LOCUS       AP031284                4447 bp    DNA     circular BCT 08-MAR-2024
DEFINITION  Enterococcus faecium NUITM-VRE32 plasmid pNUITMV32_8 DNA, complete
            sequence.
ACCESSION   AP031284
VERSION     AP031284.1
DBLINK      BioProject: PRJDB10427
            BioSample: SAMD00639561
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE32
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2024) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 99x
            Sequencing Technology :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE32"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMV32_8"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            complement(43..966)
                     /locus_tag="NUITMVRE32_31840"
     CDS             complement(43..966)
                     /locus_tag="NUITMVRE32_31840"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH10106.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEIIF
                     SFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDKQL
                     VIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKEKFF
                     DLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTWKAE
                     INHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQQQQTE
                     EKQAEKRELSNKLKQDLLGNLQNLFD"
                     /name="hypothetical protein"
     gene            complement(2256..2690)
                     /locus_tag="NUITMVRE32_31850"
     CDS             complement(2256..2690)
                     /locus_tag="NUITMVRE32_31850"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH10107.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="hypothetical protein"
     gene            complement(3095..3268)
                     /locus_tag="NUITMVRE32_31860"
     CDS             complement(3095..3268)
                     /locus_tag="NUITMVRE32_31860"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BFH10108.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     Typing          complement(44..966)
                     /name="rep_cluster_1291"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagttctttg attgttttat ctgccataaa tactccttaa gcttaatcga ataagttttg
       61 taaatttcct agtaaatctt gtttgagttt gtttgaaagt tctcgttttt ctgcctgttt
      121 ttcttcagtt tgttgttgtt gtttaaaatc agcttcatga gtgcctaagg gaagatttag
      181 tattcgatct ttagcgcgcc atttttcttc tcgtgttaat tctccattgt gatcaatatt
      241 aaataatttt atacgtaaat cttcttgttt ccctttggaa aaatcgtcag catggttaat
      301 ttcagctttc caagtaaaac gataaccgat aacaggttta cctcgacctt taccatattt
      361 tttacgaata gttaatcctt taaataaggg agtgagttct tctttaattg gccttaagat
      421 aaatttgtca atatttgacg gactattcca ataactttta ggaatatcta gtaaatcaaa
      481 aaatttttct ttggaaaatt cagtccagcc ttgagttcga tattgtttta ataatcgaaa
      541 catagttttt gaatatgtgc tatccaaatt agtaaattgc tctagattaa atcttgtcca
      601 gttctttaat tgattaaaaa gtcctttaaa ttctggatta actgaaatta taacaagttg
      661 tttatctcgt ataatgctat attttgtgaa agcaacaaat ttagttaaag tatttccatc
      721 atcattccaa gcattaatac ttaataattt gtcatacgtg ctactaagat cttttataaa
      781 ttgttcccca cgttctgtat aaccgcttag ttttttcaaa tcgtgaaatg aaaaaataat
      841 ctcatctaca cctttttcac gtacacgtga agcaattgaa aaaaataagt tcatttcacg
      901 actattaaat tttctcagag gaatatgatt taatctattt tcatatttta caatttcatt
      961 tgccataagc ttctcctact tttataaagt atgaaataaa agtaacatac tttataaaaa
     1021 tactcaataa tgtcggaaaa cctacttgaa aagcgtagaa aacctacttg aaaagcgtag
     1081 aaaacctact tgaaaagcgt agaaaaccta cttgaaaaaa ctctgaattg cttgggagag
     1141 taaggacaca gccgacataa aagagatata aaaagagata taaaaagaga tataaaaaga
     1201 ggggaggcta attttcccgc ctaaaaaatc actccttttt tctctaaaca ggttcgcgaa
     1261 tttatcaaaa ctgccaattc caaaccttgc tccctacggt tcgctaaaaa ggtgtgatcg
     1321 ctctgctctc acattatact gcgcagtcgc ctccggctcc tttgctaaaa gccgttcata
     1381 aggaaccctt tgagaagctc tgagcgttcg tttaagacat tttaagacag tcctagtata
     1441 aatcccttat cttgttctta aaacgcctta caggcggtca gggcaagccc taacaaccct
     1501 aaaattgtct aaatgccctc taaaattcga tttaagcact cttaaaccta ttcttatata
     1561 tttatatact taggcgtttc aaacgtctta aaacgcaaat tagagcttga taaagatatt
     1621 tgctcgtgtg cctagacttt taaaaaagtc ggcacacgcc atgacaaagc tgaaataaac
     1681 tgcttcagct ttgtcatggc ggattcgctc acttgaaaaa gtgtttattt cgctcgatta
     1741 ttagcatata ttttttagct tttttgctag ccaaattttg cattaatttg ctcataagtc
     1801 ccaccctttc agtacgtcct acggacgtat cgttcgtaat gtgaaagtac ctttaaaggt
     1861 actttctaca aaactcacta aaagggtttg gcaagctcta tatttgctag ccaaatacct
     1921 ccgtatttgg tgcaaataac cgcttgtcaa aggggcgcaa gctccccctt gaatccccct
     1981 caaaacccac gctccacaat acaaaaagtg gctagaaaat gctagccact ttttgtaaaa
     2041 tttagtatga tttattttta gctatttaga gtttttattt ccgtaaatac ttctaaaaat
     2101 tgctaagata aatgctatag gaattagata aagaatactt ttttgtaagc ttatattgaa
     2161 aattagccta aatatgaaga acatagtgat taaaactatt attgaaataa ttacagctaa
     2221 aatcttttta ttcatgagat agtttcactt tctgattatt ttattacata acgagcgtaa
     2281 tcattgtatt gatatgatgt tgtattgcta aagtgagcat tatatgttaa ttgtacacct
     2341 tttccttgat ttgcagctct tacaaagaca ttcattgcat caacagcact actaaaagca
     2401 tttgtaataa cccatgttcc aactccacca ataggaataa aagatgcaat ttgagcagga
     2461 gtctttagct tattataatt ttttttcaaa tctctacaca ttttattagt aacataatag
     2521 ttcattttat agcaagtaaa tccacgatga ttatgattat atcgtgaact tgcagcttcc
     2581 acttttatac ctctagcact aaaaggaaac agatttaacg gagtacttgc tccaacaatc
     2641 ataccgaaca aaaatagaca agtaataatt ttaataattt tatttttcat tgttacttcc
     2701 tccttagata taaaatccta ggaaagcgct ttctgtatat atttagaata atcagaaaat
     2761 taaatattgt caagagggtt tattttataa aacgtgttat tttaaaggtt agtagacata
     2821 atgtcgagta tgccaagtag cccttgtatc aaccgataca ggggctcact ttgtgatttt
     2881 tcattggtct aggtaaaaag ctctttttaa ggcttgattt tctgcagttg cttctttccg
     2941 tttctttgtg aatttttacc agtcattttt tactggattg ttatttttca taaaatatga
     3001 taaatttttg gtagcgcttt ataagcgcgt tgctttttca agaataaaca ctcattattt
     3061 tttaactggc tgctcggaag agcagtcttt tttattattt atccgtttct tttacaaaca
     3121 attcttcgtt aggactgttg taataatttg aattcaattt aagttctctg ttggaattgt
     3181 tttttccaat tacggataat agcatggggc taaaacctaa tattcctaga gaaatagaac
     3241 ccagtaccaa cacagttttt tttaacatag gttatacctc cacttttttt aacgctacca
     3301 cttgtataca cattttaaat actagccttc agtttatcaa atgtttaaat tgaacggata
     3361 taaatcaaaa ttaaattttc aaaagttgtt ctaaaaaatt tttctttttt ggaagttact
     3421 ctaacttttt ttgcttgtgg tgcttggtat gttttctccg cctcccaggt tcttgaccat
     3481 tttctctcaa cgtggtaaaa atcccactta taagagaaaa tggtcaagaa cctgggaggc
     3541 ggagaatgcc cttaatacca agcaccgcaa gcctttccca tactttattc gatgatccca
     3601 cgatgatctc ataatgattc tacaatgacc ctacgatgat caaaattctc aaaaaaaata
     3661 aacgaaattt caaacgattt ttttgaaatt ttgttaaaat cacatttgtt tttttatgac
     3721 tttttcaaca aaatcgatta gaatttattt tgtttttcca ccaaaaattg agtgagattt
     3781 caacctattt taagcaataa gtcaccatat agtttgatag tttacaacta taaacattga
     3841 ttgtttacag ttgtaaacta ttttttcttt ctcataaaaa tttgagtgat ctccccatca
     3901 ggtagtacaa ctttttcctc tactatccga actaacttct agaaaatttt agagattggt
     3961 tctattagat ttccaaattt gccaccactt tttttcaata agtggttgtg tattgtctgc
     4021 ttttttttca gaaaaggcag ttgattcggt tgttggagtt tcttctgtat aagttaaatg
     4081 gagctgttct tgtaggttct caatttgttg gttcgcttgt agagtgagtt gttgttgctg
     4141 gtcgagtaat ttttgcattt gttttatttg attatctttt tcttgtagct gtatatcttt
     4201 tcgatttaac tgtttttcta ggaaagaaag gagttttaat tcggaaactt ggttggtgtt
     4261 tttatggttt ttggtttttg atttttcgcc aactagtttt cgatttaagt ttgcgttatc
     4321 gactaaaaac attgatttta tagctttttg accggtttcg ttaatcacaa acttgtttcc
     4381 gttttttgca aaccaaagtt ttcgattttt gtcagaaact tttttgttaa ttgctgtttt
     4441 actaact
//
