LOCUS       CP006625                4072 bp    DNA     circular BCT 26-JUL-2016
DEFINITION  Enterococcus faecium Aus0085 plasmid p5, complete sequence.
ACCESSION   CP006625
VERSION     CP006625.1
DBLINK      BioProject: PRJNA193299
            BioSample: SAMN02604219
KEYWORDS    .
SOURCE      Enterococcus faecium Aus0085
  ORGANISM  Enterococcus faecium Aus0085
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Lam,M.M., Seemann,T., Tobias,N.J., Chen,H., Haring,V., Moore,R.J.,
            Ballard,S., Grayson,L.M., Johnson,P.D., Howden,B.P. and Stinear,T.P.
  TITLE     Comparative analysis of the complete genome of an epidemic hospital
            sequence type 203 clone of vancomycin-resistant Enterococcus faecium
  JOURNAL   BMC Genomics 14, 595 (2013)
   PUBMED   24004955
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Lam,M.M.C., Seemann,T., Tobias,N.J., Chen,H., Haring,V., Moore,R.J.,
            Ballard,S., Grayson,L., Johnson,P.D.R., Howden,B.P. and Stinear,T.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-2013) Microbiology and Immunology, University of
            Melbourne, Royal Parade, Parkville, Victoria 3010, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Newbler v. 2.8
            Genome Coverage       :: 40X
            Sequencing Technology :: 454
            ##Genome-Assembly-Data-END##
            Source Bacteria and DNA are available from Professor Benjamin
            Howden, Austin Hospital, Melbourne, Australia
            (benjamin.Howden@austin.org.au).
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Enterococcus faecium Aus0085"
                     /mol_type="genomic DNA"
                     /strain="AUS0085"
                     /db_xref="taxon:1305849"
                     /plasmid="p5"
     gene            1..897
                     /locus_tag="EFAU085_p5001"
     CDS             1..897
                     /locus_tag="EFAU085_p5001"
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation factor"
                     /protein_id="AGS77173.1"
                     /translation="MDTPLKIQWSIDRITIVGNLKENIYYHTTHDVLILDFEQLMRLNE
                     GNGYLRSVSNNGWQLLDQHEENIAYIEILKWQEGKGRIDFNPNKISQFLASSMKNFIHD
                     LFIEPHFSRADIACDIIDVPDEFITQYRVVDPVSFKPIYGRNGKLETAYWGSRSSERQI
                     RMYNKKLEQEKKRKIVPKEIVSWWRLELQLRRGKATDWHAMVYESLDSFASPHYLPADT
                     SVADKMMITALTTEHDYWGQINRKTKYKYRNLLKQESQNDELTNHLRETFAESADDLKK
                     ELDTWLLGLDVTEEEEK"
                     /name="Replication initiation factor"
     gene            855..1127
                     /locus_tag="EFAU085_p5002"
     CDS             855..1127
                     /locus_tag="EFAU085_p5002"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AGS77174.1"
                     /translation="MASRARRNRGGRKMTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKM
                     SGKLLDVTQYELILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
                     /name="hypothetical protein"
     gene            1120..1545
                     /locus_tag="EFAU085_p5003"
     CDS             1120..1545
                     /locus_tag="EFAU085_p5003"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AGS77175.1"
                     /translation="MTDLSEKMTIKNLADELGVSKIKESEMVAVLEMRCNMDKIDLREE
                     TVDRIELMYERLADYLNLPDEDNYSEMLERMMDVLNSTINVGLANSSLITDKFVYGKIA
                     ETEDESEKEFWLNILTYVMQLNQIGVIYRNKSKGKEL"
                     /name="hypothetical protein"
     gene            1555..1863
                     /locus_tag="EFAU085_p5004"
     CDS             1555..1863
                     /locus_tag="EFAU085_p5004"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AGS77176.1"
                     /translation="MTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKHFGF
                     ESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNESDGLLGVIPSKKVR"
                     /name="hypothetical protein"
     gene            2131..3546
                     /gene="mob"
                     /locus_tag="EFAU085_p5005"
     CDS             2131..3546
                     /gene="mob"
                     /locus_tag="EFAU085_p5005"
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme Mob"
                     /protein_id="AGS77177.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESISAEMRYISKQREKNEREK
                     ALVASEKAKIEEEKKSIIDLEKELAVVFQKDPVPLNSFEKLSDGSYRLSSADFVDLYQK
                     ASNATNSKKYADMANESLGNTLDKNIALFDKNMALEQENSFLKNQQKVDVSAVVAENKK
                     LKEENRSLRRRLTQLKNAFQKSITRLSIRFGLVEKDMGISEELKILDQEKTLASQQNAP
                     ERNIGRSENEMEW"
                     /name="mob"
     gene            3556..3615
                     /locus_tag="EFAU085_p5006"
     ncRNA           3556..3615
                     /ncRNA_class="scRNA"
                     /locus_tag="EFAU085_p5006"
     Typing          2..897
                     /name="rep_cluster_2146"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2099..3543
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.34"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 atggacaccc ccctaaaaat tcagtggtca atcgaccgaa ttaccattgt tggaaatctt
       61 aaagagaata tctattatca cactacacat gatgtgttga ttttagattt tgaacagctt
      121 atgcgcctaa atgaaggtaa cggttattta agatctgtta gtaataatgg ttggcagttg
      181 ttagaccaac atgaagagaa tattgcttat attgaaattt taaaatggca agaggggaaa
      241 gggcgtatag acttcaatcc gaataagatt agtcaattct tagctagttc catgaaaaat
      301 tttattcatg atttgtttat cgaacctcac ttttcacgtg cagatattgc ttgcgacatt
      361 atcgatgtac ctgatgagtt tattacacaa tatcgggtag ttgatccagt ttcttttaag
      421 cctatttatg gacggaatgg aaaattagag acggcatatt ggggatctcg ctcttctgaa
      481 cgtcaaattc ggatgtataa taaaaaactg gaacaagaga agaaaagaaa aattgtgcca
      541 aaagaaatcg tgagttggtg gcggttagaa ttacaattaa gacgagggaa agcgactgat
      601 tggcatgcta tggtttatga aagcttagat agttttgcta gtcctcatta cttgcctgct
      661 gatacaagtg tagctgacaa aatgatgatt actgctttaa caacagaaca tgattattgg
      721 gggcaaatta atcgaaagac aaaatataaa tatcgaaatt tgctgaagca agaaagtcaa
      781 aatgatgaat tgacgaatca tttacgagaa acctttgcag aatcggcaga cgatttaaaa
      841 aaagaattag acacatggct tctagggcta gacgtaacag aggaggaaga aaaatgacag
      901 aaaaacaaaa gtattttggt aagaaaaatt ggttggtaaa tttaacaggt gattacgttg
      961 tggttgtttt tttggatagc aaaaaaatgt ccggtaagtt acttgatgta acgcaatatg
     1021 aattgatttt gaaaaataat aaaattgact ctccgattat tatccctaag cacgccgtta
     1081 aatatattta tgcggtagct gaaagtagtg gagaagctaa tgactgattt gtcagaaaag
     1141 atgaccataa aaaatcttgc tgatgaatta ggtgtatcga aaattaaaga atcggaaatg
     1201 gtggcagttt tggaaatgag gtgtaacatg gataaaattg atttaagaga agagacagtt
     1261 gatagaattg aattgatgta tgaacgatta gctgattatt tgaatttgcc ggatgaagat
     1321 aattatagtg aaatgcttga gagaatgatg gacgttctca attctacaat caatgtaggc
     1381 ctagcaaatt cttctttgat tactgataaa tttgtttacg gtaaaatagc cgaaacagaa
     1441 gatgaatctg aaaaggaatt ttggctgaat attttaactt atgttatgca attaaatcaa
     1501 attggtgtta tttatcgaaa taaatcaaaa ggcaaggagc tgtaaatctt gggaatgact
     1561 ttagcagata ttaaagcaca agaagaaaaa ttgaaaaaag aacaggcaaa acttcgtcaa
     1621 cagaaaaaac gattgattga gaaggaaaat gctgaaatgg gtaaggcact tcggaagcat
     1681 tttggatttg aatcttttga tgattttgag cggtggttat cgtcaatttc tgacgatcag
     1741 caacacgaaa aatcagtgga taatgagtgg cttgctgatg aaaaatcagc gccaagcgaa
     1801 cgagattatc ccaatgaatc tgacggctta ctcggagtga taccgagtaa aaaagtcaga
     1861 tgagtgagag taaatttacg tcaatgttta aatgacgtga gtttacgata agcgattatt
     1921 ttgcgtgaag caaaataaag cgtaaaagga gttgagagcg cgacacgctt tcggctcctt
     1981 ttttcgtgtc acttgtgaca gcgaataccc tcggagagca tttggaattg tgacagccat
     2041 gtcacaatta ggaaaatgtt actctttgac aaagagtaac agaaaggttt acactctttt
     2101 attagcgaca gcgcgaagga gggttttaac ttggggttta gtatttcatt taaaaaagga
     2161 acgaagaata caagtatttc tcataataat cgtgagttaa ctgatagcca gaaaaccaat
     2221 gattggcata agcatattga cttcacgaaa tcggatgaaa atatttattt agagcagacg
     2281 aatctagcag ataaatatga ggaattgttt ggcgaagtgg tagaaaaata taatgcgaaa
     2341 caaaaaagag ccgatagaaa aatcgaggac tatttagcga aagtgcgcaa ggataaaaag
     2401 ttggaacctc aacgagaatt tattgtgcaa ataggcattt tagatgattt ccagacaaat
     2461 cgagcagatg gttcttctac tggcatatcg aaacaacaag ctgaacaaaa tcgagtgatt
     2521 gccaataaaa ttttggttca gtattacaag gagtttcaag agagaaaccc taatttggct
     2581 gtttataatg ctgttattca taatgacgaa ataagtccgc acctgcactt gaatattgtg
     2641 ccggttgctg aaggttataa acggggtgtt caaaagcagc cgagctttaa taaagcactg
     2701 gttcaacaag ggatacacgc tgataaagat aatggtcgag tgcttttcaa taattttaga
     2761 aatcaagaag ttgactctat agaacgatta atgaatgatt acggttggaa aagggatatt
     2821 gtcggtacca acaaaattaa agatattcac gaatacaaag aaatcatgag tgaaatttct
     2881 gaattgcgcc aaacggctgt aaaagagcgt gagagcattt ctgcggaaat gaggtatatt
     2941 tctaagcaaa gagaaaaaaa cgagcgtgag aaggctctgg tggcttctga gaaggcgaaa
     3001 attgaagaag agaaaaaatc gattatcgat ttggaaaaag agctggctgt tgtatttcaa
     3061 aaagatcctg tgccactcaa tagttttgaa aaattatcag atggttctta tcgcttatct
     3121 agtgctgatt ttgtagattt gtatcaaaaa gcgtctaatg ctacgaacag caaaaaatat
     3181 gctgatatgg cgaatgaatc gcttggaaat acattagata aaaatatcgc tttatttgat
     3241 aaaaacatgg cgttagaaca agaaaattct tttttgaaaa atcaacaaaa ggttgatgtg
     3301 tcagcggttg ttgctgaaaa caaaaagctg aaggaagaaa atcgttcttt gcgtcggcgg
     3361 ttaactcaac tgaaaaacgc atttcaaaaa tcaatcactc gtttatcaat tcgttttggg
     3421 cttgtagaaa aagatatggg aatatctgaa gagctaaaaa tattagatca agagaaaacg
     3481 ttggctagtc agcagaatgc cccagaacgt aatatagggc gttctgagaa tgagatggag
     3541 tggtagttat acggtgcagt caacggtacc ggaggcggtc tgtgccgagg ataaggagtt
     3601 gacggctaaa ccactaatcc ctgaagctga catttgctaa aaaatggcaa gtccattttt
     3661 tggccaactt agcgttagcg aagtttgggg gcgccctttt caaagggtgc ggttttatgt
     3721 ttttggatat ttgcatagct aaagagaagt gctattattg agttatcaaa taaataaact
     3781 gaaaaaagag caaaaaaata aagtcgagca acgctggaca cgttctcgac ctttacggaa
     3841 actaatcaca ctagctccgt aactcaaatt cattatacga ataatttcag gtttcttcaa
     3901 gactagagat tgctggttat gaatttttta gagttacgtg aactctggaa aattctttcc
     3961 accaatctct tttttctttg aggaggaact aaatttgagt aaaaaaggca cgatcataaa
     4021 tgagtgggaa aaattaacag ccccctctaa caggcggtta agtgtccctt tt
//
