LOCUS       CP024519                3223 bp    DNA     circular BCT 06-NOV-2017
DEFINITION  Klebsiella pneumoniae strain KSB1_10J plasmid unnamed4, complete
            sequence.
ACCESSION   CP024519
VERSION     CP024519.1
DBLINK      BioProject: PRJNA351909
            BioSample: SAMEA3357381
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: KSB1_10J
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 117x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/30/2017 11:11:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,677
            CDS (total)                       :: 5,559
            Genes (coding)                    :: 5,388
            CDS (coding)                      :: 5,388
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 77 of 171
            Pseudo Genes (incomplete)         :: 87 of 171
            Pseudo Genes (internal stop)      :: 43 of 171
            Pseudo Genes (multiple problems)  :: 32 of 171
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB1_10J"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
     gene            25..265
                     /locus_tag="CTI58_28340"
                     /pseudo=""
     CDS             25..265
                     /locus_tag="CTI58_28340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            440..784
                     /locus_tag="CTI58_28345"
     CDS             440..784
                     /locus_tag="CTI58_28345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="ATR18809.1"
                     /translation="MADKRSKMLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPAR
                     AGKLPSISPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            774..2267
                     /locus_tag="CTI58_28350"
     CDS             774..2267
                     /locus_tag="CTI58_28350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="ATR18812.1"
                     /translation="MIVKFHPRGRGGGGGPVDYLLGKDRQRDGASVLQGKPDEVRELID
                     ASPYAKKYTSGVLSFAEQDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHRDKGRLE
                     LNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRVLVSPSAL
                     PEAKQEAAQAITSGLLALASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGRN
                     IRLKGAIYEQSFNAGEGLRAEIESAAADYRRDAESRIQRAREVCQSGTERKREENQRRH
                     PRPRPEAVLSHEPAHERDAAHGQPDVADHRSGLRAADSVERGHSVVAGAADTRELYDHP
                     GAEEHAGHAVREEQRRTALDLRRAETPLREGEPGSGTVRRGLELDDTGGEIAHDGAGKT
                     VAERIRAATAGLLEKAGRVGERLRGMADDVWSYSTGERGAERARHGLEQAGAEFERAAA
                     PVVERLNAIETHRQQERAVQHQKALELERSQWQHHGPSL"
                     /name="nuclease"
     gene            2334..2666
                     /locus_tag="CTI58_28355"
     CDS             2334..2666
                     /locus_tag="CTI58_28355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005969762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug SMR transporter"
                     /protein_id="ATR18810.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="multidrug SMR transporter"
     gene            complement(2875..3045)
                     /locus_tag="CTI58_28360"
     CDS             complement(2875..3045)
                     /locus_tag="CTI58_28360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="ATR18811.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="replication initiation protein"
     ARG             2335..2666
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          174..365
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          complement(2..191)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.513"
                     /coverage="93.0"
                     /evalue="3.33e-30"
     Typing          complement(2701..3223)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.143"
                     /coverage="99.0"
                     /evalue="8.31e-121"
     Typing          775..2264
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctgatttga gcgtcatttt cgtgatactc gtcagggggg cggagcctat ggaaaaacgg
       61 caactccgca tccttttctt ggcctttcct gcttcgagcg gcatttgctt atcccgtaat
      121 ctgtgagctg gtaacgcccg ccgcagtctc acggcaggag cgtagcgacc gagtgagcga
      181 ggaggcgtca tttcatcacc acctgcattt tgcacttacg cccggcagcc gcatttttcc
      241 cctgtcacat gaagcacttt cataaattgc cactccgtca taacatgttt agccagtata
      301 cactccgcta gcgctacgtg actggttcag ggctgcgccc cgaaacccgc taaaaccact
      361 gccgcgcctg cggcttgtcc aacaccgtgc cgaccgggaa aagatttccc gtccgtcccg
      421 gcgttatcag gaggccggat tggcagacaa acgcagcaaa atgctcacca tgtgggtgac
      481 tgaggacgag caccggcgtc tgctggagcg ctgcgagggt aagcaactcg cggcctggat
      541 gcggcagacg tgcctggacg agaagcccgc acgcgccggc aaacttccgt cgatctcgcc
      601 ggcgctgctt cgtcagcttg ccggcatggg gaacaacctt aaccagattg cccgccaggt
      661 taacgccggt ggtggaagcg gacacgaccg cgtgcagatt gtcgccgcgc tgatggccat
      721 cgatgccgga ctcgagcggt tgcggcatgc cgtactggaa aagggtgctg atgatgatcg
      781 ttaagtttca tcctcggggg cgcggcggcg gcggcgggcc ggtcgattat ctgctgggta
      841 aagatcgcca gcgcgacggc gccagcgttc tgcaggggaa gccggatgaa gtccgggagc
      901 ttatcgacgc ctcgccctac gccaaaaagt acacttccgg cgttctgtct tttgccgaac
      961 aggatttacc gcccggccag cgcgaaaagc tgatggcgag tttcgagcgg gttctgatgc
     1021 ccggactcga taaagaccag tacagcgtgc tgtgggttga gcaccgggac aaggggcggc
     1081 tggagctgaa cttcctgatc ccgaacacgg agcttctgac cggcaagcgg ctacagccgt
     1141 attacgaccg cgccgaccgt ccgcgcatcg atgcctggca gaccatagtg aacggcaggc
     1201 tggggctgca tgacccgaac gcgccggaga accggcgggt gctggtctcg ccttccgcgc
     1261 tgccggaagc gaagcaggaa gccgcccagg cgattacgag cggcctgctt gcccttgcct
     1321 catccgggga gctgaaaacg cgtcaggacg tcactgaggc gctggaaagc gcaggttttg
     1381 aggtggtgcg caccacaaaa agcagcatca gcattgccga cccggacggg gggcgaaaca
     1441 tccgactgaa gggagccatc tatgaacagt cttttaacgc tggcgaagga cttagagcag
     1501 aaatcgaaag cgcagcagca gactaccggc gagatgctga aagccgcatt cagcgagcac
     1561 gagaagtctg tcagagcgga actgagcgaa agcgagaaga gaatcagcgc cgccatcctc
     1621 gaccacgacc ggaagctgtc ctcagccatg agccagcgca cgaaagggat gctgcgcatg
     1681 gtcagccaga cgtggctgac catcgttctg gtctccgcgc tgctgatagc gtcgagcgcg
     1741 ggcattctgt ggtggcaggg gcagcagata ctcgagaact atacgaccat ccgggagcag
     1801 aagagcacgc aggccatgct gtcagagagg aacagcggcg tacagctctc gacctgcggc
     1861 gagcagagac gccgctgcgt gagggtgaac ccggaagcgg gacagttcgg agaggactcg
     1921 agctggatga tactggcggg gaaatagcac atgacggagc tggaaaaaca gttgctgagc
     1981 gcattagagc agctacagca ggactactcg aaaaggctgg acgagtggga gaacgccttc
     2041 gcggaatggc ggacgatgtc tggtcttatt caacgggaga acgcggcgct gaacgagcgc
     2101 gtcacggtct tgagcaggca ggtgcagagt ttgagcgagc agctgcgccg gttgtcgaaa
     2161 ggctgaacgc gatagaaact caccggcagc aggagcgggc ggtgcagcac cagaaagcgc
     2221 tggagctgga acgatcgcag tggcagcatc atggtccttc gctatgataa gccgggttct
     2281 attaattagt tgttatgtaa agatatttaa atttttcaac atgaggtatt tttatgaata
     2341 catatatttt cctgggaggg gctattgtgg ctgaagtcat aggcaccacc tttatgaaat
     2401 attcagaagg atttacacgc ctgtggccgt ctgtaggaac tgcaataagt tattgcgctg
     2461 ccttttatct gctttcccaa acattggaaa atatacctac cggtattgct tacgcaattt
     2521 ggtctggtgc gggaattgta ctaataagcc tagctggttg gttggttagc ggtcaaaaac
     2581 ttgattggcc cgcaattgca ggaatgttcc ttatttgcgt tggagttttg gttattaata
     2641 ttttttcaaa atcttctgcg cattaacttt ttaccgaagc atattggcca aagcaaaagg
     2701 atttccttga gatcctttct ttctgcgcgt aatctttttc cctgtaaaca aaaaaaccac
     2761 cgctaccaac ggtggttcgt ttgccggaat tcagagctac caactctttg ttccgatggt
     2821 aactggcttt cacggagcgc agatacctaa actgtccttt cagtgtagcc gcactcaggc
     2881 tttcacttca agaactctgt aggcattctc tacaatctct gctccgtgaa cccagttacc
     2941 aatggctacc gccactggcg atttgtcgtg tctttccggg ttggactcaa gttgatagtt
     3001 accggaacgg gcgtggtagt cggactgaac ggggggttcg tgcatacagt ccatcctgga
     3061 gcgaactgcc tacccggaac cgagtttcag gcgtggaatg agaaaccgcg gccataacag
     3121 cggaatgaca ccggtaaacc gaaaggcagg aatgcggggg agcacgaggg agccaccagg
     3181 gggaaacgcc tggtatcttt aagccgcatc gggtttcgcc acc
//
