LOCUS       CP015486               15198 bp    DNA     circular BCT 02-MAY-2016
DEFINITION  Acinetobacter baumannii strain ORAB01 plasmid pORAB01-3, complete
            sequence.
ACCESSION   CP015486
VERSION     CP015486.1
DBLINK      BioProject: PRJNA271775
            BioSample: SAMN03277095
KEYWORDS    .
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 15198)
  AUTHORS   Adams,M., Brinkac,L., Sanka,R., Wright,M., Sutton,G., Bonomo,R.,
            Buser,G., Beldavs,Z., Pfeiffer,C., Cassidy,M., Cunningham,M.,
            Tierheimer,J. and Rudin,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2015) JCVI, 9704 Medical Center Drive, Rockville,
            MD 20850, USA
REFERENCE   2  (bases 1 to 15198)
  AUTHORS   Adams,M., Brinkac,L., Sanka,R., Wright,M., Sutton,G., Bonomo,R.,
            Buser,G., Beldavs,Z., Pfeiffer,C., Cassidy,M., Cunningham,M.,
            Tierheimer,J. and Rudin,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2016) JCVI, 9714 Medical Center Drive, Rockville,
            MD 20850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Assembly Name :: HGAP assembly of Acinetobacter baumannii ORAB01
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.36x
            Sequencing Technology             :: Pacbio RS
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/26/2016 12:10:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision      :: 3.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,965
            CDS (total)                       :: 3,869
            Genes (coding)                    :: 3,684
            CDS (coding)                      :: 3,684
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 166 of 185
            Pseudo Genes (incomplete)         :: 15 of 185
            Pseudo Genes (internal stop)      :: 6 of 185
            Pseudo Genes (multiple problems)  :: 2 of 185
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Source DNA available from Robert Bonomo at robert.bonomo@va.gov.
            This genome has a base modification file available.
FEATURES             Location/Qualifiers
     source          1..15198
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="ORAB01"
                     /isolation_source="bodily fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pORAB01-3"
                     /geo_loc_name="USA: Lincoln County, Oregon"
                     /lat_lon="44.6 N 123.9 W"
                     /collection_date="2012-06-14"
                     /collected_by="OSPHL"
     gene            60..2471
                     /locus_tag="SG90_019750"
     CDS             60..2471
                     /locus_tag="SG90_019750"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_000932942.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ANB90642.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="hypothetical protein"
     gene            2776..3237
                     /locus_tag="SG90_019755"
     CDS             2776..3237
                     /locus_tag="SG90_019755"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /codon_start=1
                     /transl_table=11
                     /product="septicolysin"
                     /protein_id="ANB90643.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="septicolysin"
     gene            complement(3558..4091)
                     /locus_tag="SG90_019760"
     CDS             complement(3558..4091)
                     /locus_tag="SG90_019760"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_005207483.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ANB90644.1"
                     /translation="MLYEAYYPSEGSDNHPIKIFYGFVQEGFEKGHQLTLVVFYPELTG
                     TSLFNLMDETKAGLANFLLQKHVYGVGASHIKVAFVCEHVNKRDFYTEIWGVDVFENYA
                     LEQLEQQPKSLLSGILGNNSKEVSIHSSYLNIGLTDSIKLIDEGHLDTEQTAYLLNKLK
                     EKTDYGNFNKIFGE"
                     /name="hypothetical protein"
     gene            complement(4159..5976)
                     /locus_tag="SG90_019765"
     CDS             complement(4159..5976)
                     /locus_tag="SG90_019765"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_005207484.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ANB90645.1"
                     /translation="MLHNFFMSYYGYLINGLHLKPLFVGFANTLFALAIIVIFTMIVVG
                     IKKIYKAHDPVYEIGYNIVLLAVVVLAHTYLLSYITNLLPNNYVVVPEKNYFFSWAVII
                     GLYVYLYFTGKKSKPRVERIKKESAEGYGTLLSELKRGFENKTPLIAFTDQTLPKKQIK
                     EMEQKYLDWSFHRESLRVTNEDRGIVLGAPGSGKTTFLISQIVEWMKSGRSFVATDVKP
                     EIWAILKANNVFKIFGYTDWVFNPTDVDSDHYNIFSEVKDSAELNEILNIIIKDDTADS
                     VVFNDLARRILKAVLIELGDKASLVNAQNYINSMDSNDELLKALRQSEKSSVSNIAKEV
                     SRTAQNDRLLASVMTAVSKAFEFLDDDRIRVTTSDNTQGFYLKEVLMKPKQAVFLQFDQ
                     QYKSSTATLFGAMVAHTMRILQANQNREAVFIALDEIINCAPIPKFTDLLNTIRSANMP
                     TFLYLQSLEGLNRLYGANSDKMFMGSSNLKIVFRIGDIESAEECSRLVGQTETTYISET
                     AGTSQTSGTSSSSRASSSSSNRSQNTGTTKSIKLESIIEPAEFIKLPICTAVVMYNGSY
                     GTLEMPKYYECYNMPKRTNLKTIRDFKVA"
                     /name="hypothetical protein"
     gene            complement(5977..6216)
                     /locus_tag="SG90_019770"
     CDS             complement(5977..6216)
                     /locus_tag="SG90_019770"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_000510443.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ANB90646.1"
                     /translation="MGIASEIRSSLPLCGRCINCKLLIWNDKAELKSTQNLIKFRSSEA
                     SFYYTVRCSWLKSPVSEPQFLDTCEGKQQQKGSD"
                     /name="hypothetical protein"
     gene            complement(6217..7110)
                     /locus_tag="SG90_019775"
     CDS             complement(6217..7110)
                     /locus_tag="SG90_019775"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_005207487.1"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="ANB90647.1"
                     /translation="MTLEYQFDQLCNKFREGSHATQANRRAMLKLFTEQLAKNGYDVKT
                     MSPKDLKGRHVNKLLEQWRKDGVSTGTIKNRMVAVRWWAEKVGNSGAVKDNATYKIENR
                     VYVTNENKSISLKDLDLSKTDPNIAQSLRLQDAFGLRREESMKFQPEYALDGQKIENAK
                     YIRLKDSWTKGGRPRTIPITNEKQRQELKNAYAQAVKNGGSMIPKEKSYKSHKANFEAV
                     THALGVGQTHGLRHGYAQTRYRELMGFDCPAVGGSRSLSADEQVKDKEIRMLISEELGH
                     SRINITSVYLGSWSKN"
                     /name="integrase"
     gene            8318..9268
                     /locus_tag="SG90_019780"
     CDS             8318..9268
                     /locus_tag="SG90_019780"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="ANB90648.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLDLFSKMTDAQRHMFANKLSELPEMGRYSQGTESYPQFAV
                     RIAEMLQDSEKIKELAPYLKKVGYMPSNKKDTVNG"
                     /name="DNA replication protein"
     gene            9261..9836
                     /locus_tag="SG90_019785"
     CDS             9261..9836
                     /locus_tag="SG90_019785"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="ANB90649.1"
                     /translation="MAKLSLSEVSKKFHIDRSTIYRAVRNGRLSRSSDGQFDLSEVIRC
                     FGEPEQISQQIEPSKSDNDESTKKLIIHLENEVKKYQEREERLMQQIDRMQTLIELKTV
                     APATAAPQQDATACDTDMPQHATTQQDNDYKKNNELNIVENVAVPQQETTAYHSQTLQH
                     ATLQNVAVPQPKKRGLFGRVLNAVFNEE"
                     /name="DNA replication protein"
     gene            complement(10184..10957)
                     /locus_tag="SG90_019790"
     CDS             complement(10184..10957)
                     /locus_tag="SG90_019790"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_004942631.1"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="ANB90650.1"
                     /translation="MNILEETIKLGGKSIFFKRWIVPASHKTPIILLHESLGSVELWKS
                     FPQRLAEFLQREVIAYDRLGFGNSSPLEKLSLNFITEEAETTFAAVIQHLEIDQFIVMG
                     HSVGGGMTAVIAALYPDRCKAAITISAQSMVEEITRQGIKDAEANFCNEQFIKKLAKFH
                     GDKAEWVLRAWIDTWLSPAFRNWNIDAYISKIKSPLLVIHGENDEYATLIQPERLIQLS
                     NGYKELEILKDCGHFPHKEKETEVLTTIQSFLEKI"
                     /name="alpha/beta hydrolase"
     gene            11267..11554
                     /locus_tag="SG90_019795"
     CDS             11267..11554
                     /locus_tag="SG90_019795"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ANB90651.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="hypothetical protein"
     gene            11541..11855
                     /locus_tag="SG90_019800"
     CDS             11541..11855
                     /locus_tag="SG90_019800"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, antitoxin component"
                     /protein_id="ANB90652.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="toxin-antitoxin system, antitoxin component"
     gene            complement(11950..12384)
                     /locus_tag="SG90_019805"
     CDS             complement(11950..12384)
                     /locus_tag="SG90_019805"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_000780882.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="ANB90653.1"
                     /translation="MKLYISALQLENGELLLVVSPQFNANAIQDYALRWEIETLFSCLK
                     GRGFNLENTRLTDPRRVKKLIAVLAISFCWCYLTGEWQHDQKKAIKIKKHGRLSMSLFR
                     YGLDYVQMAIQRLIGFGKKEEFKEILAILRRQNPDRIRVL"
                     /name="transposase"
     gene            complement(12471..13040)
                     /locus_tag="SG90_019810"
     CDS             complement(12471..13040)
                     /locus_tag="SG90_019810"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_002037748.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="ANB90654.1"
                     /translation="MTHLNELYLILNKYLKWNKSHLKCFALIMLVIILKQTCNLSSASK
                     ALPIKCLPQSFYRRMQRFFAGQYFDYRQISQLIFNMFSFDQVQLTLDRTNWKWGKRNIN
                     ILMLAIVYRGIAIPILWTLLNKRGNSDTKERIALIQRFIAIFGKDRIVNVFADREFIGE
                     QWFTWLIEQDINFCIRVKKTSLSPII"
                     /name="transposase"
     gene            13126..13956
                     /locus_tag="SG90_019815"
     CDS             13126..13956
                     /locus_tag="SG90_019815"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_004647119.1"
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="ANB90655.1"
                     /translation="MKTLILLPLLSCLSLTACSLPVSNSSSQITSTQSIQTIAKLFDQA
                     QSSGVLVIQRGPHLQVYGNDLSRAHTEYIPASTFKILNALIGLQHGKATTNEIFKWDGK
                     KRSFAAWEKDMTLGQAMQASAVPVYQELARRIGLELMQQEVQRIRFGNQQIGQHIDNFW
                     LVGPLKITPEQEVEFASALAQEQLAFDPQVQQQVKAMLLLQERQGYRLYAKSGWGMDVE
                     PQVGWLTGWIETPQDEIVAFSLNMQMQSNMDPAIRLKILQQALAELALYPKAEG"
                     /name="class D beta-lactamase"
     gene            14090..14659
                     /locus_tag="SG90_019820"
     CDS             14090..14659
                     /locus_tag="SG90_019820"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_002037748.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="ANB90656.1"
                     /translation="MTHLNELYLILNKYLKWNKSHLKCFALIMLVIILKQTCNLSSASK
                     ALPIKCLPQSFYRRMQRFFAGQYFDYRQISQLIFNMFSFDQVQLTLDRTNWKWGKRNIN
                     ILMLAIVYRGIAIPILWTLLNKRGNSDTKERIALIQRFIAIFGKDRIVNVFADREFIGE
                     QWFTWLIEQDINFCIRVKKTSLSPII"
                     /name="transposase"
     gene            14746..15180
                     /locus_tag="SG90_019825"
     CDS             14746..15180
                     /locus_tag="SG90_019825"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_000780882.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="ANB90657.1"
                     /translation="MKLYISALQLENGELLLVVSPQFNANAIQDYALRWEIETLFSCLK
                     GRGFNLENTRLTDPRRVKKLIAVLAISFCWCYLTGEWQHDQKKAIKIKKHGRLSMSLFR
                     YGLDYVQMAIQRLIGFGKKEEFKEILAILRRQNPDRIRVL"
                     /name="transposase"
     ARG             13127..13953
                     /name="blaOXA-237"
                     /gene="blaOXA-237"
                     /gene_name="OXA-134 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-237"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          8319..9268
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.266"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtagatttta taaattttta agtgttatat tataatataa cattaaattt ggttgtttta
       61 tgctaaataa aagtaaacta tttttagctt taattacttt aggggcctcg aaaattttat
      121 tggcagctga agggcctgtt actaccttaa atacaatagt attaactgca caatctgatg
      181 agttagggtc tgagctgtta ggcaaaagtc taaatgttag taatcagttt attgatacta
      241 gtaaacttaa acagcgttcg acaactctag gggatgcctt gggtacggaa ttaggaattc
      301 attctaatca atatggaggt ggagcatcta ctcctattat tcgtgggcag gaaggaaaac
      361 gtattaaagt tttacagaat aatgctgacg tgttggatat gtctaatatg tctccagatc
      421 acgctgtcac agttgaacca agtttagcta agtctattga aattatacgt ggtgcaagta
      481 cccttttgta tagttcaaat agtgcggcag gggtagtcaa tgtcattgac tataaaattc
      541 caacccaaat gcctcaagac ggattagagg gtaatactac attacgtttt aatactggca
      601 gtaatgagaa gttgaccaca gcaggtgtaa cagttggctt aagcccacat gtagctttac
      661 gggcagaagg gttatatcgt aatgctggta actataaaac tcctcattac cagtctagct
      721 cttataattc tttagaagat cttgaaaacc aaaacagtat ttataaaaat ttaaaatatt
      781 taccagaaag ctgggcagag tcgagagtag gtacgcttgg attatcctgg atagacgata
      841 atacttattt aggagtttct tatacacatc gtcatgatga atatggttta ccagcccata
      901 gccatttata tgaaggttgt ggtgcctctg caatcggtat cgattcacga attagtggat
      961 taaaaaatta tcttttatat tatcctcaat tgatggatga gcaagatatt aattacataa
     1021 atccacgtcc tgattgtcat cagcataatc atattcatga aactaatttc tctcataatg
     1081 ctccttatat tgatttaaat actcgacgtt atgatgtgcg tggagaattc actcaaccat
     1141 ttaccggtat tgacaaaatt cgtacctcat tgagttatat agattatttt cacaatgaac
     1201 tcgagggaga taagattact aactttttta aaaatacagg aaaagtaggc cgcatagagt
     1261 tatcacatca gccattaggg gaattaaccg gtatcttggg gctacaatat ttggaacagg
     1321 ataatagtgc tttatcacca gtccattctc aagaaggcca taccacctat ctagataatc
     1381 agcagctatt aaatcgaaat gtcactaaaa actttagtgt atttggccta gaaaaatata
     1441 actggaacga ctttactttc gaactaggtg ctcgtataga aaagcagaaa gttagtatgg
     1501 attatgatat agaaaaaatt aaagattcaa tgaaaccttg gcctaataaa tataacagtc
     1561 cttatgtaga aaaaaataat aaaatcaggg ctcaaaattt aaaatctatt ttagaagctg
     1621 ttcaacctaa caaagaaaca gctttttctt atgcaggtac agtccactgg agatttgctc
     1681 caaattatat cttatcatta acaggtacac atcaagaaag attacctaat gcacaagaaa
     1741 tgtatactca tggtatgcat ctcgctacta attcttttga aataggtaac cgctttctaa
     1801 gaaaagagaa atccaataat ttagaaatca gtctggccta taaggatgat ttacttgatt
     1861 atcaaatcag tacttactat tatgattttg ataactatat atatttacag acactcaatg
     1921 aagttttagg aactacaaaa gttagagatc agcatacact acgtattaat cattatagcc
     1981 agtctgctgc taatttttat ggattagagg gcaacatagg gtatcaattt aattctgttt
     2041 atcacggctc tctttttgga gattatgtga aaggccgttt aacaaattta cctgatgctg
     2101 taattgcata cgatatctgg aatagagaac cgactttagc tccccaaaag gatcgttaca
     2161 ctcctagatt acctcctgct cgtttgggta cacgactaaa agctgatttt gatgaatcgc
     2221 ttaaaggtga aatagaatat tatcgagtct ttaaacaaga taatatttct aaatttgagc
     2281 aggttacatc aggttataat atgcttaata tgactttagc atataaaaat aagctatctc
     2341 acacagaata tgatttattc tttaaagcga ataatttact tgatcagaaa gtctatgccc
     2401 atgaaacatt tttgccatat attccccaaa ttggaagaaa ttttagttta ggcttaaatc
     2461 ttaatttcta gataagatac tgtaatcttc taagaaaatt tcttagaaga tttttcttat
     2521 aaaatgatta gattaataat taaggtaagc gtccgctgaa tcccatacct attttttaat
     2581 tcgatttagg tttccagcag tgtggcagta actcttcaat ctgggtcact ttatgtgtcg
     2641 gcagcctttt cagcactgaa tagccaccga ttttgtagac accttttagc tagataaaaa
     2701 gcaatttttt tgatctttaa agaatgagga tataagctgt ttaaaactta attctaattg
     2761 gttaataggt gaaaaatgaa attagcagaa gctctcttac ttcgaagtga tcaacaaaaa
     2821 aaaattattt cactaaaaca acgtatcaat gcaaatgtat tggtacaaga tggtgatcaa
     2881 ccatctgaag atccaaatga actactaaaa caagtatttt ctttaattca agaatttcaa
     2941 aaattaagtt atgcaattca tgaaactaat gctttaacaa aattaaatga tggccgttct
     3001 ctacttgctt tattgacttt gcgtgatgaa tttgtagaac agcacaaaac cctaactgca
     3061 gcaataagta atacatctcg tgaatctgat cgttatagta cgcgggaaat aaagtggcat
     3121 aaagttattc cagtttcatc attacaaaaa caagctgatg atattagtct taaattacgt
     3181 gatttaaacg tgttaattca gtcgactaat tggaaaattg atttgattga agcttaatag
     3241 tcctttagat ataatcaagt tgaacatact gggcgagtac aagatccctt acttccacaa
     3301 cataggattg taaataaagt gtagtggctt gcaaagcaag tggataagct taaaatttta
     3361 agtacaaatc aatccatcac ttggcataac tcaaatcttt gtgcaaacaa agttacacgt
     3421 tacttataac taccatgtac tgacagtatg ttcacctcaa ttactaaaaa taaagaaatg
     3481 agcaagcatc agctcaacta catttctatt tttaattcag ttcggcattt aacataaaat
     3541 ctcttatacg aaatctttta ctcaccaaaa attttattaa aatttccgta atcagttttt
     3601 tctttcagtt tatttaaaag ataagccgtt tgctccgtat ctaaatgccc ttcatcaatc
     3661 aatttgattg aatcagttaa tccgatgttc aaataacttg aatgaatact gacttcctta
     3721 gagttatttc ccaaaatgcc gctgagtaaa cttttaggtt gttgttcgag ttgctctaaa
     3781 gcgtagtttt caaaaacgtc tacgccccag atttcagtat agaagtctct tttattgaca
     3841 tgctcacata caaaagccac tttaatgtgt gaagctccaa caccataaac atgtttctgt
     3901 agtaaaaaat ttgctagacc agccttcgtc tcatccatca aattaaacaa agatgtacct
     3961 gttaattcag gataaaaaac caccaaagtt aattgatgtc ccttttcaaa cccctcttgc
     4021 acgaagccat aaaagatttt tattggatga ttgtcgctac cctcacttgg gtaataggct
     4081 tcatataaca ctttttctag tactcctata ttttcgtgaa ccgtactaag gtttttcgtt
     4141 attaaaaagt tttgagtttt aagcgacttt aaagtcccta attgttttta aattagtgcg
     4201 tttgggcatg ttgtagcact catagtattt cggcatttcc aatgttccgt aagagccgtt
     4261 atacatcaca acagccgtac agatcggcaa cttaataaat tcggcaggct caataataga
     4321 ttccaattta atggacttgg ttgtacctgt attttgcgac ctattggaag atgaactgct
     4381 tgcccgactc gatgaacttg taccgctcgt ctggcttgta cctgccgttt cagaaatata
     4441 tgtcgtttca gtttgaccga ctaatcgact gcattcctca gccgactcaa tgtccccaat
     4501 tctaaaaaca atttttaaat tgctagaccc catgaacatc ttgtcgctat ttgctccata
     4561 caagcgattt aagccctcta aactttgcag atagaggaat gtaggcatat ttgcagaacg
     4621 tatcgtattg agcaaatcag tgaattttgg gataggggca caattaataa tttcatctaa
     4681 ggcaatgaat accgcctcac gattttgatt tgcctgcaaa atacgcatgg tatgcgccac
     4741 catcgcccca aataatgtag ctgttgaact cttatactgt tggtcaaact gcagaaatac
     4801 ggcttgtttt ggcttcatta atacttcttt taaatagaac ccttgggtat tgtctgatgt
     4861 agttactcga atacgatcat catcaagaaa ctcaaatgct tttgaaacag ccgtcatcac
     4921 agatgcaagc aagcgatcat tttgggctgt tcgagaaact tctttagcaa tatttgaaac
     4981 actggatttt tcagactgtc ttaatgcctt taacagctca tcattactat ccatactgtt
     5041 aatataattt tgagcattca ccagtgatgc cttatcacca agttcgatga gaaccgcctt
     5101 aagaatacgt ctagcaaggt cattaaaaac cactgaatcg gctgtatcat ctttgatgat
     5161 aatgtttaaa atctcattga gttctgctga atcctttact tctgaaaaaa tattgtagtg
     5221 gtcactatcc acatccgttg gattaaaaac ccaatcggta taaccgaaaa ttttaaagac
     5281 attgtttgct ttgagaatcg cccaaatttc tggctttacg tctgttgcaa cgaagctacg
     5341 acccgacttc atccactcca cgatttgaga gattaaaaac gttgttttac ctgaaccagg
     5401 tgcgcctaac acaatgcccc gatcttcatt tgtcaccctt agactttcac gatggaacga
     5461 ccaatcaaga tatttttgtt ccatctcttt aatttgcttt ttgggtaaag tctgatccgt
     5521 aaaggcaatt aatggcgttt tattttcaaa tccacgcttt aattcgctta acaatgtccc
     5581 atagccttcg gcagactctt tttttattcg ttcaacacgg ggttttgact tcttcccagt
     5641 aaaatacaga taaacatata acccaatgat tacagcccat gaaaagaagt aatttttttc
     5701 tggtacaaca acataattat ttggaagtaa gttggtgata taactcaata gataggtatg
     5761 cgctaaaacg actacagcaa gcagaacaat gttatagccg atctcatata ctggatcatg
     5821 tgctttataa attttcttta taccgacaac aatcatcgta aaaattacaa taattgctaa
     5881 agcaaacagc gtatttgcaa aaccaacaaa cagcggttta aggtgcaatc catttattaa
     5941 atagccgtaa tagctcataa aaaaattatg tagcatttag tcactccctt tctgctgctg
     6001 tttaccttca caggtatcta gaaattgagg ttcagataca ggacttttga gccatgaaca
     6061 acgaacggta taataaaaag atgcttctga gcttctaaat ttaattaaat tttgagtaga
     6121 ttttagttcc gctttatcat tccaaatcaa tagcttgcaa ttaatacaac gaccacacaa
     6181 cggcaaactt gatcttattt ctgatgcaat acccatctaa tttttactcc atgaacccaa
     6241 gtacacggac gtaatattga ttcgactatg tcccaattct tcactaatta acatacggat
     6301 ttctttatct tttacttgct cgtcagccga caatgaacga gaaccgccta cagcagggca
     6361 atcgaaaccc attaattcac ggtatctggt ctgagcgtag ccatgtcgta aaccatgcgt
     6421 ttgtcccaca cctagcgcat gcgtgactgc ttcaaaattg gctttgtggg acttatagct
     6481 tttttctttc ggaatcattg aaccgccatt tttcacagct tgagcgtagg catttttgag
     6541 ttcttgacgt tgtttttcgt tggttattgg aatagttcga ggtcttccgc ctttcgtcca
     6601 actgtctttt aaacggatat atttagcatt ttcaattttc tgaccatcta aagcatattc
     6661 aggttggaac ttcatgcttt cttcacgtct aagcccgaaa gcatcttgta aacgtaagct
     6721 ttgcgctatg tttggatcag ttttagacaa atctaaatct tttaaagaaa tagatttatt
     6781 ttcattggtc acatagacac gattctctat tttataagtt gcattgtctt tgacagcacc
     6841 actattacca actttttcag cccaccacct tacagccacc atccgatttt ttattgttcc
     6901 agtggacaca ccatctttac gccattgctc aagcaattta tttacatgcc gaccttttaa
     6961 atctttaggc gacatggttt taacatcata accattcttg gcaagctgtt cagtaaacag
     7021 tttcagcata gctcgcctat ttgcttgtgt ggcgtgtgaa ccttcacgga acttattgca
     7081 gagctgatca aattgatact ctaaggtcat gtgtcctctt ttattcgaga tgctttattg
     7141 atgagtacag cacagcttta tgctgggcta cggaatcaat gaagcatcat ttttttaatt
     7201 tataactatt tgagatccga tgtactggtt agtgttaaga gccgaattaa cgttttatca
     7261 aagtcaatta tagaaattgg ttcgataaaa aatgctcaaa agtgggtata aaaattcgag
     7321 aatctttaaa tccaaatata tgaatactta taaaaataaa ccgtttctgc atataggatg
     7381 agagcgatta aaagtatttg atctccaata ttaagtttgt gtttaagctg aaaaacataa
     7441 tgaaagtcag caaagaaaag ttatgaaaaa acaagaaagt ttgatcatta tatcgttgat
     7501 ttagttactg tcacgcatct ataactgaat atttcgtgtt caattgtaac tgttataatc
     7561 tttttcccag tcgtcactac tgataagtag tgacgactgg ggagcatggc cactgtttac
     7621 gcatatcaaa aagggcggtc gccacccgtt cgggatggct ctgctactcc ctgaaaaatt
     7681 cagatgggag ctggtagtca gagggaataa gttttgagat ttagattttt tctggtcgct
     7741 cacactccgt tcgctttgta cttttctttt tgacttaaac aggtttaaac cgagcgagtg
     7801 tctacgagcg attgagcatt ataaaaattt cgcccctgcc ttttccctga ttacaagctc
     7861 cctttcgatt ttaaacggta gatacagagc atcccgaatg ggtgcgaaca aacttaattt
     7921 gaatctagac cgagcgaagc gagtaaaaaa gcttatgagc gaagcgaatt ccgagttgct
     7981 tttgcttttt cttaaagtca cgaaaatatt aaccaaaaaa ttacccctcg aatcgagcga
     8041 aagcgagatt caatagagtt tgagcgaagc gaaaaccaag ggtaattttt cattccctgt
     8101 gcttttaatt attttaaatt ttttaaatgc ttttagatgg cttgaaagcc ttatgaataa
     8161 agggtttcat ctcttatatg tccacgttta ccttgcaata tgtccacgtt taccttgcaa
     8221 tatgtccacg tttaccttgc aatatgtcca cgtttacctt gcattagtac acaaataata
     8281 ttaacgtgta tttatacaca ataaaaaaat agtggctatg agagatttag ttgtaaaaga
     8341 caatgcctta atcaacgcaa gctataactt agacttagta gaacaacgtt taattttatt
     8401 ggctattgtt gaagcaaggg aaagtgggaa agggattaat gcaaatgatc cattagaagt
     8461 tcatgcagat agttatatca atcaatttgg tgtacaccgt aatacagctt atcaagcctt
     8521 aaaagatgct tgtaaggatt tattcgcgcg tcaatttagc tatcaagaga aaaaagctaa
     8581 tgggaatatc cgaaatgtta tgagtcgttg ggtatctcaa attgcttata acgacaatga
     8641 agcaactgta gacttaatat ttgcacctgc tgttgttccc ttcataacca gactggaaga
     8701 acaatttact aaatatgaat tacagcaagt tagtagtctt agtagcgctt atgccattcg
     8761 cttatatgag cttctaattc agtggcgaag cactggtaaa accccaacta tagaactaca
     8821 agaatttaga aagaagttag gcgttcttga taatgaatat ttacggatgg ctcatttaaa
     8881 agagcgtgtt ttagagcttt caattaaaca aataaatgag catacggata taactgtaaa
     8941 atatgaacag cataaaagag gacgttctat ttcaggattt tcttttacct ttaaacagaa
     9001 aaagaaggat agcccatcaa tagaaagaga tccgaatacc ttagaccttt tttcaaagat
     9061 gaccgatgct caacggcata tgtttgcaaa caaactttca gaactccctg aaatgggtcg
     9121 ctattcacaa ggaactgaaa gctatcctca atttgctgtt cgtattgctg agatgctaca
     9181 agactctgaa aaaatcaaag aactagcccc atacctaaaa aaagtgggat acatgccatc
     9241 aaataaaaag gacacagtaa atggctaagt tatcgctaag tgaagtatct aaaaaatttc
     9301 atattgatag gtcaaccatt tatagagctg tacgtaatgg gcgtttatca cgctctagtg
     9361 atggtcaatt tgacctttcg gaagtgatta gatgctttgg ggagcctgag caaatatctc
     9421 aacaaattga accatctaaa tcagataatg atgaatctac aaaaaaactc attattcatt
     9481 tagaaaatga agtcaaaaaa taccaagaac gtgaagagcg tttaatgcag caaatcgacc
     9541 gtatgcagac gcttattgag ttaaaaactg ttgcacccgc cacggcagcg ccacaacaag
     9601 atgctacggc atgcgacacc gatatgccac agcatgcgac aacacaacaa gacaatgatt
     9661 ataaaaagaa taatgaatta aatattgtag aaaatgtggc tgtgccacag caagaaacta
     9721 cagcatacca cagccaaacg ctacagcatg ccacattaca aaatgtggca gtgccacaac
     9781 ctaaaaaacg aggcttattt ggccgagtac tgaatgcggt attcaatgaa gagtaagctc
     9841 tccaaggagc attgtttatg aaaaaactga aagagatcct gcaaggtatt gcctttttat
     9901 ttgttgggat tccgatcatg atcatggcca gcatcataga tcaacgtgcc ttcaaaaaag
     9961 agatggatga acgccaaaaa gaaaaagacc aggcatcctg attattcaat tgagctaatc
    10021 aaagtcgtga aacattggtc aaagaatagg cgtggaacgt gaaaaatcac aaaaaagccg
    10081 ttctagtgcg ctgaacccta gaacggctta ggctaataca ttgatgctaa aaggaaaaat
    10141 ccaagcattt cgtataacgt gtattatgtt aattttagaa aaactaaatt ttctctaaga
    10201 aactttggat agttgttagt acctctgttt ccttttcttt gtgagggaaa tgaccacaat
    10261 cttttagaat ttctagctct ttatatccat tagaaagctg aataaggcgt tctggttgaa
    10321 tcaaagttgc atattcatca ttttccccat gtattactaa aagaggactt tttattttcg
    10381 atatataggc atctatattc caattacgga atgctggtga taaccaagta tcgatccatg
    10441 ctcttaatac ccattcagct ttatctccat gaaacttagc taatttctta atgaattgtt
    10501 cattacaaaa attcgcttcc gcatctttta taccctgtcg agtaatttct tcaaccatag
    10561 actgtgcgga tatagtaatt gctgctttgc aacgatctgg atataaagca gctattacag
    10621 cagtcatccc accaccaaca ctatggccca taacaataaa ttgatctatt tctagatgtt
    10681 gtattacagc agcaaaagtt gtttctgctt cctcagtaat aaaattaaga gataattttt
    10741 ctaagggtga ggagtttcca aatcctaagc gatcataagc aataacttct ctctggagaa
    10801 attcagctaa acgttgagga aagcttttcc aaagctcaac gcttcctaaa gattcatgaa
    10861 gcaaaataat gggagtttta tgagaagcag ggacaatcca tcgtttaaaa aatatagatt
    10921 taccacccaa ttttatagtt tcttctagaa tattcatttt gtattcagct taataaagat
    10981 gctgttattc taatgaaaag tagagcatct atactgaccg tagagtcgca catttaacat
    11041 aatgggcgtt atacgaagca cacttgtata ttaatttaaa aatcaattac ttaataaaaa
    11101 tagaaaaatc caaaaatgcc caaaaagccg actttgaaac aagtcggctt ttttatgagc
    11161 agttttgata cttcttgcca ataattttga ctaaaaatta cacgaatctg gttgagtgat
    11221 acaattttgt gatacattat tgtatataca aatttgaatg ttttttatgg aacagtattt
    11281 tgaatgggat gaggctaaga atcgaaagaa tcagaaaaag catgatatct ctttcgaaac
    11341 tgcaagcctt gtttttgaag atccattacg gatctcaatc caagacagac ataccaatgg
    11401 tgaagaacgt tggcaaacca ttggacgagt taaaggcgta ctaatgttat tggtggctca
    11461 caccattttt gatgaagatg actgtgaaat catccgaatc attagtgcaa ggcaagtcac
    11521 taaagcggag cgaaacaaat atgagcatgg ttagatactc acacaaagag ctgaatgaga
    11581 aatttggtga gaagcaagat gctgaaattc aacgcttgct tgctaaaggc acggttcctg
    11641 atgatcagct agatctatca gatattcctg aaattacaga ctggagcaat gctgtacgcc
    11701 aaaatcaatt ttaccgccca gtaaagcaac aaacttcaat tcggttagat gctgatgtac
    11761 ttgcttggtt caaagcacaa ggcaaaggtt atcaaacacg tatgaatgaa attttacgag
    11821 atgctatgct aaaagaatta aaaaatcatc aataagaatg ggagcttagg ctcccatttt
    11881 tgacttcatt tttaggggga ttaccattca aaaatttaaa tagtagattt tctctgtaca
    11941 cgacaaattt cacagaaccc ttatcctatc agggttctgc cttcttaaaa ttgccaaaat
    12001 ttccttaaac tcttcttttt tcccaaaacc aattaaacgc tgaatcgcca tttgaacata
    12061 gtctaaacca tagcgaaata aactcattga gagtcgtcca tgcttcttta tttttatcgc
    12121 ttttttttga tcatgttgcc attcacccgt taagtaacac caacagaagc ttatagctaa
    12181 caccgcaatc aattttttca ctcgtctagg gtctgtcaag cgcgtatttt caagattaaa
    12241 cccgcgtcct ttgagacaac tgaataaggt ttcaatttcc cagcgtaatg cataatcctg
    12301 aatagcattg gcattaaact gaggagaaac gacgagtaaa agctctccat tttctaactg
    12361 tagtgcactt atatatagtt tcacccgacc aaccaaaatc cgtcgtttac gacattcaat
    12421 ttgaccaact tgaagatggc gaaataaatc actaatttta tgattctttc ctaaatgatt
    12481 ggtgacaatg aagttttttt aacacgaatg cagaagttga tgtcttgttc aattaaccat
    12541 gtaaaccact gctcaccgat aaactctctg tctgcgaaca cattcacaat acggtcttta
    12601 ccaaaaatgg ctataaagcg ttgaatcaaa gcaatacgct ctttcgtatc tgaatttcca
    12661 cgtttattaa gcaatgtcca aaggataggt atcgctattc cacgataaac gattgcgagc
    12721 atcaggatat taatatttcg ttttccccat ttccaattgg ttctatctaa agtcagttgc
    12781 acttggtcga atgaaaacat attgaaaatc aactgagaaa tttgacgata atcaaaatac
    12841 tgacctgcaa agaagcgctg catacgtcga taaaatgatt gtggtaagca cttgatgggc
    12901 aaggctttag atgcagaaga aagattacat gtttgcttta aaataatcac aagcatgatg
    12961 agcgcaaagc actttaaatg tgacttgttc cattttagat atttgtttaa gataagatat
    13021 aactcattga gatgtgtcat agtattcgtc gttagaaaac aattattatg acattatttc
    13081 aatgagttat ctatttttgt cgtgtacaga gaatatcctg aacttatgaa aactcttatt
    13141 ttgttgcctt tacttagttg cttgagcctg acagcctgta gcttgcctgt ttcaaattcg
    13201 tcctctcaaa tcacttcaac tcaatctatt caaaccattg ccaaattatt tgatcaggca
    13261 caaagctctg gcgttttagt aattcaacgg ggcccacatc tacaggtcta tggcaatgat
    13321 ttgagtcgtg cacataccga atatattcct gcttcaacct ttaaaatact caatgccctg
    13381 attggcctgc aacatggtaa agccacgacc aatgaaatct ttaaatggga tggcaagaag
    13441 cgcagttttg cagcctggga aaaagacatg actctcggcc aagccatgca agcttctgct
    13501 gtacccgtct atcaggaact ggcacgtcgc attggtctgg aactaatgca acaggaagtg
    13561 caacgcattc gatttggtaa tcagcagatt ggtcagcata tcgacaactt ctggttagtc
    13621 ggacctttga aaatcacccc ggaacaagaa gtcgaatttg cctctgcgct tgctcaagag
    13681 caacttgcct ttgatcctca agtccagcaa caagtcaaag ccatgttact gttacaggag
    13741 cgacaaggtt atcgactata tgccaaatct ggttggggta tggatgtgga gccgcaagtc
    13801 ggctggctca ccggctggat cgaaacacct caggacgaaa tcgtggcatt ttcactgaat
    13861 atgcagatgc aaagtaatat ggatccggcg atccgtctta aaattttgca gcaggccttg
    13921 gccgaattag cgctttatcc gaaagctgaa gggtaacgta aataggcatg ggaaaaacgc
    13981 caaatttaag ctatattttt aagaataaac atttaaatct ctgtacacga caaaaataga
    14041 taactcattg aaataatgtc ataataattg ttttctaacg acgaatacta tgacacatct
    14101 caatgagtta tatcttatct taaacaaata tctaaaatgg aacaagtcac atttaaagtg
    14161 ctttgcgctc atcatgcttg tgattatttt aaagcaaaca tgtaatcttt cttctgcatc
    14221 taaagccttg cccatcaagt gcttaccaca atcattttat cgacgtatgc agcgcttctt
    14281 tgcaggtcag tattttgatt atcgtcaaat ttctcagttg attttcaata tgttttcatt
    14341 cgaccaagtg caactgactt tagatagaac caattggaaa tggggaaaac gaaatattaa
    14401 tatcctgatg ctcgcaatcg tttatcgtgg aatagcgata cctatccttt ggacattgct
    14461 taataaacgt ggaaattcag atacgaaaga gcgtattgct ttgattcaac gctttatagc
    14521 catttttggt aaagaccgta ttgtgaatgt gttcgcagac agagagttta tcggtgagca
    14581 gtggtttaca tggttaattg aacaagacat caacttctgc attcgtgtta aaaaaacttc
    14641 attgtcacca atcatttagg aaagaatcat aaaattagtg atttatttcg ccatcttaaa
    14701 gttggtcaaa ttgaatgtcg taaacgacgg attttggttg gtcgggtgaa actatatata
    14761 agtgcactac agttagaaaa tggagagctt ttactcgtcg tttctcctca gtttaatgcc
    14821 aatgctattc aggattatgc attacgctgg gaaattgaaa ccttattcag ttgtctcaaa
    14881 ggacgcgggt ttaatcttga aaatacgcgc ttgacagacc ctagacgagt gaaaaaattg
    14941 attgcggtgt tagctataag cttctgttgg tgttacttaa cgggtgaatg gcaacatgat
    15001 caaaaaaaag cgataaaaat aaagaagcat ggacgactct caatgagttt atttcgctat
    15061 ggtttagact atgttcaaat ggcgattcag cgtttaattg gttttgggaa aaaagaagag
    15121 tttaaggaaa ttttggcaat tttaagaagg cagaaccctg ataggataag ggttctgtga
    15181 aatttgtcgt gtacagag
//
