LOCUS       CP024487                3223 bp    DNA     circular BCT 06-NOV-2017
DEFINITION  Klebsiella pneumoniae strain INF322 plasmid unnamed5, complete
            sequence.
ACCESSION   CP024487
VERSION     CP024487.1
DBLINK      BioProject: PRJNA351909
            BioSample: SAMEA3357193
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: INF322
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 113x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/30/2017 11:14:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,722
            CDS (total)                       :: 5,599
            Genes (coding)                    :: 5,395
            CDS (coding)                      :: 5,395
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 90 of 204
            Pseudo Genes (incomplete)         :: 110 of 204
            Pseudo Genes (internal stop)      :: 52 of 204
            Pseudo Genes (multiple problems)  :: 41 of 204
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF322"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
     gene            complement(62..403)
                     /locus_tag="CTI52_28560"
                     /pseudo=""
     CDS             complement(62..403)
                     /locus_tag="CTI52_28560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086853.1"
                     /note="incomplete; partial on complete genome; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /name="mobilization protein"
     gene            complement(378..923)
                     /locus_tag="CTI52_28565"
                     /pseudo=""
     CDS             complement(378..923)
                     /locus_tag="CTI52_28565"
                     /inference="COORDINATES: protein motif:HMM:PF03432.12"
                     /note="incomplete; partial on complete genome; missing
                     start and stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /name="nuclease"
     gene            complement(1081..1425)
                     /locus_tag="CTI52_28570"
     CDS             complement(1081..1425)
                     /locus_tag="CTI52_28570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="ATQ91715.1"
                     /translation="MADKRSKMLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPAR
                     AGKLPSISPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            complement(1600..1840)
                     /locus_tag="CTI52_28575"
                     /pseudo=""
     CDS             complement(1600..1840)
                     /locus_tag="CTI52_28575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            1806..2018
                     /locus_tag="CTI52_28580"
                     /pseudo=""
     CDS             1806..2018
                     /locus_tag="CTI52_28580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001692121.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing start; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            2043..2213
                     /locus_tag="CTI52_28585"
     CDS             2043..2213
                     /locus_tag="CTI52_28585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="ATQ91716.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="replication initiation protein"
     gene            complement(2422..2754)
                     /locus_tag="CTI52_28590"
     CDS             complement(2422..2754)
                     /locus_tag="CTI52_28590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005969762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug SMR transporter"
                     /protein_id="ATQ91717.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="multidrug SMR transporter"
     gene            complement(2922..3137)
                     /locus_tag="CTI52_28595"
     CDS             complement(2922..3137)
                     /locus_tag="CTI52_28595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="ATQ91718.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="mobilization protein"
     ARG             complement(2423..2754)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          complement(1501..1692)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          1672..2155
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.756"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          complement(4..1091)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.939"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2825..3219)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.313"
                     /coverage="99.0"
                     /evalue="8.29e-62"
ORIGIN
        1 gctcgccgca ggtcgagagc tgtacgccgc tgttcctctc tgacagcatg gcctgcgtgc
       61 tcttctgctc ccggatggtc gtatagttct cgagtatctg ctgcccctgc caccacagaa
      121 tgcccgcgct cgacgctatc agcagcgcgg agaccagaac gatggtcagc cacgtctggc
      181 tgaccatgcg cagcatccct ttcgtgcgct ggctcatggc tgaggacagc ttccggtcgt
      241 ggtcgaggat ggcggcgctg attctcttct cgctttcgct cagttccgct ctgacagact
      301 tctcgtgctc gctgaatgcg gctttcagca tctcgccggt agtctgctgc tgcgctttcg
      361 atttctgctc taagtccttc gccagcgtta aaagactgtt catagatggc tcccttcagt
      421 cggatgtttc gccccccgtc cgggtcggca atgctgatgc tgctttttgt ggtgcgcacc
      481 acctcaaaac ctgcgctttc cagcgcctca gtgacgtcct gacgcgtttt cagctccccg
      541 gatgaggcaa gggcaagcag gccgctcgta atcgcctggg cggcttcctg cttcgcttcc
      601 ggcagcgcgg aaggcgagac cagcacccgc cggttctccg gcgcgttcgg gtcatgcagc
      661 cccagcctgc cgttcactat ggtctgccag gcatcgatgc gcggacggtc ggcgcggtcg
      721 taatacggct gtagccgctt gccggtcaga agctccgtgt tcgggatcag gaagttcagc
      781 tccagccgcc ccttgtcccg gtgctcaacc cacagcacgc tgtactggtc tttatcgagt
      841 ccgggcatca gaacccgctc gaaactcgcc atcagctttt cgcgctggcc gggcggtaaa
      901 tcctgttcgg caaaagacag aacgccggaa gtgtactttt tggcgtaggg cgaggcgtcg
      961 ataagctccc ggacttcatc cggcttcccc tgcagaacgc tggcgccgtc gcgctggcga
     1021 tctttaccca gcagataatc gaccggcccg ccgccgccgc cgcgcccccg aggatgaaac
     1081 ttaacgatca tcatcagcac ccttttccag tacggcatgc cgcaaccgct cgagtccggc
     1141 atcgatggcc atcagcgcgg cgacaatctg cacgcggtcg tgtccgcttc caccaccggc
     1201 gttaacctgg cgggcaatct ggttaaggtt gttccccatg ccggcaagct gacgaagcag
     1261 cgccggcgag atcgacggaa gtttgccggc gcgtgcgggc ttctcgtcca ggcacgtctg
     1321 ccgcatccag gccgcgagtt gcttaccctc gcagcgctcc agcagacgcc ggtgctcgtc
     1381 ctcagtcacc cacatggtga gcattttgct gcgtttgtct gccaatccgg cctcctgata
     1441 acgccgggac ggacgggaaa tcttttcccg gtcggcacgg tgttggacaa gccgcaggcg
     1501 cggcagtggt tttagcgggt ttcggggcgc agccctgaac cagtcacgta gcgctagcgg
     1561 agtgtatact ggctaaacat gttatgacgg agtggcaatt tatgaaagtg cttcatgtga
     1621 caggggaaaa atgcggctgc cgggcgtaag tgcaaaatgc aggtggtgat gaaatgacgc
     1681 ctcctcgctc actcggtcgc tacgctcctg ccgtgagact gcggcgggcg ttaccagctc
     1741 acagattacg ggataagcaa atgccgctcg aagcaggaaa ggccaagaaa aggatgcgga
     1801 gttgccgttt ttccataggc tccgcccccc tgacgagtat cacgaaaatg acgctcaaat
     1861 cagcggtggc gaaacccgat gcggcttaaa gataccaggc gtttccccct ggtggctccc
     1921 tcgtgctccc ccgcattcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1981 ggtttctcat tccacgcctg aaactcggtt ccgggtaggc agttcgctcc aggatggact
     2041 gtatgcacga accccccgtt cagtccgact accacgcccg ttccggtaac tatcaacttg
     2101 agtccaaccc ggaaagacac gacaaatcgc cagtggcggt agccattggt aactgggttc
     2161 acggagcaga gattgtagag aatgcctaca gagttcttga agtgaaagcc tgagtgcggc
     2221 tacactgaaa ggacagttta ggtatctgcg ctccgtgaaa gccagttacc atcggaacaa
     2281 agagttggta gctctgaatt ccggcaaacg aaccaccgtt ggtagcggtg gtttttttgt
     2341 ttacagggaa aaagattacg cgcagaaaga aaggatctca aggaaatcct tttgctttgg
     2401 ccaatatgct tcggtaaaaa gttaatgcgc agaagatttt gaaaaaatat taataaccaa
     2461 aactccaacg caaataagga acattcctgc aattgcgggc caatcaagtt tttgaccgct
     2521 aaccaaccaa ccagctaggc ttattagtac aattcccgca ccagaccaaa ttgcgtaagc
     2581 aataccggta ggtatatttt ccaatgtttg ggaaagcaga taaaaggcag cgcaataact
     2641 tattgcagtt cctacagacg gccacaggcg tgtaaatcct tctgaatatt tcataaaggt
     2701 ggtgcctatg acttcagcca caatagcccc tcccaggaaa atatatgtat tcataaaaat
     2761 acctcatgtt gaaaaattta aatatcttta cataacaact aattaataga acccggctta
     2821 tcatagcgaa ggaccatgat gctgccactg cgatcgttcc agctccagcg ctttctggtg
     2881 ctgcaccgcc cgctcctgct gccggtgagt ttctatcgcg ttcagccttt cgacaaccgg
     2941 cgcagctgct cgctcaaact ctgcacctgc ctgctcaaga ccgtgacgcg ctcgttcagc
     3001 gccgcgttct cccgttgaat aagaccagac atcgtccgcc attccgcgaa ggcgttctcc
     3061 cactcgtcca gccttttcga gtagtcctgc tgtagctgct ctaatgcgct cagcaactgt
     3121 ttttccagct ccgtcatgtg ctatttcccc gccagtatca tccagctcga gtcctctccg
     3181 aactgtcccg cttccgggtt caccctcacg cagcggcgtc tct
//
