LOCUS       CP016040                4634 bp    DNA     circular BCT 01-SEP-2020
DEFINITION  Escherichia coli isolate Co6114 plasmid pC06114_6, complete
            sequence.
ACCESSION   CP016040
VERSION     CP016040.1
DBLINK      BioProject: PRJNA321990
            BioSample: SAMN04963292
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Both,A., Huang,J., Kaase,M., Hezel,J., Wertheimer,D., Fenner,I.,
            Gunther,T., Grundhoff,A., Buttner,H., Aepfelbacher,M., Rohde,H. and
            Hentschke,M.
  TITLE     First report of Escherichia coli co-producing NDM-1 and OXA-232
  JOURNAL   Diagn. Microbiol. Infect. Dis. 86 (4), 437-438 (2016)
   PUBMED   27681362
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Both,A., Huang,J., Kaase,M., Hezel,J., Wertheimer,D., Guenther,T.,
            Buettner,H., Aepfelbacher,M., Rohde,H. and Hentschke,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2016) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-APR-2016
            Assembly Method                   :: SPAdes v. 3.8.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 192.0x
            Sequencing Technology             :: Illumina MiSeq; Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/14/2016 13:43:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 3.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,155
            CDS (total)                       :: 5,053
            Genes (coding)                    :: 4,954
            CDS (coding)                      :: 4,954
            Genes (RNA)                       :: 102
            rRNAs                             :: 8, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 34 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 8 of 99
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Source bacteria available from Moritz Hentschke, MVZ Labor Dr.
            Fenner und Kollegen, Bergstrasse 14, 20095 Hamburg, Germany.
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="Co6114"
                     /db_xref="taxon:562"
                     /plasmid="pC06114_6"
                     /geo_loc_name="Germany: Hamburg"
                     /collection_date="2015-05-01"
     gene            join(4118..4634,1..44)
                     /locus_tag="A8V37_25725"
     CDS             join(4118..4634,1..44)
                     /locus_tag="A8V37_25725"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="ANM85920.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKQQLDTLSTTVSTQLSTTEAELSRQQKNLLWQVIKGRVLFPALTA
                     LSVTGGIFLGCWGLIQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="mobilization protein"
     gene            51..284
                     /locus_tag="A8V37_25730"
     CDS             51..284
                     /locus_tag="A8V37_25730"
                     /inference="EXISTENCE: similar to AA
                     sequence:SwissProt:P13660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="ANM85918.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHAVTQQNNA
                     ILNERVVNLSQQVQLLAGQVDRLSRLFITNNR"
                     /name="mobilization protein"
     gene            complement(1013..1852)
                     /locus_tag="A8V37_25735"
     CDS             complement(1013..1852)
                     /locus_tag="A8V37_25735"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ANM85921.1"
                     /translation="MLTLFIFFVLLIAACFFCFAPPRRGYDRNEIIPYKIKLSINKYRL
                     YIYSSGKVRQYLLFLVILSLYYSIAEPFKSELIKNISYSLMAAFIFDTGLNFSKENITK
                     GVISTRWHNDLYSSFERMKAINKIYYPSNKEINTEGLSKAITSSLFNDDANSFAKRDFR
                     LMWDLSSEKYLSYKEIIIRKGDKLDAVCLRFINDDYKFLVNFNRDEEVFKYFPSIMQPS
                     LKTYRALSRLVNSIKDPSRFKFTTESLEMELLEYLELRNELFNDIEEVMGSYAQRAP"
                     /name="hypothetical protein"
     gene            complement(2863..3054)
                     /locus_tag="A8V37_25740"
     CDS             complement(2863..3054)
                     /locus_tag="A8V37_25740"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ANM85919.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="hypothetical protein"
     gene            3117..3440
                     /locus_tag="A8V37_25745"
     CDS             3117..3440
                     /locus_tag="A8V37_25745"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="ANM85922.1"
                     /translation="MLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQAVREQGGR
                     DDS"
                     /name="mobilization protein"
     Typing          2691..2775
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          complement(2122..2680)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.939"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3431..4632
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.751"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 ggacggtggg cgacaagaac gccgtgaggc tggtcaggga gtaaacgagt atgacggaac
       61 tggaagggca tttgctgaac gccttagagc atttgcaaca ggactatatg cggcggctga
      121 acgagtggga gagcgccttc gcagaattgc agaagatgca cgcggttacg caacagaaca
      181 acgcgatact gaacgagcgt gtcgtgaact tgagtcagca ggtgcagcta ttagccggac
      241 aagtcgaccg cttgagtcgg ttatttatca cgaacaacag ataagggatg aacaggagcg
      301 tcaggcgcag gagcttgccc ggcgctggca tgaagagcag gaacccgaag cgcggatctg
      361 gcgtggcccg tcaatgggga tgtgaccggg gtgccccctg aaatcgagcc agtttgtggc
      421 acgccctacc cggatggcgc caatgttaat gtacgtacat tagcattaac aatatccaaa
      481 cctccccatt ttgccccgtt cgacggctgg aacgtgcgaa actgatcgtc attcagtttc
      541 gcgcataact atgcatgagg ttaaaattta ccaggcgcga tcgcggcagt ttttcgggtg
      601 gtttgttgct gtttttacct gtctgctgcc gtgatcgcgc tgaacgcgtt taagcggtac
      661 gcgcaatgcg acgttatggt aaatacggag tattttaatt gcgggaatta aaacaacaaa
      721 ggcccgctgt actctataca gcgggccttt gttgtatatc tctgtcgttt gtccccgggg
      781 gggaagcatt actgctaaca agtcccgtga gaaaactgcg caatcgtatt gcgcaccatg
      841 aacctattct caaccgcaat ctggaggatg attttgctac gataaagaga attatcgcgt
      901 atcggtgtca gcattcattg gagtggatgc tgaaaaacca ggtgttactc cctctactta
      961 cacttaaacc tctctaaaaa tataagtaag tactatcgag ggctatcatg aactatggtg
     1021 ccctctgagc atagctaccc attacttctt ctatgtcatt gaataattca tttcttaact
     1081 ctaagtattc tagtaactcc atttctaatg actctgtcgt gaacttaaat ctagaaggat
     1141 ctttgattga gtttactagt cttgataacg ctctatatgt tttgagtgaa ggttgcatta
     1201 tagatggaaa gtatttgaaa acctcttcat ctctgttgaa attaactaaa aacttgtagt
     1261 catcatttat aaatctcaag catacagcgt caagcttatc gccttttctt attattattt
     1321 ctttatagga aagatatttt tcagaagata agtcccacat caagcgaaaa tctctctttg
     1381 cgaacgagtt cgcgtcatca ttgaaaagag atgatgtgat ggctttggat agaccttctg
     1441 tgtttatctc tttgttggat gggtagtaaa ttttattaat tgctttcatt ctctcaaatg
     1501 atgaatatag gtcattatgc caccttgttg aaatcacccc tttagttata ttctccttgc
     1561 taaaattaag ccctgtatca aaaataaatg cagccatcag tgaatatgat atgtttttta
     1621 ttaactcaga cttaaatggt tccgctatgg agtagtacag tgataaaata actaaaaata
     1681 aaaggtattg tcttaccttt cccgatgagt atatgtaaag gcgatattta tttattgata
     1741 gttttatttt gtatggaatg atctcatttc tgtcatatcc tcttcgtggt ggagcaaaac
     1801 aaaagaaaca tgcagctatt aataaaacaa aaaagatgaa cagtgtaagc aagttcggct
     1861 cctgtgtatg ctcactatgc atgattgtct tgagatctaa cgatcctgaa ggtagtcttg
     1921 tccgtaaacg aaaaaaccgc ccagcaaggc ggcttttcga aggttctcag agcagcaact
     1981 ctttgaacca aggtaactgg cttggaggag cacagccacc aaatctgtcc tttcagttta
     2041 gccttaaccg gcgcataact tcaagactaa ctcctctaaa cggttaccaa tggctgctgc
     2101 cagtggcgtt tttacgtgtc tttccgggtt ggactcaaga cgatagttac cggataaggc
     2161 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2221 caccgggccg agataccaac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2281 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2341 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2401 gcgtctgttt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcggcgc
     2461 ggccttcttc ctgttcctgg tcttttgctc acatgtttct tttggcgtta tcccctgatt
     2521 ctgtggataa ccgcatcacc gtctttgagt gagctgatac cgctcgccgc agccgaacga
     2581 ccgagcgtag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg cattttctcc
     2641 ttacgcatat gtgcggtatt tcacaccgca tatggtgcac ttttcttata atccgcactg
     2701 atgccacata gttaagccag tatacactcc gctatcgcta cgtgactggg tcagggctgc
     2761 gccccgacac ccgctaaaac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     2821 cgctcacagg caagctgtga acgtctccgg gagctgcatg tttcagttgc tttcaccgtc
     2881 atcgccgaag cgtgcgaggc aactacggta gagttcgtcg gcgtggtcgt gaagtgattc
     2941 gcagatgtct gcctgttcgt ccgcgtccag ctcgttgagt ttttccagca gggtcagcgt
     3001 ctggcttctg ataaagcggg ccatgttaag ggctgttttt tcctgtttgg tcatggtctt
     3061 aaggctcctt aataggggat tttttagggg gaatgatacc gatgaaacgc gagaagatgc
     3121 tcacgatacg ggttagcgat gaggagcacg cccggttact ggaacgctgc gagggtaaac
     3181 gactggcaga atggatgcgg cgggtctgtc tgggggagcc ggttgcgcgc acaggaaaac
     3241 tgccgacact atcgccgcca ttgctgcggc atctggctgc catcgggaac aatctgaacc
     3301 agacagcccg gaaggtgaac agcggccact ggtcttcgat tgaccgggtt catgtggtgg
     3361 ccgcactgat ggccatcgag ggtgaattgc gccagttgcg gcaggctgtc agggaacagg
     3421 ggggacggga tgatagttaa atttcatgcc agggggaaag gcggtggaag tggcccggtc
     3481 gattacctgc tggggcggga gcgtaaccgc gaaggcgcaa cggtgctccg gggtaacccg
     3541 gaagaaatcc gggaactcat tgatgccacg ccattttcga agaaatacac gtcaggcgtt
     3601 ctgtcgttcg cggagaagga gttaccgccg ggtgaacgcg aaagagtgat gacgagcttt
     3661 gagcgtgttc ttatgcccgg tctcgaaaaa gaccagtaca gcattctctg ggtggaacac
     3721 caggataagg ggcggctcga actgaatttt gtcattccga acatggaact acagagcggt
     3781 aagcgcctcc agccgtacta cgaccgtgcg gacaggccga ggataaatgc ctggcagacg
     3841 ctggttaatc atcattacgg gctgcgtgac ccgaacgcac cggagaacag acggacgctt
     3901 gtcacaccga ataatctgcc aaaagcgaaa caggaggccg cagaagcgat tacacggggt
     3961 cttgaggcgc tttaccgtgc cggagcaata aaaacgcgtc aggatgtcac tgaggcgctc
     4021 acagcggcag gttttgaggt ggtgaggaca accagaagca gtatcagtat tgccgacccg
     4081 gagggggggc ggaatctcag gctaaagggg gcgctgtatg agcaatcttt tacaaatggg
     4141 aacagacttc gagaagaaac tgaaagagcg agccgcttct accgagaaca tgctgaacag
     4201 cgagttcagg aagctagaag agtctgtaga caaggcactg agcttaaaca ggcagaaaat
     4261 cagagacgct atcagcgaac atacaacatc cgtgaaacag caactggata cgctgagcac
     4321 caccgtgagc acgcagctca gcaccacaga ggccgaactg tcccggcagc agaaaaacct
     4381 gctctggcag gtgatcaaag ggcgggtact gtttccggcg ctgaccgcgc tcagcgtcac
     4441 gggcggcatt ttcctggggt gctgggggct gattcagtgg caggaaagca ggattgcgaa
     4501 aaatatcttg accatcagag agcaggaaaa cacgctggcg aaactcgagg caaaaacctg
     4561 gggggtgacg ttcgtcaatg gcgagaacgg gaaatttctg gtgctgccgg acggggtgaa
     4621 aggggaaaac acct
//
