LOCUS       CP011993               13841 bp    DNA     circular BCT 20-AUG-2015
DEFINITION  Klebsiella pneumoniae UHKPC33 plasmid pUHKPC33-13.841kb, complete
            sequence.
ACCESSION   CP011993
VERSION     CP011993.1
DBLINK      BioProject: PRJNA187294
            BioSample: SAMN02142014
KEYWORDS    .
SOURCE      Klebsiella pneumoniae UHKPC33
  ORGANISM  Klebsiella pneumoniae UHKPC33
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 13841)
  AUTHORS   Wright,M.S., Perez,F., Brinkac,L., Jacobs,M.R., Kaye,K., Cober,E.,
            van Duin,D., Marshall,S.H., Hujer,A.M., Rudin,S.D., Hujer,K.M.,
            Bonomo,R.A. and Adams,M.D.
  TITLE     Population structure of KPC-producing Klebsiella pneumoniae isolates
            from midwestern U.S. hospitals
  JOURNAL   Antimicrob. Agents Chemother. 58 (8), 4961-4965 (2014)
   PUBMED   24913165
REFERENCE   2  (bases 1 to 13841)
  AUTHORS   Brinkac,L.M., Durkin,A.S., Haft,D.H., McCorrison,J., Wright,M.,
            Duin,D.V., Kaye,K.S., Marshall,S., Rudin,S., Hujer,A., Perez,F.,
            Bonomo,R.A. and Adams,M.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2013) The J. Craig Venter Institute, 9704 Medical
            Center Dr., Rockville, MD 20850, USA
REFERENCE   3  (bases 1 to 13841)
  AUTHORS   Adams,M., Sanka,R., Wright,M., Brinkac,L., Perez,F., Jacobs,M.,
            Kaye,K., Cober,E., Duin,D., Marshall,S., Hujer,A., Rudin,S.,
            Hujer,K. and Bonomo,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2015) JCVI, 9704 Medical Center Drive, Rockville,
            MD 20850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: HGAP v. 3
            Assembly Name                :: Klebsiella pneumoniae UHKPC33
            Expected Final Version       :: Yes
            Genome Coverage              :: 3.93x
            Sequencing Technology        :: PacBio RS
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 07/01/2015 10:18:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision :: 2.10
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes                        :: 5,757
            CDS                          :: 5,585
            Pseudo Genes                 :: 55
            rRNAs                        :: 25 (5S, 16S, 23S)
            tRNAs                        :: 87
            ncRNA                        :: 5
            Frameshifted Genes           :: 41
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This strain is part of the Klebsiella pneumoniae Genome Sequencing
            Center Project as part of the Genome Sequencing Centers for
            Infectious Diseases contract.
            http://gsc.jcvi.org/projects/gsc/klebsiella_pneumoniae/index.php.
            Source DNA and/or bacteria are available from the laboratory of Dr.
            Robert Bonomo, Cleveland VA Medical Center, 19701 East Boulevard,
            Cleveland, OH 44106, USA.  This project has been funded in whole or
            in part with federal funds from the National Institute of Allergy
            and Infectious Diseases, National Institutes of Health, Department
            of Health and Human Services through the Genomic Sequencing Centers
            for Infectious Diseases under contract number HHSN272200900007C.
            This genome has a base modification file available.
FEATURES             Location/Qualifiers
     source          1..13841
                     /organism="Klebsiella pneumoniae UHKPC33"
                     /mol_type="genomic DNA"
                     /strain="UHKPC33"
                     /db_xref="taxon:1284802"
                     /plasmid="pUHKPC33-13.841kb"
     gene            complement(join(13757..13841,1..737))
                     /locus_tag="H222_28710"
     CDS             complement(join(13757..13841,1..737))
                     /locus_tag="H222_28710"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_004199321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytotoxic family protein"
                     /protein_id="AKS03427.1"
                     /translation="MSFRSTERQTPTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDK
                     AKAELDAEDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEY
                     GSGWQDQVRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKD
                     AENKLNEEKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYG
                     DKKRKIYEWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cytotoxic family protein"
     gene            complement(1078..1782)
                     /locus_tag="H222_28715"
     CDS             complement(1078..1782)
                     /locus_tag="H222_28715"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_001067856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="AKS03412.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="transposase"
     gene            complement(1794..2063)
                     /locus_tag="H222_28720"
     CDS             complement(1794..2063)
                     /locus_tag="H222_28720"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_004153130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside resistance protein"
                     /protein_id="AKS03413.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETALLQIVGGDKLIIPFC"
                     /name="aminoglycoside resistance protein"
     gene            complement(2133..2738)
                     /locus_tag="H222_28725"
     CDS             complement(2133..2738)
                     /locus_tag="H222_28725"
                     /inference="EXISTENCE: similar to AA
                     sequence:SwissProt:P19650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase"
                     /protein_id="AKS03414.1"
                     /translation="MSIQHFQTKLGITKYSIVTNSNDSVTLRLMTEHDLAMLYEWLNRS
                     HIVEWWGGEEARPTLADVQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGW
                     WEEETDPGVRGIDQLLANASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAI
                     RCYEKAGFERQGTVTTPDGPAVYMVQTRQAFERTRSVA"
                     /name="aminoglycoside adenylyltransferase"
     gene            complement(2921..3478)
                     /locus_tag="H222_28730"
     CDS             complement(2921..3478)
                     /locus_tag="H222_28730"
                     /inference="EXISTENCE: similar to AA
                     sequence:SwissProt:P0ADI2.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="AKS03415.1"
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase"
     gene            3640..6645
                     /locus_tag="H222_28735"
     CDS             3640..6645
                     /locus_tag="H222_28735"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_001496645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="AKS03416.1"
                     /translation="MPVDFLTTEQVESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHDEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISQSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="transposase"
     gene            6722..6970
                     /locus_tag="H222_28740"
     CDS             6722..6970
                     /locus_tag="H222_28740"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_005144374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AKS03417.1"
                     /translation="MMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGT
                     TQFAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="hypothetical protein"
     gene            7276..7539
                     /locus_tag="H222_28745"
     CDS             7276..7539
                     /locus_tag="H222_28745"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="AKS03418.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            7529..7828
                     /locus_tag="H222_28750"
     CDS             7529..7828
                     /locus_tag="H222_28750"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin RelE"
                     /protein_id="AKS03419.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="addiction module toxin RelE"
     gene            complement(7890..8267)
                     /locus_tag="H222_28755"
     CDS             complement(7890..8267)
                     /locus_tag="H222_28755"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="AKS03420.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            8336..8515
                     /locus_tag="H222_28760"
     CDS             8336..8515
                     /locus_tag="H222_28760"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase"
                     /protein_id="AKS03421.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="RNA polymerase"
     gene            complement(8543..9073)
                     /locus_tag="H222_28765"
     CDS             complement(8543..9073)
                     /locus_tag="H222_28765"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_001240350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AKS03422.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(9080..9811)
                     /locus_tag="H222_28770"
     CDS             complement(9080..9811)
                     /locus_tag="H222_28770"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC"
                     /protein_id="AKS03423.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC"
     gene            complement(9811..11775)
                     /locus_tag="H222_28775"
     CDS             complement(9811..11775)
                     /locus_tag="H222_28775"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mobilization protein A"
                     /protein_id="AKS03424.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="Mobilization protein A"
     gene            complement(13256..13405)
                     /locus_tag="H222_28780"
     CDS             complement(13256..13405)
                     /locus_tag="H222_28780"
                     /inference="EXISTENCE: similar to AA
                     sequence:SwissProt:P02987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="AKS03425.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="lysis protein"
     gene            complement(13490..13747)
                     /locus_tag="H222_28785"
     CDS             complement(13490..13747)
                     /locus_tag="H222_28785"
                     /inference="EXISTENCE: similar to AA
                     sequence:SwissProt:P02986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="AKS03426.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     ARG             complement(2137..2687)
                     /name="aac(6')-Ib"
                     /gene="aac(6')-Ib"
                     /gene_name="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(1795..2051)
                     /name="aadA15"
                     /gene="aadA15"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.14"
                     /coverage="32.32"
     ARG             complement(2134..2738)
                     /name="AAC(6')-Ib10"
                     /gene="AAC(6')-Ib10"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="98.97"
                     /coverage="99.01"
     Typing          12575..13153
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9084..9811)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.34e-150"
ORIGIN
        1 ccatcactgg cgcggtaccc ttcaagctca ccgtgctggg aatcccattc ataaatttta
       61 cgctttttat caccatacca tcgggcccgc ttaccaccac caccttgttt aggggtttta
      121 ggtttaccct ctttcaactc tcccaacccc ttaatatctt cagtcttggg atcaggaaaa
      181 taatcatggc cgtaatcttt agttccctta cgaggttttt tcttttcctc atttaattta
      241 ttttctgcat ccttcgcttt tttctccttt tgtttccggc tctccatcgc cccggaaagc
      301 gcagccttat ccctggataa ggattcattc atttcgttta aagatgtctg ggcagctttt
      361 aatttctcat tctgattctg aatatcctta tcaagatagc gaacctgatc ctgccacccg
      421 gagccatatt catgaggcgg gttataagtc acaaaatcat ctttatttgc ctctgcctct
      481 tttacctttt tatcggcttc ctgaacagca gcccttgcac cggggatcgc cttctctgtc
      541 gatgcaatgc gactgtttaa ggtcgcaata tttttatctt cagcatccag ctcagctttc
      601 gctttatcat actctctttt cagcccctct tccgggtgag cagcgtccca tgcctgctgg
      661 cgacgctttt cttcctccag acgctgtttt acctgtgccg gagtaggggt ctgacgctca
      721 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      781 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
      841 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
      901 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
      961 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1021 atcagggcac tgttgcaaag ttagcgatga ggcagccttt tgtcttattc aaaggcctta
     1081 catttcaaaa actctgctta ccaggcgcat ttcgcccagg ggatcaccat aataaaatgc
     1141 tgaggcctgg cctttgcgta gtgcacgcat cacctcaata cctttgatgg tggcgtaagc
     1201 cgtcttcatg gatttaaatc ccagcgtggc gccgattatc cgtttcagtt tgccatgatc
     1261 gcattcaatc acgttgttcc ggtacttaat ctgtcggtgt tcaacgtcag acgggcaccg
     1321 gccttcgcgt ttgagcagag caagcgcgcg accataggcg ggcgctttat ccgtgttgat
     1381 gaatcgcggg atctgccact tcttcacgtt gttgaggatt ttacccagaa accggtatgc
     1441 agctttgctg ttacgacggg aggagagata aaaatcgaca gtgcggcccc ggctgtcgac
     1501 ggcccggtac agatacgccc agcggccatt gaccttcacg taggtttcat ccatgtgcca
     1561 cgggcaaaga tcggaagggt tacgccagta ccagcgcagc cgtttttcca tttcaggcgc
     1621 ataacgctga acccagcggt aaatcgtgga gtgatcgaca ttcactccgc gttcagccag
     1681 catctcctgc agctcacggt aactgatgcc gtatttgcag taccagcgta cggcccacag
     1741 aatgatgtca cgctgaaaat gccggccttt gaatgggttc atgtgcagct ccatcagcaa
     1801 aaggggatga taagtttatc accaccgact atttgcaaca gtgccgtttc caaaaggtcg
     1861 ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca
     1921 atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc
     1981 aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact
     2041 tcggcgatca ccgcttccct catgatgttt aacgtttgac atgaggggcg gccaagggcg
     2101 ccagcccttg gacgtccccc tcgatggaag ggttaggcaa cactgcgtgt tcgctcgaat
     2161 gcctggcgtg tttgaaccat gtacacggct ggaccatctg gggtggttac ggtaccttgc
     2221 ctctcaaacc ccgctttctc gtagcatcgg atcgctcgca agttgctcgg cgacgggtcc
     2281 gtttggatct tggtgacctc gggatcattg aacagcaact caaccagagc tcgaaccagc
     2341 ttggttccca agcctttgcc cagttgtgat gcattcgcca gtaactggtc tattccgcgt
     2401 actcctggat cggtttcttc ttcccaccat ccgtccccgc ttccaagagc aacgtacgac
     2461 tgggcatacc caatcggctc tccattcagc attgcaatgt atggagtgac ggactcttgc
     2521 gctaaaacgc ttggcaagta ctgttcctgt acgtcagcaa gtgtcgggcg tgcttcttct
     2581 ccgccccacc actcgacgat atgagatcga tttagccact catagagcat cgcaaggtca
     2641 tgctcagtca tgaggcgcag tgtgacggaa tcgttgctgt tggtcacgat gctgtacttt
     2701 gtgatgccta actttgtttg gaaatgttga atactcatac tcttcctttt tcaatattat
     2761 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2821 aataaacaaa taggggttcc gcgcactttt ccccgaaaag tgccacctga cgtctaagaa
     2881 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
     2941 caagaatttt ataaaccgtg gagcgggcaa tactgagctg atgagcaatt tccgttgcac
     3001 cagtgccctt ctgatgaagc gtcagcacga cgttcctgtc cacggtacgc ctgcggccaa
     3061 atttgattcc tttcagcttt gcttcctgtc ggccctcatt cgtgcgctct aggatcctcc
     3121 ggcgttcagc ctgtgccaca gccgacagga tggtgaccac catttgcccc atatcaccgt
     3181 cggtactgat cccgtcgtca ataaaccgaa ccgctacacc ctgagcatca aactctttta
     3241 tcagttggat catgtcggcg gtgtcgcggc caagacggtc gagcttcttc accagaatga
     3301 catcaccttc ctccaccttc atcctcagca aatccagccc ttcccgatct gttgaactgc
     3361 cggatgcctt gtcggtaaag atgcggttag cttttacccc tgcatctttg agcgctctga
     3421 tctgaatatc gagggactgc tggctggttg agacccgcgc ataaccaaaa attcgcataa
     3481 aaatgtacct taaatcgaat atcagacacg atgtgtctat tatgccaaaa tgacgattta
     3541 atggacactc aaacgaagcc gttttactat gtctgataat ttataatatt tcgaacggtt
     3601 gcagttgtgt taaaaaagcc gtcaggcagg gaggccgata tgcccgttga ttttttgacc
     3661 actgagcagg ttgagagtta tggcaggttc actggcgaac ccgatgaact tcagctggcg
     3721 cgttattttc atcttgatga agcggataaa gaatttatcg ggaaaagccg gggtgatcac
     3781 aatcgacttg gtattgccct gcaaatcggg tgtgtgcgtt ttctgggcac ttttcttact
     3841 gacatgaatc atattccttc cggcgtccgg cattttaccg ccagacagct cgggattcgt
     3901 gatatcaccg ttcttgcaga atacggtcag agggaaaata cccgccgtga gcatgcagcg
     3961 ctgatacgtc agcactatca gtatcgtgaa tttgcctggc cctggacatt tcgccttacc
     4021 cgtcttttat atacccggag ctggataagc aacgaacgtc ctggcctgct tttcgacctg
     4081 gcgacagggt ggcttatgca acatcgtatt attctccccg gagccaccac gctgacccgg
     4141 ttgatttcag aggtaaggga aaaggcgacg ttgcgcctgt ggaacaaact ggcactgata
     4201 ccgtcagccg aacagcgttc acagctggag atgctgctgg ggccaactga ttgcagccgc
     4261 ctgtctttac tggaatcact gaaaaaaggc cctgtgacca tcagtggtcc ggcgtttaat
     4321 gaagcaattg aacgctggaa aactctgaac gattttggcc tgcatgctga aaacctgagt
     4381 acactcccgg ctgtgcgcct gaaaaatctc gcacgttatg ctggtatgac ttcggtgttc
     4441 aatattgcca ggatgtcacc gcagaaaagg atggcggttc tggttgcctt tgtccttgca
     4501 tgggaaacgc tggcgctgga tgatgcactg gacgttctgg acgccatgct ggccgttatc
     4561 atccgtgacg ccagaaagat tgggcagaaa aaacggctcc gctcgctgaa ggatctggat
     4621 aaatctgcat tggcgctcgc cagcgcatgt tcgtacttgc tgaaagaaga aacaccggac
     4681 gaatcgattc gtgctgaggt gttcagctac atccctaggc aaaagctggc tgaaatcatc
     4741 acgcttgtcc gtgaaattgc ccggccctca gacgataatt ttcatgacga aatggtggag
     4801 cagtacgggc gcgttcgtcg tttcctgccc catctgctga ataccgttaa attttcatcc
     4861 gcacctgccg gggttaccac tctgaatgcc tgtgactacc tcagccggga gttcagctca
     4921 cggcggcagt tttttgacga cgcaccaacg gaaatcatca gtcagtcatg gaaacggctg
     4981 gtgattaaca aggaaaaaca tatcacccga aggggataca cgctctgctt tctcagtaaa
     5041 ctgcaggata gtctgagacg gagggatgtc tacgttaccg gcagtaaccg gtggggagat
     5101 cctcgtgcaa gattactaca gggtgctgac tggcaggcaa atcggattaa ggtttatcgt
     5161 tctttggggc acccgacaga cccgcaggaa gcaataaaat ctctgggcca tcagcttgat
     5221 agtcgttaca gacaggttgc tgcacgtctt ggcgaaaatg aggctgtcga actcgatgtt
     5281 tctggcccga agccccggtt gacaatttct cccctcgcca gtcttgatga gccggacagt
     5341 ctgaaacgac tgagcaaaat gatcagtgat ctgctccctc cggtggattt aacggagttg
     5401 ctgctcgaaa ttaacgccca taccggattt gctgatgagt ttttccatgc cagtgaagcc
     5461 agtgccagag ttgatgatct gcccgtcagc atcagcgccg tgctgatggc tgaagcctgc
     5521 aatatcggtc tggaaccact gatcagatca aatgttcctg cactgacccg acaccggctg
     5581 aactggacaa aagcgaacta tctgcgggct gaaactatca ccagcgctaa tgccagactg
     5641 gttgattttc aggcaacgct gccactggca cagatatggg gtggaggaga agtggcatct
     5701 gcagatggaa tgcgctttgt tacgccagtc agaacaatca atgccggacc gaaccgcaaa
     5761 tactttggta ataacagagg gatcacctgg tacaactttg tgtccgatca gtattccggc
     5821 tttcatggca tcgttatacc ggggacgctg agggactcta tctttgtgct ggaaggcctt
     5881 ctggaacagg agaccgggct gaatccaacc gaaattatga ccgatacggc aggtgccagc
     5941 gatcttgtct ttggcctttt ctggctgctg ggataccagt tttctccacg cctggctgat
     6001 gccggtgctt cggttttctg gcgaatggac catgatgccg actatggcgt gctgaatgat
     6061 attgccagag ggcaatcaga tccccgaaaa atagtccttc agtgggacga aatgatccgg
     6121 accgcaggct ccctgaagct gggcaaagta caggcctcag tgctggtccg ttcattgctg
     6181 aaaagtgaac gtccctccgg actgactcag gcaatcattg aagtggggcg catcaacaaa
     6241 acgctgtatc tgcttaatta tattgatgat gaagattacc gccggcgcat tctgacccag
     6301 cttaatcggg gagaaagccg tcatgcagtt gccagagcca tctgtcacgg tcaaaaaggt
     6361 gagataagaa aacgatatac cgacggtcag gaagatcagt tgggagctct ggggctggtc
     6421 actaacgccg tcgtgttatg gaacactatt tatatgcagg cagctctgga tcatctccgg
     6481 gcgcagggtg aaacactgaa tgatgaagat atcgcacgcc tctccccgct ttgccacgga
     6541 catatcaata tgctcggcca ttattccttc acgctggcag aactggtgac caaaggtcat
     6601 ctgagaccat taaaagaggc gtcagaggca gaaaacgttg cttaacgtga gttttcgttc
     6661 cactgagcgt cagacccctt ttgatcccaa tttttccacc cagaaagctg acacaacacg
     6721 aatgatggtg ccttttttcc ggcagttctg ggatatgggc gcaaaggata aagcgaccgg
     6781 aaaatcacgg agtgatgtgc agcaacgcat tcagcagttt cacagtcagg aatttttaaa
     6841 ctcactccaa ggcacaacac aatttgcggg taccgattac cgcagcaaag acctcacacc
     6901 gaaaaaatca aggctactgg ctgacaccat ttcagctgtt tatctcgatg gctatgaagg
     6961 cagacagtaa gtggatttac cataacgcgt cattacgcgc accgctcaga cgggctcagg
     7021 gcgtttccgg cggctacggg ataaaacagg caacaacccc cccggaaaac tgccggaaac
     7081 gcgcccgata aattttatcc taatgcatgc ctatgcacgc aactgcatgg catatgtttt
     7141 gacgtaaaat ggatgccgta agcccttttt taccgacttt ctgcttcatc agcctgactg
     7201 acacgaccat attctttcag accgccccct gctaaacttc cttcatattg ccgcattaac
     7261 ccgggaggct ccgcaatgag caacactaca cactacgaaa acgccaactt tcttcgggaa
     7321 ctggctgaaa gcctgccccg catactgccg gaaggtggcc cggacaaagc tgcgctgttg
     7381 cagcgactgg ctaacgaaga actggcccag gcagagtatg aagatcaggt tcgcgccaaa
     7441 gtgaccgccg cccgtgctga cacgcgtccg ggtatgacca cggaacaact ccgccagcgc
     7501 ctgcatggcc gttatcagga actgcgcgat gccgtatgac gtggaatgga agcagggagc
     7561 catcgaggat gtaacgacgc tgtttgacta tatcgcggaa aattcatccc tgtgggatgc
     7621 gcagaacgtt accgaccggg ttctcgctgc ggcggacaaa ctggccgact tcccgcgact
     7681 ctatgaagtg gactcacgct atggcgagga cgttcgccgg atcagtctga tgggacagca
     7741 tgtcctctat gatgttgatg accagaccag aaaagtccgg gttctggccg tggtcggcca
     7801 gcgccagaat ccccatattg ttctctaata accggtgacg gggacgcaat tcattgcgtg
     7861 gccgtcgccg gttggacaag cacagcgcgt cagcgttttc cccacggcca ccaccggcgc
     7921 ggtgttccgg ccggggtctc tgctgtctcc ggtttgtgtt ccagcagtct catggactgc
     7981 atttcctgcc gtagcccggc cacctcctcc ctgagtgctt ttatctccgc gatcaacgcg
     8041 tcggaggcca ttttttcagt ctcacccgac agtctcaact cgtcccgctc gtgagtctca
     8101 tcctgtctta actcaccgta cacccggata agctcgctgg tatccaccac ccggccgcca
     8161 tcagcttccg tacggtatga tacgcggcct ttcgccatat cccgatacag ggaacttcgg
     8221 gctttacccg tcagttccgt cgcctgtctc accgtcaccc acgccattgt ctcaccccgc
     8281 tatttttgag acagtatacc tgacaggcgc acaccatgcc cggaaggaaa gaatcatgac
     8341 aaaacaggaa aaaacagccc tcaatatggc taaatttatt aaggagcagt cgctgctatt
     8401 gctcgataaa ctgaatgagc tggatatgga taccccggcc gacatgtgcg agcggctaca
     8461 cgaggatgcg gaaacactct ggcaggtgat gcgggataag ctcggggagg aataacagca
     8521 ccggcatggc cggtgctggc atttattttt tgccgaacgg ccaccaccgg cgggcaggtt
     8581 tgtcctccgg cagttcaggt accggcacgt taaaatagcg gctgttatat tcccgcatca
     8641 gctccgcttt cagcttctcg ttcaccgcgc tttccccgtg ccgctcactc accacgtcaa
     8701 tcatcgcgtt aatctgctcc ggtgagcact tcccggcatc aaccacctgc acaccgttct
     8761 cgatggcgaa ccgctccacc ttgcgataga ggtaatcaca cagcttcatt cctgtgggtt
     8821 tgttccgttc ccattgcgga tttttaccag tgaaaaagta cgtggctttt tcgtcgctgt
     8881 tgagccgctc gtcatacatc ccgatggatt tcatcatcgc cagatgctgg gcccggtcga
     8941 caaaacagaa aacatacgcc ccggtctgcc aggcgagcac caccatatct tccagatgct
     9001 cgctggtcgg catgatgcag cgctcgccgt tccggtcctc cgcgtcttca ataaatacca
     9061 tgtacagcgc cataatccct cactccgttg tcataaattt aaagcacgca aacaaccgtt
     9121 cacgatccag ctctgccgtt ttgcgggcct cgccggtctg tgggttcacg atttcatcca
     9181 ccgacaggat tgcccgctgc accctgacct gcgtcgcccg gtccgggcag atgtaccaga
     9241 tttcatcgat gccgttagct ttgcggttga acagataccc ggacaccact tcctgatacc
     9301 ggcgacggct cttgacggtg cgctccacct caaacgccac cgttttaccg tccagctccg
     9361 ccagggcgtc cggcactttc atgcctttaa gactcagacg gtgcagaatg cgccagccgg
     9421 tggcccctct ggcttccata tcaaggcgca gggattgcac ggccagccgg tgcggcagat
     9481 gtgctgccga cacccttgac ggctgaaacg gaataacatc cgccagcggt tcgtcctcgt
     9541 cgaacgagaa cgccacgcca tgcggcgtca gcccccacac gctgacctga cggccgtcca
     9601 ccggatattt ggtccggacc agcagccctt tcgccaccag gctgtcgagg aagcggtaga
     9661 ccggctgcac cgccgcaatc cccagccgcc gcgcaaccag ctccgccgtg gaacacagct
     9721 cttcccgcaa ccagcgcagg gtcagccggg ttttctccgc gttccgcgcc tgtcgtttcg
     9781 cgtggctgac gttgtacagc tccagcgcca tcagtaaatc cccgcaaaat catcatccgc
     9841 tttcggcccg ccgccgtcgg ccgggggccg gaactccgcc aggggatcgg ttttcgccag
     9901 caccgggaaa cgctgcaacg ggatttcccg gcgctcaacc cgcagcggac tgctgaacgc
     9961 catccgggcc acacccagcc cggtcatgac cgcgaacccg tccggcaggt gttgcagggt
    10021 gttcacgtca aacagcggcc gcgcctcctt ctggatgaag ctgtccccgc tgatatgctc
    10081 cacgttcccc gcctcccggc tggcacggcg gcgctccacg tccaccctga tttccccggt
    10141 ctgccccgcc gcccactgcg cggactccag gctggccgcc cggtaaaacc agcggatcgg
    10201 cgtgttatcc agtaccgtgg ttttcacaaa ttcagcgggc agatcctgcc cgcactgccc
    10261 gaaatcgccc agcgactggt gcgcgagcaa cagattgcag tcccggctgc gcagcgtccc
    10321 cagtgcgttc agtacatact tcgacagcag atatttaatt tcatcgagca tcacgctgac
    10381 gtgtggccac tcccggaact cgtcccgcgc aatgatgatt tgcgcgcagc gggcaaacag
    10441 cattttttgc acacggatca ccgcctcgtc gtccatcgag ccgataacgt acaggcagcc
    10501 gccgccgttg ataatccccg ccacatcgat gccactgtcc gtctgcagga cgctcaggtc
    10561 agcgacgttt tccagcctag tgatcaaccc cttcacgtcc tttttcaggg cctccggcag
    10621 cgcgtacgcc gccgccagca cctgctgaat gtttgccccc tgcggaaact gctccgcaat
    10681 cagcttcgcc gctttctgct cgctgatgcg gtagtggtcc gccacgtcgc cggtctcgcc
    10741 caggttaagc ccggcaatca gcaggttttt cagcgcgtac tggtcgagat cgcggaacag
    10801 attcagctgc gccggtttcc ccggccgcag atccagcagg gtgaagtccg gacagtgcgc
    10861 ccggaagacg tgcggcgccc atgcgtcccc tttggggtcg aagtacacca ccgcgtcccc
    10921 gaaatcgcgt acgcagcgaa tgagcgcgtt ggtggccatc acgctcttgc cgctcccggg
    10981 caggccgagg atctgaatgt tggtcttgcg ccaggtatca cgcggaacca gcaccggctt
    11041 gccctgctca tccttaccca gaaacaccgc ctgctcctgc cgggcctgct ccgaggaaaa
    11101 atcggccagc gcattaaccg gtaccggctc cccgaaatgc aggtggcgca cgtcggtcat
    11161 ctctcctgtc cgctggctgc tgcgccggct ctcctgattc agtttcgcgt tcaccgcctc
    11221 gtcagcagcc gcaaaatgac cgtcaagcag caggaacgcc agcagccgcg ccaacacaat
    11281 cccgacagcc agcccgcccg cgcaccaggc aatcccgaac aggttagccg gctgcagcag
    11341 gtaataatct gcgtttgcgt agaggtccgg acgcaggacg gtttctcccc agctctcgat
    11401 aaacacggcg acaaccagcc cccccagaag ggccagaccg gccgcgccgg tcagccagta
    11461 cgcgtagttc cagacatgtc gacgtgccct gaccatccgc ccgggcgaat ccagcgaggc
    11521 cagcaccgaa aatggcgctg gccgtttatt atcgcgctta tagcgatcgg catactcccg
    11581 tcgctcctta tgactcagcc ccacaaaacg catccgtccg gcccaaagtt cagccagacc
    11641 gaaaaacagg ccgataacta ggaaagcccc ggccagaccg taatactcct ggtacagcca
    11701 gctgacccac ggataaatct gccgggccag ctccggaatg tgccacttcc aggcaagcga
    11761 atccgtatta aacatgttct ctctccttca gacgatggtt tgccggcgtg atgtgcagcc
    11821 cccgctgcac aagccgcgga catgccccaa ggcatggcgg cggcggtcac gtccgtggtg
    11881 attgcgttca gctttcacca cggaggccag cctctttccg ccccatgcgg aaaaggcgca
    11941 gccccggagg aacaacgagc acgccccagc gtgtgagagg aaaaggcggc accccagtgc
    12001 ggtcttttcc ggtgttccgg cccccgtcag agttcgccat agcgagcggc tgaccacccg
    12061 caaaatcagg agcagctttg ctgcttcgcc aggtgcgtcc agcaccgtgg atttttgtgg
    12121 gtggtcaggc gtgagcctga cgggggccgg gtgaatttct ggcgtggccg gcgtccagcc
    12181 ggacggagcc agaaagaggg ggagagaccg tgcgtcggtg gcgtccagcc agcgtaagca
    12241 ggggatatcc ccctgaggtc agccacgccc tcgcggggat gacctgtatt tctccgtaag
    12301 gagaaaaaag caacagagat aggttaacgt aaaatgttca gcgaaaaacc gatatgattc
    12361 acctatcctg gtaatttatt atcagggaat ataaagactt aatcagggga caatatgcca
    12421 catcagcgcg tccggtgtcc gtcttgtagg tgccggggaa attgcgcctc ctcgctcact
    12481 cggtcgctac gctccggccg tgagactgcg gcgggcgctg cggacacatg caaagttacc
    12541 cacagattcc gtgaataagc aggggactaa catgtgaggc aaaacggcaa ggccacgcag
    12601 gtggcgtttt tccataggct ccgccctcct gactgagttc acataaacag acgctttttc
    12661 ggtgcatctg tgggagccgg tggctcaacc atgaatcagt caggagtggc gaaacccgac
    12721 aggacttaaa gatcatcacc gtttccgatg aatcgctccc tcttgcgctc tcctgttcca
    12781 accctgccgt ttcccggata cctgttccgc ctttctccct tgcgggaagt gtggcgcttt
    12841 ctcatagctc acactctggt atctcggctc ggtgtaggtc gttcgctcca agctgggctg
    12901 taagcaagaa ccccccgttc agcccgactg ctgcgcctta tccggtaact gttcacttga
    12961 gtccaacccg gaaaagcacg caaaacgcca ctggcagcag ccattggtaa ctgggagttc
    13021 gcagaggatt tgtttagcta aacacgcggt tgctcttgaa gtgtgcgcca aagtccggct
    13081 acactggaag gacagatttg gttgctgtgc tctgcgaaag ccagttacca cggttaagca
    13141 gttccccaac tgacttaacc ttcgatcaaa ccacctcccc aggtggtttt ttcgtttaca
    13201 gggcaaaaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctact
    13261 gaaccgcgat ccccgtcagt tcagaggagg acgatggtgc caccgttcca ccctgaacat
    13321 cccggatata gtttgcctga catgcgacca gaagaaagcc tgaaaaaatc aatataaaaa
    13381 gaaaaacaac ctttaccttt ttcatatacc ttccctgtat ttaaaaaccc cggtattcat
    13441 ccctgaacac cggaagtctg tatttcaaat catccttgat aacttttgct taccagttac
    13501 catcccggta aacaaaggat acaaagtaat caaatttact aatatcgatt acatttttaa
    13561 agtgtggctg taatatcgaa acccatgatt tttcaacatc aaaccaacca ttattaatat
    13621 tatcctttaa aggcatcccc agactttcaa tgacagaacc atcatcaccg aagtcttttg
    13681 agtattcacc acctttaaac tcttcggttt tcttatcaaa ccaatgaata tgtaatttaa
    13741 gccccatatt tacctcttaa agatattttt taatgtttcg ttttggatcc ggccctttaa
    13801 cctgcttacc cgtttttgga tcgaatgccc cgaggtgttc g
//
