LOCUS       CP004059               11067 bp    DNA     circular BCT 03-SEP-2015
DEFINITION  Salmonella enterica subsp. enterica serovar Typhimurium str. U288
            plasmid pSTU288-2, complete sequence.
ACCESSION   CP004059
VERSION     CP004059.1
DBLINK      BioProject: PRJNA157919
            BioSample: SAMN02604233
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Typhimurium str. U288
  ORGANISM  Salmonella enterica subsp. enterica serovar Typhimurium str. U288
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 11067)
  AUTHORS   Hooton,S.P., Timms,A.R., Cummings,N.J., Moreton,J., Wilson,R. and
            Connerton,I.F.
  TITLE     The complete plasmid sequences of Salmonella enterica serovar
            Typhimurium U288
  JOURNAL   Plasmid 76, 32-39 (2014)
   PUBMED   25175817
REFERENCE   2  (bases 1 to 11067)
  AUTHORS   Hooton,S.P.T., Timms,A.R., Roswell,J., Wilson,R. and Connerton,I.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2013) School of Biosciences, Division of Food
            Sciences, University of Nottingham, Sutton Bonington Campus, Sutton
            Bonington Near Loughborough, Leicestershire LE12 5RD, United Kingdom
COMMENT     Annotation was added by the NCBI Prokaryotic Genomes Automatic
            Annotation Pipeline Group. Information about the Pipeline can be
            found here:
            http://www.ncbi.nlm.nih.gov/genomes/static/Pipeline.html. Please be
            aware that the annotation is done automatically with little or no
            manual curation.
            The source DNA is available from our laboratory (University of
            Nottingham, School of Biosciences, Division of Food Sciences, Food
            Microbiology Department, Sutton Bonington Campus, Leicestershire
            LE12 5RD) and enquiries should be made to Prof. Ian Connerton at
            ian.connerton@nottingham.ac.uk.
FEATURES             Location/Qualifiers
     source          1..11067
                     /organism="Salmonella enterica subsp. enterica serovar
                     Typhimurium str. U288"
                     /mol_type="genomic DNA"
                     /strain="U288"
                     /serovar="Typhimurium"
                     /sub_species="enterica"
                     /db_xref="taxon:1171376"
                     /plasmid="pSTU288-2"
     gene            complement(513..797)
                     /locus_tag="STU288_2p00005"
     CDS             complement(513..797)
                     /locus_tag="STU288_2p00005"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C, MobC"
                     /protein_id="AGK12400.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C, MobC"
     gene            996..3125
                     /locus_tag="STU288_2p00010"
     CDS             996..3125
                     /locus_tag="STU288_2p00010"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein A, MobA"
                     /protein_id="AGK12401.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAER
                     LPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPK
                     AWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRT
                     DRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGR
                     RSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGV
                     ALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQ
                     LKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHG
                     LVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEY
                     DADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQI
                     EQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQK
                     LASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPA
                     PRQRGMDRGGPDFSM"
                     /name="mobilization protein A, MobA"
     gene            3186..3398
                     /locus_tag="STU288_2p00015"
     CDS             3186..3398
                     /locus_tag="STU288_2p00015"
                     /codon_start=1
                     /transl_table=11
                     /product="unknown protein E"
                     /protein_id="AGK12402.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="unknown protein E"
     gene            3400..3606
                     /locus_tag="STU288_2p00020"
     CDS             3400..3606
                     /locus_tag="STU288_2p00020"
                     /codon_start=1
                     /transl_table=11
                     /product="repressor protein F"
                     /protein_id="AGK12403.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="repressor protein F"
     gene            3636..4475
                     /locus_tag="STU288_2p00025"
     CDS             3636..4475
                     /locus_tag="STU288_2p00025"
                     /note="COG3598 RecA-family ATPase"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein A, RepA"
                     /protein_id="AGK12404.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="replication protein A, RepA"
     gene            4462..5313
                     /locus_tag="STU288_2p00030"
     CDS             4462..5313
                     /locus_tag="STU288_2p00030"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C"
                     /protein_id="AGK12405.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="replication protein C"
     gene            5621..6436
                     /locus_tag="STU288_2p00035"
     CDS             5621..6436
                     /locus_tag="STU288_2p00035"
                     /note="COG0294 Dihydropteroate synthase and related
                     enzymes"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="AGK12406.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADVI
                     DLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAYLN
                     DIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAALTG
                     AGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRALTGR
                     GPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
                     /name="dihydropteroate synthase"
     gene            6497..7300
                     /locus_tag="STU288_2p00040"
     CDS             6497..7300
                     /locus_tag="STU288_2p00040"
                     /note="COG3231 Aminoglycoside phosphotransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="StrA"
                     /protein_id="AGK12407.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRR
                     GELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPS
                     MGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARV
                     ERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIA
                     NAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
                     /name="StrA"
     gene            7300..8136
                     /locus_tag="STU288_2p00045"
     CDS             7300..8136
                     /locus_tag="STU288_2p00045"
                     /note="COG3570 Streptomycin 6-kinase"
                     /codon_start=1
                     /transl_table=11
                     /product="streptomycin 3''-kinase"
                     /protein_id="AGK12408.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="streptomycin 3''-kinase"
     gene            8523..8795
                     /locus_tag="STU288_2p00050"
     CDS             8523..8795
                     /locus_tag="STU288_2p00050"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AGK12409.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGEGMLSTWLCCSEWVALRQRS"
                     /name="hypothetical protein"
     misc_feature    complement(8796..9472)
                     /note="potential frameshift: common BLAST hit:
                     gi|431805260|ref|YP_007232162.1| tetracycline repressor
                     protein"
     gene            9476..10750
                     /locus_tag="STU288_2p00065"
     CDS             9476..10750
                     /locus_tag="STU288_2p00065"
                     /note="COG0477 Permeases of the major facilitator
                     superfamily"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux protein"
                     /protein_id="AGK12410.1"
                     /translation="MSTNLSVIKNPRVQSDQRRLVRRPDVKPNRPLIVILSTVALDAVG
                     IGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVS
                     LAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADITDGDERARHFGFMSA
                     CFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREAL
                     NPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFG
                     ILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGI
                     GMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAG
                     AALYLLCLPALRRGLWSGAGQRADR"
                     /name="tetracycline efflux protein"
     gene            complement(10681..>11067)
                     /locus_tag="STU288_2p00070"
     CDS             complement(10681..>11067)
                     /locus_tag="STU288_2p00070"
                     /note="COG0454 Histone acetyltransferase HPA2 and related
                     acetyltransferases"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acetyltransferase"
                     /protein_id="AGK12411.1"
                     /translation="APEMFGSVYEHEEKKPMEAFAERLRDAVAFGAYIDGEIIGLSVFK
                     QEDGPKDAHKAHLSGVFVEPEQRGRGVAGMLLRAVIDYGREHVEQIMLTVAEGNEAAIN
                     LYQRSARCPAPLQSPRRSAGRQSK"
                     /name="putative acetyltransferase"
     ARG             5622..6433
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             9552..10747
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             7301..8133
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             6498..7297
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             6882..7300
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     Typing          752..907
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          3654..4475
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          997..3122
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttcttcagg gccgacaatc ggatctcgcc gaaatgcctg ccgttgctag acattgccag
       61 ccagtgcccg tcactcccgt actaactgtc acgaacccct gcaataactg tcacgccccc
      121 ctgcaataac tgtcacgaac ccctgcaata actgtcacgc ccccaaacct gcaaacccag
      181 caggggcggg ggctggcggg gtgttggaaa aatccatcca tgattatcta agaataatcc
      241 actaggcgcg gttatcagcg cccttgtggg gcgctgctgc ccttgcccaa tatgcccggc
      301 cagaggccgg atagctggtc tattcgctgc gctaggctac acaccgcccc accgctgcgc
      361 ggcaggggga aaggcgggca aagcccgcta aaccccacac caaaccccgc agaaatacgc
      421 tggagcgctt ttagccgctt tagcggcctt tccccctacc cgaagggtgg gggcgcgtgt
      481 gcagccccgc agggcctgtc tcggtcgatc attcagcccg gctcatcctt ctggcgtggc
      541 ggcagaccga acaaggcgcg gtcgtggtcg cgttcaaggt acgcatccat tgccgccatg
      601 agccgatcct ccggccactc gctgctgttc accttggcca aaatcatggc ccccaccagc
      661 accttgcgcc ttgtttcgtt cttgcgctct tgctgctgtt cccttgcccg cacccgctga
      721 atttcggcat tgattcgcgc tcgttgttct tcgagcttgg ccagccgatc cgccgccttg
      781 ttgctcccct taaccatctt gacaccccat tgttaatgtg ctgtctcgta ggctatcatg
      841 gaggcacagc ggcggcaatc ccgaccctac tttgtagggg agggcgcact taccggtttc
      901 tcttcgagaa actggcctaa cggccaccct tcgggcggtg cgctctccga gggccattgc
      961 atggagccga aaagcaaaag caacagcgag gcagcatggc gatttatcac cttacggcga
     1021 aaaccggcag caggtcgggc ggccaatcgg ccagggccaa ggccgactac atccagcgcg
     1081 aaggcaagta tgcccgcgac atggatgaag tcttgcacgc cgaatccggg cacatgccgg
     1141 agttcgtcga gcggcccgcc gactactggg atgctgccga cctgtatgaa cgcgccaatg
     1201 ggcggctgtt caaggaggtc gaatttgccc tgccggtcga gctgaccctc gaccagcaga
     1261 aggcgctggc gtccgagttc gcccagcacc tgactggtgc cgagcgcctg ccgtatacgc
     1321 tggccatcca tgccggtggc ggcgagaacc cgcactgcca cctgatgatc tccgagcgga
     1381 tcaatgacgg catcgagcgg cccgccgctc agtggttcaa gcggtacaac ggcaagaccc
     1441 cggagaaggg cggggcacag aagaccgaag cgctcaagcc caaggcatgg cttgagcaga
     1501 cccgcgaggc atgggccgac catgccaacc gggcattaga gcgggctggc cacgacgccc
     1561 gcattgacca cagaacactt gaggcgcagg gcatcgagcg cctgcccggt gttcacctgg
     1621 ggccgaacgt ggtggagatg gaaggccggg gcatccgcac cgaccgggca gacgtggccc
     1681 tgaacatcga caccgccaac gcccagatca tcgacttaca ggaataccgg gaggcaatag
     1741 accatgaacg caatcgacag agtgaagaaa tccagaggca tcaacgagtt agcggagcag
     1801 atcgaaccgc tggcccagag catggcgaca ctggccgacg aagcccggca ggtcatgagc
     1861 cagacccagc aggccagcga ggcgcaggcg gcggagtggc tgaaagccca gcgccagaca
     1921 ggggcggcat gggtggagct ggccaaagag ttgcgggagg tagccgccga ggtgagcagc
     1981 gccgcgcaga gcgcccggag cgcgtcgcgg gggtggcact ggaagctatg gctaaccgtg
     2041 atgctggctt ccatgatgcc tacggtggtg ctgctgatcg catcgttgct cttgctcgac
     2101 ctgacgccac tgacaaccga ggacggctcg atctggctgc gcttggtggc ccgatgaaga
     2161 acgacaggac tttgcaggcc ataggccgac agctcaaggc catgggctgt gagcgcttcg
     2221 atatcggcgt cagggacgcc accaccggcc agatgatgaa ccgggaatgg tcagccgccg
     2281 aagtgctcca gaacacgcca tggctcaagc ggatgaatgc ccagggcaat gacgtgtata
     2341 tcaggcccgc cgagcaggag cggcatggtc tggtgctggt ggacgacctc agcgagtttg
     2401 acctggatga catgaaagcc gagggccggg agcctgccct ggtagtggaa accagcccga
     2461 agaactatca ggcatgggtc aaggtggccg acgccgcagg cggtgaactt cgggggcaga
     2521 ttgcccggac gctggccagc gagtacgacg ccgacccggc cagcgccgac agccgccact
     2581 atggccgctt ggcgggcttc accaaccgca aggacaagca caccacccgc gccggttatc
     2641 agccgtgggt gctgctgcgt gaatccaagg gcaagaccgc caccgctggc ccggcgctgg
     2701 tgcagcaggc tggccagcag atcgagcagg cccagcggca gcaggagaag gcccgcaggc
     2761 tggccagcct cgaactgccc gagcggcagc ttagccgcca ccggcgcacg gcgctggacg
     2821 agtaccgcag cgagatggcc gggctggtca agcgcttcgg tgatgacctc agcaagtgcg
     2881 actttatcgc cgcgcagaag ctggccagcc ggggccgcag tgccgaggaa atcggcaagg
     2941 ccatggccga ggccagccca gcgctggcag agcgcaagcc cggccacgaa gcggattaca
     3001 tcgagcgcac cgtcagcaag gtcatgggtc tgcccagcgt ccagcttgcg cgggccgagc
     3061 tggcacgggc accggcaccc cgccagcgag gcatggacag gggcgggcca gatttcagca
     3121 tgtagtgctt gcgttggtac tcacgcctgt tatactatga gtactcacgc acagaagggg
     3181 gttttatgga atacgaaaaa agcgcttcag ggtcggtcta cctgatcaaa agtgacaagg
     3241 gctattggtt gcccggtggc tttggttata cgtcaaacaa ggccgaggct ggccgctttt
     3301 cagtcgctga tatggccagc cttaaccttg acggctgcac cttgtccttg ttccgcgaag
     3361 acaagccttt cggccccggc aagtttctcg gtgactgata tgaaagacca aaaggacaag
     3421 cagaccggcg acctgctggc cagccctgac gctgtacgcc aagcgcgata tgccgagcgc
     3481 atgaaggcca aagggatgcg tcagcgcaag ttctggctga ccgacgacga atacgaggcg
     3541 ctgcgcgagt gcctggaaga actcagagcg gcgcagggcg ggggtagtga ccccgccagc
     3601 gcctaaccac caactgcctg caaaggaggc aatcaatggc tacccataag cctatcaata
     3661 ttctggaggc gttcgcagca gcgccgccac cgctggacta cgttttgccc aacatggtgg
     3721 ccggtacggt cggggcgctg gtgtcgcccg gtggtgccgg taaatccatg ctggccctgc
     3781 aactggccgc acagattgca ggcgggccgg atctgctgga ggtgggcgaa ctgcccaccg
     3841 gcccggtgat ctacctgccc gccgaagacc cgcccaccgc cattcatcac cgcctgcacg
     3901 cccttggggc gcacctcagc gccgaggaac ggcaagccgt ggctgacggc ctgctgatcc
     3961 agccgctgat cggcagcctg cccaacatca tggccccgga gtggttcgac ggcctcaagc
     4021 gcgccgccga gggccgccgc ctgatggtgc tggacacgct gcgccggttc cacatcgagg
     4081 aagaaaacgc cagcggcccc atggcccagg tcatcggtcg catggaggcc atcgccgccg
     4141 ataccgggtg ctctatcgtg ttcctgcacc atgccagcaa gggcgcggcc atgatgggcg
     4201 caggcgacca gcagcaggcc agccggggca gctcggtact ggtcgataac atccgctggc
     4261 agtcctacct gtcgagcatg accagcgccg aggccgagga atggggtgtg gacgacgacc
     4321 agcgccggtt cttcgtccgc ttcggtgtga gcaaggccaa ctatggcgca ccgttcgctg
     4381 atcggtggtt caggcggcat gacggcgggg tgctcaagcc cgccgtgctg gagaggcagc
     4441 gcaagagcaa gggggtgccc cgtggtgaag cctaagaaca agcacagcct cagccacgtc
     4501 cggcacgacc cggcgcactg tctggccccc ggcctgttcc gtgccctcaa gcggggcgag
     4561 cgcaagcgca gcaagctgga cgtgacgtat gactacggcg acggcaagcg gatcgagttc
     4621 agcggcccgg agccgctggg cgctgatgat ctgcgcatcc tgcaagggct ggtggccatg
     4681 gctgggccta atggcctagt gcttggcccg gaacccaaga ccgaaggcgg acggcagctc
     4741 cggctgttcc tggaacccaa gtgggaggcc gtcaccgctg atgccatggt ggtcaaaggt
     4801 agctatcggg cgctggcaaa ggaaatcggg gcagaggtcg atagtggtgg ggcgctcaag
     4861 cacatacagg actgcatcga gcgcctttgg aaggtatcca tcatcgccca gaatggccgc
     4921 aagcggcagg ggtttcggct gctgtcggag tacgccagcg acgaggcgga cgggcgcctg
     4981 tacgtggccc tgaacccctt gatcgcgcag gccgtcatgg gtggcggcca gcatgtgcgc
     5041 atcagcatgg acgaggtgcg ggcgctggac agcgaaaccg cccgcctgct gcaccagcgg
     5101 ctgtgtggct ggatcgaccc cggcaaaacc ggcaaggctt ccatagatac cttgtgcggc
     5161 tatgtctggc cgtcagaggc cagtggttcg accatgcgca agcgccgcca gcgggtgcgc
     5221 gaggcgttgc cggagctggt cgcgctgggc tggacggtaa ccgagttcgc ggcgggcaag
     5281 tacgacatca cccggcccaa ggcggcaggc tgaccccccc cactctattg taaacaagac
     5341 atttttatct tttatattca atggcttatt ttcctgctaa ttggtaatac catgaaaaat
     5401 accatgctca gaaaaggctt aacaatattt tgaaaaattg cctactgagc gctgccgcac
     5461 agctccatag gccgctttcc tggctttgct tccagatgta tgctcttctg ctcctgcagc
     5521 taatggatca ccgcaaacag gttactcgcc tggggattcc ctttcgaccc gagcatccgt
     5581 atgatactca tgctcgatta ttattattat agaagccccc atgaataaat cgctcatcat
     5641 tttcggcatc gtcaacataa cctcggacag tttctccgat ggaggccggt atctggcgcc
     5701 agacgcagcc attgcgcagg cgcgtaagct gatggccgag ggggcagatg tgatcgacct
     5761 cggtccggca tccagcaatc ccgacgccgc gcctgtttcg tccgacacag aaatcgcgcg
     5821 tatcgcgccg gtgctggacg cgctcaaggc agatggcatt cccgtctcgc tcgacagtta
     5881 tcaacccgcg acgcaagcct atgccttgtc gcgtggtgtg gcctatctca atgatattcg
     5941 cggttttcca gacgctgcgt tctatccgca attggcgaaa tcatctgcca aactcgtcgt
     6001 tatgcattcg gtgcaagacg ggcaggcaga tcggcgcgag gcacccgctg gcgacatcat
     6061 ggatcacatt gcggcgttct ttgacgcgcg catcgcggcg ctgacgggtg ccggtatcaa
     6121 acgcaaccgc cttgtccttg atcccggcat ggggtttttt ctgggggctg ctcccgaaac
     6181 ctcgctctcg gtgctggcgc ggttcgatga attgcggctg cgcttcgatt tgccggtgct
     6241 tctgtctgtt tcgcgcaaat cctttctgcg cgcgctcaca ggccgtggtc cgggggatgt
     6301 cggggccgcg acactcgctg cagagcttgc cgccgccgca ggtggagctg acttcatccg
     6361 cacacacgag ccgcgcccct tgcgcgacgg gctggcggta ttggcggcgc tgaaagaaac
     6421 cgcaagaatt cgttaactgc acattcggga tatttctcta tattcgcgct tcatcagaaa
     6481 actgaaggaa cctccattga atcgaactaa tatttttttt ggtgaatcgc attctgactg
     6541 gttgcctgtc agaggcggag aatctggtga ttttgttttt cgacgtggtg acgggcatgc
     6601 cttcgcgaaa atcgcacctg cttcccgccg cggtgagctc gctggagagc gtgaccgcct
     6661 catttggctc aaaggtcgag gtgtggcttg ccccgaggtc atcaactggc aggaggaaca
     6721 ggagggtgca tgcttggtga taacggcaat tccgggagta ccggcggctg atctgtctgg
     6781 agcggatttg ctcaaagcgt ggccgtcaat ggggcagcaa cttggcgctg ttcacagcct
     6841 atcggttgat caatgtccgt ttgagcgcag gctgtcgcga atgttcggac gcgccgttga
     6901 tgtggtgtcc cgcaatgccg tcaatcccga cttcttaccg gacgaggaca agagtacgcc
     6961 gcagctcgat cttttggctc gtgtcgaacg agagctaccg gtgcggctcg accaagagcg
     7021 caccgatatg gttgtttgcc atggtgatcc ctgcatgccg aacttcatgg tggaccctaa
     7081 aactcttcaa tgcacgggtc tgatcgacct tgggcggctc ggaacagcag atcgctatgc
     7141 cgatttggca ctcatgattg ctaacgccga agagaactgg gcagcgccag atgaagcaga
     7201 gcgcgccttc gctgtcctat tcaatgtatt ggggatcgaa gcccccgacc gcgaacgcct
     7261 tgccttctat ctgcgattgg accctctgac ttggggttga tgttcatgcc gcctgttttt
     7321 cctgctcatt ggcacgtttc gcaacctgtt ctcattgcgg acaccttttc cagcctcgtt
     7381 tggaaagttt cattgccaga cgggactcct gcaatcgtca agggattgaa acctatagaa
     7441 gacattgctg atgaactgcg cggggccgac tatctggtat ggcgcaatgg gaggggagca
     7501 gtccggttgc tcggtcgtga gaacaatctg atgttgctcg aatatgccgg ggagcgaatg
     7561 ctctctcaca tcgttgccga gcacggcgac taccaggcga ccgaaattgc agcggaacta
     7621 atggcgaagc tgtatgccgc atctgaggaa cccctgcctt ctgcccttct cccgatccgg
     7681 gatcgctttg cagctttgtt tcagcgggcg cgcgatgatc aaaacgcagg ttgtcaaact
     7741 gactacgtcc acgcggcgat tatagccgat caaatgatga gcaatgcctc ggaactgcgt
     7801 gggctacatg gcgatctgca tcatgaaaac atcatgttct ccagtcgcgg ctggctggtg
     7861 atagatcccg tcggtctggt cggtgaagtg ggctttggcg ccgccaatat gttctacgat
     7921 ccggctgaca gagacgacct ttgtctcgat cctagacgca ttgcacagat ggcggacgca
     7981 ttctctcgtg cgctggacgt cgatccgcgt cgcctgctcg accaggcgta cgcttatggg
     8041 tgcctttccg cagcttggaa cgcggatgga gaagaggagc aacgcgatct agctatcgcg
     8101 gccgcgatca agcaggtgcg acagacgtca tactagatat caagcgactt ctcctatccc
     8161 ctgggaacac atcaatctca ccggagaata tcgctggcca aagccttagc gtaggattcc
     8221 gccccttccc gcaaacgacc cctttctggc gcgtttgcag ggccatagac ggccgccagc
     8281 ccagcggcga gggcaaccag cccggtgagc gtcggaaagg cgctggaagc cccgtagcga
     8341 cgcggagagg ggcgagacaa gccaagggcg caggctcgat gcgcagcacg acatagccgg
     8401 ttctcgcaag gacgagaatt tccctgcggt gcccctcaag tgtcaatgaa agtttccaac
     8461 gcgagccatt cgcgagagcc ttgagtccac gctagatcta tctcatctgc gcaaggcaga
     8521 acgtgaagac ggccgccctg gacctcgccc gcgagcgcca ggcgcacgag gccggcgcgc
     8581 ggacccgcgc cacggcccac gagcggacgc cgcagcagga gcgccagaag gccgccagag
     8641 aggccgagcg cggccgtgag gcttggacgc tagggcaggg catgaaaaag cccgtagcgg
     8701 gctgctacgg gcgtctgacg cggtggaaag gggaggggat gttgtctaca tggctctgct
     8761 gtagtgagtg ggttgcgctc cggcagcggt cctgatcaat cgtcaccctt tctcggtcct
     8821 tcaacgttcc tgacaacgag cctccttttc gccaatccat cgacaatcac cgcgagtccc
     8881 tgctcgaacg ctgcgtccgg accggcttcg tcgaaggcgt ctatcgcggc ccgcaacagc
     8941 ggcgagagcg gagcctgttc aacggtgccg ccgcgctcgc cggcatcgct gtcgccggcc
     9001 tgctcctcaa gcacggcccc aacagtgaag tagctgattg tcatcagcgc attgacggcg
     9061 tcccggccga aaaacccgcc tcgcagagga agcgaagctg cgcgtcggcc gtttccatct
     9121 gcggtgcgcc cggtcgcgtg ccggcatgga tgcgcgcgcc atcgcggtag gcgagcagcg
     9181 cctgcctgaa gctgcgggca ttcccgatca gaaatgagcg ccagtcgtcg tcggctctcg
     9241 gcaccgaatg cgtatgattc tccgccagca tggcttcggc cagtgcgtcg agcagcgccc
     9301 gcttgttcct gaagtgccag taaagcgccg gctgctgaac ccccaaccgt tccgccagtt
     9361 tgcgtgtcgt cagaccgtct acgccgacct cgttcaacag gtccagggcg gcacggatca
     9421 ctgtattcgg ctgcaacttt gtcatgcttg acactttatc actgataaac ataatatgtc
     9481 caccaactta tcagtgataa agaatccgcg cgttcaatcg gaccagcgga ggctggtccg
     9541 gaggccagac gtgaaaccca acagacccct gatcgtaatt ctgagcactg tcgcgctcga
     9601 cgctgtcggc atcggcctga ttatgccggt gctgccgggc ctcctgcgcg atctggttca
     9661 ctcgaacgac gtcaccgccc actatggcat tctgctggcg ctgtatgcgt tgatgcaatt
     9721 tgcctgcgca cctgtgctgg gcgcgctgtc ggatcgtttc gggcggcggc cggtcttgct
     9781 cgtctcgctg gccggcgctg ctgtcgacta cgccatcatg gcgacggcgc ctttcctttg
     9841 ggttctctat atcgggcgga tcgtggccgg catcaccggg gcgactgggg cggtagccgg
     9901 cgcttatatt gccgatatca ctgatggcga tgagcgcgcg cggcacttcg gcttcatgag
     9961 cgcctgtttc gggttcggga tggtcgcggg acctgtgctc ggtgggctga tgggcggttt
    10021 ctccccccac gctccgttct tcgccgcggc agccttgaac ggcctcaatt tcctgacggg
    10081 ctgtttcctt ttgccggagt cgcacaaagg cgaacgccgg ccgttacgcc gggaggctct
    10141 caacccgctc gcttcgttcc ggtgggcccg gggcatgacc gtcgtcgccg ccctgatggc
    10201 ggtcttcttc atcatgcaac ttgtcggaca ggtgccggcc gcgctttggg tcattttcgg
    10261 cgaggatcgc tttcactggg acgcgaccac gatcggcatt tcgcttgccg catttggcat
    10321 tctgcattca ctcgcccagg caatgatcac cggccctgta gccgcccggc tcggcgaaag
    10381 gcgggcactc atgctcggaa tgattgccga cggcacaggc tacatcctgc ttgccttcgc
    10441 gacacgggga tggatggcgt tcccgatcat ggtcctgctt gcttcgggtg gcatcggaat
    10501 gccggcgctg caagcaatgt tgtccaggca ggtggatgag gaacgtcagg ggcagctgca
    10561 aggctcactg gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc tcttcacggc
    10621 gatctatgcg gcttctataa caacgtggaa cgggtgggca tggattgcag gcgctgccct
    10681 ctacttgctc tgcctgccgg cgctgcgtcg cgggctttgg agcggcgcag ggcaacgagc
    10741 cgatcgctga tagaggttta tagcggcttc gttcccttcc gcaaccgtca gcatgatctg
    10801 ctccacatgc tcgcggccat agtctatcac cgcccgcagc agcataccgg cgacccctcg
    10861 gcctcgctgt tcgggctcca cgaaaacgcc ggacagatgc gccttgtgag cgtccttggg
    10921 gccgtcctcc tgtttgaaga ccgacagccc aatgatctcg ccgtcgatgt aggcgccgaa
    10981 tgccacggca tctcgcaacc gttcagcgaa cgcctccatg ggctttttct cctcgtgctc
    11041 gtaaacggac ccgaacatct ctggagc
//
