LOCUS       CP024514                3223 bp    DNA     circular BCT 06-NOV-2017
DEFINITION  Klebsiella pneumoniae strain KSB2_1B plasmid unnamed8, complete
            sequence.
ACCESSION   CP024514
VERSION     CP024514.1
DBLINK      BioProject: PRJNA351909
            BioSample: SAMEA3357405
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: KSB2_1B
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/30/2017 11:04:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,094
            CDS (total)                       :: 5,976
            Genes (coding)                    :: 5,724
            CDS (coding)                      :: 5,724
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 252
            Pseudo Genes (ambiguous residues) :: 0 of 252
            Pseudo Genes (frameshifted)       :: 99 of 252
            Pseudo Genes (incomplete)         :: 154 of 252
            Pseudo Genes (internal stop)      :: 63 of 252
            Pseudo Genes (multiple problems)  :: 55 of 252
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB2_1B"
                     /db_xref="taxon:573"
                     /plasmid="unnamed8"
     gene            225..440
                     /locus_tag="CTI57_30435"
     CDS             225..440
                     /locus_tag="CTI57_30435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="ATR13424.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="mobilization protein"
     gene            608..940
                     /locus_tag="CTI57_30440"
     CDS             608..940
                     /locus_tag="CTI57_30440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005969762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug SMR transporter"
                     /protein_id="ATR13425.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="multidrug SMR transporter"
     gene            complement(1149..1319)
                     /locus_tag="CTI57_30445"
     CDS             complement(1149..1319)
                     /locus_tag="CTI57_30445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="ATR13426.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="replication initiation protein"
     gene            complement(1344..1556)
                     /locus_tag="CTI57_30450"
                     /pseudo=""
     CDS             complement(1344..1556)
                     /locus_tag="CTI57_30450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001692121.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing start; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            1522..1762
                     /locus_tag="CTI57_30455"
                     /pseudo=""
     CDS             1522..1762
                     /locus_tag="CTI57_30455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            1937..2281
                     /locus_tag="CTI57_30460"
     CDS             1937..2281
                     /locus_tag="CTI57_30460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="ATR13427.1"
                     /translation="MADKRSKMLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPAR
                     AGKLPSISPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            2220..2984
                     /locus_tag="CTI57_30465"
                     /pseudo=""
     CDS             2220..2984
                     /locus_tag="CTI57_30465"
                     /inference="COORDINATES: protein motif:HMM:PF03432.12"
                     /note="incomplete; partial on complete genome; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /name="nuclease"
     gene            2959..3105
                     /locus_tag="CTI57_30470"
                     /pseudo=""
     CDS             2959..3105
                     /locus_tag="CTI57_30470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063820.1"
                     /note="incomplete; partial on complete genome; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /name="mobilization protein"
     ARG             609..940
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          1671..1862
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          complement(1208..1688)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          3..538
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.083"
                     /coverage="99.0"
                     /evalue="8.68e-87"
     Typing          2269..3221
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.912"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tagcgtcgag cgcgggcatt ctgtggtggc aggggcagca gatactcgag aactatacga
       61 ccatccggga gcagaagagc acgcaggcca tgctgtcaga gaggaacagc ggcgtacagc
      121 tctcgacctg cggcgagcag agacgccgct gcgtgagggt gaacccggaa gcgggacagt
      181 tcggagagga ctcgagctgg atgatactgg cggggaaata gcacatgacg gagctggaaa
      241 aacagttgct gagcgcatta gagcagctac agcaggacta ctcgaaaagg ctggacgagt
      301 gggagaacgc cttcgcggaa tggcggacga tgtctggtct tattcaacgg gagaacgcgg
      361 cgctgaacga gcgcgtcacg gtcttgagca ggcaggtgca gagtttgagc gagcagctgc
      421 gccggttgtc gaaaggctga acgcgataga aactcaccgg cagcaggagc gggcggtgca
      481 gcaccagaaa gcgctggagc tggaacgatc gcagtggcag catcatggtc cttcgctatg
      541 ataagccggg ttctattaat tagttgttat gtaaagatat ttaaattttt caacatgagg
      601 tatttttatg aatacatata ttttcctggg aggggctatt gtggctgaag tcataggcac
      661 cacctttatg aaatattcag aaggatttac acgcctgtgg ccgtctgtag gaactgcaat
      721 aagttattgc gctgcctttt atctgctttc ccaaacattg gaaaatatac ctaccggtat
      781 tgcttacgca atttggtctg gtgcgggaat tgtactaata agcctagctg gttggttggt
      841 tagcggtcaa aaacttgatt ggcccgcaat tgcaggaatg ttccttattt gcgttggagt
      901 tttggttatt aatatttttt caaaatcttc tgcgcattaa ctttttaccg aagcatattg
      961 gccaaagcaa aaggatttcc ttgagatcct ttctttctgc gcgtaatctt tttccctgta
     1021 aacaaaaaaa ccaccgctac caacggtggt tcgtttgccg gaattcagag ctaccaactc
     1081 tttgttccga tggtaactgg ctttcacgga gcgcagatac ctaaactgtc ctttcagtgt
     1141 agccgcactc aggctttcac ttcaagaact ctgtaggcat tctctacaat ctctgctccg
     1201 tgaacccagt taccaatggc taccgccact ggcgatttgt cgtgtctttc cgggttggac
     1261 tcaagttgat agttaccgga acgggcgtgg tagtcggact gaacgggggg ttcgtgcata
     1321 cagtccatcc tggagcgaac tgcctacccg gaaccgagtt tcaggcgtgg aatgagaaac
     1381 cgcggccata acagcggaat gacaccggta aaccgaaagg caggaatgcg ggggagcacg
     1441 agggagccac cagggggaaa cgcctggtat ctttaagccg catcgggttt cgccaccgct
     1501 gatttgagcg tcattttcgt gatactcgtc aggggggcgg agcctatgga aaaacggcaa
     1561 ctccgcatcc ttttcttggc ctttcctgct tcgagcggca tttgcttatc ccgtaatctg
     1621 tgagctggta acgcccgccg cagtctcacg gcaggagcgt agcgaccgag tgagcgagga
     1681 ggcgtcattt catcaccacc tgcattttgc acttacgccc ggcagccgca tttttcccct
     1741 gtcacatgaa gcactttcat aaattgccac tccgtcataa catgtttagc cagtatacac
     1801 tccgctagcg ctacgtgact ggttcagggc tgcgccccga aacccgctaa aaccactgcc
     1861 gcgcctgcgg cttgtccaac accgtgccga ccgggaaaag atttcccgtc cgtcccggcg
     1921 ttatcaggag gccggattgg cagacaaacg cagcaaaatg ctcaccatgt gggtgactga
     1981 ggacgagcac cggcgtctgc tggagcgctg cgagggtaag caactcgcgg cctggatgcg
     2041 gcagacgtgc ctggacgaga agcccgcacg cgccggcaaa cttccgtcga tctcgccggc
     2101 gctgcttcgt cagcttgccg gcatggggaa caaccttaac cagattgccc gccaggttaa
     2161 cgccggtggt ggaagcggac acgaccgcgt gcagattgtc gccgcgctga tggccatcga
     2221 tgccggactc gagcggttgc ggcatgccgt actggaaaag ggtgctgatg atgatcgtta
     2281 agtttcatcc tcgggggcgc ggcggcggcg gcgggccggt cgattatctg ctgggtaaag
     2341 atcgccagcg cgacggcgcc agcgttctgc aggggaagcc ggatgaagtc cgggagctta
     2401 tcgacgcctc gccctacgcc aaaaagtaca cttccggcgt tctgtctttt gccgaacagg
     2461 atttaccgcc cggccagcgc gaaaagctga tggcgagttt cgagcgggtt ctgatgcccg
     2521 gactcgataa agaccagtac agcgtgctgt gggttgagca ccgggacaag gggcggctgg
     2581 agctgaactt cctgatcccg aacacggagc ttctgaccgg caagcggcta cagccgtatt
     2641 acgaccgcgc cgaccgtccg cgcatcgatg cctggcagac catagtgaac ggcaggctgg
     2701 ggctgcatga cccgaacgcg ccggagaacc ggcgggtgct ggtctcgcct tccgcgctgc
     2761 cggaagcgaa gcaggaagcc gcccaggcga ttacgagcgg cctgcttgcc cttgcctcat
     2821 ccggggagct gaaaacgcgt caggacgtca ctgaggcgct ggaaagcgca ggttttgagg
     2881 tggtgcgcac cacaaaaagc agcatcagca ttgccgaccc ggacgggggg cgaaacatcc
     2941 gactgaaggg agccatctat gaacagtctt ttaacgctgg cgaaggactt agagcagaaa
     3001 tcgaaagcgc agcagcagac taccggcgag atgctgaaag ccgcattcag cgagcacgag
     3061 aagtctgtca gagcggaact gagcgaaagc gagaagagaa tcagcgccgc catcctcgac
     3121 cacgaccgga agctgtcctc agccatgagc cagcgcacga aagggatgct gcgcatggtc
     3181 agccagacgt ggctgaccat cgttctggtc tccgcgctgc tga
//
