LOCUS       CP016042                3205 bp    DNA     circular BCT 01-SEP-2020
DEFINITION  Escherichia coli isolate Co6114 plasmid pC06114_8, complete
            sequence.
ACCESSION   CP016042
VERSION     CP016042.1
DBLINK      BioProject: PRJNA321990
            BioSample: SAMN04963292
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Both,A., Huang,J., Kaase,M., Hezel,J., Wertheimer,D., Fenner,I.,
            Gunther,T., Grundhoff,A., Buttner,H., Aepfelbacher,M., Rohde,H. and
            Hentschke,M.
  TITLE     First report of Escherichia coli co-producing NDM-1 and OXA-232
  JOURNAL   Diagn. Microbiol. Infect. Dis. 86 (4), 437-438 (2016)
   PUBMED   27681362
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Both,A., Huang,J., Kaase,M., Hezel,J., Wertheimer,D., Guenther,T.,
            Buettner,H., Aepfelbacher,M., Rohde,H. and Hentschke,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2016) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-APR-2016
            Assembly Method                   :: SPAdes v. 3.8.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 192.0x
            Sequencing Technology             :: Illumina MiSeq; Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/14/2016 13:43:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 3.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,155
            CDS (total)                       :: 5,053
            Genes (coding)                    :: 4,954
            CDS (coding)                      :: 4,954
            Genes (RNA)                       :: 102
            rRNAs                             :: 8, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 34 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 8 of 99
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Source bacteria available from Moritz Hentschke, MVZ Labor Dr.
            Fenner und Kollegen, Bergstrasse 14, 20095 Hamburg, Germany.
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="Co6114"
                     /db_xref="taxon:562"
                     /plasmid="pC06114_8"
                     /geo_loc_name="Germany: Hamburg"
                     /collection_date="2015-05-01"
     gene            424..672
                     /locus_tag="A8V37_25765"
     CDS             424..672
                     /locus_tag="A8V37_25765"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_000800279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ANM85928.1"
                     /translation="MKNVRMQFDLPEDRLDELDSLMKKCGISTKKELFNYALTMLEWAV
                     DESESGHEIAAIDRDSKQFYALRMPILKRVNRTSTVN"
                     /name="hypothetical protein"
     gene            746..1117
                     /locus_tag="A8V37_25770"
     CDS             746..1117
                     /locus_tag="A8V37_25770"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ANM85926.1"
                     /translation="MSDSLDGFTEGDTQKEKQKVKQPKPFFNDVEESADRRHLRWTKSG
                     VTLAMTILVAVMNWMVISMLYHVLDEELSLIRSGFMRADERTVTTSVYLALIAGTVAEV
                     SALFLIIVKSVFKNNNNDI"
                     /name="hypothetical protein"
     gene            complement(2144..2338)
                     /locus_tag="A8V37_25775"
     CDS             complement(2144..2338)
                     /locus_tag="A8V37_25775"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase"
                     /protein_id="ANM85927.1"
                     /translation="MNKQQQTALNMARFIKSQSLALLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFEAESETGT"
                     /name="RNA polymerase"
     Typing          1977..2061
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          2376..2473
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.98"
                     /coverage="99.0"
                     /evalue="4.84e-41"
     Typing          2598..2853
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.124"
                     /coverage="89.0"
                     /evalue="5.65e-115"
     Typing          complement(1408..1951)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ggaggtgttt aggtgtaata tacaatctgg aacccatcac ggtgggtggt gggtaaactg
       61 gagattttat ctgtgagcaa gttaactcaa acctgctcgt cgacctccgg gaaaagtgag
      121 gactgtaccg actttgaaga actgcctcct aaggagcttt ctttgaagga ttggttttca
      181 gtctttactg aggtcgatga gtaataacca ccatggagga gtattcacaa cctcctccat
      241 ggtggggata acctgcttgc ggaaaaagag ataaggttaa gtcttatcta aattaggagc
      301 atcataaaaa tatgaaactt aaattattta taagtagata tatataaata atacaggccc
      361 taatagtgaa gaatgtgcag catagagtgt aaggatgaca tgctggcccc tcggagatta
      421 gttatgaaaa atgtacgtat gcaattcgat ctgcctgaag ataggttgga cgaactcgat
      481 tctttaatga agaagtgcgg cataagtact aaaaaagagt tgttcaacta cgcgttaaca
      541 atgcttgagt gggctgtgga cgagtccgaa agtggtcacg aaatcgcagc tatcgataga
      601 gacagtaagc aattttatgc tctacgaatg cctattctta agcgagtgaa ccgtacatct
      661 actgtaaatt aacttatcac ttgatgtgtt aaggagaagg ccagtttaag ctggccttct
      721 tttttttatt caagaggatt tgattgtgtc agacagcctg gatgggttta ctgagggcga
      781 tacccaaaaa gaaaaacaaa aggtaaagca gcccaaaccg ttctttaatg acgtcgagga
      841 gtctgctgat agaagacatc ttagatggac taaaagtggt gttactttag ctatgactat
      901 tttggtcgca gtaatgaact ggatggtcat aagtatgtta tatcatgttc ttgatgaaga
      961 actttcgctc ataagaagtg gctttatgcg ggcggatgaa aggacggtaa ctactagtgt
     1021 ttacttggct ttgattgcag gaacagtagc agaggtgtca gctttattct tgattattgt
     1081 aaagtcagtt tttaagaata ataacaatga tatatagatt taacttcggc cacctctggt
     1141 gaagttacgc atgtatttgg tcaatctatc ttgagatcac tctaatcaaa atgtaatctc
     1201 ttgaggttga aacgaaaaaa ccgccttggc gggcggtttt ttcgaaggtt ctaagagttg
     1261 gcgctctttg aaccaaggtc actggcttgg aggagcacag tcaccaaatc tgttctttca
     1321 gtttagcctt aacaggcgca tgacttcaag acaaactcct ctaaatcagt taccagtggc
     1381 tgctgcctgt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1441 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1501 gacctacacc gaactgagat accaacagcg tgagctatga gaaagcgcca cgcttcccga
     1561 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1621 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1681 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1741 cggcgcggcc tttcgttggc ctttgccttt ttgctcacat gttctttccg gccttatccc
     1801 ctgattctgt ggataactgt attaccgcct ttgagtgtgc cggcaccgct cgccgcagcc
     1861 gaacgactga gcgtagcgag tcagtgagcg aggaagcgga agagtgtcgg aagttgcatt
     1921 ttctccttac gcatctgtgc ggcatttcac atcggctatg gtgcactttt catacaatcc
     1981 gcactgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     2041 ggctgcgccc cgacacccgc taaaaccccc tgacgcgccc tgacgggctt gtctgctgcc
     2101 gaaaacgctc gcggacaagc cgtgagcgcc tccgtcagct tcgttatgtt cctgtttcac
     2161 tttcggcctc aaagcgaacc tggatgctgt tctggagttc ttccgcgagt tcgtgcagtc
     2221 gttcgcacat ggccgcctgt tcgtcggcat ccagagcatc cagtttttca agcagtgcca
     2281 ggctctggct tttgataaat cgcgccatat tcagcgcagt ttgttgctgt ttgttcacct
     2341 tccgttgttc tccgtcagta ccgggtgtcg ggtagaccct gacccggtgg taattgtaag
     2401 acgttgcggg cgacggtatt acaattgcac atcctgtcct gtttctttag gacgtatcat
     2461 ggcacagcat atcaactgat aaataatccc tgtgcagtgc agccgctgtt gcataaaatt
     2521 tcgctctgcg tcgagtgttt cttaatcaat aattccgctg atactccgga aaaacgcgag
     2581 tctgttctca tgcctgaaat gcccacaccc cacgcaaaaa caattttttg ctgatttttc
     2641 tttataaata gagagttatg acaaattagt tcttcttgct ctctttgtga tatttaaaaa
     2701 agcggtgtcg gcgcggtgtt gtagctgcgc caacaccgct ttttaggggt ggtactgact
     2761 attttcataa aaaacatcat cttatattag gggtgctgct agcggcgcgg tgtgtttttt
     2821 tacaggacgc ccctgggggc gctgctaggg gtgtctgttc agatatgtag caatgagtca
     2881 gggattcacg aactgaaaat gtccgaaaat gtgtctcatg ggcactgtat gcgcccaaac
     2941 cgatcgccat tcactttcat gcatagctat gcagtgagcc gaaagcgatc ctgacgcatt
     3001 tttccggttt tcactgggtg aaacatctct ttttgcggtg tttgcggcag aatcgcgttc
     3061 agcgcgtttt agcggtgcgc gtaatgcgac gttatggtaa atgaggttga gatttggctg
     3121 attgtctcat cctgttacaa ctggtgttgt atttttgacc gctatcgttc tatactcgtt
     3181 ttgtgctcct gttacactcg acaaa
//
