LOCUS       CP011626                6538 bp    DNA     circular BCT 09-JUN-2015
DEFINITION  Klebsiella oxytoca strain CAV1374 plasmid pCAV1374-6538, complete
            sequence.
ACCESSION   CP011626
VERSION     CP011626.1
DBLINK      BioProject: PRJNA246471
            BioSample: SAMN03733738
KEYWORDS    .
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Sheppard,A.E., Stoesser,N., Wilson,D., Sebra,R., Kasarskis,A.,
            Anson,L., Giess,A., Pankhurst,L., Vaughan,A., Grim,C.J., Cox,H.,
            Yeh,A., Sifri,C.D., Walker,S., Peto,T.E., Crook,D.W. and
            Mathers,A.J.
  TITLE     Rapid spread of a carbapenem resistance gene driven by multiple
            levels of genetic mobility
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6538)
  AUTHORS   Sheppard,A.E., Stoesser,N., Wilson,D., Sebra,R., Kasarskis,A.,
            Anson,L., Giess,A., Pankhurst,L., Vaughan,A., Grim,C.J., Cox,H.,
            Yeh,A., Sifri,C.D., Walker,S., Peto,T.E., Crook,D.W. and
            Mathers,A.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2015) University of Oxford, John Radcliffe
            Hospital, Oxford OX39DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: HGAP3 v. 2.2.0
            Genome Coverage              :: 57X
            Sequencing Technology        :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 06/03/2015 10:35:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            Annotation Method :: Best-placed reference protein set; GeneMarkS+
            Annotation Software revision :: 2.10
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes                        :: 6,915
            CDS                          :: 6,622
            Pseudo Genes                 :: 175
            rRNAs                        :: 25 (5S, 16S, 23S)
            tRNAs                        :: 86
            ncRNA                        :: 7
            Frameshifted Genes           :: 42
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="CAV1374"
                     /isolation_source="Perirectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:571"
                     /plasmid="pCAV1374-6538"
                     /geo_loc_name="USA:Virginia"
                     /lat_lon="38.14 N 78.45 W"
                     /collection_date="2010-08"
                     /collected_by="Clinical Microbiology University of Virginia
                     Health System"
     gene            complement(1500..2105)
                     /locus_tag="AB185_00020"
     CDS             complement(1500..2105)
                     /locus_tag="AB185_00020"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA resolvase"
                     /protein_id="AKL32371.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="DNA resolvase"
     gene            2200..5097
                     /locus_tag="AB185_00025"
     CDS             2200..5097
                     /locus_tag="AB185_00025"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="AKL32372.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEVMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="transposase"
     gene            complement(5211..5633)
                     /locus_tag="AB185_00030"
     CDS             complement(5211..5633)
                     /locus_tag="AB185_00030"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_000135007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AKL32373.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="hypothetical protein"
     gene            complement(5633..5824)
                     /locus_tag="AB185_00035"
     CDS             complement(5633..5824)
                     /locus_tag="AB185_00035"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase"
                     /protein_id="AKL32374.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="RNA polymerase"
     Typing          5873..6337
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.26"
                     /coverage="91.0"
                     /evalue="7.770000000000004e-156"
ORIGIN
        1 tggatctcaa gaagatcctt ttaaagtcca aaaatttccg ttctccacac tacccaccat
       61 gagcacttat caatcataaa aatcatgata ggccactgaa tcggcaaggg tttaacagac
      121 acatcagagt catttaagat gacttaacgc tccaatagac ggtgtaagtt aactgtcgct
      181 gccgcgcttt acggcagtgg ttgcagacaa gcagaggggg catacccaca actcatgcac
      241 ctttttttgt ttttcctgta tgtggggttt gacgctcaac gtaaaggttt cacacgttaa
      301 ggggtttttg tccgataaat cagaatattg tggacagtga aaaatgtgcc taaagcggtg
      361 tgtttttaat cgtcaaatag cacttaacgg acatcatttg tccattaacc gatttttcag
      421 acgacttttc tcgcctaacg tgagttttcg ttcgcctgag catcagccac ctatgtacaa
      481 aaaatataac taggaataat ctcaataatc ttcatcaaga tacgcaaaaa aaacgataat
      541 gcttcatctg acgacaacaa acagcgacaa tgtcatgtta cgaaaaagtg aaattatctt
      601 aataaaatca tgtggtaaat caaaaacaaa aattttttta cgaggtgtgg aggagggaat
      661 aatggttgat gcaagttttg aagatataaa atttgctatc aacaataacc tccacgatct
      721 tctcgggatc caagccgaaa ccacagaact aaaactagca aagtgataca gcacctgaag
      781 catgaaggtt cgaagtaaag caacatatta aacataacat ccgttatcag caccgcctga
      841 gaggctctca gggcgtttct gacgggaaac cggcaaaaca ggcaacaaac cacccgaaaa
      901 cccgccagaa acgcgcaggc tctgttttta cggcatgcat gagtatgcgc acaagtgcat
      961 agcgcggaaa ttgacaccat tcagccaggg taagtcctgt ttccggcgtt cttcccatcc
     1021 gaaaacggtg aacggggacg caatttattg cgtggccgtg aagcgttgcc ggcgcagccg
     1081 gaaaaccggt tttcagcacg ttcaggaatc gggttcgtcg acggccggca atcacgttat
     1141 ggcaagtcgc cggacgactc accacacgat tcaccggcga ttcgaatcgt gaattgaatc
     1201 aagagtcacc atgaacaaaa aacgcccaaa ttgatgaatt gaatcaagag tcgattcatc
     1261 ataacaacat gataataaag cgtgattcac atatgaatcg tgggcaattc gtaaacgatt
     1321 cgccatagag tcgtccacga cttggatctg cactttgtaa cacatcactg cacattaatt
     1381 tttcattaac caattgtttt aaataaggaa tctgcaaaat tttaattttg cgtagtgtgt
     1441 gggtattttt agtcgtgagg agcgttgggg tcggttccgg ctgagggcga aatgacaccc
     1501 taagcgttag ctctgtgtcg ttgcacgatg tcagcgacgg tattcttgct gataccgagc
     1561 tcgcgtgcga tccagcgata gctgcgtccc tcggccctca tcgcaaccac cttaggcaaa
     1621 agtcggtctg attttggtcg cactccggcc tgacgaccaa gcctcttacc acgtgccttc
     1681 gcaacagcaa ggcctgactt gacccgctcg ctgatgagat cccgctcaaa ctccgcaatg
     1741 ccggaaagaa acgtcgccag cattcgtcca tacggcgacg aaagatcgaa cgccattcca
     1801 ttcatggcta tcacggaaac cttccagttc tccagttcac gtagcgtatt gagcagatcg
     1861 agcgtcgagc gcccccaccg ggaaagctca gtgaccagga ttgcatcaat ttgtctggac
     1921 tgggcaagcg ccaggacttt ctttcgctcg gcccggtcga gtttagttcc tgaacctgtt
     1981 tccttaaata ttcccaccac gtcgtagccg gcacggccgg cgaaggctcg cagatcaaat
     2041 tcctggcgtt cacaagactg atccgctgtt gaaacccggc agtaaatggc ggcacgatgt
     2101 cccaattgaa ccctcctgga tttttgtatc ggaacgccct gatttatatg ggctggctgt
     2161 tgtccaaaac agactatact tcaaaaggga cgaatttgta tgtcacgacg ccatattttc
     2221 accgaacggc agcgagcagc gctgttcgat ctgcccacgg acgaactgtc gctactgaag
     2281 ttctacacgc tgggcgatga tgacctggaa aacattaggc agcgccgcag accggaaaac
     2341 aggattggct ttgccctgca actttgtgcc ttacgatatc cgggccgtgc actggctcct
     2401 ggtgagatga tcccgcgtga agtcctttcc ttcgtcggtg ctcagcttgg agttccggct
     2461 gatgcgcttc tcacttatgc cacacggcgc caaacccgtc agcagcacat ggacacgctg
     2521 cgcgaaattt acggctacaa gaccttcacg ggccgtggtg cccgtgatct gcgggagtgg
     2581 actttcggcc aggccgaaga tgccagatca aacgaggatc ttgctcatcg ttttattgtg
     2641 cggtgtcggg aaacttccac cattctgccc gcagtatcga caatcgagcg cttgtgcgcg
     2701 gatgctctgg tcgccgctga gcggcggatt gaaacgcgga ttgcggaaaa tttaacagcg
     2761 gatgttcgcg atcacctgga caaacttctg agtgaaatgc tcgccggcaa tatcagtcgt
     2821 ttcatctggc ttcgcaactt cgaggttggt aacaactcgg ctgctgctaa ccgtttgctc
     2881 gacaggctcg aatttctgcg taccctgaat atcaatcata gtgctttggc cagcatacct
     2941 gcccatcgca ttgcccggct gcgtcggcag ggtgaacgct acttcaccga cggtttgcgt
     3001 gacatcactt cggaccgccg ctgggcgatc cttgccgtct gtgttgtgga gtgggaagcg
     3061 gcgattgctg atgccatagt cgaaacccat gacaggatcg taggaaaaac ctggcgggaa
     3121 gcgaagcgcc agcatgacga aacaatttcc ggctctaaag ccacactcac ggatacgatc
     3181 cgtaccttca ccgcgctggg agcttcgttg cttgaggccc gcagtgacgg aaccccgctg
     3241 gagatggctg tcgccagttc ggttgcatgg gaccggctcg ctcaactggt agcgacaggg
     3301 actcaactca gcaacacgct agccgatgag cctcttgcat atgtcgggca gggataccat
     3361 cgctttcgtc gttatgcgcc ccgcatgttg cgctgtctga agctcgaagc cgcgccggtc
     3421 gccggaccat tggtagcagc agctttgtcg atcggagaga tgaaaggtgt tgcatcgcca
     3481 gaaaggcgtt tcctgcggcc cagctccaaa tggaaccgtc atttacgagc tcaggaaaaa
     3541 ggagataccc gtctttggga agtggcggta ctctttcacc tccgggatgc ttttcgttcc
     3601 ggagatgtct ggctcgctca ttcgcgccgc tatggtgacc tcaagcaggt actggtgccg
     3661 atgatcgcgg cgcaggaaaa tgcaaaactg gccgtgcctt ccaacccaca ggattggctg
     3721 gcagacagaa aggcgcgact cacgatcgct cttaagcggc tggcccgggc tgcccgtaac
     3781 ggcactattc cgcacggtag catagaagat ggaacgttgc ggatcgacag gttgacagca
     3841 gacgtgccgg atggtgccga ggcactcata ctggatctgt atcgccgaat gccgtccgtt
     3901 cggattaccg acatgctgct tgaagttgat gcagcccttg gtttcacaga tgcgtttacc
     3961 catctgagaa ccggggctcc atgtcgcgac cggatcggtc tgctcaacgt cctgctcgct
     4021 gaagggctca atctgggcct gcgtaagatg gcggaagcta caaacacgca tgattactgg
     4081 cagctctcac gccttgcccg ctggcatgtt gaaagcgaag ccatgaacca ggcattggca
     4141 attgtggtgg ccgcgcaggg taaactgccg atgtcacgcg tctgggggat gggcacgtca
     4201 gcatcgagcg atggtcagtt tttcccgaca gcgcggcatg gcgaagtcat gaacatggtc
     4261 aatgccaaat atggttctgt tcccggcctc aaagcgtata ctcacgtaag cgaccagttc
     4321 gcgccattcg cttgtcagtc gatcccggcg accgtgagcg aggcaccgta tattctcgat
     4381 ggactactga tgaacgaggt cggtcgccat gttcgcgaac agtatgccga tacagcagga
     4441 ttcaccgacc atttgttcgg agccagtagc ctgctcggct acaatctcgt tctgcgaatc
     4501 agggatctgc catcgaagcg gttgtacgta tttaatcccg atacgacccc cagggagtta
     4561 cgcaagttgg taggtggaaa agcccgggag gatcttatcg ttgcgaactg gcctgatatt
     4621 ttccgttgtg ccgcgacgat gaccgctggc aaaatcaggc ccagccaact cctgcgcaag
     4681 ctcgcttctt acccacgaca aaacaacctt gcagttgcgc ttcgtgaagt tggtcgtatt
     4741 gaacggaccc ttttcattat tgagtggatc ctggatacgg acatgcagcg gcgtgctcag
     4801 atcggtctta acaagggaga ggcccaccat gcgctcaaaa atgcgctccg tatcgggagg
     4861 cagggggaaa ttcgcgatcg cacgacagag gggcagcact accgaatcgc tgggctcaat
     4921 ttattgactg cggtgatcat ttactggaat accgtccatc ttggtcatgc cgtcacggag
     4981 cggcggaacg aagggttgga tgttccccct gaatttcttc cccacatatc cccattgggc
     5041 tgggcgcaca ttctactgac tggcgaatat ctttggccca aggaaccgaa agcttagggt
     5101 gtcatttcgc cctcagccgg aaccgacccc cgttcagaga tctggcgatt tttgggaagg
     5161 ttcaggaact ccctgggatg gccggagagg aaaactcaag catggcagga tcaggatttt
     5221 ttccagaatt tccaccacgg ggaatggggt ggtgaagaag gctcgcgctt gtgctccaga
     5281 agctgcatag cctgacgaag ggaatcaata tgctcatctc gagccttaac agccccttta
     5341 agaccatcgt tttcagcttc aagtagagcg attctatgtt gtaaatcatg taaaccaacg
     5401 ctgttaatac ttgtagattc tgcgtcactt tttacatcat attttacagt atcgatgaca
     5461 ccatcaagag aaccaaacac tctaataagc tctgaagtat caataacttt aatagccttc
     5521 ccgtgcttta caacagaggt gtaacttaac ttcccactgt taatcatctt gtaaattgtg
     5581 ggacggctaa ccttaacaag ccgtgccgct tcactaatag acactctggc catcaattac
     5641 catcctgtaa ttcatccagt ctcgaggaca gtgtacggaa aagatgctcc gcatcatcgt
     5701 ggagtttctc acaaaggtcg gcctcaacat cgagatcaag ctcgttaagc ttctccagca
     5761 ggagcagaga ctgattctgg atgaatttag ccatgttcag cgctgttttc tgttgtttcg
     5821 tcataaaggg cctcagagcg tcagtgctca atgtcagaca gatctctttt ccgcttcctc
     5881 gctcactcag tcgctacgct cctgccgttc ggctgcggcg agcggtcgca acgcgcacat
     5941 aacgacatag agcagacagc tcggagaggg agaaggcagc acagcggccg tttttccata
     6001 ggctccgccc ccctgacaag catcacagaa tttgacgctc aaatcagtgg tggcgaaacc
     6061 cgacaggact ataaagatac caggcgtttc cccctgatgg ctccctcgtg cgctctcctg
     6121 ttcctgccat tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     6181 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tgggctgtgt gcacgaaccc
     6241 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6301 agacacgact aatcgccatt gacaacggcc actggtagac aggatgtgcg agagatgtat
     6361 cgagagttct tgaagtggtg gcctgactgc ggctacactg aaaggacaga tttggtgaca
     6421 tgtctcgcac aagactgtta ccacggttca gaagttcccc aacttactga accttcgaaa
     6481 aaccacctcc ccaggtggtt ttttcgtttc ttggataaga gactacgacg acaaatga
//
