LOCUS       CP024506                2936 bp    DNA     circular BCT 06-NOV-2017
DEFINITION  Klebsiella pneumoniae strain KSB2_1B plasmid unnamed10, complete
            sequence.
ACCESSION   CP024506
VERSION     CP024506.1
DBLINK      BioProject: PRJNA351909
            BioSample: SAMEA3357405
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: KSB2_1B
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/30/2017 11:04:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,094
            CDS (total)                       :: 5,976
            Genes (coding)                    :: 5,724
            CDS (coding)                      :: 5,724
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 252
            Pseudo Genes (ambiguous residues) :: 0 of 252
            Pseudo Genes (frameshifted)       :: 99 of 252
            Pseudo Genes (incomplete)         :: 154 of 252
            Pseudo Genes (internal stop)      :: 63 of 252
            Pseudo Genes (multiple problems)  :: 55 of 252
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB2_1B"
                     /db_xref="taxon:573"
                     /plasmid="unnamed10"
     gene            complement(1613..1756)
                     /locus_tag="CTI57_25860"
     CDS             complement(1613..1756)
                     /locus_tag="CTI57_25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="ATR12651.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDLSPLAAATGNGFAEQRNV
                     EV"
                     /name="replication initiation protein"
     gene            complement(1781..2224)
                     /locus_tag="CTI57_25865"
     CDS             complement(1781..2224)
                     /locus_tag="CTI57_25865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014343523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /protein_id="ATR12654.1"
                     /translation="MNLAMFSAVFCCFVIKGLRASVLSVKPVSFPLPRSLSRYAPVVRL
                     RRAAGACIEKLEKTERSKEEKEEKKERPRRCRFSIGSAPLTSIAETDAQISGGETRQDY
                     KDTRRFPLAAPSCALLFLPFGLPVSLRCYGRVYLIPRLTLSSG"
                     /name="plasmid replication initiation protein"
     gene            2181..2372
                     /locus_tag="CTI57_25870"
     CDS             2181..2372
                     /locus_tag="CTI57_25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="ATR12652.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDSLQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2372..2794
                     /locus_tag="CTI57_25875"
     CDS             2372..2794
                     /locus_tag="CTI57_25875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="ATR12653.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     VSELIRVFGSLDGVTDTVKYDVKSDAISTEVNTIGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHDPSSPAHSPWWKFWKKS"
                     /name="DNA-binding protein"
     Typing          complement(1649..2014)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.095"
                     /coverage="85.0"
                     /evalue="1.36e-162"
ORIGIN
        1 acaacaatgg tttaatgaaa aataaatgtg cggtgatgtg ttacaaagtg cagatccaag
       61 tcgtggacga ctctatggcg aatcgctgac gaattgccca cgattcatat gtgaatcacg
      121 ctttattatc atattgttat gatgaatcga ctcttgattc aattcatcac tttgggtgtt
      181 ttttgttcat ggtgactctt gattcaattc acgattcgaa tcgccggtga atcgtgtgct
      241 aagtcgtccg gtgacttgcc ataacgtgat tgccggccgt cgacgaaccc gattcctgaa
      301 cgtgctgaaa accggttttc cggctgcgcc ggcaacgctt cacggccacg caataaattg
      361 cgtccccgtt caccgttttc ggatgggaag aacgccggaa acaggactta ccccggctga
      421 atggtgtgaa tttccgcgct atgcacttgc gcgcatactc atgcatgccg taaaaacaga
      481 gcctgcgcgt ttctggcggg ttttcgggtg gtttgttgtc tgttttaccg gtttcccgtc
      541 agaaacgccc tgagagcctc tcaggcggtg ctgataacgg atgttatggt aaatgttgga
      601 tagcaaggac tatcagtccg gcaatgctac atatcattgc taatggattt acgagcaaaa
      661 tgccgaatgt tgtcgcagat aatccgcgaa caatgatatt ggcaagaagt ccgccaacaa
      721 tgagggataa aaaggtataa ccagcaactt tcagtaacga cagatctccc catatcacct
      781 ctactatcgg atagataaaa acaccaacgc cagcaggaag cgtagtaacc atcaacagta
      841 aatggctttt agtgggaccg tactctctga catttccaac tttcatcatg tgtgtgatga
      901 atgacgatat ccaccatagg atcatgatca taaggaaatt cataattatt tactctcgat
      961 gcgtagatca ggtgctttct gtagatttgg tcgtacatac tcgctgtctc tttcgtcaag
     1021 acaacggaag tagagtgtta ctgtggtatc tatccccatt cttgtgttag ccacgttttc
     1081 gttagtgagc aggaattttt taccgaggct attacatttc tcagaacctg ttttaaaggc
     1141 actttgacgt gctgatgccg catctctaaa accatcagca gttgctgaaa ccgtatatgt
     1201 atccctacct aactcaagaa cgttgctggt agaaacacaa ccaactgtaa gcagcgatat
     1261 tgataaaata cctaacctat aaatcattga ttttattacc caagttgggt gattgtggat
     1321 catgattata tctgtttttt ttattattaa aatcaagggg ttcaataatt ttcttcaaag
     1381 aactggtgtc gtggagtggt gctggtttcc ctttgggggg gagagggatg gaataggatc
     1441 ctcttgagat cctttctgtt gcgtcgtaat ctcttgccct ggaaacgaaa aaaccgccca
     1501 gcaaggcggt tttttcgaag gttcttagag cagcaactct ttgaaccgag gtaactggct
     1561 ttcgcagagc acagaaacca aattagttat tacagtgtag ctgtacttca cctcacactt
     1621 caacatttct ttgttctgcg aatccgttac cagtggctgc tgccagtggc gataagtcgt
     1681 gtctttccgg attggactca agacgatagt taccggataa ggcgcagcag tcggactgaa
     1741 cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca
     1801 ggcgtggaat gagataaacg cggccataac agcggagtga caccggtaaa ccgaaaggca
     1861 ggaacaggag agcgcacgag ggagccgcca gggggaaacg cctggtatct ttatagtcct
     1921 gtcgggtttc gccaccactg atttgagcgt ccgtttctgc gatgcttgtc aggggggcgg
     1981 agcctatgga aaaacggcaa cgccgtggcc tttccttttt ttcctccttt tcctcctttg
     2041 accgctcggt tttctcgagc ttttctatgc atgctcctgc cgctcgccgc agtcgaacga
     2101 ccggagcgta gcgactgagt gagcgaggaa gcggaaaaga gacaggtttt acactgagca
     2161 ctgacgctct gaggcccttt atgacgaaac aacagaaaac ggcgctgaac atggctaaat
     2221 tcatccagaa tcagtctctg ctcctgctgg agaagcttaa cgagcttgat cttgatgctg
     2281 aggccgacct ttgtgagaaa ctccacgatg atgcagagca tcttttccgt acactctcct
     2341 cgagactgga ttcattacag gatggtaact gatggccaga gtgtctatta gtgaagctgc
     2401 acggcttgtt aaggttagtc gtcccactat ttacaagatg attaacagcg ggaagttaag
     2461 ttatacctct gttgtaaagc acgggaagga tattaaagtt attgatgtat ccgagcttat
     2521 tagggtgttt ggttctcttg atggtgtaac tgatactgta aaatatgatg taaaaagtga
     2581 cgcaatttct acagaggtta acaccattgg tttacatgat ttacaacata gaatcgcttt
     2641 acttgaagct gaaaacgatg gtcttaaagg ggctgttaaa gcacgcgatg agcatattga
     2701 ttcccttcgc caggctatgc agcttttgga gcacaagcac gacccctctt caccggctca
     2761 ttctccgtgg tggaaattct ggaaaaaatc ctgatcctgc catgtttcag ttttgctctc
     2821 cggcgatccc ggggagttcc tgaaccttcc caaaaatcgc cagatctctg aacgctcctc
     2881 accactaaaa atacccacac cccacgcaaa attaaaattt tgcagattcc ttattt
//
