LOCUS       CP016039                5775 bp    DNA     circular BCT 01-SEP-2020
DEFINITION  Escherichia coli isolate Co6114 plasmid pC06114_5, complete
            sequence.
ACCESSION   CP016039
VERSION     CP016039.1
DBLINK      BioProject: PRJNA321990
            BioSample: SAMN04963292
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Both,A., Huang,J., Kaase,M., Hezel,J., Wertheimer,D., Fenner,I.,
            Gunther,T., Grundhoff,A., Buttner,H., Aepfelbacher,M., Rohde,H. and
            Hentschke,M.
  TITLE     First report of Escherichia coli co-producing NDM-1 and OXA-232
  JOURNAL   Diagn. Microbiol. Infect. Dis. 86 (4), 437-438 (2016)
   PUBMED   27681362
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Both,A., Huang,J., Kaase,M., Hezel,J., Wertheimer,D., Guenther,T.,
            Buettner,H., Aepfelbacher,M., Rohde,H. and Hentschke,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2016) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-APR-2016
            Assembly Method                   :: SPAdes v. 3.8.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 192.0x
            Sequencing Technology             :: Illumina MiSeq; Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/14/2016 13:43:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 3.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,155
            CDS (total)                       :: 5,053
            Genes (coding)                    :: 4,954
            CDS (coding)                      :: 4,954
            Genes (RNA)                       :: 102
            rRNAs                             :: 8, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 34 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 8 of 99
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Source bacteria available from Moritz Hentschke, MVZ Labor Dr.
            Fenner und Kollegen, Bergstrasse 14, 20095 Hamburg, Germany.
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="Co6114"
                     /db_xref="taxon:562"
                     /plasmid="pC06114_5"
                     /geo_loc_name="Germany: Hamburg"
                     /collection_date="2015-05-01"
     gene            join(4309..5775,1..87)
                     /locus_tag="A8V37_25690"
     CDS             join(4309..5775,1..87)
                     /locus_tag="A8V37_25690"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="ANM85916.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGDPEEVRELID
                     ATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLE
                     LNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNL
                     PETKQALAESVTRGIDALYHVGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGKN
                     IRLKGAFYEQSFTDGRGVREKAERESRIYRDNAERRVQQARKICKQGCDIKRDENQRRY
                     SPVHSFDRGITEKTPGRGERGDDAAQEGRVKAGREYGHDVTGDGHFPVYREWRDALVSW
                     RDDTDEPGRNQDTGRNIAETEREDMGRGVCAGREKEISGHPAGEIGNGDSLSGEGLGTT
                     DGVTQSDGAGKTFAERLRATATGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAV
                     ESAGAALERADRTLEPVIQREQEIRDERLIQEREHELSLERERQLEIQERTLDGPSLGW
                     "
                     /name="nuclease"
     gene            406..552
                     /locus_tag="A8V37_25695"
     CDS             406..552
                     /locus_tag="A8V37_25695"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_000266281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein"
                     /protein_id="ANM85911.1"
                     /translation="MWLKFTGCDRGSFSGGLLPFLPVCCRDRTERVSAVRATDVMVNIG
                     ALG"
                     /name="plasmid mobilization protein"
     gene            1186..1698
                     /locus_tag="A8V37_25700"
     CDS             1186..1698
                     /locus_tag="A8V37_25700"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_001445155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ANM85912.1"
                     /translation="MSARARKAGKRNQSERRQDLIEKLWEDDEIDTWHRLENDGYSTVP
                     RYLPLIGDLMDGLSKGSPLSTTYLALWFRVSDEGLIEIRDKAALAFESGFASERGVTTW
                     AGRMKKLKELGFISCREGSTGEFHYVLIVHPLVAVKKLLDEGIIPKGKTYNILSERVIE
                     VGASWEG"
                     /name="hypothetical protein"
     gene            1699..2193
                     /locus_tag="A8V37_25705"
     CDS             1699..2193
                     /locus_tag="A8V37_25705"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_000929317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ANM85913.1"
                     /translation="MAGTVWRDIRTGETVFPMGHLHPQRCCVDVNGTSVDIEISFGFHV
                     FTDEKQTGMLMKFKEEQRFFCRERYEGSKTIVHRILTAIENGEYITAFISKGQGQRYYH
                     LSHHDDFILMEIRKPQDRNNSLRIHVVTAYTLDEWGTVNKGRNLRFRYVLEQRLQGKKI
                     V"
                     /name="hypothetical protein"
     gene            2226..2768
                     /locus_tag="A8V37_25710"
     CDS             2226..2768
                     /locus_tag="A8V37_25710"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ANM85914.1"
                     /translation="MQNFGLAFRYPAHRLGGCDPRHQHPSLEVPVCADNISILFHMFVV
                     KLAYFLVFIGVYRLSFTLFVVISVYRFSLFFIGVHMKLLDSNEVCELLGISKSTLYRWC
                     GVSEDFSGLGLSSGLPQANATQNSLGRENRLVRRELQQNALGVEETPSDFPRPFKIGRS
                     FKWKDAEIMAWLETRRV"
                     /name="hypothetical protein"
     gene            complement(3740..3931)
                     /locus_tag="A8V37_25715"
     CDS             complement(3740..3931)
                     /locus_tag="A8V37_25715"
                     /inference="EXISTENCE: similar to AA
                     sequence:SwissProt:P03051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ANM85915.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="hypothetical protein"
     gene            3996..4319
                     /locus_tag="A8V37_25720"
     CDS             3996..4319
                     /locus_tag="A8V37_25720"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="ANM85917.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSEQWSSGDRVQVVAALMAIERELRSLRQVVREHGAR
                     DDS"
                     /name="mobilization protein"
     Typing          3565..3648
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.07e-36"
     Typing          2902..3123
                     /name="rep_cluster_2392"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.825"
                     /coverage="87.0"
                     /evalue="1.42e-85"
     Typing          4310..5775
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.467"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 gagcgcgaac atgagttatc cctggagcgt gagcgccagc tggaaataca ggaacggaca
       61 ctggatggcc cttcgctggg atggtaatca gagacaggct aaaatgttgt cagcgcgttt
      121 ccggtgcatt ttttgggata atggttggtt cgggggcacc gggaacgcgc ttcagtcaga
      181 ccttgctgaa aacacaggaa cgcgttaaaa cgcatacggg cgcgattctg gtgtgtcttt
      241 tgttgctgtg ttttgcctct attgccagat cgcgctcatt cccctgaact tctgttttcc
      301 cgctccggtc cccgtcttct tcgcttccga aagcatgaaa aaaatccgtt tcagcagtct
      361 ctggctggca attcctgcgt gattcacatg gctgcatagc tatgcatgtg gttaaaattt
      421 accgggtgcg atcgcggcag tttttcgggt ggtttgttgc catttttacc tgtctgctgc
      481 cgtgatcgca ccgaacgcgt ttctgcggtg cgggcaacgg atgttatggt aaatattggt
      541 gctctgggat gaagcgaaaa taggcttttt ttcgctattt tagcctgttc aggaacggca
      601 agagtaaata tctgggagca tgaagctcgt taacctgaat ggagatcctg atgatggaca
      661 aagataccac caccctaaaa cgcactttgg ctcataatcg agcctttagc gataatatta
      721 accgcagtgg catagcctgg tgctataaca ctgagattgt actcgctgct tgtgaggcga
      781 ttgaagccga actacaacgg cgaggatgtt tgtaacaata agaaaacccg catcagcggg
      841 ttttttagtc tctgagacgc gctaccgcta aattattttt ctctctatcc gccctggctt
      901 gccccctaca accccatgga gcacaaggag cacagggaga accatcgagc acaacgagca
      961 caatgagcac cgccttcctc ttataaatca ttattaatca tataattaaa caacttcatt
     1021 gtaatatata ttacttttta tgtttttaaa tgtaatgttt attgcgattt agcttgtttt
     1081 taactctatt ttttcagatg agaacaagct gccagcaatg aataactatt tgattttatt
     1141 ggttttttca ataaattagt gtccatagac aaacgggaga cgactatgtc cgcaagagca
     1201 agaaaagcag gtaagagaaa tcagtcagaa cgacgtcagg atttgattga aaaattgtgg
     1261 gaagacgacg aaatcgatac ttggcatcga cttgaaaatg atgggtactc aacagttcca
     1321 cgttatttgc ctttgatagg cgatctcatg gatggtctgt caaaaggatc accattatca
     1381 acaacctact tggctttatg gttcagggtt tctgatgagg ggttgattga aataagagat
     1441 aaagcagcac tggcttttga atcagggttc gctagtgaaa gaggcgtaac tacatgggca
     1501 gggagaatga aaaagctaaa agaactgggg ttcatatcct gtcgtgaagg cagcaccggt
     1561 gaatttcatt acgtgttaat tgttcaccca ttagttgcag tcaaaaagct gcttgatgaa
     1621 gggattatcc ctaaaggaaa gacttataac attttgtcgg agcgtgtaat tgaggtggga
     1681 gcctcttggg agggttaaat ggcgggcaca gtatggcgtg acataagaac gggagagact
     1741 gtttttccta tggggcacct gcaccctcag aggtgctgtg ttgatgtaaa cggtacttct
     1801 gtagatatag aaattagttt tggttttcat gtttttactg atgaaaaaca aaccggaatg
     1861 ctgatgaaat ttaaggagga acagaggttt ttttgtaggg aacgctatga gggcagcaaa
     1921 actatcgttc acagaatact tacagcgata gaaaacggtg aatacattac agcatttatt
     1981 tcaaaggggc aggggcagcg gtattaccat cttagccatc atgatgactt cattttgatg
     2041 gaaataagaa agcctcaaga tagaaataat tctttgcgta ttcatgttgt tacagcttac
     2101 acgcttgatg aatgggggac tgtaaataaa ggacgtaacc tgcggtttag atatgtgctg
     2161 gaacagcgac tgcagggaaa gaagattgtg taaaaaacaa aaagcgccta aggcgccttt
     2221 gttttttgca gaatttcggt ctggcctttc gttatcccgc tcacaggctg ggagggtgtg
     2281 acccccggca tcagcacccc tctctcgagg ttccggtttg tgctgacaac attagtatcc
     2341 tattccacat gttcgttgtc aaactagcgt actttttggt gttcattggt gtttataggt
     2401 tgtcatttac cctctttgtg gttataagtg tttataggtt ttcattgttt tttataggtg
     2461 ttcatatgaa gttgcttgat agtaatgaag tgtgtgagct cttaggcatc agtaagtcca
     2521 ctttgtatcg ttggtgcggg gtgtctgagg atttttctgg cttgggtcta agtagcggtc
     2581 ttcctcaggc taacgctact cagaactctt taggcaggga aaataggctc gttaggaggg
     2641 aattacagca aaacgctctc ggtgtcgaag agacgccaag cgacttccct cgtcctttca
     2701 agatcggtcg ttcgtttaaa tggaaagacg ccgaaattat ggcttggctt gaaacgcgac
     2761 gcgtttgatt gatagatctt cttgagatca tttggatctc gtcgtagtct tttgtcctgt
     2821 aaacgaaaaa accaccatgg caggtggttt ttcgaaggtt atttaatcct ggcagattga
     2881 ataaccgtgg taacagcctt gtgcgagacg tgtcaccaaa tctgtccttt cagtgtagcc
     2941 tcactaaggc caccacttca agaactttga atacatctct cgcacatcct gtttaccaat
     3001 ggctaccgcc actggcgatt tttcgtgtct gttccgggtt ggactcaaga tgatagttac
     3061 cgggacaacg tggtagtcgg gctgaacggg gggttcttgc atacagccca gcttggagcg
     3121 aactgtctaa acggaacggg acgtggtgat ttaggtaaag cttccaccac aacacggatg
     3181 ccgcaggacg ggaacaggag agcgcaagag ggagccgcca gggggaaacg cctggtatct
     3241 ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3301 ggggggcgga gcctatggaa aaacgccagc ggcgtggcct tctctgggtt cttgtttttt
     3361 gctcacatgt ttcttttggt gttatcccct gattctgtgg ataaccgcat taccgtcttt
     3421 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gtagcgagtc agtgagcgag
     3481 gaagcggaag agcgtcggaa gttgcatttt ctccttacac atctgtgcgg catttcacac
     3541 ccggcatggc gcacttttca tacaatccgc actgatgccg catggttaag ccagtataca
     3601 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcaa ggtcaacggc
     3661 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc tcacagacaa gctgtgaccg
     3721 tctccgggag ctgtatgtgt cagaggtttt caccgtcatc cccgaagcgt gcgaggcagc
     3781 tgcggtaaag ctcatcggcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg
     3841 cgtccagttc gttgagcttt tccagcagcg ttaatgtctg gcttctgata aagcgggcca
     3901 tgttaagggc ggttttttcc tgtttagtca ctgatgcctc cgtgtaaggg gggatttctg
     3961 ttcatggggt aatgataccg atgaaacgcg agaggatgct cacgatacgg gttactgatg
     4021 atgaacatgc acggttactt gaacgctgtg agggtaaaca actggcggta tggatgcggc
     4081 gggtctgcct gggggagccg gttgcccgtt ccgggaagct gccgacactg gcaccgccgt
     4141 tactgcgtca gctggccgcc atcgggaata acctgaatca gacggcgagg aaggtgaaca
     4201 gcgagcagtg gtcttccggt gaccgggttc aggtggtggc cgcactgatg gccatcgaga
     4261 gagagttgcg cagcttacgg caggtcgtca gggaacatgg agcgcgggat gatagttaag
     4321 tttcatgcca ggggaaaagg cggtggcagt ggtccggttg attacctgct gggaagggag
     4381 cgtaaccggg aaggggcaac ggtgcttcag ggtgatccgg aagaagtccg ggaactcatc
     4441 gatgccacgc catttgcgaa gaaatacacg tcaggtgttc tgtcgttcgc ggagaaggag
     4501 ctgccgccgg gaggacgtga aaaggtgatg gcgagctttg agcgtgtact gatgcccggt
     4561 ctcgaaaaga atcagtacag catcctgtgg gtagagcacc aggacaaggg acggcttgag
     4621 ctgaattttg tcattccgaa catggagcta cagaccggaa aacgcctcca gccgtactac
     4681 gaccgggcag acaggccgag aattgatgcc tggcagacgc tggtgaatca ccattacggg
     4741 ctgcatgacc ccaacgcccc ggagaaccgc cggacgctga cccttcctga taacctgccg
     4801 gaaacgaaac aggcgcttgc agagagcgtc acacgcggga tagacgctct ttatcatgtc
     4861 ggggagataa aaggccgtca ggatgtgatt caggcgctca cagaggcagg gcttgaagtg
     4921 gtcagggtga cgcgaagcag tatcagcatt gcagacccga acggtgggaa gaatatcagg
     4981 ctgaaaggag cattttatga gcaatctttt acagacgggc gcggagttcg agaaaaagct
     5041 gaaagagaga gccgaatcta cagagacaat gctgaacgac gagttcagca agctcgaaaa
     5101 atctgtaagc aaggctgtga catcaaacga gacgaaaatc aaagacgcta tagccctgtt
     5161 cacagcttcg acagaggaat cactgaaaaa acaccgggaa ggggtgaaag aggcgatgat
     5221 gcagcacagg aaggacgtgt taaagctggc agggaatacg ggcatgatgt tactggggat
     5281 ggtcattttc ctgtttaccg tgagtggcgg gacgctctgg tatcttggcg ggatgataca
     5341 gacgaacctg gaagaaatca ggatacagga agaaacattg cagaaactga acgcgaagac
     5401 atggggcgtg gagtttgtgc aggacgggag aaggaaattt ctggtcatcc cgcaggggaa
     5461 atcggcaacg gtgattccct atcaggggaa ggactgggta caactgacgg agtgacacag
     5521 agtgacggag ctggaaagac atttgctgaa cgccttagag caactgcaac aggactatat
     5581 gcagcggctg aacgaatggg agagcgcctt cgtggaattg cagaaaatgt tttcgcttac
     5641 gcaacaggac aacgcgatgc tgaacgagca ggtcatgcag ttgagtcagc aggtgcagca
     5701 cttgagcgag cggacagaac gcttgagcca gttatacagc gagaacagga gataagggac
     5761 gaacggctga tacag
//
