LOCUS       CP027488                2687 bp    DNA     linear   BCT 19-MAR-2018
DEFINITION  Staphylococcus aureus strain ST2594 isolate 004 plasmid unnamed3.
ACCESSION   CP027488
VERSION     CP027488.1
DBLINK      BioProject: PRJNA436877
            BioSample: SAMN08634544
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Bastos,L.R., Martins,M.C.F., Albano,R.M., Marques,E.A. and Leao,R.S.
  TITLE     Whole genome sequencing of ST2594 MRSA strain causing skin
            colonization and low-grade infection in surgical site. Implications
            for MRSA pathogenesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Bastos,L.R., Martins,M.C.F., Albano,R.M., Marques,E.A. and Leao,R.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2018) Departamento de Microbiologia, Imunologia e
            Parasitologia, Universidade do Estado do Rio de Janeiro, Av. 28 de
            Setembro, 87, Vila Isabel, Rio de Janeiro, RJ 20551-030, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.5; MAUVE v. 2.4.0
            Assembly Name                     :: Saureus141ST2594
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: BA000018.3
            Genome Coverage                   :: 40.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/06/2018 13:58:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.4
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,922
            CDS (total)                       :: 2,883
            Genes (coding)                    :: 2,764
            CDS (coding)                      :: 2,764
            Genes (RNA)                       :: 39
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 3 (5S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 65 of 119
            Pseudo Genes (incomplete)         :: 37 of 119
            Pseudo Genes (internal stop)      :: 29 of 119
            Pseudo Genes (multiple problems)  :: 12 of 119
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Lab2_UERJ_19239.
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ST2594"
                     /isolate="004"
                     /isolation_source="Femural surgical wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed3"
                     /geo_loc_name="Brazil: Rio de Janeiro"
                     /lat_lon="22.8951 S 43.2431 W"
                     /collection_date="2015-03-11"
                     /collected_by="Leonardo Bastos"
     gene            <1..91
                     /locus_tag="C6V85_14235"
     CDS             <1..91
                     /locus_tag="C6V85_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="AVO70308.1"
                     /translation="IYALYKKNRRSLQIYGFSPCHEISIMLAS"
                     /name="IS6 family transposase"
     gene            232..867
                     /locus_tag="C6V85_14240"
                     /pseudo=""
     CDS             232..867
                     /locus_tag="C6V85_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing start; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /name="aminoglycoside 6-adenylyltransferase"
     gene            864..1406
                     /gene="sat4"
                     /locus_tag="C6V85_14245"
     ARG             865..1403
                     /name="sat4"
                     /gene="sat4"
                     /gene_name="streptothricin N-acetyltransferase Sat4"
                     /tool="amrfinderplus"
                     /drug_class="STREPTOTHRICIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1499..2293
                     /gene="aph(3')-IIIa"
                     /locus_tag="C6V85_14250"
     ARG             1500..2290
                     /name="aph(3')-IIIa"
                     /gene="aph(3')-IIIa"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(2598..>2687)
                     /locus_tag="C6V85_14255"
     CDS             complement(2598..>2687)
                     /locus_tag="C6V85_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="AVO70311.1"
                     /translation="IYALYKKNRRSLQIYGFSPCHEISIMLAS"
                     /name="IS6 family transposase"
     ARG             865..1406
                     /name="SAT-4"
                     /gene="SAT-4"
                     /gene_name="streptothricin acetyltransferase (SAT)"
                     /tool="rgi"
                     /drug_class="nucleoside antibiotic"
                     /identity="99.44"
                     /coverage="100.0"
     ARG             269..867
                     /name="aad(6)"
                     /gene="aad(6)"
                     /gene_name="ANT(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.5"
                     /coverage="72.1"
ORIGIN
        1 tatttacgct ctatataaaa agaaccgtag gtctcttcag atctacggat tttccccatg
       61 ccacgaaatt agcatcatgc tagcaagtta aacgaacact gacatgatat attagtgatt
      121 agctatattt tttactttgc aacagaacca tcatttttat taaaatactc ttttgtaaaa
      181 tctttctgct cttcaatgta tttaatttct ttacctctaa atctattata gctatttgat
      241 gattacaata aaattgatct taccttattg cccttggaag agttagataa ttacctaaag
      301 ggcgataaat taataaaggt tctaattgat aaagattgta gaattaaaag ggacatagtt
      361 ccgactgata tagattatca tgtaagaaag ccaagcgcaa gggagtatga tgattgctgc
      421 aatgaatttt ggaatgtaac accttatgtt attaaaggat tgtgccgtaa ggaaatttta
      481 tttgctattg atcattttaa tcagattgtt cgccatgagc tgctgagaat gatatcatgg
      541 aaggtcggca tcgaaacagg ctttaaatta agtgtaggca agaactataa gtttattgaa
      601 aggtatatat ccgaggattt gtgggagaaa cttttgtcca cctaccggat ggattcctat
      661 gaaaacatat gggaagcatt atttctatgc catcaattgt tcagggcggt atccggtgag
      721 gtggcggaaa ggcttcatta tgcctatccg gagtatgata ggaatataac aaaatatacc
      781 agggacatgt ataaaaaata cactggtaaa accggctgcc tggatagcac atatgccgct
      841 gatatagaag agaggcggga acagtgatta cagaaatgaa agcagggcac ctgaaagata
      901 tcgataaacc cagcgaacca tttgaggtga taggtaagat tataccgagg tatgaaaacg
      961 agaattggac ctttacagaa ttactctatg aagcgccata tttaaaaagc taccaagacg
     1021 aagaggatga agaggatgag gaggcagatt gccttgaata tattgacaat actgataaga
     1081 taatatatct ttactaccaa gacgataaat gcgtcggaaa agttaaactg cgaaaaaatt
     1141 ggaaccggta cgcttatata gaagatatcg ccgtatgtaa ggatttcagg gggcaaggca
     1201 taggcagcgc gcttatcaat atatctatag aatgggcaaa gcataaaaac ttgcatggac
     1261 taatgcttga aacccaggac aataacctta tagcttgtaa attctatcat aattgtggtt
     1321 tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaacaac tttgaaaaag
     1381 ctgttttctg gtatttaagg ttttagaatg caaggaacag tgaattggag ttcgtcttgt
     1441 tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga aataataaat
     1501 ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa
     1561 agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa atgaaaacct
     1621 atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg aacgggaaaa
     1681 ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc actttgaacg
     1741 gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt gctcggaaga
     1801 gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt gcatcaggct
     1861 ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca gccgcttagc
     1921 cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa actgggaaga
     1981 agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga
     2041 agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg tgaaagatgg
     2101 caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt ggtatgacat
     2161 tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg tcgagctatt
     2221 ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata ttttactgga
     2281 tgaattgttt tagtacctag atttagatgt ctaaaaagct ttaactacaa gctttttaga
     2341 catctaatct tttctgaagt acatccgcaa ctgtccatac tctgatgttt tatatctttt
     2401 ctaaaagttc gctagataga gttctatatt agagatgtaa agagttttgg tgaagaggtt
     2461 tgtgagtatg gcttttataa ccagggtgca atgagaagca ctaacagcag ctagcttaag
     2521 aacgcatagg atatacttgg ttctgttgca aagtaaaaaa atatagctaa ccactaattt
     2581 atcatgtcag tgttcgctta acttgctagc atgatgctaa tttcgtggca tggcgaaaat
     2641 ccgtagatct gaagagacct gcggttcttt ttatatagag cgtaaat
//
