LOCUS       CP024512                3377 bp    DNA     circular BCT 06-NOV-2017
DEFINITION  Klebsiella pneumoniae strain KSB2_1B plasmid unnamed6, complete
            sequence.
ACCESSION   CP024512
VERSION     CP024512.1
DBLINK      BioProject: PRJNA351909
            BioSample: SAMEA3357405
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: KSB2_1B
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/30/2017 11:04:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,094
            CDS (total)                       :: 5,976
            Genes (coding)                    :: 5,724
            CDS (coding)                      :: 5,724
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 252
            Pseudo Genes (ambiguous residues) :: 0 of 252
            Pseudo Genes (frameshifted)       :: 99 of 252
            Pseudo Genes (incomplete)         :: 154 of 252
            Pseudo Genes (internal stop)      :: 63 of 252
            Pseudo Genes (multiple problems)  :: 55 of 252
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB2_1B"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
     gene            62..220
                     /locus_tag="CTI57_30375"
     CDS             62..220
                     /locus_tag="CTI57_30375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="ATR13415.1"
                     /translation="MHEPPVQSDYHALFGNYQLESNPKRHDKSPLAAAISNWRYTREIF
                     RGLEVWA"
                     /name="replication initiation protein"
     gene            complement(396..1961)
                     /locus_tag="CTI57_30380"
     CDS             complement(396..1961)
                     /locus_tag="CTI57_30380"
                     /inference="COORDINATES: protein motif:HMM:PF00884.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATR13416.1"
                     /translation="MDIILKFKIIDCIKRGFGFYKWDVILISWISLVHFSLGYGVKVIY
                     VLSVFFGLKLLFCFSRFFYSSIILILSLIGFIYSPVGIIYGYPDINVVGSFLYTDSSES
                     KEFLIGTPFYIYLTSFLIVLIGFFLLKMPFSKKISSSRVKVFFFVFFVISSTWSAIRQG
                     GILNGDIALPEIRFVKDLYSAYKKVEGDRVSYSLIIKSKSIWSPNKSRGEYKNYILVIG
                     ESARRDYVHSFGGKYSNTPWLDSAPAMIFDNYISAGPSTMISLTNTLSLRKGSVLELNN
                     NVVTLASKAGFETWWLSNQGSKGHFDSPISLIGHSADYSEFIKSGSSDDRLISPDENLL
                     PLLDVALNKNKNENKLIVLHLMGSHPKACIRTNNTYDIDVGAKEVSCYIKSIEMTDQFL
                     SKVISLANQTGESWSVMYFSDHGLSYVNKDTQGAYLTHGDKTKENYEVPFFIINSNSEI
                     KEVVHQPRSALDFMTTFSQWLKISDRNIPSACNMLSNENCSNEDYVIDFNGNYIKFNDL
                     PSVP"
                     /name="hypothetical protein"
     gene            complement(3086..3304)
                     /locus_tag="CTI57_30385"
     CDS             complement(3086..3304)
                     /locus_tag="CTI57_30385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013023910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATR13417.1"
                     /translation="METPGIFIVLSGFAATDLSVRFRDACQGGGAYGKTAAPHPFFCPA
                     CHAVCLCSYPCTGNARRSRTAGAAATE"
                     /name="hypothetical protein"
     Typing          complement(2874..3089)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="88.128"
                     /coverage="100.0"
                     /evalue="3.41e-66"
     Typing          5..391
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.382"
                     /coverage="98.0"
                     /evalue="4.03e-89"
     Typing          3039..3377
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.941"
                     /coverage="98.0"
                     /evalue="6.18e-137"
ORIGIN
        1 gtttctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg
       61 tatgcacgaa ccccccgttc agtccgacta ccacgctctt ttcggtaact atcaacttga
      121 gtccaacccg aaaaggcacg acaaatcgcc attggcagca gccattagta actggaggta
      181 tacgagagag atattcagag gtcttgaagt atgggcctga ctacggctac actgaaagaa
      241 cagttttggt atctgcggtc tcgtataccc agctacctcg gttcagaaga gttacagctc
      301 aactgatccc tcgaaaagcc accttaccgg gtggcttttt cgtttacagg tcaggagatt
      361 acggccagaa cgaaaggatc tcaagaagat cctgtctatg gaaccgaagg caagtcatta
      421 aatttaatgt aattcccatt aaaatcaata acataatctt cattactgca gttttcatta
      481 gaaagcatat tacaagcact tggtatgtta cgatcgctaa ttttcaacca ttgagaaaat
      541 gtcgtcataa aatcaagtgc actacgtggc tgatgtacaa cttcttttat ttctgagtta
      601 ctattaataa taaagaatgg cacttcataa ttctctttag tcttgtcacc atgtgtcaag
      661 taagcacctt gtgtatcttt attaacgtat gacaatccat gatcagaaaa atacataaca
      721 gaccaacttt caccagtctg atttgcaaga gaaataactt tagataaaaa ttgatctgtc
      781 atctctatgg actttatata acatgatact tccttcgcgc ccacatcaat atcataggta
      841 ttattagttc taatgcaggc ttttggatgt gatcccatca aatgaagaac aatcaattta
      901 ttttcatttt tatttttatt taaagctaca tcaagtaagg gtaacaaatt ttcatctggc
      961 gagataagcc tatcatctga gcttccagat ttaataaact cactataatc tgcactatgc
     1021 ccaatcaatg aaattggtga atcgaagtgc cccttactgc cttgattgga taaccaccac
     1081 gtttcaaaac cagcttttga tgctagagtg acaacattat tattcagttc taaaacacta
     1141 cccttacgta aggataaggt attagttaat gagatcattg ttgaaggacc ggcagatatg
     1201 taattatcaa aaatcattgc tggggcacta tcaagccaag gagtgttact gtacttaccg
     1261 ccgaatgaat ggacataatc acggcgagca ctttcaccta taaccaaaat gtaattttta
     1321 tactcccctc ttgatttatt cggactccaa atactctttg atttaattat taatgaatat
     1381 gaaaccctat caccctccac cttcttatag gcggaataaa gatcttttac aaacctaatt
     1441 tccggcagtg caatatcacc atttaaaatt ccaccttgcc tgatagctga ccatgttgag
     1501 gaaatgacaa agaaaacaaa aaaaaacact ttaactctac tagaggatat tttttttgaa
     1561 aatggcattt tcagtaagaa aaaaccaatt aacactatta aaaaagaagt taaatatata
     1621 taaaacggcg taccaatcaa aaactctttg ctttcactgc tatcagtgta caaaaaagaa
     1681 cctacaacat taatatcagg gtaaccgtag ataataccta caggggaata tataaaccct
     1741 atcaatgata atattaaaat tatcgaactg taaaagaatc tactaaaaca aaataacagt
     1801 ttcaatccaa aaaaaacaga taatacatat attaccttaa ctccataccc aagagaaaag
     1861 tgaacaagtg aaatccaaga aattaaaata acatcccact tgtaaaaacc aaacccacgt
     1921 tttatgcaat caatgatttt aaatttcaaa ataatatcca atttttattg tttaacataa
     1981 tcccacttac gcgcaccgcg aaaacggcct cagggcgttt ctgacgggaa accggcaaaa
     2041 caggcaacaa atcccccgaa aacccgccag aaacgcgcag gctctgtttt tacggcatgc
     2101 atgagtatgc gcgcaagtgc atagtgcgga aattcacgcc attcagccgg ggtaagtcct
     2161 gtttccgacg ttcttcccac ccgaaaacgg tgaacgggga cgcaatttat tgcgtggccg
     2221 tgaggcgttg ccggcgcagc cggaaaaacc gttttcagca cgttcaggaa ggggggtcgg
     2281 atgtacgaat agttaagagg tcagatctga cgtaagaatg atgtaaaagt gatgtcattc
     2341 tgtagttgat ttgtagttta tctgtagtta gctctctgaa aggcatgatt aaacatcaga
     2401 ttttgtagtc tttttgtagt ttacctgtag tcaggaaaac tactccgttc atgcgagcat
     2461 gaacggagta gtgaaggacg cttttttgtc atatcagcct cttaacctgc gcagatagcc
     2521 cagtcaaaat gatgtttttt taacccttaa atggtcatac acctaacgca gccgcgattt
     2581 tcatcgcaac tgcatgaatt acataaaaaa aatgggatac tccatttatt acggtaatac
     2641 gcaaggtatt acaatcaact gtaagcacat gaaaataaag aaataagatg taataccacc
     2701 gtattacacg tacgctggcc agcgaaaaag ctataacgct tcccggccga caggtggccc
     2761 ggaatccacc ggggcgtgca ggttatcttc agtaaccttc gccattaaat caaaaaggtc
     2821 atactcgccg ggaagctgcg tatcagcagc atcccgttgt tcatcatgtt cagatggcat
     2881 ggggtgatat ccagtagcgg agaggttgtt agcgggtttc ggggcgcagc cctgaaccag
     2941 tcacgcagcg ctagcggagt gtatactggc ttaatatgtt acggcggagt aacacttaat
     3001 gaaagtgctt catgtaacag gggaaaatgc agctacaggg cgtacgggag aaaatggggg
     3061 ttacggtgat attacgcctc ctcgctcact cggtcgctgc cgctcctgcc gttcggctgc
     3121 ggcgggcgtt accagtacac ggataagagc aaaggcatac ggcatgacaa gctgggcaaa
     3181 aaaaaggatg cggcgctgcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa
     3241 atctgacgct caaatcagtg gcggcgaaac ccgacaggac tataaagatc ccaggcgttt
     3301 ccacctggta gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt
     3361 ccgctgttat ggccgcg
//
