LOCUS       CP006924               10925 bp    DNA     circular BCT 26-JUL-2016
DEFINITION  Klebsiella pneumoniae 30660/NJST258_1 plasmid pNJST258N5, complete
            sequence.
ACCESSION   CP006924
VERSION     CP006924.1
DBLINK      BioProject: PRJNA226698
            BioSample: SAMN03081502
KEYWORDS    .
SOURCE      Klebsiella pneumoniae 30660/NJST258_1
  ORGANISM  Klebsiella pneumoniae 30660/NJST258_1
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Deleo,F.R., Chen,L., Porcella,S.F., Martens,C.A., Kobayashi,S.D.,
            Porter,A.R., Chavda,K.D., Jacobs,M.R., Mathema,B., Olsen,R.J.,
            Bonomo,R.A., Musser,J.M. and Kreiswirth,B.N.
  TITLE     Molecular dissection of the evolution of carbapenem-resistant
            multilocus sequence type 258 Klebsiella pneumoniae
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 111 (13), 4988-4993 (2014)
   PUBMED   24639510
REFERENCE   2  (bases 1 to 10925)
  AUTHORS   DeLeo,F.R., Chen,L., Porcella,S.F., Martens,C.A., Kobayashi,S.D.,
            Porter,A.R., Chavda,K.D., Jacob,M., Mathema,B., Olsen,R.J.,
            Musser,J.M., Bonomo,R. and Kreiswirth,B.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2014) Laboratory of Human Bacterial Pathogenesis,
            NIAID, Rocky Mountain Laboratories, 903 South 4th St, Hamilton, MT
            59840, USA
FEATURES             Location/Qualifiers
     source          1..10925
                     /organism="Klebsiella pneumoniae 30660/NJST258_1"
                     /mol_type="genomic DNA"
                     /strain="30660/NJST258_1"
                     /isolation_source="urinary tract infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1420012"
                     /plasmid="pNJST258N5"
                     /geo_loc_name="USA"
                     /collection_date="2010"
     gene            1..192
                     /locus_tag="KPNJ1_05811"
     CDS             1..192
                     /locus_tag="KPNJ1_05811"
                     /codon_start=1
                     /transl_table=11
                     /product="Regulatory protein ROP"
                     /protein_id="AHM87882.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDSLQDGN"
                     /name="Regulatory protein ROP"
     gene            192..614
                     /locus_tag="KPNJ1_05812"
     CDS             192..614
                     /locus_tag="KPNJ1_05812"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AHM87883.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     VSELIRVFGSLDGVTDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPSHSPWWKFWKKS"
                     /name="hypothetical protein"
     gene            828..920
                     /locus_tag="KPNJ1_05813"
     CDS             828..920
                     /locus_tag="KPNJ1_05813"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AHM87884.1"
                     /translation="MERKLTLRDFGHEIIKKDLHLDPFKLKMKF"
                     /name="hypothetical protein"
     gene            complement(960..1817)
                     /locus_tag="KPNJ1_05814"
     CDS             complement(960..1817)
                     /locus_tag="KPNJ1_05814"
                     /EC_number="3.5.2.6"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-lactamase"
                     /protein_id="AHM87885.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRG
                     TELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSAL
                     PAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASL
                     IKHW"
                     /name="Beta-lactamase"
     gene            complement(2000..2251)
                     /locus_tag="KPNJ1_05815"
     CDS             complement(2000..2251)
                     /locus_tag="KPNJ1_05815"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AHM87886.1"
                     /translation="MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVD
                     RNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
                     /name="hypothetical protein"
     gene            complement(2517..3356)
                     /locus_tag="KPNJ1_05816"
     CDS             complement(2517..3356)
                     /locus_tag="KPNJ1_05816"
                     /EC_number="3.5.2.6"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-lactamase"
                     /protein_id="AHM87887.1"
                     /translation="MKDTLMKKILLLHMLVFVSATLPISSVASDEVETLKCTIIADAIT
                     GNTLYETGECARRVSPCSSFKLPLAIMGFDSGILQSPKSPTWELKPEYNPSPRDRTYKQ
                     VYPALWQSDSVVWFSQQLTSRLGVDRFTEYVKKFEYGNQDVSGDSGKHNGLTQSWLMSS
                     LTISPKEQIQFLLRFVAHKLPVSEAAYDMAYATIPQYQAAEGWAVHGKSGSGWLRDNNG
                     KINESRPQGWFVGWAEKNGRQVVFARLEIGKEKSDIPGGSKAREDILVELPVLMGNK"
                     /name="Beta-lactamase"
     gene            complement(3401..4189)
                     /locus_tag="KPNJ1_05817"
     CDS             complement(3401..4189)
                     /locus_tag="KPNJ1_05817"
                     /EC_number="2.7.7.47"
                     /codon_start=1
                     /transl_table=11
                     /product="Streptomycin 3''-adenylyltransferase"
                     /protein_id="AHM87888.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /name="Streptomycin 3''-adenylyltransferase"
     gene            complement(4259..4864)
                     /locus_tag="KPNJ1_05818"
     CDS             complement(4259..4864)
                     /locus_tag="KPNJ1_05818"
                     /EC_number="2.3.1.82"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside N6'-acetyltransferase"
                     /protein_id="AHM87889.1"
                     /translation="MSIQHFQTKLGITKYSIVTNSNDSVTLRLMTEHDLAMLYEWLNRS
                     HIVEWWGGEEARPTLADVQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGW
                     WEEETDPGVRGIDQXLANASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAI
                     RCYEKAGFERQGTVTTPDGPAVYMVQTRQAFERTRSVA"
                     /name="Aminoglycoside N6'-acetyltransferase"
     gene            complement(5047..5604)
                     /locus_tag="KPNJ1_05819"
     CDS             complement(5047..5604)
                     /locus_tag="KPNJ1_05819"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AHM87890.1"
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="hypothetical protein"
     gene            5768..8773
                     /locus_tag="KPNJ1_05820"
     CDS             5768..8773
                     /locus_tag="KPNJ1_05820"
                     /codon_start=1
                     /transl_table=11
                     /product="Transposase"
                     /protein_id="AHM87891.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Transposase"
     gene            complement(8959..9201)
                     /locus_tag="KPNJ1_05821"
     CDS             complement(8959..9201)
                     /locus_tag="KPNJ1_05821"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AHM87892.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(10367..10510)
                     /locus_tag="KPNJ1_05822"
     CDS             complement(10367..10510)
                     /locus_tag="KPNJ1_05822"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AHM87893.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDLSPLAAATGNGFAEQRSV
                     EV"
                     /name="hypothetical protein"
     gene            complement(10535..10906)
                     /locus_tag="KPNJ1_05823"
     CDS             complement(10535..10906)
                     /locus_tag="KPNJ1_05823"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AHM87894.1"
                     /translation="MLSVKPVSFPLPRSLSRYAPVVRLRRAAGACIEKLEKTERSKEEK
                     KERPRRCRFSIGSAPLTSIAETDAQISGGETRQDYKDTRRFPLAAPSCALLFLPFGLPV
                     SLRCYGRVYLIPRLTLSSG"
                     /name="hypothetical protein"
     ARG             complement(3405..4189)
                     /name="aadA1"
                     /gene="aadA1"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(4263..4813)
                     /name="aac(6')-Ib'"
                     /gene="aac(6')-Ib'"
                     /gene_name="aminoglycoside N-acetyltransferase AAC(6')-Ib'"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.46"
                     /coverage="100.0"
     ARG             complement(4263..4813)
                     /name="aac(6')-Ib"
                     /gene="aac(6')-Ib"
                     /gene_name="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.46"
                     /coverage="100.0"
     ARG             complement(964..1817)
                     /name="blaTEM"
                     /gene="blaTEM"
                     /gene_name="TEM family class A beta-lactamase"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.95"
                     /coverage="100.0"
     ARG             complement(2521..3341)
                     /name="blaOXA-9"
                     /gene="blaOXA-9"
                     /gene_name="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-9"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(4260..4864)
                     /name="AAC(6')-Ib9"
                     /gene="AAC(6')-Ib9"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="98.46"
                     /coverage="99.01"
     ARG             complement(961..1817)
                     /name="TEM-169"
                     /gene="TEM-169"
                     /gene_name="TEM beta-lactamase"
                     /tool="rgi"
                     /drug_class="monobactam; cephalosporin; penam; penem"
                     /identity="98.95"
                     /coverage="99.65"
     ARG             complement(3402..4177)
                     /name="aadA"
                     /gene="aadA"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.23"
                     /coverage="98.1"
     Typing          complement(10403..10768)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.095"
                     /coverage="85.0"
                     /evalue="1.36e-162"
ORIGIN
        1 atgacgaaac aacagaaaac ggcgctgaac atggctaaat tcatccagaa tcagtctctg
       61 ctcctgctgg agaagcttaa cgagcttgat cttgatgctg aggccgacct ttgtgagaaa
      121 ctccacgatg atgcagagca tcttttccgt acactctcct cgagactgga ttcattacag
      181 gatggtaact gatggccaga gtgtctatta gtgaagctgc acggcttgtt aaggttagtc
      241 gtcccactat ttacaagatg attaacagcg ggaagttaag ttatacctct gttgtaaagc
      301 acgggaagga tattaaagtt attgatgtat ccgagcttat tagggtgttt ggttctcttg
      361 atggtgtaac tgatactgta aaatatgatg taaaaagtga cgcagaatct acaggtatta
      421 acagcgttgg tttacatgat ttacaacata gaatcgcttt acttgaagct gaaaacgatg
      481 gtcttaaagg ggctgttaag gctcgagatg agcatattga ttcccttcgt caggctatgc
      541 agcttctgga gcacaagcac gaaccttctt caccatccca ttccccgtgg tggaaattct
      601 ggaaaaaatc ctgatcctgc catgcttgag ttttcctctc cggccatccc ggagagtttc
      661 tgaaccttcc caaaaatcgc cagatctctg aacgctcctc accactaaaa atacccacac
      721 cccacgcaaa attaaaattt tgcagattca ttatttacaa caatgggtta atgaaaaata
      781 aatgtgcggt gatgtgttac aaagtgcaga tcggggtctg acgctcagtg gaacgaaaac
      841 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      901 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
      961 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1021 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1081 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     1141 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1201 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     1261 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     1321 agctcggttc cacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1381 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1441 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1501 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     1561 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     1621 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     1681 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     1741 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     1801 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     1861 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     1921 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     1981 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaatttta taaaccgtgg
     2041 agcgggcaat actgagctga tgagcaattt ccgttgcacc agtgcccttc tgatgaagcg
     2101 tcagcacgac gttcctgtcc acggtacgcc tgcggccaaa tttgattcct ttcagctttg
     2161 cttcctgtcg gccctcattc gtgcgctcta ggatcctccg gcgttcagcc tgtgccacag
     2221 ccgacaggat ggtgaccacc atttgcccca tatcaccgtc ggtactgatc ccgtcgtcaa
     2281 taaaccgaac cgctacaccc tgagcatcaa actcttttca tgatatatct cccaatttgt
     2341 gtagggctta ttatgcacgc ttaaaaataa taaaagcaga cttgacctga tagtttggct
     2401 gtgagcaatt atgtgcttag tgcatctaac gttcaaggtg agcggactcg cgcggcttta
     2461 tgcgcgaggt ccgctcgagc gcagggttat gcatctgctc tcgatgacgc cacatatcat
     2521 ttgttaccca tcaacacggg taattccacg agaatatcct ctcgtgcttt agacccgccg
     2581 ggaatatcgg acttttcctt tcctatttcc aatcgggcga aaacaacttg ccgtccgttt
     2641 ttttcagccc agcccacgaa ccagccctgc ggacgacttt catttatctt gccgttattg
     2701 tcccgaagcc agccgctgcc gctttttcca tgtacagccc atccttcggc tgcctggtac
     2761 tgcgggattg tggcatacgc catgtcataa gccgcttcgg atacaggcag cttatgcgcg
     2821 acaaagcgta gaagaaactg aatttgctcc ttgggagata tggtgagcga cgacatcagc
     2881 catgactggg tcaagccgtt atgcttcccc gagtcaccgg aaacatcttg attaccgtac
     2941 tcaaatttct ttacgtattc cgtgaaccga tcaactccca gacggcttgt taattgctgc
     3001 gagaaccaga caacagagtc gctttgccat agcgccggat agacttgttt gtatgtgcga
     3061 tctctcggag acgggttgta ttccggcttc aattcccacg taggtgattt tggcgactgc
     3121 aagattccac tatcaaaccc catgattgcc aatggaagtt taaaagacga gcacggagac
     3181 acacgacggg cacattctcc ggtctcatat aaggtatttc cggtaatggc gtctgcgatg
     3241 atggtgcatt taagcgtttc aacctcatca gaagccacgg aactgattgg gagagtggcg
     3301 gaaacgaaca ccaacatatg cagcagcaaa atttttttca tcaaggtatc cttcattgct
     3361 ccgctgtgcg cttaataggt gcataacttt gttttagaca ttatttgccg actaccttgg
     3421 tgatctcgcc tttcacgtag tgaacaaatt cttccaactg atctgcgcgc gaggccaagc
     3481 gatcttcttg tccaagataa gcctgcctag cttcaagtat gacgggctga tactgggccg
     3541 gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg
     3601 cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg
     3661 gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa
     3721 ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt
     3781 ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa
     3841 tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa
     3901 tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag
     3961 gggaagccga agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc
     4021 ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg
     4081 cggagccgta caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa
     4141 ctacctctga tagttgagtc gatacttcgg cgatcaccgc ttccctcatg atgtttaacg
     4201 tttgacatga ggggcggcca agggcgccag cccttggacg tccccctcga tggaagggtt
     4261 aggcaacact gcgtgttcgc tcgaatgcct ggcgtgtttg aaccatgtac acggctggac
     4321 catctggggt ggttacggta ccttgcctct caaaccccgc tttctcgtag catcggatcg
     4381 ctcgcaagtt gctcggcgac gggtccgttt ggatcttggt gacctcggga tcattgaaca
     4441 gcaactcaac cagagctcga accagcttgg ttcccaagcc tttgcccagt tgtgatgcat
     4501 tcgccagtna ctggtctatt ccgcgtactc ctggatcggt ttcttcttcc caccatccgt
     4561 ccccgcttcc aagagcaacg tacgactggg catacccaat cggctctcca ttcagcattg
     4621 caatgtatgg agtgacggac tcttgcgcta aaacgcttgg caagtactgt tcctgtacgt
     4681 cagcaagtgt cgggcgtgct tcttctccgc cccaccactc gacgatatga gatcgattta
     4741 gccactcata gagcatcgca aggtcatgct cagtcatgag gcgcagtgtg acggaatcgt
     4801 tgctgttggt cacgatgctg tactttgtga tgcctaactt tgtttggaaa tgttgaatac
     4861 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4921 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4981 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     5041 ggcgtatcac gaggcccttt cgtcttcaag aattttataa accgtggagc gggcaatact
     5101 gagctgatga gcaatttccg ttgcaccagt gcccttctga tgaagcgtca gcacgacgtt
     5161 cctgtccacg gtacgcctgc ggccaaattt gattcctttc agctttgctt cctgtcggcc
     5221 ctcattcgtg cgctctagga tcctccggcg ttcagcctgt gccacagccg acaggatggt
     5281 gaccaccatt tgccccatat caccgtcggt actgatcccg tcgtcaataa accgaaccgc
     5341 tacaccctga gcatcaaact cttttatcag ttggatcatg tcggcggtgt cgcggccaag
     5401 acggtcgagc ttcttcacca gaatgacatc accttcctcc accttcatcc tcagcaaatc
     5461 cagcccttcc cgatctgttg aactgccgga tgccttgtcg gtaaagatgc ggttagcttt
     5521 tacccctgca tctttgagcg ctctgatctg aatatcgagg gactgctggc tggttgagac
     5581 ccgcgcataa ccaaaaattc gcataaaaat gtaccttaaa tcgaatatca gacacgatgt
     5641 gtctattatg ccaaaatgac gatttaatgg acactcaaac gaagccgttt tactatgtct
     5701 gataatttat aacatttcgt acggttggaa aaatgttact aaatgcccgt caggcaggga
     5761 ggccgatatg cccgttgact ttctgaccac tgagcagact gaaagctatg gcagattcac
     5821 cggtgaaccg gatgagcttc agctggcacg atattttcac cttgatgaag cagacaagga
     5881 atttatcgga aaaagcagag gtgatcacaa ccgtctgggc attgccctgc aaattggatg
     5941 tgtccgtttt ctgggcacct tcctcaccga tatgaatcat attccttccg gcgtccggca
     6001 ttttaccgcc agacagctcg ggattcgtga tatcaccgtt cttgcagaat acggtcagag
     6061 ggaaaatacc cgccgtgagc atgcagcgct gatacgtcag cactatcagt atcgtgaatt
     6121 tgcctggccc tggacatttc gccttacccg tcttttatat acccggagct ggataagcaa
     6181 cgaacgtcct ggcctgcttt tcgatctggc gacagggtgg cttatgcaac atcgtattat
     6241 tctccccgga gccactacgc tgacccggtt gatttcagag gtaagggaaa aggcgacgtt
     6301 gcgcctgtgg aacaaactgg cactgatacc gtcagccgaa cagcgttcac agctggagat
     6361 gctgctgggg ccaactgatt gcagccgcct gtctttactg gaatcactga aaaagggccc
     6421 tgtgaccatc agtggtccgg cgtttaatga agcaattgaa cgctggaaaa ctctgaacga
     6481 ttttggcctg catgctgaaa acctgagtac actcccggct gtgcgcctga aaaatctcgc
     6541 acgttatgct ggtatgactt cggtgttcaa tattgccagg atgtcaccgc agaaaaggat
     6601 ggcggttctg gttgcctttg tccttgcatg ggaaacgctg gcgctggatg atgcattgga
     6661 cgttctggac gccatgctgg ccgttatcat ccgtgacgcc agaaagattg ggcagaaaaa
     6721 acggctccgc tcgctgaagg atctggataa atctgcattg gcgctcgcca gcgcatgttc
     6781 gtacctgctg aaagaagaaa caccggacga atcgattcgt gctgaggtgt tcagctacat
     6841 cccaaggcaa aagctggctg aaatcatcac gcttgtccgt gaaattgccc ggccctcaga
     6901 cgataatttt catgaagaaa tggtggagca gtacgggcgc gttcgtcgtt tcctgcccca
     6961 tctgctgaat accgttaaat tttcatccgc acctgccggg gttaccactc tgaatgcctg
     7021 tgactacctc agccgggagt tcagctcacg gcggcagttt tttgacgacg caccaacgga
     7081 aattatcagt cggtcatgga aacggctggt gattaacaag gaaaaacata tcacccgcag
     7141 gggatacacg ctctgctttc tcagtaaact gcaggatagt ctgaggcgga gggatgtcta
     7201 cgttaccggc agtaaccggt ggggagatcc tcgtgcaaga ttactacagg gtgctgactg
     7261 gcaggcaaac cggattaagg tttatcgttc tttggggcac ccgacagacc cgcaggaagc
     7321 aataaaatct ctgggccatc agcttgatag tcgttacaga caggttgctg cacgtcttgg
     7381 cgaaaatgag gctgtcgaac tcgatgtttc tggcccgaag ccccggttga caatttctcc
     7441 cctcgccagt cttgatgagc cggacagtct gaaacgactg agcaaaatga tcagtgatct
     7501 gctccctccg gtggatttaa cggagttgct gctcgaaatt aacgcccata ccggatttgc
     7561 tgatgagttt ttccatgcca gtgaagccag tgccagagtt gatgatctgc ccgtcagcat
     7621 cagcgccgtg ctgatggctg aagcctgcaa tatcggtctg gaaccactga tcagatcaaa
     7681 tgttcctgca ctgacccgac accggctgaa ctggacaaaa gcgaactatc tgcgggctga
     7741 aactatcacc agcgctaatg ccagactggt tgattttcag gcaacgctgc cactggcaca
     7801 gatatggggt ggaggagaag tggcatctgc agatggaatg cgctttgtta cgccagtcag
     7861 aacaatcaat gccggaccga accgcaaata ctttggtaat aacagaggga tcacctggta
     7921 caactttgtg tccgatcagt attccggctt tcatggcatc gttataccgg ggacgctgag
     7981 ggactctatc tttgtgctgg aaggccttct ggaacaggag accgggctga atccaaccga
     8041 aattatgacc gatacggcag gtgccagcga tcttgtcttt ggccttttct ggctgctggg
     8101 ataccagttt tctccacgcc tggctgatgc cggtgcttcg gttttctggc gaatggacca
     8161 tgatgccgac tatggcgtgc tgaatgatat tgccagaggg caatcagatc cccgaaaaat
     8221 agtccttcag tgggacgaaa tgatccggac cgcaggctcc ctgaagctgg gcaaagtaca
     8281 ggcctcagtg ctggtccgtt cattgctgaa aagtgaacgt ccctccggac tgactcaggc
     8341 aatcattgaa gtggggcgca tcaacaaaac gctgtatctg cttaattata ttgatgatga
     8401 agattaccgc cggcgcattc tgacccagct taatcgggga gaaagccgtc atgcagttgc
     8461 cagagccatc tgtcacggtc aaaaaggtga gataagaaaa cgatataccg acggtcagga
     8521 agatcagttg ggagctctgg ggctggtcac taacgccgtc gtgttatgga acactattta
     8581 tatgcaggca gctctggatc atctccgggc gcagggtgaa acactgaatg atgaagatat
     8641 cgcacgcctc tccccgcttt gccacggaca tatcaatatg ctcggccatt attccttcac
     8701 gctggcagaa ctggtgacca aaggacatct gagaccatta aaagaggcgt cagaggcaga
     8761 aaacgttgct taacgtgagt tttcgttcca ctgagcgtca gaccccagat ccaagtcgtg
     8821 gacgactcta tggcgaatcg ctgacgaatt gcccacgatt catatgtgaa tcacgcttta
     8881 ttatcatatt gttatgatga atcgactctt gattcaattc atcactttgg gtgttttttg
     8941 ctcatggtga ctcttgattc aattcacgat tcgaatcgcc ggtgaatcgt gtggtgagtc
     9001 gtccggcgac ttgccataac gtgattgccg gccgtcgacg aacccgattc ctgaaggtgc
     9061 tgaaaaccgg ttttccggct gcgccggcaa cgcttcacgg ccacgcaata aattgcgtcc
     9121 ccgttcaccg ttttcggagg agaagaacgc cggaaacagg acttcccctg gctgaatggt
     9181 gtgaatttcc gcgctatgca cttgcgcgca tactcatgca tgccgtaaaa acagagcctg
     9241 cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt taccggtttc ccgtcagaaa
     9301 cgccctgagg gcctctcagg cggtgctgat aacggatgtt atggtaaatg ttggatagca
     9361 aggactatca gtctggcaat gctacatatc attgctaatg gatttacgag caaaatgccg
     9421 aatgttgtcg cagataatcc gcgaacaatg atattggcaa gaagtccgcc aacaatgagg
     9481 gataaaaagg tataaccagc aactttcagt aacgacagat ctccccatat cacctctact
     9541 atcggataga taaaaacacc aacgccagca ggaagcgtag taaccatcaa cagtaaatgg
     9601 cttttagtgg gaccgtactc tctgacattt ccaactttca tcatgtgtgt gatgaatgac
     9661 gatatccacc ataggatcat gatcataagg aaattcataa ttatttactc tcgatgcgta
     9721 gatcaggtgc tttctgtaga tttggtcgta catactcgct gtctctttcg tcaagacaac
     9781 ggaagtagag tgttactgtg gtatctatcc ccattcttgt gttagccacg ttttcgttag
     9841 tgagcaggaa ttttttaccg aggctattac atttctcaga acctgtttta aaggcacttt
     9901 gacgtgctga tgccgcatct ctaaaaccat cagcagttgc tgaaaccgta tatgtatccc
     9961 tacctaactc aagaacgttg ctggtagaaa cacaaccaac tgtaagcagc gatattgata
    10021 aaatacctaa cctataaatc attgatttta ttacccaagt tgggtgattg tggatcatga
    10081 ttatatctgt tttttttatt attaaaatca aggggttcaa taattttctt caaagaactg
    10141 gtgtcgtgga gtggtgctgg tttccctttg gggggggagg gatggaatag gatcctcttg
    10201 agatcctttc tgttgcgtcg taatctcttg ccctggaaac gaaaaaaccg cccagcaagg
    10261 cggttttttc gaaggttctt agagcagcaa ctctttgaac cgaggtaact ggctttcgca
    10321 gagcacagaa accaaattag ttattacagt gtagctgtac ttcacctcac acttcaacac
    10381 ttctttgttc tgcgaatccg ttaccagtgg ctgctgccag tggcgataag tcgtgtcttt
    10441 ccggattgga ctcaagacga tagttaccgg ataaggcgca gcagtcggac tgaacggggg
    10501 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
    10561 gaatgagata aacgcggcca taacagcgga gtgacaccgg taaaccgaaa ggcaggaaca
    10621 ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg
    10681 tttcgccacc actgatttga gcgtccgttt ctgcgatgct tgtcaggggg gcggagccta
    10741 tggaaaaacg gcaacgccgt ggcctttcct ttttttcctc ctttgaccgc tcggttttct
    10801 cgagcttttc tatgcatgct cctgccgctc gccgcagccg aacgaccgga gcgtagcgac
    10861 tgagtgagcg aggaagcgga aaagagacag gttttacact gagcactgac gctctgaggc
    10921 ccttt
//
