagttgga
     1261 aatcaccttc aagtattaaa tggtgatcaa gtccctattg atggaattat tttgtctgga
     1321 tctacgtcta ttgatgagtc ttctattaat ggagagagta taccaaaaga aaagtccaag
     1381 ggagacgaag tttttgggag cacaataaat ggaacgggca cttttacaat ggaagtcacg
     1441 aaagaaaata aagatacggk gttttcaaaa attttacaat tagtgaatca aaaccaggat
     1501 aaccaaacta aagctgcaag tattattcaa aaatttgaac caaaatatgt aacatttgtt
     1561 ttagttgcca tattactatt tatattatta actccatttc tacttgattg gacatggtca
     1621 caaagctttt atagaggatt ggttctttta gttgcagctt cgccatgtgc tttggcagca
     1681 gctactgtat cggcaacttt atcaacaact tctaatttag ctaaaaaagg cgttctttct
     1741 aaggggagtt cttatctatc tcaattagct gatattcaag ctattgcatt tgataagacg
     1801 ggaactttaa ccaaaggaaa acctgaagtc acgaatcatt attttactga ttctatggat
     1861 gaagaagaaa taattgatat tgtagtagct cttgagaaaa attccaatca tcctttagcg
     1921 gatgctattt taagaaaatt tgaaccaaaa aaccaacttt ctattgaagt agaaaatcag
     1981 attggaaaag gattgtccgg agactataat ggtcgaaaat atcgtatagg taaaccaact
     2041 tcttttgacg acgtctcaga tgaatatgtt cgattaaata atcaatgggc atctgaaggg
     2101 aaaactgtcg tatatctagc agtagatgaa gaagttatag gccttattgc acttatggat
     2161 gtaccaagta aatatgctaa agaaactatt gagtacttca ataaacaagg tattcatact
     2221 acgttaatta caggtgattc ggaaatgaca ggaaaagcag tcgcaagcaa gctaggtata
     2281 gacgaagtga ttgccaatgt catgccagat gaaaaatcta aaattattga caaacaaaaa
     2341 gaaaaatatg gtgtgaccgc aatggtagga gatggtgtaa atgatgctcc agctcttgtc
     2401 aatgcagatg taggaatagc catgggagat ggaactgatg tggcagtaga ggtttctgac
     2461 ttagtattaa tgcaaaataa cttaacgaaa ttaacacagt ctcataaaat ttctacgaaa
     2521 atgaatcgtt taatttggca gaatattatt ttttcaatgg cagttgtagc ttttctaatt
     2581 atcgtgagct tagtaggatt aacggatata gccattagtg tgatcgttca tgaaggaagt
     2641 acattagtcg taatattaaa tggtctccga ttattaaatt ctaaatgaat acatgaaatt
     2701 ggataaaatc gtgtatcgag aatcctatac ctatagggtt cttttttggt tctgttgcaa
     2761 agttgaattt atagtataat tttaacaaaa aggagtcagg agtcttctgt atgaactatt
     2821 tcagatataa acaatttaac aaggatgtta tcactgtagc cgttggctac tatctaagat
     2881 atgcattgag ttatcg
//
