LOCUS       CP027490               10762 bp    DNA     linear   BCT 19-MAR-2018
DEFINITION  Staphylococcus aureus strain ST2594 isolate 004 plasmid unnamed5.
ACCESSION   CP027490
VERSION     CP027490.1
DBLINK      BioProject: PRJNA436877
            BioSample: SAMN08634544
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10762)
  AUTHORS   Bastos,L.R., Martins,M.C.F., Albano,R.M., Marques,E.A. and Leao,R.S.
  TITLE     Whole genome sequencing of ST2594 MRSA strain causing skin
            colonization and low-grade infection in surgical site. Implications
            for MRSA pathogenesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10762)
  AUTHORS   Bastos,L.R., Martins,M.C.F., Albano,R.M., Marques,E.A. and Leao,R.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2018) Departamento de Microbiologia, Imunologia e
            Parasitologia, Universidade do Estado do Rio de Janeiro, Av. 28 de
            Setembro, 87, Vila Isabel, Rio de Janeiro, RJ 20551-030, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.5; MAUVE v. 2.4.0
            Assembly Name                     :: Saureus141ST2594
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: BA000018.3
            Genome Coverage                   :: 40.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/06/2018 13:58:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.4
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,922
            CDS (total)                       :: 2,883
            Genes (coding)                    :: 2,764
            CDS (coding)                      :: 2,764
            Genes (RNA)                       :: 39
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 3 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 65 of 119
            Pseudo Genes (incomplete)         :: 37 of 119
            Pseudo Genes (internal stop)      :: 29 of 119
            Pseudo Genes (multiple problems)  :: 12 of 119
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Lab2_UERJ_19239.
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10762
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ST2594"
                     /isolate="004"
                     /isolation_source="Femural surgical wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed5"
                     /geo_loc_name="Brazil: Rio de Janeiro"
                     /lat_lon="22.8951 S 43.2431 W"
                     /collection_date="2015-03-11"
                     /collected_by="Leonardo Bastos"
     gene            <1..90
                     /locus_tag="C6V85_14270"
     CDS             <1..90
                     /locus_tag="C6V85_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="AVO70314.1"
                     /translation="IYALYKKNRRSLQIYGFSPCHEISIMLAS"
                     /name="IS6 family transposase"
     gene            complement(132..1058)
                     /locus_tag="C6V85_14275"
     CDS             complement(132..1058)
                     /locus_tag="C6V85_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AVO70315.1"
                     /translation="MTIRSFGWVQNPSDFKKLKQTVEIFSKNSKQYQLLKESLIKEKLH
                     NFKDIQSSLQEKLNDGIEEFSYIELVGKNLGKDRKTPKSRKYSLPNALIQISLDSQSAS
                     TKNKHFTDEWSSDGFLRWAVSLNFVEQNRQTDIFKITSRGKQFIETQNEVEMNKVLREA
                     FLSYPPATRVLEILNNNTEVTKFFIGNRLGFKDEPGFTSYSEELMLDWIKESENKKEEL
                     KIRSDVEGTADKYARMICGWLKKVGFVSQKSIQYNSLLEKNRKITGFSLYSITAKGSHA
                     LRQAQGNSSNSKREKFVMWEFLGSVAK"
                     /name="hypothetical protein"
     gene            complement(1395..2162)
                     /locus_tag="C6V85_14280"
     CDS             complement(1395..2162)
                     /locus_tag="C6V85_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_863231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="AVO70316.1"
                     /translation="MRTIKMVADELGVTKQTIVNNAKSLNITFEKENGINYIDDNDYLK
                     IIEKITKKESTIQNKESVKKELFGENTAKAEKNIYNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKXIENLTKQLDQQNSNVNTLNKLLENQQILALESNKKIQKLESQLEEERQLN
                     YSFDTATNNRQNINPQEATFTEESQNINQQQTKRQPTEVNYKDIAEEKKNDSTDEGELT
                     KEDKFVEETKSDPDDNREVEPPKKGFWSRFFGN"
                     /name="DUF536 domain-containing protein"
     gene            2602..3537
                     /locus_tag="C6V85_14285"
     CDS             2602..3537
                     /locus_tag="C6V85_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_863230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="AVO70317.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSIQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELISFNLLTQERVGLN
                     KPNRLYLLKPNYDIKASHSKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTENNDTD
                     YIKTESNDTNDLNDNKLTFPSNHTNHSNHDNSNFNNEALKFQLLEELPQSIKNYLSNFE
                     VAEIKIIKTVLLKAKTSFNNAIDSYYLLEDMEIEILHVLKRFKAMLIQKNETVESMQGY
                     LMKSLKSEFAEMHTLNKRRDHLPITSLFNQ"
                     /name="replication protein"
     gene            complement(4590..4818)
                     /locus_tag="C6V85_14290"
                     /pseudo=""
     CDS             complement(4590..4818)
                     /locus_tag="C6V85_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505090.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            5232..5357
                     /locus_tag="C6V85_14295"
     CDS             5232..5357
                     /locus_tag="C6V85_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="AVO70318.1"
                     /translation="MTVYFYIWXKKEYLASEIFDLTESEKQEINHQMIDEIEEEK"
                     /name="Rep protein"
     gene            5467..5652
                     /locus_tag="C6V85_14300"
     CDS             5467..5652
                     /locus_tag="C6V85_14300"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AVO70319.1"
                     /translation="MGTTQYLVGTFWVQNKKMYTFLKSLYPLKCLASPDLPFKMVANFP
                     YALTKNLEPIQNSXVF"
                     /name="hypothetical protein"
     gene            6333..6773
                     /locus_tag="C6V85_14305"
     CDS             6333..6773
                     /locus_tag="C6V85_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="AVO70320.1"
                     /translation="MNIFVKNTKDLKIDDLLNILRERIRVFVIEQNCVYEEIDKNDNNA
                     IHVVFYDQDKIIAYSRIIEHETFYSFGRVLVVNKYRKKGLGKKVVSRTLREIKKLQNEN
                     SIKKPIKIQAQAYLENFYKTFGFVSKSDIYLEDDIPHLDMEK"
                     /name="GNAT family N-acetyltransferase"
     gene            7459..7666
                     /locus_tag="C6V85_14310"
                     /pseudo=""
     CDS             7459..7666
                     /locus_tag="C6V85_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691021.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(7803..7952)
                     /locus_tag="C6V85_14315"
     CDS             complement(7803..7952)
                     /locus_tag="C6V85_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="AVO70321.1"
                     /translation="MKTLNEKQLKDINGGKGIVGEVTGEVTSIGNAISNGWHDLKQSVA
                     DTTK"
                     /name="bacteriocin"
     gene            complement(7973..8116)
                     /locus_tag="C6V85_14320"
     CDS             complement(7973..8116)
                     /locus_tag="C6V85_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-type signal sequence"
                     /protein_id="AVO70322.1"
                     /translation="METLNEKQLKDINGGRETGCSGDFVEDGIMCNIGQGIGKAINKVR
                     GK"
                     /name="bacteriocin-type signal sequence"
     gene            complement(8273..10429)
                     /locus_tag="C6V85_14325"
     CDS             complement(8273..10429)
                     /locus_tag="C6V85_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="AVO70323.1"
                     /translation="MAIKFIRQYDEKDCGPTCLAMISQFYGKRVSIPRLREYAKTDKLG
                     TNLYGLVQAGKKIGIELTGVKADSFDDLKQAQLPVMVHIINQQGYDHYIIIEKIKNNTL
                     YIVDPAKGKYKLSSTEFGKYWTNIAVLIDKTDDFTNDNESPSYLNIFIDIFKNNYGKLL
                     FIAFISLFINIVGIVGALYFKLLTDHIIPSNVLKNLHIISFGILLLYIINALINYLRFQ
                     LILHLSLKIDVNLMKDYFYHVLHLPMNFFDTRKSGEILQRFMDTSKIREALSSSTVTLL
                     VDTFMIIIGAILLYMQSPLLLLITIIFIPCFIICSYTLRKPFEKYNQKVAEKDAELSSY
                     LIESFDGSNTIKSYQSEDDRFYIGTTKFNGIIENXLKLGRFSNIQLTVNNFLKLTISLV
                     ILWLGSYLVMTDSMTLGSLLAFNALTIYYLDPIERLINIQPTLQSSFVAARRIAEITDL
                     ETETELYTSKQPYTFKNNIRIENVNFQYGFRSVVLKDINLNIKKGQKVAIVGESGSGKS
                     TIGKLLNRYYTASEGNIMIDNYIIDDIDLSDLRKNIGYVSQNTFLFADTIKNNLLHGSN
                     KYKSDEDIVKACQLAESLDFIQKFPDQFNTMLEKEGANLSGGQGQRLSLARTFLKDPDI
                     YIFDEATSALDSLTENKIMEHIDLLTQHGKTVIIISHKLSTIKNADNIYVLNEGQIAEN
                     GTHDELIHLNGIYKNLWQLQMKID"
                     /name="peptide cleavage/export ABC transporter"
     BGC             3273..10762
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2603..3537
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.893"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atttacgctc tatataaaaa gaaccgcagg tctcttcaga tctacggatt ttcgccatgc
       61 cacgaaatta gcatcatgct agcaagttaa gcgaacactg acatgataaa ttagtggtta
      121 gctatatttt tttactttgc aacagaacct agaaactccc acataacaaa tttttcacgc
      181 ttgctattac tcgaattacc ttgtgcttgc cttagtgcat gactaccttt agcagtaatt
      241 gaatatagtg aaaaaccagt gatttttctg ttcttttcca gcaaagaatt atattgtatt
      301 gatttttggc taacaaagcc aactttcttt aaccagccac aaatcattct cgcatattta
      361 tctgctgtgc cttctacatc tgatcttatt tttaattctt cttttttatt ttcagactct
      421 ttaatccagt ctaacataag ttcttcagag tatgaggtga aaccaggctc gtctttaaaa
      481 cctaaacgat tacctataaa aaactttgtt acttctgtat tattatttaa tatttctaag
      541 actcttgtag caggaggata acttaagaat gcctctctta aaaccttatt catctctact
      601 tcgttttgag tttcaataaa ttgttttcct cttgaagtaa ttttaaatat atctgtctgg
      661 cgattttgct ctacgaaatt caaactaact gcccatctta aaaaaccatc tgaagaccat
      721 tcatcggtaa aatgtttgtt ttttgttgaa gcgctttgtg aatctaaaga aatttgtatt
      781 aatgcgttag gtagtgagta ctttctagat ttcggagttt ttctatcttt accaagattt
      841 tttcctacca attctatata tgaaaactct tcgatgccat cattcaattt ttcttgcaat
      901 gacgattgaa tatctttgaa attatgaagt ttctctttaa ttagactctc tttcagcaat
      961 tggtattgtt tcgaattttt actaaatatt tctactgttt gttttagttt tttaaaatca
     1021 gatggatttt gcacccaacc aaaagatctt atagtcatat atctccctcc tgtctatctc
     1081 actttgattt tacagttatt tgtttgaaca cacaattata tagtaaagaa gaatactgta
     1141 ataaaacagt tacgctatat ctaattaagt agtgttacat tttctcctcc aataagatat
     1201 tatttccatt cttttattct tgttttatat accgatagat ataaattttg gattttaaaa
     1261 gtatcctcct agagagtgct aagataagat attttgagtt taaattaaca taaattatga
     1321 ttatcagaag tttcatcact ataaaaggtg tatcaaagat ttatactccc ttgatacacc
     1381 ttttacattc atttttaatt accgaagaat cgactccaaa atcccttttt aggtggttct
     1441 acctccctat tatcatcagg atcactcttc gtttcttcaa cgaacttatc ctcttttgtt
     1501 aattcacctt catctgtact gtcattcttt ttctcttcgg ctatatcctt gtaatttact
     1561 tctgttggtt gacgctttgt ttgttgttgg ttgatatttt gcgattcttc agtaaaagtt
     1621 gcttcttgcg gattaatatt ttgtctatta ttcgtcgctg tatcaaaaga ataattaagc
     1681 tgtctttctt cttctaattg actttctagt ttttgaattt ttttattgct ttctaaagct
     1741 aatatttgtt gattctccaa caatttattt aatgtattaa cattgctatt ttgttgatct
     1801 aattgcttag ttaaattctc aatwtatttt tcatcatttt tagctctagt ttcaaagatt
     1861 tctatttgct tttctaattc gtttactttt gtttttaatg tctcagaact atttgaatta
     1921 ttatatatat tcttttctgc ttttgcagta ttttcaccaa acaattcttt ttttactgat
     1981 tctttatttt ggattgtact ctctttctta gtaatttttt ctataatttt caaataatca
     2041 ttatcatcaa tataattaat cccattttct ttttcaaaag ttatatttaa acttttagca
     2101 ttattaacaa tagtttgttt agttacacct aattcatcag caaccatttt aatagttctc
     2161 ataacatcca cccttaggta cctaatttag tacctaaatt ttaccaccta atgcagttta
     2221 tttcatcaat taccactaaa tttactacct atttactaca tatgcaccta attaactacc
     2281 tataatatgt ttttgaagct taattaggta ctaaaacaat gaactacaca ttttgccatg
     2341 gcaatgtgct tttaactaca tttaaaactc tttatatact ttcttaaaag tgttaatatt
     2401 gctttataaa tactaaaaga gtcaatgcta ttggcgtagc attgactctc ggtaataaaa
     2461 cgattcgcac tcgtttgttt atatattttt tgatacttgt attatatcta tctaatcatc
     2521 taagtgcaag cacaaaacat ataacttacg taaaaattgt tttattacct caatcctaaa
     2581 aatggaaatg aggtttttat tatgcccaat tttgaaaaat acaatttatc acaagtaaaa
     2641 actgaaaggt tttatcaact acctaaatat ttattcgaag atgcatattt taaaaaaatg
     2701 tcggcggaag ccaaaattat gtatgcgtta ttaaaagatc gctttgaatt atccatccaa
     2761 aatgaatggg tagataaaaa taataacatt tactttattt tcagtaataa acatttgtgc
     2821 gaatacttag gttatgcaga acaaaaaatt ataaaattaa aaaaagagtt aataagtttt
     2881 aatttactaa ctcaagaacg tgttggcctt aataaaccaa atagattata tttattaaaa
     2941 cctaattatg acattaaagc cagtcatagc aaggaacttc caaattcaca gttccagaac
     3001 aatgaatttg gaagttctag aactgtgaat ttaagtggtc aagaacttcc aaattcacag
     3061 tctaatgata ctgagaataa tgatactgat tatattaaaa ctgagagtaa tgatacgaat
     3121 gatttgaatg ataataaact aacttttcct agtaatcata caaatcattc aaatcacgac
     3181 aattcaaact ttaataatga agctttaaaa ttccaattac tcgaagaatt accacaaagt
     3241 attaaaaact atctaagtaa ctttgaagta gctgaaatta aaattatcaa aactgtatta
     3301 ttaaaagcca aaacatcttt caataatgcg attgatagct actacttgtt agaagatatg
     3361 gaaatagaaa tacttcatgt tcttaaacgt ttcaaagcta tgcttattca aaaaaatgaa
     3421 actgttgaat caatgcaagg atacttaatg aaatctctta agtctgaatt cgctgaaatg
     3481 catacgctta ataaacgacg tgatcattta cccatcactt ctttatttaa tcaataatga
     3541 ttgatttaga gagcatcttt aaatagatgt tctctttttt ttattaacag gtggttgcta
     3601 aagcaaccac ctagatcaaa cgaaaggtaa gggaggagat aacaaaataa catcaataca
     3661 atttcacaac atagcatgct gctattaaag aaaggaggaa gacttatgag tactaagaaa
     3721 aagattaaaa ttactttggc aatgattaat gttcttttaa ctgcagtcga actttatctt
     3781 caatggcaat tgagtaaatc tgaaaatgaa ctgtagttaa gcaagtaggt aggactaaaa
     3841 cgtttctgtt ttagtcctat ttttttctat aaacggttat tattcgtttg aaaatggctc
     3901 aaaaacgtcg cataagatga aaaataaaat gattatttaa cgttgcttta actttaaaga
     3961 atataaaaac actaaccgtt tttatattct ttagcaatct ttcttaatta taaaagtgac
     4021 ttaataaaat cgtttttgtc atttattttg ttaatgatcg gaaagaaaaa gttgaattta
     4081 tcattccata aaatcaaaat tcagattcaa ttgatataca tcataaacaa aaaataagtc
     4141 gttaatgttc aaaacatggg tcgaatgtaa ttttaaattt gattttttct atcccaaact
     4201 aagtaactat ttttacttag ttttctcttt ttttatctcc aattatttta ctaacaatgc
     4261 ttattttgaa aataaaaata agcacttaaa gtaaaaagtg aacaagagaa tagccacttt
     4321 tgtcttaaag cacgttttgt ttttggaaat attacaaaca taataggatt ttcaaatagt
     4381 atttgacatg agcataagga aatccagtac cacctttggt ggtattcagc atagctgaag
     4441 gattgtctta ccgttcgttt tgcttacttg ctctttgaaa ttcactacaa gtgttgatat
     4501 aacaacattt atagttaata ttaaataaaa attaatcgac attataaacc ttaatattgc
     4561 atactattta caattgttac aagaccactt taaattgttt gaatactatt atttttgttg
     4621 atttcatagg cttcagtcga cgtcaatcct ataaatagga ttaatttttt taaccaaatc
     4681 ctattaatcc tactactttt agtattaatt tcaacaacaa aatcactaaa aaagtaatct
     4741 aagcaattca tacatttgtt tcactttttg aaattgatta aatacttttt tctcaatatc
     4801 aaattcaata atgatcatat tatttatttt taagttcaaa aaaagagaaa gaataaaaca
     4861 atttctatac gtttctatgc tcaattttat taaaaagtga atcgaaataa agatcatttt
     4921 atctttttat taactagaga attaaaaaat ataaaatgca taataagatt catcgaaagg
     4981 attatactca aaatttttta ttttaatctt cctgaatttt aaattcaact cttatttacg
     5041 ttttaagaga tatgaaatta tttttttgta taacaatata taacattcta attattttag
     5101 tgagtatcaa gattatttta attaacttta aaatgttaaa taaaaagcaa agtattattt
     5161 attttagact gtctaaagaa ataaaataga acggaaaaca tgccaaatgg cattttctaa
     5221 aagaaggtga tttgacagta tatttttata tttggwttaa aaaagagtat ttagcgagtg
     5281 aaatttttga tttaacagaa agtgaaaaac aagaaattaa tcatcaaatg attgatgaaa
     5341 ttgaagaaga aaaataaaaa taaattttct taaacgttag caaaaagcta gcgttttttt
     5401 gttgaaaaca cgctaaaaat ggctgggtac attttgggta cagacacaat atgatgtggg
     5461 tacaaaatgg gtacaacaca atatcttgtg ggtacattct gggtacaaaa caaaaaaatg
     5521 tatacctttt taaaatctct gtatcccttg aaatgtctgg cttcgccaga cctaccattt
     5581 aaaatggtag caaatttccc ttatgctctt acaaagaatt tggagccaat tcaaaattcg
     5641 gkggtttttt gaaagaaatt gctcctgcag gaactaaaaa agttgatgat tttttgagcc
     5701 attttatgca aaattttagt ttgcatgtaa ctgggcagtg tctaaaaaat cgtcactggt
     5761 tttgctcaga tttttgtttt tggaattaaa atatatttat taggctctca tttgcgtttt
     5821 aagagccaat ataagttttt gggtataaaa ctatataaat aactctaatt ttttaaaata
     5881 ccgtcttaaa attaaaaata aaggtattct gatttgtaat aaattagaat ataaaaaaag
     5941 agccaccatt taaaatttag gcagctctta ctcacttttt ggatgataaa agtggaaaaa
     6001 taatacaaga aaaaacagaa aagatatttt aattataaca cggcatttat gccgagaaaa
     6061 tttattgatc gttgagaaga acccttaact aaacttgcag acgaatgtcg gcatagcgtg
     6121 agctattaag ccgaccattc gacaagtttt gggattgtta agggttcaga ggctcaacgt
     6181 caataaagca atatacatta tttaaggaaa gtgttaaaat ataatcaaat atataaaaat
     6241 gtccccgggc atagaagtgg taataaatat ttcattaggg cacttgttta agtgagaaat
     6301 atttattacc actatttttt tggaggttga atatgaatat ttttgtaaaa aatactaaag
     6361 atttaaaaat agatgatttg ctgaatatat tacgagaaag aattagagtt tttgtaatcg
     6421 aacaaaattg tgtttatgaa gagattgata agaatgataa taatgctatt catgttgtat
     6481 tttatgatca agataaaatt attgcttatt caagaattat tgaacatgaa actttttatt
     6541 catttggacg tgttttagtt gtaaataaat atagaaaaaa aggattaggt aaaaaagttg
     6601 ttagtagaac tcttcgagaa attaaaaaac ttcaaaatga aaattctatt aaaaaaccta
     6661 taaaaattca ggctcaagct tatttagaaa atttttataa gacttttggt tttgtaagta
     6721 aatctgatat ttatcttgag gatgatatac ctcatttgga tatggaaaaa taagtttata
     6781 agactgaatt gttttgtata gaaacgagtt aaaaaacgag tttcttctta tcttgatact
     6841 ataaaaaaag caaagatata acataaaatg acttatcgga agtcagctat tatcacatat
     6901 taagagatga tataatatac aggtcgccta tctctcaggc gtcaatttag acgcagagag
     6961 gaggtgcata tcagatggtc gaagctcttg ttcacatcac gaccacagtc atcagtggtt
     7021 gtattgttgc gttatttacg cattggctac gtacacgtaa caataagaaa aaataggcga
     7081 tattagccag cacctaaaaa atcccctcac tattgcgagt agtgagggga ttggtgcata
     7141 taatgcacga agctcttgtt gtctaaatta taacactggt tagtaaaaga atgcaaaaca
     7201 cttatattaa aaaatgggta tctttgagta gtatcataag ttatagtata gaaatattta
     7261 agtgtttgta ttcagaattt agttttccgt taaatgatgt attgctagaa ggtactgata
     7321 aaaaagcgag taaagaatat ataaacaaga atcaaaaaac tttttattgt ctataatcat
     7381 ctattacttt ctttttacta ataacttcga taattgcaaa cagtatcatt ggttattaaa
     7441 aaaataaaag atgcaaaaat ggctaaatat tggaaagaaa atcaagaggg attattaaat
     7501 gatggggaag cattaactat attaatgatg ttatataatg agcatccata tgtagtacaa
     7561 ttaccgtgtt taaataagca actacaagat gacaacagat acgctcaacc cacgtatact
     7621 atatattcta caaccaaaaa cagaaaagag aataaaagaa gaataaaaaa tagcatataa
     7681 ccttaataag ataattaatt ttgttatatt aaaataatat atagataatt taattattta
     7741 aagtcgaaca attaaaaaag tctgggtaat aacccagact tttttaattt aaataatgag
     7801 tcctattttg tagtatcagc gacagactgt tttaaatcat gccaaccatt tgaaatggca
     7861 ttaccaatac tagtgacttc gccagtgact tcaccaacaa tccctttacc accattgata
     7921 tcttttaatt gtttttcatt tagkgttttc attgaaatag tcctccttta aattattttc
     7981 ctcgtacttt attaattgct ttaccaatac cttgtccgat attgcacatt attccatctt
     8041 ctacaaaatc accagaacaa ccagtttcac gaccaccatt gatatctttt aattgttttt
     8101 catttagtgt ttccattgaa atagtcctcc tttaagtaat ttcatcttac atcttataaa
     8161 ttagcatttg aatataaaga gaatgtaaat agacagttaa gtgagctttt ttttgaattt
     8221 atttttttta ttatcgaaat atatttaata aatagagtat attaatataa aatcagtcta
     8281 ttttcatttg aagttgccat aaatttttat atataccgtt taaatgaatc aactcatcat
     8341 gtgtaccatt ctcagcaatt tgaccctcgt ttaaaacata gatattatca gcatttttta
     8401 tagtacttaa tttatgtgaa ataataatta cggttttacc atgttgagtt agtaagtcaa
     8461 tatgctccat aattttgttt tcagttaaac tatctaaggc acttgtagct tcatcgaaaa
     8521 tatatatatc aggatctttt aaaaatgtac gtgctaaaga taagcgttgg ccttgtccgc
     8581 ccgatagatt agctccttct ttttctaaca tagtgttaaa ctgatctggg aatttctgta
     8641 taaaatctaa ggattctgct aattgacaag ctttaacgat atcttcatca cttttgtatt
     8701 tattgctacc atgtaaaaga ttgtttttaa ttgtatcagc aaaaaggaat gtgttttgtg
     8761 aaacatagcc tatattctta cgtaagtcag aaagatcaat atcatcaata atatagttat
     8821 caatcataat attaccttct gaagcagtgt aatagcgatt gagtaactta cctatagttg
     8881 atttaccact accactttca ccaacaatag caaccttttg ccctttttta atatttaaat
     8941 taatatcctt caataccaca cttcgaaagc catactgaaa gtttacattt tcaatgcgaa
     9001 tgttgttttt aaaagtataa ggttgtttag aagtatataa ttcagtttcg gtctctaaat
     9061 cagttatttc agcaatacga cgtgctgcaa caaatgaaga ttgcagtgtt ggttgaatat
     9121 ttatgagacg ttcaatagga tcaaggtaat aaatagttaa ggcattaaaa gctaataaac
     9181 tacctaacgt catactatcc gtcatgacta agtaactacc aagccacaat ataacaagac
     9241 tgatagttaa ttttaaaaag ttgttaaccg ttaattgtat attagaaaat ctacccagtt
     9301 ttaawaagtt ttcaataata ccattaaatt tagtagtacc aatgtagaaa cgatcgtctt
     9361 cagattgata acttttaata gtattactac catcaaagga ttctattaaa tatgaactca
     9421 attcagcatc tttttcagcc accttttgat tatatttttc aaaaggttta cgtaaagtgt
     9481 aactacaaat aataaaacaa ggtataaaaa taattgttat cagtagtaac aaaggacttt
     9541 gcatataaag taatatagca ccgataataa tcatgaaagt atcaactagc aacgttaccg
     9601 ttgaagatga taaagcttca cgaattttag atgtatccat gaaacgttgt aggatttcgc
     9661 cagatttacg tgtatcaaaa aagttcatcg gaagatgtaa aacatgataa aaataatctt
     9721 tcattaaatt aacatcgatt ttcaaactta aatgtaaaat aagttgaaac cttaaataat
     9781 taatgagtgc attgattatg tataaaagta aaataccgaa gctaataata tgtaaatttt
     9841 taagaacatt ggatggaata atatgatcgg ttaataattt aaaatataga gccccgacaa
     9901 taccaacaat attaataaat agtgagataa aagcaataaa taacaacttt ccataattat
     9961 ttttaaatat atcaataaaa atattaagat acgagggtga ttcattgtca ttagtaaaat
    10021 catctgtttt atcaattaat acagcaatat tagtccaata ttttccaaat tcagtacttg
    10081 ataacttata tttaccttta gcaggatcga caatataaag cgtgttattt tttatttttt
    10141 caataatgat atagtgatca taaccttgtt gattaataat atgtaccata actggtaatt
    10201 gtgcttgttt taaatcatca aaggaatcag cttttacgcc agttaactca ataccaatct
    10261 ttttacctgc ttgtacgaga ccatataaat ttgtacctaa tttatctgtt ttagcatatt
    10321 cgcgcaaacg tgggatagat acacgcttac cataaaactg tgaaatcatg gcaaggcaag
    10381 taggaccaca atccttttca tcgtattgtc ttataaattt tatagccatc ataatgagtc
    10441 ctttcaataa aatttaactc tattttaata agtataaata gttagtatta aacataagtt
    10501 aaataatcct tttgataatg ttaaaagcgc taaaaaataa aaatatttgt ctatatgtct
    10561 acttgtggta caccctaatc atacagatat agttattgaa tttacagcaa ctatgtcact
    10621 ttattcgata tatgagaatg actttaccta ataaatgaga aaagcaacgc actatattaa
    10681 aatgcgttgc tttttctatt atcgaatacc tataactaaa ctatctctag ctgtttctac
    10741 aacttctgta cttattttat ct
//
