LOCUS       CP026191               12794 bp    DNA     circular BCT 29-JAN-2018
DEFINITION  Enterobacteriaceae bacterium ENNIH2 plasmid pENT-6b61, complete
            sequence.
ACCESSION   CP026191
VERSION     CP026191.1
DBLINK      BioProject: PRJNA430813
            BioSample: SAMN06040391
KEYWORDS    .
SOURCE      Enterobacteriaceae bacterium ENNIH2
  ORGANISM  Enterobacteriaceae bacterium ENNIH2
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Weingarten,R.A., Johnson,R.C., Conlan,S., Ramsburg,A.M.,
            Dekker,J.P., Lau,A.F., Khil,P., Odom,R.T., Deming,C., Park,M.,
            Thomas,P.J., Henderson,D.K., Palmore,T.N., Segre,J.A. and Frank,K.M.
  CONSRTM   NISC Comparative Sequencing Program
  TITLE     Genomic Analysis of Hospital Plumbing Reveals Diverse Reservoir of
            Bacterial Plasmids Conferring Carbapenem Resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12794)
  AUTHORS   Segre,J.A. and Mullikin,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2018) NISC, NIH, 5625 Fishers Lane, Rockville, MD
            20852, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 240x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/22/2018 15:34:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,243
            CDS (total)                       :: 6,124
            Genes (coding)                    :: 5,934
            CDS (coding)                      :: 5,934
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 70 of 190
            Pseudo Genes (incomplete)         :: 104 of 190
            Pseudo Genes (internal stop)      :: 53 of 190
            Pseudo Genes (multiple problems)  :: 30 of 190
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            Bacteria available from Karen Frank, Department of Laboratory
            Medicine, National Institutes of Health Clinical Center.
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12794
                     /organism="Enterobacteriaceae bacterium ENNIH2"
                     /mol_type="genomic DNA"
                     /strain="ENNIH2"
                     /db_xref="taxon:1920109"
                     /plasmid="pENT-6b61"
                     /geo_loc_name="USA"
                     /lat_lon="38.984652 N 77.094709 W"
                     /collection_date="2016"
     gene            join(10380..12794,1..483)
                     /locus_tag="C2U52_01745"
     CDS             join(10380..12794,1..483)
                     /locus_tag="C2U52_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="AUV05104.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            complement(1359..2255)
                     /locus_tag="C2U52_01750"
     CDS             complement(1359..2255)
                     /locus_tag="C2U52_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AUV05105.1"
                     /translation="MKSLDDLIARLPALQEKARQRAAQEQASAPAKVVQLPLWPEPVRA
                     VPNGFLRSALFGAIAKGKRRYIDGEQLAALDGIEIRYTGQRLDQGDLDVWESVLHAVRL
                     QELGSQCRLTSYGLLKLMGKTDTGKNRATLHKRITRLRAGTVELKQGRYVYIGGLIGEA
                     FKDEETQEWVIELNPKLRALYGGDQFTQVDWGVRHALDGRQLAQWLHGFYASHAKPFPM
                     RMETLLKLAGSEDANPRSAQQTLRKALEAVAEASEANGEGFSYEIRGDLVHVEKKASGT
                     QRLHLAKKAAQRRKPRT"
                     /name="hypothetical protein"
     gene            complement(2242..2439)
                     /locus_tag="C2U52_01755"
     CDS             complement(2242..2439)
                     /locus_tag="C2U52_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AUV05106.1"
                     /translation="MKDAKDKKTGDLLASSNSRRQAAYAERQRALGRKKFSYWMRPDEA
                     EQVAAFLERLRPQEAEHEKP"
                     /name="hypothetical protein"
     gene            complement(2436..2642)
                     /locus_tag="C2U52_01760"
     CDS             complement(2436..2642)
                     /locus_tag="C2U52_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AUV05107.1"
                     /translation="MTDEALNALFGKADYSHIAHDATVTVSITAAEMAALLGAYDRGLD
                     ALDQDERDGLNAVIGKLKDELWP"
                     /name="hypothetical protein"
     gene            complement(3469..4335)
                     /locus_tag="C2U52_01765"
                     /pseudo=""
     CDS             complement(3469..4335)
                     /locus_tag="C2U52_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044869.1"
                     /note="incomplete; partial on complete genome; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            4521..4784
                     /locus_tag="C2U52_01770"
                     /pseudo=""
     CDS             4521..4784
                     /locus_tag="C2U52_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451932.1"
                     /note="incomplete; partial on complete genome; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /name="mobilization protein"
     gene            4862..5044
                     /locus_tag="C2U52_01775"
     CDS             4862..5044
                     /locus_tag="C2U52_01775"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AUV05108.1"
                     /translation="MTQQQPPNCYRSRPADDGGLYLLMPRDEHLCPPLPAPVPVVLELP
                     AAECGALPHAPHSPP"
                     /name="hypothetical protein"
     gene            5499..6503
                     /locus_tag="C2U52_01780"
     CDS             5499..6503
                     /locus_tag="C2U52_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_569408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS4321 family transposase"
                     /protein_id="AUV05109.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDRRGKAVYRKKFTRPKLIEFLATC
                     PATTIAMEACGGSHFMARKLEELGHSPKLISPQFVRPFVKSNKNDFVDAEAICEAASRP
                     SMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISRLST
                     ILEDNSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCVGTLT
                     ASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLLVQCAR
                     VFIQKLEHQSGKLADWVRDLLCRKSNFVVTCALANKLARIAWALTARQQTYVA"
                     /name="IS110-like element IS4321 family transposase"
     gene            complement(6685..6780)
                     /locus_tag="C2U52_01785"
                     /pseudo=""
     CDS             complement(6685..6780)
                     /locus_tag="C2U52_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="incomplete; partial on complete genome; missing
                     start; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /name="integrase"
     gene            6853..8049
                     /locus_tag="C2U52_01790"
     CDS             6853..8049
                     /locus_tag="C2U52_01790"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011061617.1"
                     /note="enzyme from Treponema denticola exhibits
                     NADH-dependent trans-2-enoyl-CoA reductase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabV"
                     /protein_id="AUV05110.1"
                     /translation="MVIKPRVRGFICVTTHPVGCEANVKQQIDYVTQQGAIDAGPKNVL
                     VLGASTGYGLAARITAAFGCGANTLGVFFERGSVDGKLGTAGWYNTAAFHKFAEAKGLY
                     AKSINGDAFSDAVKAETIETIKRDLGKIDLVVYSLAAPRRTHPKTGEVLTSTLKPLGKS
                     VTLRGVDTDKEIVKETTLQPATQEEIDGTVAVMGGEDWQMWIDALHEAGVLAEGAKTTA
                     FTYLGEKLTHDIYWNGSIGEAKKDLDHKVIGMREQLADLGGDARVSVLKAVVTQASSAI
                     PIMPLYLSLLFKVMKEQGSHEGCIEQVYGLYKDSLYNSEPVIDEEGRLRADYKELQPQV
                     QDRVKQLWDQVTSENLYEMTDFVGYKQEFLRLFGFEIPGVDYEADVDPDVKISNLIQL"
                     /name="enoyl-[acyl-carrier-protein] reductase FabV"
     gene            complement(8109..8492)
                     /locus_tag="C2U52_01795"
     CDS             complement(8109..8492)
                     /locus_tag="C2U52_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009048657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="AUV05111.1"
                     /translation="MEAVYFLEVHQARNSSRRGNKIPFRRRIDSSKPTCRQNRCCKNGS
                     DHRFRFLKRPLTDVSIPTGARWIPKGMSVEYLAKAKGNVRAVADGSAVDWASAGDKLVE
                     VTALNEEDQAVFTARITMNVKLA"
                     /name="DUF4442 domain-containing protein"
     gene            complement(8604..9608)
                     /locus_tag="C2U52_01800"
     CDS             complement(8604..9608)
                     /locus_tag="C2U52_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_569408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS4321 family transposase"
                     /protein_id="AUV05112.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDRRGKAVYRKKFTRPKLIEFLATC
                     PATTIAMEACGGSHFMARKLEELGHSPKLISPQFVRPFVKSNKNDFVDAEAICEAASRP
                     SMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISRLST
                     ILEDNSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCVGTLT
                     ASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLLVQCAR
                     VFIQKLEHQSGKLADWVRDLLCRKSNFVVTCALANKLARIAWALTARQQTYVA"
                     /name="IS110-like element IS4321 family transposase"
     gene            complement(9680..10285)
                     /locus_tag="C2U52_01805"
     CDS             complement(9680..10285)
                     /locus_tag="C2U52_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA resolvase"
                     /protein_id="AUV05113.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="DNA resolvase"
     Typing          complement(1360..2255)
                     /name="rep_cluster_2147"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gatattttcc gttgtgccgc gacgatgacc gctggcaaaa tcaggcccag ccaactcctg
       61 cgcaagctcg cttcttaccc acgacaaaac aaccttgcag ttgcgcttcg tgaagttggt
      121 cgtattgaac ggaccctttt cattattgag tggatcctgg atacggacat gcagcggcgt
      181 gctcagatcg gtcttaacaa gggagaggcc caccatgcgc tcaaaaatgc gctccgtatc
      241 gggaggcagg gggaaattcg cgatcgcacg acagaggggc agcactaccg aatcgctggg
      301 ctcaatttat tgactgcggt gatcatttac tggaataccg tccatcttgg tcatgccgtc
      361 acggagcggc ggaacgaagg gttggatgtt ccccctgaat ttcttcccca catatcccca
      421 ttgggctggg cgcacattct actgactggc gaatatcttt ggcccaagga accgaaagct
      481 tagggtgtca tttcgccctc agccggaacc gacccctagt accgggaagg agggagtgac
      541 catctcatta aaaatcgtac gctaagggac acaaggtgtc aggttgtggc catagaccgc
      601 gcatcaatgg tgtccacaaa ttcggaaaac ggctctactc tgacggtttt acctctctgg
      661 tctgacgtcc agttggggca tgccccagac tgacaccgcc aacgtcatac cagagctggc
      721 aggtgggtgg aacgacggct tagcgtgctt tattttccgt ttcctgaagc gacccccatc
      781 cgctaaacac cccgccaaac gccctaaaac ggcctacagc gcgttttctc accccaaccc
      841 tgccggatgt accccgaagc ctgcaaagcg gctcctaggg cttcctgtgg ccgttcctgc
      901 gcattcctgc ccctcccctt tcattgccaa gccatagcgg ggtgcctgcg tggccgttca
      961 ggccgcgcaa gggggcgcag ccacaggggg gccggggggc gtgccgcccc acgtcgagga
     1021 gcgccctagg cgcgggctgt aagccggtat ttgggggcca gttcgccccc tctgggggcc
     1081 acgatttgcg ggaaggggag ccgccgcgaa gcggtggggg aaccccgcag gggtgccccg
     1141 tgcagggcac gcgatgatta ataaatgatt aaaagaggat taggcgcgtt ttcgccaaag
     1201 gataaaaccc agcactggcg ggggtttacg gcctgtttgc cctgtggata gtgcaggcgc
     1261 tggaacggta tccggcaggc gctggaacgg tatccggcag gcgctggaac ggtatccggc
     1321 aggcgctgga acggtatccg gcaggcgctg gaacggtatc aggtgcgggg cttccggcgc
     1381 tgcgctgcct tcttggccaa gtgcagccgc tgcgttccgc tggccttctt ttcgacgtgc
     1441 accaggtcgc cgcgaatctc gtagctaaac ccctcgccgt tggcctcgct ggcctctgcc
     1501 acagcctcca gcgccttgcg tagcgtctgc tgggcgctgc gcgggttggc gtcctcgctg
     1561 cccgccagct tgagcagcgt ttccatgcgc atcgggaacg gcttggcgtg actggcgtag
     1621 aagccgtgca gccactgcgc gagctggcgg ccatccagcg catgacgcac gccccaatcc
     1681 acctgtgtga actggtcgcc gccgtacaag gctcgcaatt tcgggttcag ctcaatcacc
     1741 cattcctgcg tttcctcgtc cttgaatgcc tcgccaatca ggccgcctat gtacacgtac
     1801 cggccttgtt tcagctcaac ggttccagct cgaagccgcg tgatgcgctt gtgcaacgtt
     1861 gcccggttct tgccggtgtc ggtcttgccc atgagtttca acaggccata ggacgtgagg
     1921 cggcattgac tgcccaactc ctgcaagcgc acggcgtgca gcacgctttc ccacacgtcc
     1981 aaatcccctt ggtcgaggcg ttggccggtg tatcggatct cgatcccgtc gagtgcggcc
     2041 agttgctcgc cgtcgatgta ccgacgtttg cccttggcaa tcgcaccgaa caacgccgac
     2101 cgtaggaagc cgttcggcac cgctcggaca ggctccggcc atagcggaag ctgcaccacc
     2161 ttggccgggg cgcttgcctg ctcctgcgcg gctcgctggc gtgctttttc ctgcaatgcc
     2221 ggcaggcggg ctatcaagtc gtcaaggctt ttcatgctct gcttcctgcg gccgcagccg
     2281 ctccaaaaat gcggccacct gctcggcttc gtccgggcgc atccagtatg aaaacttctt
     2341 ccggcctagt gcccgctggc gctcggcata ggccgcctgt cggcggctgt tcgatgacgc
     2401 caagaggtcg ccggtctttt tgtccttcgc gtccttcatg gccacagctc atctttcagc
     2461 ttgccgataa cggcgttcag gccgtcgcgc tcgtcctggt cgagtgcgtc taggccacgg
     2521 tcataagcgc ccagcagcgc ggccatttcg gcggcggtga tgctcacggt aacggtggca
     2581 tcgtgggcga tgtgcgaata atcggctttg ccgaaaaggg cgttcagggc ttcgtcggtc
     2641 atggcgtttt ctccgttaga gataacatca taacacgggt tagagataac gcaagaatta
     2701 tttttaccgc tccatgcccc ggtcgttggc gcggtactgc tgccgctgct cggccagcag
     2761 ctcgcgcacc tgctcgcggc gctggccgtc gctcaagatg cgttcaagga tggccgggcg
     2821 tgccgcattg ggcgcggcgt tgtagccgtc aatcagcttc ttcaagccct cgggcgcagc
     2881 cttccattgc tcgccccggt cgctccagcc tccggccttc atctcgcgct tgacggccat
     2941 tgctgcaaag tccttgggca cggcttcgcg ctcgcgctcg gccttcttct cggcctgctg
     3001 gcgcttctgc tgctcgaact cctggcgcac cttctcccgg cgcagttcct ccagctcggc
     3061 caccttggcc agcgtcggcg cttgctcggc gctgatgcgc tgctgtgcct ggctgcgcag
     3121 gtgctgcacc tgcaagcctg cctgctcgat ctgcggcccc agcttctgcc actcggcccg
     3181 gtttgcctcg cgctgttcct ccagctcgcg cagcttggcc gcgtggccta tgcccttgtc
     3241 gtgcagccag gcttgcacct tgtgcgcttc gcgccattcc tgcgcctggt cgcgggcgcg
     3301 ggctgaggcg ctgcccgctt cggtgtgccg gttctggagc gcctgccgct cttgctctgc
     3361 ctgcaaaacg ccccgatcac gctctaccag gtcaagcgcc ttcggtggcc gaatgcgccc
     3421 gatttcgtgg gccagctcgg tgctgttcat gcgctcgatg cgctggtgtt cggcctgctc
     3481 gcgctcgcgg gcttggcgct cctgctgggt tgccctctcc cgcttggccg cttcaaggtc
     3541 gccactcagg tcgatgattg accggtcggc ctccttccct tggcgttcca actcgcccgc
     3601 atcgcgggca cgctggaggc gctctagcgc gtcctcgttg tgttgctggc gtttgtggct
     3661 gggctggccg gtgcgtcgct cgatggcggt ggccgtcggc ccaaggtggc gggtcgcctc
     3721 ccgctcgatg ccctgcgcct ctaggctgcg gtggtcaacc tgcaccgagt gccccgcttc
     3781 gcgtaggcgc tcgttctgga gcgtggcgaa ccgctcgcgc cattcggtga caagcacctt
     3841 ccccgttttc tggtcgtcca gctcgcgggt tttctcgccg aaaccgtccg gctcaaggcg
     3901 gcgggtcgtt agcaggatgt gggcgtggtg gttgcggttg tcgccttcct tgccgggggc
     3961 atggatggcg acatcagcgg cgcagccgtg gcgcttgacc agctcgcggg cgaagtcgtg
     4021 cgccagcttt tcccgctgct cgggggaaag ctcggcgggt agggctatct caaactcacg
     4081 ggctaccgtg gagtttttgc gcttttcagc ctgctcggcg gcgttccaga gttgggcgcg
     4141 gtcgcgtgcc cactcggggg cgttgtccgg caggatgagg gcggcggatt cgactccacc
     4201 ctttcgggtg tagtcgtgaa tttcgccggt tcgctcgtcg gtgatttcca ccccggcacg
     4261 ataagcagcg gcggcggtcg cagaccggcc agcactgcgg cttatcgttt tgacggatag
     4321 gtggtagatc gccacgggcg cggctccggc tgttggtttt tccccgaagg ggcgcacgca
     4381 aacgaagttt gcataagtgc gcccttggct gttcgcctgc gggtggtcgc tgccgggtat
     4441 tctacccgtc cgctccggct agcgcacccc cagcacggcg ggctaaaatt gcggccatcc
     4501 accgacaaag agggcacgac atggcagcaa tcgacgacaa aatcacaaag ctggaaaccc
     4561 agctcaagca ggccaaagca caacgccaga aggtggaagc cagaaaacgc gctgccacct
     4621 ccaagatgac acgccagcag gacacccgga aaaaaatcct cgttggtgcc gccatcctcg
     4681 ccaaagtgga gcgcggcgag tggccgaaag aaaagctgct cgccatgctg gaaaccacgc
     4741 taacccgtgc cgatgatcgg gcgctgtttg acctgcaagc cccggccact gaacccgctt
     4801 ttctggacga cgtagacccc gacacggtgg acattccgaa gctcgtcacc ctgccccgct
     4861 catgacccag cagcagccgc caaactgcta ccgctcccgg cctgccgacg atggcgggct
     4921 gtacctgctc atgccccgcg atgagcacct atgcccgcca ctgcctgcgc cggtgccggt
     4981 cgttcttgaa ttgccagccg ccgaatgtgg ggcgctgccc cacgccccgc actcgccgcc
     5041 gtgaggctcg gggggcagtc ctggcgggct ttgcccttgt cctgccccca ctcgtaaggc
     5101 tctggccgct cctgtcgcgt caagggttcg gcttcgccca gccctacgcc cggttcctcc
     5161 cttcggtcgg actgcggcct atggtctgcc cttgacccgc catccggggt cgcttcagga
     5221 aacggaaaat aaagcacgct aagccgtcgt tccacccacc tgccagctct ggtatgacgt
     5281 tggcggtgtc agtctggggc atgccccaac tggacgtcag accagagagg taaaaccgtc
     5341 agagtagagc cgttttccga atttgtggac accattgatg cgcggtctat ggccacaacc
     5401 tgacaccttg cgtcccttag cgtacgattt ttaatgagat ggtcactccc tccttcccgg
     5461 tactatgctg aggacaggct ttcattcgga gaactatcat ggaaaacatt gcgctcattg
     5521 gtatcgatct gggtaaaaac tctttccata ttcattgcca ggatcgtcgc gggaaggctg
     5581 tttaccgtaa aaaatttacc cggccaaagt tgatcgaatt tttggcgaca tgccccgcta
     5641 caaccatcgc aatggaagcc tgtggcggtt ctcactttat ggcacgcaag ttggaagagt
     5701 tggggcattc cccaaagctg atatcaccac aatttgtccg cccgttcgtt aaaagcaata
     5761 aaaacgactt tgtcgacgcc gaagctattt gtgaagctgc atcgcgtccg tctatgcgtt
     5821 ttgtgcagcc cagaacggaa tctcagcagg caatgcgggc tctgcatcgt gtccgtgaat
     5881 ccctggttca ggataaggta aaaacaacca atcaaatgca tgcttttctg ctggaatttg
     5941 gcattagcgt tccccgagga gctgccgtta ttagccgact gagtaccatt cttgaggata
     6001 atagtttgcc tctttacctc agccagttat tgctgaaatt acaacagcat tatcactatc
     6061 ttgttgagca gattaaagat ttggaatccc agttgaaacg aaagttggac gaagatgagg
     6121 ttggacagcg cttgctgagc attccctgcg tcggaacact gacagcgagt actatttcaa
     6181 ctgagattgg cgacgggaag cagtacgcca gcagccgtga ctttgcggcg gcaacagggc
     6241 ttgtacctcg gcagtacagc acgggaggta ggacgacatt gctgggaatt agtaagcgag
     6301 gtaataaaaa gatccgaact ttgttggttc aatgtgccag ggtattcata caaaaactgg
     6361 aacaccagtc tggcaaattg gccgattggg tcagggattt actgtgccgg aaaagcaact
     6421 ttgtcgtcac ttgtgctctg gcaaacaagc tggccagaat agcctgggcc ctaacggcac
     6481 gacagcaaac ttatgtagca taacggcaga aatacaccgg tttaaagaat tactgatctg
     6541 gttttgcgaa tactgatatt gatgatacta acggcccacc ggcctgttga ggaacctgta
     6601 aaacggaaag gctcattgaa gccgtatatt ttctggaggt tcatcaggcg cggaactcat
     6661 caaggcgcgg gaataaaatc ccattcagac gccggataga ttcaagcaag ccaacttgtc
     6721 gtcaaaatcg gtgttgcaaa aacgggagtg accatagatt ccgtttcctg aagcgacccc
     6781 gtctacgact tagcaagtga cactgccgcc gcctttattg gcgcctggca cattgaaatt
     6841 gggagattct gtatggtcat caaaccccgc gttcgcggct ttatctgtgt gacgacccat
     6901 ccggttggtt gcgaagccaa cgtcaagcaa cagatcgatt acgtcaccca gcagggcgcc
     6961 atcgacgcag ggccgaagaa cgtactggta ctcggcgcgt ccaccggcta cggcctggca
     7021 gcgcggatca ccgcagcgtt cggttgcggc gccaacaccc tcggggtgtt cttcgagcgc
     7081 ggcagcgtcg acggcaagct gggcaccgcc ggctggtaca acaccgcggc gttccacaag
     7141 ttcgccgaag ccaagggcct gtacgccaag agcatcaatg gcgacgcttt ctccgatgcg
     7201 gtcaaggccg aaaccatcga gaccatcaag cgtgacctgg gcaagatcga cctggtggtc
     7261 tacagcctgg ccgcgccgcg tcgcacccac cccaagaccg gtgaagtgct gacctcgacc
     7321 ctcaagcccc tgggcaagtc ggtgaccttg cgcggtgtcg acaccgacaa ggaaatcgtc
     7381 aaggaaacca ccctgcaacc ggcgactcag gaagagatcg acggcaccgt ggcggtgatg
     7441 ggcggcgaag actggcagat gtggatcgac gcactgcacg aagccggagt cctggccgaa
     7501 ggcgcgaaga ctaccgcttt cacttatctg ggcgagaagc tgacccacga tatctactgg
     7561 aacggttcca tcggcgaagc caagaaggac ctggaccaca aggtcatcgg catgcgcgag
     7621 caactggccg atctgggcgg cgatgctcgc gtatcggtac tcaaggccgt ggtaacccag
     7681 gccagctcgg caatcccgat catgccgctg tacctgtcgc tgctgttcaa ggtgatgaag
     7741 gagcagggca gccacgaagg ttgcatcgag caggtctatg gcctgtacaa ggacagcctg
     7801 tacaacagcg agccggtgat cgacgaggaa ggtcgcctgc gtgccgacta caaggagctg
     7861 caaccccagg tccaggaccg ggtcaagcag ctgtgggacc aggtcaccag cgagaacctc
     7921 tacgagatga ccgacttcgt cggttacaag caagagttcc tgcgcctgtt cggtttcgag
     7981 attccgggtg tcgactacga agccgacgtc gatccggacg tgaagatcag caacctgatc
     8041 cagctgtaac cctgtcgtag cgtttcactc caacgcccct caagcccgag gggcgttgtt
     8101 gtttctgctc aggcgagttt cacgttcatg gtgatccgcg ccgtgaacac cgcctggtct
     8161 tcctcgttga gggcggtcac ttccaccagc ttgtcgccgg cactggccca gtccacggcg
     8221 ctgccgtcgg ccaccgcgcg cacattgccc ttggccttgg ccaggtattc cacgctcatg
     8281 cccttgggaa tccaccgagc accggtgggg atgctgacgt cggtcagggg tcgcttcaga
     8341 aaacggaatc tatggtcact cccgtttttg caacaccgat tttgacgaca agttggcttg
     8401 cttgaatcta tccggcgtct gaatgggatt ttattcccgc gccttgatga gttccgcgcc
     8461 tgatgaacct ccagaaaata tacggcttca atgagccttt ccgttttaca ggttcctcaa
     8521 caggccggtg ggccgttagt atcatcaata tcagtattcg caaaaccaga tcagtaattc
     8581 tttaaaccgg tgtatttctg ccgttatgct acataagttt gctgtcgtgc cgttagggcc
     8641 caggctattc tggccagctt gtttgccaga gcacaagtga cgacaaagtt gcttttccgg
     8701 cacagtaaat ccctgaccca atcggccaat ttgccagact ggtgttccag tttttgtatg
     8761 aataccctgg cacattgaac caacaaagtt cggatctttt tattacctcg cttactaatt
     8821 cccagcaatg tcgtcctacc tcccgtgctg tactgccgag gtacaagccc tgttgccgcc
     8881 gcaaagtcac ggctgctggc gtactgcttc ccgtcgccaa tctcagttga aatagtactc
     8941 gctgtcagtg ttccgacgca gggaatgctc agcaagcgct gtccaacctc atcttcgtcc
     9001 aactttcgtt tcaactggga ttccaaatct ttaatctgct caacaagata gtgataatgc
     9061 tgttgtaatt tcagcaataa ctggctgagg taaagaggca aactattatc ctcaagaatg
     9121 gtactcagtc ggctaataac ggcagctcct cggggaacgc taatgccaaa ttccagcaga
     9181 aaagcatgca tttgattggt tgtttttacc ttatcctgaa ccagggattc acggacacga
     9241 tgcagagccc gcattgcctg ctgagattcc gttctgggct gcacaaaacg catagacgga
     9301 cgcgatgcag cttcacaaat agcttcggcg tcgacaaagt cgtttttatt gcttttaacg
     9361 aacgggcgga caaattgtgg tgatatcagc tttggggaat gccccaactc ttccaacttg
     9421 cgtgccataa agtgagaacc gccacaggct tccattgcga tggttgtagc ggggcatgtc
     9481 gccaaaaatt cgatcaactt tggccgggta aattttttac ggtaaacagc cttcccgcga
     9541 cgatcctggc aatgaatatg gaaagagttt ttacccagat cgataccaat gagcgcaatg
     9601 ttttccatga tagttctccg aatgaaagcc tgtcctcagc atagtagggg tcggttccgg
     9661 ctgagggcga aatgacaccc taagcgttag ctctgtgtcg ttgcacgatg tcagcgacgg
     9721 tattcttgct gataccgagc tcgcgtgcga tccagcgata gctgcgtccc tcggccctca
     9781 tcgcaaccac cttaggcaaa agtcggtctg attttggtcg cactccggcc tgacgaccaa
     9841 gcctcttacc acgtgccttc gcaacagcaa ggcctgactt gacccgctcg ctgatgagat
     9901 cccgctcaaa ctccgcaatg ccggaaagaa acgtcgccag cattcgtcca tacggcgacg
     9961 aaagatcgaa cgccattcca ttcatggcta tcacggaaac cttccagttc tccagttcac
    10021 gtagcgtatt gagcagatcg agcgtcgagc gcccccaccg ggaaagctca gtgaccagga
    10081 ttgcatcaat ttgtctggac tgggcaagcg ccaggacttt ctttcgctcg gcccggtcga
    10141 gtttagttcc tgaacctgtt tccttaaata ttcccaccac gtcgtagccg gcacggccgg
    10201 cgaaggctcg cagatcaaat tcctggcgtt cacaagactg atccgctgtt gaaacccggc
    10261 agtaaatggc ggcacgatgt cccaattgaa ccctcctgga tttttgtatc ggaacgccct
    10321 gatttatatg ggctggctgt tgtccaaaac agactatact tcaaaaggga cgaatttgta
    10381 tgtcacgacg ccatattttc accgaacggc agcgagcagc gctgttcgat ctgcccacgg
    10441 acgaactgtc gctactgaag ttctacacgc tgggcgatga tgacctggaa aacattaggc
    10501 agcgccgcag accggaaaac aggattggct ttgccctgca actttgtgcc ttacgatatc
    10561 cgggccgtgc actggctcct ggtgagatga tcccgcgtga agtcctttcc ttcgtcggtg
    10621 ctcagcttgg agttccggct gatgcgcttc tcacttatgc cacacggcgc caaacccgtc
    10681 agcagcacat ggacacgctg cgcgaaattt acggctacaa gaccttcacg ggccgtggtg
    10741 cccgtgatct gcgggagtgg actttcggcc aggccgaaga tgccagatca aacgaggatc
    10801 ttgctcatcg ttttattgtg cggtgtcggg aaacttccac cattctgccc gcagtatcga
    10861 caatcgagcg cttgtgcgcg gatgctctgg tcgccgctga gcggcggatt gaaacgcgga
    10921 ttgcggaaaa tttaacagcg gatgttcgcg atcacctgga caaacttctg agtgaaatgc
    10981 tcgccggcaa tatcagtcgt ttcatctggc ttcgcaactt cgaggttggt aacaactcgg
    11041 ctgctgctaa ccgtttgctc gacaggctcg aatttctgcg taccctgaat atcaatcata
    11101 gtgctttggc cagcatacct gcccatcgca ttgcccggct gcgtcggcag ggtgaacgct
    11161 acttcaccga cggtttgcgt gacatcactt cggaccgccg ctgggcgatc cttgccgtct
    11221 gtgttgtgga gtgggaagcg gcgattgctg atgccatagt cgaaacccat gacaggatcg
    11281 taggaaaaac ctggcgggaa gcgaagcgcc agcatgacga aacaatttcc ggctctaaag
    11341 ccacactcac ggatacgatc cgtaccttca ccgcgctggg agcttcgttg cttgaggccc
    11401 gcagtgacgg aaccccgctg gagatggctg tcgccagttc ggttgcatgg gaccggctcg
    11461 ctcaactggt agcgacaggg actcaactca gcaacacgct agccgatgag cctcttgcat
    11521 atgtcgggca gggataccat cgctttcgtc gttatgcgcc ccgcatgttg cgctgtctga
    11581 agctcgaagc cgcgccggtc gccggaccat tggtagcagc agctttgtcg atcggagaga
    11641 tgaaaggtgt tgcatcgcca gaaaggcgtt tcctgcggcc cagctccaaa tggaaccgtc
    11701 atttacgagc tcaggaaaaa ggagataccc gtctttggga agtggcggta ctctttcacc
    11761 tccgggatgc ttttcgttcc ggagatgtct ggctcgctca ttcgcgccgc tatggtgacc
    11821 tcaagcaggt actggtgccg atgatcgcgg cgcaggaaaa tgcaaaactg gccgtgcctt
    11881 ccaacccaca ggattggctg gcagacagaa aggcgcgact cacgatcgct cttaagcggc
    11941 tggcccgggc tgcccgtaac ggcactattc cgcacggtag catagaagat ggaacgttgc
    12001 ggatcgacag gttgacagca gacgtgccgg atggtgccga ggcactcata ctggatctgt
    12061 atcgccgaat gccgtccgtt cggattaccg acatgctgct tgaagttgat gcagcccttg
    12121 gtttcacaga tgcgtttacc catctgagaa ccggggctcc atgtcgcgac cggatcggtc
    12181 tgctcaacgt cctgctcgct gaagggctca atctgggcct gcgtaagatg gcggaagcta
    12241 caaacacgca tgattactgg cagctctcac gccttgcccg ctggcatgtt gaaagcgaag
    12301 ccatgaacca ggcattggca attgtggtgg ccgcgcaggg taaactgccg atgtcacgcg
    12361 tctgggggat gggcacgtca gcatcgagcg atggtcagtt tttcccgaca gcgcggcatg
    12421 gcgaagccat gaacatggtc aatgccaaat atggttctgt tcccggcctc aaagcgtata
    12481 ctcacgtaag cgaccagttc gcgccattcg cttgtcagtc gatcccggcg accgtgagcg
    12541 aggcaccgta tattctcgat ggactactga tgaacgaggt cggtcgccat gttcgcgaac
    12601 agtatgccga tacagcagga ttcaccgacc atttgttcgg agccagtagc ctgctcggct
    12661 acaatctcgt tctgcgaatc agggatctgc catcgaagcg gttgtacgta tttaatcccg
    12721 atacgacccc cagggagtta cgcaagttgg taggtggaaa agcccgggag gatcttatcg
    12781 ttgcgaactg gcct
//
