LOCUS       CP023936                5596 bp    DNA     circular BCT 30-SEP-2019
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_443 plasmid unnamed5, complete
            sequence.
ACCESSION   CP023936
VERSION     CP023936.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN07312487
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Croxen,M., Tallon,L.J., Sadzewicz,L., Ott,S., Zhao,X., Nagaraj,S.,
            Vavikolanu,K., Aluvathingal,J., Nadendla,S., Geyer,C. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Croxen,M., Tallon,L.J., Sadzewicz,L., Ott,S., Zhao,X., Nagaraj,S.,
            Vavikolanu,K., Aluvathingal,J., Nadendla,S., Geyer,C. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2017) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0, HGAP v. 3, SPAdes v. 3.6.0
            Genome Coverage                   :: 636.834x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/11/2017 17:24:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,168
            CDS (total)                       :: 6,048
            Genes (coding)                    :: 5,790
            CDS (coding)                      :: 5,790
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 117 of 258
            Pseudo Genes (incomplete)         :: 140 of 258
            Pseudo Genes (internal stop)      :: 54 of 258
            Pseudo Genes (multiple problems)  :: 49 of 258
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_443"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Canada: BC"
                     /collection_date="Nov-2012"
     gene            complement(33..179)
                     /locus_tag="CRN73_28485"
                     /pseudo=""
     CDS             complement(33..179)
                     /locus_tag="CRN73_28485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063820.1"
                     /note="incomplete; partial on complete genome; missing
                     start and stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /name="mobilization protein"
     gene            complement(154..699)
                     /locus_tag="CRN73_28490"
                     /pseudo=""
     CDS             complement(154..699)
                     /locus_tag="CRN73_28490"
                     /inference="COORDINATES: protein motif:HMM:PF03432.12"
                     /note="incomplete; partial on complete genome; missing
                     start and stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /name="nuclease"
     gene            complement(857..1201)
                     /locus_tag="CRN73_28495"
     CDS             complement(857..1201)
                     /locus_tag="CRN73_28495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="ATM57037.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            complement(1552..1782)
                     /locus_tag="CRN73_28500"
                     /pseudo=""
     CDS             complement(1552..1782)
                     /locus_tag="CRN73_28500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347451.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            2470..2849
                     /locus_tag="CRN73_28505"
                     /pseudo=""
     CDS             2470..2849
                     /locus_tag="CRN73_28505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198370.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            2875..3420
                     /locus_tag="CRN73_28510"
     CDS             2875..3420
                     /locus_tag="CRN73_28510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATM57038.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            3486..3836
                     /locus_tag="CRN73_28515"
     CDS             3486..3836
                     /locus_tag="CRN73_28515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATM57039.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(3906..4172)
                     /locus_tag="CRN73_28520"
     CDS             complement(3906..4172)
                     /locus_tag="CRN73_28520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="ATM57040.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4156..4380)
                     /locus_tag="CRN73_28525"
     CDS             complement(4156..4380)
                     /locus_tag="CRN73_28525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="ATM57041.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="TraY domain-containing protein"
     gene            complement(5047..5259)
                     /locus_tag="CRN73_28530"
     CDS             complement(5047..5259)
                     /locus_tag="CRN73_28530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="ATM57042.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="mobilization protein"
     gene            complement(5266..5589)
                     /locus_tag="CRN73_28535"
     CDS             complement(5266..5589)
                     /locus_tag="CRN73_28535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="ATM57043.1"
                     /translation="MQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIASNL
                     AEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNALRL
                     VKE"
                     /name="mobilization protein"
     Typing          complement(1454..1530)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          1719..2116
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(2..867)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="86.0"
                     /evalue="0.0"
     Typing          complement(4932..5596)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.55"
                     /coverage="100.0"
                     /evalue="3.0000000000000003e-117"
ORIGIN
        1 ctcataccct gctcgtgggc gcggatggcg gcgctgattc tcttcccgct ttcgctcagt
       61 tccgctctga cagacttctc gtgctcgctg aatgcggctt tcagcatttc gccggtactc
      121 tgctgctgcg ctttcgattt ctgctctaag tccttcgcca gcgttaaaag actgttcata
      181 gatggctccc ttaagtcgga tgtttcgccc tccgtccggg tcggcaatgc tgatgctgct
      241 ctttgtggtg cgcacgacct caaaacctgc gctttccagc gcctcagtga cgtcctgacg
      301 cgttttaagc tcccctgacg aggcgagggc cagtaagccc cgcgtaatag cctctgcggc
      361 ttcctgcttc gttttgggta acgaagatgg cgtcaccagc gcgcgccggt tctccggtgc
      421 gttcgggtcg tgcagcccca gtcgcccgtt caccacggtc tgccacgcat cgatgcgcgg
      481 acggtctgcc cggtcgtagt acggctggag acgcctgccg gtcagcagtt ccgtgttcgg
      541 taccagaaaa ttcagttcca gccgcccctt gtcccggtgt tcaacccaca gcacgctgta
      601 ctggtcttta tcgagtccgg gcatcagaac ccgctcgaag ctcgccatca gcttttcacg
      661 ctggccgggc ggtaaatcct gttcagcaaa agacaggacg ccggaggtgt actttttggc
      721 gtagggcgag gcgtcgataa gctcccggac ctcctccggc tttccctgca ggacgatggc
      781 cccgtcgcgc tggcggtctt tccccagcag ataatccacc ggcccggcac cgccgcctcg
      841 ccctcgcgga tgaaacttaa cgatcatcgt ctgccccctt ttccagcacg gcatgccgca
      901 gccgctcgag ttcggcatca atggccatca gcgcggccac cacctgaacc cggtcagcgc
      961 ctgaccactg gccgccgtta attttccggg cgatctggtt gaggttgttc cccatcccgg
     1021 caagctggcg cagcaaaacc ggatcgatgg acggcagccg caacgaccgc gccgggcgcg
     1081 tatcgagaca ggtttcccgc atccacaccg ccagttgcct tccattgcag cgctccagca
     1141 gctgctggta ctccccatcg gtcacccgga tacctaaaaa acgggtacgc ttaaccgcca
     1201 tcctctcctc cgatatccgc aaaatgtgac cgggctgaac gctcaagctc tgccatctgc
     1261 tcccggcggt aatcttcctc ctgtgcctga cgcatcagat ttgccatcac tgccggatgc
     1321 caggatcttt tttttacgac gagcggcttc ttcttcggct tctgcattat ccctccttcg
     1381 caaacgccgg acggcacgga aaagttttcc gggtcgctcc ggcgttggac aagcgccagc
     1441 gcgtcagtgg tgttagcggg tttcggggcg cagccctgaa ccagtcacag cgtttacgct
     1501 gtgtatactg gcttactatg ttggcatgag agcacgattc atgaaagtgc ctcatgtggc
     1561 aggtgtgata tgtggctcag gagcgtccgc aataccatgc acggacagat gaaatgacgc
     1621 ctcctcgctc actcggtagc tgcgctcctg ccgttcggct gcggcgggcg tgaccgactc
     1681 acggataacg ggataaagca aaggcgctca aatcagaaag ggccggagaa aaggatgcgg
     1741 cgttgccgtt tttccatagg ctccgcctcc cctgacgagc atcacagaaa tggacgctca
     1801 agtcagagga ggacgaaacc cgacaggact taaagatgcc ggtgatttct ctccggcggc
     1861 tccctcgtgc gctctcctgt tccgaccctg ccgtttaccg gatacctgtt ccgccttttt
     1921 ccctttcggg aagcgtggcg ctttctcata gctcacgctg ctggtatctc ggctcggttt
     1981 aggtcgttcg ctccaagctg ggctgtatgc acgaaccccc cgttcagccc gactgctgcg
     2041 ccctttccgg taactatcat cttgagtcca acccggacgg acacgcaaaa acgccactgg
     2101 cagcagccat tggtaactgg atatatacga gagagatatt gagaggtctt gaagtgggtg
     2161 cctaactgcg gctacactga aagaacagtt ttggtatctg cggtctcgta tacccagtta
     2221 cccggttaag cagttcccca actgacttaa ccttcgatca aaccgcctcc ccaggcggtt
     2281 ttttcgttta cagtgcaaga gattacgcgc agaaaaaaag gatctcaaga agatccttta
     2341 aatgatcgtc agttctgtta tccccggagc tccatccatc ccacggaaaa acacccgcca
     2401 ctgatgccct cagcgcactg cacatcatgt acaaaccact ccctccctcc ttttttattg
     2461 cgtttcttaa tgcgcaatgg tagctttgtg cgcaacaaaa aacgcaatag caaggggggg
     2521 taatgagaga aagaacaaat atcatgctag atataggatt aaagcaatcc ttacaacggc
     2581 tttcgaataa aaccaaaatg agtctaagtg atcttattaa ccagagatta tcaggttcac
     2641 tttcagttga agaacgcatt ggcgaaaagc ctgaatggat gagaaacctt gatccctatc
     2701 tcgccaatca cttttcaaat attgagttac cgctcccttc accattaaaa gaataccaag
     2761 aaaaaatgga tgagatagat tcaattatta atgacatgat ggttctaaag gaatatttca
     2821 gagctaagtt ttacgacgaa ataaaatagg cacgctatac ggaggcggag aattatgttt
     2881 tttaaacggt atttaacgga gaaggaaata tacaaggcag cactcccaat tgttaggctt
     2941 caagggagag ttaacacttc attacgtttt ttatccaaat actcagatgt aaacaaaaat
     3001 ggattaattg cattgcatct taatttatta cattttatct catacaacat acacggtgta
     3061 gagtctctga aaatcactca agcaggcata aacaaagctg tacaacaaag ccctcacgca
     3121 gatgaaataa aaaatattgc gcaggcaata ggacgcaaag gacctgagta catcgaagga
     3181 ttaattagaa acccatgggg gaagaaaaat gataatccac taatatttat aatgagcatt
     3241 gaagaaagta taaaagaaac ccttaactca ggttatatgt tatatagagt agctcccata
     3301 actgagggtg aagcagctgc tttcatggat gttcttaaac aattattatt atttgaggat
     3361 aattttcgcc tttttggtag agacttggtt tataaaatag aaaaattaaa aatcagataa
     3421 caaagtatgg tgaacgaaaa atgaaagaga ggatagtgaa atgtgtaata ccaagattct
     3481 caactatgta ttggttaatt gcgttcctag catttatatt ccccggtcga tcttttgctg
     3541 acggttacac cgcttactac cgatgtaatg gtactcagga agcatctctt actgttaaag
     3601 ataacaaact aagtatggtt gtaggtcttt ctaagtttaa taggcaactg gatacttata
     3661 gattcaaaga aaagggaatc gaagtagtaa tgcagagagc tgcttctgat gataactctg
     3721 aggccataat gtcttttatt atgcctgacg ttaacaatat gaccttatta gttaaaaata
     3781 acggctacat tatcgttaag acaacctgct ggttaactaa aaccgacaaa cagtagaaca
     3841 cgtagttagt cgagtattta ccataatacc tgttgtcggc atcaaaaaag cccgcatagc
     3901 gggcttcagt cgtaaacttc gcggcgatgt ccaatcgtgg cggcaaggat aacaagctcg
     3961 tcatctcgga tctcacacag aacgcgataa tctcccaccc gatagcgcca gaactcgccc
     4021 agctcacctt tgagcggctt ccctgactgg cgaggatcgg cagcaactgc aatgcgtaaa
     4081 ctcataaaat ccacaatccg tcgtgcgttc tgtttgtcca tcttgcgtaa cgatttgagc
     4141 gcccgatcgg aatagttaat cgtccagacc aagtgacttc ctcacgtctt cagacgaatg
     4201 cactgcttca tcaccacggc gaacgcgtgc agcagtttct gctgaaagat aataatcctc
     4261 caggtcttcc agatgcgcca gtattgcttc ccgcgcataa aacgtctttg ttctgccggt
     4321 ttgcttcgcc agcgagtcca gacgggactc aatctcatca gataaccgga tagcaagcat
     4381 ataaacctcc cgaacaccat ttgctataca tgtatatcaa cagtaccaca atcaacctaa
     4441 aagaacaaaa aataatcttg ttagcccctc ttgataatcc gtaacgcggt caatatatct
     4501 agcttgaggg ctttcctctt aactattgaa aggataacga ttatgggtct taagccgggt
     4561 ccgaaacgaa ttgccgattc aacaggggaa cctgataaac gccagcgtga caataaaaaa
     4621 acgccgggaa atactgacaa attgaaaccc agcaaatctt caaaaaaata agtcagtagt
     4681 taaaagccct ccggggcttt tttgtatctg ccgttcacgc tgattaaaca taacacccgt
     4741 tgcccgcacc gccccggacg ggttcgccgc gaacggggtg gcagtcaggt aaaaccggca
     4801 acaaaccacc cgataaaccg ccccgttcgc gacagataaa ttttacctgg ctgcatagcc
     4861 atgcagccag gtgaatgaca tccggtttca gcgtaaatgc ccgatgagaa ccctgttttc
     4921 ctgcatttca gcccagcgac ggaccgtcat agcttcgctg ccgggaacgc gcttccgtca
     4981 gttccttttc gtgctgccgt tgctcgtgcg cccgttctgc ggccacggcc atctcatgac
     5041 gctgcactac cggctcaaac gctgcagttg cccgctcaag cgttccacct gctgactcaa
     5101 gcgcgtcacg cgctcgctca gcgccgcgtt ctcccgttgc ataagccctg acattttccg
     5161 ccattccccg aaggcgctct cccactcgtc cagccttttc gagtagtcct gctgtagctg
     5221 ctctaatgcg ctcagcaact gtttttccag ctcggtcatc ggcacttact ccttcaccag
     5281 tctcagcgcg ttctgcttcg accgcccgtt cccgaccgtc cagctctgcg tcctgtctac
     5341 ggccgtcccc ttcggcagca ccagaaaacg tccgttgctg tcttccaggt acgtcacgcc
     5401 ccaggtcttc gcgttcagct tcgacagcgc tgcgttctgc cggtcgattt cggccagatt
     5461 tgacgctatc aagctcccct gataccacaa caccccgctc aggctggcga acagcagtcc
     5521 tgacaccatc gtgatggtca gccatgtccg cccgaccata cgcatcacgc tcaggcggtt
     5581 cgactgcatg gccgcg
//
