LOCUS       CP006928               14249 bp    DNA     circular BCT 26-JUL-2016
DEFINITION  Klebsiella pneumoniae 30660/NJST258_1 plasmid pNJST258N4, complete
            sequence.
ACCESSION   CP006928
VERSION     CP006928.1
DBLINK      BioProject: PRJNA226698
            BioSample: SAMN03081502
KEYWORDS    .
SOURCE      Klebsiella pneumoniae 30660/NJST258_1
  ORGANISM  Klebsiella pneumoniae 30660/NJST258_1
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 14249)
  AUTHORS   Deleo,F.R., Chen,L., Porcella,S.F., Martens,C.A., Kobayashi,S.D.,
            Porter,A.R., Chavda,K.D., Jacobs,M.R., Mathema,B., Olsen,R.J.,
            Bonomo,R.A., Musser,J.M. and Kreiswirth,B.N.
  TITLE     Molecular dissection of the evolution of carbapenem-resistant
            multilocus sequence type 258 Klebsiella pneumoniae
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 111 (13), 4988-4993 (2014)
   PUBMED   24639510
REFERENCE   2  (bases 1 to 14249)
  AUTHORS   DeLeo,F.R., Chen,L., Porcella,S.F., Martens,C.A., Kobayashi,S.D.,
            Porter,A.R., Chavda,K.D., Jacob,M., Mathema,B., Olsen,R.J.,
            Musser,J.M., Bonomo,R. and Kreiswirth,B.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2014) Laboratory of Human Bacterial Pathogenesis,
            NIAID, Rocky Mountain Laboratories, 903 South 4th St, Hamilton, MT
            59840, USA
FEATURES             Location/Qualifiers
     source          1..14249
                     /organism="Klebsiella pneumoniae 30660/NJST258_1"
                     /mol_type="genomic DNA"
                     /strain="30660/NJST258_1"
                     /isolation_source="urinary tract infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1420012"
                     /plasmid="pNJST258N4"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            1..3006
                     /locus_tag="KPNJ1_05810"
     CDS             1..3006
                     /locus_tag="KPNJ1_05810"
                     /codon_start=1
                     /transl_table=11
                     /product="Transposase"
                     /protein_id="AHM88186.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Transposase"
     gene            complement(3039..3320)
                     /locus_tag="KPNJ1_05791"
     CDS             complement(3039..3320)
                     /locus_tag="KPNJ1_05791"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AHM88187.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCD"
                     /name="hypothetical protein"
     gene            complement(3327..4058)
                     /locus_tag="KPNJ1_05792"
     CDS             complement(3327..4058)
                     /locus_tag="KPNJ1_05792"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /protein_id="AHM88188.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="Hypothetical protein"
     gene            complement(4058..6022)
                     /locus_tag="KPNJ1_05793"
     CDS             complement(4058..6022)
                     /locus_tag="KPNJ1_05793"
                     /codon_start=1
                     /transl_table=11
                     /product="Mobilization protein A"
                     /protein_id="AHM88189.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="Mobilization protein A"
     gene            complement(6035..6310)
                     /locus_tag="KPNJ1_05794"
     CDS             complement(6035..6310)
                     /locus_tag="KPNJ1_05794"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AHM88190.1"
                     /translation="MRVVSRSLWRTLTGAGTPEKTALGCRLFLSHAGACSLFLRGCAFS
                     AWGGKRLASVVKAERNHHGRDRRRHALGHVRGLCSGGCTSRRQTIV"
                     /name="hypothetical protein"
     gene            7078..7257
                     /locus_tag="KPNJ1_05795"
     CDS             7078..7257
                     /locus_tag="KPNJ1_05795"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AHM88191.1"
                     /translation="MALSHSSHSGISARCRSFAPSWAVSKNPPFSPTAAPYPVTVHLSP
                     TRKSTQNATGSSHW"
                     /name="hypothetical protein"
     gene            complement(7503..7652)
                     /locus_tag="KPNJ1_05796"
     CDS             complement(7503..7652)
                     /locus_tag="KPNJ1_05796"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AHM88192.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="hypothetical protein"
     gene            complement(7737..7994)
                     /locus_tag="KPNJ1_05797"
     CDS             complement(7737..7994)
                     /locus_tag="KPNJ1_05797"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AHM88193.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="hypothetical protein"
     gene            complement(8004..9689)
                     /locus_tag="KPNJ1_05798"
     CDS             complement(8004..9689)
                     /locus_tag="KPNJ1_05798"
                     /codon_start=1
                     /transl_table=11
                     /product="Colicin-like bacteriocin, tRNase domain protein"
                     /protein_id="AHM88194.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="Colicin-like bacteriocin, tRNase domain protein"
     gene            9811..10038
                     /locus_tag="KPNJ1_05799"
     CDS             9811..10038
                     /locus_tag="KPNJ1_05799"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AHM88195.1"
                     /translation="MRFCENDQKNSHSHHAQSPPDATKTSTNGAVLRPQYVLLKSLYEI
                     PESPLRDRKRNGGSGSGCSVTEHGQKLMIR"
                     /name="hypothetical protein"
     gene            complement(10017..10196)
                     /locus_tag="KPNJ1_05800"
     CDS             complement(10017..10196)
                     /locus_tag="KPNJ1_05800"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AHM88196.1"
                     /translation="MPGMLFHAALWSTGSESCCRCYHGDKTQFAAGTSHERLLTFNSFF
                     SLSYISAGYLIISF"
                     /name="hypothetical protein"
     gene            10092..10511
                     /locus_tag="KPNJ1_05801"
     CDS             10092..10511
                     /locus_tag="KPNJ1_05801"
                     /codon_start=1
                     /transl_table=11
                     /product="Entry exclusion protein 2 (exc2)"
                     /protein_id="AHM88197.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Entry exclusion protein 2 (exc2)"
     gene            complement(10784..11104)
                     /locus_tag="KPNJ1_05802"
     CDS             complement(10784..11104)
                     /locus_tag="KPNJ1_05802"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AHM88198.1"
                     /translation="MAPCFHSTSYGIAQFLITAMQALAELFRGHTRTRVSTGGGHFGAN
                     LIFILCLGQFFVSQSLQQRSFVRATFRQYAGQAFSQFPKKVGVFVVCSVAHCGASRVNA
                     AI"
                     /name="hypothetical protein"
     gene            11046..11369
                     /locus_tag="KPNJ1_05803"
     CDS             11046..11369
                     /locus_tag="KPNJ1_05803"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AHM88199.1"
                     /translation="MAVIRNCAMPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDR
                     VLAAADKLADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPH
                     IVL"
                     /name="hypothetical protein"
     gene            complement(11431..11808)
                     /locus_tag="KPNJ1_05804"
     CDS             complement(11431..11808)
                     /locus_tag="KPNJ1_05804"
                     /codon_start=1
                     /transl_table=11
                     /product="Entry exclusion protein 1 (exc1)"
                     /protein_id="AHM88200.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="Entry exclusion protein 1 (exc1)"
     gene            11808..12056
                     /locus_tag="KPNJ1_05805"
     CDS             11808..12056
                     /locus_tag="KPNJ1_05805"
                     /codon_start=1
                     /transl_table=11
                     /product="Regulatory protein ROP"
                     /protein_id="AHM88201.1"
                     /translation="MSHPAIFETVYLTGAHHARKERIMTKQEKTALNMAKFIKEQSLLL
                     LDKLNELDMDTPADMCERLHEDAETLWQVMRDKLGEE"
                     /name="Regulatory protein ROP"
     gene            complement(12084..12320)
                     /locus_tag="KPNJ1_05806"
     CDS             complement(12084..12320)
                     /locus_tag="KPNJ1_05806"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AHM88202.1"
                     /translation="MERFAIENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKA
                     ELMREYNSRYFNVPVPELPEDKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            12354..12446
                     /locus_tag="KPNJ1_05807"
     CDS             12354..12446
                     /locus_tag="KPNJ1_05807"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AHM88203.1"
                     /translation="MERKLTLRDFGHEIIKKDLHLDPFKLKMKF"
                     /name="hypothetical protein"
     gene            complement(12486..13346)
                     /locus_tag="KPNJ1_05808"
     CDS             complement(12486..13346)
                     /locus_tag="KPNJ1_05808"
                     /EC_number="3.5.2.6"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-lactamase"
                     /protein_id="AHM88204.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="Beta-lactamase"
     gene            complement(13529..14086)
                     /locus_tag="KPNJ1_05809"
     CDS             complement(13529..14086)
                     /locus_tag="KPNJ1_05809"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AHM88205.1"
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="hypothetical protein"
     ARG             complement(12490..13346)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     BGC             3004..14249
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6822..7400
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3331..4058)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.34e-150"
ORIGIN
        1 atgcccgttg actttctgac cactgagcag actgaaagct atggcagatt caccggtgaa
       61 ccggatgagc ttcagctggc acgatatttt caccttgatg aagcagacaa ggaatttatc
      121 ggaaaaagca gaggtgatca caaccgtctg ggcattgccc tgcaaattgg atgtgtccgt
      181 tttctgggca ccttcctcac cgatatgaat catattcctt ccggcgtccg gcattttacc
      241 gccagacagc tcgggattcg tgatatcacc gttcttgcag aatacggtca gagggaaaat
      301 acccgccgtg agcatgcagc gctgatacgt cagcactatc agtatcgtga atttgcctgg
      361 ccctggacat ttcgccttac ccgtctttta tatacccgga gctggataag caacgaacgt
      421 cctggcctgc ttttcgatct ggcgacaggg tggcttatgc aacatcgtat tattctcccc
      481 ggagccacta cgctgacccg gttgatttca gaggtaaggg aaaaggcgac gttgcgcctg
      541 tggaacaaac tggcactgat accgtcagcc gaacagcgtt cacagctgga gatgctgctg
      601 gggccaactg attgcagccg cctgtcttta ctggaatcac tgaaaaaggg ccctgtgacc
      661 atcagtggtc cggcgtttaa tgaagcaatt gaacgctgga aaactctgaa cgattttggc
      721 ctgcatgctg aaaacctgag tacactcccg gctgtgcgcc tgaaaaatct cgcacgttat
      781 gctggtatga cttcggtgtt caatattgcc aggatgtcac cgcagaaaag gatggcggtt
      841 ctggttgcct ttgtccttgc atgggaaacg ctggcgctgg atgatgcatt ggacgttctg
      901 gacgccatgc tggccgttat catccgtgac gccagaaaga ttgggcagaa aaaacggctc
      961 cgctcgctga aggatctgga taaatctgca ttggcgctcg ccagcgcatg ttcgtacctg
     1021 ctgaaagaag aaacaccgga cgaatcgatt cgtgctgagg tgttcagcta catcccaagg
     1081 caaaagctgg ctgaaatcat cacgcttgtc cgtgaaattg cccggccctc agacgataat
     1141 tttcatgaag aaatggtgga gcagtacggg cgcgttcgtc gtttcctgcc ccatctgctg
     1201 aataccgtta aattttcatc cgcacctgcc ggggttacca ctctgaatgc ctgtgactac
     1261 ctcagccggg agttcagctc acggcggcag ttttttgacg acgcaccaac ggaaattatc
     1321 agtcggtcat ggaaacggct ggtgattaac aaggaaaaac atatcacccg caggggatac
     1381 acgctctgct ttctcagtaa actgcaggat agtctgaggc ggagggatgt ctacgttacc
     1441 ggcagtaacc ggtggggaga tcctcgtgca agattactac agggtgctga ctggcaggca
     1501 aaccggatta aggtttatcg ttctttgggg cacccgacag acccgcagga agcaataaaa
     1561 tctctgggcc atcagcttga tagtcgttac agacaggttg ctgcacgtct tggcgaaaat
     1621 gaggctgtcg aactcgatgt ttctggcccg aagccccggt tgacaatttc tcccctcgcc
     1681 agtcttgatg agccggacag tctgaaacga ctgagcaaaa tgatcagtga tctgctccct
     1741 ccggtggatt taacggagtt gctgctcgaa attaacgccc ataccggatt tgctgatgag
     1801 tttttccatg ccagtgaagc cagtgccaga gttgatgatc tgcccgtcag catcagcgcc
     1861 gtgctgatgg ctgaagcctg caatatcggt ctggaaccac tgatcagatc aaatgttcct
     1921 gcactgaccc gacaccggct gaactggaca aaagcgaact atctgcgggc tgaaactatc
     1981 accagcgcta atgccagact ggttgatttt caggcaacgc tgccactggc acagatatgg
     2041 ggtggaggag aagtggcatc tgcagatgga atgcgctttg ttacgccagt cagaacaatc
     2101 aatgccggac cgaaccgcaa atactttggt aataacagag ggatcacctg gtacaacttt
     2161 gtgtccgatc agtattccgg ctttcatggc atcgttatac cggggacgct gagggactct
     2221 atctttgtgc tggaaggcct tctggaacag gagaccgggc tgaatccaac cgaaattatg
     2281 accgatacgg caggtgccag cgatcttgtc tttggccttt tctggctgct gggataccag
     2341 ttttctccac gcctggctga tgccggtgct tcggttttct ggcgaatgga ccatgatgcc
     2401 gactatggcg tgctgaatga tattgccaga gggcaatcag atccccgaaa aatagtcctt
     2461 cagtgggacg aaatgatccg gaccgcaggc tccctgaagc tgggcaaagt acaggcctca
     2521 gtgctggtcc gttcattgct gaaaagtgaa cgtccctccg gactgactca ggcaatcatt
     2581 gaagtggggc gcatcaacaa aacgctgtat ctgcttaatt atattgatga tgaagattac
     2641 cgccggcgca ttctgaccca gcttaatcgg ggagaaagcc gtcatgcagt tgccagagcc
     2701 atctgtcacg gtcaaaaagg tgagataaga aaacgatata ccgacggtca ggaagatcag
     2761 ttgggagctc tggggctggt cactaacgcc gtcgtgttat ggaacactat ttatatgcag
     2821 gcagctctgg atcatctccg ggcgcagggt gaaacactga atgatgaaga tatcgcacgc
     2881 ctctccccgc tttgccacgg acatatcaat atgctcggcc attattcctt cacgctggca
     2941 gaactggtga ccaaaggaca tctgagacca ttaaaagagg cgtcagaggc agaaaacgtt
     3001 gcttaacgtg agttttcgtt ccactgagcg tcagacccct aatcacacag cttcattcct
     3061 gtgggtttgt tccgttccca ttgcggattt ttaccagtga aaaagtacgt ggctttttcg
     3121 tcgctgttga gccgctcgtc atacatcccg atggatttca tcatcgccag atgctgggcc
     3181 cggtcgacaa aacagaaaac atacgccccg gtctgccagg cgagcaccac catatcttcc
     3241 agatgctcgc tggtcggcat gatgcagcgc tcgccgttcc ggtcctccgc gtcttcaata
     3301 aataccatgt acagcgccat aatccctcac tccgttgtca taaatttaaa gcacgcaaac
     3361 aaccgttcac gatccagctc tgccgttttg cgggcctcgc cggtctgtgg gttcacgatt
     3421 tcatccaccg acaggattgc ccgctgcacc ctgacctgcg tcgcccggtc cgggcagatg
     3481 taccagattt catcgatgcc gttagctttg cggttgaaca gatacccgga caccacttcc
     3541 tgataccggc gacggctctt gacggtgcgc tccacctcaa acgccaccgt tttaccgtcc
     3601 agctccgcca gggcgtccgg cactttcatg cctttaagac tcagacggtg cagaatgcgc
     3661 cagccggtgg cccctctggc ttccatatca aggcgcaggg attgcacggc cagccggtgc
     3721 ggcagatgtg ctgccgacac ccttgacggc tgaaacggaa taacatccgc cagcggttcg
     3781 tcctcgtcga acgagaacgc cacgccatgc ggcgtcagcc cccacacgct gacctgacgg
     3841 ccgtccaccg gatatttggt ccggaccagc agccctttcg ccaccaggct gtcgaggaag
     3901 cggtagaccg gctgcaccgc cgcaatcccc agccgccgcg caaccagctc cgccgtggaa
     3961 cacagctctt cccgcaacca gcgcagggtc agccgggttt tctccgcgtt ccgcgcctgt
     4021 cgtttcgcgt ggctgacgtt gtacagctcc agcgccatca gtaaatcccc gcaaaatcat
     4081 catccgcttt cggcccgccg ccgtcggccg ggggccggaa ctccgccagg ggatcggttt
     4141 tcgccagcac cgggaaacgc tgcaacggga tttcccggcg ctcaacccgc agcggactgc
     4201 tgaacgccat ccgggccaca cccagcccgg tcatgaccgc gaacccgtcc ggcaggtgtt
     4261 gcagggtgtt cacgtcaaac agcggccgcg cctccttctg gatgaagctg tccccgctga
     4321 tatgctccac gttccccgcc tcccggctgg cacggcggcg ctccacgtcc accctgattt
     4381 ccccggtctg ccccgccgcc cactgcgcgg actccaggct ggccgcccgg taaaaccagc
     4441 ggatcggcgt gttatccagt accgtggttt tcacaaattc agcgggcaga tcctgcccgc
     4501 actgcccgaa atcgcccagc gactggtgcg cgagcaacag attgcagtcc cggctgcgca
     4561 gcgtccccag tgcgttcagt acatacttcg acagcagata tttaatttca tcgagcatca
     4621 cgctgacgtg tggccactcc cggaactcgt cccgcgcaat gatgatttgc gcgcagcggg
     4681 caaacagcat tttttgcaca cggatcaccg cctcgtcgtc catcgagccg ataacgtaca
     4741 ggcagccgcc gccgttgata atccccgcca catcgatgcc actgtccgtc tgcaggacgc
     4801 tcaggtcagc gacgttttcc agcctagtga tcaacccctt cacgtccttt ttcagggcct
     4861 ccggcagcgc gtacgccgcc gccagcacct gctgaatgtt tgccccctgc ggaaactgct
     4921 ccgcaatcag cttcgccgct ttctgctcgc tgatgcggta gtggtccgcc acgtcgccgg
     4981 tctcgcccag gttaagcccg gcaatcagca ggtttttcag cgcgtactgg tcgagatcgc
     5041 ggaacagatt cagctgcgcc ggtttccccg gccgcagatc cagcagggtg aagtccggac
     5101 agtgcgcccg gaagacgtgc ggcgcccatg cgtccccttt ggggtcgaag tacaccaccg
     5161 cgtccccgaa atcgcgtacg cagcgaatga gcgcgttggt ggccatcacg ctcttgccgc
     5221 tcccgggcag gccgaggatc tgaatgttgg tcttgcgcca ggtatcacgc ggaaccagca
     5281 ccggcttgcc ctgctcatcc ttacccagaa acaccgcctg ctcctgccgg gcctgctccg
     5341 aggaaaaatc ggccagcgca ttaaccggta ccggctcccc gaaatgcagg tggcgcacgt
     5401 cggtcatctc tcctgtccgc tggctgctgc gccggctctc ctgattcagt ttcgcgttca
     5461 ccgcctcgtc agcagccgca aaatgaccgt caagcagcag gaacgccagc agccgcgcca
     5521 acacaatccc gacagccagc ccgcccgcgc accaggcaat cccgaacagg ttagccggct
     5581 gcagcaggta ataatctgcg tttgcgtaga ggtccggacg caggacggtt tctccccagc
     5641 tctcgataaa cacggcgaca accagccccc ccagaagggc cagaccggcc gcgccggtca
     5701 gccagtacgc gtagttccag acatgtcgac gtgccctgac catccgcccg ggcgaatcca
     5761 gcgaggccag caccgaaaat ggcgctggcc gtttattatc gcgcttatag cgatcggcat
     5821 actcccgtcg ctccttatga ctcagcccca caaaacgcat ccgtccggcc caaagttcag
     5881 ccagaccgaa aaacaggccg ataactagga aagccccggc cagaccgtaa tactcctggt
     5941 acagccagct gacccacgga taaatctgcc gggccagctc cggaatgtgc cacttccagg
     6001 caagcgaatc cgtattaaac atgttctctc tccttcagac gatggtttgc cggcgtgatg
     6061 tgcagccccc gctgcacaag ccgcggacat gccccaaggc atggcggcgg cggtcacgtc
     6121 cgtggtgatt gcgttcagct ttcaccacgg aggccagcct ctttccgccc catgcggaaa
     6181 aggcgcagcc ccggaggaac aacgagcacg ccccagcgtg tgagaggaaa aggcggcacc
     6241 ccagtgcggt cttttccggt gttccggccc ccgtcagagt tcgccatagc gagcggctga
     6301 ccacccgcaa aatcaggagc agctttgctg cttcgccagg tgcgtccagc accgtggatt
     6361 tttgtgggtg gtcaggcgtg agcctgacgg gggccgggtg aatttctggc gtggccggcg
     6421 tccagccgga cggagccaga aagaggggga gagaccgtgc gtcggtggcg tccagccagc
     6481 gtaagcaggg gatatccccc tgaggtcagc cacgccctcg cggggatgac ctgtatttct
     6541 ccgtaaggag aaaaaagcaa cagagatagg ttaacgtaaa atgttcagcg aaaaaccgat
     6601 atgattcacc tatcctggta atttattatc agggaatata aagacttaat caggggacaa
     6661 tatgccacat cagcgcgtcc ggtgtccgtc ttgtaggtgc cggggaaatt gcgcctcctc
     6721 gctcactcgg tcgctacgct ccggccgtga gactgcggcg ggcgctgcgg acacatgcaa
     6781 agttacccac agattccgtg aataagcagg ggactaacat gtgaggcaaa acggcaaggc
     6841 cacgcaggtg gcgtttttcc ataggctccg ccctcctgac tgagttcaca taaacagacg
     6901 ctttttcggt gcatctgtgg gagccggtgg ctcaaccatg aatcagtcag gagtggcgaa
     6961 acccgacagg acttaaagat catcaccgtt tccgatgaat cgctccctct tgcgctctcc
     7021 tgttccaacc ctgccgtttc ccggatacct gttccgcctt tctcccttgc gggaagtgtg
     7081 gcgctttctc atagctcaca ctctggtatc tcggctcggt gtaggtcgtt cgctccaagc
     7141 tgggctgtaa gcaagaaccc cccgttcagc ccgactgctg cgccttatcc ggtaactgtt
     7201 cacttgagtc caacccggaa aagcacgcaa aacgccactg gcagcagcca ttggtaactg
     7261 ggagttcgca gaggatttgt ttagctaaac acgcggttgc tcttgaagtg tgcgccaaag
     7321 tccggctaca ctggaaggac agatttggtt gctgtgctct gcgaaagcca gttaccacgg
     7381 ttaagcagtt ccccaactga cttaaccttc gatcaaacca cctccccagg tggttttttc
     7441 gtttacaggg caaaagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7501 ttctactgaa ccgcgatccc cgtcagttca gaggaggacg atggtgccac cgttccaccc
     7561 tgaacatccc ggatatagtt tgcctgacat gcgaccagaa gaaagcctga aaaaatcaat
     7621 ataaaaagaa aaacaacctt tacctttttc atataccttc cctgtattta aaaaccccgg
     7681 tattcatccc tgaacaccgg aagtctgtat ttcaaatcat ccttgataac ttttgcttac
     7741 cagttaccat cccggtaaac aaaggataca aagtaatcaa atttactaat atcgattaca
     7801 tttttaaagt gtggctgtaa tatcgaaacc catgattttt caacatcaaa ccaaccatta
     7861 ttaatattat cctttaaagg catccccaga ctttcaatga cagaaccatc atcaccgaag
     7921 tcttttgagt attcaccacc tttaaactct tcggttttct tatcaaacca atgaatatgt
     7981 aatttaagcc ccatatttac ctcttaaaga tattttttaa tgtttcgttt tggatccggc
     8041 cctttaacct gcttacccgt ttttggatcg aatgccccga ggtgttcgcc atcactggcg
     8101 cggtaccctt caagctcacc gtgctgggaa tcccattcat aaattttacg ctttttatca
     8161 ccataccatc gggcccgctt accaccacca ccttgtttag gggttttagg tttaccctct
     8221 ttcaactctc ccaacccctt aatatcttca gtcttgggat caggaaaata atcatggccg
     8281 taatctttag ttcccttacg aggttttttc ttttcctcat ttaatttatt ttctgcatcc
     8341 ttcgcttttt tctccttttg tttccggctc tccatcgccc cggaaagcgc agccttatcc
     8401 ctggataagg attcattcat ttcgtttaaa gatgtctggg cagcttttaa tttctcattc
     8461 tgattctgaa tatccttatc aagatagcga acctgatcct gccacccgga gccatattca
     8521 tgaggcgggt tataagtcac aaaatcatct ttatttgcct ctgcctcttt taccttttta
     8581 tcggcttcct gaacagcagc ccttgcaccg gggatcgcct tctctgtcga tgcaatgcga
     8641 ctgtttaagg tcgcaatatt tttatcttca gcatccagct cagctttcgc tttatcatac
     8701 tctcttttca gcccctcttc cgggtgagca gcgtcccatg cctgctggcg acgcttttct
     8761 tcctccagac gctgttttac ctgtgccgga gtaagaacat ccgtcaccga cacatagacc
     8821 ggtttctgtc cgctctcttt cgggaaacga ataacggctt cacgggagtt accaccggcc
     8881 gtaaaaccag ccggacgtga atcacctttt tcagcaataa tgccttttgg cggggctttc
     8941 gcggccggaa cacctttagg tacgctcacc tgcagagacg ggagacccgg aatcgacaca
     9001 ctgaataccc ccggacgttt tgtcggtttc gcatccacca caggaacact cattggccgg
     9061 ccggtgacaa ccgcaatatg ctgccgctcg tccttcacaa tatccgccac acgtttagtg
     9121 acgctgaccg tcgcctggtc cagcggcagg gagctgaccg gcgtctccgt taccgtgtct
     9181 gccggcaatg acgtcacgat ttttgacatc attttcggat catcttttgc tatctcagaa
     9241 ggcagcactc cgtaaatcgc aatcccccac agaccaaact taaacggacc tttcagggca
     9301 gccagcacat cagcaacagc ggccgacaac gcatcgccgg aaacggataa agccagtcct
     9361 tcagcccccg gagtggccaa ggcaggcaga ccgaaggcca tcgcggtggc cgaagactgt
     9421 ccgccaccag agctcccgct atgattgccg ctgttaccgc cattaccacc gggtttactc
     9481 ccgccgttac caaactcccc cgggttatag ttatgaatat ccccgttagg tgtatgcgtc
     9541 gtaccccagc ccggcccgct gtttggatcc gtaccaccac ctgatgaagg gccaccttta
     9601 cctgatgtac cgttcacatt cccactggcc tgaccaccat tgtgtcccag acctgaatta
     9661 cccggacctc gtccgtctcc gccactcata tacttttcct ctttgacttt taaaacaata
     9721 aactaaaata aaatactgta catataacca ctggttttat atacagtata aaaattacgc
     9781 tgccttgttt ttcatgtcaa gtctgtaaat ttgcgttttt gtgaaaatga tcaaaaaaac
     9841 agccactcac accatgcgca atcccctcca gatgcaacaa aaaccagcac aaacggcgcc
     9901 gttctccggc ctcagtatgt tttactgaaa tcattatatg agataccaga aagccccctc
     9961 cgggacagga aaagaaacgg ggggtctggt tcgggttgtt cggtcactga acacggtcag
    10021 aaactgatga taagatagcc tgcggatata taactcaatg agaaaaaaga attaaaggta
    10081 aggagacgct catgagacgt tccggcagca aactgtgtct tatcgccatg ataacagcga
    10141 cagcaactct cactgcctgt gctccaaagg gcagcgtgga acagcatacc cggcattacg
    10201 tatatgcttc agatgacggt tttgatccca atttttccac ccagaaagct gacacaacac
    10261 gaatgatggt gccttttttc cggcagttct gggatatggg cgcaaaggat aaagcgaccg
    10321 gaaaatcacg gagtgatgtg cagcaacgca ttcagcagtt tcacagtcag gaatttttaa
    10381 actcactcca aggcacaaca caatttgcgg gtaccgatta ccgcagcaaa gacctcacac
    10441 cgaaaaaatc aaggctactg gctgacacca tttcagctgt ttatctcgat ggctatgaag
    10501 gcagacagta agtggattta ccataacgcg tcattacgcg caccgctcag acgggctcag
    10561 ggcgtttccg gcggctacgg gataaaacag gcaacaaccc ccccggaaaa ctgccggaaa
    10621 cgcgcccgat aaattttatc ctaatgcatg cctatgcacg caactgcatg gcatatgttt
    10681 tgacgtaaaa tggatgccgt aagccctttt ttaccgactt tctgcttcat cagcctgact
    10741 gacacgacca tattctttca gaccgccccc tgctaaactt ccttcatatt gccgcattaa
    10801 cccgggaggc tccgcaatga gcaacactac acactacgaa aacgccaact ttcttcggga
    10861 actggctgaa agcctgcccc gcatactgcc ggaaggtggc ccggacaaag ctgcgctgtt
    10921 gcagcgactg gctaacgaag aactggccca ggcagagtat gaagatcagg ttcgcgccaa
    10981 agtgaccgcc gcccgtgctg acacgcgtcc gggtatgacc acggaacaac tccgccagcg
    11041 cctgcatggc cgttatcagg aactgcgcga tgccgtatga cgtggaatgg aagcagggag
    11101 ccatcgagga tgtaacgacg ctgtttgact atatcgcgga aaattcatcc ctgtgggatg
    11161 cgcagaacgt taccgaccgg gttctcgctg cggcggacaa actggccgac ttcccgcgac
    11221 tctatgaagt ggactcacgc tatggcgagg acgttcgccg gatcagtctg atgggacagc
    11281 atgtcctcta tgatgttgat gaccagacca gaaaagtccg ggttctggcc gtggtcggcc
    11341 agcgccagaa tccccatatt gttctctaat aaccggtgac ggggacgcaa ttcattgcgt
    11401 ggccgtcgcc ggttggacaa gcacagcgcg tcagcgtttt ccccacggcc accaccggcg
    11461 cggtgttccg gccggggtct ctgctgtctc cggtttgtgt tccagcagtc tcatggactg
    11521 catttcctgc cgtagcccgg ccacctcctc cctgagtgct tttatctccg cgatcaacgc
    11581 gtcggaggcc attttttcag tctcacccga cagtctcaac tcgtcccgct cgtgagtctc
    11641 atcctgtctt aactcaccgt acacccggat aagctcgctg gtatccacca cccggccgcc
    11701 atcagcttcc gtacggtatg atacgcggcc tttcgccata tcccgataca gggaacttcg
    11761 ggctttaccc gtcagttccg tcgcctgtct caccgtcacc cacgccattg tctcaccccg
    11821 ctatttttga gacagtatac ctgacaggcg cacaccatgc ccggaaggaa agaatcatga
    11881 caaaacagga aaaaacagcc ctcaatatgg ctaaatttat taaggagcag tcgctgctat
    11941 tgctcgataa actgaatgag ctggatatgg ataccccggc cgacatgtgc gagcggctac
    12001 acgaggatgc ggaaacactc tggcaggtga tgcgggataa gctcggggag gaataacagc
    12061 accggcatgg ccggtgctgg catttatttt ttgccgaacg gccaccaccg gcgggcaggt
    12121 ttgtcctccg gcagttcagg taccggcacg ttaaaatagc ggctgttata ttcccgcatc
    12181 agctccgctt tcagcttctc gttcaccgcg ctttccccgt gccgctcact caccacgtca
    12241 atcatcgcgt taatctgctc cggtgagcac ttcccggcat caaccacctg cacaccgttc
    12301 tcgatggcga accgctccac cttgcgatag aggtaatcgg ggtctgacgc tcagtggaac
    12361 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    12421 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    12481 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    12541 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    12601 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    12661 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    12721 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    12781 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    12841 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    12901 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    12961 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    13021 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    13081 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
    13141 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    13201 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    13261 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    13321 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    13381 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    13441 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    13501 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattttat
    13561 aaaccgtgga gcgggcaata ctgagctgat gagcaatttc cgttgcacca gtgcccttct
    13621 gatgaagcgt cagcacgacg ttcctgtcca cggtacgcct gcggccaaat ttgattcctt
    13681 tcagctttgc ttcctgtcgg ccctcattcg tgcgctctag gatcctccgg cgttcagcct
    13741 gtgccacagc cgacaggatg gtgaccacca tttgccccat atcaccgtcg gtactgatcc
    13801 cgtcgtcaat aaaccgaacc gctacaccct gagcatcaaa ctcttttatc agttggatca
    13861 tgtcggcggt gtcgcggcca agacggtcga gcttcttcac cagaatgaca tcaccttcct
    13921 ccaccttcat cctcagcaaa tccagccctt cccgatctgt tgaactgccg gatgccttgt
    13981 cggtaaagat gcggttagct tttacccctg catctttgag cgctctgatc tgaatatcga
    14041 gggactgctg gctggttgag acccgcgcat aaccaaaaat tcgcataaaa atgtacctta
    14101 aatcgaatat cagacacgat gtgtctatta tgccaaaatg acgatttaat ggacactcaa
    14161 acgaagccgt tttactatgt ctgataattt ataacatttc gtacggttgg aaaaatgtta
    14221 ctaaatgccc gtcaggcagg gaggccgat
//
