LOCUS       CP024502                4064 bp    DNA     circular BCT 06-NOV-2017
DEFINITION  Klebsiella pneumoniae strain KSB1_4E plasmid unnamed3, complete
            sequence.
ACCESSION   CP024502
VERSION     CP024502.1
DBLINK      BioProject: PRJNA351909
            BioSample: SAMEA3357325
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 11-JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: KSB1_4E
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 124x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/30/2017 11:05:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,693
            CDS (total)                       :: 5,575
            Genes (coding)                    :: 5,400
            CDS (coding)                      :: 5,400
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 78 of 175
            Pseudo Genes (incomplete)         :: 94 of 175
            Pseudo Genes (internal stop)      :: 40 of 175
            Pseudo Genes (multiple problems)  :: 33 of 175
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB1_4E"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
     gene            complement(5..1264)
                     /locus_tag="CTI56_28415"
     CDS             complement(5..1264)
                     /locus_tag="CTI56_28415"
                     /inference="COORDINATES: protein motif:HMM:PF03389.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobA"
                     /protein_id="ATR07695.1"
                     /translation="MALYRLEMQNVSRSDGVSSVAKAAYRHRSVMIDERTGEIHGEKSA
                     NRDDLVYAEILAPEGTPDFLIKSSNDLWNYVEKTEKRKDARTAKEFKITLPCELTNEQN
                     IKLLKEYLTKNFVSKGIICDFVIHNDKDNKNPHAHVMITTREITLSGFGKKVRSWDEEK
                     TLHDWRKQWAKVQNQHLKNAGLKSRVSHRTLEEQKNIMIDLAKKAEDAKDFDKSILYLA
                     KAIELDRPPMQNLSRKKWRTKEGQEQRKRDQAIRDRARADARAFRLLRGVAVVDVASFV
                     VHPLEKKEQSKNNDFSEKKSWWRSTVNFIKNIKAKFSTKKDIKNDVHASDDLMTDPVTG
                     LPITKAKWQEMSDRANVRTPQPAISYQVKQSDGEGLKTAQNALQDVLDVSEGKSLKVKS
                     VPVPGSRKRRVVEEDSSDNG"
                     /name="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobA"
     gene            1560..1955
                     /locus_tag="CTI56_28420"
     CDS             1560..1955
                     /locus_tag="CTI56_28420"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATR07696.1"
                     /translation="MNKKEIEKRLLIFTSEIDIEDIKNDFINDEVNEDVFNRCLKLYYA
                     IDDAIYNNKKSLFEALIYLINKDRENKDHDEPIITDYDLVYLTLNNYINIYELKKLFLL
                     QKNINDFDFKSMISEIEKIKREVEYGK"
                     /name="hypothetical protein"
     gene            1945..2223
                     /locus_tag="CTI56_28425"
     CDS             1945..2223
                     /locus_tag="CTI56_28425"
                     /inference="COORDINATES: protein motif:HMM:PF06412.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATR07697.1"
                     /translation="MGSKKTLEQRMALAQDRLSKLKKEASQKERKAREHKKFTVGGEVT
                     RVFGLDYDLALVVGFLEHFNKLSDEQKQALKNNGERLLKSRLENADK"
                     /name="hypothetical protein"
     gene            complement(2637..2906)
                     /locus_tag="CTI56_28430"
     CDS             complement(2637..2906)
                     /locus_tag="CTI56_28430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="ATR07698.1"
                     /translation="MKGNEIRKGLTQKEMAKKFNLSLSTVKKYTSQPREHYESEAENRR
                     KTAYTLHHKKGLKWREVAEKMNTTENAVKALAKRYKQIDQENRG"
                     /name="plasmid replication protein"
     gene            complement(2903..3760)
                     /locus_tag="CTI56_28435"
     CDS             complement(2903..3760)
                     /locus_tag="CTI56_28435"
                     /inference="COORDINATES: protein motif:HMM:PF08708.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="ATR07699.1"
                     /translation="MILQKEYSRSDFINQIISRPFCTDDFKRDGIYRTNKRRALNSVYI
                     EHNNDSFINSIVFDIDSDTAAIAWQDADIPKPNFITQNPANGHAHLFYALSSPVCITEN
                     ARRKPQKLLKGVIEGLTERLGADPCYTGKITKNPLNPRWRTFWNEQPRFELNYLCEFID
                     TNKRVKKEVRKSIVAEGRNTALFDSLRFYAYSIIFKYQKNDDFCGFISALQEEAENIND
                     SFEDQLSFKEINHTIRSISSWVWGTFDSDTFSEIQSNRAKKAKGKTKTNTKAKKLIEFI
                     GVKK"
                     /name="replication protein"
     Typing          complement(9..1174)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttagctaacc gttgtcactg ctgtcttcct cgacaactcg acgcttacga gaacccggaa
       61 ccggcacaga cttcaccttt aacgatttac cctctgatac gtctaaaacg tcctgtaacg
      121 cattctgagc cgtttttagc ccttccccgt ccgattgctt aacctgatat gagatcgcag
      181 gctgtggagt ccttacgttt gccctgtcac tcatctcttg ccatttcgct ttggtgattg
      241 gtaaacccgt caccggatca gtcattaaat cgtctgatgc atggacatcg tttttaatat
      301 cttttttagt tgagaactta gctttaatat tttttataaa attaacagta gaacgccacc
      361 aagatttttt ttctgaaaag tcattatttt ttgactgctc ttttttctct aacggatgaa
      421 ccacgaatga agcaacatca acaactgcaa cacctctcaa caacctgaaa gccctagcgt
      481 cggctctcgc cctgtctctg atagcttggt cacgtttgcg ttgttcttga ccttcttttg
      541 ttctccattt tttacgtgat aagttctgca ttggtgggcg atctagttca atagcttttg
      601 ctagatataa aatagacttg tcaaaatctt ttgcatcttc tgctttttta gctaaatcga
      661 tcattatatt tttttgttct tctagtgttc tatgactgac gcgagatttt aagcctgcat
      721 ttttcaaatg ctgattttga acttttgccc attgtttacg ccaatcatga agggtttttt
      781 cttcatccca tgaccttact tttttaccaa atcctgaaag cgttatttct cttgtcgtaa
      841 tcatcacatg agcgtgcggg tttttattgt ctttgtcgtt atgtataaca aagtcgcaaa
      901 tgatgccttt actaacaaag ttttttgtta agtattcttt caatagtttt atattttgtt
      961 cattggtaag ttcacaaggc aaagttattt taaattcttt tgctgttctt gcgtcctttc
     1021 tcttctctgt tttttcaaca tagttccata gatcattgct tgatttaatc aaaaaatctg
     1081 gcgttccttc tggtgccaat atttcagcat aaaccagatc gtcacgatta gctgatttct
     1141 cgccgtgtat ttctccggtt cgttcatcta tcatgacgga acgatgacga taagcagctt
     1201 ttgcaactga gctgacacca tccgaacgac taacgttctg catttctaat ctatataaag
     1261 ccatataatt atatcctcat gagaacccct attttttctt ttgggtttgg gttttagttt
     1321 ttcgtaagaa aaacaggttt tagccgtcgg cagaccccgg cattgtttac aatgcgtaac
     1381 cgggcattcc ttagctttta tttttcgcta acgctcaaac taaaagagga atgaaaaaca
     1441 aactagcttt tctttcactg atatatataa attaactctg attaaattaa agtcaaattt
     1501 gacaatattt aaaagttacg atattgaata aaaaacaagc attatggatt ggtggtaaaa
     1561 tgaataaaaa agaaatagaa aaaaggctat tgatttttac aagcgagatc gacattgaag
     1621 atattaaaaa cgatttcatc aatgacgaag ttaatgaaga tgtttttaat aggtgtttaa
     1681 agctgtatta cgctatcgat gatgcaatct ataacaataa aaaatcattg tttgaagcgt
     1741 tgatatattt gataaataaa gatcgtgaaa ataaagatca tgatgaacca attataactg
     1801 attatgatct tgtgtattta acactaaata attacataaa tatctatgaa ctgaaaaagt
     1861 tgtttttatt acaaaaaaat atcaatgatt ttgattttaa aagtatgatt tcagagatag
     1921 aaaaaattaa aagagaggtt gagtatggga agtaaaaaaa cgttagagca aagaatggca
     1981 cttgcacaag atagattatc taaacttaaa aaagaagcat cgcagaaaga aagaaaagct
     2041 agagaacata aaaaatttac tgttggcggt gaagttacaa gggtgtttgg tttagattac
     2101 gatctagctc ttgtagtggg ttttcttgaa cactttaaca agttaagcga tgagcaaaaa
     2161 caagcgttaa aaaacaatgg tgaaagattg ctaaaatcac gtctagaaaa cgctgacaag
     2221 tgacagattt ctaatctgac acttggaagg gttgaacaag caagaacccg ccgctggcgg
     2281 ggcttgtgcg ttagtcttca ccgtagcccg gctttgtgat atgtttaaaa aacatatcat
     2341 ccgggagagt aaaaaatgtc tcaaaaaaaa gagcaaaaaa cagcagaaaa accaactgca
     2401 gggccacttc gtaaacacag atctagagct gaaaaaatag acgatttaaa aaagcagatt
     2461 agcgaactaa gaaaagcaga aagagaagaa cgacaacgag caagagacga aaaattaatt
     2521 attcttgcaa aagctgttta tgaaaaggca ggaaaaccaa aagatattga agcattaatt
     2581 gatttcgtaa agtctgaaga tttcaagatt gaatataaat aaagctaagg ggcattttag
     2641 cccctgtttt cttggtcgat ttgtttgtat cttttggcta atgctttaac ggcattttct
     2701 gttgtattca ttttttcagc tacttcacgc cattttaagc cttttttatg gtgtaacgta
     2761 taagctgttt ttcttctgtt ttctgcttct gattcataat gttctcttgg ttgagaagtg
     2821 tattttttta cagtgcttaa agaaagattg aactttttag ccatctcttt ttgtgttaag
     2881 ccctttctta tctcgttgcc tttcattttt ttactcctat aaattctata agtttttttg
     2941 cttttgtatt ggttttagtt ttacctttcg ctttctttgc tctgttgctt tgtatttcgc
     3001 tgaaagtatc actatcaaaa gtcccccata cccaagatga aatgcttctt attgtgtggt
     3061 ttatttcttt aaagcttagt tgatcttcga atgaatcgtt tatgttttca gcttcttctt
     3121 gtaatgctga aatgaaaccg cagaaatcat catttttttg atatttaaaa attatgctgt
     3181 aagcgtaaaa acgcagacta tcaaataacg ctgtattcct tccctctgca actattgatt
     3241 ttcttacttc tttttttact cttttatttg tatctatgaa ctcacataga tagttcaatt
     3301 caaatcttgg ttgttcgttc caaaatgtac gccatctcgg attgagaggg ttttttgtta
     3361 tcttgcccgt ataacaagga tctgcaccta atctttcggt gagtccttct ataacgcctt
     3421 ttaataactt ttgtggcttt cttctggcgt tttctgttat acaaacagga gatgataagg
     3481 cataaaataa atgagcgtga ccattagccg gattttgagt tataaagtta ggcttcggta
     3541 tatctgcatc ttgccatgca atagccgctg tatcgctgtc aatatcaaac acaatagagt
     3601 taatgaagct atcgttgtta tgctcgatat aaacgctgtt taaagctcgt cttttgtttg
     3661 ttctataaat accatctctt ttgaagtcgt cagtgcaaaa tggtcttgat ataatttgat
     3721 ttataaaatc acttctgcta tattcttttt gtagtatcat tttttaatac aatcctttta
     3781 ttgtagatcc ctgattttct cctgtcgcca aacattcgga aatcagggat ttttgttttt
     3841 atattactgt tctaacataa cctttcatgg tgtcaagtag tcaccactaa aaaagtgtca
     3901 agtggtcatt ttagccatat caggtaataa tgggtctggg ggtatttttg cgaagcacag
     3961 gccgaaggct tcaaatcatc acaagttaag ctatagcctg ataggtacag gccgtaggct
     4021 atgagcgaag ccctgtaatc ttatgtagga tctggctgtg ctgg
//
