LOCUS       CP024513                3336 bp    DNA     circular BCT 06-NOV-2017
DEFINITION  Klebsiella pneumoniae strain KSB2_1B plasmid unnamed7, complete
            sequence.
ACCESSION   CP024513
VERSION     CP024513.1
DBLINK      BioProject: PRJNA351909
            BioSample: SAMEA3357405
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: KSB2_1B
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/30/2017 11:04:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,094
            CDS (total)                       :: 5,976
            Genes (coding)                    :: 5,724
            CDS (coding)                      :: 5,724
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 252
            Pseudo Genes (ambiguous residues) :: 0 of 252
            Pseudo Genes (frameshifted)       :: 99 of 252
            Pseudo Genes (incomplete)         :: 154 of 252
            Pseudo Genes (internal stop)      :: 63 of 252
            Pseudo Genes (multiple problems)  :: 55 of 252
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB2_1B"
                     /db_xref="taxon:573"
                     /plasmid="unnamed7"
     gene            join(3272..3336,1..358)
                     /locus_tag="CTI57_30390"
     CDS             join(3272..3336,1..358)
                     /locus_tag="CTI57_30390"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="ATR13421.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="DNA-binding protein"
     gene            766..1053
                     /locus_tag="CTI57_30395"
     CDS             766..1053
                     /locus_tag="CTI57_30395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATR13418.1"
                     /translation="MIAGRRRTRFLNVLKTGFPAAPATPHGHAINCVPVHRFRIRRTPE
                     TGLTLAEWREFPRYALARILMHAVKTEPARFWRVFGWFVACFTGFPSETS"
                     /name="hypothetical protein"
     gene            complement(1106..1354)
                     /locus_tag="CTI57_30400"
                     /pseudo=""
     CDS             complement(1106..1354)
                     /locus_tag="CTI57_30400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="incomplete; partial on complete genome; missing
                     start; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            1353..1835
                     /locus_tag="CTI57_30405"
                     /pseudo=""
     CDS             1353..1835
                     /locus_tag="CTI57_30405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228432.1"
                     /note="incomplete; partial on complete genome; missing
                     start; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="klebicin B"
                     /name="klebicin B"
     gene            1837..2098
                     /locus_tag="CTI57_30410"
                     /pseudo=""
     CDS             1837..2098
                     /locus_tag="CTI57_30410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /name="bacteriocin immunity protein"
     gene            2202..2357
                     /locus_tag="CTI57_30415"
     CDS             2202..2357
                     /locus_tag="CTI57_30415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="ATR13419.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="lysis protein"
     gene            complement(2515..2676)
                     /locus_tag="CTI57_30420"
     CDS             complement(2515..2676)
                     /locus_tag="CTI57_30420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="ATR13420.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPERHDKAPLAAATGNGFRGVGLE
                     VMRLLRLN"
                     /name="replication initiation protein"
     gene            complement(2701..2958)
                     /locus_tag="CTI57_30425"
     CDS             complement(2701..2958)
                     /locus_tag="CTI57_30425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATR13423.1"
                     /translation="MEQTARRGRRQHSGRFSIGSAPLTSITEFDAQISGGETRQDYKDT
                     RRFPLMAPSCALLFLPFGLPVSFRCYGRVCLIPRLTLSSG"
                     /name="hypothetical protein"
     gene            3081..3272
                     /locus_tag="CTI57_30430"
     CDS             3081..3272
                     /locus_tag="CTI57_30430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="ATR13422.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2564..3033)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.834"
                     /coverage="92.0"
                     /evalue="2.73e-170"
ORIGIN
        1 gatgattaac agtgggaagt taagttacac ctctgttgta aagcacggga aggctattaa
       61 agttattgat acttcagagc ttattagagt gtttggttct cttgatggtg tcatcgatac
      121 tgtaaaatat gatgtaaaaa gtgacgcaga atctacaagt attaacagcg ttggtttaca
      181 tgatttacaa catagaatcg ctctacttga agctgaaaac gatggtctta aaggggctgt
      241 taaggctcga gatgagcata ttgattccct tcgtcaggct atgcagcttc tggagcacaa
      301 gcgcgagcct tcttcaccac cccattcccc gtggtggaaa ttctggaaaa aatcctgatc
      361 ctgccatgct tgagttttcc tctccggcca tcccagggag ttcctgaacc ttcccaaaaa
      421 tcgccagatc tctgaacgct cctcacgact aaaaataccc acacactacg caaaattaaa
      481 attttgcaga ttccttattt aaaacaattg gttaatgaaa aattaatgtg cagtgatgtg
      541 ttacaaagtg cagatccaag tcgtggacga ctctatggcg aatcgtttac gaattgccca
      601 cgattcatat gtgaatcacg ctttattatc atgttgttat gatgaatcga ctcttgattc
      661 aattcatcaa tttgggcgtt ttttgttcat ggtgactctt gattcaattc acgattcgaa
      721 tcgccggtga atcgtgtggt gagtcgtccg gcgacttgcc ataacgtgat tgccggccgt
      781 cgacgaaccc gattcctgaa cgtgctgaaa accggttttc cggctgcgcc ggcaacgcct
      841 cacggccacg caataaattg cgtccccgtt caccgttttc ggatcagaag aacgccggaa
      901 acaggactta ccctggctga atggcgtgaa tttccgcgct atgcacttgc gcgcatactc
      961 atgcatgccg taaaaacaga gcctgcgcgt ttctggcggg ttttcgggtg gtttgttgcc
     1021 tgttttaccg gtttcccgtc agaaacgtcc tgaggccgtt ttagcggtgc gtacaattaa
     1081 gggattatgg taaatggtga cttggttaat cataaacatc ctgggggatg gcatacacca
     1141 gttggatatc cggtgcatcg ttgagccatc gacctgattt tttcattaag tagccaccaa
     1201 tttcttcggt agtcagccgg gcatgaaaca atgcttcatc attgaaccag cagtcagggt
     1261 gatagatgag atccagtggg tttgccttga aattaatctc cagtaactgg agtgcatagc
     1321 tgtggtcaga tgcactacct tctgcttttc gtatggcgga tgacctcgat tttgacgata
     1381 ttattctggt gtttcctccg gaatccggcc taaaaccgct gtatgtgatg taccgtagcc
     1441 ctcgtaacat gcccggtaca gtgagcggca aagggcagaa cgttggcaat aactggatgg
     1501 ggggggccag taccggggat ggtgctcctg ttccttccca gattgcggat aaattacgcg
     1561 ggaagacttt cggtagtttt gattctttcc gtcgggcgtt ctggaaagcg gttgctgacg
     1621 attctgcact gagcaagcaa tttagtgaag ctgatattaa tcagatgaaa gctggaaggg
     1681 cacctactgc ggacttttta gaatcagtag ggaaacgtgt taaaattgaa ttacaccacg
     1741 aaaaagaaat atctcaagga ggggctgtta tggatgtcga taacattaag gcgctaacac
     1801 ctaaaaatca tattgagaca cataaaggaa aataaaatgg ctggtaataa aatttcagat
     1861 tttacggaag aagaatttac tgcttttgtt agtaaaataa tcgctgctga ttttccaact
     1921 gacaaagaga acgatgatgc tatttattcg ttcgcccaac taacagagca tcctgctggt
     1981 tggaacctca tcttcaagcc taagataggc gaagattctt cggcgaaagg gattgttaaa
     2041 accgtaaagg aatggcgagc tgctaacggt aagccaggtt ttaaaaaagc gtgattgaat
     2101 ttgcgggttc aagttatgag cccgctcatt tgtaactctg gatttgaaga ataattacta
     2161 gatatccatt tgcctagaat aataaaaata aaaggtgata tatgaaaaaa ttacttttcc
     2221 taggggttgt taccgtttct gtggttctgg cagcatgtca ggtcaataat gttcgagaca
     2281 ccggaggtgg ggctgtttca ccttcatcga cggtgaccgg agtcacgctt gatagcggta
     2341 cgaacggcaa tccgtaaagg atcttcttga gatcccattt ggatcgtcgt aatctcttgc
     2401 tctgtaaacg aaaaaaccgc cttggcgggc ggttttttcg aaggttcaaa cacttgcagg
     2461 tgattgaacc aaggtaactg gcttggagga gcgcagtcac caaaacttgt tctttcagtt
     2521 tagccttaac aggcgcatga cttcaagacc aactcctcta aatccgttac cagtggctgc
     2581 tgccagtggc gctttgtcgt gtctttccgg gttggactca agatgatagt taccggataa
     2641 gacgcagcag tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc
     2701 ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga
     2761 caccggtaaa ccgaatggca ggaacaggag agcgcacgag ggagccatca gggggaaacg
     2821 cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt caaattctgt
     2881 gatgcttgtc aggggggcgg agcctatgga aaaacggccg ctgtgctgcc ttctccctct
     2941 ccgagctgtc tgctctatgt cgttatgtgc gcgttgcgac cgctcgccgc agccgaacgg
     3001 caggagcgta gcgactgagt gagcgaggaa gcggaaaaga gatctgtctg acattgagca
     3061 ctgacgctct gaggcccttt atgacgaaac aacagaaaac agcgctgaac atggctaaat
     3121 tcatccagaa tcagtctctg ctcctgctgg agaagcttaa cgagcttgat ctcgatgttg
     3181 aggccgacct ttgtgagaaa ctccacgatg atgcggagca tcttttccgt acactgtcct
     3241 cgagactgga tgaattacag gatggtaatt gatggccaga gtgtctatta gtgaagcggc
     3301 acggcttgtt aaggttagcc gtcccacaat ttacaa
//
