LOCUS       CP024486                3514 bp    DNA     circular BCT 06-NOV-2017
DEFINITION  Klebsiella pneumoniae strain INF322 plasmid unnamed4, complete
            sequence.
ACCESSION   CP024486
VERSION     CP024486.1
DBLINK      BioProject: PRJNA351909
            BioSample: SAMEA3357193
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: INF322
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 113x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/30/2017 11:14:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,722
            CDS (total)                       :: 5,599
            Genes (coding)                    :: 5,395
            CDS (coding)                      :: 5,395
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 90 of 204
            Pseudo Genes (incomplete)         :: 110 of 204
            Pseudo Genes (internal stop)      :: 52 of 204
            Pseudo Genes (multiple problems)  :: 41 of 204
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF322"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
     gene            179..537
                     /locus_tag="CTI52_28535"
                     /pseudo=""
     CDS             179..537
                     /locus_tag="CTI52_28535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532398.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            601..732
                     /locus_tag="CTI52_28540"
     CDS             601..732
                     /locus_tag="CTI52_28540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="ATQ91714.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGNRISRAS"
                     /name="replication initiation protein"
     gene            1152..1343
                     /locus_tag="CTI52_28545"
     CDS             1152..1343
                     /locus_tag="CTI52_28545"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATQ91711.1"
                     /translation="MLKKNEIILIKSCGNSKTKIFLRGVDEGLMIDASFEDIKFAINNN
                     LHDLLGIQAETTKLKLTK"
                     /name="hypothetical protein"
     gene            complement(1638..3164)
                     /locus_tag="CTI52_28550"
     CDS             complement(1638..3164)
                     /locus_tag="CTI52_28550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="ATQ91712.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQREGASVLQGKPEEVRELID
                     ASPYVKKYTSGVLSFAEADLPPGQREKLMASFERVLMPGLDKDQYSILWVEHADKGRLE
                     LNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTVVNGRLGLHDPNDPENRRALVTPSGL
                     PETKQEAAETITRGLLMLASSGELKTRDDVTGALTAAGFEVVRTTKNSISIADPDGGRN
                     IRLRGAIYEQSFNGLEGLRGEIESTAAAYRREAESRIQQARERCQIGTERKREENQRRH
                     QRPRTGDERGDAVEPAERAANGRADVADHHHGVRAAVRQPERGAVVPGEPDSGEPGGNQ
                     AAERYAVETAHADVGRDLSGEPQRTVSGAAGGDESRNGPDGGQQDAERGEAGTGVEQND
                     GTGKTIAERVRAATAGLLEKAGRVGERLRGMAEDVWSYATGERGAERARVGLEQAGAEF
                     ERAAAPVIDRLNEIEQQRYGQTTEKEPEKTADLVPERQRTLSHGDREYGD"
                     /name="nuclease"
     gene            complement(3154..3495)
                     /locus_tag="CTI52_28555"
     CDS             complement(3154..3495)
                     /locus_tag="CTI52_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="ATQ91713.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="plasmid mobilization relaxosome protein MobC"
     Typing          complement(67..239)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.655"
                     /coverage="100.0"
                     /evalue="9.54e-56"
     Typing          164..710
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.909"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(1642..3164)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3161..3474)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.37e-58"
ORIGIN
        1 cgtgacggac gggaaagcct ttcccggtcg gcgcggggtt ggacaagccg caggcgcgtc
       61 agtggggttt cggggcgcag ccctgaacca gtcacgtagc gctagcggag tgtatactgg
      121 cttagtatgt tggcatgagt gcgataatca ggaaagcgcc ttatgcggca gaagaaaaat
      181 gctgcagcag agcgtaagca gaatatgcga tacagaagat attccgcttc ctcgctcact
      241 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtgc cggcttacgg gcggggcgga
      301 aatttccagg aagataccag gaagaaactt aacagggaag cgatgaggcc gcggcgaagc
      361 cgtttttcca taggctccgc ccccctgaca agcatcacaa aatctgacgc tcaaatcagt
      421 ggcggcgaaa cccgacagga ctataaagat cccaggcgtt tccccctggt agctccctcg
      481 tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt
      541 tatctcattc cacgcctgac actcagttcc gggaaggcag ttcgctccaa gctggactgt
      601 atgcacgaac cccccgttca gtccgactgc tgcgccttat ccggtaacta tcgtcttgag
      661 tccaacccgg aaagacacgc caaagcgcca ctggcagcag ccactggtaa caggattagc
      721 agagcgagtt aatgtaggtg atgctacaga gttcttgaag tggtggccta actacggcta
      781 cactggaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct ggttaagcag
      841 ttccccaact gacttaacct tcgaaaaacc gccctgcaag gcggtttttt cgtttacaga
      901 gcaagagatt acgacgaggc tgaatgaatc tcaagatttc atttagcctg ccacccgatc
      961 attcaaagtt ttggcgtagt tctgacccgc tccccgtgat ttgttacata cccaccacag
     1021 ggcattagtt tttttatttt tttgatgata tgtacaaaat cagtggttag gaataatctt
     1081 agcagcctta acaaacacac acaaaaacaa cgataatgat tcatctgacg acaacaaaca
     1141 atgacaatgt catgttaaaa aaaaatgaaa ttatcttaat aaaatcatgt ggtaactcaa
     1201 aaacaaaaat ttttttacga ggtgtggatg agggactcat gattgatgca agttttgaag
     1261 atataaaatt tgctatcaac aataacctcc acgatcttct cggaatccaa gccgaaacca
     1321 caaaactaaa actaacaaag tgatacagcc cctgaagcat gaaggatcga aataaagcca
     1381 ccgatttacc ataacgtctt cttacccgca ccgcgccgga ggcactcagg gcgtttctga
     1441 tggctgacgg gcaaaaggtg gtggcaagta caccgaaaac caccagaaac gcacctgatt
     1501 tattttacgc atatgcatca cttattcaca tcactgcata gccggtgatt tcggttaaaa
     1561 aagactctct gtgtgctgaa atcgcagaaa atggccatga tccccgtcac gctcaggcca
     1621 cctgagacgg gcgtttatca gtctccatac tccctgtcac catgagataa tgtgcgttgc
     1681 cgttccggta ccagatctgc tgtcttctct ggttcctttt ccgttgtttg cccgtatcgc
     1741 tgttgctcaa tctcattcag cctgtcgata accggcgcag ctgctcgctc aaactctgca
     1801 cctgcctgct caagcccgac acgcgctcgc tcagcgccgc gttctcccgt tgcataagac
     1861 cagacatctt ccgccattcc tcgaaggcgc tctcccactc gtccagcctt ctcgagtagt
     1921 cctgctgtag ctgctctaac gcgctcagca attgtttttc cagttccgtc attctgctct
     1981 actcccgtac cagcttcacc gcgttccgcg tcttgttgtc caccgtccgg cccgtttcgg
     2041 ctttcatccc ctccggcagc accagaaacc gtccgttgcg gttctccaga taggtcacgc
     2101 cccacgtctg catgtgcagt ttcgacagcg tatctctctg ctgcctgatt tccgccaggt
     2161 tcgccgctat ccggttcccc tggtaccaca gcaccccgct caggctggcg aacagcagcc
     2221 ctgacaccat ggtgatggtc agccacgtcc gcccgaccat tcgcagcacg ctcagccggt
     2281 tcgactgcat cgccgcgttc atcccctgtt cgtgggcgct gatggcgtcg ttgattctct
     2341 tcgcgcttgc gttcagttcc gatttgacaa cgttctcgtg cttgctgaat gcggctttca
     2401 gcctctcttc ggtaagctgc tgctgtgctt tcgatttctc ctcgaagtcc ctcgaggccg
     2461 ttaaaagact gctcataaat ggctcccctc agtcggatat ttcgcccccc gtccgggtcg
     2521 gcaatgctga tactgttttt tgtggttctg accacctcaa aaccggccgc tgtgagcgcc
     2581 cctgtgacgt catcacgcgt tttcagctct cctgatgagg ctaacatcag caaaccccgt
     2641 gtaatcgtct ctgcggcttc ctgcttcgtt tctggcagtc ctgaaggcgt gaccagcgcc
     2701 cgccggttct ccgggtcgtt cggatcatgt agccccagcc tgccgttgac cactgtctgc
     2761 cacgcatcga tgcgcggccg gtcggcacga tcgtaatacg gctggaggcg tttccccgtc
     2821 agtaattcgg tattggggat cagaaaattc agctcaagac gacccttgtc tgcgtgttcc
     2881 acccacagaa tgctgtactg gtctttatcg agtccgggca tcagaacccg ctcaaagctg
     2941 gccatcagtt tttcacgctg acccggcggc aggtccgcct ccgcgaatga cagcaccccg
     3001 gaggtatatt ttttcacata cggcgacgca tcaatcagct cccggacttc ttccggcttc
     3061 ccctgcagga cgctggcacc ttcacgctgc cggtctttac ccagaagata atccacagga
     3121 ccggcgcccc cgccgcgacc ccggggatgg aatttaacaa tcatcgtccg gccctttttc
     3181 cagtacggca tgccgcaacc gttccagacc ggcatcgatc gccatcagcg ctgccacgat
     3241 ctgcacccgg tcatgaccgg caccaccggc gttcactttc cgggcgatct ggttcaggtt
     3301 attgcccatg ccggcaagct gacgcagcag cgcgggcgaa atcgagggaa gcctgccggc
     3361 gcgtgtcggt ttctcatcaa ggcaggtctg gcgcatccac gccgccagtt gttttccgtc
     3421 gcaccgctca agcaaccgcc ggtgttcgtc ttccgtcacc cacatcgtga gcatcttgtt
     3481 gcgtttatct gccagaatcg cctcctgata acgc
//
