LOCUS       CP016038                6141 bp    DNA     circular BCT 01-SEP-2020
DEFINITION  Escherichia coli isolate Co6114 plasmid pC06114_4, complete
            sequence.
ACCESSION   CP016038
VERSION     CP016038.1
DBLINK      BioProject: PRJNA321990
            BioSample: SAMN04963292
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Both,A., Huang,J., Kaase,M., Hezel,J., Wertheimer,D., Fenner,I.,
            Gunther,T., Grundhoff,A., Buttner,H., Aepfelbacher,M., Rohde,H. and
            Hentschke,M.
  TITLE     First report of Escherichia coli co-producing NDM-1 and OXA-232
  JOURNAL   Diagn. Microbiol. Infect. Dis. 86 (4), 437-438 (2016)
   PUBMED   27681362
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Both,A., Huang,J., Kaase,M., Hezel,J., Wertheimer,D., Guenther,T.,
            Buettner,H., Aepfelbacher,M., Rohde,H. and Hentschke,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2016) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-APR-2016
            Assembly Method                   :: SPAdes v. 3.8.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 192.0x
            Sequencing Technology             :: Illumina MiSeq; Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/14/2016 13:43:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 3.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,155
            CDS (total)                       :: 5,053
            Genes (coding)                    :: 4,954
            CDS (coding)                      :: 4,954
            Genes (RNA)                       :: 102
            rRNAs                             :: 8, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 34 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 23 of 99
            Pseudo Genes (multiple problems)  :: 8 of 99
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Source bacteria available from Moritz Hentschke, MVZ Labor Dr.
            Fenner und Kollegen, Bergstrasse 14, 20095 Hamburg, Germany.
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="Co6114"
                     /db_xref="taxon:562"
                     /plasmid="pC06114_4"
                     /geo_loc_name="Germany: Hamburg"
                     /collection_date="2015-05-01"
     gene            249..641
                     /locus_tag="A8V37_25660"
     CDS             249..641
                     /locus_tag="A8V37_25660"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_005671614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ANM85905.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="hypothetical protein"
     gene            complement(671..943)
                     /locus_tag="A8V37_25665"
     CDS             complement(671..943)
                     /locus_tag="A8V37_25665"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ANM85906.1"
                     /translation="MVQFLQLAAYSGQLRYDAQPIQRPPLPGINAPGDLVQVVAHSGQL
                     PQERRIYGRRRRSALGLRNTETLLPHPLRQLGLGAELPQALKLRI"
                     /name="hypothetical protein"
     gene            942..2396
                     /locus_tag="A8V37_25670"
     CDS             942..2396
                     /locus_tag="A8V37_25670"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_019820366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ANM85907.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="hypothetical protein"
     gene            complement(2678..2959)
                     /locus_tag="A8V37_25675"
     CDS             complement(2678..2959)
                     /locus_tag="A8V37_25675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ANM85908.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            3303..4100
                     /locus_tag="A8V37_25680"
     CDS             3303..4100
                     /locus_tag="A8V37_25680"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="ANM85909.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="class D beta-lactamase"
     gene            5035..5982
                     /locus_tag="A8V37_25685"
     CDS             5035..5982
                     /locus_tag="A8V37_25685"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicase"
                     /protein_id="ANM85910.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replicase"
     ARG             3304..4097
                     /name="blaOXA-232"
                     /gene="blaOXA-232"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          5048..5969
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          943..2393
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcagttatcc acggagccgc tctttccctt gatcttgctc tagggcttgc agtatcccgc
       61 ccatggttga ataatgtact aagcaaggct acacagaggc ccctctacct gcttcgcggt
      121 ctcgggcctc tgtggccttg ttcaggggtg ctacgccccc ccgaaacccc ctgtggcccc
      181 cgctcgatcc gccccttcgg gacggtctgc gcggtggctt cttggctctg gagaatggac
      241 gaggccctat gtcaacaaca cagatagaga aagattcgag aaggaaggca gtcaatggcg
      301 ttatttctag tttccaacta gagggggagg ccataaccga tgaattcaaa attctacttg
      361 aagattatat caatggaaaa atttccaccg atgaggtgtt agctatggta agtgacgaac
      421 ccacaacaac ggaatacgac tacgaaatca ccccccgcac tctcggtctt ggcgggggct
      481 ggaatctgcg tttgctggag aacggcgaag aggtcggcgg gggtgttttc cccttgccgg
      541 agcactgcga ttttcgggat gaaaaggcgc tccagacgct actcgatagc ctctatgagg
      601 acgccttagc ggaggcctct gcatggctgg ccagccgtta aagcgcctcc gctctatcaa
      661 gatccgggtc tcagatgcgg agcttgagcg cctgcgggag ctctgcccca aggcccaact
      721 ggcggagtgg atgcgggagc aatgtctcgg tgttgcgcaa ccccagcgcc gaacgccggc
      781 gcctaccgta gatccggcgc tcttgcggca gttggccgga atgggcaaca acctgaacca
      841 gatcgcccgg cgcgttaatt ccggggagtg ggggccgttg gatcggctgc gcatcatagc
      901 ggagctgtcc gctataggcc gcgagctgga ggaactgcac catgatcatc aaattccata
      961 accgtggggt aggccggggc tccgggcctg ttggctactt gttgggccgt gacgggaacc
     1021 gggaaggcgc aacgctcgac cggggcaacc ctgcggtgat tgaagccctg attgattcca
     1081 gtccctacgc caaaaaatac acctctggcg ttctgtcgtt tgccgaggct gatttaccca
     1141 gggcgaccaa ggacaagctg atgagcgact ttgaaaaggc gctcctgccc ggactggagg
     1201 gcaaccagta cgctgttttg tgggtagagc accgcgacaa ggggcggctg gagctgaatt
     1261 ttgtagtccc gaacatcgaa ttgcagagcg ggaagcggct gcaaccctac ttccacaagg
     1321 ccgacaatcc gcgcctcgat gcgtggcgaa ccgtggtaaa cggggagctg aaattgcacg
     1381 acccggacga tccgttaaac cgtcaggccg ttatcacgcc aaaggatttg ccgagggggc
     1441 acaaggaggc cgctcaggcc attacagacg ggcttttacg catggccgga gcaggggaga
     1501 tccagagccg cgatgatgtg cgcaaagcct tgcagggtgc gggtttcagc gtggtacgcg
     1561 aaaccaaaaa cagcctgagc attgccgacc cggacggggg gcggaacatc cgattaaagg
     1621 gggtgctgta tgagcaagat tttagacatg gcgaaggact tcgagcagag ctcgaagcag
     1681 caggccaacg ctatcgagaa gcgtctgacc gaagaattca cgaggcacga gaaagctatc
     1741 tccggggcgt tgaaatcaag cgaggagaaa atgagcgccg ctattgccga gcacagccag
     1801 aaaatgggct ggctgttgct caaaacgtgg atctggatgc tggtcagcat cgtcctgttc
     1861 ttgagcgcaa cgagcgggat cctctggtat cagggggaat tgatagcgga gcggcaagcc
     1921 actctccaag ccttggcaga gaagggcggc aagctaaagc tgacccagtg cgggaagaaa
     1981 ttggagcacc tttgcgtgca gatctccgac cccaatccaa ccgtttgggg gaaggatacc
     2041 tattggatcg tgccgaaggg gtactaaatg acgggattgg agcgggaatt gctctgcgcg
     2101 ttcgaggctt tgcagagcgc atacgagcag cagcacaagg catggcagga aagctacgcg
     2161 aacttggcga gcatgtacga gagcaccaaa caagaggccg agatcagcag ggcagcagca
     2221 gagcgattga cgaggcaggt gaacggctta acgcagcaag tggacggctt gagcaagcag
     2281 ctggagggct tgaccgggct agtcagcaag ttgcaaaaga aagggaacac aggctcctga
     2341 aggcactgga tcgaggctgg gagcgtggag acagatcgcg aggctttgag cgttgacgga
     2401 acaaaacggc cgtcagttat aaaaaaagga aagtgctatc actttccttt tatcggcggg
     2461 aattatcgtt gagttcttct atgccatttt tcatagtagt aactgtaatc ttcatctcta
     2521 ccaccagatt caatccagtc atcatactca gggctatttt tgaaatctga atcagacatg
     2581 ctaactcctt gttattagtt ggcttctatg gaagctagcg caccgtgaag ttatgatcaa
     2641 cgatgtagtc cacatcattg tcgaaatgtg cgttcagcta gtcttcggtg ttttctgctt
     2701 tatttctagc ggcgattctc gcgtctgccc atgcggcaga ctttttagcc tgttctcgtt
     2761 gttcttcggt tgaagactcg ctgttgagaa ctttgttgct cgtttgtttg accaccgaat
     2821 ccaaacctgt tgttgctgca aaatgagcta ggttaacaac tcccttacct aacaacttgg
     2881 cactagcgat tgcggtttct ttagccccaa aagcatcaga cacctcctca agcgctttag
     2941 ataggttaaa aaatcccata ctccccctaa gaatatcatc aataaaattg agtgttgctc
     3001 tgtggataac ttgcagagtt tattaagtat cattgcagca aagatgaaat caatgattta
     3061 tcaaaaatga ttgaaaggtg gttgtaaata atgttacaat gtgtgagaag cagtctaaat
     3121 tcttcgtgaa atagtgattt ttgaagctaa taaaaaacac acgtggaatt taggtaatag
     3181 ttaaagcttg tgcctgattg ttgccacaat tggtcagcag tttgctaggg aatgagaggg
     3241 cagagaatga taagcttagc acttaatttg tatggatata ttgcattaag caaggggacg
     3301 ttatgcgtgt attagcctta tcggctgtgt ttttggtggc atcgattatc ggaatgccag
     3361 cggtagcaaa ggaatggcaa gaaaacaaaa gttggaatgc tcactttact gaacataaat
     3421 cacagggcgt agttgtgctc tggaatgaga ataagcagca aggatttacc aataatctta
     3481 aacgggcgaa ccaagcattt ttacccgcat ctacctttaa aattcccaat agcttgatcg
     3541 ccctcgattt gggcgtggtt aaggatgaac accaagtctt taagtgggat ggacagacgc
     3601 gtgatatcgc cgcttggaat cgtgaccatg acttaattac cgcgatgaag tactcagttg
     3661 tgcctgttta tcaagaattt gcccgccaaa ttggtgaggc acgtatgagt aaaatgctgc
     3721 acgccttcga ttatggcaat gaggatatct cgggcaatgt agacagtttt tggctcgatg
     3781 gtggtattcg catttcggct acccagcaaa tcgctttttt acgcaagctg tatcacaaca
     3841 agctgcacgt ttctgagcgt agtcagcgca tcgtgaaaca agccatgctg accgaagcca
     3901 atggcgacta tattattcgg gctaaaacgg gatactcgac tagtatcgaa cctaagattg
     3961 gctggtgggt tggttgggtt gaacttgatg ataatgtgtg gttttttgcg atgaatatgg
     4021 atatgcccac atcggatggt ttagggctgc gccaagccat cacaaaagaa gtgctcaaac
     4081 aggagaaaat tattccctag aagtggttag cgcgtatttg tgtgaaatag ccgtcatata
     4141 agctgtaaag atatatggac aaaatgctga tgctcgatgc gcttgtagtt aatggctcat
     4201 acttaatcgg tcaggcctaa tcgacttaag ctccgcgcgt taactttcag cctgagcatt
     4261 agcatcacat tcctaaacgt aaaaagccta gccaatttgc taggcttttt tgttatctca
     4321 atggccatgg tcactcaaca tgggggtgtg aataaaggag tggcgatgtc agtaaaggtg
     4381 aatgccgctg cgataaagtg cctagcccat caaggattgt aggctttctt ggctgctaaa
     4441 gcgtttgacg aaaaagtcca agaaggcgct gatattggtt ctccatcatt ttcatctacc
     4501 ggtttactat aggtcaacca ggccgtcctt gccggcgctt gggacgcaga cgatggcatc
     4561 aaatgcctcg ctcaaattcg tttctacaga ggcgctttgg gaccgcattc gctttgcttt
     4621 catgggggca tctgtcaatg tcagacatga atcctccgat ataggttgat ttttgatctc
     4681 ttcaacactt ggtgttgtcc gactagagag acttatatgg atcagtattc ttgcactgac
     4741 aatggtgcca ctcaatccca aggatcgacc tagttagagg cgtagatcaa gcaattcaaa
     4801 acagcgcaga agcagaagga ggtggaaata tgttccaggg tgaaaggtta catgcaacta
     4861 acgttgctct ctaggatgtg cctgctgtaa cgtattgtcc agaaaagaca accccccgtt
     4921 atcgcttgca tcttggcgga gctgcgaaaa acgggggccg tgacgaatca caagagaagt
     4981 ctatcacatg gatcatgcca aacaacccca cgccgccaat aagcgcggtg gtgcttgaga
     5041 aagtgcgcac agcttgagct gtttcgggag cgcctgcccc ggaaacccta ctattccgac
     5101 gagcttacaa cgggcctacg gattgcggat gttgcccgcg cccttggtgc gcgttacatc
     5161 cagccgaacg ggccaacgca ccgccactgg atcgtttttg acgtagacca tgcagccgct
     5221 acgttatctt gggatgacgt aggcgctcca gcccctaaca ttaccgtcac gaacaaggcc
     5281 aacggccacg cgcatctgat ctacggactg gatacgccga tccgcactgc cccggatgga
     5341 aatgcagccc ctctgcggta tgccgccgcc atcgaggccg cgctccggga gaaattaggg
     5401 gcggatatgg gctactccgg gctgatctgc aagaaccccc tgcatgagca ctggctggtg
     5461 caggtatggg aaccccggct ctacgattta gcctggctat ccgattacct cgatctgagc
     5521 tcctacaatg gccgcaaatc gcttcctgag tacgggcttg ggcgaaactg taccctgttc
     5581 gagaaaacgc gcctgtgggc ctataaagcg atccggcagg gctggccgga ttaccccgaa
     5641 tggttggccg cttgtgttga ccgggcatca ggatataacg cccagttcga gcagccgctc
     5701 cccgcaaacg aagtccggca cacggccaag tccattgcca aatggacgca ccagcaccta
     5761 agccccgcag ggttcagaga ggagcaggcc cggagaggtg cgaagggcgg caaggtatcc
     5821 aagggagggg gaaggccgag caatagcggt aaggacaagt ccgatctatt gccggaggtt
     5881 ctgcggctta aggctcaggg gtacaccaac cgcgatatag cggacgatct tcaaatctcc
     5941 cccagtacgg tgagcgttta cctaaagcgt gatcgtccgt gatcgaaaca agccttatca
     6001 gataatagcc ccctttaggg gggttcctgc gctcttgtct tttagcctta aaaaatcctt
     6061 ttagcgcgga tctttggcga ggtggacaac tgcgcctgtc cgtccatcgc ccccctgttt
     6121 tttcgggggg tgtgggcggc a
//
