LOCUS       CP023932               11972 bp    DNA     circular BCT 30-SEP-2019
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_443 plasmid unnamed4, complete
            sequence.
ACCESSION   CP023932
VERSION     CP023932.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN07312487
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11972)
  AUTHORS   Croxen,M., Tallon,L.J., Sadzewicz,L., Ott,S., Zhao,X., Nagaraj,S.,
            Vavikolanu,K., Aluvathingal,J., Nadendla,S., Geyer,C. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11972)
  AUTHORS   Croxen,M., Tallon,L.J., Sadzewicz,L., Ott,S., Zhao,X., Nagaraj,S.,
            Vavikolanu,K., Aluvathingal,J., Nadendla,S., Geyer,C. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2017) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0, HGAP v. 3, SPAdes v. 3.6.0
            Genome Coverage                   :: 636.834x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/11/2017 17:24:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,168
            CDS (total)                       :: 6,048
            Genes (coding)                    :: 5,790
            CDS (coding)                      :: 5,790
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 117 of 258
            Pseudo Genes (incomplete)         :: 140 of 258
            Pseudo Genes (internal stop)      :: 54 of 258
            Pseudo Genes (multiple problems)  :: 49 of 258
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11972
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_443"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="Canada: BC"
                     /collection_date="Nov-2012"
     gene            complement(join(11813..11972,1..86))
                     /locus_tag="CRN73_00005"
     CDS             complement(join(11813..11972,1..86))
                     /locus_tag="CRN73_00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATM51653.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            483..734
                     /locus_tag="CRN73_00010"
     CDS             483..734
                     /locus_tag="CRN73_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012600009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATM51638.1"
                     /translation="MTGVLTGQPLSHEMYIKLPGADVVLLTEGNIIGCDTASATWTRRG
                     LRPWHVNTISTREPGDLPSDHLPVSEMARTGKTRSHSR"
                     /name="hypothetical protein"
     gene            734..2218
                     /gene="ltrA"
                     /locus_tag="CRN73_00015"
     CDS             734..2218
                     /gene="ltrA"
                     /locus_tag="CRN73_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="ATM51639.1"
                     /translation="MYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRTQ
                     SRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMDY
                     AGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKILN
                     AVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIRF
                     TEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWAH
                     QWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEFG
                     RFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLRR
                     RWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTTW
                     TKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            2323..4008
                     /locus_tag="CRN73_00020"
     CDS             2323..4008
                     /locus_tag="CRN73_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="ATM51640.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            4018..4275
                     /locus_tag="CRN73_00025"
     CDS             4018..4275
                     /locus_tag="CRN73_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="ATM51641.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="cloacin"
     gene            4360..4509
                     /locus_tag="CRN73_00030"
     CDS             4360..4509
                     /locus_tag="CRN73_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="ATM51642.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="lysis protein"
     gene            4761..5081
                     /locus_tag="CRN73_00035"
     CDS             4761..5081
                     /locus_tag="CRN73_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATM51643.1"
                     /translation="MAAASGVLRAFPGWTQVNSYRIRRSSRAERGVLAYSPAWSERPTP
                     SRDTSCVSYEKAPHFPQGRTAEQVSGKRQGRNRRAQEGATYRKRWGSLSPVGFRPYCQI
                     HG"
                     /name="hypothetical protein"
     gene            5662..5967
                     /locus_tag="CRN73_00040"
     CDS             5662..5967
                     /locus_tag="CRN73_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATM51644.1"
                     /translation="MLDAPDEAAKLLLILRVVSRSLWRTLTGAGTPEKTALGCRLFLSH
                     AGACSLFLRGCAFSAWGGKRLASAVKAERNHHGRDRRRHALGHVRGLCSGGCASRR"
                     /name="hypothetical protein"
     gene            5995..7959
                     /locus_tag="CRN73_00045"
     CDS             5995..7959
                     /locus_tag="CRN73_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mobilization protein A"
                     /protein_id="ATM51645.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="Mobilization protein A"
     gene            7959..8690
                     /locus_tag="CRN73_00050"
     CDS             7959..8690
                     /locus_tag="CRN73_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATM51646.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="hypothetical protein"
     gene            8697..9227
                     /locus_tag="CRN73_00055"
     CDS             8697..9227
                     /locus_tag="CRN73_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATM51647.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(9254..9433)
                     /locus_tag="CRN73_00060"
     CDS             complement(9254..9433)
                     /locus_tag="CRN73_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="ATM51648.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(9459..9887)
                     /locus_tag="CRN73_00065"
     CDS             complement(9459..9887)
                     /locus_tag="CRN73_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATM51649.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(10051..10365)
                     /locus_tag="CRN73_00070"
     CDS             complement(10051..10365)
                     /locus_tag="CRN73_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="ATM51650.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(10353..10631)
                     /locus_tag="CRN73_00075"
     CDS             complement(10353..10631)
                     /locus_tag="CRN73_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019952150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="ATM51654.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="ribbon-helix-helix protein, CopG family"
     gene            complement(10806..11171)
                     /locus_tag="CRN73_00080"
     CDS             complement(10806..11171)
                     /locus_tag="CRN73_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ATM51651.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="hypothetical protein"
     gene            complement(11168..11539)
                     /gene="tolA"
                     /locus_tag="CRN73_00085"
     CDS             complement(11168..11539)
                     /gene="tolA"
                     /locus_tag="CRN73_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolA"
                     /protein_id="ATM51652.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     BGC             1..9008
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4613..5193)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.285"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7960..8687
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 cctttcaggc ccatggccag tgcctgagcg acaaaggcgt ccgccaggcg cttttcttca
       61 gcgggagtca gtttcaggtg cgtcatagtg gcctccgggc gtctcaggtt tcccgaacag
      121 tttaaccgca ggtcctctcc tcatgccagt tagctttcag atgtgtctat accatctgaa
      181 atgtaagtgt gatgaatgtc ttaaataata aaatttgttg ccatgagaaa ttttcgggaa
      241 aagacgcgat ctgcttacag gtggctccgg gcgctttttt gtgcctgaag atgagaacta
      301 atctcatata ttgacacctg aaaacgggag gagtaaggta ataattatac tgtgtatatg
      361 tacagtgtgc gccgggagac cggttagaga tcaaggggtg aaagtccccg accattgaag
      421 gaccagcaat ccacaaggtc cccgagtcat gcgttgcata ccgcgaggta tggggcgaag
      481 cgttgacagg ggtgttgaca ggccagccat tgagccacga aatgtatatt aaattaccgg
      541 gtgccgacgt tgtactgtta acggaaggca acatcatagg gtgcgatact gcgagtgcca
      601 catggacccg gcggggtctg agaccctggc atgtcaatac gatctctacg cgggaaccgg
      661 gagatctccc ctctgaccat ctgccagtgt cggagatggc ccgcaccggg aagacgagga
      721 gtcatagccg gtgatgtacg gagaggagaa gtcggactcg ctcatagtag cggcgaagca
      781 ggcgaacaac ccgaaaggag cggagtcagt ggagcgaagg agcggggcca aggggaacgc
      841 ggaacagcca cacatgcgcc ggacacagag ccgggaaagc atgtcacaga ggctgtcacg
      901 cgtgcgggaa gctgcgaagc agcggaagaa agaacggttt acagcattgt tccacctgct
      961 gacagtcgaa gcactggaag ccgcattcct ctccctgagc aggaaagcgg ccgccggagt
     1021 ggatggcatc aggtggatgg actacgccgg aaacatgaag aacaacataa cagatctgca
     1081 ccggaggcta catcagggca gctacagggc gcagcccggc aggcgtcact acatcccaaa
     1141 agcggatgga aaacaacgcc cgctcggcat cgcctcgctg gaggacaaga tcgtccagta
     1201 tgcgctggtg aaaatcctga acgcagtcta tgaaaacgac tttatggggt tctcatacgg
     1261 gttcagaccc gggcgaagcc agcacgatgc actggacgca ctggccacag ggctggtacg
     1321 cactaacgta aactgggtac tggatgccga catcagtcag ttcttcgaca gggtgagcca
     1381 cgaatggctg atcaggttca cagagcatcg gatcggcgac cggagggtaa tcaggctcat
     1441 acgtaagtgg ctcacagccg ggacgtcgga ggagggtcaa tggcgagcaa cggaggaagg
     1501 caccccacag ggtgcggtca tctcaccgct gctggcaaac atatacctcc actacgtctt
     1561 cgatctgtgg gcgcatcagt ggcgacgtcg ctatgccaca ggcaatgtgg taatggtcag
     1621 atacgccgat gacatcgtca tcgggttcga caaacgatac gatgcccggc gcttccgtat
     1681 agccatgcag cgcagactga gggagttcgg actcacggtt cacccggaga aaacccgtct
     1741 gatggagttc ggccgcttcg ctgccgaaaa ccgtgccatc aggggaaaag gcaaaccaga
     1801 aacgttcaac ttcctcgggt tcacgcacat cagcgggaaa gatcgcaacg gcaggttcat
     1861 gctgatacga aagacccgcc gggatcggat gacggcaact ctgaaagcca tcaaagacgg
     1921 tctgcgaagg cgctggcatt actcaatccc cgaacaggga aaatggctca ggagagtggt
     1981 tcagggatac ctgaactatc actcggtacc gggcaacttc cccaccatgc agaagttcag
     2041 gacacacgta acaaacctct ggcgccgggc gctcaggcgc aggagccaga aggatgatac
     2101 gacctggacg aaagcaaaca aactggcagc cgcatggcta ccaagggttc gggttcttca
     2161 tccatggcct gtggagcggt tcaccgccag acacccgagg caggagcccg gtgcgtaaat
     2221 cgcgcacgcc gggatctgtg cggggggtat ccggtaacgg gtatccctac cgcgacatgt
     2281 tggctttaat ttattgtttt aaatgtcaaa gaggaaaacg gtatgagtgg tggagacggt
     2341 cgaggtccgg gtaattcagg tctgggacat aatggtggtc aggccagtgg gaatgtgaac
     2401 ggtacgtctg gtaaaggtgg cccttcatca ggtggtggta cggatccaaa cagcgggccg
     2461 ggctggggta cgacgcatac gccgaatgga gatattcata actataatcc gggggagttt
     2521 ggtaacggcg ggagtaaacc cggtggtaat ggtggtaaca gcggcaatca tagcggtagc
     2581 tctggtggcg gacagtcttc ggccactgcg atggccttcg gtctgcctgc cctggctact
     2641 ccgggggctg aaggactggc tttatcagtt tccggcgatg cgttgtcggc cgccgttgct
     2701 gatgtgctgg ctgccctgaa ggggccgttt aagtttggtc tgtgggggat tgcgatctac
     2761 ggtgtgctgc cttctgagat tgcaaaagat gatccgaaaa tgatgtcaaa aattatgacg
     2821 tcattaccgg ccgatgcggt gacggagact ccggcaagta ctttaccact ggaccaggcg
     2881 acggttcgtg tcagacaacg ggttgtggat gtggtgaagg atgagcggca gcatattgcg
     2941 gttgtcgcag gtcggccaat gagtgtccct gtggtggatg cgaaaccgac aaaacgtccg
     3001 ggggtattca gtgtgtcgat tccgggtctc ccgtctctgc aggtgagcgt accgaaaggt
     3061 gttccgacag cgaaagcccc gccaaaaggc attgttgctg aaaaaggtga ttcacgtccg
     3121 gctggtttta cagccggtgg taactcccgt gaggccgtta ttcgtttccc gaaagagacc
     3181 ggacagaagc cggtttatgt gtcggtgaca gatgttctta ccccggcaca ggtaaaacag
     3241 cgtcaggagg aagaaaagcg tcgccagcag gcatgggacg ccgctcatcc ggaagagggg
     3301 ctgaaaagag actatgataa agcgaaagct gagctggatg ccgaagataa aaatattgcg
     3361 accttaaaca gccgcattgc atcgacagag aaggcgctcc ccggtgcaag ggctgctgtt
     3421 caggaagccg ataaaaaggt aaaagaggca gaggcaaata aagatgattt tgtgacttat
     3481 aacccgcctc atgaatatgg ctccgggtgg caggatcagg ttcgctatct tgataaggat
     3541 attcagaatc agaatgagaa attaaaagcg gctcaggcat ctttaaacgc aatgaatgaa
     3601 tccttatcca gagataaggc tgcacttacc ggggcgatgg agagccggaa acaaaaggag
     3661 aaaaaagcga aggatgcaga aaataagtta aatgaggaaa agaacaagcc tcgcaagggg
     3721 actaaagact acggccatga ttatcatcca gtacctaaaa cagaagacat aaagggactt
     3781 ggagagttaa agcgaggtga tcctaaaaca cctaaacaag gtggtggtgg taagcgggcc
     3841 cgatggtatg gcgataaaaa acgtaaaatt tatgaatggg attcccagca cggtgagctt
     3901 gaagggtatc gtgccagtga tggcgaacac ctaggcgcat ttgatcccaa aacgggtaag
     3961 caggttaaag ggccagatcc aaaacggaac attaaaaaat atctttgaga ggtaaatatg
     4021 gggcttaaat tacgtttgac atggtttgat aagaaaactg aagagtttaa aggggaagag
     4081 tattctaaag actttggtga cgatggttct gtcattgaaa gcttgggaat gcctttaaag
     4141 gataatatta ataatggtga gtttgatttg aagaaagaat gggttccttt actacagcca
     4201 ttttttaaaa atcaaataaa acctgatgtg gataattgtt atatagcttt tgattatcgg
     4261 gacggtaact ggtaacaaaa aattatcaag gatgatttga aatactgact tccggtgttc
     4321 agggatgaat gccggagtct ttaaatacag ggaaagtata tgaaaaaggt aaagactatt
     4381 tttcttttta tattgattgt tgctggcttt cttctggccg catgtcaggc aaattatatc
     4441 cgggatgttc agggcggaac ggtggcacca tcgtcctcct ctgaactgac ggggatcgcg
     4501 gttcagtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctttt
     4561 gccctgtaaa cgaaaaaacc acctggggag gtggtttgat cgaaggttaa gtcagttggg
     4621 gaactgctta accgtggtaa ctggctttcg cagagcacag caaccaaatc tgtccttcca
     4681 gtgtagccgg actttggcgc acacttcaag agcaaccgcg tgtttagcta aacaaatcct
     4741 ctgcgaactc ccagttacca atggctgctg ccagtggcgt tttgcgtgct ttcccgggtt
     4801 ggactcaagt gaacagttac cggataaggc gcagcagtcg ggctgaacgg ggagttcttg
     4861 cttacagccc agcttggagc gaacgaccta caccgagccg agataccagc tgtgtgagct
     4921 atgagaaagc gccacacttc ccgcaaggga gaacggcgga acaggtatcc ggtaaacggc
     4981 agggtcggaa caggagagcg caagagggag cgacctaccg gaaacggtgg ggatctttaa
     5041 gtcctgtcgg gtttcgcccg tactgtcaga ttcatggttg agcctcacgg ctcccacaga
     5101 tgcaccgaaa aagcgtctgt ttatgtgaac tctggcagga gggcggagcc tatggaaaaa
     5161 cgccaccggc gcggccctgc cgttttgcct cacatgttag tcccctgctt atccacggaa
     5221 tctgtgggta actttgtatg tgtccgcagc gcccgccgca gtctcacgcc cggagcgtag
     5281 cgaccgagtg agcgaggagg cgcaatttcc ccggcacctg caagacggtc accggacgcg
     5341 ctgatgtggg atattgtccc ctgattaagt ctttctattc cctgacgatc aattatcagg
     5401 ataggtgaac catgtcggtt tttcgctgaa cattttacgt taacctatct ctgttgcttt
     5461 tttctccttg cggagaaatg caagtcatcc ccgctggggc gtggctgact tcaggggaaa
     5521 agaccttgct tacgctggct ggacgccacc gacgcacggt cttttcccct ctttctggct
     5581 ctgtccggct ggacgccggc cacgccagaa attcacccgg cccccgtcag gctcacgcct
     5641 gaccacccac aaaaatccac ggtgctggac gcacctgacg aagcagcaaa gctgctcctg
     5701 attttgcggg tggtcagccg ctcgctatgg cgaactctga cgggggccgg aacaccggaa
     5761 aagaccgcac tggggtgccg ccttttcctc tcacacgctg gggcgtgctc gttgttcctc
     5821 cggggctgcg ccttttccgc atggggcgga aagaggctgg cctccgcggt gaaagctgaa
     5881 cgcaatcacc acggacgtga ccgccgccgc catgccttgg ggcatgtccg cggcttgtgc
     5941 agcgggggct gcgcatcacg ccggtaaacc cgtcgtctga aggagagaga ttgcatgttt
     6001 aatacggatt cgcttgcctg gcagtggcat atcccggagc tggcccggca gatttacccg
     6061 tgggtcagct ggctgtaccg ggagtattac agtctggccg gtttttttct ggttatcggc
     6121 ctgtttttcg gtctggctga actctgggcc ggccgaatgc gttttgtggg gctgagccat
     6181 aaggcgcggc gggagtatgc cgatcgctat aagcgcgata ataaccggac tgtgccattt
     6241 tcggtgctgg cctcgctgga ttcacccggg cggatggtca gggcacggcg acatgtctgg
     6301 aactacgcgt actggctgac cggcgctgcc ggtctggccc ttctgggggg gctggttgtc
     6361 gccgtgttta tcgagagctg gggagaaacc gtcctgcgcc cggacctcta tgcgaacgca
     6421 gattattacc tgctgcagcc ggctaacctg ttcgggatag cctggtgtgc cggagggctt
     6481 gtcgccggcg tccttctggc gcggctgctg gcgttcctgc tgcttgatgg tcattttgcg
     6541 gccgctgacg aggcggtgaa cgcgaaactg aatcaggaga gccggcgcag cagccagcgg
     6601 accggagaaa tgacagacgt ccgccacctg catttcgggg agccggtacc ggttaacgcg
     6661 ctggccgatt tttccacgga gcaggcccgg aaacagcagg cggtgtttct gggtaaggat
     6721 gagcagggcc agccggtgct ggtccctcgt gatacctggc gcaagaccaa tattcagatc
     6781 ctcggcctgc ccgggagcgg caagagcgcg atggccacca acgcgctcat tcgctgcgta
     6841 cgcgatttcg gggacgctgt ggtgtacttc gaccccaaag gggacgcgtg ggcgccgcac
     6901 gtcttccggg cgcactgccc ggacttcacc ctgctggatc tgcggccggg gaaaccggcg
     6961 cagctgaatc tgttccgcga tctcgaccag tacgccctga agaatctgct ggttgccggg
     7021 tttaacctga gtgagaccgg cgacgtggcg gaccactacc gcatcagcga gcagaaagcg
     7081 gcgaagctga ttgcggagca gttcccgcag ggggcaaaca ttcagcaggt gctggcggcg
     7141 gcgtacgcgt tgccggaggc cctgaaaaag gacgtgaagg ggctgatcac caagctggag
     7201 aacgtggccg acctgagcgt cctgcagacg gacagcggta tcgatgtggc ggggataatc
     7261 aacggcggcg gctgcctgta cgtcatcggc tcgatggacg atgaggcggt gatccgggtg
     7321 cagaagatgc tgtttgcccg ctgcgcgcaa atcatcattg cgcgggatga gttccggagg
     7381 tggccgcacg cgagcatcat gctcgatgag attaaatatc tgctgtcgaa gtacgtgctg
     7441 aacgcgctgg ggacgctgcg cagccgggac tgcaacctgc tgctcgcgca ccagtcgctg
     7501 ggcgacttcg gtcagtgcgg gcaggatctg cccgctgatt ttgtgaaaac cacggtactg
     7561 gataacacgc cgatccgctg gttctatcgc gcggccagcc aggagtccgc gcagtgggcg
     7621 gcgggccaga ccggggagat ccgggtggac gtggagcgcc gccgggccag ccgggaggcg
     7681 gggaacgtgg agcacatcag cggcgacagc ttcatccaga aggaggcgcg gccgctgttt
     7741 gacgtgaaca ccctgcaaca cctgcctgac gggttcgcgg tgatgaccgg gctgggcgtg
     7801 gcgcggctgg cgttcagcag cccgctgcgg gttgagcgcc gggaaatttc gctgaaaacc
     7861 ttcccggtgc tggcgaaaac ggacccgctg gcggagtacc tgcctccggc cgacggcggc
     7921 ggaccaaaag cggatgatga ttttgcgggg ctttactgat gacactggag cagtacaacg
     7981 tcagccacgc gaagcggcag gcgcggaacg cggagaaaac acggctgacc ctgcgctggc
     8041 tgcgggaaga gctgtgttcc acggcggagc tggttgcgcg acggctgggg attgcggcgg
     8101 tacagccggt ttaccgcttc ctggacagtc tggtggcgaa agggctgctg gtccgggcga
     8161 aatacccggt tgacggccgt caggtcagcg tgtgggggct gacgccgcac ggggtggcgt
     8221 tctcgttcga cgaggacgag ccgctgacgg acgttattcc gtttcagccg tcaagggtgt
     8281 cggcggcgca gctgccgcac cggctggccg tgcagtccct gcgcctctcc atggagggcc
     8341 ggggggccac cggctggcgg tacctgcacc gtatggccct gaaagggatg aaagtgccgg
     8401 acgcgctggc ggagctggac ggcagaacgg tggcatttga ggtagagcgc accgtcaaga
     8461 gccgcaggcg gtatcaggaa gtggtgtccg ggtatctgtt caaccgccgg gcgaacggca
     8521 tcgatgaaat ctggtacatc tgccctgacc ggccgacgct tgtccgggta cagcgggcga
     8581 ttctggcggt ggatgaaatc gtgaatccac agaccggcga ggcccgcaaa acggtggagc
     8641 tggaccggga gaggctgttc gcgtgcttta aatttatgac aacggagtga gggattatgg
     8701 cgctgtacat ggtatttatt gaggacgtgg aagacaaaga cggcgagcgc tgcattatgc
     8761 caaccagcga gcatctggaa gacatggtgg tgctcgcctg gcagaccggg gcgtatgttt
     8821 tctgttttgt cgaccgggcc cagcatctgg cgatgatgaa atccatcggg atgtatgacg
     8881 agcggctcaa cagcgacgaa aaagccacgt actttttcac cggtgaaaat ccgcaatggg
     8941 aacgggacaa acccacagga atgaagctgt gtgattacct ctatcgaaag gtggagcggt
     9001 tcgccatcga gaacggtgtg caggtggttg atgccgggaa gtgctcaccg gagcagatta
     9061 acgcgatgat tgaggtggtc agcgagcggc acggggaaag cgcggtgaac gagaagctga
     9121 aagcggagct gatgcgggaa tataacagcc gctattttaa cgtgccggtg cctgaactgc
     9181 cggaggacaa acccgcccgc cggtggtggc cgttcggcaa aaaataacgc cagcaccggc
     9241 atggccggtg ctgttattct tccccgagct tatcccgcat cacctgccag agtgtttccg
     9301 catcctcgtg aagccgttcg cacatgtcgg ccggggtatc catgtccagc tcgttcagct
     9361 tttccagcaa cagcagcgac tgatccttaa tgaatttagc catattgagg gcagtttttt
     9421 cctgcttcgt catgttctcc attcccttcg gtaagttctc agctaaacca gccggaccag
     9481 aagcgttttt tcttctgtgt cagttctgtt gtcaatctgt ccacttcggc ctggagtttc
     9541 cggcgctcat catcgtgctg gcggcgttct tcttcccggg atgttttatc ttccagcatc
     9601 agggtgacag cctgccggag atcggccagt tccttttgca ttgccagaag ctgttcctgt
     9661 gggaaatttt tttgtgtatt ttcacggtgt gaaatgccgg gaatttgttt ctcagggata
     9721 ttcacatttc cgtatacacg gatcagttct gacacatcca cgacagggtt gtttttcccg
     9781 tcacgtgaca cggtaacctt tccctgtttt acatgattgt atagcgttct tctggtaatg
     9841 cccgcggctt gtgctgcctg tgaaaggttg agcaatgttt tcgccactgt atacccctga
     9901 ttttatcggg tgtgaaaggt gtgtaatttc tcacggtaaa tgtatcgctg aattttaccc
     9961 gtttttccgt tcaggatgtg gttttctgac gcgctgcgct tgtcaaaccg gcgacggcca
    10021 cgcaatgaat tgcgcccccg tcatcggtta ttagaggctg tctgcccccg gatacccgga
    10081 ttctttctga tgcctgacgg tgacgatcac aatctggtcc atttcccggt cgtggcggta
    10141 gagcatcaca tagccactgt cgccaaaccc gatcaccagt tccttatatt ccagcggcag
    10201 aaacggcacc gggcggccaa tgtctggcaa tgttttcagt tgctggatag cccggacgat
    10261 cacctcacct ccttttctgg ctgccagccg atttttggtt ttgagaaagt cctgaaggcg
    10321 ttgtaaatcg cgctgtgcca gcgcggaaat cgttacctgt ggcatggcgg agcatcctgc
    10381 tcgttctcag ttccccaggt acttatccag gcttccgctt cttccgcagt aaggtgcaga
    10441 cccgtttcct gatagtgctg ccatgcggcc tgtccgtccc gcaggtactg gctgcgcttc
    10501 tcttcccggt cgatgtattc ggtgattgcc tccagcatta gcgcgtgcgc tgagcggtgc
    10561 cggtcatcgg ccagcgtttt cagccggtcc ttaagttctt catcgagcct gatggatgtt
    10621 gctgatgaca taaaagcccc tttgtagtca tatgtattac acatgactac tttaatccgt
    10681 ggcggcgcta tttgccagaa atacggcttt tgctgcccat ttttcagctt cgcttccact
    10741 aatcagggct gctacaggct cactcatcat ccaggactat tgcagcctgc gggttaaagg
    10801 acctgttact ggcggcaacc atcatcattg agcttttggg tggtgactgg tcgcggatcc
    10861 tgtaagccgt catttttcag actgaattca aaatttataa cctgtcgcat tgccgggtgc
    10921 cctgcgggat atagccagtg cctcattgcc gtcagcgttt cccgaaaaaa aaggtgacag
    10981 ccggtaagcg acacgttgtc cactcgtcca tcgtccagaa tatcaaacgt ggcggtcacc
    11041 tgaccttcga tgcgttttgc tgcggcctgt accgggtagg ttggctgcag tgtggttacg
    11101 ggttttagtg gtatcgcatg taccggagtg aggaacatgg ccgccagcac tgtcagagta
    11161 aaagctttca tggtctgaaa tccagagggg cgtttttgaa tatttgccag gtattttcat
    11221 ccggtgccgg cggaattttg gcgtgggtaa cggctgatat tgctgcttta cagagtttcg
    11281 gatctcctcc ttcagcaatg gcgctgttca gagtgccatc acgttgtatg gatatccgca
    11341 cggtgcacat ttttcccgca taggcctctg gttcaaacat ctgcatttca acagcggtct
    11401 tgatggccgc agcgtagcta ctgattttag atgacttatc cagagtataa attttcggcg
    11461 gctgggtggt ggaatgctta caggccgtca ggagtaaggg gctgcacaat gccagcagga
    11521 taaagggtat taaacgcatc gggttgatga cctgtcagta aaaaatgtta catgtatatt
    11581 tgtcactctt tacggccacg ttcatttaca taaacacggc ttatcgtatc gtttttacac
    11641 caaacggccc gccatgcagt tgcgtgcata ggcatgcagc gggttacaat ttaccgggcg
    11701 cgtttccggc ggttttccgg gtggtttgtt gcctgtttta tcccgtagcc gccggaaacg
    11761 ccctcagacc gtctgagcgg tgcgggcaat gcgacgttat ggtaaatacc ccttaccggc
    11821 ggcgctgtgg ctgccaggtg aaggtggttt ccgtagcctc gtatttctcc cgacgcattt
    11881 cctgcgcccg cctggcttca cgttcgtcct gaatggcacg aagcaccctt tcccgcacct
    11941 gttgttttac cgcctttggc agcgttttgt tc
//
