LOCUS       CP016041                4071 bp    DNA     circular BCT 01-SEP-2020
DEFINITION  Escherichia coli isolate Co6114 plasmid pC06114_7, complete
            sequence.
ACCESSION   CP016041
VERSION     CP016041.1
DBLINK      BioProject: PRJNA321990
            BioSample: SAMN04963292
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Both,A., Huang,J., Kaase,M., Hezel,J., Wertheimer,D., Fenner,I.,
            Gunther,T., Grundhoff,A., Buttner,H., Aepfelbacher,M., Rohde,H. and
            Hentschke,M.
  TITLE     First report of Escherichia coli co-producing NDM-1 and OXA-232
  JOURNAL   Diagn. Microbiol. Infect. Dis. 86 (4), 437-438 (2016)
   PUBMED   27681362
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Both,A., Huang,J., Kaase,M., Hezel,J., Wertheimer,D., Guenther,T.,
            Buettner,H., Aepfelbacher,M., Rohde,H. and Hentschke,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2016) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-APR-2016
            Assembly Method                   :: SPAdes v. 3.8.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 192.0x
            Sequencing Technology             :: Illumina MiSeq; Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/14/2016 13:43:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 3.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,155
            CDS (total)                       :: 5,053
            Genes (coding)                    :: 4,954
            CDS (coding)                      :: 4,954
            Genes (RNA)                       :: 102
            rRNAs                             :: 8, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 34 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 8 of 99
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Source bacteria available from Moritz Hentschke, MVZ Labor Dr.
            Fenner und Kollegen, Bergstrasse 14, 20095 Hamburg, Germany.
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="Co6114"
                     /db_xref="taxon:562"
                     /plasmid="pC06114_7"
                     /geo_loc_name="Germany: Hamburg"
                     /collection_date="2015-05-01"
     gene            join(1974..4071,1..50)
                     /locus_tag="A8V37_25750"
     CDS             join(1974..4071,1..50)
                     /locus_tag="A8V37_25750"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_001557504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="ANM85925.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRENRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="conjugal transfer protein"
     gene            complement(307..1008)
                     /locus_tag="A8V37_25755"
     CDS             complement(307..1008)
                     /locus_tag="A8V37_25755"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_001281396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="initiator RepB protein"
                     /protein_id="ANM85923.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="initiator RepB protein"
     gene            complement(1541..1795)
                     /locus_tag="A8V37_25760"
     CDS             complement(1541..1795)
                     /locus_tag="A8V37_25760"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="ANM85924.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobilization protein"
     Typing          complement(1797..1973)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1974..2332
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(308..1008)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.858"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1888..4070
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.038"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 cacgaacaca caccaccgag aggagacatg atcgcggttg gtcacagtga atgtgaacaa
       61 tattttgttt tgtttaacat cgtgttatgt tcgtcgatgt tcttcgatgt tcatcgacag
      121 ggatgaatgc aagaagcaca ctcacataac gcaagcatag ttttgttttt ccgcccccaa
      181 aaattggggg tcttttttcg ctgcatcaga gcgtttttga cgcaattttt cacttcaata
      241 gtaccttcgc actactaaca caaaaaaaac gcagcaggga gcgttttagg tgccttaaaa
      301 tcgattttag atcgtcatct tgatctgttt gtcttccttg aagcgaaaat gcagggctac
      361 aaccgtgcgc ccctttttga tcgtctcaac agttacaacc agatcgcttt tttcgttcag
      421 ttcggcgatg gctggcttga ttatcatctt attcaagtct ttgaattgtt gatatttatc
      481 ttcaacggca agcatggttc ggaaatcatc gatagctatc gttctgtcac cggtgctgcg
      541 gaactgctgg agcagctcgt aaatgcggat gctgtaggtg ctggaaaggc tggagacgtt
      601 cttcacgact acccgcgtaa actgcccctt gagctgtgtc agatacggca tgatggcatc
      661 ggagaaggtg atatctactt tcgcctcacc tttgaaatac tgcgcccggt actgcaccca
      721 gcggaactct tctcgttttt cctcgttctt gatgattact gacctgtccc agaggcgatc
      781 tatcgcttcc ttgagcctag cttcagcgtg ctgccgtccc atgtcaggaa aggcatcatg
      841 aaactctgca gccgtgatgg tcatggtttt ttgttcatcc ggcgaagggg aatcttctcc
      901 ggatttcagg cggctgatac agaccagcag gaagcgctgt tcctgcaaag tgagcttgta
      961 gcttgcctcg ataagggcgt ttgatttgta agctatcaga tctgacattt cgcttgctcg
     1021 ttgctattgg tggcgttttt ttactgtacg ccacttatca tatgggtgga agtttttttt
     1081 ttgcaacccc ctttttctgt ggattacacc cccaaaagct ccacctttca cccccaaaag
     1141 ctccaccttt cacccccaaa agctccacct ttcaccccca aaagctccac ttatcttgcc
     1201 agaacatctt taaaatcatg gtgttacaag acctaaaaag gttttaaaaa aaagatctaa
     1261 aaataagatc ttaaaaactt aacggcgagc ctgtggataa cttggggtgc tgaaaacaaa
     1321 aaaccaataa aaacaatgcg ttgcaataaa gcagaattgc aagtttcgaa tacaccccct
     1381 ccaaccctgt ggaaacccag tattcatgcg ggttcagcgc atatcacctg ccgaaaaacc
     1441 gtgcgtatgt gctcaacctg gcgactgatt cacttccgct tcctcgccca ctcgctcgct
     1501 aacgctcggt cgctggggct gcggcgagcg gacgatacaa tcactgttct ggcagcgcct
     1561 ccagctcgtt aatcagcggc agtatgcgcg tcacatcagc ctgtcgtgtc acctctgcct
     1621 tcaggaactg gagtaaccag gcacgcaact gcacctcctc cggctttctg acctttgcca
     1681 gcaatgcagc acccacaaca accttctgcg ctgtgtccag gctccggctc ttcgccttca
     1741 ggcgctgtaa tctcgcctca gcatcagcaa tcttctgttc gagcgttctg ctcatgatgt
     1801 aactccgtgc gcgatgaaaa atcgcatttt agcgcgtcac tggtagttta aaaactgagc
     1861 tggcataatg cacggcaaat cacgaagtgc gcacttatac aatctccact ccgtttcgat
     1921 tgtgtgcgcc ctgcggggct gaaagaaaac tacaaaaagg cattacggca gaaatggcga
     1981 tttatcatct cagcatgaaa attatctcca gaagcagcgg ctacagcgcc gtagcttccg
     2041 ctgcctatcg ttccggttca ctgatgcttg atgagcgtac cggactgact cacgactaca
     2101 cccgcaaaag cggtgtggct gaggctgtga ttctgacccc cgccaccgcc cctgcctggt
     2161 gtacgaaccg tgccgaactc tggaatgccg ttgagaaggc cgaacgccgg aagaactcac
     2221 aacttgcgcg ggaaattgaa ctcgccatcc cccacgaact gccgcaggat gccgcacggg
     2281 aaaccgttct cgcctttgtc agagaaaact ttgtcagtca gggcatgatt gctgatgtgg
     2341 cgttccatca catggataaa acaaatcccc atgcgcacat catgctgacc acgagggctg
     2401 tcggacctgc tgggttcagc ggaaaggtca gggactggaa cgaccggaca catgccgaga
     2461 cgtggcgggc atcatgggct gaccatgcaa acagggcact ggcgaacgcc ggttatcagg
     2521 aagagataga ccatcgttca tacgagcgac aggggcttga gaaaacgcct ggtattcacc
     2581 tcggaaagtc tgcctgtgcg atggaaacac gcggtatcga aacagaacgc ggcgaacaga
     2641 atcgtctgat taacaggctt aacctggaaa tacagatttc ccgcaccgaa ctcaggaacg
     2701 gagggctgac agagcctgcc cgccgtgtgg ctgaccttct gaatatcggg cttcctgaca
     2761 acgcaacttc gtacacacta cgtgacatta tcgaagcgtt gccgaaggac agtaactcgg
     2821 catggaaact caccagtaat gccatgtcga tgatggcaga tatgcaggcg acgcgcagac
     2881 gctgggaaga gctgaacacc gaacgaaagg aagcgatgag ccatgctgcc agcctgaaaa
     2941 aatcatcgcc gttttcgtcc ggtttttccc gtattcccct gatgcaatgg gcggcaccgg
     3001 aataccgtag tgagcaggga aaaatccagt ctctgagcca gcaaatggaa aaattacgac
     3061 agcattaccg gaaggtggag aaacaggaca ttcccgcgag tcagatggca tttgaaaaac
     3121 agtggcagca gtggggggaa tccggccttg cagctctcag agcccagttg cttaaacgtg
     3181 aagaggaatt gcgccggaag gaacaggaag atactgaacg tagtcgcgcg gagcagctca
     3241 ggaaaaatga caatgccgtt atggattcag gggtgctgag agcagtcgtt acggggtatg
     3301 gcgaggctcc catgcctggt aacggggaaa tgacctgtta ccttctgctt cacaaccgga
     3361 acggagaata tacgctctgg gggaacgagc tggagaaata ccgcaccaga atctttgaga
     3421 gtgttgattt aatgcgtgac aggagtggtt atatctgtga ccgaagcgaa atcggccaac
     3481 gtccgccact gcaacgtgtt tattcctctg caacctttga gcaactgctg acgcaggttt
     3541 gccagacatg gccgcaacat acccgcaatc tccgccagcc gaagacatgg ccggagtctt
     3601 tctgtcttgg agaagaccgc cagccagcca tgccatcacc agcggcaagg aaggtggatt
     3661 ttatgcaggg acgtctgcca ccgacgctga tgccggtcat gagttctgtc gaccgggaaa
     3721 accgtcagct tcagcttttg ctggtcatgg gcgtggatga ctctctgggc ggtgttatcc
     3781 ggcttaatgg tacgctgtat cccgcgtttg ccgttccctc agccgataac aggcatctgg
     3841 tcatcagtgc gctgaccgat aaggggttac ggtatgcagg gtatggtgtt gccgtaaatc
     3901 acgatgcaga cagccacata tcacctgcac cagaactgat gcagtttcat ctgaaaaccc
     3961 gtgaggcacc gctttttgct gccgtcaaca caccagaaaa acagccggat catctgttcc
     4021 gctcgctggg ttttaatcgt acctgggagg aatggcagcg cgaggaagat g
//
