LOCUS       CP024503                3223 bp    DNA     circular BCT 06-NOV-2017
DEFINITION  Klebsiella pneumoniae strain KSB1_4E plasmid unnamed4, complete
            sequence.
ACCESSION   CP024503
VERSION     CP024503.1
DBLINK      BioProject: PRJNA351909
            BioSample: SAMEA3357325
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 11-JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: KSB1_4E
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 124x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/30/2017 11:05:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,693
            CDS (total)                       :: 5,575
            Genes (coding)                    :: 5,400
            CDS (coding)                      :: 5,400
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 78 of 175
            Pseudo Genes (incomplete)         :: 94 of 175
            Pseudo Genes (internal stop)      :: 40 of 175
            Pseudo Genes (multiple problems)  :: 33 of 175
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB1_4E"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
     gene            complement(172..412)
                     /locus_tag="CTI56_28440"
                     /pseudo=""
     CDS             complement(172..412)
                     /locus_tag="CTI56_28440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            378..590
                     /locus_tag="CTI56_28445"
                     /pseudo=""
     CDS             378..590
                     /locus_tag="CTI56_28445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001692121.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing start; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            615..785
                     /locus_tag="CTI56_28450"
     CDS             615..785
                     /locus_tag="CTI56_28450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="ATR07700.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="replication initiation protein"
     gene            complement(994..1326)
                     /locus_tag="CTI56_28455"
     CDS             complement(994..1326)
                     /locus_tag="CTI56_28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005969762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug SMR transporter"
                     /protein_id="ATR07701.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="multidrug SMR transporter"
     gene            complement(1393..2886)
                     /locus_tag="CTI56_28460"
     CDS             complement(1393..2886)
                     /locus_tag="CTI56_28460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="ATR07703.1"
                     /translation="MIVKFHPRGRGGGGGPVDYLLGKDRQRDGASVLQGKPDEVRELID
                     ASPYAKKYTSGVLSFAEQDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHRDKGRLE
                     LNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRVLVSPSAL
                     PEAKQEAAQAITSGLLALASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGRN
                     IRLKGAIYEQSFNAGEGLRAEIESAAADYRRDAESRIQRAREVCQSGTERKREENQRRH
                     PRPRPEAVLSHEPAHERDAAHGQPDVADHRSGLRAADSVERGHSVVAGAADTRELYDHP
                     GAEEHAGHAVREEQRRTALDLRRAETPLREGEPGSGTVRRGLELDDTGGEIAHDGAGKT
                     VAERIRAATAGLLEKAGRVGERLRGMADDVWSYSTGERGAERARHGLEQAGAEFERAAA
                     PVVERLNAIETHRQQERAVQHQKALELERSQWQHHGPSL"
                     /name="nuclease"
     gene            complement(2876..3220)
                     /locus_tag="CTI56_28465"
     CDS             complement(2876..3220)
                     /locus_tag="CTI56_28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="ATR07702.1"
                     /translation="MADKRSKMLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPAR
                     AGKLPSISPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="plasmid mobilization relaxosome protein MobC"
     ARG             complement(995..1326)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          complement(73..264)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          244..727
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.756"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          complement(1397..2886)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggcctcctga taacgccggg acggacggga aatcttttcc cggtcggcac ggtgttggac
       61 aagccgcagg cgcggcagtg gttttagcgg gtttcggggc gcagccctga accagtcacg
      121 tagcgctagc ggagtgtata ctggctaaac atgttatgac ggagtggcaa tttatgaaag
      181 tgcttcatgt gacaggggaa aaatgcggct gccgggcgta agtgcaaaat gcaggtggtg
      241 atgaaatgac gcctcctcgc tcactcggtc gctacgctcc tgccgtgaga ctgcggcggg
      301 cgttaccagc tcacagatta cgggataagc aaatgccgct cgaagcagga aaggccaaga
      361 aaaggatgcg gagttgccgt ttttccatag gctccgcccc cctgacgagt atcacgaaaa
      421 tgacgctcaa atcagcggtg gcgaaacccg atgcggctta aagataccag gcgtttcccc
      481 ctggtggctc cctcgtgctc ccccgcattc ctgcctttcg gtttaccggt gtcattccgc
      541 tgttatggcc gcggtttctc attccacgcc tgaaactcgg ttccgggtag gcagttcgct
      601 ccaggatgga ctgtatgcac gaaccccccg ttcagtccga ctaccacgcc cgttccggta
      661 actatcaact tgagtccaac ccggaaagac acgacaaatc gccagtggcg gtagccattg
      721 gtaactgggt tcacggagca gagattgtag agaatgccta cagagttctt gaagtgaaag
      781 cctgagtgcg gctacactga aaggacagtt taggtatctg cgctccgtga aagccagtta
      841 ccatcggaac aaagagttgg tagctctgaa ttccggcaaa cgaaccaccg ttggtagcgg
      901 tggttttttt gtttacaggg aaaaagatta cgcgcagaaa gaaaggatct caaggaaatc
      961 cttttgcttt ggccaatatg cttcggtaaa aagttaatgc gcagaagatt ttgaaaaaat
     1021 attaataacc aaaactccaa cgcaaataag gaacattcct gcaattgcgg gccaatcaag
     1081 tttttgaccg ctaaccaacc aaccagctag gcttattagt acaattcccg caccagacca
     1141 aattgcgtaa gcaataccgg taggtatatt ttccaatgtt tgggaaagca gataaaaggc
     1201 agcgcaataa cttattgcag ttcctacaga cggccacagg cgtgtaaatc cttctgaata
     1261 tttcataaag gtggtgccta tgacttcagc cacaatagcc cctcccagga aaatatatgt
     1321 attcataaaa atacctcatg ttgaaaaatt taaatatctt tacataacaa ctaattaata
     1381 gaacccggct tatcatagcg aaggaccatg atgctgccac tgcgatcgtt ccagctccag
     1441 cgctttctgg tgctgcaccg cccgctcctg ctgccggtga gtttctatcg cgttcagcct
     1501 ttcgacaacc ggcgcagctg ctcgctcaaa ctctgcacct gcctgctcaa gaccgtgacg
     1561 cgctcgttca gcgccgcgtt ctcccgttga ataagaccag acatcgtccg ccattccgcg
     1621 aaggcgttct cccactcgtc cagccttttc gagtagtcct gctgtagctg ctctaatgcg
     1681 ctcagcaact gtttttccag ctccgtcatg tgctatttcc ccgccagtat catccagctc
     1741 gagtcctctc cgaactgtcc cgcttccggg ttcaccctca cgcagcggcg tctctgctcg
     1801 ccgcaggtcg agagctgtac gccgctgttc ctctctgaca gcatggcctg cgtgctcttc
     1861 tgctcccgga tggtcgtata gttctcgagt atctgctgcc cctgccacca cagaatgccc
     1921 gcgctcgacg ctatcagcag cgcggagacc agaacgatgg tcagccacgt ctggctgacc
     1981 atgcgcagca tccctttcgt gcgctggctc atggctgagg acagcttccg gtcgtggtcg
     2041 aggatggcgg cgctgattct cttctcgctt tcgctcagtt ccgctctgac agacttctcg
     2101 tgctcgctga atgcggcttt cagcatctcg ccggtagtct gctgctgcgc tttcgatttc
     2161 tgctctaagt ccttcgccag cgttaaaaga ctgttcatag atggctccct tcagtcggat
     2221 gtttcgcccc ccgtccgggt cggcaatgct gatgctgctt tttgtggtgc gcaccacctc
     2281 aaaacctgcg ctttccagcg cctcagtgac gtcctgacgc gttttcagct ccccggatga
     2341 ggcaagggca agcaggccgc tcgtaatcgc ctgggcggct tcctgcttcg cttccggcag
     2401 cgcggaaggc gagaccagca cccgccggtt ctccggcgcg ttcgggtcat gcagccccag
     2461 cctgccgttc actatggtct gccaggcatc gatgcgcgga cggtcggcgc ggtcgtaata
     2521 cggctgtagc cgcttgccgg tcagaagctc cgtgttcggg atcaggaagt tcagctccag
     2581 ccgccccttg tcccggtgct caacccacag cacgctgtac tggtctttat cgagtccggg
     2641 catcagaacc cgctcgaaac tcgccatcag cttttcgcgc tggccgggcg gtaaatcctg
     2701 ttcggcaaaa gacagaacgc cggaagtgta ctttttggcg tagggcgagg cgtcgataag
     2761 ctcccggact tcatccggct tcccctgcag aacgctggcg ccgtcgcgct ggcgatcttt
     2821 acccagcaga taatcgaccg gcccgccgcc gccgccgcgc ccccgaggat gaaacttaac
     2881 gatcatcatc agcacccttt tccagtacgg catgccgcaa ccgctcgagt ccggcatcga
     2941 tggccatcag cgcggcgaca atctgcacgc ggtcgtgtcc gcttccacca ccggcgttaa
     3001 cctggcgggc aatctggtta aggttgttcc ccatgccggc aagctgacga agcagcgccg
     3061 gcgagatcga cggaagtttg ccggcgcgtg cgggcttctc gtccaggcac gtctgccgca
     3121 tccaggccgc gagttgctta ccctcgcagc gctccagcag acgccggtgc tcgtcctcag
     3181 tcacccacat ggtgagcatt ttgctgcgtt tgtctgccaa tcc
//
