LOCUS       CP024518                4064 bp    DNA     circular BCT 06-NOV-2017
DEFINITION  Klebsiella pneumoniae strain KSB1_10J plasmid unnamed3, complete
            sequence.
ACCESSION   CP024518
VERSION     CP024518.1
DBLINK      BioProject: PRJNA351909
            BioSample: SAMEA3357381
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: KSB1_10J
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 117x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/30/2017 11:11:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,677
            CDS (total)                       :: 5,559
            Genes (coding)                    :: 5,388
            CDS (coding)                      :: 5,388
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 77 of 171
            Pseudo Genes (incomplete)         :: 87 of 171
            Pseudo Genes (internal stop)      :: 43 of 171
            Pseudo Genes (multiple problems)  :: 32 of 171
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB1_10J"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
     gene            complement(join(2867..4064,1..62))
                     /locus_tag="CTI58_28315"
     CDS             complement(join(2867..4064,1..62))
                     /locus_tag="CTI58_28315"
                     /inference="COORDINATES: protein motif:HMM:PF03389.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobA"
                     /protein_id="ATR18808.1"
                     /translation="MALYRLEMQNVSRSDGVSSVAKAAYRHRSVMIDERTGEIHGEKSA
                     NRDDLVYAEILAPEGTPDFLIKSSNDLWNYVEKTEKRKDARTAKEFKITLPCELTNEQN
                     IKLLKEYLTKNFVSKGIICDFVIHNDKDNKNPHAHVMITTREITLSGFGKKVRSWDEEK
                     TLHDWRKQWAKVQNQHLKNAGLKSRVSHRTLEEQKNIMIDLAKKAEDAKDFDKSILYLA
                     KAIELDRPPMQNLSRKKWRTKEGQEQRKRDQAIRDRARADARAFRLLRGVAVVDVASFV
                     VHPLEKKEQSKNNDFSEKKSWWRSTVNFIKNIKAKFSTKKDIKNDVHASDDLMTDPVTG
                     LPITKAKWQEMSDRANVRTPQPAISYQVKQSDGEGLKTAQNALQDVLDVSEGKSLKVKS
                     VPVPGSRKRRVVEEDSSDNG"
                     /name="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobA"
     gene            358..753
                     /locus_tag="CTI58_28320"
     CDS             358..753
                     /locus_tag="CTI58_28320"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATR18804.1"
                     /translation="MNKKEIEKRLLIFTSEIDIEDIKNDFINDEVNEDVFNRCLKLYYA
                     IDDAIYNNKKSLFEALIYLINKDRENKDHDEPIITDYDLVYLTLNNYINIYELKKLFLL
                     QKNINDFDFKSMISEIEKIKREVEYGK"
                     /name="hypothetical protein"
     gene            743..1021
                     /locus_tag="CTI58_28325"
     CDS             743..1021
                     /locus_tag="CTI58_28325"
                     /inference="COORDINATES: protein motif:HMM:PF06412.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATR18805.1"
                     /translation="MGSKKTLEQRMALAQDRLSKLKKEASQKERKAREHKKFTVGGEVT
                     RVFGLDYDLALVVGFLEHFNKLSDEQKQALKNNGERLLKSRLENADK"
                     /name="hypothetical protein"
     gene            complement(1435..1704)
                     /locus_tag="CTI58_28330"
     CDS             complement(1435..1704)
                     /locus_tag="CTI58_28330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="ATR18806.1"
                     /translation="MKGNEIRKGLTQKEMAKKFNLSLSTVKKYTSQPREHYESEAENRR
                     KTAYTLHHKKGLKWREVAEKMNTTENAVKALAKRYKQIDQENRG"
                     /name="plasmid replication protein"
     gene            complement(1701..2558)
                     /locus_tag="CTI58_28335"
     CDS             complement(1701..2558)
                     /locus_tag="CTI58_28335"
                     /inference="COORDINATES: protein motif:HMM:PF08708.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="ATR18807.1"
                     /translation="MILQKEYSRSDFINQIISRPFCTDDFKRDGIYRTNKRRALNSVYI
                     EHNNDSFINSIVFDIDSDTAAIAWQDADIPKPNFITQNPANGHAHLFYALSSPVCITEN
                     ARRKPQKLLKGVIEGLTERLGADPCYTGKITKNPLNPRWRTFWNEQPRFELNYLCEFID
                     TNKRVKKEVRKSIVAEGRNTALFDSLRFYAYSIIFKYQKNDDFCGFISALQEEAENIND
                     SFEDQLSFKEINHTIRSISSWVWGTFDSDTFSEIQSNRAKKAKGKTKTNTKAKKLIEFI
                     GVKK"
                     /name="replication protein"
     Typing          complement(2871..4036)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcaactgagc tgacaccatc cgaacgacta acgttctgca tttctaatct atataaagcc
       61 atataattat atcctcatga gaacccctat tttttctttt gggtttgggt tttagttttt
      121 cgtaagaaaa acaggtttta gccgtcggca gaccccggca ttgtttacaa tgcgtaaccg
      181 ggcattcctt agcttttatt tttcgctaac gctcaaacta aaagaggaat gaaaaacaaa
      241 ctagcttttc tttcactgat atatataaat taactctgat taaattaaag tcaaatttga
      301 caatatttaa aagttacgat attgaataaa aaacaagcat tatggattgg tggtaaaatg
      361 aataaaaaag aaatagaaaa aaggctattg atttttacaa gcgagatcga cattgaagat
      421 attaaaaacg atttcatcaa tgacgaagtt aatgaagatg tttttaatag gtgtttaaag
      481 ctgtattacg ctatcgatga tgcaatctat aacaataaaa aatcattgtt tgaagcgttg
      541 atatatttga taaataaaga tcgtgaaaat aaagatcatg atgaaccaat tataactgat
      601 tatgatcttg tgtatttaac actaaataat tacataaata tctatgaact gaaaaagttg
      661 tttttattac aaaaaaatat caatgatttt gattttaaaa gtatgatttc agagatagaa
      721 aaaattaaaa gagaggttga gtatgggaag taaaaaaacg ttagagcaaa gaatggcact
      781 tgcacaagat agattatcta aacttaaaaa agaagcatcg cagaaagaaa gaaaagctag
      841 agaacataaa aaatttactg ttggcggtga agttacaagg gtgtttggtt tagattacga
      901 tctagctctt gtagtgggtt ttcttgaaca ctttaacaag ttaagcgatg agcaaaaaca
      961 agcgttaaaa aacaatggtg aaagattgct aaaatcacgt ctagaaaacg ctgacaagtg
     1021 acagatttct aatctgacac ttggaagggt tgaacaagca agaacccgcc gctggcgggg
     1081 cttgtgcgtt agtcttcacc gtagcccggc tttgtgatat gtttaaaaaa catatcatcc
     1141 gggagagtaa aaaatgtctc aaaaaaaaga gcaaaaaaca gcagaaaaac caactgcagg
     1201 gccacttcgt aaacacagat ctagagctga aaaaatagac gatttaaaaa agcagattag
     1261 cgaactaaga aaagcagaaa gagaagaacg acaacgagca agagacgaaa aattaattat
     1321 tcttgcaaaa gctgtttatg aaaaggcagg aaaaccaaaa gatattgaag cattaattga
     1381 tttcgtaaag tctgaagatt tcaagattga atataaataa agctaagggg cattttagcc
     1441 cctgttttct tggtcgattt gtttgtatct tttggctaat gctttaacgg cattttctgt
     1501 tgtattcatt ttttcagcta cttcacgcca ttttaagcct tttttatggt gtaacgtata
     1561 agctgttttt cttctgtttt ctgcttctga ttcataatgt tctcttggtt gagaagtgta
     1621 tttttttaca gtgcttaaag aaagattgaa ctttttagcc atctcttttt gtgttaagcc
     1681 ctttcttatc tcgttgcctt tcattttttt actcctataa attctataag tttttttgct
     1741 tttgtattgg ttttagtttt acctttcgct ttctttgctc tgttgctttg tatttcgctg
     1801 aaagtatcac tatcaaaagt cccccatacc caagatgaaa tgcttcttat tgtgtggttt
     1861 atttctttaa agcttagttg atcttcgaat gaatcgttta tgttttcagc ttcttcttgt
     1921 aatgctgaaa tgaaaccgca gaaatcatca tttttttgat atttaaaaat tatgctgtaa
     1981 gcgtaaaaac gcagactatc aaataacgct gtattccttc cctctgcaac tattgatttt
     2041 cttacttctt tttttactct tttatttgta tctatgaact cacatagata gttcaattca
     2101 aatcttggtt gttcgttcca aaatgtacgc catctcggat tgagagggtt ttttgttatc
     2161 ttgcccgtat aacaaggatc tgcacctaat ctttcggtga gtccttctat aacgcctttt
     2221 aataactttt gtggctttct tctggcgttt tctgttatac aaacaggaga tgataaggca
     2281 taaaataaat gagcgtgacc attagccgga ttttgagtta taaagttagg cttcggtata
     2341 tctgcatctt gccatgcaat agccgctgta tcgctgtcaa tatcaaacac aatagagtta
     2401 atgaagctat cgttgttatg ctcgatataa acgctgttta aagctcgtct tttgtttgtt
     2461 ctataaatac catctctttt gaagtcgtca gtgcaaaatg gtcttgatat aatttgattt
     2521 ataaaatcac ttctgctata ttctttttgt agtatcattt tttaatacaa tccttttatt
     2581 gtagatccct gattttctcc tgtcgccaaa cattcggaaa tcagggattt ttgtttttat
     2641 attactgttc taacataacc tttcatggtg tcaagtagtc accactaaaa aagtgtcaag
     2701 tggtcatttt agccatatca ggtaataatg ggtctggggg tatttttgcg aagcacaggc
     2761 cgaaggcttc aaatcatcac aagttaagct atagcctgat aggtacaggc cgtaggctat
     2821 gagcgaagcc ctgtaatctt atgtaggatc tggctgtgct ggttagctaa ccgttgtcac
     2881 tgctgtcttc ctcgacaact cgacgcttac gagaacccgg aaccggcaca gacttcacct
     2941 ttaacgattt accctctgat acgtctaaaa cgtcctgtaa cgcattctga gccgttttta
     3001 gcccttcccc gtccgattgc ttaacctgat atgagatcgc aggctgtgga gtccttacgt
     3061 ttgccctgtc actcatctct tgccatttcg ctttggtgat tggtaaaccc gtcaccggat
     3121 cagtcattaa atcgtctgat gcatggacat cgtttttaat atctttttta gttgagaact
     3181 tagctttaat attttttata aaattaacag tagaacgcca ccaagatttt ttttctgaaa
     3241 agtcattatt ttttgactgc tcttttttct ctaacggatg aaccacgaat gaagcaacat
     3301 caacaactgc aacacctctc aacaacctga aagccctagc gtcggctctc gccctgtctc
     3361 tgatagcttg gtcacgtttg cgttgttctt gaccttcttt tgttctccat tttttacgtg
     3421 ataagttctg cattggtggg cgatctagtt caatagcttt tgctagatat aaaatagact
     3481 tgtcaaaatc ttttgcatct tctgcttttt tagctaaatc gatcattata tttttttgtt
     3541 cttctagtgt tctatgactg acgcgagatt ttaagcctgc atttttcaaa tgctgatttt
     3601 gaacttttgc ccattgttta cgccaatcat gaagggtttt ttcttcatcc catgacctta
     3661 cttttttacc aaatcctgaa agcgttattt ctcttgtcgt aatcatcaca tgagcgtgcg
     3721 ggtttttatt gtctttgtcg ttatgtataa caaagtcgca aatgatgcct ttactaacaa
     3781 agttttttgt taagtattct ttcaatagtt ttatattttg ttcattggta agttcacaag
     3841 gcaaagttat tttaaattct tttgctgttc ttgcgtcctt tctcttctct gttttttcaa
     3901 catagttcca tagatcattg cttgatttaa tcaaaaaatc tggcgttcct tctggtgcca
     3961 atatttcagc ataaaccaga tcgtcacgat tagctgattt ctcgccgtgt atttctccgg
     4021 ttcgttcatc tatcatgacg gaacgatgac gataagcagc tttt
//
