LOCUS       CP027489                 655 bp    DNA     linear   BCT 19-MAR-2018
DEFINITION  Staphylococcus aureus strain ST2594 isolate 004 plasmid unnamed4.
ACCESSION   CP027489
VERSION     CP027489.1
DBLINK      BioProject: PRJNA436877
            BioSample: SAMN08634544
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Bastos,L.R., Martins,M.C.F., Albano,R.M., Marques,E.A. and Leao,R.S.
  TITLE     Whole genome sequencing of ST2594 MRSA strain causing skin
            colonization and low-grade infection in surgical site. Implications
            for MRSA pathogenesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 655)
  AUTHORS   Bastos,L.R., Martins,M.C.F., Albano,R.M., Marques,E.A. and Leao,R.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2018) Departamento de Microbiologia, Imunologia e
            Parasitologia, Universidade do Estado do Rio de Janeiro, Av. 28 de
            Setembro, 87, Vila Isabel, Rio de Janeiro, RJ 20551-030, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.5; MAUVE v. 2.4.0
            Assembly Name                     :: Saureus141ST2594
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: BA000018.3
            Genome Coverage                   :: 40.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/06/2018 13:58:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.4
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,922
            CDS (total)                       :: 2,883
            Genes (coding)                    :: 2,764
            CDS (coding)                      :: 2,764
            Genes (RNA)                       :: 39
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 3 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 65 of 119
            Pseudo Genes (incomplete)         :: 37 of 119
            Pseudo Genes (internal stop)      :: 29 of 119
            Pseudo Genes (multiple problems)  :: 12 of 119
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Lab2_UERJ_19239.
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ST2594"
                     /isolate="004"
                     /isolation_source="Femural surgical wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed4"
                     /geo_loc_name="Brazil: Rio de Janeiro"
                     /lat_lon="22.8951 S 43.2431 W"
                     /collection_date="2015-03-11"
                     /collected_by="Leonardo Bastos"
     gene            <1..478
                     /locus_tag="C6V85_14260"
     CDS             <1..478
                     /locus_tag="C6V85_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit M"
                     /protein_id="AVO70312.1"
                     /translation="RGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKC
                     RQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKATIDKYSYSATLQEIADND
                     YNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE"
                     /name="type I restriction-modification system subunit M"
     gene            471..>655
                     /locus_tag="C6V85_14265"
     CDS             471..>655
                     /locus_tag="C6V85_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="AVO70313.1"
                     /translation="MSNTQTKNVPELRFPGFEGEWEEKKLGNLTTKIGSGKTPKGGSEN
                     YTNKGIPFLRSQNIRNG"
                     /name="restriction endonuclease subunit S"
ORIGIN
        1 ccgtggtgcc gcagaaggtg tgattcgtcg ctatttaata gaagaaaaga actacttaga
       61 agccgtgatt gggttaccag ccaatatttt ctatgggaca agtattccaa catgtatttt
      121 agtatttaaa aaatgtcgcc aacaagacga caacgtacta tttatcgatg catccaatga
      181 ttttgaaaaa ggaaaaaatc aaaaccattt aagcgatgcc caagtcgaac gtattataga
      241 cacatataag cgtaaagcaa cgattgataa atatagctac agcgcgacat tacaagagat
      301 cgccgataac gattacaact taaacatacc gcgatatgtt gatacattcg aagaagaagc
      361 accaattgat ttagatcaag tccaacaaga tttgaaaaat atcgacaaag aaatcgcaga
      421 aattgaacaa gaaatcaatg catacctgaa agaacttggg gtgttgaaag atgagtaata
      481 cacaaacgaa aaatgtgcca gagttgagat tcccaggatt tgagggcgaa tgggaagaga
      541 agaagttagg gaatcttact accaaaatag gtagtggaaa gactcccaaa ggtggaagtg
      601 aaaactatac aaacaaaggc ataccatttt taaggagtca aaatattaga aatgg
//
