LOCUS       CP024511                3514 bp    DNA     circular BCT 06-NOV-2017
DEFINITION  Klebsiella pneumoniae strain KSB2_1B plasmid unnamed5, complete
            sequence.
ACCESSION   CP024511
VERSION     CP024511.1
DBLINK      BioProject: PRJNA351909
            BioSample: SAMEA3357405
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: KSB2_1B
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/30/2017 11:04:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,094
            CDS (total)                       :: 5,976
            Genes (coding)                    :: 5,724
            CDS (coding)                      :: 5,724
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 252
            Pseudo Genes (ambiguous residues) :: 0 of 252
            Pseudo Genes (frameshifted)       :: 99 of 252
            Pseudo Genes (incomplete)         :: 154 of 252
            Pseudo Genes (internal stop)      :: 63 of 252
            Pseudo Genes (multiple problems)  :: 55 of 252
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB2_1B"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
     gene            complement(15..356)
                     /locus_tag="CTI57_30340"
     CDS             complement(15..356)
                     /locus_tag="CTI57_30340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="ATR13414.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            554..912
                     /locus_tag="CTI57_30345"
                     /pseudo=""
     CDS             554..912
                     /locus_tag="CTI57_30345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532398.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            976..1107
                     /locus_tag="CTI57_30350"
     CDS             976..1107
                     /locus_tag="CTI57_30350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="ATR13413.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGNRISRAS"
                     /name="replication initiation protein"
     gene            1527..1718
                     /locus_tag="CTI57_30355"
     CDS             1527..1718
                     /locus_tag="CTI57_30355"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATR13410.1"
                     /translation="MLKKNEIILIKSCGNSKTKIFLRGVDEGLMIDASFEDIKFAINNN
                     LHDLLGIQAETTKLKLAK"
                     /name="hypothetical protein"
     gene            complement(2132..2347)
                     /locus_tag="CTI57_30360"
     CDS             complement(2132..2347)
                     /locus_tag="CTI57_30360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="ATR13411.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="mobilization protein"
     gene            complement(2354..2851)
                     /locus_tag="CTI57_30365"
     CDS             complement(2354..2851)
                     /locus_tag="CTI57_30365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="ATR13412.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLQMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="mobilization protein"
     gene            complement(2832..3491)
                     /locus_tag="CTI57_30370"
                     /pseudo=""
     CDS             complement(2832..3491)
                     /locus_tag="CTI57_30370"
                     /inference="COORDINATES: protein motif:HMM:PF03432.12"
                     /note="incomplete; partial on complete genome; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /name="nuclease"
     Typing          complement(442..614)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.655"
                     /coverage="100.0"
                     /evalue="9.54e-56"
     Typing          539..1085
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.909"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(22..335)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="9.860000000000001e-59"
     Typing          complement(2017..3512)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.198"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 cccggggatg gaatttaaca atcatcgtcc ggcccttttt ccagtacggc atgccgcaac
       61 cgttccagac cggcatcgat cgccatcagc gctgccacga tctgcacccg gtcatgaccg
      121 gcaccaccgg cgttcacttt ccgggcgatc tggttcaggt tattgcccat gccggcaagc
      181 tgacgcagca gcgcgggcga aatcgaggga agcctgccgg cgcgtgtcgg tttctcatca
      241 aggcaggtct ggcgcatcca cgccgccagt tgttttccgt cgcaccgctc aagcaaccgc
      301 cggtgttcgt cttccgtcac ccacatcgtg agcatcttgt tgcgtttatc tgccagaatc
      361 gcctcctgat aacgccgtga cggacgggaa agcctttccc ggtcggcgcg gggttggaca
      421 agccgcaggc gcgtcagtgg ggtttcgggg cgcagccctg aaccagtcac gtagcgctag
      481 cggagtgtat actggcttag tatgttggca tgagtgcgat aatcaggaaa gcgccttatg
      541 cggcagaaga aaaatgctgc agcagagcgt aagcagaata tgcgatacag aagatattcc
      601 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtgccggct
      661 tacgggcggg gcggaaattt ccaggaagat accaggaaga aacttaacag ggaagcgatg
      721 aggccgcggc gaagccgttt ttccataggc tccgcccccc tgacaagcat cacaaaatct
      781 gacgctcaaa tcagtggcgg cgaaacccga caggactata aagatcccag gcgtttcccc
      841 ctggtagctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
      901 tgttatggcc gcgtttatct cattccacgc ctgacactca gttccgggaa ggcagttcgc
      961 tccaagctgg actgtatgca cgaacccccc gttcagtccg actgctgcgc cttatccggt
     1021 aactatcgtc ttgagtccaa cccggaaaga cacgccaaag cgccactggc agcagccact
     1081 ggtaacagga ttagcagagc gagttaatgt aggtgatgct acagagttct tgaagtggtg
     1141 gcctaactac ggctacactg gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     1201 tacctggtta agcagttccc caactgactt aaccttcgaa aaaccgccct gcaaggcggt
     1261 tttttcgttt acagagcaag agattacgac gaggctgaat gaatctcaag atttcattta
     1321 gcctgccacc cgatcattca aagttttggc gtagttctga cccgctcccc gtgatttgtt
     1381 acatacccac cacagggcat tagttttttt atttttttga tgatatgtac aaaatcagtg
     1441 gttaggaata atcttagcag ccttaacaaa cacacacaaa aacaacgata atgattcatc
     1501 tgacgacaac aaacaatgac aatgtcatgt taaaaaaaaa tgaaattatc ttaataaaat
     1561 catgtggtaa ctcaaaaaca aaaatttttt tacgaggtgt ggatgaggga ctcatgattg
     1621 atgcaagttt tgaagatata aaatttgcta tcaacaataa cctccacgat cttctcggaa
     1681 tccaagccga aaccacaaaa ctaaaactag caaagtgata cagcccctga agcatgaagg
     1741 atcgaaataa agccaccgat ttaccataac gtcttcttac ccgcaccgcg ccggaggcac
     1801 tcagggcgtt tctgatggct gacgggcaaa aggtggtggc aagtacaccg aaaaccacca
     1861 gaaacgcacc tgatttattt tacgcatatg catcacttat tcacatcact gcatagccgg
     1921 tgatttcggt taaaaaagac tctctgtgtg ctgaaatcgc agaaaatggc catgatcccc
     1981 gtcacgctca ggccacctga gacgggcgtt tatcagtctc catactccct gtcaccatga
     2041 gataatgtgc gttgccgttc cggtaccaga tctgctgtct tctctggttc cttttccgtt
     2101 ctttgcccgt atcgctgttg atcaatctca ttcagcctgt cgataaccgg cgcagctgct
     2161 cgctcaaact ctgcacctgc ctgctcaagc ccgacacgcg ctcgctcagc gccgcgttct
     2221 cccgttgcat aagaccagac atcttccgcc attcctcgaa ggcgctctcc cactcgtcca
     2281 gccttctcga gtagtcctgc tgtagctgct ctaacgcgct cagcaattgt ttttccagtt
     2341 ccgtcattct gctctactcc cgtaccagct tcaccgcgtt ccgcgtcttg ttgtccaccg
     2401 tccggcccgt ttcggctttc atcccctccg gcagcaccag aaaccgtccg ttgcggttct
     2461 ccagataggt cacgccccac gtctgcatct gcagtttcga cagcgtatct ctctgctgcc
     2521 tgatttccgc caggttcgcc gctatccggt tcccctggta ccacagcacc ccgctcaggc
     2581 tggcgaacag cagccctgac accatggtga tggtcagcca cgtccgcccg accattcgca
     2641 gcacgctcag ccggttcgac tgcatcgccg cgttcatccc ctgttcgtgg gcgctgatgg
     2701 cgtcgttgat tctcttcgcg cttgcgttca gttccgattt gacaacgttc tcgtgcttgc
     2761 tgaatgcggc tttcagcctc tcttcggtaa gctgctgctg tgctttcgat ttctcctcga
     2821 agtccctcga ggccgttaaa agactgctca taaatggctc ccctcagtcg gatatttcgc
     2881 cccccgtccg ggtcggcaat gctgatactg ttttttgtgg ttctgaccac ctcaaaaccg
     2941 gccgctgtga gcgcccctgt gacgtcatca cgcgttttca gctctcctga tgaggctaac
     3001 atcagcaaac cccgtgtaat cgtctctgcg gcttcctgct tcatttctgg cagtcctgaa
     3061 ggcgtgacca gcgcccgccg gttctccggg tcgttcggat catgtagccc cagcctgccg
     3121 ttgaccactg tctgccacgc atcgatgcgc ggccggtcgg cacgatcgta atacggctgg
     3181 aggcgtttcc ccgtcagtaa ttcggtattg gggatcagaa aattcagctc aagacgaccc
     3241 ttgtctgcgt gttccaccca cagaatgctg tactggtctt tatcgagtcc gggcatcaga
     3301 acccgctcaa agctggccat cagtttttca cgctgacccg gcggcaggtc cgcctccgcg
     3361 aatgacagca ccccggaggt atattttttc acatacggcg acgcatcaat cagctcccgg
     3421 acttcttccg gcttcccctg caggacgctg gcaccttcac gctgccggtc tttacccaga
     3481 agataatcca caggaccggc gcccccgccg cgac
//
