LOCUS       CP011627               14274 bp    DNA     circular BCT 09-JUN-2015
DEFINITION  Klebsiella oxytoca strain CAV1374 plasmid pCAV1374-14, complete
            sequence.
ACCESSION   CP011627
VERSION     CP011627.1
DBLINK      BioProject: PRJNA246471
            BioSample: SAMN03733738
KEYWORDS    .
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 14274)
  AUTHORS   Sheppard,A.E., Stoesser,N., Wilson,D., Sebra,R., Kasarskis,A.,
            Anson,L., Giess,A., Pankhurst,L., Vaughan,A., Grim,C.J., Cox,H.,
            Yeh,A., Sifri,C.D., Walker,S., Peto,T.E., Crook,D.W. and
            Mathers,A.J.
  TITLE     Rapid spread of a carbapenem resistance gene driven by multiple
            levels of genetic mobility
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14274)
  AUTHORS   Sheppard,A.E., Stoesser,N., Wilson,D., Sebra,R., Kasarskis,A.,
            Anson,L., Giess,A., Pankhurst,L., Vaughan,A., Grim,C.J., Cox,H.,
            Yeh,A., Sifri,C.D., Walker,S., Peto,T.E., Crook,D.W. and
            Mathers,A.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2015) University of Oxford, John Radcliffe
            Hospital, Oxford OX39DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: HGAP3 v. 2.2.0
            Genome Coverage              :: 57X
            Sequencing Technology        :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 06/03/2015 10:35:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision :: 2.10
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes                        :: 6,915
            CDS                          :: 6,622
            Pseudo Genes                 :: 175
            rRNAs                        :: 25 (5S, 16S, 23S)
            tRNAs                        :: 86
            ncRNA                        :: 7
            Frameshifted Genes           :: 42
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..14274
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="CAV1374"
                     /isolation_source="Perirectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:571"
                     /plasmid="pCAV1374-14"
                     /geo_loc_name="USA:Virginia"
                     /lat_lon="38.14 N 78.45 W"
                     /collection_date="2010-08"
                     /collected_by="Clinical Microbiology University of Virginia
                     Health System"
     gene            254..1408
                     /locus_tag="AB185_00040"
     CDS             254..1408
                     /locus_tag="AB185_00040"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_001533040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AKL32375.1"
                     /translation="MPYLIRVLGFNNYGVFILTQAIAQYVLLFSDFGFNLSSTKKVARN
                     QNNELRVSIIFWSTIFGKFVLFLISFSFVFSFLFFFYDQNEYSILFLMFIPQMLAGVAT
                     PNWLFQGKERMKVLSLLTVSSKLIYIILIFSFVKSKGDLFYVALAQGASSFFVVLASLY
                     YVYKSRWVTMVIFDLRGVIINIRGSFVYFISVASVSLYTTLPTLIIGFILGESSVAYYN
                     IANTIRNAMQSLLNPIYQAIFPRVNNLYKSNKIDTVKFIKRSLFIIPCISLSFSIFIFF
                     TGQYLILFFAKEVTHEVVVLLKVLCFIPFISSICNILGVQSILVFGFKKYFTCITVLAG
                     LFNLATIYPLTISFGLVGSAISVLITELFILVSFFSFIKIKKIY"
                     /name="hypothetical protein"
     gene            complement(1511..1884)
                     /locus_tag="AB185_00045"
                     /note="transposase; disrupted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
     gene            complement(1929..2204)
                     /locus_tag="AB185_00050"
     CDS             complement(1929..2204)
                     /locus_tag="AB185_00050"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_004152333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="AKL32376.1"
                     /translation="MASVSVCCPSCSATQGVVRNGKSTAGHQRYLCSHCRKTWQLTFTY
                     TAYQPGTHQKIIDMAMNGVGCRATARIMGIGLNTIFRHLKNSGRSQ"
                     /name="transposase"
     gene            complement(2238..3250)
                     /locus_tag="AB185_00055"
                     /note="transposase; disrupted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
     gene            4123..4314
                     /locus_tag="AB185_00060"
     CDS             4123..4314
                     /locus_tag="AB185_00060"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_016947206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase"
                     /protein_id="AKL32377.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="RNA polymerase"
     gene            4314..4718
                     /locus_tag="AB185_00065"
     CDS             4314..4718
                     /locus_tag="AB185_00065"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="AKL32378.1"
                     /translation="MAKVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLETSE
                     LLRVFGAFAPVSVEQEEEQVSGQRDTVTSDRNEQVIYILKQEIEHLNTLLASKDSHIDS
                     LKQAMLLLENREEKRQNTTRPRAWWRFWQS"
                     /name="transcriptional regulator"
     gene            complement(4725..5423)
                     /locus_tag="AB185_00070"
     CDS             complement(4725..5423)
                     /locus_tag="AB185_00070"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_016947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AKL32379.1"
                     /translation="MTRTFILLCTGVFAIAYALYELKHMGVYYPDASNPQLFTGWKAAT
                     ELGPAILATAIAATAFSLWLFILFFRSSESRRIQSETDRYKQALREAEEAHAARQNALI
                     RDHNMKTETLHSHYCDAANVLREEYAQKESLLLHAVQNARKEARKELEEQREALNQRES
                     ILQKKELALETTLEKARKYVAGERHNTVLARLEAKKAEERRRNACATAERRRRKLEKLQ
                     TTDGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(5420..6307)
                     /locus_tag="AB185_00075"
     CDS             complement(5420..6307)
                     /locus_tag="AB185_00075"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="AKL32380.1"
                     /translation="MAKFASAARQATGVIKILQGDILRSVGTVRNYEQGLTRVAEWMKD
                     NRINHGDLRKLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQARILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLVPRQERAPDT
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSHLAERLEHVRLATPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="integrase"
     gene            complement(6300..6521)
                     /locus_tag="AB185_00080"
     CDS             complement(6300..6521)
                     /locus_tag="AB185_00080"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_016947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AKL32381.1"
                     /translation="MQFDTAAELAALQAQTCRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            complement(6693..7553)
                     /locus_tag="AB185_00085"
     CDS             complement(6693..7553)
                     /locus_tag="AB185_00085"
                     /EC_number="3.5.2.6"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_010331504.1"
                     /note="hydrolyzes the beta-lactam bond in antibiotics such
                     as the penicillins and cephalosporins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="AKL32382.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="class A beta-lactamase"
     gene            complement(7736..8293)
                     /locus_tag="AB185_00090"
     CDS             complement(7736..8293)
                     /locus_tag="AB185_00090"
                     /inference="EXISTENCE: similar to AA
                     sequence:SwissProt:P0ADI2.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="AKL32383.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase"
     gene            8457..11462
                     /locus_tag="AB185_00095"
     CDS             8457..11462
                     /locus_tag="AB185_00095"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_001496645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="AKL32384.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="transposase"
     gene            12052..12279
                     /locus_tag="AB185_00100"
     CDS             12052..12279
                     /locus_tag="AB185_00100"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_016162092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AKL32385.1"
                     /translation="MKLITVDESVVNAIVEYHAQGSEEAHAEGNRMFLRYLHELTGTQR
                     VAVEAARKQSLAAINKGGRFPVIKIAFMDG"
                     /name="hypothetical protein"
     gene            12899..13272
                     /locus_tag="AB185_00105"
                     /note="transposase; disrupted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
     gene            13493..14125
                     /locus_tag="AB185_00110"
                     /note="hypothetical protein; disrupted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
     ARG             complement(6697..7553)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(3599..4075)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.456"
                     /coverage="92.0"
                     /evalue="1.62e-177"
ORIGIN
        1 ataccctttc atcattaaag caagggagag aattccgttt ttaggtaggg tgatttatgt
       61 tttatttttt aacaatagca tctccgcaag atatgtaaat aatttcaagg gtaggttgaa
      121 gagctatatt aactgcttca tctcaaaata agcgggtagg aaagtgacct ttttttttaa
      181 aacaaatctt tttaagaata tcattcacct tacctttata caaggcgtgt catttttaat
      241 cccattaatt acaatgcctt acttgattag ggttttgggt tttaataatt atggggtgtt
      301 tattttaacc caggccatcg ctcaatatgt gttgttgttt tctgactttg ggtttaattt
      361 atcctcaaca aaaaaggtag ccagaaacca aaacaatgaa ttaagggttt ccattatttt
      421 ctggagcaca atatttggta agtttgtatt attcttgata tccttctcct tcgttttttc
      481 ttttttattt ttcttttacg atcaaaatga atatagcatt cttttcttga tgtttattcc
      541 tcagatgtta gctggggtag caaccccaaa ctggttattt cagggaaaag aaaggatgaa
      601 ggttctttca ctgctaactg tttcatctaa attaatatat attatattaa ttttttcctt
      661 tgttaaatcg aaaggtgatt tattctatgt tgcattagct cagggggcta gctcattctt
      721 tgttgttctt gcttcattat actatgtata taaatccaga tgggttacca tggttatatt
      781 tgaccttaga ggggtcatca ttaacataag agggtcattt gtctatttta tttccgttgc
      841 ctctgtaagt ttgtatacca cactgccaac attaattatt gggtttattc ttggagaaag
      901 tagcgtggcg tattataata ttgccaacac aataagaaat gcaatgcaga gccttttaaa
      961 tccaatatac caagccatat tcccaagagt taacaatctt tataaaagta ataaaatcga
     1021 cactgttaaa tttataaaaa ggtctttgtt tattattccg tgcattagcc tttccttttc
     1081 gatttttata tttttcactg gccagtactt gattttgttt tttgcgaagg aggttaccca
     1141 tgaagttgtt gttttgttaa aggttttatg ctttatcccc tttatttcat ctatttgtaa
     1201 tattcttgga gtccaatcta tattggtatt tggctttaag aaatatttta catgcattac
     1261 tgttttagct ggattattta atcttgcaac aatatatcca ttaacgattt cctttggcct
     1321 tgttggtagt gccatttcag ttttaattac tgagcttttt atcttggtga gttttttttc
     1381 attcattaaa ataaaaaaaa tatactaaac atctgttttt gaaaattatg ccaacctatt
     1441 gattggcata accgatgaga gaaacaaggg gggggggtac ccctattaag ttgtcggtaa
     1501 tgactccagt ttactgatag tgttttatgt tcagataatg cccgatgacg ttgtcatgca
     1561 actccaccga ttttgagaac gacagcgatt tcctgcccag tcgggcaaga tgctgcctca
     1621 gattcaggtt atgccgttca attctctgcg tgtaacgctt gctgataacg tgcagatttt
     1681 ccttcaggcg tgactcatac agcggccatc cgtccgtcat ccataccacc acctcaaagg
     1741 ccgacaacag gctcagaaga cattccagcg tagccatggt gcgttcaaga catgcgccac
     1801 caccgttctg cgtatcctgt cgtacgcgta aaacagccag cgctgacgtg attttgcccc
     1861 gacgtagccc cattgttcgt ccatctccgc acagacaatg acattactgc ccggttgtat
     1921 ccgggaggtt actgactgcg gcctgagttt tttaaatggc ggaaaatggt gttgagacca
     1981 atgcccataa tgcgtgcggt ggcgcggcat ccgacgccgt tcatggccat atcaatgatt
     2041 ttctggtgtg tgccgggctg atacgcggtg taggtgaagg tgagctgcca tgttttacgg
     2101 cagtgagaac agagataacg ctgatgcccg gcagtacttt tgccgttacg caccacgcct
     2161 tgggtagctg aacaggaggg acaacagacg gagacggaag ccacaggagc acctcaaaaa
     2221 caccatcata cactacatca gtaagttggt agcatcaccg tcttttgcaa tagtgcccac
     2281 acaatccgcg cattcttatt agccaatgct accgaggcaa tgttgttatt ccggcgtgcc
     2341 agtagttgac tggcccatcc cgatacagca tcctgtttac gtttgaccga ctgcaacaca
     2401 gccctggcac tgtgaatgag taaggttcgt aaataaatat cgcctcgttt gcttatcccg
     2461 agcaagactt gtttgccacc acttgaatgc tgacgtggaa ccagtcctaa ctaggcagcc
     2521 aactgtcggc tattttcgaa attgctggcg ttgccgatag tcgcaatgag tgcggtggcg
     2581 gtgatcgggc caatgcctgg gatcttgctg atacgctgac atagcgcatt ttggcggtag
     2641 cactgctcaa tctgcttgtc gagcgtagcg atgacatcga acagatatgt catatggtgc
     2701 ggtaacagac tcagctgtac acggaacaga gatggcaacg agttatccgc atcttctagg
     2761 atctcaggta actgtagttg tagttgtagc tgctggatcc ccctgggtac gacgatgcca
     2821 aattcagcca atagcccccg aatttgattc gcctgagcgg tccgttgctt gatgaaactc
     2881 tgacgactcc ggtgaagtgc caatacggct tgctgctcag ggggcttgac cggcacgaac
     2941 cgcatgttgg gtcgtgtgac ggcttcgcaa atagcatctg cagcatcatg cttattggtt
     3001 ttgacatagg gcttgacgaa ctgaggggcc atcagcttga cgttatggcc catcgacatt
     3061 agtttaccgg cccaaaagtg ggcagaagca caggttccat gccgatcaaa catggaggga
     3121 tgttggcaaa aaaggaaagc atttttgctc gtttgagttg cttgttgaac aaccgcttac
     3181 cgtgttcgtc aattccgtgg acctggaaaa cctcttttgc caaatcgatg ccgacagtct
     3241 taacgttcat gatggccgct ccgctcaagt aatgacgatt tatcgtccca ctgtggcaca
     3301 agatgtaggg gcgaccattc cattaacaaa gcccaaagcc acaggaggct ttctttttta
     3361 ttgaataata tgcgcatggg cattttcttt cttacgatct gcttgagatc ctgttttggg
     3421 acgcgtaatc tcttgctctg taaacgaaaa aaccgccttg cagggcggtt tttcgaaggt
     3481 tctcagagct accaactctt tgaaccgagg taactggctt ggaggagcgc agtcaccaaa
     3541 actgttcgtt tagtttagcc ttaactggcg cataacttca agactatctc ctctaaatca
     3601 gttaccagtg gctgctgcca gtggcgcatt tgcatgtctt tccgggttgg actcaagatg
     3661 acagttaccg gataaggcgc agcagtcgga ctgaacgggg ggttcgtgca tacagtccag
     3721 cttggagcga actgccttcc cggaactgag tgtcaggcgt ggaatgagat aaacgcggcc
     3781 ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc
     3841 catcaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg
     3901 agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggcaacaccg
     3961 tggctttttc cacaggctct tctcctcctg gttatagcga tgtctcttct gtgtgtcccg
     4021 tccgctcgcc gcagccgaac ggcaggagcg tagcgactga gtgagcgagg aagcggaaaa
     4081 gagacaggtt ttacactgag cactgacgct ctgaggcccc ttatgacgaa acaacagaaa
     4141 actgcgctga acatggctaa attcatccag aatcagtccc tgctcctgct ggagaagctt
     4201 aacgagcttg atctcgatgt tgaggccgac ctttgtgaaa aactccacga tgatgcggag
     4261 catcttttcc gtacactgtc cctcagactg gatgcgctac aggagggtga ctgatggcga
     4321 aagtgtcgat cagtgaggca gcacgcctga cgggtaagag caggacaact ctgcacaggt
     4381 taattaaagc cggtgaactg tccacctgtc ggggtgaacg gaatgcgaaa atgctcgaga
     4441 cttcagagct tttgcgggtg tttggtgcct ttgcgccggt atccgttgaa caggaggaag
     4501 aacaggtcag tggacagcgt gacacagtca cttctgaccg taatgaacag gtcatttaca
     4561 tcctgaaaca ggaaattgaa cacctgaaca cccttctggc ctcaaaagat tcacatatcg
     4621 acagtctgaa acaggcgatg ttgttgctgg agaaccggga agagaagcgg cagaacacga
     4681 cccggccccg tgcctggtgg cgtttctggc agtcctgaaa tgcctcagtg tttttgccct
     4741 gccgttccgt cagtggtctg gagtttttcc agtttccgtc tgcgccgttc cgccgtcgcg
     4801 caggcatttc tccgtctttc ctctgctttc tttgcttcca gacgggccag cacggtgtta
     4861 tgccgttccc cggccacata tttccgggcc ttctctaggg ttgtttccag cgccagctcc
     4921 tttttctgca ggatggattc gcgctgattc agtgcctccc tctgttcttc cagctccttt
     4981 ctggcttctt tccgggcatt ctgcaccgca tgaagcaaga ggctttcttt ttgcgcatac
     5041 tcttcccgca gtacatttgc cgcatcacaa tagtgactgt gcagggtttc tgtcttcatg
     5101 ttgtgatccc ttataagggc attttgtctg gcggcatgcg cctcttctgc ctccctcaga
     5161 gcctgcttat acctgtctgt ctcgctctgt atccggcggg actctgagct tctgaaaaac
     5221 aaaatgaaca gccacagcga gaacgctgtg gctgcgatcg cggtagccag gattgcggga
     5281 ccgagttcgg tggccgcctt ccagccagtg aacagctgcg gattgctggc atccggatag
     5341 taaacgccca tatgtttcag ctcatacagg gcgtacgcga tggcgaaaac accggtacac
     5401 agtaaaataa acgttcttgt catcgcaggt atgtctccgt gatttccgga cgaaaatggc
     5461 ccatttcctg gctgacggtg cgcagggcca tatcacgaac cagaccgagt ttttgcagct
     5521 cctgcatccg ttgctgggcg tagctgtgac gcacgccgtg tgccccccgt gaccagccca
     5581 gggtacgcat ggaggcggca ctgaaggcgt tcgaccagcg ctgtcctccg ccaatggcgt
     5641 actgactctg gtaaaacacg ccgcgatcgg tcacccggac cggggtcgcc agcctgacat
     5701 gctccagacg ctcagccagg tgtgacggca gcagaacttc cctgaccagg ccgcctttgc
     5761 cctgaacggt gtaaatcaca ccctcccgac cggcaaattt gctgtccgct gccggacggg
     5821 tatccggtgc acgctcctgg cggggcacga gggtcagcag ttcatgggcg cgcaatccgg
     5881 cggccagggc gatcgcggtg gccagggcgt tggcgggctg ttgtgctgcg gcaatcaggg
     5941 ccacctggtc cgggctgtag gcccggctgc tcagtatccg ggcctgctcc gatttcacca
     6001 ccggcaggtg ttcgccggca gggagggtct gcgtcacgtg gatcatcatg gcctggatgg
     6061 cctggcgttc catatcgagc gttttctggc cgacctgctc cccgcgctgc tccaggtagg
     6121 tgatggcctg cgccggtgtc agtttgcgta aatcgccgtg attaatccgg ttgtctttca
     6181 tccattctgc gacgcgggtc aggccctgct cgtaattccg gaccgtaccg acagagcgaa
     6241 gaatatcgcc ctggaggatt tttatcacgc ccgtcgcctg gcgggcagcg ctggcgaatt
     6301 tagccatttt tttccagcca gcgggtaacc gtggacaaca cgactttgat tctccgggcc
     6361 cggagccagc gttgcagctc cgctgcactg gccccggcct gatacaggct cagcagttcg
     6421 ccggtatagc ggtcaagacg ggagggacgg tagcggacct ggcggatacg gcaggtctgc
     6481 gcctgcaggg cggccagctc ggcagcagta tcaaattgca tctgtgctcc ggattgggtg
     6541 taagaggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6601 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6661 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6721 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6781 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6841 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6901 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     6961 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     7021 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7081 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7141 gtcagaagta agttggcagc agtgttatca ctcatggtta tggcagcact gcataattct
     7201 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7261 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     7321 accgcaccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7381 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7441 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7501 caaaatgccg caaaaaaggg aataagggcg acacgaaaat gttgaatact catactcttc
     7561 ctttttcaat attattgaag catttaccag ggttattgtc tcatgagcgg atacatattt
     7621 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7681 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     7741 aggccctttc gtcttcaaga attttataaa ccgtggagcg ggcaatactg agctgatgag
     7801 caatttccgt tgcaccagtg cccttctgat gaagcgtcag cacgacgttc ctgtccacgg
     7861 tacgcctgcg gccaaatttg attcctttca gctttgcttc ctgtcggccc tcattcgtgc
     7921 gttctaggat cctccggcgt tcagcctgtg ccacagccga caggatggtg accaccattt
     7981 gccccatatc accgtcggta ctgatcccgt catcaatgaa ccggactgcc acgccctgag
     8041 cgtcaaattc ctttatcagt tggatcatat cggcagtgtc gcggccaaga cggtcgagct
     8101 tcttaaccag aatgacatca ccttcctcca ccttcatcct cagcaaatcc agcccttccc
     8161 ggtctgttga actgccggat gccttatcgg taaatatacg gtttgctttc acacctgcgt
     8221 ctttgagtgc tctgacctga agatcaagag actgctgact ggttgagacc cgagcgtaac
     8281 caaaaagtcg cataaaaatg taccttaaat cgaatatcgg acaactcatg tctattatta
     8341 caaatttacg atttaataga catattaatg taacagtttt acgatgtccg ataatttata
     8401 acatttcgta cggttggaaa aatgttacta aatgcccgtc aggcagggag gccgatatgc
     8461 ccgttgactt tctgaccact gagcagactg aaagctatgg cagattcacc ggtgaaccgg
     8521 atgagcttca gctggcacga tattttcacc ttgatgaagc agacaaggaa tttatcggaa
     8581 aaagcagagg tgatcacaac cgtctgggca ttgccctgca aattggatgt gtccgttttc
     8641 tgggcacctt cctcaccgat atgaatcata ttccttccgg cgtccggcat tttaccgcca
     8701 gacagctcgg gattcgtgat atcaccgttc ttgcagaata cggtcagagg gaaaataccc
     8761 gccgtgagca tgcagcgctg atacgtcagc actatcagta tcgtgaattt gcctggccct
     8821 ggacatttcg ccttacccgt cttttatata cccggagctg gataagcaac gaacgtcctg
     8881 gcctgctttt cgatctggcg acagggtggc ttatgcaaca tcgtattatt ctccccggag
     8941 ccactacgct gacccggttg atttcagagg taagggaaaa ggcgacgttg cgcctgtgga
     9001 acaaactggc actgataccg tcagccgaac agcgttcaca gctggagatg ctgctggggc
     9061 caactgattg cagccgcctg tctttactgg aatcactgaa aaagggccct gtgaccatca
     9121 gtggtccggc gtttaatgaa gcaattgaac gctggaaaac tctgaacgat tttggcctgc
     9181 atgctgaaaa cctgagtaca ctcccggctg tgcgcctgaa aaatctcgca cgttatgctg
     9241 gtatgacttc ggtgttcaat attgccagga tgtcaccgca gaaaaggatg gcggttctgg
     9301 ttgcctttgt ccttgcatgg gaaacgctgg cgctggatga tgcattggac gttctggacg
     9361 ccatgctggc cgttatcatc cgtgacgcca gaaagattgg gcagaaaaaa cggctccgct
     9421 cgctgaagga tctggataaa tctgcattgg cgctcgccag cgcatgttcg tacctgctga
     9481 aagaagaaac accggacgaa tcgattcgtg ctgaggtgtt cagctacatc ccaaggcaaa
     9541 agctggctga aatcatcacg cttgtccgtg aaattgcccg gccctcagac gataattttc
     9601 atgaagaaat ggtggagcag tacgggcgcg ttcgtcgttt cctgccccat ctgctgaata
     9661 ccgttaaatt ttcatccgca cctgccgggg ttaccactct gaatgcctgt gactacctca
     9721 gccgggagtt cagctcacgg cggcagtttt ttgacgacgc accaacggaa attatcagtc
     9781 ggtcatggaa acggctggtg attaacaagg aaaaacatat cacccgcagg ggatacacgc
     9841 tctgctttct cagtaaactg caggatagtc tgaggcggag ggatgtctac gttaccggca
     9901 gtaaccggtg gggagatcct cgtgcaagat tactacaggg tgctgactgg caggcaaacc
     9961 ggattaaggt ttatcgttct ttggggcacc cgacagaccc gcaggaagca ataaaatctc
    10021 tgggtcatca gcttgatagt cgttacagac aggttgctgc acgtctttgc gaaaatgagg
    10081 ctgtcgaact cgatgtttct ggcccgaagc cccggttgac aatttctccc ctcgccagtc
    10141 ttgatgagcc ggacagtctg aaacgactga gcaaaatgat cagtgatcta ctccctccgg
    10201 tggatttaac ggagttgctg ctcgaaatta acgcccatac cggatttgct gatgagtttt
    10261 tccatgctag tgaagccagt gccagagttg atgatctgcc cgtcagcatc agcgccgtgc
    10321 tgatggctga agcctgcaat atcggtctgg aaccactgat cagatcaaat gttcctgcac
    10381 tgacccgaca ccggctgaac tggacaaaag cgaactatct gcgggctgaa actatcacca
    10441 gcgctaatgc cagactggtt gattttcagg caacgctgcc actggcacag atatggggtg
    10501 gaggagaagt ggcatctgca gatggaatgc gctttgttac gccagtcaga acaatcaatg
    10561 ccggaccgaa ccgcaaatac tttggtaata acagagggat cacctggtac aactttgtgt
    10621 ccgatcagta ttccggcttt catggcatcg ttataccggg gacgctgagg gactctatct
    10681 ttgtgctgga aggtcttctg gaacaggaga ccgggctgaa tccaaccgaa attatgaccg
    10741 atacagcagg tgccagcgaa cttgtctttg gccttttctg gctgctggga taccagtttt
    10801 ctccacgcct ggctgatgcc ggtgcttcgg ttttctggcg aatggaccat gatgccgact
    10861 atggcgtgct gaatgatatt gccagagggc aatcagatcc ccgaaaaata gtccttcagt
    10921 gggacgaaat gatccggacc gctggctccc tgaagctggg caaagtacag gtttcagtgc
    10981 tggtccgttc attgctgaaa agtgaacgtc cttccggact gactcaggca atcattgaag
    11041 tggggcgcat caacaaaacg ctgtatctgc ttaattatat tgatgatgaa gattaccgcc
    11101 ggcgcattct gacccagctt aatcggggag aaagtcgcca tgccgttgcc agagccatct
    11161 gtcacggtca aaaaggtgag ataagaaaac gatataccga cggtcaggaa gatcaactgg
    11221 gcacactggg gctggtcact aacgccgtcg tgttatggaa cactatttat atgcaggcag
    11281 ccctggatca tctccgggcg cagggtgaaa cactgaatga tgaagatatc gcacgcctct
    11341 ccccgctttg ccacggacat atcaatatgc tcggccatta ttccttcacg ctggcagaac
    11401 tggtgaccaa aggacatctg agaccattaa aagaggcgtc agaggcagaa aacgttgctt
    11461 aacgtgagtt ttcgttccac tgagcgtcag acccctaaga acatcagtgg ccgtaaacgg
    11521 ttcactgcgg catgcgccga tcgccctcgt tcgaacctct cgtggttcag cccggactgc
    11581 tcgcggctga taacgtccct taagccaggg aatggcctga cctgattgcc ggtctcgcag
    11641 attttggctg ttttctcagg attctcggat tgggctgtcc ggtacagtat ccccgggggg
    11701 acccggctgt ttaacctgct gccggtaggc aggtgcgcgt cagagtgcgc agcctcatgg
    11761 tttgactaaa tactagccag gttttgcatg cgtgtgaatg ttgtttttta aatgttgcgt
    11821 ctgactccgc tcaatagcga cacccgctat gtacaggcgc atttatgcgt cgtacatagc
    11881 gggtgtcgca ggtgctcata ttgcctgaat aaagaacgcc gggactgacc gccccggacc
    11941 ccgccaacgg tgaccggaca gacaaaaaat ttgcctgccc ggtcaccgtt ttcacgccag
    12001 gatgaccgtc gtgatgccag gatggtgtca cagagaaagc ggagagacag aatgaagctg
    12061 attacggtgg atgaatccgt ggtgaacgcg atagtggaat accacgcaca gggcagtgag
    12121 gaggcgcatg cagagggaaa caggatgttc ctgcgttatc tgcacgagtt aacgggaaca
    12181 cagcgtgtgg cggttgaggc ggccagaaag cagtctctgg ccgcgataaa taaggggggg
    12241 cgcttccctg tgattaaaat cgcatttatg gacggctgat gtctgtgcac tggcccggaa
    12301 atatttttct ccggtgaggg gctgatatga gctacctttt tcttatcgcc ggcaccgcac
    12361 caggtgcgac ggtcctttta cagagttgtc ctcatttgac gggcctggat ggcccgttct
    12421 tttatgcttt taacgtccag aacccttttc ccgaccgaaa tcaccggcta tgcggtggtg
    12481 tgcatagcca tgcatatgcg taaaataaat caggtgcgtt tctggcgggt tttcgggtgg
    12541 tttgttgcct gttttaccgg tttcctgcca gaaacgccct gaggccgttt tcgcggtgcg
    12601 cgtaaaaagg ctttatgtta aataaaccat ctttacgtct tgttctgttt ttgatgcaag
    12661 ccgctcgttc agaagccggg tggttgatag tattaacgcg aaaccagaga gggatgctta
    12721 tgaacggtat tgaattggac tgaccccgcc ccggtagacg atcctgccct atagtttgag
    12781 cataggagga gcgtatgggc attggtctca acaccatttt ccgccattta aaaaactcag
    12841 gccgcagtca gtaacctccc ggatacaacc gggcagtaat gtcattgtct gtgcggagat
    12901 ggacgaacaa tggggctacg tcggggcaaa atcacgtcag cgctggctgt tttacgcgta
    12961 cgacaggata cgcagaacgg tggtggcgca tgtcttgaac gcaccatggc tacgctggaa
    13021 tgtcttctga gcctgttgtc ggcctttgag gtggtggtat ggatgacgga cggatggccg
    13081 ctgtatgagt cacgcctgaa ggaaaatctg cacgttatca gcaagcgtta cacgcagaga
    13141 attgaacggc ataacctgaa tctgaggcag catcttgccc gactgggcag gaaatcgctg
    13201 tcgttctcaa aatcggtgga gttgcatgac aacgtcatcg ggcattatct gaacataaaa
    13261 cactatcagt aaactggagt cattaccaaa gcacgctaca acactaaatt tgagtctcta
    13321 cagattttta tatgtgggct ttattatttt attgcgaatg ggtataatgt gattttactt
    13381 gtatggtttt agtagtacac acctgccttt caggcaaata ggaagtgttt tagttgcatt
    13441 taaaaaattg acaaacaagc atgttttttt caaattgcac atggagagta aaatgaaaat
    13501 ttcagtggca atggcaacgt ataacggtca aggatacata gcggagcaat tggattcaat
    13561 attgtgccag actcacgaag acattgaggt tgttatagtt gatgatatgt catctgacag
    13621 tactcttgat ataataaagt cgtattctga tcgcaggata aaacttataa gcaatactgt
    13681 taatctaggg gttagtggtg catttgaact ggcaataagt atgtgttccg gtgaatacat
    13741 agcactttgt gatcaagatg atatatggcg aaaaaacaaa ctggagtcac ttctctctag
    13801 cattggtagc gcatcattaa tatatagcga ttttgatttt atagacgcta atggaatgat
    13861 tatagaaaaa ccatcaaatt tcgttaatat actacactcc actgactcat caaataaatc
    13921 aattcataaa ctggcgttcc ttaattcatt cgtattaggg tgctctatga tgtttaactc
    13981 caatataaag aaagaagtgt tacctatact ggatgaagga tttaatcatg ataaatggat
    14041 tatcaatgta gcagtcaact taaatgggat taagttcaaa aatgaagttc tttttagcta
    14101 tagactgcat ggtaataatg catcaattaa aaaagaagaa aacaaagaca ttggattttt
    14161 aaataagata tataataaga cctttaaaga tagcaggagg gattttttta gttacaagtc
    14221 aactattgag ttgtgtaata ggtgtggaaa gtcaagttcg cgcatgatta agtt
//
