LOCUS       CP018357                3851 bp    DNA     circular BCT 25-JAN-2017
DEFINITION  Klebsiella oxytoca strain CAV1752 plasmid pCAV1752-3851, complete
            sequence.
ACCESSION   CP018357
VERSION     CP018357.1
DBLINK      BioProject: PRJNA246471
            BioSample: SAMN03733848
KEYWORDS    .
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Mathers,A.J., Stoesser,N., Chai,W., Carroll,J., Barry,K.,
            Cherunvanky,A., Sebra,R., Kasarskis,A., Peto,T.E., Walker,A.S.,
            Sifri,C.D., Crook,D.W. and Sheppard,A.E.
  TITLE     Chromosomal Integration of the Klebsiella pneumoniae carbapenemase
            gene (blaKPC) in Klebsiella Species: Elusive but not Rare
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Mathers,A.J., Stoesser,N., Chai,W., Carroll,J., Barry,K.,
            Cherunvanky,A., Sebra,R., Kasarskis,A., Peto,T.E., Walker,A.S.,
            Sifri,C.D., Crook,D.W. and Sheppard,A.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2016) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: 110x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/05/2016 17:46:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 4.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,264
            CDS (total)                       :: 6,144
            Genes (coding)                    :: 5,948
            CDS (coding)                      :: 5,948
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 78 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 68 of 196
            Pseudo Genes (multiple problems)  :: 37 of 196
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="CAV1752"
                     /isolation_source="Perirectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:571"
                     /plasmid="pCAV1752-3851"
                     /geo_loc_name="USA:Virginia"
                     /lat_lon="38.14 N 78.45 W"
                     /collection_date="2012-12"
                     /collected_by="Clinical Microbiology University of Virginia
                     Health System"
     gene            join(3617..3851,1..1292)
                     /locus_tag="AGH21_00005"
     CDS             join(3617..3851,1..1292)
                     /locus_tag="AGH21_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="APM29113.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQREGASVLQGKPEEIRELID
                     ASPYVKKYTSGVLSFAEADLPPGQREKLMASFERVLMPGLDKDQYSILWVEHADKGRLE
                     LNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTVVNGRLGLHDPNDPENRRALVTPSGL
                     PETKQEAAETITRGLLMLASSGELKTRDDVTGALTAAGFEVVRTTKNSISIADPDGGRN
                     IRLRGAIYEQSFNGLEGLRGEIESTAAAYRREAESRIQQARERCQIGTERKREENQRRH
                     QRPRTGDERGDAVEPAERAANGRADVADHHHGVRAAVRQPERGAVVPGEPDSGEPGGNQ
                     AAERYAVETADADVGRDLSGKPQRTVSGAAGGDESRNGPEGGQQDAERGEAGTGVEQND
                     GTGKTIAERVRAATAGLLEKAGRVGERLRGMAEDVWSYATGERGAERARVGLEQAGAEF
                     ERAAAPVIDRLNEIEQQRYGQTTEKEPEKTADLVPERQRTLSHGDREYGD"
                     /name="nuclease"
     gene            complement(1598..1825)
                     /locus_tag="AGH21_00010"
     CDS             complement(1598..1825)
                     /locus_tag="AGH21_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="APM29114.1"
                     /translation="MTKVIYSALAAIIALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DAGLIGYTDANGVKQQINKQEVKEVSEIPN"
                     /name="hypothetical protein"
     gene            complement(1839..2075)
                     /locus_tag="AGH21_00015"
                     /pseudo=""
     CDS             complement(1839..2075)
                     /locus_tag="AGH21_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013512138.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(2530..2664)
                     /locus_tag="AGH21_00020"
     CDS             complement(2530..2664)
                     /locus_tag="AGH21_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="APM29115.1"
                     /translation="MHEPPVQSDRCALSGNYHLDSNPDGHEKTPLAAATGNKGVQDGI"
                     /name="replication initiation protein"
     gene            complement(2689..2943)
                     /locus_tag="AGH21_00025"
     CDS             complement(2689..2943)
                     /locus_tag="AGH21_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="APM29116.1"
                     /translation="MKSGRAGERMRRCRFSIGSAPLTSITKSDAQISGGETRQDYKDPR
                     RFPLVAPSCALLFLPFGLPVSFRCYGRVCLIPRLTLSSG"
                     /name="hypothetical protein"
     gene            2870..3077
                     /locus_tag="AGH21_00030"
                     /pseudo=""
     CDS             2870..3077
                     /locus_tag="AGH21_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            3286..3627
                     /locus_tag="AGH21_00035"
     CDS             3286..3627
                     /locus_tag="AGH21_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="APM29117.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="mobilization protein"
     Typing          3019..3203
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.701"
                     /coverage="86.0"
                     /evalue="5.789999999999997e-54"
     Typing          complement(2555..3036)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.741"
                     /coverage="92.0"
                     /evalue="0.0"
     Typing          4..1289
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.301"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3308..3621
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.78e-58"
     Typing          3618..3850
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.436"
                     /coverage="89.0"
                     /evalue="5.6299999999999986e-42"
ORIGIN
        1 gggttctgat gcccggactc gataaagatc agtacagcat tctgtgggtg gaacacgcag
       61 acaagggtcg tcttgagctg aattttctga tccccaatac cgaattactg acggggaaac
      121 gcctccagcc gtattacgat cgtgccgacc ggccgcgcat cgatgcgtgg cagacagtgg
      181 tcaacggcag gctggggcta catgatccga acgacccgga gaaccggcgg gcgctggtca
      241 cgccgtcagg actgccagaa acgaagcagg aagccgcaga gacgattaca cggggtttgc
      301 tgatgttagc ctcatcagga gagctgaaaa cgcgtgatga cgtcacaggg gcgctcacag
      361 cggccggttt tgaggtggtc agaaccacaa aaaacagtat cagcattgcc gacccggacg
      421 gggggcgaaa tatccgactg aggggagcca tttatgagca gtcttttaac ggcctcgagg
      481 gacttcgagg agaaatcgaa agcacagcag cagcttaccg aagagaggct gaaagccgca
      541 ttcagcaagc acgagaacgt tgtcaaatcg gaactgaacg caagcgcgaa gagaatcaac
      601 gacgccatca gcgcccacga acaggggatg aacgcggcga tgcagtcgaa ccggctgagc
      661 gtgctgcgaa tggtcgggcg gacgtggctg accatcacca tggtgtcagg gctgctgttc
      721 gccagcctga gcggggtgct gtggtaccag gggaaccgga tagcggcgaa cctggcggaa
      781 atcaggcagc agagagatac gctgtcgaaa ctgcagatgc agacgtgggg cgtgacctat
      841 ctggaaaacc gcaacggacg gtttctggtg ctgccggagg ggatgaaagc cgaaacgggc
      901 cggaaggtgg acaacaagac gcggaacgcg gtgaagctgg tacgggagta gagcagaatg
      961 acggaactgg aaaaacaatt gctgagcgcg ttagagcagc tacagcagga ctactcgaga
     1021 aggctggacg agtgggagag cgccttcgag gaatggcgga agatgtctgg tcttatgcaa
     1081 cgggagaacg cggcgctgag cgagcgcgtg tcgggcttga gcaggcaggt gcagagtttg
     1141 agcgagcagc tgcgccggtt atcgacaggc tgaatgagat tgagcaacag cgatacgggc
     1201 aaacaacgga aaaggaacca gagaagacag cagatctggt accggaacgg caacgcacat
     1261 tatctcatgg tgacagggag tatggagact gataaacgcc cgtctcaggt ggcctgagcg
     1321 tgacggggat catggccatt ttctgcgatt tcagcacaca gagagtcttt tttaaccgaa
     1381 atcaccggct atgcagtgat gtgaataagt gatgcatatg cgtaaaataa atcaggtgcg
     1441 tttctggtgg ttttcggtgt acttgccacc accttttgcc cgtcagccat cagaaacgcc
     1501 ctgagtgcct ccggcgcggt gcgagtaagg gatgttatgt ttaattggac ggttactagc
     1561 atgtaaaaaa aaaccgccac taggcggttt tttatgatca gttaggaatc tctgagactt
     1621 ctttgacttc ctgtttatta atttgctgct tcacgccgtt agcatcggta tagccaatca
     1681 acccagcatc cgattctttg ggtttgccat cgctcacaat ggtacggcca tcattcgtat
     1741 gaattgcata gcttgtcctt gtgcatcctg tcagagctga aagtgcaatg atcgcagcta
     1801 gcgctgaata aattactttc gtcatagaac gttccttatc agttatctag ttcactcatg
     1861 ttggtaattt tgtcgcggtt gatatgttct gtttttcctg tctgtgcatc cttgtaagaa
     1921 accagcccgg tgtcactatc aatctgaggc ttaccgtctg cgacgatagt ccgtccgtcg
     1981 gtagttttaa tggcctgatt ttaggaggtg ccggttactg agaaggttaa aactgaagtt
     2041 attaagagaa accaatgctt ttttagatat cccatatccc tagtactggc gaagttagta
     2101 tctaacttac taataatagg cattgttttg gcttctgcat cggaagggga gataaaatgt
     2161 aaaaaaagaa atcttatgtt aaatcaggtg gcagaatgct gtgttaaacg gctagttgct
     2221 aatcataatc attttgtaac gcagggggat ttcttccgcc gataaatgca ttccagtgat
     2281 ggattcatta caaggaacat cataaatccc atggatatcg atcctgcgaa gaaaggatct
     2341 tcttgagatc atttttttct gcgcgtaatc tcttgctctg aaaacgaaaa aaccaccttc
     2401 cggtggcttt ttcgatggat agaggatcct ggcagattct ctaaccgagg taactgggtg
     2461 tacaagaccg caattaccaa aactgttctt tcagtgtagc cgcagtcagg catccacttc
     2521 aagacctctt taaataccat cttgtacacc cttgttacca gtggctgctg ccagtggcgt
     2581 tttttcgtgt ccgtccgggt tggaatcaag atgatagtta ccggataagg cgcagcggtc
     2641 ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct acccggaact
     2701 gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc
     2761 gaaaggcagg aacaggagag cgcacgaggg agctaccagg gggaaacgcc tgggatcttt
     2821 atagtcctgt cgggtttcgc cgccactgat ttgagcgtca gattttgtga tgcttgtcag
     2881 gggggcggag cctatggaaa aacggcaacg ccgcatcctt tctccagccc ttcctgattt
     2941 cagcgcagtc tctctatctt gtcattcgtg agcaggtaac gcccgccgcc gtctcacggc
     3001 aggagcgcag cgaccgagtg agcgaggagg cgtcatgtca tcaccacctg cattttgcac
     3061 ttacgccccg cagctgaatt tttcccctgc cacatgaagc actttcccga attaccactt
     3121 cgatttatca tattaagcca gtatacactc cgctagcgct acgtgactgg ttcagggctg
     3181 cgccccgaaa cccgctaaaa accactgacg cgcctgcggc ttgtccaacc ccgcgccgac
     3241 cgggaaaggc tttcccgtcc gccacggcgt tatcaggagg cgattctggc agataaacgc
     3301 aacaagatgc tcacgatgtg ggtgacggaa gacgaacatc ggcggttgct tgagcggtgc
     3361 gacggaaaac aactggcggc gtggatgcgc cagacctgcc ttgatgagaa accgacacgc
     3421 gccggcaggc ttccctcgat ttcgccggcg ctgctgcgtc agcttgccgg catgggcaat
     3481 aacctgaacc agatcgcccg gaaagtgaac gccggtggtg ccggtcatga ccgggtgcag
     3541 atcgtggcag cgctgatggc gatcgatgcc ggtctggaac ggttgcggca tgccgtactg
     3601 gaaaaagggc cggatgatga ttgttaaatt ccatccacgg ggtcgcggcg ggggcgccgg
     3661 tccggtggac tatcttctgg gtaaagaccg gcagcgtgaa ggtgccagcg tcctgcaggg
     3721 gaagccggaa gaaatccggg agctgattga tgcgtcgccg tatgtgaaaa aatatacctc
     3781 cggggtgctg tcattcgcgg aggcggatct gccgccgggt cagcgtgaaa aactgatggc
     3841 cagctttgag c
//
