LOCUS       CP024520                1916 bp    DNA     circular BCT 06-NOV-2017
DEFINITION  Klebsiella pneumoniae strain KSB1_10J plasmid unnamed5, complete
            sequence.
ACCESSION   CP024520
VERSION     CP024520.1
DBLINK      BioProject: PRJNA351909
            BioSample: SAMEA3357381
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: KSB1_10J
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 117x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/30/2017 11:11:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,677
            CDS (total)                       :: 5,559
            Genes (coding)                    :: 5,388
            CDS (coding)                      :: 5,388
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 77 of 171
            Pseudo Genes (incomplete)         :: 87 of 171
            Pseudo Genes (internal stop)      :: 43 of 171
            Pseudo Genes (multiple problems)  :: 32 of 171
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB1_10J"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
     gene            63..176
                     /locus_tag="CTI58_28365"
     CDS             63..176
                     /locus_tag="CTI58_28365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="ATR18815.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPERHDLSPLAAATGN"
                     /name="replication initiation protein"
     gene            201..417
                     /locus_tag="CTI58_28370"
                     /pseudo=""
     CDS             201..417
                     /locus_tag="CTI58_28370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255616.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            482..784
                     /locus_tag="CTI58_28375"
     CDS             482..784
                     /locus_tag="CTI58_28375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="ATR18813.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGAHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     gene            complement(1043..1279)
                     /locus_tag="CTI58_28380"
     CDS             complement(1043..1279)
                     /locus_tag="CTI58_28380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATR18814.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            1505..1621
                     /locus_tag="CTI58_28385"
                     /pseudo=""
     CDS             1505..1621
                     /locus_tag="CTI58_28385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557753.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     Typing          complement(1445..1618)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          2..171
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.567"
                     /coverage="90.0"
                     /evalue="1.54e-65"
     Typing          1597..1916
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.547"
                     /coverage="81.0"
                     /evalue="7.36e-127"
ORIGIN
        1 tttatctcat tccacgcctg acactcagtt ccgcgaaggc agttcgctcc tggatggact
       61 gtatgcacga accccccgtt cagtccgact gctgcgcctt atccggtaac tatcatcttg
      121 agtccaaccc ggaaagacac gacttatcgc cactggcagc agccactggt aactgattta
      181 gaggagttag tcttgaagtt atgcgcctgt taaggctaaa ctgaaagaac agatttggtt
      241 actgcgctcc tcgtaagcca gttaccacgg ttcaaagagt tgctgctctg agaaccttcg
      301 aaaaaccgcc ttgcagggcg gttttttcgt ttacagagca agagattacg acgccacaga
      361 aaggatctca agaagatcct tcaccgtaat ggaaatctat ccaatccgag cacataggat
      421 acacatcgtt ataaaacatc ggatattatg atcccaatta aaataagctc gaggataaaa
      481 aatgagttca atggtttact gccgaggatg tggcaaggaa atacacgaaa cagcaaagtc
      541 atgcccgcac tgcggagcca caaacgcctc ttcaggttca ggtgaaaaaa gtagaatcgc
      601 agccgcgcta ttagcattct tccttggtgg atttggcgca cataaatttt acttaggaaa
      661 aataggacag gggttcctgt atctaatttt ttgctggaca ttcatacccg cgatcatcgc
      721 gtttattgag tttattattt atctatgcga ttccgatgaa aaattcgcac gaaaatatgg
      781 ctaactcata gcaaaaaaaa gccccctaag ggcttttttt gtacctgacg gtcagaccga
      841 ttaaacataa catcccttgc ccgcaccccc gcaggcgccc tcagcgcaat cctgctgcat
      901 cagggccgtt tccagcacct ttcctcacca gtatcccgga aaaacgcccc tgatgatttc
      961 tgcgcgactg catggctatg cacgcaggtg aatggcaggt gatttcgcag cgtcagcacc
     1021 actaaaccgc caaaagccaa aatcagcggc gcggcgcgcg cggacgtgac caggcaaaat
     1081 cggactgctg ccgctcatcc tcacgtaatg cacgctggcg gtcggcgtaa cgctgcaact
     1141 caatttgcgc acgcgctctg ttcaggacga tgtgccggtt aggctgattt agctttttac
     1201 ctgacgctct ttcagctgcg gcaacagcat catcaagaaa ttttttctct gcaggcgtca
     1261 agtttgtcag tctggccata acgttctcca gtcagaataa atcagcacag tctgccagac
     1321 acagtagaaa cgatgaagcg ccagcgacgg tgaaaggcag tcagaaacgg tgaacgggag
     1381 cggattttaa tccggtgccg tgaggcgttg gacaagccgc aggcgcgtca ttagctttta
     1441 gcgggtttcg gggcgcagcc ctgaaccagt cacgtagcgc tagcggagtg tatactggct
     1501 gactatgttg gcacggatgt ggaaatcagt gaagtgcttc atgtagcagg agaaaaaagg
     1561 cggcaccagc gcgtcagcag gatatgtagt acaggatata ttccgcttcc tcgctcactg
     1621 actcgctgcg ctcggtcgtt cggctgcggc gagcggtgcc ggcttacagg cggggcggaa
     1681 atttcctggg agataccagg aaggagctta acagggaaga gataaggccg cggcgaagcc
     1741 gtttttccat aggctccgcc cccctgacaa acatcatgaa atctgacgct caaatcagtg
     1801 gtggcgaaac ccgatgcggc ttaaagatac caggcgtttc cccctggtgg ctccctcgtg
     1861 ctcccccgca ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcg
//
